A cell electrostimulation system and device

Abstract

The present application discloses a cell electrostimulation system, which includes an electrostimulation device and a pulse generation device. The pulse generation device is connected to the electrostimulation device and is used to drive the electrostimulation device to generate electrostimulation on cells. The electrostimulation device includes a pulse input terminal, a substrate, and a graphene array electrode; the graphene array electrode is disposed on the substrate and is perpendicular to the substrate; the pulse input terminal is connected to the pulse generation device; the graphene array electrodes are arranged in parallel, the distance between adjacent two graphene array electrodes is equal, and the voltage polarities between adjacent two graphene array electrodes are arranged alternately as positive and negative. The present application adopts vertical graphene array electrodes, effectively increasing the specific surface area of the electrodes and the interfacial electron transport speed, and improving the effectiveness of cell electrostimulation; by simultaneously applying electrostimulation under different conditions to compare the effects of different electrostimulations, the experimental efficiency is improved.

Description

Technical Field

[0001] This application relates to the field of medical devices, and in particular, to a cell electrical stimulation system and device. Background Art

[0002] Cells are the basic structural and functional units of organisms. Therefore, in medical research, cells are often used as experimental objects to explore the pathogenesis of diseases and the therapeutic effects of drugs. Since electrical phenomena accompany the life activities of cells, exploring the effects of electrical stimulation on cell activities has attracted great interest. With the experimental research on cell electrical stimulation at home and abroad, it has been confirmed that electrical stimulation can regulate various functions of cells, but the research on the effects of electrical stimulation on cell activities still requires more in-depth and systematic work.

[0003] In related technologies, most cell electrical stimulation systems use metal materials as electrodes. However, metals will produce electrolysis in cell solutions, interfering with the composition of the solutions. At the same time, the shape of the electrodes will affect the contact area between the electrodes and the solution, and too small a contact area will also have an adverse effect on cell electrical stimulation.

[0004] Therefore, the above technical problems existing in related technologies need to be solved urgently. Summary of the Invention

[0005] This application aims to solve one of the technical problems in related technologies. To this end, embodiments of this application provide a cell electrical stimulation system and device that can improve the effect of electrical stimulation on cells.

[0006] According to one aspect of the embodiments of this application, a cell electrical stimulation system is provided. The system includes an electrical stimulation device and a pulse generation device. The pulse generation device is connected to the electrical stimulation device and is used to drive the electrical stimulation device to generate electrical stimulation for cells. The electrical stimulation device includes a pulse input terminal, a substrate, and a graphene array electrode.

[0007] The graphene array electrode is disposed on the substrate and is perpendicular to the substrate.

[0008] The pulse input terminal is connected to the pulse generation device.

[0009] The graphene array electrodes are arranged in parallel, the distance between adjacent two graphene array electrodes is equal, and the voltage polarities between adjacent two graphene array electrodes are arranged alternately as positive and negative.

[0010] In one embodiment, the pulse generation device has a plurality of channels, and different channels of the pulse generation device correspond to different pulse signal parameters. Among them, the pulse signal parameters at least include the amplitude, frequency, pulse width range of the pulse signal, and the duration of electrical stimulation.

[0011] In one embodiment, the system further includes a host computer, which is connected to the pulse generating device and is used to control different channels of the pulse generating device according to pulse signal parameters to generate corresponding pulse signals and display the current state of the pulse generating device.

[0012] In one embodiment, the amplitude of the pulse signal generated by the pulse generating device is greater than 0 and less than 16V, the frequency range is greater than 0.1Hz and less than 500kHz, and the pulse width range is greater than 1us and less than 4s.

[0013] In one embodiment, the pulse generating device includes a power supply circuit, a USB-to-serial circuit, a plurality of pulse generating circuits, and a terminal block;

[0014] One end of the USB-to-serial circuit is connected to the pulse generating circuit, and the other end is connected to the host computer, and is used to establish a communication connection between the host computer and the pulse generating circuit;

[0015] The pulse generating circuit generates a pulse signal with a corresponding amplitude according to the high and low levels output by the controller. The output end of the pulse generating circuit is connected to the voltage amplification circuit; the voltage amplification circuit is a non-inverting amplifier and is used to amplify the pulse signal.

