Reaction vessel mixing device, cleaning and measuring device, and in vitro diagnostic analyzer

Abstract

The present invention discloses a reaction vessel mixing device and its application, comprising: an eccentric wheel, a mounting shell and an oscillation seat, wherein a driving mechanism drives the eccentric wheel to rotate; the mounting shell has a limiting frame, and one end of the oscillation seat has a central hole for accommodating the eccentric wheel; the other end of the oscillation seat passes through the limiting frame and has a clamping groove, and the reaction vessel is inserted into the clamping groove and has a gap with the side wall of the clamping groove. The eccentric wheel rotates in the central hole and can drive the oscillation seat to reciprocate in the limiting frame. When the oscillation seat reciprocates, the side walls on both sides of the clamping groove can impact the reaction vessel. The device has a clever and compact structure and good oscillation effect.

Description

Technical Field

[0001] The present invention relates to the in vitro diagnosis industry, and specifically relates to a reaction vessel mixing device, a cleaning and measuring device, and an in vitro diagnostic analyzer. Background Art

[0002] In sample analysis instruments, a mixing device is often required to mix reaction cups at each operation position. The existing mixing of sample reagents is mostly contact mixing, such as the stirring rod method. The contact mixing method may cause a risk of cross-contamination, or an independent mixing mechanism is set up. After the mixing mechanism mixes, it is transferred to the next operation position by a manipulator. The additional mixing mechanism will occupy space. Summary of the Invention

[0003] The purpose of the present invention is to provide a reaction vessel mixing device with a clever structure, compactness, and good oscillation effect, which is suitable for mixing reaction vessels placed in a card slot in various in vitro diagnostic instruments.

[0004] The technical solution adopted by the present invention is as follows: A reaction vessel mixing device includes:

[0005] An eccentric wheel;

[0006] A driving mechanism, the driving mechanism drives the eccentric wheel to rotate;

[0007] An installation shell, the installation shell and the bottom plate to which the mixing device is to be installed form a limiting frame;

[0008] An oscillation seat, one end of the oscillation seat has a central hole for accommodating the eccentric wheel; the other end of the oscillation seat passes through the limiting frame and has a card slot. The reaction vessel is inserted into the card slot and has a gap between the reaction vessel and the side wall of the card slot. The eccentric wheel rotates in the central hole to drive the oscillation seat to reciprocate in the limiting frame. When the oscillation seat reciprocates, the side walls on both sides of the card slot can impact the reaction vessel.

[0009] Furthermore, the mixing device further includes an optocoupler and a detection optocoupler. The detection optocoupler is connected to the end wall of the oscillation seat with the central hole. The detection optocoupler detects whether the oscillation seat impacts the reaction vessel according to whether the optocoupler returns to the detection optocoupler.

[0010] Furthermore, the reaction vessel is a reaction cup.

[0011] The present invention also provides a cleaning and measuring device, including the above-mentioned reactor mixing device.

[0012] Furthermore, the cleaning and measuring device includes:

[0013] A transfer tray is used to transfer reaction cups to corresponding operating positions, and the operating positions are distributed circumferentially around the transfer tray.

[0014] The reaction vessel mixing device is used to mix the reaction cups in the operating positions; the lower part of the reaction cup in the operating position is inserted into the card slot, and there is a gap between the reaction cup and the side wall of the card slot.

[0015] The reaction vessel mixing device is applicable to mixing reaction cups in any operating position.

[0016] The present invention also provides an in vitro diagnostic analyzer, which includes the above-mentioned mixing device or the above-mentioned cleaning and measuring device.

[0017] The beneficial effects of the present invention: The reaction vessel mixing device of the present invention has a clever and compact structure, good oscillation effect, and is applicable to mixing reaction vessels placed in the card slot in various in vitro diagnostic instruments. Description of the Drawings

[0018] In order to more clearly illustrate the technical solutions of the present invention, the drawings required for use in the embodiments will be briefly introduced below. Obviously, for those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative efforts.

