Pull-out type temperature control device and PCR nucleic acid detector

Abstract

The present invention provides a pull-out temperature control device and a PCR nucleic acid detector, comprising: a housing; a temperature control module slidably disposed in the housing; a self-locking mechanism, a vertical guide member is disposed in the housing, a locking rod is slidably disposed in the vertical guide member in a resetable manner, a locking hook is disposed on the temperature control module and is in clamping cooperation with the bottom end of the locking rod when the temperature control module is pushed into the housing; an unlocking mechanism, the unlocking mechanism includes a swing rod and an unlocking button, the rod body of the swing rod is hinged to the housing, the first end of the swing rod is movably connected to the locking rod to drive the locking rod, and the unlocking button is disposed on the housing and abuts downward against the second end of the swing rod; a pop-up mechanism, the pop-up mechanism is connected to both the temperature control module and the housing to pop the temperature control module out of the housing when the locking rod and the locking hook are unlocked. The present invention can conveniently lock the temperature control module in the housing or pop the temperature control module out of the housing.

Description

Technical Field

[0001] The present invention relates to nucleic acid detection technology, and particularly to a pull-type temperature rising and falling device and a PCR nucleic acid detector. Background Art

[0002] Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication outside the organism. The biggest feature of PCR is that it can greatly increase trace amounts of DNA.

[0003] Real-time fluorescence quantitative PCR technology is a method that adds a fluorescent group to the PCR reaction system, uses the accumulation of fluorescence signals to monitor the entire PCR process in real time, and finally performs quantitative analysis on the unknown template through a standard curve. Real-time fluorescence quantitative PCR technology effectively solves the limitation of traditional quantification that can only be detected at the end point, realizes the detection of the intensity of fluorescence signals once per cycle, records it in the software, and obtains the quantitative result according to the standard curve by calculating the Ct value of each sample. Among them, the Ct value (Cycle threshold) refers to the number of cycles experienced when the fluorescence signal in each reaction tube reaches the set threshold.

[0004] PCR instruments usually include an optical excitation unit and a fluorescence collection unit. During the detection process, the optical excitation unit generates excitation light and directly irradiates the sample through an LED lens. The fluorescence signal generated after the sample is excited reaches the fluorescence collection unit (such as a CCD camera) through a filter and a lens, and the software captures and processes the image for analysis. Currently, the excitation of the sample is generally achieved through thermoelectric heating, through a rotating platform based on convective heating, or an embedded resistance heater, and the required reaction liquid volume is only a few microliters. However, these heating methods cannot achieve extremely fast temperature rising and falling, which brings inconvenience to the detection.

[0005] Therefore, there is a need for a PCR nucleic acid detector that is convenient for detection and can meet extremely fast temperature rising and falling. Summary of the Invention

[0006] In view of the above-mentioned disadvantages of the prior art, the technical problem to be solved by the present invention is to provide a pull-type temperature rising and falling device and a PCR nucleic acid detector, which can conveniently lock the temperature rising and falling module in the casing or eject the temperature rising and falling module out of the casing.

[0007] To solve the above technical problem, the present invention provides a pull-type temperature rising and falling device, comprising:

[0008] A casing;

[0009] A temperature rising and falling module, which is slidably arranged in the casing;

[0010] Self-locking mechanism, the self-locking mechanism includes a vertical guide member, a locking rod and a locking hook. The vertical guide member is provided on the housing. The locking rod is slidably arranged on the vertical guide member in a resetable manner. The locking hook is provided on the temperature rising and falling module and is engaged with the bottom end of the locking rod when the temperature rising and falling module is pushed into the housing.

[0011] Unlocking mechanism, the unlocking mechanism includes a swing rod and an unlocking button. The rod body of the swing rod is hinged to the housing. The two ends in the length direction of the swing rod are respectively a first end and a second end. The first end of the swing rod is movably connected to the locking rod to drive the locking rod. The unlocking button is provided on the housing and abuts downward against the second end of the swing rod.

[0012] Ejecting mechanism, the ejecting mechanism is connected to both the temperature rising and falling module and the housing so that the temperature rising and falling module pops out of the housing when the locking rod and the locking hook are unlocked.

