A method for anatomical positioning and anatomical collection of the vascular network at the base of the pig skull

Abstract

The present invention relates to the field of medical technology, and in particular to a method for anatomical positioning and anatomical collection of a pig skull base vascular network. The method for anatomical positioning and anatomical collection of a pig skull base vascular network provided by the present invention effectively simplifies the difficulty of collecting the pig skull base microvascular network pathological collection process from locating the left and right common carotid arteries in the chest cavity, locating the ascending pharyngeal artery upward, until breaking the foramen of the sella turcica upward, locating the vascular network and removing it. Instead, the pituitary fossa is directly located, and the sphenoid bone above the microvessel is removed, so as to avoid the rupture of the blood vessels during the ascending process of the common carotid artery, which affects the positioning, thereby saving the collection time and greatly improving the success rate of the pig skull base microvascular network pathological collection.

Description

Technical Field

[0001] The invention relates to the field of medical technology, and in particular to a method for anatomical positioning and anatomical collection of a pig skull base vascular network. Background Art

[0002] In non-clinical safety evaluation tests of medical devices, domestic pigs are often used for embolization tests simulating human arteriovenous malformations because their skull base microvascular network is very similar to human arteriovenous malformations. However, when the pigs need to be autopsied at the end of the test, the process of removing the embolized area is often very cumbersome and complicated. The existing technology often goes through the left and right common carotid arteries and ascending pharyngeal arteries, enters the foramina on both sides of the sella turcica, and then converges into a microvascular network at the skull base. This vascular network is the target sampling site.

[0003] The existing microvascular network collection process is: starting from the left and right common carotid arteries, explore upward to find the ascending pharyngeal artery in turn. This process requires opening the chest cavity, locating the left and right common carotid arteries, and then continuing to locate the ascending pharyngeal artery upward. Then continue upward along the ascending pharyngeal artery until the blood vessel enters the foramen of the sella turcica. At this time, the foramen needs to be broken open and continue to locate upward until it is exposed as a vascular network. This process is too cumbersome, and many middle arteries will be emitted during the ascent of the common carotid artery. These arteries are close in position and difficult to distinguish. After entering the foramen, the foramen needs to be broken open. Once the blood vessels in the foramen rupture, it will be difficult to continue to locate because the area is adjacent to the microvascular network and the blood contaminates the area. It takes an average of 1 hour to obtain the microvascular network of an animal, and any operational errors may lead to sample collection failure.

[0004] Therefore, it is necessary to provide a method for anatomical positioning and anatomical collection of the pig skull base vascular network, which can reduce the difficulty of operation and improve the success rate of collection. Summary of the invention

[0005] In view of the shortcomings of the above-mentioned background technology, the present invention provides a method for anatomical positioning and anatomical collection of the pig skull base vascular network, which solves the problems of great difficulty, long time consumption and low success rate in the process of pathological collection of the pig skull base microvascular network.

[0006] The present invention provides a method for anatomical positioning and anatomical collection of a pig skull base vascular network, which mainly comprises the following steps:

[0007] S1. After killing the experimental pigs, open the skull and remove the brain tissue and pituitary gland;

[0008] S2. Remove the temporal and occipital bones on both sides;

[0009] S3. Cut a piece of sphenoid bone with the pituitary fossa as the center and the opening of the foramen rupture as the starting point;

[0010] S4. Remove the cut sphenoid bone and carefully peel off the connective tissue, adipose tissue, etc. near the pharyngeal branch of the ascending pharyngeal artery from the vascular network to both sides to separate the N-shaped microvascular network.

[0011] Preferably, S3 is specifically as follows: taking the pituitary fossa as the center, using a precision cutter to cut a piece of sphenoid bone in a spade shape around it, ending at the opening of the foramen rupture, to avoid damaging the ascending pharyngeal artery at the foramen rupture.

[0012] Preferably, when cutting the sphenoid bone in S3, a cutter is used to cut the anterior segment of the pituitary fossa, and a bone drill is used to drill the sides and rear of the pituitary fossa.

[0013] Preferably, the cutting depth of the sphenoid bone is controlled in S3 to avoid damaging the microvascular network below the sphenoid bone.

[0014] Beneficial effects: Compared with the prior art, the method for anatomical positioning and anatomical collection of the pig skull base vascular network provided by the present invention effectively simplifies the difficulty of collecting the pig skull base microvascular network pathological collection process from locating the left and right common carotid arteries in the chest cavity - locating the ascending pharyngeal artery upwards - until breaking the foramen of the sella turcica upwards - locating the vascular network and removing it. Instead, the method directly locates the pituitary fossa and removes the sphenoid bone above the microvessels, avoiding the rupture of blood vessels during the ascending process of the common carotid artery that affects the positioning, thereby saving collection time and greatly improving the success rate of pathological collection of the pig skull base microvascular network. DETAILED DESCRIPTION

[0015] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is described in detail below in conjunction with the attached table and specific implementation methods.

