Material transfer mechanism, test kit, nucleic acid detection equipment, and material transfer method

Abstract

The present invention relates to a material transfer mechanism, a detection box, a nucleic acid detection device and a material transfer method. The material transfer mechanism includes: an elastic membrane area, the elastic membrane area can expand and deform to form a cavity, and the cavity is used to accommodate materials; a material transfer channel, a plurality of the elastic membrane areas are connected to the material transfer channel, and when there is a pressure difference between two of the elastic membrane areas, the material can be transferred through the material transfer channel. The nucleic acid detection device includes the above-mentioned detection box. The detection box includes the above-mentioned material transfer mechanism. The nucleic acid detection device includes the above-mentioned detection box. The material transfer method is to set an elastic membrane area so that any two of the elastic membrane areas are connected, and when there is a pressure difference between any two of the elastic membrane areas, the material is transferred. The material transfer mechanism can realize the transfer of materials, has a relatively simple structure, can reduce the manufacturing difficulty and manufacturing cost to a certain extent, and is relatively convenient to operate.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a material transfer mechanism, a detection box, a nucleic acid detection device and a material transfer method. Background Art

[0002] In many chemical or biological experiments and tests, samples usually need to be processed in multiple steps. For example, when performing nucleic acid testing, the obtained samples need to be subjected to multiple steps such as lysis, binding, cleaning, and elution. In the above-mentioned multi-step process, materials need to be transferred between different chambers to complete each step separately. However, in the related art, the structure of some material transfer is relatively complex, which makes the operation during material transfer more troublesome and the manufacturing cost is therefore higher. Summary of the Invention

[0003] Based on this, the present invention proposes a material transfer mechanism, which can realize the transfer of materials, has a relatively simple structure, can reduce the manufacturing difficulty and manufacturing cost to a certain extent, and is relatively convenient to operate.

[0004] A material transfer mechanism, comprising:

[0005] an elastic membrane area, wherein the elastic membrane area is capable of expanding and deforming to form a cavity, and the cavity is used to accommodate a material;

[0006] A material transfer channel, wherein the plurality of elastic membrane areas are connected to the material transfer channel, and when there is a pressure difference between two elastic membrane areas, the material can be transferred through the material transfer channel.

[0007] In one embodiment, the material transfer mechanism also includes a main body, the elastic membrane area is connected to the bottom of the main body, the material transfer channel is provided on the main body, and the elastic membrane area can stick to the material transfer channel under the action of rebound force to close the material transfer channel.

[0008] In one embodiment, a protrusion is provided in the area where the material transfer channel contacts the elastic membrane area, and the protrusion is pressed against the elastic membrane area.

[0009] In one embodiment, the material transfer mechanism includes a main body, and a groove is provided on the bottom surface of the main body to form the material transfer channel. The elastic membrane area and the groove have at least a partial area overlapping. When the pressure on the side of the elastic membrane area away from the material transfer channel is less than the pressure on the side close to the material transfer channel, the elastic membrane area expands and deforms, and the material transfer channel is opened; when the pressure on the side of the elastic membrane area away from the material transfer channel is not less than the pressure on the side close to the material transfer channel, the material transfer channel is closed.

[0010] In one embodiment, the main body is further provided with a material pool for storing the material, and the main body is further provided with a feed hole, the feed hole is communicated with the material pool, and the elastic membrane area is connected to the feed hole.

[0011] In one embodiment, the material pool is arranged inside the main body, and a packaging film for sealing the material pool is provided on the top surface of the main body.

[0012] In one embodiment, the material transfer mechanism further includes a driving assembly, the driving assembly includes a base, a receiving cavity is provided on the base, the elastic membrane area is connected to the top of the receiving cavity, and the elastic membrane area can expand and deform and fit into the cavity wall of the receiving cavity.

[0013] In one embodiment, an air hole is provided on the base, and the top of the air hole is connected to the accommodating cavity.

[0014] In one embodiment, in the base, the temperatures of the regions corresponding to at least two of the elastic membrane regions are different.

[0015] The detection box includes the above-mentioned material transfer mechanism.

[0016] Nucleic acid detection equipment, including the above-mentioned detection kit.

[0017] The material transfer method comprises setting elastic membrane areas so that any two of the elastic membrane areas are connected, and when there is a pressure difference between the any two elastic membrane areas, the material is transferred.

[0018] The material transfer mechanism described above forms a cavity for accommodating material through the expansion and deformation of an elastic membrane region. Multiple elastic membrane regions are connected by material transfer channels. When a pressure differential exists between two elastic membrane regions, the material is driven by this pressure differential to move from the high-pressure area to the low-pressure area, thereby achieving material transfer. This mechanism only requires the elastic membrane region and the material transfer channels, resulting in a relatively simple structure, reducing costs and manufacturing complexity. Operation simply requires varying the pressure, making it relatively easy to operate.

[0019] The above-mentioned detection box, by applying the above-mentioned material transfer mechanism, has a relatively simple structure, which can reduce costs and manufacturing difficulty. Moreover, during operation, only the pressure magnitude needs to be changed, and the operation is also relatively simple.

[0020] The above-mentioned nucleic acid detection equipment can reduce costs and manufacturing difficulty by applying the above-mentioned material transfer mechanism, and only needs to change the pressure during operation, so the operation is also relatively simple.

[0021] The material transfer method described above connects any two elastic membrane areas. If a pressure difference exists between the two elastic membrane areas, the material will move under the pressure difference, flowing from the high-pressure area to the low-pressure area, thus achieving material transfer. Operation only requires changing the pressure level, making it relatively simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Schematic diagram of the overall structure of a material transfer mechanism in one embodiment of the present invention;

[0023] Figure 2 for Figure 1 Exploded diagram of the material transfer mechanism;

[0024] Figure 3 for Figure 1 Schematic diagram of the structure of the main body;

[0025] Figure 4 for Figure 1 Schematic diagram of the structure of the middle base;

[0026] Figure 5 is a cross-sectional view of a main body of a material transfer mechanism in another embodiment of the present invention;

[0027] Figure 6 Schematic diagram of the overall structure of the detection box in one embodiment of the present invention;

[0028] Figure 7 for Figure 6 A perspective view of the detection box;

[0029] Figure 8 for Figure 6 A perspective view of the exploded structure of the detection box;

[0030] Figure 9 for Figure 6 A cross-sectional view of the detection box;

[0031] Figure 10 for Figure 6 A top view of the first plate of the detection box;

[0032] Figure 11 for Figure 6 A bottom view of the first plate of the detection box;

[0033] Figure 12 for Figure 6 A top view of the second plate of the detection box;

[0034] Figure 13 is an exploded view of a detection box in one embodiment of the present invention;

[0035] Figure 14 for Figure 13Schematic diagram of the structure of the main parts.