[0019] Figure 1 It is a schematic structural diagram of the mixing mechanism;

[0020] Figure 2 It is a sectional view of the mixing mechanism;

[0021] Figure 3 It is a bottom view of the mixing mechanism;

[0022] Figure 4 It is a schematic structural diagram of the completely shielded structure of the reaction cup pick-up and placement opening of the cleaning and measuring device;

[0023] Figure 5 It is a schematic structural diagram of the completely open structure of the reaction cup pick-up and placement opening of the cleaning and measuring device;

[0024] Figure 6 It is a sectional view of the cleaning and measuring device;

[0025] Figure 7 It is a schematic structural diagram of the light-shielding device;

[0026] 1 - Bottom plate; 2 - Outer housing; 3 - Transfer tray; 4 - Detection mechanism; 5 - Reaction cup pick - up and placement port; 6 - Light - shielding device; 7 - Liquid injection mechanism; 8 - Liquid discharge mechanism; 9 - Mixing device; 10 - Reaction cup; 11 - Acid - adding mechanism; 12 - Alkali - adding mechanism; 101 - Lifting motor; 102 - Lead screw; 103 - Lifting plate; 104 - Guide mechanism; 201 - Base; 202 - Top cover; 203 - Driving motor; 204 - Rolling bearing; 61 - Light - shielding sheet; 62 - Driving unit; 63 - Code disk; 64 - Detection optocoupler 1; 901 - Eccentric wheel; 902 - Driving mechanism; 903 - Mounting shell; 904 - Oscillation base; 905 - Mounting hole; 906 - Card slot; 907 - Optocoupler sheet; 908 - Detection optocoupler 2. Detailed implementation manners

[0027] To make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with specific embodiments of the present invention and the corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention. The following will describe in detail the technical solutions provided by each embodiment of the present invention with reference to the drawings.

[0028] Please refer to Figure 1-3 , a mixing device 9 for a reaction vessel includes: an eccentric wheel 901, a driving mechanism 902, a mounting shell 903 and an oscillation base 904.

[0029] The function of the driving mechanism 902 is to drive the eccentric wheel 901 to rotate. The specific structure is not limited. In this embodiment, the driving mechanism 902 is a driving motor, and the driving motor shaft is connected to the eccentric wheel 901.

[0030] The mounting shell 903 is composed of upper and lower shells, forming a limiting frame. The specific structure is not limited. Its main function is to limit the oscillation base. As long as it can limit the oscillation base up, down, front and back, and at the same time facilitate the installation of the mixing mechanism.

[0031] One end of the oscillation base 904 has a mounting hole 905 for accommodating the eccentric wheel 901; the other end of the oscillation base 904 passes through the limiting frame and has a card slot 906. The lower part of the reaction vessel is inserted into the card slot 906 and there is a gap between the reaction vessel and the side wall of the card slot 906. When the eccentric wheel 901 rotates in the mounting hole 905, it can drive the oscillation base 904 to reciprocate in the limiting frame 906. When the oscillation base 904 reciprocates, the side walls on both sides of the card slot 906 can impact the reaction vessel. The specific shape of the above - mentioned reaction vessel is not limited and can be a test tube or a reaction cup.

[0032] In the embodiments provided by the present invention, the oscillation seat 904 passes through the limiting frame. One end with the mounting hole 905 is located inside the mounting shell 906, and the other end with the card slot 906 extends out of the mounting shell 903. The outer dimension of the middle section of the oscillation seat is the same as that of the limiting frame, which limits the up, down, front and back movement of the oscillation seat 904 and only allows the oscillation seat 904 to move reciprocally left and right.

[0033] Further, in order to more stably realize the reciprocating movement of the oscillation seat 904, in this embodiment, the mounting hole 905 is arranged at the center of the end of the oscillation seat 904. The shape of the mounting hole 905 is waist-shaped, and the width of the waist-shaped mounting hole is the same as the length of the diameter of the eccentric wheel 901. The width of the card slot is not greater than twice the distance from the rotation point of the eccentric wheel to the center point.