[0016] In one embodiment, the pulse generating circuit includes a controller circuit, a DAC circuit, a single-pole double-throw switch circuit, and a voltage amplification circuit, and the controller circuit, the DAC circuit, the single-pole double-throw switch circuit, and the voltage amplification circuit are connected in sequence.

[0017] In one embodiment, the controller circuit is used to parse the serial port instructions of the USB-to-serial circuit and control the DAC circuit and the single-pole double-throw switch circuit;

[0018] The DAC circuit includes a reference chip and a DAC chip. The input end of the DAC chip is connected to the controller and is used to receive a 0-3V voltage instruction issued by the controller and generate a corresponding voltage value. The output end of the DAC chip is connected to the input end of the single-pole double-throw switch circuit, and the control end of the single-pole double-throw switch circuit is connected to the IO port of the controller.

[0019] In one embodiment, the system further includes a PCB base, and a plurality of through holes are provided on the PCB base.

[0020] In one embodiment, the substrate is fixed on the PCB base, and the graphene array electrode is connected to the PCB base through conductive silver paste.

[0021] According to one aspect of the embodiments of the present application, a cell electrostimulation device is provided, and the cell electrostimulation device includes a cell electrostimulation system as described in claim 1.

[0022] The beneficial effects of a cell electrostimulation system and device provided by the embodiments of the present application are as follows: The system of the present application includes an electrostimulation device and a pulse generation device. The pulse generation device is connected to the electrostimulation device and is used to drive the electrostimulation device to generate electrostimulation on cells. The electrostimulation device includes a pulse input terminal, a substrate, and a graphene array electrode; the graphene array electrode is disposed on the substrate and is perpendicular to the substrate; the pulse input terminal is connected to the pulse generation device; the graphene array electrodes are arranged in parallel, the distance between adjacent two graphene array electrodes is equal, and the voltage polarities between adjacent two graphene array electrodes are arranged alternately as positive and negative. The present application adopts a vertical graphene array electrode, effectively increasing the specific surface area of the electrode and the interfacial electron transport speed, and improving the effectiveness of cell electrostimulation; by simultaneously applying electrostimulation under different conditions to compare the effects of different electrostimulations, the experimental efficiency is improved. In addition, the electrostimulation device of the present application can be placed in a cell incubator and connected to a pulse generation device placed outside the cell incubator, which can avoid contamination of the cell incubator and maintain the temperature and humidity inside the incubator, ensuring that the activity of cells during the experiment is not affected.

[0023] Additional aspects and advantages of the present application will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and those of ordinary skill in the art can also obtain other drawings based on these drawings without creative efforts.

[0025] Figure 1 Schematic structural diagram of the electrostimulation device provided by the embodiments of the present application;

[0026] Figure 2 Schematic diagram of the graphene array electrode provided by the embodiments of the present application;

[0027] Figure 3 Circuit schematic diagram of the pulse generation device provided by the embodiments of the present application;

[0028] Figure 4 Schematic diagram of the circuit connection of the pulse generation device provided by the embodiments of the present application;

[0029] Figure 5The flowchart of the host computer operation provided by the embodiment of the present application;

[0030] Figure 6 The schematic diagram of the user operation interface of the cell electrostimulation system provided by the embodiment of the present application. Detailed implementation manners

[0031] In order to enable those skilled in the art to better understand the solution of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0032] The terms "first", "second", "third", "fourth", etc. in the specification, claims and drawings of the present application are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally further includes steps or units not listed, or optionally further includes other steps or units inherent to these processes, methods, products or devices.

[0033] Referring to "embodiment" herein means that a specific feature, structure or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0034] As the basic structural and functional unit of an organism, cells are more sensitive to external environmental stimuli. Therefore, experiments with cells as experimental objects have become a common method for exploring the pathogenesis of diseases and the therapeutic effects of drugs. Since cells are accompanied by electrical phenomena during their life activities, exploring the effects of electrical stimulation on cell activities has attracted great interest. With the experimental research on cell electrostimulation at home and abroad, it has been confirmed that electrical stimulation can regulate various functions of cells, but the research on the effects of electrical stimulation on cell activities still requires more in-depth and systematic work.

