A system and method for non-destructive acquisition of plant phenotypes

Abstract

The present application belongs to the technical field of plant phenotype collection, and particularly relates to a plant phenotype nondestructive collection system and method. The plant phenotype nondestructive collection system comprises a lifting mechanism, a rotating mechanism, a to-be-tested plant placing seat, an internal structure phenotype imaging device and a control device, and further comprises one or both of an external trait phenotype imaging device and a physiological phenotype imaging device. The rotating mechanism is connected with the lifting mechanism, the lifting mechanism can drive the rotating mechanism to move in the vertical direction, the rotating mechanism comprises a horizontal rotating frame, and the internal structure phenotype imaging device and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device are fixed on the horizontal rotating frame to form an imaging area. Thus, by using the plant phenotype nondestructive collection system, the internal structure phenotype, the external trait phenotype and / or the physiological phenotype of the to-be-tested plant can be collected at the same time without moving the to-be-tested plant, and the accuracy of the phenotype collection of the to-be-tested plant is improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of plant phenotype collection, and particularly relates to a plant phenotype nondestructive collection system and method. BACKGROUND

[0002] Plant phenotype research is an important research direction in the field of botany, mainly through identification and analysis of external trait phenotype (such as plant leaf shape, biomass, etc.), physiological phenotype (such as plant leaf temperature, water content, pigment distribution or stress response, etc.) and internal structure phenotype (such as the internal structure of plant ear and root), and the influence of different environmental conditions on plant growth conditions, yield, quality, etc.

[0003] With the continuous development of research in genetic breeding, plant biology, etc., there is an increasingly strong demand for collecting the external trait phenotype, physiological phenotype and internal structure phenotype of the plant at the same time and at the same position, i.e. the morphological parameters and physiological and biochemical parameters of the plant, in order to comprehensively and accurately analyze the phenotype of the plant.

[0004] However, the current analysis methods for plants are as follows:

[0005] 1. The part of the plant is removed for destructive collection, and then placed in different imaging instruments in turn for observation, which cannot perform time-continuous collection and analysis on the same individual.

[0006] 2. The whole plant is placed in multiple linkage collection rooms with different functions in turn to collect different phenotypes of the plant. However, due to the shaking or deviation of flexible plants during transmission or manual handling, the plant needs to be collected after it is stationary, and the posture of the plant at the current stationary time is often different from that at the previous stationary time, and sometimes manual alignment is required, so the plant phenotype cannot be accurately collected and the collection time is long.

[0007] It can be seen that the current collection methods and collection equipment for plants cannot meet the collection of the external trait phenotype, physiological phenotype and internal structure phenotype of the plant at the same time and at the same position. SUMMARY

[0008] (I) Technical problem to be solved

[0009] In order to solve the above problems of the prior art, the present application provides a plant phenotype nondestructive collection system and method, which can simultaneously collect the internal structure phenotype, external trait phenotype and / or physiological phenotype of the plant to be tested.

[0010] (II) Technical solution

[0011] In order to achieve the above object, the main technical scheme adopted by the present application comprises:

[0012] The present application provides a plant phenotype non-destructive collection system, comprising a rotating mechanism, a lifting mechanism, a plant to be tested placing seat, an internal structure phenotype imaging device, and a control device, and further comprising one or both of an external trait phenotype imaging device and a physiological phenotype imaging device;

[0013] The rotating mechanism is connected with the lifting mechanism, and the lifting mechanism can drive the rotating mechanism to move in the vertical direction;

[0014] The rotating mechanism comprises a horizontal rotating frame which can rotate around a vertical axis, and the internal structure phenotype imaging device and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device are fixed on the horizontal rotating frame to form an imaging area;

[0015] The plant to be tested placing seat is located below the imaging area or at the lower part of the imaging area;

[0016] The control device is communicatively connected with the rotating mechanism, the lifting mechanism, the internal structure phenotype imaging device, and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device;

[0017] The control device controls the lifting mechanism and the horizontal rotating frame to drive the internal structure phenotype imaging device and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device to move in the vertical direction and rotate along the central axis of the horizontal rotating frame, so as to realize the internal structure phenotype collection of the internal structure phenotype imaging device on the plant to be tested arranged on the plant to be tested placing seat, and the external trait phenotype and / or physiological phenotype collection of the external trait phenotype imaging device and / or the physiological phenotype imaging device on the plant to be tested.