[0034] During operation, when the eccentric wheel 901 drives the oscillation seat 904 to move rightward in the limiting frame 906, the left side wall of the card slot 906 can hit the reaction cup. When the eccentric wheel drives the oscillation seat 904 to move leftward in the limiting frame 906, the right side wall of the card slot 906 can hit the reaction cup, so as to form a turbulent flow in the liquid in the reaction cup and promote the reaction of the liquid in the reaction cup.

[0035] The above reaction vessel mixing device further includes an optocoupler 907 and a detection optocoupler two 908. The detection optocoupler two 908 is connected to the end wall of the oscillation seat 904 with the mounting hole. The detection optocoupler two 908 detects whether the oscillation seat 904 hits the reaction cup according to whether the optocoupler 907 is reset to the detection optocoupler two 908.

[0036] Embodiment 2

[0037] A cleaning and measuring device, as Figure 4-7 shown, includes a magnetic bead cleaning and separating device. The magnetic bead cleaning and separating device is an existing structure, including a bottom plate 1, an outer shell 2; a transfer tray 3, a magnetic member, a liquid injection mechanism 7 and a liquid discharge mechanism 8.

[0038] The outer shell 2 is installed on the bottom plate 1. The outer shell 2 is made of light-impermeable material. In this embodiment, as Figure 3 shown, the outer shell includes a base 201 arranged on the bottom plate and a top cover 202 covering the base. The upper part of the base has a hollow cavity. The transfer tray 3 is arranged in the hollow cavity of the base 201. One end of the rotating shaft passes through the base and is connected to the gear disk 203. The gear disk 203 is connected to the motor through a belt, and the other end passes through the base 201 and is connected to the transfer tray 3. The rotating shaft and the base 201 are connected through a rolling bearing 204. The transfer tray has a plurality of reaction cup placement holes; when the motor rotates, the base remains stationary, and the motor drives the transfer tray 3 to transfer the reaction cups to the corresponding operation positions.

[0039] The function of the transfer tray 3 is to transfer the reaction cups to the corresponding operating positions, and the operating positions are distributed circumferentially on the transfer tray, including at least a reaction cup picking and placing position and a cleaning position; the cleaning position includes a liquid injection position and a liquid discharge position.

[0040] A magnetic part is arranged on the side of the transfer path of the transfer tray 3; specifically, the magnetic part is arranged on the inner or outer side of the transfer path of the transfer tray according to the cleaning effect, so as to enable the magnetic beads of the reaction cups to gather on one side of the magnetic part at the corresponding operating positions respectively, so as to achieve sufficient dispersion and effective cleaning of the magnetic beads during the transfer process of the reaction cups.

[0041] A liquid injection mechanism 7 is correspondingly arranged at the liquid injection position for injecting cleaning liquid into the reaction cups at the liquid injection position;

[0042] A liquid discharge mechanism 8 is correspondingly arranged at the liquid discharge position for discharging the liquid in the reaction cups at the liquid discharge position.

[0043] Specifically, the cleaning position includes three liquid injection positions and three liquid discharge positions. The three liquid injection positions and the three liquid discharge positions are alternately distributed between the reaction cup picking and placing position and the acid adding position along the circumferential direction of the transfer tray. The liquid injection mechanism 7 includes three liquid injection needles, and the three liquid injection needles are respectively arranged corresponding to the three liquid injection positions. The liquid discharge mechanism 8 includes three liquid discharge needles, and the three liquid discharge needles are respectively arranged corresponding to the liquid discharge positions.