[0013] Preferably, the locking hook includes a wedge-shaped locking block. The small head of the wedge-shaped locking block is hinged to the temperature rising and falling module. The large head of the wedge-shaped locking block protrudes from the temperature rising and falling module in a resetable manner and faces the ejecting direction of the temperature rising and falling module.

[0014] Preferably, the bottom end of the locking rod has a drag-reducing bevel structure.

[0015] Preferably, the side wall of the locking rod has a laterally extending sliding fit groove. The first end of the swing rod has a cylindrical head, and the cylindrical head is slidably arranged in the sliding fit groove.

[0016] Preferably, two plates are symmetrically arranged on the side wall of the locking rod. The distance between the two plates is adapted to the diameter of the cylindrical head. The space defined by the two plates together constitutes the sliding fit groove.

[0017] Preferably, the unlocking button includes a pressing button and a push rod. The pressing button is resetably arranged on the housing. The top end of the push rod is fixedly connected to the pressing button and the bottom end of the push rod abuts against the second end of the swing rod.

[0018] Preferably, the unlocking button further includes a constraint plate. The constraint plate is provided on the housing and is provided with a positioning hole for the push rod to pass through.

[0019] Preferably, the ejecting mechanism includes a guide rod, a pushed plate and a compression elastic member. The end of the guide rod is connected to the housing and the extending direction of the guide rod is parallel to the ejecting direction of the temperature rising and falling module. The pushed plate is provided on the temperature rising and falling module and is provided with an avoidance hole allowing the guide rod to pass through. The compression elastic member is sleeved on the guide rod and the two ends of the compression elastic member in the compression direction respectively abut against the housing and the pushed plate.

[0020] Preferably, the ejecting mechanism further includes an ejecting limit rod and a buffer block. The ejecting limit rod is provided on the housing. The buffer block is provided on the side wall of the ejecting limit rod facing the pushed plate.

[0021] The present invention also provides a PCR nucleic acid detector, comprising the pull-out type temperature rising and falling device.

[0022] As described above, the pull-out type temperature rise and fall device and PCR nucleic acid detector of the present invention have the following beneficial effects: first, the self-locking mechanism includes a vertical guide, a locking rod and a locking hook, the vertical guide is arranged on the housing, the locking rod is resettably slidably arranged on the vertical guide, and the locking hook is arranged on the temperature rise and fall module; when the temperature rise and fall module is pushed into the housing, the locking hook and the bottom end of the locking rod are mutually engaged, thereby locking the temperature rise and fall module in the housing. Furthermore, the unlocking mechanism includes a swing rod and an unlocking button, the shaft of the swing rod is hinged to the housing, the first end of the swing rod is movably connected to the locking rod, and the unlocking button is arranged in the housing and abuts against the second end of the swing rod downward; when the unlocking button is pressed, the unlocking button presses downward on the second end of the swing rod, and the swing rod swings under the drive of the unlocking button, that is, the second end of the swing rod falls, and the first end of the swing rod tilts upward, thereby causing the locking rod to slide upward, thereby causing the bottom end of the locking rod to slip off the locking hook, and the locking relationship between the locking rod and the locking hook is released. At the same time, since the pop-up mechanism is connected to the temperature-raising and cooling module and the housing, the pop-up mechanism enables the temperature-raising and cooling module to pop out of the housing when the locking rod and the lock hook are unlocked. Finally, the unlocking button is released, the locking rod slides down to the original position, the first end of the swing rod falls, and the second end of the swing rod rises. Therefore, the pull-out type temperature-raising and cooling device PCR nucleic acid detector of the present invention can conveniently lock the temperature-raising and cooling module in the housing or pop the temperature-raising and cooling module out of the housing. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 Shown is a first schematic diagram of the PCR nucleic acid detector of the present invention;

[0024] Figure 2 Shown is a second schematic diagram of the PCR nucleic acid detector of the present invention;

[0025] Figure 3 It shows a schematic diagram of the connection structure of the pull-out type temperature raising and lowering device of the present invention;

[0026] Figure 4 Display as Figure 3 A magnified view of part A;

[0027] Figure 5 Display as Figure 3 A magnified view of part B;

[0028] Figure 6 Display as Figure 3 Enlarged view of part C;

[0029] Figure 7 Shown is a diagram of the pull-out state of the PCR nucleic acid detector of the present invention.

