Detection kits and nucleic acid detection devices

Abstract

The present invention relates to a detection box and a nucleic acid detection device, wherein the detection box includes: a plurality of reagent cells and at least one sample cell; a reaction cell; a connecting valve, wherein the connecting valve includes a plurality of channels, and the connecting valve can be rotated to connect the reaction cell with any one of the reagent cells or the sample cell through the channels at corresponding positions; a driving member, wherein the driving member is connected to the reaction cell, and the driving member is used to draw the reagent in the reagent cell or the sample in the sample cell into the reaction cell, or to discharge the substance in the reaction cell into the reagent cell or the sample cell. The nucleic acid detection device includes the above-mentioned detection box. The structure of the above-mentioned detection box is relatively simple, can reduce manufacturing costs, and is convenient for storage and transportation.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a detection box and a nucleic acid detection device. Background Art

[0002] Many chemical or biological experiments and tests require multi-step sample processing. For example, nucleic acid testing requires multiple steps, including lysis, binding, washing, elution, and amplification. Dedicated test kits are typically designed to perform these experiments and tests. However, many current test kits are complex, resulting in high manufacturing costs and inconvenient storage and transportation. Summary of the Invention

[0003] Based on this, the present invention proposes a detection box, which has a simple structure, can reduce manufacturing costs, and is easy to store and transport.

[0004] A detection kit, comprising:

[0005] A plurality of reagent cells and at least one sample cell;

[0006] reaction tank;

[0007] a communication valve, the communication valve comprising a plurality of channels, the communication valve being rotatable so as to allow the reaction pool to communicate with any one of the reagent pools or the sample pool through the channels at corresponding positions;

[0008] A driving member is connected to the reaction pool, and is used to draw the reagent in the reagent pool or the sample in the sample pool into the reaction pool, or to discharge the substance in the reaction pool into the reagent pool or the sample pool.

[0009] In one embodiment, the communication valve is columnar, and the channel is provided on a peripheral surface of the communication valve.

[0010] In one embodiment, the head end of each channel is arranged along the rotation direction of the communication valve, and the end end of each channel can be connected to the corresponding reagent pool when the communication valve is rotated to a predetermined position.

[0011] In one embodiment, the reaction pool and at least part of the reagent pool are arranged along the length direction of the connecting valve.

[0012] In one embodiment, at least part of the channel includes a first section, a second section and a third section, the first section is used to communicate with the reaction tank, the third section is used to communicate with the reagent tank, the first section and the third section are both connected to the second section, the first section extends along the length direction of the connecting valve, and the first section and the third section are collinear.

[0013] In one embodiment, the detection box further includes a plurality of amplification reaction pools, the reaction pools and the amplification reaction pools are respectively located on opposite sides of the connecting valve along the radial direction of the connecting valve, and the plurality of amplification reaction pools are arranged along the length direction of the connecting valve.

[0014] In one embodiment, at least part of the channel includes a fourth section and a fifth section, the fourth section is used to connect with the reaction pool, the fifth section is used to connect with the amplification reaction pool, the fourth section extends along the length direction of the connecting valve, and the fifth section extends along the length direction and rotation direction of the connecting valve.

[0015] In one embodiment, the detection box also includes a detection box body, the interior of the detection box body is provided with a receiving groove, the connecting valve is located in the receiving groove, the interior of the detection box body is provided with multiple cavities to form the reaction pool and the reagent pool, and the amplification reaction pool is connected to the outside of the detection box body.

[0016] In one embodiment, a magnetic member is further included, and the magnetic member is connected to the detection box body. The reaction pool is used to accommodate the binding liquid and magnetic particles, and the magnetic member is used to adsorb the magnetic particles.

[0017] In one embodiment, the apparatus further comprises a stirrer connected to the sample pool, and the stirrer can move relative to the sample pool to stir.