[0036] Reference numerals:

[0037] Material transfer mechanism 000, main body 010, base plate 011, feed hole 0111, material transfer channel 0112, first section of material transfer channel 01121, second section of material transfer channel 01122, third section of material transfer channel 01123, material pool 012, protrusion 013, elastic membrane 020, first elastic membrane area 021, second elastic membrane area 022, base 030, first module 031, first accommodating cavity 0311, first air hole 0312, second air hole 0313, second module 032, second accommodating cavity 0321, third air hole 0322, first sealing ring 041, second sealing ring 042;

[0038] Sample pool 100;

[0039] A first reagent pool 210, a second reagent pool 220, a third reagent pool 230, and a fourth reagent pool 240;

[0040] A first cover 310, a second cover 320, a third cover 330, and a fourth cover 340;

[0041] First plate 400, main channel 410, first section 420 of first branch channel, first section 1 area 421 of first branch channel, first section 2 area 422 of first branch channel, first section 3 area 423 of first branch channel, first section 4 area 424 of first branch channel, first section 5 area 425 of first branch channel, first section 430 of second branch channel, first section 1 area 431 of second branch channel, first section 2 area 432 of second branch channel, first section 3 area 433 of second branch channel, first section 4 area 434 of second branch channel, first section 5 area 435 of second branch channel, first section 440 of sample temporary storage channel, first section 1 area 451 of reagent temporary storage channel, first section 2 area 452 of reagent temporary storage channel, first section 3 area 453 of reagent temporary storage channel, first section 4 area 454 of reagent temporary storage channel, second section 460 of first amplification channel, second section 470 of second amplification channel, fifth reagent reservoir 480;

[0042] The second plate 500, the second section 510 of the first branch channel, the first section 1 area 511 of the second section of the first branch channel, the second section 2 area 512 of the second section of the first branch channel, the third area 513 of the second section of the first branch channel, the fourth area 514 of the second section of the first branch channel, the fifth area 515 of the second section of the first branch channel, the second section 520 of the second branch channel, the first area 521 of the second section of the second branch channel, the second section 2 area 522 of the second section of the second branch channel, the third area 523 of the second section of the second branch channel, the fourth area 524 of the second section of the second branch channel, the fifth area 525 of the second section of the second branch channel, the second section 530 of the sample temporary storage channel, the first area 541 of the second section of the reagent temporary storage channel, the second section 2 area 542 of the second section of the reagent temporary storage channel, the third area 543 of the second section of the reagent temporary storage channel, the fourth area 544 of the second section of the reagent temporary storage channel, the first section 550 of the first amplification channel, the third section 560 of the first amplification channel, and the first section 570 of the second amplification channel;

[0043] The third plate 600, the transfer area 610, the first transfer part 611, the first area of ​​the second transfer part 6121, the second area of ​​the second transfer part 6122, the third area of ​​the second transfer part 6123, the fourth area of ​​the second transfer part 6124, the sample temporary storage area 620, the reagent temporary storage area 1 631, the reagent temporary storage area 2 632, the reagent temporary storage area 3 633, the reagent temporary storage area 4 634, and the amplification reaction area 640. DETAILED DESCRIPTION

[0044] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0045] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0046] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0047] In the present invention, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0048] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0049] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

[0050] See Figures 1 to 3 , respectively showing the overall structural diagram of the material transfer mechanism in one embodiment of the present invention, Figure 1 Exploded diagram of the material transfer mechanism, Figure 1Schematic diagram of the structure of the main body. The material transfer mechanism 000 includes multiple elastic membrane areas and a material transfer channel 0112. For example, the embodiment shown in the accompanying drawings includes a first elastic membrane area 021 and a second elastic membrane area 022, both of which are connected to the material transfer channel 0112. Both the first elastic membrane area 021 and the second elastic membrane area 022 are capable of elastic deformation. When they elastically deform and expand in a certain direction, they form a cavity that can be used to accommodate material. When a pressure difference exists between the first elastic membrane area 021 and the second elastic membrane area 022, the material will be driven by this pressure difference to move from the high-pressure area to the low-pressure area, thereby achieving material transfer.

[0051] It should be noted that although the figures only show two elastic membrane areas, this is not a limitation. More elastic membrane areas can be provided and all connected to the material transfer channel 0112. When a pressure difference exists between any two elastic membrane areas, the material will be moved under the drive of the pressure difference.

[0052] In the accompanying drawings, the first elastic membrane region 021 and the second elastic membrane region 022 are integrated and formed by disposing the elastic membrane 020. That is, the first elastic membrane region 021 and the second elastic membrane region 022 are different regions on the elastic membrane 020. This facilitates manufacturing, as only the elastic membrane 020 needs to be connected to the corresponding other components.

[0053] Of course, the first elastic membrane area 021 and the second elastic membrane area 022 may also be provided separately, that is, a plurality of independent elastic membranes may be provided and connected to corresponding other components respectively.

[0054] In some embodiments, the material transfer mechanism 000 comprises a main body 010 and an elastic membrane 020, with a material transfer channel 0112 disposed at the bottom of the main body 010. Specifically, the bottom surface of the main body 010 is provided with an upwardly recessed groove, which forms the material transfer channel 0112. The elastic membrane 020 is tightly attached to the bottom of the main body 010, with portions of the first elastic membrane region 021 and the second elastic membrane region 022 overlapping with the groove. This allows material within the cavity formed by the downward expansion and deformation of the elastic membrane region to enter the material transfer channel 0112 at its top and flow there.

[0055] Specifically, material transfer channel 0112 includes a first material transfer channel section 01121, a second material transfer channel section 01122, and a third material transfer channel section 01123, which are sequentially connected. First material transfer channel section 01121 is located on top of and within first elastic membrane section 021. Third material transfer channel section 01123 is located on top of and within second elastic membrane section 022. Of course, the shape and dimensions of material transfer channel 0112 are not limited to this; other shapes and dimensions that achieve similar functions are also acceptable.

[0056] When the pressure on the side of the elastic membrane area away from material transfer channel 0112 is lower than the pressure on the side of the elastic membrane area closer to material transfer channel 0112, the elastic membrane area expands and deforms downward to form a cavity, and material transfer channel 0112 opens. When the pressure on the side of the elastic membrane area away from material transfer channel 0112 is no lower than the pressure on the side of the elastic membrane area away from material transfer channel 0112, the elastic membrane area presses against material transfer channel 0112, and material transfer channel 0112 closes.

[0057] When the pressure of the first elastic membrane region 021 is lower than the pressure of the second elastic membrane region 022 , the material in the cavity formed by the second elastic membrane region 022 can be transferred to the first elastic membrane region 021 along the material transfer channel 0112 .

[0058] When the pressure of the first elastic membrane region 021 is greater than the pressure of the second elastic membrane region 022 , the material in the cavity formed by the first elastic membrane region 021 can be transferred to the second elastic membrane region 022 along the material transfer channel 0112 .

[0059] Furthermore, the main body 010 is provided with a material pool 012, in which material can be stored. A top cover is also provided on the top of the material pool 012 to seal the material therein. Specifically, the main body 010 includes a base plate 011, with the material pool 012 disposed on top of the base plate 011. A through-hole 0111 is provided on the base plate 011, located at the bottom of the material pool 012. The first elastic membrane region 021 is attached to the bottom of the feed hole 0111. When the first elastic membrane region 021 is downwardly adsorbed and the pressure on the side of the first elastic membrane region 021 away from the material transfer channel 0112 is lower than the pressure on the side closer to the material transfer channel 0112, the first elastic membrane region 021 expands and deforms downward, forming a cavity. Simultaneously, the material in the material pool 012 flows through the feed hole 0111 into the cavity formed by the first elastic membrane region 021, thereby enabling feeding.

[0060] When the material completes its reaction within the cavity formed by the first elastic membrane region 021 and needs to be transferred to the second elastic membrane region 022, the second elastic membrane region 022 is attracted downward, causing the pressure there to be lower than that of the first elastic membrane region 021. Simultaneously, the attraction to the first elastic membrane region 021 is gradually removed, causing it to slowly rebound and return to its original position. During this process, the second elastic membrane region 022 expands and deforms downward to form a cavity, while the cavity formed by the first elastic membrane region 021 gradually shrinks. The material within the cavity formed by the first elastic membrane region 021 is gradually pressed into the cavity formed by the second elastic membrane region 022 through the material transfer channel 0112, thereby achieving material transfer between the different elastic membrane regions.

[0061] Preferably, the material pool 012 can be arranged inside the main body 010, that is, inside the substrate 011. A packaging film is provided on the top surface of the main body 010, and the packaging film is adhered to the top surface of the main body 010 to close the material pool 012. After the material is filled, the film can be quickly affixed to the top surface of the main body 010. Of course, it is also possible to directly use tape to stick it on the top surface of the main body 010. Compared with the method of using a top cover, direct packaging through a film material is more convenient to operate, and there is no need to specially manufacture a top cover of the corresponding shape, which simplifies the manufacturing process and is also lower in cost.