[0035] Currently, most electrostimulation systems use metal materials as electrodes. However, some metals will electrolyze in cell solutions, interfering with the composition of the solutions. To solve the problem of electrode stability, some systems use graphite materials to replace metal materials. Regarding the shape of the electrodes, in the early stage, most of them adopted the form of electrode wires, with a small contact area with the solution and an unclear electrostimulation effect. To increase the contact area between the electrodes and the solution, subsequent designs adopted sheet electrodes. However, the spacing between the sheet electrodes cannot be made very small, affecting the effectiveness of electrostimulation. Regarding the signal source of the electrostimulation system, most of them use signal generators. The number of channels of the signal generators is limited, which is not conducive to comparing the effects of electrostimulation under different conditions.

[0036] To solve the above problems, this application proposes a cell electrostimulation system and device.

[0037] This application proposes a cell electrostimulation system, including an electrostimulation device and a pulse generation device. The pulse generation device is connected to the electrostimulation device and is used to drive the electrostimulation device to generate electrostimulation on cells. The electrostimulation device includes a pulse input terminal, a substrate, and a graphene array electrode. The graphene array electrode is disposed on the substrate and is perpendicular to the substrate. The pulse input terminal is connected to the pulse generation device. The graphene array electrodes are arranged in parallel, the distance between adjacent two graphene array electrodes is equal, and the voltage polarities between adjacent two graphene array electrodes are arranged alternately as positive and negative.

[0038] Specifically, Figure 1 is a schematic structural diagram of the electrostimulation device provided by an embodiment of this application. As Figure 1 shown, the electrostimulation device includes a pulse input terminal 7, a PCB base 6, a vertical graphene array electrode 2 formed on a glass substrate, and a glass ring 4 located on the glass substrate. The vertical graphene array electrodes are arranged in parallel, the distance between any adjacent two electrodes is equal, and the voltage polarities are arranged alternately as positive and negative. Electrode connection ends are provided on both sides of the glass substrate. Turn the vertical graphene array electrode upwards, fix the glass substrate to the PCB base, and connect the vertical graphene array electrode to the PCB base through the conductive silver paste on the connection end 3. Then, use uncured PDMS to adhere the glass ring 4 above the vertical graphene array electrode as a cell culture chamber. The center of the glass ring should be aligned with the center of the through hole on the PCB base, and it is placed in a hot oven for 2 hours to cure the PDMS.

[0039] In the cell electrostimulation system provided in this embodiment, the material of the array electrode is graphene material, and the installation method of the array electrode is to be disposed on the substrate and perpendicular to the substrate. Among them, the graphene material has excellent electrical conductivity, ultra-high strength, a very large specific surface area, etc. Therefore, using the graphene material in this embodiment can obtain the effect of improving cell stimulation; being vertically installed on the substrate can increase the contact area between the electrode and the solution, while improving the electrical conductivity of the electrode and enhancing the effectiveness of cell electrostimulation.

[0040] It should be noted that the cell electrostimulation system of this embodiment further includes a PCB base, and a plurality of through holes are provided on the PCB base. The substrate is fixed on the PCB base, and the graphene array electrode is connected to the PCB base through conductive silver paste. The function of providing a plurality of through holes on the PCB base is to facilitate observing the state of cells under a microscope, and the function of connecting the graphene array electrode to the PCB base through conductive silver paste is to make each graphene array electrode conduct with each other, and the conductivity of the silver material is relatively high, which can reduce the loss of electrical energy during the conduction process.

[0041] Figure 2 Schematic diagram of the graphene array electrode provided for the embodiment of the present application, as Figure 2 shown, the vertical graphene array electrode 2 is vertically disposed on the glass substrate 1 ( Figure 2 is a top view), and an electrode connection end 3 is provided on the glass substrate 1. It can be seen from Figure 2 that the distance between adjacent two graphene array electrodes is equal, and the voltage polarities of adjacent two graphene array electrodes are arranged alternately as positive and negative. It should be noted that the substrate of this embodiment uses a glass material, and its function is that when observing the cell electrostimulation effect through a microscopic device, the glass material has a high light transmittance, so the microscopic observation clarity is higher.