[0018] The above-mentioned detection box is provided with a reaction pool and a plurality of reagent pools. Reagents can be placed in the reagent pools, and the reaction pools are used for reacting the reagents with the samples. A plurality of channels are provided on the connecting valve. When the connecting valve is rotated, the reaction pool and different reagent pools can be connected through the corresponding channels. The driving member is connected to the reaction pool, which can draw the reagent from the reagent pool into the reaction pool for reaction, or discharge the waste liquid after the reaction is completed from the reagent pool into the reaction pool. It is only necessary to rotate the connecting valve to connect the reaction pool with different reagent pools, and the reagent can flow between the reaction pool and the reagent pool by driving the driving member to complete the reaction. The detection box has a small number of parts and a simple structure, which makes it easy to store and transport, and can reduce the manufacturing cost to a certain extent.

[0019] The present invention also provides a nucleic acid detection device, wherein the accompanying detection box has a simple structure, can reduce manufacturing costs, and is convenient for storage and transportation.

[0020] A nucleic acid detection device comprises the above-mentioned detection box.

[0021] The above-mentioned nucleic acid detection device, wherein the supporting detection box is provided with a reaction pool and a plurality of reagent pools, reagents can be placed in the reagent pool, and the reaction pool is used for the reagents to react with the sample. A plurality of channels are provided on the connecting valve, and when the connecting valve is rotated, the reaction pool and different reagent pools can be connected through the corresponding channels. The driving member is connected to the reaction pool, and it can draw the reagent from the reagent pool into the reaction pool for reaction, or discharge the waste liquid after the reaction is completed from the reagent pool into the reaction pool. It is only necessary to rotate the connecting valve to connect the reaction pool with different reagent pools, and the reagent can flow between the reaction pool and the reagent pool by driving the driving member to complete the reaction. The detection box has a small number of parts and a simple structure, which is convenient for storage and transportation, and can reduce the manufacturing cost to a certain extent, thereby reducing the cost of the entire detection device accordingly. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Schematic diagram of the overall structure of the detection box in one embodiment of the present invention;

[0023] Figure 2 for Figure 1 A schematic structural diagram of the detection box body of the detection box;

[0024] Figure 3 for Figure 1 Schematic diagram of the structure of the amplification reaction pool component of the detection box;

[0025] Figure 4 for Figure 1 A schematic structural diagram of the driving member of the detection box;

[0026] Figure 5 for Figure 1 A schematic diagram of the structure of the connecting valve of the detection box;

[0027] Figure 6 for Figure 1 A schematic structural diagram of the connecting valve of the detection box from another angle;

[0028] Figure 7 for Figure 1 Schematic diagram of the structure of the connecting valve of the detection box from another angle.

[0029] Reference numerals:

[0030] Detection cartridge body 100, sample reservoir 110, first connecting portion 111, top cover 112, second reagent reservoir 120, second connecting portion 121, third reagent reservoir 130, third connecting portion 131, fourth reagent reservoir 140, fourth connecting portion 141, fifth reagent reservoir 150, fifth connecting portion 151, sixth reagent reservoir 160, sixth connecting portion 161, seventh reagent reservoir 170, seventh connecting portion 171, receiving tank 180, eighth connecting portion 181, reaction reservoir 190, ninth connecting portion 191;

[0031] connecting valve 200, first channel 210, second channel 220, third channel 230, first section 231, second section 232, third section 233, first transition section 234, second transition section 235, fourth channel 240, fifth channel 250, sixth channel 260, seventh channel 270, eighth channel 280, ninth channel 290, fourth section 291, fifth section 292, tenth channel 2100, eleventh channel 2110, twelfth channel 2120, thirteenth channel 2130, fourteenth channel 2140, fifteenth channel 2150, and groove 2160;

[0032] Driving member 300, driving member body 310, inner cavity 311, tenth connecting portion 312, push-pull rod 320, sealing portion 330;

[0033] Amplification reaction pool assembly 400, fixing plate 410, first amplification reaction pool 421, second amplification reaction pool 422, third amplification reaction pool 423, fourth amplification reaction pool 424, fifth amplification reaction pool 425, sixth amplification reaction pool 426, seventh amplification reaction pool 427, and eighth amplification reaction pool 428. DETAILED DESCRIPTION

[0034] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0035] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0036] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0037] In the present invention, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0038] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0039] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

[0040] See Figure 1 and Figure 2 , respectively, show a schematic diagram of the overall structure of a test kit according to one embodiment of the present invention and a schematic diagram of the structure of the test kit body. The test kit provided in one embodiment of the present invention includes components such as a test kit body 100, a connecting valve 200, a driver 300, and an amplification reaction cell assembly 400. This test kit can be used for chemical or biological testing, for example, nucleic acid testing. In this embodiment, the test kit is described using nucleic acid testing as an example.