[0030] Description of Component Labels

[0031] 1. Housing

[0032] 2. Temperature Rise and Fall Module

[0033] 3. Self-locking Mechanism

[0034] 31. Vertical Guide

[0035] 32. Locking Rod

[0036] 322. Sliding Fit Groove

[0037] 321. Drag Reduction Chamfer Structure

[0038] 33. Lock Hook

[0039] 331. Wedge-shaped Lock Block

[0040] 34. Plate

[0041] 4. Unlocking Mechanism

[0042] 41. Swing Rod

[0043] 411. Cylindrical Head

[0044] 42. Unlocking Button

[0045] 421. Press Button

[0046] 422. Push Rod

[0047] 423. Constraint Plate

[0048] 5. Ejection Mechanism

[0049] 51. Ejection Limit Rod

[0050] 52. Guide Rod

[0051] 53. Pushed Plate

[0052] 54. Compression Elastic Part

[0053] 55. Buffer Block

[0054] 6. Sliding Fit Assembly

[0055] 61. Drawer Track

[0056] 62. Slide Detailed Implementation Modes

[0057] The following specific embodiments illustrate the implementation modes of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification.

[0058] It should be noted that the structures, proportions, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification for those familiar with this technology to understand and read, and are not used to limit the conditions under which the present invention can be implemented. Therefore, they do not have substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size, without affecting the effects that the present invention can produce and the purposes that can be achieved, should still fall within the scope covered by the technical content disclosed in the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle", and "one" cited in this specification are only for the convenience of clear narration and are not used to limit the scope of implementation of the present invention. The change or adjustment of their relative relationship, without substantial change in the technical content, should also be regarded as the scope within which the present invention can be implemented.

[0059] As Figures 1 to 7 shown, the present invention provides a PCR nucleic acid detector, which includes a housing 1 and the following components disposed within the housing 1: a temperature rising and falling module 2, the temperature rising and falling area in the temperature rising and falling module 2 constitutes a stage for placing the PCR chip to be tested; an imaging detection component disposed above the stage, the imaging detection component includes a yellow light component, a camera component, and a blue light component. The above-mentioned yellow light component and blue light component are disposed on both sides of the camera component, and the imaging focus of the camera component is located on the stage, and the outgoing light spots of the yellow light component and the blue light component are located on the stage.

[0060] The PCR nucleic acid detector of the present invention uses a semiconductor thermostat in the temperature rising and falling module 2 to heat the PCR chip, and its temperature rising and falling rate is high, and it can complete the temperature rising and falling of 60°C - 90°C within 2 - 3 s; in addition, the imaging detection component is disposed directly above the stage, and this setting facilitates the integration of the light source module (i.e., the yellow light component and the blue light component) and the camera component. Place the sample to be detected on the PCR chip, then place the PCR chip on the stage, control the temperature rising and falling module 2 to raise and lower the temperature. After the sample is excited, the fluorescence signal is generated, and at the same time, the imaging detection component works, and the camera component obtains a photo of the sample, and then the software processes the photo.

[0061] As Figures 1 to 6 shown, the present invention also provides a pull-out type temperature rising and falling device for a PCR nucleic acid detector, including:

[0062] The housing 1;

[0063] The temperature rising and falling module 2, the temperature rising and falling module 2 is slidably disposed in the housing 1;

[0064] A self-locking mechanism 3, which includes a vertical guide 31, a locking rod 32 and a locking hook 33. The vertical guide 31 is provided on the housing 1, the locking rod 32 is resettably slidably provided on the vertical guide 31, and the locking hook 33 is provided on the temperature-raising and cooling module 2 and is engaged with the bottom end of the locking rod 32 when the temperature-raising and cooling module 2 is pushed into the housing 1;

[0065] The unlocking mechanism 4 includes a swinging rod 41 and an unlocking button 42. The rod body of the swinging rod 41 is hinged to the housing 1. The two ends of the swinging rod 41 in the length direction are respectively a first end and a second end. The first end of the swinging rod 41 is movably connected to the locking rod 32 to drive the locking rod 32. The unlocking button 42 is arranged on the housing 1 and abuts downward against the second end of the swinging rod 41.