[0042] Optionally, the pulse generation device in the cell electrostimulation system provided in this embodiment has a plurality of channels, and different channels of the pulse generation device correspond to different pulse signal parameters. Among them, the pulse signal parameters at least include the amplitude, frequency, pulse width range of the pulse signal, and the duration of electrostimulation.

[0043] Optionally, the cell electrostimulation system provided in this embodiment further includes a host computer, which is connected to the pulse generation device and is used to control different channels of the pulse generation device according to pulse signal parameters to generate corresponding pulse signals and display the current state of the pulse generation device. The host computer mainly includes devices such as computers and intelligent terminals that provide human-computer interaction. The connection method between the host computer and the pulse generation device includes wired connection and wireless connection. The host computer sends a control signal to the pulse generation device of this embodiment through a wired connection or a wireless connection. The pulse generation device generates different pulse signals according to the control signal and sends the parameters of the pulse signals to the host computer to display the current state of the pulse generation device. The role of the host computer is that technicians can control the cell electrostimulation system of this application through the host computer. Moreover, the host computer can set the corresponding APP and user interface, further reducing the difficulty of operation for technicians.

[0044] For cell electrostimulation, too high a voltage will cause damage to the cells, and too low a voltage will result in an insignificant cell electrostimulation effect. Therefore, it is a reasonable range that the amplitude of the pulse signal generated by the pulse generation device in this embodiment is greater than 0 and less than 16V, the frequency range is greater than 0.1Hz and less than 500kHz, and the pulse width range is greater than 1us and less than 4s. This can reduce the risk of cells being damaged due to excessive current or voltage while effectively stimulating the cells by driving the electrostimulation device.

[0045] Figure 3 This is the circuit schematic diagram of the pulse generation device provided in the embodiment of the present application. Figure 4 This is the circuit connection schematic diagram of the pulse generation device provided in the embodiment of the present application, as Figure 3 and Figure 4 shown, the pulse generation device of this embodiment includes a power supply circuit, a USB to serial port circuit, several pulse generation circuits, and a terminal block; one end of the USB to serial port circuit is connected to the pulse generation circuit, and the other end is connected to the host computer, which is used to establish a communication connection between the host computer and the pulse generation circuit; the pulse generation circuit generates a pulse signal with a corresponding amplitude according to the high and low levels output by the controller, and the output end of the pulse generation circuit is connected to a voltage amplification circuit; the voltage amplification circuit is a non-inverting amplifier, which is used to amplify the pulse signal.

[0046] In the pulse generation device of this embodiment, the power supply circuit provides 18V, 3.3V, and -1.8V required by the device. The 5V power supply voltage is provided by an external power adapter, boosted to 18V through the TPS61175 chip and its peripheral circuit, 3.3V is generated by the linear voltage regulator chip LM1117-3.3, and -1.8V is obtained by boosting through the TPS63710 chip and its peripheral circuit; the USB to serial port circuit uses the CH340 chip to establish a communication connection between the host computer and the pulse generation circuit; the pulse generation circuit consists of a controller circuit, a DAC circuit, a single-pole double-throw switch circuit, and a voltage amplification circuit connected in sequence. The controller circuit uses the chip STM32F103C8T6 as the main control chip to parse the serial port instructions of the USB to serial port circuit and control the DAC circuit and the single-pole double-throw switch circuit. The DAC circuit consists of a reference chip and a DAC chip. The reference chip provides a 3V reference voltage for the DAC chip. The input end of the DAC chip is connected to the controller and is used to receive the 0-3V voltage instruction issued by the controller and generate the corresponding voltage value, and its output end is connected to the input end of the single-pole double-throw switch circuit. The single-pole double-throw switch circuit uses the chip TS5A3157, its other input end is connected to the ground, and the control end is connected to the IO port of the controller. The controller outputs high and low levels to control the single-pole double-throw switch chip, and a pulse signal with the corresponding amplitude can be generated. Its output is connected to the voltage amplification circuit; the voltage amplification circuit is a non-inverting amplifier, which amplifies the pulse signal by 6 times and then outputs it to be connected to the pulse output terminal, which is used as the output end of the pulse generation device and is connected to the electrical stimulation device.