[0041] The test kit body 100 is L-shaped and contains multiple cavities, which form various reagent reservoirs and reaction pools. Specifically, eight cavities are located within the test kit body 100, forming the sample pool 110, the second reagent pool 120, the third reagent pool 130, the fourth reagent pool 140, the fifth reagent pool 150, the sixth reagent pool 160, the seventh reagent pool 170, and the reaction pool 190. The sample pool 110 can hold a sample, and each reagent pool contains a corresponding reagent. The reaction pool 190 provides a reaction space for the sample and reagents.

[0042] Specifically, the sample pool 110 contains a cell lysis solution for lysing cells, viruses, etc. to release nucleic acid substances. The top of the sample pool 110 is provided with a top cover 112. By opening the top cover 112, the sample can be placed therein. The reaction pool 190 contains a binding liquid and magnetic particles, which are used to adsorb nucleic acids. The second reagent pool 120, the third reagent pool 130 and the fourth reagent pool 140 contain a cleaning solution for cleaning the lysed nucleic acids and removing impurities therein. The fifth reagent pool 150 contains an eluent for eluting and separating the nucleic acids adsorbed on the magnetic particles. The sixth reagent pool 160 contains a diluent for diluting the eluent containing nucleic acids to meet the concentration requirements. The seventh reagent pool 170 contains a PCR reaction solution for providing the required enzymes, magnesium ions, DNTP and other substances for the PCR reaction.

[0043] It should be noted that the positional relationship between the reaction pool 190 and each reagent pool and sample pool is not limited. The accompanying drawings only provide one position arrangement method. Other methods can also be used. For example, the position of the reagent pool for the cleaning liquid and the reagent pool for the eluent can be interchanged.

[0044] The detection cartridge body 100 also has a receiving groove 180 with an opening at one end. The communication valve 200 is inserted into the receiving groove 180 through the opening. The communication valve 200 and the detection cartridge body 100 are tightly fitted. During manufacturing and assembly, the gap between the two is controlled to ensure that the communication valve 200 can rotate within the receiving groove 180 without falling out.

[0045] The connecting valve 200 is provided with multiple channels. When the connecting valve 200 is rotated, the reaction pool 190 can be connected with the reagent pool or sample pool 110 at different positions through the corresponding channels, so that the reagents and samples can flow between the reaction pool 190 and the reagent pool or sample pool 110.

[0046] The driver 300 is fixedly connected to the detection cartridge body 100. For example, the two can be fixed by bonding or snap-fit ​​connection. The driver 300 is connected to the reaction cell 190. The driver 300 can be used to draw reagents stored in each reagent cell into the reaction cell 190, or to discharge waste liquid after the reaction in the reaction cell 190 into the corresponding reagent cell.

[0047] Preferably, the driver 300 is fixed to the L-shaped notch of the detection box body 100, and its size matches the notch. When the driver 300 and the detection box body 100 are assembled, the overall shape of the two is a regular rectangular parallelepiped, making the detection box easy to store and transport.

[0048] When performing nucleic acid testing, the test kit only needs to rotate the connecting valve 200 to connect the reaction pool 190 with each reagent pool or sample pool 110 through the corresponding channels on the connecting valve 200. Then, through suction by the driving member 300, the flow of substances between the reaction pool 190 and each reagent pool or sample pool 110 can be achieved. The entire test kit has a small number of components and a simple structure, which can reduce manufacturing costs to a certain extent and reduce the difficulty of operation.

[0049] Preferably, the above components are made of plastic, so that the entire detection box is light and low in cost. The components can be integrally injection molded, or they can be separately molded and then fixed by bonding or other methods.

[0050] Preferably, the accommodating groove 180 is a columnar blind hole, the connecting valve 200 is columnar, and its outer surface is tightly fitted with the groove wall of the accommodating groove 180 , and each channel is arranged on the peripheral surface of the connecting valve 200 .