[0062] See Figures 2 to 4 , Figure 4 Shown Figure 1 Schematic diagram of the structure of the middle base. Preferably, a drive assembly is also provided at the bottom of the elastic membrane 020. The drive assembly includes a base 030, which comprises a first module 031 and a second module 032. The first module 031 is provided with a first accommodating cavity 0311, and the second module 032 is provided with a second accommodating cavity 0321. The position of the first accommodating cavity 0311 corresponds to the first elastic membrane area 021, and the position of the second accommodating cavity 0321 corresponds to the second elastic membrane area 022. When the first elastic membrane area 021 expands and deforms downward, it enters the first accommodating cavity 0311 and conforms to the wall of the first accommodating cavity 0311. When the second elastic membrane area 022 expands and deforms downward, it enters the second accommodating cavity 0321 and conforms to the wall of the second accommodating cavity 0321.

[0063] By setting a accommodating cavity at the corresponding position at the bottom of the elastic membrane area, the shape of the elastic membrane when deformed can be limited. After the material enters the cavity formed by the elastic membrane, the cavity wall of the accommodating cavity can provide certain support for it, so that it can better support the material.

[0064] Preferably, first module 031 is provided with a first air hole 0312 and a second air hole 0313, and second module 032 is provided with a third air hole 0322. First air hole 0312 is located at the bottom of feed hole 0111, second air hole 0313 is located at the bottom of first elastic membrane area 021, and third air hole 0322 is located at the bottom of second elastic membrane area 022. The drive assembly also includes components such as an air pump, which can be connected to the bottom of the air holes. By pumping air through the corresponding air holes, the pressure in the accommodating chamber is changed, thereby achieving expansion and deformation of the elastic membrane area.

[0065] Specifically, when the air pump's suction pipe is connected to the bottoms of first and second air holes 0312 and 0313, first elastic membrane region 021 expands and deforms downward, adhering to the wall of first accommodating chamber 0311, separating first elastic membrane region 021 from feed hole 0111. Material within material pool 012 flows downward through feed hole 0111 into the cavity formed by first elastic membrane region 021. When the air pump's suction pipe is connected to third air hole 0322 and the suction force applied to first and second air holes 0312 and 0313 is gradually reduced, second elastic membrane region 022 expands and deforms downward, adhering to the wall of second accommodating chamber 0321. Material within the cavity formed by first elastic membrane region 021 is gradually pressed through material transfer channel 0112 into the cavity formed by second elastic membrane region 022.

[0066] Preferably, a temperature control component is provided in the base 030 for adjusting the temperature of the elastic membrane area, that is, the temperature of the material in the cavity formed by the elastic membrane area can be adjusted.

[0067] Preferably, the temperature of at least two elastic membrane areas in the base 030 is different. For example, the temperature of the first module 031 is different from that of the second module 032. In this way, when the material is transferred between the two elastic membrane areas, the temperature of the material can be quickly increased or decreased.

[0068] Preferably, the elastic membrane 020 can be made of materials such as latex, silicone, or PDMS. When the material transfer mechanism 000 is used in a test kit and nucleic acid detection equipment, the material within the cavity formed by the elastic membrane needs to be heated or cooled during the PCR reaction. After the PCR reaction, fluorescence detection is required. These materials have good elasticity, optical transparency, and thermal conductivity, and can effectively meet the requirements of the test kit.

[0069] Preferably, the main body 010 is made of PP, PC, PMMA, COC, etc. These materials are easy to be injection molded, have no fluorescence, and will not affect the accuracy of fluorescence detection.

[0070] Preferably, the elastic membrane 020 is fixed to the main body 010 and the base 030 by bonding, for example, by UV adhesive, nano adhesive, etc. Of course, if the main body 010 and the base 030 are made of metal, welding can also be used for fixing.

[0071] As previously described, the first elastic membrane region 021 and the second elastic membrane region 022 are distinct regions on the elastic membrane 020. Alternatively, the first elastic membrane region 021 and the second elastic membrane region 022 may be provided separately. In this case, the elastic membrane 020 can be secured to the main body 010 and the base 030 by bonding. If the first elastic membrane region 021 and the second elastic membrane region 022 are provided separately, they can be embedded in another component to secure the component to the main body 010 and the base 030.

[0072] Preferably, sealing rings are provided between the base 030 and the first and second elastic membrane sections 021 and 022. Specifically, a first sealing ring 041 is provided on top of the first module 031, and a second sealing ring 042 is provided on top of the second module 032. This allows the first and second elastic membrane sections 021 and 022 to fit more closely with the accommodating cavity of the base 030, thereby enhancing the sealing between the first and second elastic membrane sections 021 and 022 and the accommodating cavity of the base 030.

[0073] Preferably, when a certain elastic membrane area is not in use, the elastic membrane area is elastically deformed. Under the action of its rebound force, the elastic membrane area tends to rebound upward and return to its original position, tightly adhering to the material transfer channel 0112. Material within the material transfer channel 0112 will not flow into the elastic membrane area. This keeps the material transfer channel 0112 closed, preventing material from shifting unnecessarily during transportation and when the elastic membrane area is not in use.

[0074] See Figure 5 , shows a cross-sectional view of the main body of a material transfer mechanism according to another embodiment of the present invention. In some embodiments, a downwardly projecting protrusion 013 can be provided on the main body 010 in the area where the material transfer channel 0112 contacts the elastic membrane area. Protrusion 013 elastically deforms the elastic membrane area. The elastic membrane area's rebound force causes the elastic membrane area to deform upward and return to its original position. Consequently, the protrusion presses against the material transfer channel 0112, thereby closing it.

[0075] In some embodiments, the base 030 can be used to press the elastic membrane area upward against the material transfer channel 0112, thereby closing the material transfer channel 0112.

[0076] In some embodiments, an air pump in the driving assembly may be used to blow air toward the elastic membrane area, and the air may support the elastic membrane area so that the elastic membrane area is pressed against the material transfer channel 0112 , thereby closing the material transfer channel 0112 .

[0077] In some embodiments, a material transfer method is provided. Specifically, elastic membrane regions are provided to connect any two elastic membrane regions. The elastic membrane regions are capable of deformation. When they deform and expand, they form a cavity to accommodate the material. When a pressure difference exists between any two elastic membrane regions, the material, driven by the pressure difference, moves from the high-pressure area to the low-pressure area, thereby achieving material transfer. Specifically, any two elastic membrane regions can be connected through a channel.

[0078] When there is a pressure difference between any two elastic membrane areas, the pressure of the accommodation cavity corresponding to one of the elastic membrane areas can be made lower than that of the other elastic membrane area by sucking, thereby achieving material suction.

[0079] See Figures 6 to 8 , respectively showing the overall structural diagram of the detection box in one embodiment of the present invention, Figure 6 Perspective view of the detection box, Figure 6 The above-mentioned material transfer mechanism can be applied to the test box to realize nucleic acid detection. The test box includes the material transfer mechanism of any of the above-mentioned embodiments and has the beneficial effects of the material transfer mechanism of any of the above-mentioned embodiments. During nucleic acid detection, there are multiple steps. At each step, samples or reagents need to be transferred. Using the above-mentioned material transfer mechanism 000, the samples or reagents can be transferred smoothly. The following is the specific structure of the test box.