[0018] Preferably, the horizontal rotating frame is a ring-shaped member;

[0019] The rotating mechanism further comprises a lifting beam, a slewing motor, and a plurality of object carriers;

[0020] The lifting beam is connected with the lifting mechanism, the horizontal rotating frame is connected with the lifting beam and located below the lifting beam, and the plurality of object carriers are arranged in a circumferential manner on the horizontal rotating frame and detachably connected with the horizontal rotating frame;

[0021] The slewing motor is connected with the horizontal rotating frame to drive the horizontal rotating frame to rotate;

[0022] The plurality of object carriers carry the imaging devices, and the imaging collection axes of all the imaging devices are at the same height.

[0023] Preferably, the horizontal rotating frame comprises an outer ring body, an inner ring body, and a rolling body;

[0024] The inner ring body is connected with the lifting cross beam, the outer ring body is sleeved outside the inner ring body, the accommodating grooves for accommodating the rolling bodies are arranged on the outer ring body and the inner ring body, the outer ring body and the inner ring body are relatively rotatable, and the rotary motor is connected with the outer ring body;

[0025] A plurality of groups of mounting holes are arranged on the outer ring body in a circumferential direction at equal intervals, each group of mounting holes comprises two mounting holes located at the same diameter, and the object support is detachably mounted on the outer ring body through the mounting holes;

[0026] The horizontal rotating frame further comprises an upper sealing band and a lower sealing band.

[0027] The upper sealing band and the lower sealing band are respectively arranged at the upper end and the lower end of the horizontal rotating frame, and seal the gap between the outer ring body and the inner ring body.

[0028] Preferably, the lifting mechanism comprises a main linear movement assembly.

[0029] The main linear movement assembly comprises a vertical support, a lead screw, a lead connection piece, a motor, a first lifting connecting piece and an auxiliary guide rail.

[0030] The lead screw is rotatably fixed on the vertical support, the lead connection piece is sleeved on the lead screw, and the lead connection piece is connected with the lifting cross beam of the rotating mechanism.

[0031] The motor is connected with the lead screw, drives the lead screw to rotate, and drives the rotating mechanism on the lead connection piece to move along the lead screw in the vertical direction.

[0032] The auxiliary guide rail is installed on the vertical support and is provided with the auxiliary guide rail on both sides of the lead screw.

[0033] The lead connection piece is connected with the lifting cross beam through the first lifting connecting piece, and a guide rail groove matched with the auxiliary guide rail is further arranged on the first lifting connecting piece.

[0034] Preferably, the lifting mechanism further comprises a secondary linear movement assembly.

[0035] The secondary linear movement assembly comprises a secondary support, a linear guide rail and a second lifting connecting piece, the linear guide rail is installed on the secondary support, the second lifting connecting piece is capable of sliding on the linear guide rail, and the second lifting connecting piece is connected with the lifting cross beam.

[0036] The vertical support and the secondary support are oppositely arranged on both sides of the horizontal rotating frame.

[0037] Preferably, a two-coordinate sliding table is further arranged, and the plant placement seat is connected to the two-coordinate sliding table to realize two-coordinate movement of the plant placement seat.

[0038] The two-coordinate sliding table comprises two linear modules respectively extending along the X-axis and the Y-axis, and the X-axis linear module is slidingly connected above the Y-axis linear module.

[0039] Preferably, a protective cover is further included;

[0040] The rotating mechanism, the lifting mechanism and the plant placement seat are all arranged in the protective cover;

[0041] A window is arranged on the protective cover and corresponds to the position of the plant placement seat, and the window is a transparent window.

[0042] The present application further provides a plant phenotype nondestructive collection method using the above plant phenotype nondestructive collection system.