[0051] Because the connecting valve 200 is cylindrical and has a large circumference, it can accommodate a greater number of channels, allowing for the installation of a larger number of reagent or sample cells 110. Furthermore, to establish communication between the reaction cell 190 and each reagent or sample cell 110, simply rotate the connecting valve 200, without having to move it to pull it out or push it in. This reduces the overall length of the test cartridge along the connecting valve 200 and minimizes the space it occupies. During this process, there is no need to move the reaction cell 190 or each reagent or sample cell 110, making operation very simple.

[0052] See Figure 4 , showing Figure 1 Schematic diagram of the structure of the driving member of the detection box. The driving member 300 includes a driving member body 310, a push-pull rod 320 and a sealing part 330. An inner cavity 311 is provided inside the driving member body 310, and the inner cavity 311 is a cylindrical open groove. One end of the push-pull rod 320 extends into the inner cavity 311, and the other end is located outside the driving member body 310. The sealing part 330 is located in the inner cavity 311, and the sealing part 330 is fixedly connected to the end of the push-pull rod 320. The sealing part 330 can be made of a material such as rubber or silicone, and the peripheral surface of the sealing part 330 is interference fit with the cavity wall of the inner cavity 311 to achieve sealing, thereby separating the inner cavity 311 from the outside.

[0053] The driving member body 310 is further provided with a tenth connecting portion 312, the top end of the tenth connecting portion 312 is communicated with the inner cavity 311, and the bottom end (the top end and the bottom end here refer to Figure 4 An opening is formed on the surface of the driver body 310 (perspective view). When the driver 300 is assembled to the detection cartridge body 100, the bottom end of the tenth connecting portion 312 is located at the reaction pool 190, and the inner cavity 311 is connected to the reaction pool 190 through the tenth connecting portion 312.

[0054] The driving member 300 can be directly modified with a syringe, and a hole is set on the syringe to form the tenth connecting portion 312, which has low manufacturing difficulty.

[0055] See Figure 1 、 Figures 5 to 7, respectively, show schematic structural diagrams of the connecting valve from different angles. Preferably, the connecting valve 200 is provided with grooves on its circumference to form the aforementioned channels. The reaction pool 190 is connected to each reagent pool via the grooves. To manufacture the connecting valve 200, it is only necessary to select a columnar object of appropriate size and remove material from its surface to form the grooves. This makes the manufacturing process very simple and the manufacturing cost is low.

[0056] Alternatively, a through hole is provided on the circumference of the communication valve 200 to form the above-mentioned passage. The through hole can extend along a straight line or a curve, that is, it can be a straight hole or a curved hole, as long as the through hole has an inlet and an outlet on the circumference of the communication valve 200.

[0057] Specifically, the head ends of the multiple channels on the circumferential surface of the connecting valve 200 are used to connect to the reaction pool 190, and the tail ends are used to connect to the various reagent pools. The head end of each channel is arranged along the rotation direction of the connecting valve 200, that is, the head end of each channel is equidistant from the end of the connecting valve 200. In this way, when the connecting valve 200 rotates, the reaction pool 190 can be connected to the head ends of different channels respectively. Moreover, when the connecting valve 200 rotates and the reaction pool 190 connects to the head end of a channel, the tail end position of the channel just rotates to the corresponding reagent pool and connects to the reagent pool.

[0058] In some embodiments, the reaction pool 190 and at least part of the reagent pool are arranged along the length of the communication valve 200. For example, Figure 2 The sample pool 110 , reaction pool 190 , second reagent pool 120 , third reagent pool 130 , fourth reagent pool 140 , fifth reagent pool 150 , sixth reagent pool 160 and seventh reagent pool 170 are arranged along the length direction of the connecting valve 200 .

[0059] The first channel 210 extends along the length of the connecting valve 200. When the connecting valve 200 is rotated to a certain position, the ninth connecting portion 191 at the bottom of the reaction pool 190 and the first connecting portion 111 at the bottom of the sample pool 110 are respectively connected to the first channel 210 at both ends, thereby establishing communication between the reaction pool 190 and the sample pool 110. The second channel 220 extends along the length of the connecting valve 200 and is positioned offset from the first channel 210. When the connecting valve 200 continues to rotate to the next position, the ninth connecting portion 191 at the bottom of the reaction pool 190 and the second connecting portion 121 at the bottom of the second reagent pool 120 are respectively connected to the second channel 220 at both ends, thereby establishing communication between the reaction pool 190 and the second reagent pool 120.