[0016] Six healthy domestic pigs weighing 25-30 kg were randomly selected and numbered 1-6. No food was allowed 12 hours before the operation. The animals were sedated and anesthetized and maintained before the operation. The right femoral artery was punctured, and a catheter sheath was placed. A 4F vertebral artery / pigtail guide and a 0.035 loach guidewire were used for selective intubation. 60% diatrizoate was injected to perform angiography of the aortic arch (30 mL, 20 mL / s), brachiocephalic trunk (12 L / s, 8 mL / s), subclavian artery (8 mL, 6 / s), common carotid artery (6 mL, 4 mL / s), and vertebral artery (3 mL, 1 mL). A 5F balloon catheter or Tracker18 microcatheter was used to perform angiography of the ascending pharyngeal artery (3 mL, 1 mL / s) and external carotid artery (5 mL, 3 L / s). After the angiography was completed, the animals were killed by intravenous injection of a lethal dose of sodium pentobarbital.

[0017] Combined with the angiographic findings, conventional methods were used to collect microvascular networks for animals No. 1, 3, and 5: the skin and subcutaneous tissue were cut layer by layer, and the head and neck blood vessels were dissected starting from the aortic arch and the anterior vena cava. To prevent blood from contaminating the collection area, small blood vessels were ligated and cut during the dissection process; the skull base microvascular network was completely removed, fixed with 10% neutral buffered formalin, and kept for histological examination. The average time for collecting each microvessel was >1h.

[0018] This method was used to collect microvascular networks for animals No. 2, 4, and 6: remove the skin and muscles of the head to expose the skull, cut the skull with an electric craniotomy saw, remove the brain and pituitary gland, and remove the two vertical temporal bones and occipital bones. Locate the pituitary fossa, use a precision cutter to cut a piece of sphenoid bone in a spade shape around the pituitary fossa, and end at the opening of the rupture foramen. Be careful not to injure the ascending pharyngeal artery at the rupture foramen; gently lift the sawed sphenoid bone (be careful not to injure the blood vessels under the bone), and a dark red n-shaped vascular network can be observed, which is formed by the convergence of the pharyngeal branches of the ascending pharyngeal artery from the rupture foramen on both sides; carefully peel off the connective tissue, adipose tissue, etc. near the pharyngeal branches of the ascending pharyngeal artery from the vascular network to both sides, and separate the n-shaped microvascular network, fix it with 10% neutral buffered formalin, and keep it for histological examination. The average time for collecting each microvascular network is about 20 minutes.

[0019] Finally, it should be noted that the above description is only a preferred embodiment of the present invention. Under the guidance of the present invention, those skilled in the art can make a variety of similar expressions without violating the purpose and claims of the present invention, and such changes all fall within the scope of protection of the present invention.

Claims

1. A method for anatomical localization and anatomical collection of the vascular network of the pig skull base, characterized in that The following steps are involved: S1. Obtain the experimental pigs after death, open the skull, and remove the brain tissue and pituitary gland; S2. Remove the temporal and occipital bones on both sides; S3. Cut a piece of sphenoid bone with the pituitary fossa as the center and the opening of the foramen rupture as the starting point; S4. Remove the cut sphenoid bone and carefully peel off the connective tissue and adipose tissue near the pharyngeal branch of the ascending pharyngeal artery from the vascular network to both sides to separate the N-shaped microvascular network.

2. The method for anatomical positioning and anatomical collection of the vascular network of the pig skull base according to claim 1, characterized in that: The S3 is specifically as follows: taking the pituitary fossa as the center, a piece of sphenoid bone is cut in a spade shape around it using a precision cutter, and the cut ends at the opening of the foramen rupture to avoid damaging the ascending pharyngeal artery at the foramen rupture.

3. A method for anatomical positioning and anatomical collection of the pig skull base vascular network according to any one of claims 1 or 2, characterized in that: When cutting the sphenoid bone in S3, the anterior segment of the pituitary fossa was cut with a cutter, and the sides and posterior of the pituitary fossa were drilled with a bone drill.

4. The method for anatomical positioning and anatomical collection of the pig skull base vascular network according to claim 1, characterized in that: In S3, the cutting depth of the sphenoid bone is controlled to avoid damaging the microvascular network under the sphenoid bone.