[0043] The steps are as follows:

[0044] S1, placing a living plant to be tested on the plant placement seat;

[0045] S2, controlling the lifting mechanism to drive the horizontal rotating frame with one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device to move to a specified height;

[0046] S3, controlling the horizontal rotating frame to rotate at least one circle around the plant to be tested in the horizontal plane, and controlling one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device to collect the internal phenotype, the trait phenotype and / or the physiological phenotype of the plant to be tested together or successively;

[0047] If the height range of the image to be collected on the plant to be tested exceeds the imaging range of one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device at one height, the height range is divided into multiple specified heights to repeat steps S2 and S3.

[0048] Preferably, in step S2, according to actual needs, one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device are fixed on the horizontal rotating frame to form an imaging area.

[0049] The external trait phenotype imaging device is a visible light imager.

[0050] The physiological phenotype imaging device includes one or more of the following imagers:

[0051] The hyperspectral imager, the fluorescence imager and the infrared light imager.

[0052] The internal structure phenotype imaging device is a CT device, which includes an X-ray source and an X-ray detector, and the X-ray source and the X-ray detector are matchedly installed on the horizontal rotating frame.

[0053] Preferably, the step S3 comprises two sub-steps, one of which is performed first and the other is performed later.

[0054] S3.1, the control device controls the horizontal rotating frame to rotate 360 degrees around the plant to be tested in a horizontal plane, and controls the internal structure phenotype imaging device to collect the internal structure phenotype of the plant to be tested;

[0055] S3.2, the control device controls the horizontal rotating frame to rotate to a plurality of preset angles in turn around the plant to be tested, and at each preset angle, the control device controls the external trait phenotype imaging device and / or the physiological phenotype imaging device to collect the external trait phenotype and / or the physiological phenotype of the plant to be tested.

[0056] (III) Beneficial effects

[0057] The beneficial effects of the present application are:

[0058] The plant phenotype non-destructive collection system and method provided by the present application, by arranging the internal structure phenotype imaging device, the external trait phenotype imaging device and / or the physiological phenotype imaging device on the horizontal rotating frame which can rotate around the vertical axis, the internal structure phenotype imaging device is used to collect the internal structure phenotype of the plant, the appearance trait phenotype imaging device is used to collect the external trait phenotype of the plant and / or the physiological phenotype imaging device is used to collect the physiological phenotype of the plant, the horizontal rotating frame rotates in the horizontal plane to drive the internal structure phenotype imaging device, the external trait phenotype imaging device and / or the physiological phenotype imaging device to collect the plant, so that the internal structure phenotype, the external trait phenotype and / or the physiological phenotype of the plant to be tested can be collected at the same time without moving (i.e. at the same position), the accuracy of the phenotype collection of the plant to be tested is improved, and the horizontal rotating frame is driven by the lifting mechanism to move vertically and linearly, which can be suitable for plants with large height, and clear collection of different height positions of the plant can be realized. BRIEF DESCRIPTION OF DRAWINGS

[0059] Figure 1 It is a front view schematic diagram of the plant phenotype non-destructive collection system provided in the specific embodiment.

[0060] Figure 2 It is a front view schematic diagram of the plant phenotype non-destructive collection system provided in the specific embodiment. Figure 1 It is a front view schematic diagram of the plant phenotype non-destructive collection system provided in the specific embodiment.

[0061] Figure 3 It is an enlarged view of part A in the specific embodiment. Figure 1

[0062]

Explanation of reference signs

[0063] ​1: Rotating mechanism; 101: Horizontal rotating frame; 1011: Outer ring body; 1012: Inner ring body; 1013: Rolling element; 1014: Upper sealing strip; 1015: Lower sealing strip; 102: Lifting beam; 103: Rotary motor; 104: Loading rack;

[0064] 201: Main linear motion assembly; 2011: Vertical support; 2012: Lead screw; 2013: Lead connector; 2014: Motor; 2015: First lifting connector; 20151: Guide rail groove; 2016: Auxiliary guide rail; 2017: Fixed base; 202: Secondary linear motion assembly; 2021: Secondary support; 2022: Linear guide rail; 2023: Second lifting connector; 203: Cable chain;