[0047] Specifically, Figure 3 and Figure 4The USB-to-serial circuit chip IC1 in it uses CH340 to convert USB differential signals into serial signals. The VCC pin is connected to the 5V power supply, the V3 pin is connected to the 100nF power decoupling capacitor C4, and the TXD pin and RXD pin are respectively connected to pins 12 and 13 of the controller. The controller 14 is mainly based on the minimum system of the chip STM32F103C8T6, and is used to parse the serial instructions of the USB-to-serial circuit and control the DAC circuit and the single-pole double-throw switch circuit. The DAC circuit consists of chips U3 and U5, and decoupling capacitors C7 and C13. U3 uses the digital-to-analog conversion chip DAC7311 to receive instructions from the controller and convert digital signals into analog signals. U5 uses the reference chip REF3030 to provide a 3V reference voltage for pin 4 of U3, and pin 4 is connected to the decoupling capacitor C7 to ground. Pin 3 of U5 is grounded, and pin 1 is connected to the 3.3V power supply together with the filter capacitor C13. The pulse generation circuit consists of the single-pole double-throw switch chip U2, using the TS5A3157 chip. Pins 2 and 3 of U2 are grounded, pin 5 is connected to the 3.3V power supply together with the decoupling capacitor, pin 1 is connected to pin 6 of U3, and pin 6 is connected to the IO port of the controller 14. The controller 14 outputs high and low levels to control the single-pole double-throw switch chip U2, and corresponding amplitude pulse signals can be generated. The voltage amplification circuit consists of three resistors R4, R5, R6 and the precision operational amplifier U4. The resistor R5 is connected between the inverting input terminal and the output terminal of the precision operational amplifier U4, the resistor R6 is connected between the inverting input terminal of the precision operational amplifier U6 and ground, and the non-inverting input terminal of U4 is connected to pin 4 of U2. Pin 2 of U4 is connected to the power supply -1.8V, and pin 5 is connected to the 18V power supply. The output terminal of U4 is connected to the resistor R11 to the pulse output interface, and the resistor R11 plays a protective role.

[0048] Further, in this embodiment, the pulse generation circuit in the pulse generation device includes a controller circuit, a DAC circuit, a single-pole double-throw switch circuit and a voltage amplification circuit, and the controller circuit, the DAC circuit, the single-pole double-throw switch circuit and the voltage amplification circuit are connected in sequence. The controller circuit is used to parse the serial instructions of the USB-to-serial circuit and control the DAC circuit and the single-pole double-throw switch circuit; the DAC circuit includes a reference chip and a DAC chip. The input terminal of the DAC chip is connected to the controller, and is used to receive the 0-3V voltage instruction issued by the controller and generate the corresponding voltage value. The output terminal of the DAC chip is connected to the input terminal of the single-pole double-throw switch circuit, and the control terminal of the single-pole double-throw switch circuit is connected to the IO port of the controller.

[0049] Figure 5 This is the working flow chart of the host computer provided by the embodiment of the present application. Figure 5 The usage process of the cell multi-path parallel stimulation system based on the vertical graphene array electrode is given. As Figure 5As shown in the figure, first, set the channels to be electrically stimulated and check the "Enable" option in the interface. Then, set the pulse parameters for each channel, including amplitude, frequency, pulse width, and duration. When conducting an electrical stimulation experiment, after clicking the "Start Stimulation" button on the host computer, the host computer packages the configuration information of each module and sends it to the pulse generator through a USB cable. The USB-to-serial circuit on the pulse generator converts the USB differential signal into a serial signal and sends it to the controller of each pulse generation circuit. The controller will parse the serial command to control the generation of corresponding pulses for electrical stimulation on this path.