[0044] The liquid injection mechanism 7 is fixed at the liquid injection position of the top cover. The liquid discharge mechanism 8 is driven by a lifting mechanism to vertically lift to discharge the liquid in the reaction cups at the liquid discharge position; the lifting mechanism is installed above the transfer tray and includes a lifting motor 101, a lead screw 102 and a lifting plate 103. The liquid discharge needles are all fixed on the lifting plate. The upper end of the lead screw 102 is connected to the lifting motor 101, and the lifting motor 101 drives the lead screw 102 to rotate, thereby realizing the vertical lifting movement of the liquid injection needles. Further, the lifting mechanism 10 further includes two guiding mechanisms 104 arranged symmetrically, so as to more stably realize the lifting of the lifting plate. The guiding mechanism 10 includes a guiding shaft and a guiding shaft sleeve. The lower part of the guiding shaft is fixed to the top cover, and the upper part is fixed to the lifting plate through the guiding shaft sleeve.

[0045] In this embodiment, the magnetic bead cleaning, separation and measurement are integrated into one, and specifically further includes an acid adding mechanism 11, an alkali adding mechanism 12, a detection mechanism 4 and the above-mentioned reactor mixing device 9.

[0046] The acid adding mechanism 11 is correspondingly arranged at the acid adding position. The specific structure is not limited as long as it can inject acidic excitation liquid into the reaction cups at the acid adding position. The acid adding mechanism 11 is fixed at the acid adding position of the top cover.

[0047] An alkali adding mechanism 12 is correspondingly arranged at the alkali adding position. The specific structure is not limited as long as it can inject an alkaline excitation liquid into the reaction cup at the alkali adding position. The alkali adding mechanism 12 is fixed at the alkali adding position of the top cover.

[0048] A detection mechanism 4 is correspondingly arranged at the light measuring position. It can be an existing detection mechanism for detecting the light emission intensity of the reaction cup in the light measuring position. A reaction cup pick-up and placement port 5 is also arranged at the position of the top wall of the outer housing 2 corresponding to the reaction cup pick-up and placement position; a light measuring through hole is formed in the side wall of the outer housing, and the detection mechanism 4 is installed at the light measuring through hole and detects the light emission intensity of the reaction cup in the light measuring position through the light measuring through hole.

[0049] The above-mentioned reactor mixing device 9 is correspondingly arranged at the acid adding position for mixing the reaction cup in the acid adding position. Specifically, the lower part of the reaction cup 10 at the acid adding position is inserted into the card slot 906 and has a gap with the side wall of the card slot 906.

[0050] Working process: The reaction cup 10 is placed in the reaction cup pick-up and placement position of the transfer tray 3 through the reaction cup pick-up and placement port 5. As the transfer tray 3 rotates to the liquid injection position, the liquid injection needle injects cleaning liquid into the reaction cup to realize the cleaning of the reaction cup; the reaction cup 10 continues to rotate with the transfer tray 3 to the liquid discharge position, and the liquid discharge needle moves vertically downward and inserts into the reaction cup to suck away the liquid in the reaction cup; one liquid injection and one liquid suction is one cleaning; the liquid injection and liquid suction are repeated three times to realize three cleanings; after three cleanings, the reaction cup rotates with the transfer tray 3 to the acid adding position, and an acidic excitation liquid is injected into the reaction cup. At this time, the reactor mixing mechanism disperses the magnetic beads gathered on one side of the reaction cup to make them fully contact and react with the acidic excitation liquid. The reaction cup rotates with the transfer tray 3 to the alkali adding position, and then an alkaline excitation liquid is injected into the reaction cup, and the detection mechanism starts to measure the light.

[0051] In order to measure more accurately, a completely enclosed whole is formed to avoid external light entering the light measuring position to the greatest extent and ensure the accuracy of the measurement result.

[0052] In another embodiment provided by the present invention, the cleaning and measuring device further includes:

[0053] A light shielding device 6 is correspondingly arranged at the reaction cup pick-up and placement port and can completely shield or open the reaction cup pick-up and placement port. The reaction cup pick-up and placement port 5 is located at the position of the top wall of the outer housing 2 corresponding to the reaction cup pick-up and placement position.

[0054] Specifically, the light shielding device 6 includes: a light shielding sheet 61, a driving unit 62, a code disk 63, and a detection optocoupler 64.