[0065] 3: Placement base for the plant to be tested;

[0066] 4: Base. Detailed Implementation

[0067] To better explain and facilitate understanding of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0068] Example 1

[0069] like Figures 1-3 As shown, this embodiment provides a non-destructive plant phenotyping system. This system includes a rotating mechanism 1, a lifting mechanism, a plant placement seat 3, an internal structure phenotyping device, and a control device. It also includes one or more of an external phenotyping device and a physiological phenotyping device. The rotating mechanism 1 is connected to the lifting mechanism, which can move the rotating mechanism 1 vertically. The rotating mechanism 1 includes a horizontal rotating frame 101, which can rotate along a vertical axis, i.e., rotate horizontally. One or more of the internal structure phenotyping device and the external phenotyping device and physiological phenotyping device are fixed to the horizontal rotating frame 101 and can rotate horizontally with the frame. One or both of the internal structure phenotypic imaging device, external trait phenotypic imaging device, and physiological phenotypic imaging device form their respective imaging regions, meaning each can acquire images of a certain range of areas it faces. The internal structure phenotypic imaging device is used to acquire the internal structure phenotype of the plant, the external trait phenotypic imaging device is used to acquire the external trait phenotype of the plant, and the physiological phenotypic imaging device is used to acquire the physiological phenotype of the plant. The plant placement seat 3 is located below the imaging region and is used to place the plant to be tested. When the plant to be tested is placed on the plant placement seat 3, it can enter the imaging region, or the plant placement seat 3 is located at the bottom of the imaging region, in which case the plant to be tested will definitely be within the imaging region when placed on the plant placement seat 3. This situation is particularly suitable for cases where the plant is relatively short.

[0070] The control device is in communication connection with the rotating mechanism 1, the lifting mechanism, the internal structure phenotype imaging device, and one or two of the external trait phenotype imaging device and the physiological phenotype imaging device. The control device controls the lifting mechanism to drive the horizontal rotating frame 101 to lift, and in turn drives the internal structure phenotype imaging device, the external trait phenotype imaging device, and the physiological phenotype imaging device to lift in the vertical direction. The control device also controls the horizontal rotating frame 101 to rotate, and in turn drives one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device, and the physiological phenotype imaging device to rotate along the central axis of the horizontal rotating frame 101, so as to realize the internal structure phenotype collection of the internal structure phenotype imaging device on the plant to be detected arranged on the plant placing seat 3, the external trait phenotype collection of the external trait phenotype imaging device on the plant to be detected, and / or the physiological phenotype collection of the physiological phenotype imaging device on the plant to be detected.

[0071] The plant phenotype nondestructive collection system of the embodiment can realize the collection of the trait phenotype and the physiological phenotype of the plant to be detected at the same time without moving (i.e., at the same position) the plant to be detected, and improve the accuracy of the collection of the plant phenotype. The "same time" is not limited to the simultaneous start of the internal structure phenotype imaging device, the external trait phenotype imaging device, and the physiological phenotype imaging device to collect images, but is relative to the prior art which needs to move the plant to collect images in sequence. The plant phenotype nondestructive collection system can complete the collection of one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device, and the physiological phenotype imaging device without moving the plant to be detected, and the collection of each imaging is performed simultaneously or in sequence, and the time is greatly shortened. Therefore, the "same time" is used to describe the collection. The shortening of the collection time can further reduce or even ignore the error of the image data due to the growth of the plant, and the research result based on the image data is more accurate. In addition, the plant phenotype nondestructive collection system of the embodiment can realize nondestructive detection, and can collect the image data of the same plant at different growth stages. The plant phenotype nondestructive collection system has important significance for the research of the plant. Moreover, the collection of the plant to be detected can be completed without moving the plant to be detected, the influence of the body change of the plant to be detected on the collected image data is reduced, and the research result based on the image data is more accurate.

[0072] Further, when the plants are small in height, the lifting mechanism can not be provided. The horizontal rotating frame 101 is vertically linearly moved by the lifting mechanism, which is suitable for plants with large height, so that the plants at different height positions can be clearly collected. Meanwhile, the lifting horizontal rotating frame 101 can be lifted to a high position during the process of placing the plants on the plant placing seat 3, so as to make room for placing the plants, and the plants can be placed more conveniently.