[0080] The detection box provided in one embodiment of the present invention includes at least one sample pool 100, multiple reagent pools, a flow channel assembly, a transfer area 610 and other components. The sample pool 100 is used to store samples, and different reagents can be stored in each reagent pool. The flow channel assembly includes a transfer flow channel, and the sample pool 100 and the transfer area 610 can be connected through the transfer flow channel. When there is a pressure difference between the two ends of the transfer flow channel, the substance can be transferred through the transfer flow channel. Each reagent pool can also be connected to the transfer area 610 through the transfer flow channel. When there is a pressure difference between the two ends of the transfer flow channel, the substance can be transferred through the transfer flow channel.

[0081] Preferably, the detection box also includes a magnetic part. The sample in the sample pool 100 reaches the transfer area 610 through the transfer flow channel. During this process, the magnetic part fixes the effective substance in the sample in the transfer flow channel, and the remaining waste liquid can be returned to the sample pool 100 by the original route. In the process of the reagent in the reagent pool reaching the transfer area 610 through the transfer flow channel, it will come into contact with the effective substance in the transfer flow channel, and the waste liquid after contact can also be returned to the reagent pool by the original route. The reagents in each reagent pool are in contact with the effective substance in the transfer flow channel in turn in the above manner. In this way, multiple steps of processing can be completed in one detection box.

[0082] The detection kit can be used for chemical or biological experimental detection, for example, can be used for nucleic acid detection. In this embodiment, the detection kit is described by taking nucleic acid detection as an example.

[0083] Among them, the sample pool 100 contains cell lysis solution, which is used to lyse samples such as cells and viruses to release nucleic acid substances. The top of the sample pool 100 has a first cover 310, which is locked between the first cover 310 and the sample pool 100. By opening the first cover 310, the extracted sample can be placed therein. There are four reagent pools, namely the first reagent pool 210, the second reagent pool 220, the third reagent pool 230 and the fourth reagent pool 240. A second cover 320 is provided on the top of the first reagent pool 210 and the second reagent pool 220, and a third cover 330 is provided on the top of the third reagent pool 230 and the fourth reagent pool 240. The second cover 320 and the third cover 330 are both non-detachable. The reagents in the reagent pool are sealed by each cover to prevent them from leaking.

[0084] Both the first reagent pool 210 and the second reagent pool 220 contain cleaning fluid for cleaning the cleaved nucleic acids and removing impurities. Magnetic particles are provided in the transfer channel for adsorbing the cleaved nucleic acids. These magnetic particles are fixed by magnetic components, thereby securing the nucleic acids within the transfer channel. The third reagent pool 230 contains an eluent for eluting and separating the nucleic acids adsorbed on the magnetic particles. The fourth reagent pool 240 contains PCR reaction solution 1, which contains various enzymes, primers, and other substances required for nested PCR.

[0085] It should be noted that the positional relationship between the sample reservoir 100 and the various reagent reservoirs is not limited. The accompanying drawings only provide one arrangement, and other arrangements are possible. For example, the second reagent reservoir 220 for the cleaning solution and the third reagent reservoir 230 for the eluent solution can be interchanged. The number of reagent reservoirs and the types of reagents within them are also not limited and can be selected based on experimental needs, such as adding a diluent reservoir or other reagent reservoir.

[0086] When performing nucleic acid testing, the test kit only needs to first introduce the sample into the transfer channel, absorb the lysed nucleic acids onto the magnetic particles within the transfer channel, and then pass the reagents in each reagent pool through the transfer channel in sequence, thus completing multiple processing steps within a single test kit. Furthermore, the entire test kit has a relatively small number of components and a simple structure, which can reduce manufacturing costs to a certain extent and improve its market competitiveness.

[0087] Preferably, the above components are made of plastic, so that the entire detection box is light and low in cost. The components can be integrally injection molded, or they can be separately molded and then fixed by bonding or other methods.

[0088] Preferably, in some embodiments, the transfer area 610 includes a first transfer portion 611 and a plurality of second transfer portions. The transfer channel includes a main channel 410, a plurality of first branch channels, and a plurality of second branch channels. The first end of each first branch channel is connected to the main channel 410, and the first transfer portion 611 and each second transfer portion are connected to the second end of the first branch channel in a one-to-one correspondence. The first end of each second branch channel is connected to the main channel 410, and the sample pool 100 and each reagent pool are connected to the second end of the second branch channel in a one-to-one correspondence.

[0089] Specifically, the plurality of second transfer sections are the second transfer section first area 6121, the second transfer section second area 6122, the second transfer section third area 6123, and the second transfer section fourth area 6124. There are five first branch flow channels, each of which is connected to one of the first transfer section 611, the second transfer section first area 6121, the second transfer section second area 6122, the second transfer section third area 6123, and the second transfer section fourth area 6124. There are five second branch flow channels, each of which is connected to one of the sample reservoir 100, the first reagent reservoir 210, the second reagent reservoir 220, the third reagent reservoir 230, and the fourth reagent reservoir 240.

[0090] The transfer area 610 is divided into multiple parts, and the first branch flow channel and the second branch flow channel are also provided with multiple parts correspondingly, and the multiple first branch flow channels and the multiple second branch flow channels intersect only at the main flow channel 410. Therefore, each of the sample pool 100 and the multiple reagent pools can correspond to a flow path. For example, the sample in the sample pool 100 flows to the main flow channel 410 through one of the second branch flow channels, and flows to one of the second transfer parts through one of the first branch flow channels. The reagent in the first reagent pool 210 flows to the main flow channel 410 through another second branch flow channel, and flows to another second transfer part through another first branch flow channel. In this way, when the sample in the sample pool 100 and the reagent in each reagent pool flow in the transfer flow channel, there is only a path overlap at the main flow channel 410, which can reduce the mutual influence between the various reagents remaining in the flow channel and make the detection results more accurate.

[0091] It should be noted that the shapes of the first branch flow channels and the second branch flow channels are not limited and can be adjusted according to the specific shape and size of the detection box. The number of the first branch flow channels and the second branch flow channels can also be adjusted according to the number of the sample pool 100 and the reagent pool.

[0092] In some embodiments, the magnetic member is an electromagnet. When powered on, the electromagnet is magnetic and the magnetic particles are fixed. When powered off, the electromagnet is non-magnetic and the magnetic particles are free in the transfer channel.

[0093] In some embodiments, the test kit includes a first plate 400 and a second plate 500. The first plate 400 is fixed to the top of the second plate 500. The first plate 400 and the second plate 500 can be manufactured separately and then fixedly connected, or they can be integrally formed. If they are manufactured separately and then connected, a seal can be provided between the two to enhance the seal. The sample pool 100 and each reagent cell are connected to the first plate 400.

[0094] The transfer area 610 is connected to the second plate 500. The first branch channel includes the first branch channel first section 420 and the first branch channel second section 510. The second branch channel includes the second branch channel first section 430 and the second branch channel second section 520. The main channel 410, the first branch channel first section 420, and the second branch channel first section 430 are all arranged on the bottom surface of the first plate 400. The first branch channel second section 510 and the second branch channel second section 520 are both arranged on the second plate 500 and pass through the second plate 500. The first branch channel first section 420 corresponds to the first branch channel second section 510 and is connected thereto. The second branch channel first section 430 corresponds to the second branch channel second section 520 and is connected thereto.

[0095] The substances in the sample pool 100 or each reagent pool can flow through the second branch channel second section 520, the second branch channel first section 430, the main channel 410, the first branch channel first section 420 and the first branch channel second section 510 in sequence, and finally enter the corresponding first transfer part 611 or the second transfer part.

[0096] In this embodiment, since each flow channel is arranged inside the component, it has good airtightness and can isolate the sample or reagent from the outside world to prevent foreign substances from entering, thereby improving the accuracy of the test results. In addition, the structure is simple and easy to manufacture. It only needs to set two plates and hollow out each flow channel on the plates. If it is manufactured in a split manner, it is only necessary to dig out a depression on the bottom surface of the first plate 400 to form each first branch flow channel first section 420 and the second branch flow channel first section 430, and dig out multiple through holes on the second plate 500 to form the first branch flow channel second section 510 and the second branch flow channel second section 520. The shapes of the first branch flow channel first section 420 and the second branch flow channel first section 430 are not limited and can be adjusted according to the specific shape and size of the test box.