[0050] In practical applications, first, culture neonatal rat cardiomyocytes in a cell culture chamber. After connecting the electrical stimulation device and the pulse generation device through a flexible cable, place the electrical stimulation device steadily into the cell incubator. Plug in the power supply of the pulse generation device and connect the pulse generation device and the host computer using a USB cable. Open the host computer software, enter the main interface, check the channels to be electrically stimulated, set the pulse parameters for each channel, and click "Start Stimulation". The pulse generation device outputs corresponding pulse signals to electrically stimulate the cardiomyocytes. Initially, the beating frequency of the cardiomyocytes is ~2 Hz. After being stimulated by alternating current with 4 V, 3 Hz, or 4 Hz or 5 Hz, it is found that the beating frequencies of the cardiomyocytes are respectively adjusted to be close to 3 Hz, or 4 Hz or 5 Hz. After being stimulated by alternating current with 6 V, 3 Hz, or 4 Hz or 5 Hz, it is found that the health condition of the cardiomyocytes is relatively poor, mainly because the applied voltage is too high.

[0051] Figure 6 This is a schematic diagram of the user operation interface of the cell electrical stimulation system provided by the embodiment of the present application. Technicians can control the pulse generation circuit by setting the pulse parameters of each channel. At the same time, the host computer can store the pulse parameter information of each channel in the current experiment and can import the parameter information set last time during the next experiment, which is convenient for technicians to improve the convenience of setting experimental parameter information when conducting multiple controlled variable experiments.

[0052] In addition, the present application also provides a cell electrical stimulation device, and the cell electrical stimulation device includes a cell electrical stimulation system described in the previous embodiment.

[0053] In some alternative embodiments, the functions / operations recited in the block diagrams may not occur in the order presented in the operational illustrations. For example, depending on the functions / operations involved, two blocks shown in succession may actually be executed substantially simultaneously or the blocks can sometimes be executed in reverse order. Additionally, the embodiments presented and described in the flowcharts of the present application are provided by way of example for the purpose of providing a more comprehensive understanding of the technology. The disclosed methods are not limited to the operations and logical flows presented herein. Alternative embodiments are contemplated in which the order of various operations is altered and in which sub-operations described as part of a larger operation are performed independently.

[0054] Moreover, although the present application has been described in the context of functional modules, it should be understood that one or more of the functions and / or features may be integrated in a single physical device and / or software module unless otherwise stated to the contrary, or one or more functions and / or features may be implemented in separate physical devices or software modules. It should also be understood that a detailed discussion of the actual implementation of each module is not necessary for an understanding of the present application. Rather, given the attributes, functions, and internal relationships of the various functional modules in the devices disclosed herein, the actual implementation of the modules will be understood within the ordinary skill of an engineer. Thus, those skilled in the art can implement the present application as set forth in the claims without undue experimentation. It should also be understood that the specific concepts disclosed are merely illustrative and are not intended to limit the scope of the present application, which is determined by the full scope of the appended claims and their equivalents.

[0055] If a function is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present application, in essence, or the part that contributes to the prior art, or a part of this technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods of the various embodiments of the present application. The aforementioned storage medium includes: various media such as USB flash drives, mobile hard disks, read-only memories (ROMs), random access memories (RAMs), magnetic disks, or optical discs that can store program codes.

[0056] The logic and / or steps represented in the flowchart or otherwise described herein, for example, can be considered as a definitional sequence list of executable instructions for implementing logical functions, and can be specifically implemented in any computer-readable medium for use by an instruction execution system, apparatus, or device (such as a computer-based system, a system including a processor, or other systems that can fetch and execute instructions from the instruction execution system, apparatus, or device), or in conjunction with these instruction execution systems, apparatus, or devices. For the purposes of this specification, a "computer-readable medium" can be any device that can contain, store, communicate, propagate, or transport a program for use by or in conjunction with an instruction execution system, apparatus, or device.

[0057] More specific examples (a non-exhaustive list) of computer-readable media include the following: an electrical connection portion (electronic device) having one or more wirings, a portable computer diskette (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable medium can even be paper or other suitable media on which a program can be printed, because the program can be obtained electronically, for example, by optically scanning the paper or other media, followed by editing, interpretation, or otherwise processing as appropriate, and then stored in a computer memory.