[0073] It should be noted that the phenotypes obtained in the internal structure phenotype imaging device, the physiological phenotype imaging device and / or the external trait phenotype imaging device in the embodiment are the structural phenotypes obtained from the internal phenotype images by using existing models, the physiological phenotypes obtained from the physiological phenotype images by using existing models, and the external trait phenotypes obtained from the external trait phenotype images by using existing models.

[0074] In the embodiment, the horizontal rotating frame 101 is a ring-shaped member, and one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device are arranged along the circumference of the ring-shaped member. The imaging collection axes of the imaging devices are at the same height, i.e., the heights of the centers of the collected images of the imaging devices are the same, which is beneficial to selecting the internal and external image data of the same position on the plants after collection.

[0075] In the embodiment, the internal structure phenotype imaging device is a CT device, which includes an X-ray source and an X-ray detector, i.e., a transmitting end and a receiving end. The X-ray source and the X-ray detector are installed along the circumference of the horizontal rotating frame 101, fixed on the horizontal rotating frame 101, and symmetrically arranged relative to the plants to be detected, so as to be matchedly installed. The control device is in communication connection with the X-ray source and the X-ray detector, and controls the start and stop of the X-ray source and the X-ray detector.

[0076] In the embodiment, the light source is also included, and the external trait phenotype imaging device is a visible light imager, and the physiological phenotype imaging device includes a hyperspectral imager, a fluorescence imager and an infrared light imager, and the hyperspectral imager, the fluorescence imager, the visible light imager and the infrared light imager are sequentially arranged in a clockwise direction in a top view. The control device is in communication connection with the light source, the hyperspectral imager, the fluorescence imager, the visible light imager and the infrared light imager, and controls the start and stop of them. The light source has two setting modes. The first mode is to set one light source, which is fixed above the imaging area and irradiates towards the imaging area. The second mode is that the hyperspectral imager, the fluorescence imager, the visible light imager and the infrared light imager each match one light source, which is fixed relative to the matched imager and provides light for the current irradiation position of each. The first mode is relatively simple and convenient to operate, but may cause backlight of the lower part of the plant, and the imaging effect is poor. Of course, a light source can be additionally arranged at the lower part of the device, which irradiates towards the imaging area and obliquely upwards to supplement light for the lower part of the plant. The second mode is helpful to clearly collect images of the current irradiation position of the imager, but the structure and operation are relatively complex. Specifically, the light source can be a wide-spectrum white light source, and further can be a wide-spectrum LED light source, which provides excitation light or serves as illumination light for the hyperspectral imager, the fluorescence imager, the visible light imager and the infrared light imager.

[0077] Of course, in other embodiments, the imaging instrument of the physiological phenotype imaging device can be one or more of the above, and the X-ray source, the X-ray detector and the one or more instruments are arranged at appropriate intervals along the circumference of the horizontal rotating frame 101, which is convenient for installation and collection without interference, and meanwhile, the collection axes of the X-ray source, the X-ray detector and the one or more instruments are all at the same height.

[0078] Specifically, in addition to the horizontal rotating frame 101, the rotating mechanism 1 also includes a lifting cross beam 102, a slewing motor 103 and a plurality of object carriers 104.

[0079] The lifting cross beam 102 is connected with a lifting mechanism, and the lifting mechanism can drive the lifting cross beam 102 to move up and down. When one total light source is arranged, the light source is fixed on the lifting cross beam 102.

[0080] The horizontal rotating frame 101 is connected to the lifting cross beam 102 and is arranged below the lifting cross beam 102, and the lifting mechanism drives the lifting cross beam 102 to move linearly up and down, thereby driving the horizontal rotating frame 101 to move linearly up and down.