[0097] In some embodiments, the test kit further includes a sample storage area 620 and multiple reagent storage areas. The transfer flow path further includes a sample storage channel and multiple reagent storage channels. The sample storage area 620 corresponds to the position of the sample pool 100, and each reagent pool corresponds to a reagent storage area.

[0098] The sample pool 100 and the sample temporary storage area 620 can be connected through the sample temporary storage flow channel. If the pressure of the sample temporary storage area 620 is lower than that of the sample pool 100, the sample can be transferred from the sample pool 100 to the sample temporary storage area 620. The sample temporary storage area 620 is connected to the transfer flow channel. Specifically, the sample temporary storage area 620 is connected to the second section 520 of the second branch channel at the corresponding position. That is, the sample in the sample pool 100 first reaches the sample temporary storage area 620 from the sample pool 100 through the sample temporary storage flow channel, and then flows through the second section 520 of the second branch channel, the first section 430 of the second branch channel, the main channel 410, the first section 420 of the first branch channel, and the second section 510 of the first branch channel in sequence. If the pressure of the sample temporary storage area 620 is higher than that of the sample pool 100, the sample is transferred to the sample pool 100 in the opposite direction of the above path.

[0099] Each reagent cell can be communicated with the reagent temporary storage area of ​​the corresponding position through the corresponding reagent temporary storage flow channel. If the pressure in the reagent temporary storage area is less than that in the reagent cell, the sample can be transferred from the reagent cell to the reagent temporary storage area. Each reagent temporary storage area is connected to the transfer flow channel of the corresponding position. Specifically, each reagent temporary storage area is connected to the second branch flow channel second section 520 of the corresponding position. That is, the reagent in the reagent cell first reaches the reagent temporary storage area from the reagent cell through the reagent temporary storage flow channel, and then flows through the second branch flow channel second section 520, the second branch flow channel first section 430, the main channel 410, the first branch flow channel first section 420 and the first branch flow channel second section 510 in sequence. The waste liquid is recycled in the opposite direction of the above path. If the pressure in the reagent temporary storage area is greater than that in the reagent cell, the reagent is transferred to the reagent cell in the opposite direction of the above path.

[0100] When there is a pressure difference between the sample temporary storage area 620 or the reagent temporary storage area and the transfer area 610 , samples or reagents can be transferred between the sample temporary storage area 620 and the transfer area 610 , or reagents can be transferred between the reagent temporary storage area and the transfer area 610 .

[0101] The detection box is relatively independent when performing various processes. For example, the sample is transferred from the sample pool 100 to the sample temporary storage area 620, and then transferred to a certain area in the transfer area 610. After the nucleic acid is fixed in the transfer flow channel, it is returned to the sample pool 100 along the original route. After that, the reagent in any reagent pool can be made to reach the corresponding reagent temporary storage area and transferred to another area in the transfer area 610 through the transfer flow channel. During this process, the reagent contacts the nucleic acid in the transfer flow channel. Afterwards, the waste liquid is returned to the original reagent pool along the original route. Then, the reagents in other reagent pools are also contacted with the nucleic acid in the above manner. In the above process, when the reagent in one of the reagent pools is involved in the contact, as long as there is no pressure difference between the other reagent pools and the corresponding reagent temporary storage area, it can be ensured that the reagents therein will not flow into the transfer flow channel again. The various processes can be relatively independent and not easily affect each other, which can make the test results more accurate.

[0102] Furthermore, a third plate 600 is provided at the bottom of the second plate 500. A sample temporary storage area 620, a reagent temporary storage area, and a transfer area 610 are provided at corresponding locations on the third plate 600. Specifically, the corresponding locations on the third plate 600 can be hollowed out, and elastic membranes can be fixed to the hollowed-out holes to form the sample temporary storage area 620, the reagent temporary storage area, and the transfer area 610.

[0103] The sample pool 100 and the reagent pool are both arranged on the top of the first plate 400, and the sample temporary storage area 620, the reagent temporary storage area and the transfer area 610 are all located at the bottom of the second plate 500. The aforementioned reagent temporary storage flow channel and the sample temporary storage flow channel run through the first plate 400 and the second plate 500, so that the sample can be transferred between the sample pool 100 and the sample temporary storage area 620, and the reagent can be transferred between the reagent pool and the corresponding reagent temporary storage area.

[0104] In this embodiment, the reagent temporary storage flow channel and the sample temporary storage flow channel may be through holes extending in a vertical direction. Of course, other inclined through holes are also acceptable.

[0105] When the elastic membrane is adsorbed downward, it can deform and expand downward to form a cavity, and then absorb the sample in the sample pool 100. When the elastic membrane in the reagent temporary storage area deforms and expands downward to form a cavity, it can absorb the reagent in the reagent pool. When the adsorption of the elastic membrane is removed, the elastic membrane returns to its original position and fits tightly to the bottom of the second plate 500, and the waste liquid in the cavity can be pressed upward into the sample pool 100 or the reagent pool. Alternatively, when the elastic membrane in the transfer area 610 is adsorbed downward to expand to form a cavity, at the same time, the elastic membrane in the sample temporary storage area 620 or a certain reagent temporary storage area rebounds and returns to its original position, and substances can be transferred between the transfer area 610 and the corresponding sample temporary storage area or reagent temporary storage area.

[0106] A pneumatic part such as a puller or an air pump can be connected to the elastic membrane to achieve deformation, expansion, or rebound and reset of the adsorbed elastic membrane.

[0107] In this structure, once the waste liquid returns, as long as it no longer attracts the elastic membrane and deforms downward, it can be stably stored in the sample reservoir 100 or reagent reservoir without affecting subsequent processing steps. Furthermore, this structure is relatively simple, requiring only the elastic membrane to be fixed to a corresponding position on the bottom of the second plate 500. Elastic membranes are also readily available and relatively inexpensive.

[0108] Preferably, protruding bumps can be provided on the bottom of the second plate 500 at locations corresponding to the respective elastic membranes to elastically deform the elastic membranes. Under the action of their rebound force, the elastic membranes can be stably attached to the bottom of the second plate 500, without affecting subsequent processing steps, further improving the stability of the test cartridge during use. Furthermore, the reagents will not shift during transportation.

[0109] Alternatively, a supporting member may be provided at the bottom of the elastic membrane to press the elastic membrane tightly so that when deformation is not required, the elastic membrane can be stably attached to the bottom of the second plate 500 without affecting other subsequent processing steps, thereby further improving the stability of the detection box during use.

[0110] Alternatively, a base can be provided at the bottom of the elastic membrane in the manner described above, with a cavity provided on the base to accommodate the deformed elastic membrane and provide support therefor. Preferably, a sealing ring can be provided between the elastic membrane and the base to enhance sealing. Preferably, the base is also provided with air holes, through which components such as an air pump can pump and expand the elastic membrane. Furthermore, the aforementioned magnetic member can also be provided on the base.

[0111] See Figures 9 to 12 , Figures 9 to 12Shown respectively Figure 6 Cross-sectional view of the test box, Figure 6 A top view of the first plate of the detection box, Figure 6 Bottom view of the first plate of the detection box, Figure 6 A top view of the second plate of the detection box is shown in FIG.

[0112] Specifically, the aforementioned sample temporary storage area 620 is located directly below the sample reservoir 100, and the multiple reagent temporary storage areas are reagent temporary storage area 1 631, reagent temporary storage area 2 632, reagent temporary storage area 3 633, and reagent temporary storage area 4 634. Reagent temporary storage area 1 631 is located below the first reagent reservoir 210, reagent temporary storage area 2 632 is located below the second reagent reservoir 220, reagent temporary storage area 3 633 is located below the third reagent reservoir 230, and reagent temporary storage area 4 634 is located below the fourth reagent reservoir 240.