[0066] The pop-up mechanism 5 is connected to the temperature-adjusting and cooling module 2 and the housing 1 so that the temperature-adjusting and cooling module 2 pops out of the housing 1 when the locking rod 32 and the locking hook 33 are unlocked.

[0067] In the present invention, firstly, the self-locking mechanism 3 includes a vertical guide member 31, a locking rod 32 and a locking hook 33. The vertical guide member 31 is arranged on the casing 1, the locking rod 32 is resettably slidably arranged on the vertical guide member 31, and the locking hook 33 is arranged on the temperature rise and fall module 2; when the temperature rise and fall module 2 is pushed into the casing 1, the locking hook 33 and the bottom end of the locking rod 32 are engaged with each other, thereby locking the temperature rise and fall module 2 in the casing 1. Furthermore, the unlocking mechanism 4 includes a swinging rod 41 and an unlocking button 42. The rod body of the swinging rod 41 is hinged to the housing 1. The first end of the swinging rod 41 is movably connected to the locking rod 32. The unlocking button 42 is arranged on the housing 1 and abuts downward against the second end of the swinging rod 41. When the unlocking button 42 is pressed, the unlocking button 42 applies downward pressure to the second end of the swinging rod 41. The swinging rod 41 swings under the drive of the unlocking button 42, that is, the second end of the swinging rod 41 falls, and the first end of the swinging rod 41 tilts upward, thereby causing the locking rod 32 to slide upward, so that the bottom end of the locking rod 32 slips off the lock hook 33, and the locking rod 32 and the lock hook 33 are released from the clamping relationship. At the same time, since the pop-up mechanism 5 is connected to the temperature rise and fall module 2 and the housing 1, the pop-up mechanism 5 causes the temperature rise and fall module 2 to pop out of the housing 1 when the lock rod 32 and the lock hook 33 are unlocked. Finally, the unlock button 42 is released, the locking rod 32 slides down to the original position, the first end of the swing rod 41 falls down, and the second end of the swing rod 41 rises. Therefore, the pull-out type heating and cooling device of the present invention can conveniently lock the heating and cooling module 2 in the housing 1 or eject the heating and cooling module 2 out of the housing 1.

[0068] The specific structure of the above-mentioned temperature rise and fall module 2 is as follows: the temperature rise and fall module 2 includes a module shell, a heat dissipation module and a semiconductor thermostat (the semiconductor thermostat includes a semiconductor refrigerator, which can be called a TEC chip). The module shell surrounds and defines a ventilation channel, and the through direction of the ventilation channel is parallel to the pulling direction of the temperature rise and fall module. A temperature rise and fall area for arranging the semiconductor thermostat is formed on the top of the module shell.

[0069] like Figure 4 As shown, in order to simplify the structure of the lock hook 33, the lock hook 33 includes a wedge-shaped lock block 331, the small head of the wedge-shaped lock block 331 is hinged to the temperature-raising and cooling module 2, and the large head of the wedge-shaped lock block 331 can be reset to protrude from the temperature-raising and cooling module 2 and face the ejection direction of the temperature-raising and cooling module 2. When the temperature-raising and cooling module 2 is pushed into the housing 1, the wedge-shaped lock block 331 slides with the bottom end of the locking rod 32 while sinking into the temperature-raising and cooling module 2. Once the wedge-shaped lock block 331 passes the bottom end of the locking rod 32, the wedge-shaped lock block 331 protrudes from the temperature-raising and cooling module 2 again and is engaged with the bottom end of the locking rod 32, so as to prevent the temperature-raising and cooling module 2 from being ejected from the housing 1 under the action of the ejection mechanism 5.