[0081] A plurality of carriers 104 are arranged circumferentially on the horizontal rotating frame 101 and detachably connected with the horizontal rotating frame 101, the plurality of carriers 104 rotate with the rotation of the horizontal rotating frame 101, the plurality of carriers 104 respectively carry hyperspectral imagers, fluorescence imagers, visible light imagers, infrared light imagers, X-ray sources and X-ray detectors, the imagers, the X-ray source and the X-ray detector are detachably fixed on the carriers 104. In order to ensure the angle of imaging, the fixing structure between the carriers 104 and the horizontal rotating frame 101 and the fixing structure between the imagers, the X-ray source and the X-ray detector and the carriers 104 all have directional effects. For example, a plurality of mounting holes are arranged equidistantly at the bottom end of the horizontal rotating frame 101, and a slot is arranged, a plurality of carriers 104 are inserted into the slot and fixed with the horizontal rotating frame 101 by bolts according to actual needs, and the carriers 104 are in a suspended state. There are fixing holes or fixing slots in the vertical direction on the carriers 104, and the imagers, the X-ray source and the X-ray detector can only be fixed with the carriers 104 along this direction. When a plurality of light sources are arranged corresponding to the imagers, the imager and the matching light source are fixed on the same carrier 104 to ensure that the positions of the two are relatively fixed. It should be noted that, in order to clearly show the mechanism, Figure 1 In fact, other carriers 104 are also arranged along the circumference of the horizontal rotating frame 101.

[0082] The rotary motor 103 is connected with the horizontal rotating frame 101 and is used to drive the horizontal rotating frame 101 to rotate. The control device is in communication connection with the rotary motor 103 and controls the opening and closing of the rotary motor 103.

[0083] Further, as Figure 2As shown, the horizontal rotating frame 101 comprises an outer ring body 1011, an inner ring body 1012 and rolling bodies 1013. The outer ring body 1011 is sleeved on the outside of the inner ring body 1012, and the outer ring body 1011 and the inner ring body 1012 are provided with accommodating grooves accommodating the rolling bodies 1013. The outer ring body 1011 and the inner ring body 1012 can rotate relative to each other through the rolling bodies 1013, and the outer ring body 1011 can rotate by 360 degrees. The inner ring body 1012 is connected with the lifting cross beam 102 and does not rotate. The rotary motor 103 is connected with the outer ring body 1011 to drive the outer ring body 1011 to rotate. The mounting holes mentioned above are circumferentially and equidistantly arranged on the bottom end of the outer ring body 1011. The object carrier 104 is detachably mounted on the outer ring body 1011 through the mounting holes and extends to the lower side of the horizontal rotating frame 101. Thus, one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device are connected to the outer ring body 1011 through the object carrier 104. The rotation of the outer ring body 1011 drives the object carrier 104 to rotate, and further drives one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device to rotate. It should be noted that when only the internal structure phenotype imaging device is arranged, the object carrier 101 only carries the internal structure phenotype imaging device; when the internal structure phenotype imaging device and the external trait phenotype imaging device are arranged, the object carrier 101 simultaneously carries the internal structure phenotype imaging device and the external trait phenotype imaging device and the two devices have the same collection height; when the internal structure phenotype imaging device and the physiological phenotype imaging device are arranged, the object carrier 101 simultaneously carries the internal structure phenotype imaging device and the physiological phenotype device and the two devices have the same collection height; when the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device are arranged, the object carrier 101 simultaneously carries the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype device and the three devices have the same collection height.

[0084] The horizontal rotating frame 101 further comprises an upper sealing band 1014 and a lower sealing band 1015, which are respectively arranged at the upper end and the lower end of the horizontal rotating frame 101 to seal the gap between the outer ring body 1011 and the inner ring body 1012, so as to prevent the lubricating oil in the rolling body 1013 accommodating groove from leaking and ensure the lubrication of the rolling body 1013.