[0113] See Figure 8 、 Figure 9 and Figure 11 The sample temporary flow channel includes a first section 440 of the sample temporary flow channel that runs through the first plate 400, and a second section 530 of the sample temporary flow channel that runs through the second plate 500. The bottom end of the first section 440 of the sample temporary flow channel is aligned with the top end of the second section 530 of the sample temporary flow channel, and the two are connected. Both are located within the sample temporary storage area 620. When a pressure difference exists between the sample reservoir 100 and the sample temporary storage area 620, the sample can flow between the sample reservoir 100 and the sample temporary storage area 620 through the first section 440 of the sample temporary flow channel and the second section 530 of the sample temporary flow channel. The third section 523 of the second branch flow channel is located on one side of the second section 530 of the sample temporary flow channel and runs through the second plate 500. The third section 523 of the second branch flow channel is located within the sample temporary storage area 620.

[0114] A first section and area 451 of a reagent temporary storage channel is also provided on the first plate 400, and a second section and area 541 of a reagent temporary storage channel is provided on the second plate 500. The bottom of the first section and area 451 of the reagent temporary storage channel is aligned with the top of the second section and area 541 of the reagent temporary storage channel, and the two are connected. Both are located within the first reagent storage area 631. When a pressure difference exists between the first reagent reservoir 210 and the first reagent storage area 631, the reagent can flow between the first reagent reservoir 210 and the first reagent storage area 631 via the first section and area 451 of the reagent temporary storage channel and the second section and area 541 of the reagent temporary storage channel. The second section and area 521 of the second branch channel is located on one side of the second section and area 541 of the reagent temporary storage channel and extends through the second plate 500. The second section and area 521 of the second branch channel is located within the first reagent storage area 631.

[0115] Similarly, the second reagent tank 220 and the reagent temporary storage area 2 632 are connected via the reagent temporary storage flow channel first section 2 area 452 and the reagent temporary storage flow channel second section 2 area 542 , and a second branch flow channel second section 2 area 522 is provided on one side of the reagent temporary storage flow channel second section 2 area 542 .

[0116] The third reagent tank 230 and the reagent temporary storage area 3 633 are connected through the reagent temporary storage flow channel 1 section 3 area 453 and the reagent temporary storage flow channel 2 section 3 area 543 . A second branch flow channel 2 section 4 area 524 is provided on one side of the reagent temporary storage flow channel 2 section 3 area 543 .

[0117] The fourth reagent tank 240 and the reagent temporary storage area 434 are connected through the reagent temporary storage flow channel first section 4 section 454 and the reagent temporary storage flow channel second section 4 section 544. A second branch flow channel second section 5 section 525 is provided on one side of the reagent temporary storage flow channel second section 4 section 544.

[0118] See Figure 8 and Figure 11 The five second branch channel first sections 430 provided on the bottom surface of the first plate 400 are respectively the first section 1 area 431 of the second branch channel, the second section 2 area 432 of the second branch channel, the third section 433 of the second branch channel, the fourth section 434 of the second branch channel, and the fifth section 435 of the second branch channel. The first section 1 area 431 of the second branch channel is connected to the main channel 410 at one end, and the other end is located at the top of the second section 1 area 521 of the second branch channel on the second plate 500, and the two are connected. The first section 2 area 432 of the second branch channel is connected to the main channel 410 at one end, and the other end is located at the top of the second section 2 area 522 of the second branch channel on the second plate 500, and the two are connected. The first section 3 area 433 of the second branch channel is connected to the main channel 410 at one end, and the other end is located at the top of the second section 3 area 523 of the second branch channel on the second plate 500, and the two are connected. One end of the second branch channel's first section, fourth zone 434 is connected to the main channel 410, and the other end is located at the top of the second branch channel's second section, fourth zone 524 on the second plate 500, connecting the two. One end of the second branch channel's first section, fifth zone 435 is connected to the main channel 410, and the other end is located at the top of the second branch channel's second section, fifth zone 525 on the second plate 500, connecting the two.

[0119] The five first branch channel first sections 420 provided on the bottom surface of the first plate 400 are respectively the first branch channel first section area 1 421, the first branch channel first section area 2 422, the first branch channel first section area 3 423, the first branch channel first section area 424, and the first branch channel first section area 525. The first branch channel first section area 1 421 has one end connected to the main channel 410 and the other end located at the top of the first branch channel second section area 1 511 on the second plate 500, with the two being connected. The first branch channel first section area 2 422 has one end connected to the main channel 410 and the other end located at the top of the first branch channel second section area 2 512 on the second plate 500, with the two being connected. The first branch channel first section area 3 423 has one end connected to the main channel 410 and the other end located at the top of the first branch channel second section area 3 513 on the second plate 500, with the two being connected. One end of the first branch channel's first section, fourth zone 424 is connected to the main channel 410, and the other end is located at the top of the first branch channel's second section, fourth zone 514 on the second plate 500, connecting the two. One end of the first branch channel's first section, fifth zone 425 is connected to the main channel 410, and the other end is located at the top of the first branch channel's second section, fifth zone 515 on the second plate 500, connecting the two.

[0120] See Figures 6 to 12 , each process is described. First, open the first cover 310, place the extracted sample into the sample pool 100, and then cover the first cover 310. The sample is lysed in the sample pool 100 to release nucleic acids. During this process, preferably, a stirrer can be provided to stir it to accelerate lysis. Alternatively, an ultrasonic device can be provided to accelerate lysis by ultrasound. Alternatively, a heating device can be provided to accelerate lysis by increasing the temperature. Alternatively, the above methods can be combined.

[0121] After cell lysis is completed, the elastic membrane at the sample temporary storage area 620 deforms and expands downward, and the sample lysate in the sample reservoir 100 enters the sample temporary storage area 620 through the first sample temporary storage channel section 440 and the second sample temporary storage channel section 530 in sequence.

[0122] The elastic membrane in the second section 6122 of the second transfer section is then deformed and expanded downward, while the elastic membrane in the sample temporary storage area 620 is slowly reset upward. During this process, the sample and lysate mixture flows sequentially through the second branch channel, second section, third section 523, the second branch channel, first section, third section 433, the main channel 410, the first branch channel, first section, second section 422, and the first branch channel, second section, second section 512, before arriving in the second section 6122 of the second transfer section. As the sample and lysate mixture flows through the main channel 410, the nucleic acids in the sample are attracted to the magnetic particles. The elastic membrane in the sample temporary storage area 620 is then deformed and expanded downward, while the elastic membrane in the second section 6122 of the second transfer section is slowly reset upward. The substances in the second section 6122 of the second transfer section are transferred in the opposite direction of the aforementioned path, returning to the sample temporary storage area 620. Repeat the above steps, oscillating the sample lysate multiple times between the second transfer section's second zone 6122 and the sample temporary storage area 620 to ensure that as many nucleic acids as possible are adsorbed onto the magnetic particles. Finally, the magnetic element is energized, securing the magnetic particles. The waste sample lysate remains within the sample temporary storage area 620, and the elastic membrane in the sample temporary storage area 620 is deformed and expanded downward. The elastic membrane in the sample temporary storage area 620 is then slowly reset upward, forcing the waste liquid into the sample reservoir 100.

[0123] The elastic membrane at the first reagent storage area 631 deforms and expands downward, and the cleaning liquid in the first reagent tank 210 flows through the first section and first area 451 of the reagent temporary storage flow channel and the second section and first area 541 of the reagent temporary storage flow channel into the first reagent storage area 631 .