[0060] When the connecting valve 200 continues to rotate to the next position, the ninth connecting portion 191 at the bottom of the reaction pool 190 and the third connecting portion 131 at the bottom of the third reagent pool 130 respectively connect to the front and rear ends of the third channel 230, thereby establishing communication between the reaction pool 190 and the third reagent pool 130. The third channel 230 includes a first section 231, a second section 232, a third section 233, a first transition section 234, and a second transition section 235. The first section 231 is capable of communicating with the ninth connecting portion 191 at the bottom of the reaction pool 190, and the third section 233 is capable of communicating with the third connecting portion 131 at the bottom of the third reagent pool 130. The first section 231 and the third section 233 are collinear and extend along the length of the connecting valve 200, with the second section 232 being parallel to the first section 231. The first section 231 and the second section 232 are connected by the first transition section 234, and the third section 233 and the second section 232 are connected by the second transition section 235. The first transition section 234 and the second transition section 235 are both inclined relative to the first section 231. The first section 231 and the third section 233 are offset by the second section 232, thereby avoiding the second connecting portion 121 at the bottom of the second reagent reservoir 120, thereby ensuring that only one reagent reservoir is connected to the reaction reservoir 190 at a time.

[0061] Similarly, the first end of the fourth channel 240 can be communicated with the ninth connection portion 191 at the bottom of the reaction cell 190, and the terminal end can be communicated with the fourth connection portion 141 at the bottom of the fourth reagent cell 140. The first end of the fifth channel 250 can be communicated with the ninth connection portion 191 at the bottom of the reaction cell 190, and the terminal end can be communicated with the fifth connection portion 151 at the bottom of the fifth reagent cell 150. The first end of the sixth channel 260 can be communicated with the ninth connection portion 191 at the bottom of the reaction cell 190, and the terminal end can be communicated with the sixth connection portion 161 at the bottom of the sixth reagent cell 160. The first end of the seventh channel 270 can be communicated with the ninth connection portion 191 at the bottom of the reaction cell 190, and the terminal end can be communicated with the seventh connection portion 171 at the bottom of the seventh reagent cell 170.

[0062] The shapes of the fourth channel 240, the fifth channel 250, the sixth channel 260, and the seventh channel 270 are similar to those of the third channel 230, so they are not described in detail. The difference is that the farther away from the reaction pool 190, the more reagent pools need to be avoided, and the longer the middle section of the channel that acts as an offset.

[0063] Of course, the shapes of the individual channels are not limited to the above. Taking the third channel 230 as an example, the first section 231 and the third section 233 can be connected by a curved second section 232. For example, the second section 232 can be arc-shaped. The first section 231 and the third section 233 do not necessarily extend along the length of the connecting valve 200. The specific shape can be adjusted based on the location and number of reagent cells, as long as the head and tail ends are connected to the corresponding reaction cell 190 and reagent cell, respectively, and avoid other reagent cells.

[0064] See Figure 1 and Figure 3 , Figure 3 Shown Figure 1 Schematic diagram of the structure of the amplification reaction pool assembly of the detection box. The reaction pool 190 and the amplification reaction pool assembly 400 are respectively located on the opposite side of the connecting valve 200 along the radial direction of the connecting valve 200, and the amplification reaction pools are arranged along the length direction of the connecting valve 200. Specifically, Figure 3 The amplification reaction pool assembly 400 is shown fixed to the bottom of the detection box body 100. The amplification reaction pool assembly 400 includes a fixed plate 410 and multiple amplification reaction pools. The amplification reaction pools are fixed to the fixed plate 410, and the fixed plate 410 is fixedly connected to the bottom of the detection box body 100. The amplification reaction pools store primers for PCR reactions, which can amplify nucleic acids. Because the amplification reaction pools are placed outside the detection box body 100, they are more convenient to operate during subsequent fluorescence detection.