[0070] In order to reduce the resistance encountered by the temperature-raising and cooling module 2 when being pushed into the housing 1, the bottom end of the locking rod 32 has a drag-reducing chamfered structure 321. When the temperature-raising and cooling module 2 is pushed into the housing 1, the drag-reducing chamfered structure 321 slides with the wedge-shaped surface of the wedge-shaped locking block 331.

[0071] like Figure 5 As shown, in order to avoid movement interference between the swing rod 41 and the locking rod 32 when driving the above-mentioned locking rod 32, the side wall of the above-mentioned locking rod 32 has a laterally extending sliding fitting groove 322, and the first end of the above-mentioned swing rod 41 has a cylindrical head 411, which is slidably set in the sliding fitting groove 322.

[0072] Furthermore, in order to facilitate the formation of the above-mentioned sliding fitting groove 322, two plates 34 are symmetrically provided on the side wall of the above-mentioned locking rod 32, and the spacing between the two plates 34 is adapted to the diameter of the cylindrical head 411. The space jointly defined by the two plates 34 constitutes the above-mentioned sliding fitting groove 322.

[0073] like Figure 6 As shown, in order to simplify the structure of the unlocking button 42, the unlocking button 42 includes a push button 421 and a push rod 422. The push button 421 is resettably arranged on the housing 1, the top end of the push rod 422 is fixedly connected to the push button 421 and the bottom end of the push rod 422 abuts against the second end of the swing rod 41.

[0074] To improve the accuracy of the up-and-down movement of the above-mentioned ejector rod 422, the above-mentioned unlocking button 42 further includes a constraint plate 423. The constraint plate 423 is provided on the housing 1 and is provided with a positioning hole for the ejector rod 422 to pass through.

[0075] As Figure 2 shown, in order to eject the above-mentioned temperature control module 2 out of the housing 1, the above-mentioned ejection mechanism 5 includes a guide rod 52, a pushed plate 53, and a compression elastic member 54 (such as a spring). The end of the guide rod 52 is connected to the housing 1 and the extending direction of the guide rod 52 is parallel to the ejection direction of the temperature control module 2. The pushed plate 53 is provided on the temperature control module 2 and is provided with an avoidance hole allowing the guide rod 52 to pass through. The compression elastic member 54 is sleeved on the guide rod 52 and the two ends of the compression elastic member 54 in the compression direction are respectively abutted against the housing 1 and the pushed plate 53. When the temperature control module 2 is pushed into the housing 1, the compression elastic member 54 is in an energy storage state. When the temperature control module 2 ejects into and out of the housing 1, the compression elastic member 54 is in an energy release state.

[0076] To limit the ejection stroke of the above-mentioned temperature control module 2, the above-mentioned ejection mechanism 5 further includes an ejection limit rod 51 and a buffer block 55. The ejection limit rod 51 is provided on the housing 1, and the buffer block 55 is provided on the side wall of the ejection limit rod 51 facing the pushed plate 53. The buffer block 55 can weaken the impact force of the pushed plate 53 on the ejection limit rod 51.

[0077] As Figure 3 shown, in order to achieve the sliding fit between the above-mentioned temperature control module 2 and the housing 1, the above-mentioned pull-out type temperature control device further includes a sliding fit assembly 6. The sliding fit assembly 6 includes a pull-out track 61 and a slide bar 62. The pull-out track 61 is provided on the housing 1, and the slide bar 62 is provided on the temperature control module 2.

[0078] In summary, the pull-out type temperature control device and the PCR nucleic acid detector of the present invention can conveniently lock the temperature control module in the housing or eject the temperature control module out of the housing. Therefore, the present invention effectively overcomes various disadvantages in the prior art and has high industrial utilization value.