[0124] The elastic membrane in the third section 6123 of the second transfer section is deformed and expanded downward, while the elastic membrane in the first section 631 of the reagent storage area is slowly reset upward. During this process, the cleaning fluid flows sequentially through the second branch channel, second section 1 section 521, the second branch channel, first section 1 section 431, the main channel 410, the first branch channel, first section 4 section 424, and the first branch channel, second section 4 section 514, before arriving in the third section 6123 of the second transfer section. As the cleaning fluid flows through the main channel 410, it cleans the nucleic acids adsorbed on the magnetic particles and removes other impurities. Then, the elastic membrane in the first section 631 of the reagent storage area is deformed and expanded downward, while the elastic membrane in the third section 6123 of the second transfer section is slowly reset upward. The substances in the third section 6123 of the second transfer section are transferred in the opposite direction of the aforementioned path, returning to the first section 631 of the reagent storage area. Repeating the above steps, allowing the cleaning liquid to oscillate multiple times between the third zone 6123 of the second transfer section and the first reagent storage zone 631, can more thoroughly remove impurities and optimize the cleaning effect. Ultimately, the magnetic element secures the magnetic particles, and the waste cleaning liquid remains in the first reagent storage zone 631. The elastic membrane in the first reagent storage zone 631 is deformed and expanded downward. Then, the elastic membrane in the first reagent storage zone 631 is slowly reset upward, forcing the waste liquid into the first reagent tank 210.

[0125] The elastic membrane at the second reagent storage area 632 deforms and expands downward, and the cleaning liquid in the second reagent tank 220 flows through the first section second area 452 of the reagent temporary storage flow channel and the second section second area 542 of the reagent temporary storage flow channel into the second reagent storage area 632 in sequence.

[0126] The elastic membrane in the fourth section 6124 of the second transfer section is deformed and expanded downward, while the elastic membrane in the second section 632 of the reagent temporary storage area is slowly reset upward. During this process, the cleaning fluid flows sequentially through the second branch channel, second section 2 section 522, the second branch channel, first section 2 section 432, the main channel 410, the first branch channel, first section 5 section 425, and the first branch channel, second section 5 section 515, before arriving in the fourth section 6124 of the second transfer section. As the cleaning fluid flows through the main channel 410, it cleans the nucleic acids adsorbed on the magnetic particles and removes other impurities. Then, the elastic membrane in the second section 632 of the reagent temporary storage area is deformed and expanded downward, while the elastic membrane in the fourth section 6124 of the second transfer section is slowly reset upward. The substances in the fourth section 6124 of the second transfer section are transferred in the opposite direction of the aforementioned path, returning to the second section 632 of the reagent temporary storage area. Repeating the above steps, oscillating the cleaning fluid multiple times between the fourth zone 6124 of the second transfer section and the second reagent storage zone 632, can more thoroughly remove impurities and optimize the cleaning effect. Ultimately, the magnetic element secures the magnetic particles, and the waste cleaning fluid remains in the second reagent storage zone 632. The elastic membrane in the second reagent storage zone 632 is deformed and expanded downward. Then, the elastic membrane in the second reagent storage zone 632 is slowly reset upward, forcing the waste fluid into the second reagent tank 220.

[0127] The elastic membrane in the third reagent storage area 633 deforms and expands downward, and the eluent in the third reagent tank 230 flows through the first section three areas 453 and the second section three areas 543 of the reagent temporary storage flow channel into the third reagent storage area 633 in sequence.

[0128] The elastic membrane in the first section 6121 of the second transfer section is deformed and expanded downward, while the elastic membrane in the third section 633 of the reagent storage area is slowly reset upward. During this process, the cleaning fluid flows sequentially through the second branch channel, second section, fourth section 524, the second branch channel, first section, fourth section 434, the main channel 410, the first branch channel, first section, first section 421, and the first branch channel, second section, first section 511, before arriving in the first section 6121 of the second transfer section. As the eluent flows through the main channel 410, it separates the nucleic acids from the magnetic particles, freeing them from the eluent and allowing them to enter the first section 6121 of the second transfer section. Then, the elastic membrane in the third section 633 of the reagent storage area is deformed and expanded downward, while the elastic membrane in the first section 6121 of the second transfer section is slowly reset upward. The substances in the first section 6121 of the second transfer section are transferred in the opposite direction of the aforementioned path, returning to the third section 633 of the reagent storage area. Repeat the above steps, allowing the eluent to oscillate multiple times between the first zone 6121 of the second transfer section and the third reagent storage zone 633 to fully elute the nucleic acid. Finally, the magnetic element fixes the magnetic particles, and the elastic membrane in the third reagent storage zone 633 is in a downwardly deformed and expanded state.

[0129] Then, the elastic membrane at the first transfer section 611 deforms and expands downward, while the elastic membrane at the reagent temporary storage area 3 633 slowly returns to its original position upward. During this process, the eluent flows sequentially through the second branch channel, section 4, section 524, section 4, section 4, section 4, section 4, section 534, section 54, section 65, section 66, section 67, section 68, section 69, section 70, section 71, section 72, section 73, section 74, section 75, section 76, section 77, section 78, section 79, section 80, section 81, section 82, section 83, section 84, section 85, section 87, section 88, section 89, section 90, section 91, section 92, section 93, section 94, section 95, section 96, section

[0130] The elastic membrane at the fourth reagent storage area 634 deforms and expands downward, and the PCR reaction solution in the fourth reagent tank 240 flows sequentially through the fourth section 454 of the first reagent storage flow channel and the fourth section 544 of the second reagent storage flow channel into the fourth reagent storage area 634.

[0131] The elastic membrane in the first transfer section 611 continues to deform and expand downward, while the elastic membrane in the reagent storage area 4 634 slowly returns to its original position upward. During this process, PCR reaction solution 1 in the reagent storage area 4 634 flows sequentially through the second branch channel, section 5, section 525, the second branch channel, section 5, section 435, the main channel 410, the first branch channel, section 3, section 423, and the first branch channel, section 3, section 513, before arriving in the first transfer section 611. PCR reaction solution 1 mixes with the nucleic acid eluate in the first transfer section 611 to initiate the nested first PCR reaction.

[0132] Preferably, multiple fifth reagent cells 480 are further provided within the first plate 400. The fifth reagent cells 480 store a second PCR reaction solution, which can provide the required primers, enzymes, magnesium ions, DNTP, and other substances for the second PCR reaction. Primers can specifically amplify nucleic acids. Each fifth reagent cell 480 can contain the same primer or different primers. A single fifth reagent cell 480 can also contain multiple primers, which can be freeze-dried or in liquid form. Since multiple fifth reagent cells 480 are provided, multiple PCR reactions can be completed on the same test kit by extracting the sample only once, making it more convenient to use.

[0133] A fourth cover 340 is provided on the top of each fifth reagent cell 480 , and the fourth cover 340 seals the substance in each fifth reagent cell 480 to prevent foreign substances from entering and affecting its performance.

[0134] The third plate 600 is provided with a plurality of amplification reaction areas 640, each formed by an elastic membrane. The amplification reaction areas 640 are connected to the first transfer section 611 via a first amplification flow channel, and the fifth reagent cell 480 is connected to the amplification reaction areas 640 via a second amplification flow channel. The elastic membrane in the amplification reaction areas 640 is deformed and expanded downward, while the elastic membrane in the first transfer section 611 is slowly reset upward. During this process, the reaction solution from the first PCR reaction enters the amplification reaction areas 640. Furthermore, the second PCR reaction solution in the fifth reagent cell 480 also enters the amplification reaction areas 640. After the second PCR reaction solution mixes with the reaction solution from the first PCR reaction, a second PCR reaction is completed in the amplification reaction areas 640.