[0065] Similar to the aforementioned reagent pool, the head end of the channel used to connect the reaction pool 190 with each amplification reaction pool also needs to be arranged along the rotation direction of the connecting valve 200, and the end can reach the corresponding amplification reaction pool when the connecting valve 200 is rotated to a predetermined position.

[0066] In some embodiments, the eighth channel 280 extends along the rotation direction of the connecting valve 200. When the connecting valve 200 rotates to connect the head end of the eighth channel 280 with the ninth connecting portion 191 at the bottom of the reaction pool 190, the end of the eighth channel 280 reaches the first amplification reaction pool 421.

[0067] The ninth channel 290 includes a fourth section 291 and a fifth section 292. The fourth section 291 can communicate with the reaction reservoir 190, and the fifth section 292 can communicate with the second amplification reaction reservoir 422. The fourth section 291 extends along the length of the communication valve 200, while the fifth section 292 extends in a curved shape, extending along both the length and the rotational direction of the communication valve 200.

[0068] Similarly, the first end of the tenth channel 2100 can be communicated with the ninth connection portion 191 at the bottom of the reaction pool 190, and the terminal end can be communicated with the third amplification reaction pool 423. The eleventh channel 2110 can be communicated with the ninth connection portion 191 at the bottom of the reaction pool 190, and the terminal end can be communicated with the fourth amplification reaction pool 424. The first end of the twelfth channel 2120 can be communicated with the ninth connection portion 191 at the bottom of the reaction pool 190, and the terminal end can be communicated with the fifth amplification reaction pool 425. The first end of the thirteenth channel 2130 can be communicated with the ninth connection portion 191 at the bottom of the reaction pool 190, and the terminal end can be communicated with the sixth amplification reaction pool 426. The first end of the fourteenth channel 2140 can be communicated with the ninth connection portion 191 at the bottom of the reaction pool 190, and the terminal end can be communicated with the seventh amplification reaction pool 427. The first end of the fifteenth channel 2150 can be communicated with the ninth connection portion 191 at the bottom of the reaction pool 190, and the terminal end can be communicated with the eighth amplification reaction pool 428.

[0069] In addition, a plurality of eighth connecting portions 181 are provided at the bottom of the connecting valve 200 for connecting the channel ends on the connecting valve 200 with the amplification reaction pools at corresponding positions.

[0070] Preferably, the connecting positions between the sections in the channel are all rounded to ensure smoother flow of reagents and waste liquid in the channel.

[0071] Preferably, a pointer is provided at the end of the connecting valve 200, and a scale is provided at a corresponding position on the detection cartridge body 100. When the reaction pool 190 is connected to each reagent pool or amplification reaction pool, the pointer aligns with the corresponding scale line. Therefore, when turning the connecting valve 200, it is only necessary to align the pointer with the corresponding scale line, which is very convenient.

[0072] Preferably, a magnetic member (not shown) is provided on the outside of the reaction pool 190 to adsorb and fix magnetic particles in the binding solution within the reaction pool 190. The magnetic member can be provided on the outside of the detection box body 100, or on the outer wall of the reaction pool 190 within the detection box body. Preferably, the magnetic member is an electromagnet.

[0073] When using the detection box for nucleic acid detection, the sample obtained is first placed in the sample pool 110, mixed with the cell lysate stored therein, and the nucleic acid substance is released. In this process, preferably, an agitator can be set to stir it to accelerate the lysis. The agitator can include a stirring blade and a stirring rod, and the stirring blades are distributed on the stirring rod. The top of the stirring rod can be connected to the top cover 112. After the top cover 112 is covered, it can be connected to the power output end of a driving component such as a motor or a cylinder to drive the agitator to rotate. Alternatively, the agitator can be moved relative to the sample pool 110. When stirring is needed, the agitator is extended into the sample pool. After stirring is completed, the agitator can be removed.

[0074] Alternatively, an ultrasonic device may be provided to accelerate lysis by ultrasound. Alternatively, a heating device may be provided to accelerate lysis by increasing the temperature. Alternatively, a combination of the above methods may be employed.