[0079] The above-mentioned embodiments only illustratively explain the principles and effects of the present invention, rather than limiting the present invention. Any person familiar with this technology can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes completed by those with ordinary knowledge in the technical field without departing from the spirit and technical idea disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A pull-out temperature raising and lowering device, It is characterized in that include: Housing (1); A temperature rise and fall module (2), the temperature rise and fall module (2) being slidably disposed on the casing (1); A self-locking mechanism (3), the self-locking mechanism (3) comprising a vertical guide (31), a locking rod (32) and a locking hook (33), the vertical guide (31) being arranged on the housing (1), the locking rod (32) being resettably slidably arranged on the vertical guide (31), the locking hook (33) being arranged on the temperature-raising and cooling module (2) and being engaged with the bottom end of the locking rod (32) when the temperature-raising and cooling module (2) is pushed into the housing (1), the locking hook (33) comprising a wedge-shaped locking block (331), the small head of the wedge-shaped locking block (331) being hinged to the temperature-raising and cooling module (2), and the large head of the wedge-shaped locking block (331) being resettably protruding from the temperature-raising and cooling module (2) and facing the ejection direction of the temperature-raising and cooling module (2); An unlocking mechanism (4), the unlocking mechanism (4) comprising a swinging rod (41) and an unlocking button (42), the rod body of the swinging rod (41) being hinged to the housing (1), the two ends of the swinging rod (41) in the length direction being respectively a first end and a second end, the first end of the swinging rod (41) being movably connected to the locking rod (32) to drive the locking rod (32), the unlocking button (42) being arranged on the housing (1) and abutting downward against the second end of the swinging rod (41), when the unlocking button (42) is pressed, the unlocking button (42) applies downward pressure to the second end of the swinging rod (41), and the swinging rod (41) swings under the drive of the unlocking button (42), that is, the second end of the swinging rod (41) falls, and the first end of the swinging rod (41) tilts upward, thereby causing the locking rod (32) to slide upward, thereby causing the bottom end of the locking rod (32) to slip off the lock hook (33), and the locking rod (32) and the lock hook (33) are released from the clamping relationship; The ejection mechanism (5) is connected to the temperature-raising and cooling module (2) and the housing (1) so that the temperature-raising and cooling module (2) is ejected from the housing (1) when the locking rod (32) and the locking hook (33) are unlocked; the ejection mechanism (5) comprises a guide rod (52), a pushed plate (53) and a compression elastic member (54); the end of the guide rod (52) is connected to the housing (1) and the extension direction of the guide rod (52) is parallel to the ejection direction of the temperature-raising and cooling module (2); the pushed plate (53) is arranged on the temperature-raising and cooling module (2) and is provided with an avoidance hole for allowing the guide rod (52) to pass through; the compression elastic member (54) is sleeved on the guide rod (52) and the two ends of the compression elastic member (54) in the compression direction are respectively in contact with the housing (1) and the pushed plate (53).

2. The pull-out temperature raising and lowering device according to claim 1, Features: The bottom end of the locking rod (32) has a drag-reducing chamfered structure (321).

3. The pull-out temperature raising and lowering device according to claim 1, Features: The locking rod (32) has a laterally extending sliding matching groove (322) on its side wall, and the first end of the swing rod (41) has a cylindrical head (411), which is slidably disposed in the sliding matching groove (322).

4. The pull-out temperature control device according to claim 3, characterized in that: Two plates (34) are symmetrically provided on the side wall of the locking rod (32). The distance between the two plates (34) is adapted to the diameter of the cylindrical head (411). The space defined by the two plates (34) together constitutes the sliding fit groove (322).

5. The pull-out temperature control device according to claim 1, characterized in that: The unlocking button (42) includes a pressing button (421) and a push rod (422). The pressing button (421) is resetably arranged on the housing (1). The top end of the push rod (422) is fixedly connected to the pressing button (421), and the bottom end of the push rod (422) abuts against the second end of the swing rod (41).

6. The pull-out temperature control device according to claim 5, characterized in that: The unlocking button (42) further includes a constraint plate (423). The constraint plate (423) is arranged on the housing (1) and is provided with a positioning hole for the push rod (422) to pass through.

7. The pull-out temperature control device according to claim 1, characterized in that: The ejection mechanism (5) further includes an ejection limiting rod (51) and a buffer block (55). The ejection limiting rod (51) is arranged on the housing (1), and the buffer block (55) is arranged on the side wall of the ejection limiting rod (51) facing the pushed plate (53).

8. A PCR nucleic acid detector, characterized in that: It includes the pull-out temperature control device according to any one of claims 1 to 7.

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