[0135] In this embodiment, pre-PCR lysis, washing, and other steps can be integrated with the PCR reaction into a single test cartridge, making it extremely quick and convenient. Before the amplification reaction zone 640 deforms and expands downward, the PCR reaction solution 2 is separated from the other reagents, preventing them from interfering with each other and ensuring greater stability during the preceding steps. Furthermore, a nested PCR step enhances detection specificity and sensitivity.

[0136] Specifically, the first amplification channel includes a first amplification channel first section 550, a first amplification channel second section 460, and a first amplification channel third section 560, which are connected in sequence. The second amplification channel includes a second amplification channel first section 570 and a second amplification channel second section 470. The first amplification channel first section 550, the first amplification channel third section 560, and the second amplification channel first section 570 are all disposed on the second plate 500 and pass through the second plate 500. The first amplification channel second section 460 and the second amplification channel second section 470 are both disposed on the bottom surface of the first plate 400.

[0137] The elastic membrane in the amplification reaction zone 640 deforms and expands downward, while the elastic membrane in the first transfer section 611 slowly returns to its original position upward. During this process, the reaction solution from the first PCR reaction flows sequentially through the first section 550 of the first amplification flow channel, the second section 460 of the first amplification flow channel, and the third section 560 of the first amplification flow channel into the amplification reaction zone 640. Furthermore, the second PCR reaction solution in the fifth reagent reservoir 480 flows sequentially through the second section 470 of the second amplification flow channel, the first section 570 of the second amplification flow channel, and into the amplification reaction zone 640.

[0138] During each of the above steps, some sample and reagent may remain in the flow channel and fail to reach the intended area, resulting in a certain amount of loss. Therefore, when initially calculating the dosage, it is important to leave a margin for each substance to ensure sufficient amount for each step.

[0139] When the above-mentioned nucleic acid detection box is conducting testing, the base at the bottom of the elastic membrane is connected to components such as an air pump to achieve air pressure changes. It is also provided with a temperature control module to adjust the reaction temperature around the elastic membrane, and also includes an optical module for fluorescence detection.

[0140] See Figures 1 to 5 In some embodiments, the test kit can also perform a rapid PCR reaction. This reaction requires the PCR reaction system to undergo multiple temperature ramping cycles to denature, anneal, and extend the DNA. In conventional test kits, the PCR reaction system is fixed in position and its temperature is continuously adjusted to complete the reaction. However, this slow ramping speed results in a longer reaction time. In this embodiment, the first module 031 and the second module 032 can each be equipped with a temperature control module, maintaining different constant temperatures. The reagents and sample can then be transferred between the first elastic membrane region 021 and the second elastic membrane region 022, thereby varying the temperature of the materials within the cavity formed by the elastic membrane regions. In other words, in this embodiment, different temperature zones are set up to allow the PCR reaction system to be rapidly transferred between these elastic membrane regions to complete the reaction. This eliminates the need to wait for the temperature to rise or fall, shortening the reaction time. Furthermore, increasing the number of modules allows for the transfer of materials between multiple temperature zones. The test kit is also connected to components such as an air pump to achieve air pressure changes, and also includes an optical module for fluorescence detection.

[0141] See Figure 13 and Figure 14 The base plate 011 of the main body 010 is provided with multiple material pools 012 along its length. The elastic membrane 020 forms multiple elastic membrane zones in corresponding areas. The base 030 is provided with multiple sets of first modules 031 and second modules 032 along its length. Each material pool 012, each elastic membrane zone, and each set of first modules 031 and second modules 032 correspond to each other, completing material transfer. Temperature control components, such as MCH and PTC, are located beneath each first module 031 and second module 032. The specific structure and material flow process are similar to those in the aforementioned embodiments of the material transfer mechanism and will not be further described.

[0142] In some embodiments, a nucleic acid detection device is provided, comprising the aforementioned detection cartridge. The detection cartridge is mounted on a nucleic acid detection instrument, which then performs air pressure control, temperature control, and fluorescence detection. Because the elastic membrane regions are located at the bottom of the detection cartridge, connection to the nucleic acid detection instrument is more convenient, and air pressure control, temperature control, and fluorescence detection are easier to operate.

[0143] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0144] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. Material transfer mechanism, characterized in that, include: an elastic membrane area, wherein the elastic membrane area is capable of expanding and deforming to form a cavity, and the cavity is used to accommodate a material; a material transfer channel, wherein the plurality of elastic membrane areas are connected to the material transfer channel, and when a pressure difference exists between two elastic membrane areas, the material can be transferred through the material transfer channel; The area where the material transfer channel contacts the elastic membrane area is provided with a protrusion, and the protrusion abuts against the elastic membrane area; The material transfer mechanism includes a main body, and a groove is provided on the bottom surface of the main body to form the material transfer channel; The material transfer mechanism also includes a driving component, which includes a base, a accommodating cavity is provided on the base, the elastic membrane area is connected to the top of the accommodating cavity, the elastic membrane area can expand and deform and fit into the cavity wall of the accommodating cavity, and along the cross-section perpendicular to the top to bottom direction of the accommodating cavity, the cross-sectional area of ​​the accommodating cavity gradually decreases from the top to the bottom of the accommodating cavity.

2. The material transfer mechanism according to claim 1, characterized in that: The elastic membrane area is connected to the bottom of the main body, and the main body is provided with the material transfer channel. The elastic membrane area can be pressed against the material transfer channel under the action of rebound force to close the material transfer channel.

3. The material transfer mechanism according to claim 1, characterized in that: The elastic membrane area and the groove have at least partial areas overlapping. When the pressure on the side of the elastic membrane area away from the material transfer channel is less than the pressure on the side close to the material transfer channel, the elastic membrane area expands and deforms, and the material transfer channel is opened; when the pressure on the side of the elastic membrane area away from the material transfer channel is not less than the pressure on the side close to the material transfer channel, the material transfer channel is closed.

4. The material transfer mechanism according to claim 3, characterized in that: The main body is further provided with a material pool for storing the material. The main body is further provided with a feed hole, which is communicated with the material pool, and the elastic membrane area is connected to the feed hole.

5. The material transfer mechanism according to claim 4, characterized in that: The material pool is arranged inside the main body, and a packaging film for sealing the material pool is provided on the top surface of the main body.

6. The material transfer mechanism according to claim 1, characterized in that: An air hole is provided on the base, and the top of the air hole is connected to the accommodating cavity.

7. The material transfer mechanism according to claim 1, characterized in that: In the base, the temperatures of regions corresponding to at least two of the elastic membrane regions are different.

8. A detection kit, characterized in that The material transfer mechanism comprises the material transfer mechanism according to any one of claims 1 to 7.

9. Nucleic acid detection equipment, characterized in that, Comprising the detection kit according to claim 8.

10. A material transfer method, characterized in that: include: A material transfer mechanism is provided; the material transfer mechanism includes an elastic membrane area and a material transfer channel, the elastic membrane area can expand and deform to form a cavity, the cavity is used to accommodate the material, and multiple elastic membrane areas are connected to the material transfer channel; When there is a pressure difference between any two of the elastic membrane areas, the material is transferred through the material transfer channel; The material transfer channel is provided with a protrusion in the area where it contacts the elastic membrane area. When the protrusion abuts against the elastic membrane area, the material transfer channel is closed. The material transfer mechanism includes a main body, and a groove is provided on the bottom surface of the main body to form the material transfer channel; The material transfer mechanism further includes a drive assembly, the drive assembly includes a base, a receiving cavity is provided on the base, the elastic membrane area is connected to the top of the receiving cavity, the elastic membrane area can expand and deform and fit the cavity wall of the receiving cavity, along a cross section perpendicular to the top to the bottom of the receiving cavity, The cross-sectional area of ​​the accommodating cavity gradually decreases from the top to the bottom of the accommodating cavity.

Images