[0055] The light-shielding sheet 61 rotates to completely shield or open the reaction cup access opening; the specific shape of the light-shielding sheet 61 is not limited and can be square, triangular or fan-shaped. In this embodiment, as Figure 1 shown, the shape of the light-shielding sheet is fan-shaped.

[0056] The driving unit 62 drives the light-shielding sheet 61 to rotate. The specific structure of the driving unit 62 is not limited as long as it can drive the light-shielding sheet 61 to rotate forward and backward, and it can be an existing stepping motor.

[0057] The code disk 63 is driven by the driving unit 62 and rotates synchronously with the light-shielding sheet 61. A notch 65 is provided on the outer edge of the code disk; both the code disk 63 and the light-shielding sheet 61 are fixed on the stepping motor rotating shaft.

[0058] The first detection optocoupler 64 is arranged on the outer edge of the code disk 63. The first detection optocoupler 64 detects the rotation angle of the code disk 63, that is, the rotation angle of the stepping motor, according to whether the code disk is reset to the position of the first detection optocoupler. When the first detection optocoupler 64 is blocked by the side edge of the notch 65, it indicates that the code disk is successfully reset. When the detection optocoupler is blocked by the side edge of the notch, the light-shielding sheet can be in a state of completely shielding or opening the reaction cup access opening; in this embodiment, when the first detection optocoupler 64 is blocked by the side edge of the notch 65, it indicates that the code disk is successfully reset. At this time, the light-shielding sheet 61 completely opens the reaction cup access opening 5; when measurement is required, in order to form a completely enclosed measurement environment, the stepping motor rotates a preset angle, the light-shielding sheet 61 completely shields the reaction cup access opening 5, and after the measurement is completed, the stepping motor rotates in the reverse direction by the same angle, and the light-shielding sheet 61 completely opens the reaction cup access opening 5.

[0059] Embodiment 3

[0060] An in vitro diagnostic analyzer includes the above-mentioned measurement and cleaning device.

[0061] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A mixing device for a reaction vessel, characterized in that, Comprising: Eccentric wheel; Driving mechanism for driving the eccentric wheel to rotate; Mounting shell having a limiting frame; Oscillation seat, one end of the oscillation seat having a mounting hole for accommodating the eccentric wheel; the other end of the oscillation seat passes through the limiting frame and has a clamping groove, and the reaction vessel is inserted into the clamping groove and has a gap with the side wall of the clamping groove. The rotation of the eccentric wheel in the mounting hole can drive the oscillation seat to reciprocate in the limiting frame. When the oscillation seat reciprocates, the side walls on both sides of the clamping groove can impact the reaction vessel; The mounting hole is arranged at the center of the end of the oscillation seat, and the shape of the mounting hole is waist-shaped. The width of the waist-shaped mounting hole is the same as the length of the diameter of the eccentric wheel, and the width of the clamping groove is not greater than twice the distance from the rotation point to the center point of the eccentric wheel.

2. The reaction vessel mixing device according to claim 1, wherein The mixing device further includes an optocoupler sheet and a detection optocoupler. The detection optocoupler is connected to the end wall of the oscillation seat having the mounting hole. The detection optocoupler detects whether the oscillation seat impacts the reaction vessel according to whether the optocoupler sheet is reset to the position of the detection optocoupler.

3. The reaction vessel mixing device according to claim 1, wherein The reaction vessel is a reaction cup.

4. A cleaning and measuring device, characterized in that, Comprising: The reactor mixing device according to any one of claims 1-3.

5. The cleaning and measuring device according to claim 4, characterized in that Comprising: Transfer disk for transferring the reaction cup to the corresponding operation position, and the operation positions are distributed circumferentially on the transfer disk; The reaction vessel mixing device for mixing the reaction cup in the operation position; the reaction cup in the operation position is inserted into the clamping groove and has a gap with the side wall of the clamping groove.

6. An in vitro diagnostic analyzer, characterized in that, Comprising the mixing device according to any one of claims 1-3 or the cleaning and measuring device according to claim 4.

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