[0058] It should be understood that various parts of the present application can be implemented by hardware, software, firmware, or a combination thereof. In the above-described embodiments, multiple steps or methods can be implemented by software or firmware stored in a memory and executed by a suitable instruction execution system. For example, if implemented by hardware, as in another embodiment, any one or a combination of the following techniques well known in the art can be used: discrete logic circuits having logic gate circuits for implementing logical functions on data signals, application specific integrated circuits having suitable combinational logic gate circuits, programmable gate arrays (PGAs), field programmable gate arrays (FPGAs), and the like.

[0059] In the above description of this specification, the descriptions referring to the terms "one embodiment / example", "another embodiment / example", or "certain embodiments / examples", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0060] Although embodiments of the present application have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present application. The scope of the present application is defined by the claims and their equivalents.

[0061] In the above, the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit them. Although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments or perform equivalent substitutions for some of the technical features. These modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A cell electrostimulation system, characterized in that, The system includes an electrical stimulation device and a pulse generation device. The pulse generation device is connected to the electrical stimulation device and is used to drive the electrical stimulation device to generate electrical stimulation for cells. The electrical stimulation device includes a pulse input terminal, a substrate, and a graphene array electrode; The graphene array electrode is disposed on the substrate and is perpendicular to the substrate; The pulse input terminal is connected to the pulse generation device; The graphene array electrodes are arranged in parallel, and the distance between adjacent two graphene array electrodes is equal. The voltage polarities between adjacent two graphene array electrodes are arranged alternately as positive and negative; The pulse generation device includes a power supply circuit, a USB-to-serial port circuit, a plurality of pulse generation circuits, and a wiring terminal; One end of the USB-to-serial port circuit is connected to the pulse generation circuit, and the other end is connected to the host computer, and is used to establish a communication connection between the host computer and the pulse generation circuit; The pulse generation circuit generates a pulse signal with a corresponding amplitude according to the high and low levels output by the controller. The output end of the pulse generation circuit is connected to a voltage amplification circuit; the voltage amplification circuit is a non-inverting amplifier and is used to amplify the pulse signal; The pulse generation circuit includes a controller circuit, a DAC circuit, a single-pole double-throw switch circuit, and a voltage amplification circuit. The controller circuit, the DAC circuit, the single-pole double-throw switch circuit, and the voltage amplification circuit are connected in sequence; The controller circuit is used to parse the serial port instructions of the USB-to-serial port circuit and control the DAC circuit and the single-pole double-throw switch circuit; The DAC circuit includes a reference chip and a DAC chip. The input end of the DAC chip is connected to the controller and is used to receive a voltage instruction of 0 - 3V issued by the controller and generate a corresponding voltage value. The output end of the DAC chip is connected to the input end of the single-pole double-throw switch circuit, and the control end of the single-pole double-throw switch circuit is connected to the IO port of the controller.

2. The cell electrostimulation system according to claim 1, wherein The pulse generation device has a plurality of channels. Different channels of the pulse generation device correspond to different pulse signal parameters. Among them, the pulse signal parameters at least include the amplitude, frequency, pulse width range of the pulse signal, and the duration of electrical stimulation.

3. The cell electrostimulation system according to claim 2, wherein The system further includes a host computer. The host computer is connected to the pulse generation device and is used to control different channels of the pulse generation device according to the pulse signal parameters to generate corresponding pulse signals and display the current state of the pulse generation device.

4. The cell electrostimulation system according to claim 1, wherein The amplitude of the pulse signal generated by the pulse generation device is greater than 0 and less than 16V, the frequency range is greater than 0.1Hz and less than 500kHz, and the pulse width range is greater than 1μs and less than 4s.

5. A cell electrostimulation system according to claim 1, wherein The system further includes a PCB base, and a plurality of through holes are provided on the PCB base.

6. The cell electrostimulation system according to claim 5, wherein The substrate is fixed on the PCB base, and the graphene array electrode is connected to the PCB base through conductive silver paste.

7. A cell electrostimulation device, characterized in that, The cell electrical stimulation device includes a cell electrical stimulation system as described in claim 1.

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