Preparation method and application of metformin carbon dots

A metformin carbon dot and metformin technology, which is applied to medical preparations containing active ingredients, pharmaceutical formulations, carbon active ingredients, etc., can solve the problems of low functional neuron efficiency, limiting the wide application of neural stem cells, and decreasing neural stem cell survival rate. , to achieve the effect of promoting efficiency and maturity

CN114831975APending Publication Date: 2022-08-02JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2022-08-02

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Abstract

The invention discloses a preparation method and application of metformin carbon dots, and relates to the technical field of medicine preparation, the method comprises the following steps: S1, respectively dissolving metformin hydrochloride and citric acid in deionized water; s2, transferring the two mixed solutions into a high-pressure reaction kettle system; after the reaction is finished, taking out a reaction system, cooling to room temperature, and collecting to obtain a reaction solution; s3, filtering the reaction solution by adopting a filter membrane, and collecting to obtain filtrate; and S4, removing unreacted small molecular compounds from the filtrate in the step S3 by using a dialysis bag with the molecular weight cut-off of 500Da, and finally freeze-drying to obtain a metformin carbon dot freeze-dried sample. In the preparation process of the metformin carbon dots, the basic pharmacodynamic structure of functional small molecules is not damaged, and the pharmacological activity of the functional small molecules can still be reserved, so that the metformin carbon dots can promote neural stem cells to differentiate into neuronal cells, and compared with metformin, citric acid and a mixture of the metformin and the citric acid which have the same concentration in theory, the metformin carbon dots have a better neuronal differentiation promoting proportion.
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Description

technical field

[0001] The invention relates to the technical field of drug preparation, in particular to a preparation method and application of metformin carbon dots. Background technique

[0002] Neural stem cells can be functionally integrated into neural circuits during the process of differentiation into neurons, thereby achieving functional nerve regeneration. This feature of neural stem cells is expected to become the first choice in the treatment of most neuropathy. However, the pathological microenvironment leads to a decrease in the survival rate of neural stem cells, and the low efficiency of neural stem cells differentiation into functional neurons after transplantation in vivo limits the wide application of neural stem cells in transplantation therapy. As a first-line drug for the treatment of diabetes, metformin has been proven to promote the proliferation of neural stem cells in vivo, promote neuronal differentiation and axonal growth to establish functional...

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Embodiment Construction

[0026] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. The embodiments listed in the present invention are only used to illustrate the present invention, but not to limit the scope of the present invention. Any obvious modifications or changes made to the present invention do not depart from the spirit and scope of the present invention.

[0027] In one embodiment, a method for preparing metformin carbon dots is provided. The method includes the following steps: dissolving 33.124 mg of metformin hydrochloride (molecular weight=165.62 g / mol) and 0.1M citric acid (molecular weight=192.12 g / mol) in 10mL deionized water, ultrasonic for 10min;

[0028] Then CMCDs were synthesized by hydrothermal synthesis: the mixed solution of the two was transferred to a 50 mL autoclave system, and heated at 180 °C for ...