[0075] After the cell lysis is completed, the connecting valve 200 is turned until the sample pool 110 is connected to the reaction pool 190. Then, the push-pull rod 320 is pulled to suck all the substances in the sample pool 110 into the reaction pool 190. The reaction pool 190 contains magnetic particles and binding liquid. At this time, the nucleic acid will be adsorbed on the surface of the magnetic particles in the reaction pool 190. Then, the electromagnet is energized to adsorb and fix the magnetic particles, so that both the magnetic particles and the nucleic acid are fixed on the inner wall of the sample pool 110. Then, the push-pull rod 320 is pushed to reset it. During this process, the waste liquid in the reaction pool 190 will be pressed into the sample pool 110, and the nucleic acid will be retained in the reaction pool 190.

[0076] Then continue to rotate the connecting valve 200 until the second reagent pool 120 is connected to the reaction pool 190. Pull the push-pull rod 320 to suck the cleaning liquid in the second reagent pool 120 into the reaction pool 190. The cleaning liquid can clean the nucleic acid and remove other impurities. During cleaning, the electromagnet is powered off to release the fixation of the magnetic particles. This increases the contact area between the nucleic acid and the cleaning liquid and optimizes the cleaning effect. Then, the electromagnet is energized to adsorb and fix the magnetic particles, push the push-pull rod 320 to reset it, and the cleaning liquid returns to the second reagent pool 120 again. Repeat the above steps, and the cleaning liquid oscillates and impacts multiple times between the second reagent pool 120 and the reaction pool 190 to optimize the cleaning effect.

[0077] Similarly, the third reagent pool 130 and the fourth reagent pool 140 are connected to the reaction pool 190 respectively in the above manner, and the nucleic acid is continuously cleaned by the cleaning solution therein. Through multiple cleanings, impurities can be removed more thoroughly.

[0078] After cleaning is completed, turn the connecting valve 200 until the fifth reagent cell 150 is connected to the reaction cell 190. Pull the push-pull rod 320 to suck the eluent in the fifth reagent cell 150 into the reaction cell 190. The eluent can separate the nucleic acid from the magnetic particles, making them free in the eluent. At the same time, the electromagnet is energized to adsorb and fix the magnetic particles. Pull the push-pull rod 320 back and forth to oscillate the cleaning liquid between the fifth reagent cell 150 and the reaction cell 190 to fully elute the nucleic acid. Finally, keep the push-pull rod 320 pulled out, and the eluent and nucleic acid remain in the reaction cell 190.

[0079] Since the nucleic acid concentration in the eluate is high at this point, it needs to be diluted to meet the requirements of subsequent steps. Turn the connecting valve 200 until the sixth reagent reservoir 160 is connected to the reaction reservoir 190. Pull the push-pull rod 320 to draw the diluent from the sixth reagent reservoir 160 into the reaction reservoir 190 for dilution.

[0080] After dilution is complete, the connecting valve 200 is turned until the seventh reagent reservoir 170 is connected to the reaction reservoir 190. The push-pull rod 320 is pulled to draw the PCR reaction solution in the seventh reagent reservoir 170 into the reaction reservoir 190, where it is fully mixed with the diluted nucleic acid to prepare for the subsequent PCR reaction.

[0081] Preferably, the size of each channel is smaller than that of the magnetic particles to prevent the magnetic particles from being pushed back into the reagent pools along with the nucleic acids if the magnetic particles are not firmly attached during the waste liquid discharge process. Furthermore, each time the reaction pool 190 is discharged, the electromagnet must securely attach the magnetic particles to prevent them from entering the reagent pools.

[0082] After completing the above steps, the PCR reaction can be carried out. Continue rotating the connecting valve 200 to connect the first amplification reaction pool 421 with the reaction pool 190. Push the push-pull rod 320 to press the substance in the reaction pool 190 into the first amplification reaction pool 421, causing it to react with the primers in the first amplification reaction pool 421 to achieve amplification. Then continue rotating the connecting valve 200 to connect the second amplification reaction pool 422 with the reaction pool 190. Push the push-pull rod 320 to press the substance in the reaction pool 190 into the second amplification reaction pool 422. In this manner, the substance in the reaction pool 190 can be pressed into each amplification reaction pool.

[0083] It should be noted that the same primers or different primers can be placed in each amplification reaction pool, and multiple primers can be placed in a single amplification reaction pool. The primers can be in freeze-dried or liquid form.

[0084] When the material in reaction pool 190 is pushed into each amplification reaction pool, the push-pull rod 320 is only pushed, not pulled, and the material is only discharged. Some material may remain in the channel, causing loss. Therefore, when initially calculating the amount to be used, it is necessary to leave a margin to ensure that there is sufficient amount for the reaction.

[0085] In some embodiments, a nucleic acid detection device is provided, comprising the aforementioned detection kit. After the nucleic acid undergoes PCR reaction, the detection kit is mounted on a nucleic acid detection instrument, which then performs fluorescence detection. Because the aforementioned amplification reaction pool is externally located, fluorescence detection is more convenient.

[0086] Alternatively, a drive device may be provided, connecting the connecting valve 200 to the power output of the drive device, thereby driving the connecting valve 200 to rotate, thereby improving efficiency. A groove 2160 is provided at the end of the connecting valve 200 for connection to the drive device. Alternatively, manual actuation is possible, for example, by providing a handwheel connected to the groove 2160.

[0087] In addition, it should be noted that although the detection kit is introduced by taking nucleic acid detection as an example in the above embodiments, in fact, the detection kit is not limited to nucleic acid detection, and can also be used for other reactions or detections.

[0088] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0089] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A detection kit, characterized in that: include: A plurality of reagent cells and at least one sample cell; reaction tank; a communication valve, the communication valve comprising a plurality of channels, the communication valve being rotatable so as to allow the reaction pool to communicate with any one of the reagent pools or the sample pool through the channels at corresponding positions; A driving member, which is directly connected only to the reaction pool, and is used to draw the reagent in the reagent pool or the sample in the sample pool into the reaction pool through the channel at the corresponding position of the connecting valve, or to discharge the substance in the reaction pool to the reagent pool or the sample pool through the channel at the corresponding position of the connecting valve.

2. The detection box according to claim 1, characterized in that The communication valve is columnar, and the channel is arranged on the peripheral surface of the communication valve.

3. The detection box according to claim 2, characterized in that The head end of each channel is arranged along the rotation direction of the communication valve, and the end end of each channel can be connected with the corresponding reagent pool when the communication valve is rotated to a predetermined position.

4. The detection box according to claim 3, characterized in that The reaction pool and at least part of the reagent pool are arranged along the length direction of the communication valve.

5. The detection box according to claim 4, characterized in that At least part of the channel includes a first section, a second section and a third section, the first section is used to communicate with the reaction pool, the third section is used to communicate with the reagent pool, the first section and the third section are both connected to the second section, the first section extends along the length direction of the connecting valve, and the first section and the third section are collinear.

6. The detection box according to claim 3, characterized in that: The detection box further includes a plurality of amplification reaction pools, which are respectively located on opposite sides of the connecting valve along the radial direction of the connecting valve, and the plurality of amplification reaction pools are arranged along the length direction of the connecting valve.

7. The detection box according to claim 6, characterized in that: At least part of the channel includes a fourth section and a fifth section, the fourth section is used to connect with the reaction pool, the fifth section is used to connect with the amplification reaction pool, the fourth section extends along the length direction of the connecting valve, and the fifth section extends along the length direction and rotation direction of the connecting valve.

8. The detection box according to claim 6, characterized in that: The detection box also includes a detection box body, a receiving groove is provided inside the detection box body, the connecting valve is located in the receiving groove, a plurality of cavities are provided inside the detection box body to form the reaction pool and the reagent pool, and the amplification reaction pool is connected to the outside of the detection box body.

9. The detection box according to claim 8, characterized in that: It also includes a magnetic component, which is connected to the detection box body. The reaction pool is used to contain the binding liquid and magnetic particles, and the magnetic component is used to adsorb the magnetic particles.

10. The detection kit according to claim 1, characterized in that: The invention also includes a stirrer connected to the sample pool, and the stirrer can move relative to the sample pool to stir.

11. A nucleic acid detection device, characterized in that: A detection kit comprising the detection kit according to any one of claims 1 to 10.

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