[0085] As shown in FIG. 1, the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device are arranged on the horizontal rotating frame 101. The internal structure phenotype imaging device is arranged on the object carrier 104, and the external trait phenotype imaging device and the physiological phenotype imaging device are arranged on the lifting cross beam 102. Figure 3As shown, the lifting mechanism includes a main linear movement assembly 201, which includes a vertical support 2011, a lead screw 2012, a lead connection piece 2013 and a motor 2014. The lead screw 2012 is vertically oriented and rotatably fixed on the vertical support 2011 through a fixing seat 2017, the lead connection piece 2013 is sleeved on the lead screw 2012 and connected with the lifting cross beam 102, and the motor 2014 is connected with the lead screw 2012 to drive the rotation of the lead screw 2012 so as to drive the rotating mechanism 1 on the lead connection piece 2013 to move along the lead screw 2012 in the vertical direction. The motor 2014 is connected with a control device, and the control device controls the opening and closing of the motor 2014.

[0086] The main linear movement assembly 201 further includes a first lifting connection piece 2015 and an auxiliary guide rail 2016, the auxiliary guide rail 2016 is installed on the vertical support 2011 and is arranged on both sides of the lead screw 2012, the lead connection piece 2013 is connected with the lifting cross beam 102 through the first lifting connection piece 2015, and a guide rail groove 20151 matched with the auxiliary guide rail 2016 is further arranged on the first lifting connection piece 2015. The guide rail groove 20151 on the first lifting piece moves along the auxiliary guide rail 2016, further ensuring the stability of the movement of the rotating mechanism 1 in the vertical direction. Of course, in actual application, a single-sided auxiliary guide rail 2016 can also be arranged on the left side or the right side of the vertical support 2011, as long as it can ensure the stable operation of the rotating mechanism 1.

[0087] Specifically, the lifting mechanism further includes a secondary linear movement assembly 202, which includes a secondary support 2021, a linear guide rail 2022 and a second lifting connection piece 2023, the linear guide rail 2022 is installed on the secondary support 2021, the second lifting connection piece 2023 can slide on the linear guide rail 2022, the linear guide rail 2022 is connected with the lifting cross beam 102 through the second lifting connection piece 2023, and the vertical support 2011 and the secondary support 2021 are oppositely arranged on both sides of the horizontal rotating frame 101. By arranging the secondary linear movement assembly 202, the rotating mechanism 1 is driven to move in the vertical direction by cooperating with the main linear movement assembly 201, thereby ensuring the stability of the movement of the rotating mechanism 1 in the vertical direction. The plant phenotype nondestructive collection system further includes a base 4, and the main support and the secondary support 2021 are arranged on both sides of the base 4. A drag chain 203 can be arranged on one side of the base 4 for placing electric wires.

[0088] Specifically, the plant phenotype non-destructive collection system further comprises a two-coordinate sliding table, and the bottom end of the plant placement seat 3 is connected to the two-coordinate sliding table. The two-coordinate sliding table comprises two straight line modules extending along the X-axis and Y-axis respectively, and the X-axis straight line module is slidingly connected above the Y-axis straight line module to realize two-coordinate movement of the plant placement seat 3. The control device is in communication connection with the X-axis straight line module and the Y-axis straight line module to control the start and stop of the two modules.

[0089] Of course, the plant phenotype non-destructive collection system further comprises a protective cover which can shield X-ray radiation and prevent external environment from interfering with the test plant, such as wind blowing the plant, and the protective cover can be transparent material, and the rotating mechanism 1, the lifting mechanism and the plant placement seat 3 are all placed in the protective cover. In addition, a closable window corresponding to the position of the plant placement seat 3 can be provided on the protective cover, and the window is a transparent window which is opened when the test plant is placed and closed after the test plant is placed.

[0090] In addition, the plant phenotype non-destructive collection system further comprises a central control module, which is in communication connection with the control device, the internal structure phenotype imaging device, the external trait phenotype imaging device and / or the physiological phenotype imaging device. The collection results of the internal structure phenotype imaging device, the external trait phenotype imaging device and / or the physiological phenotype imaging device are all fed back to the central control module, and the central control module gives instructions to the control module according to the set collection scheme, and the control module controls the work of each device and mechanism according to the instructions.

[0091] Embodiment Two

[0092] The plant phenotype non-destructive collection method provided in this embodiment adopts the plant phenotype non-destructive collection system in Embodiment One, and the steps of collection are as follows:

[0093] S1, placing the living test plant on the plant placement seat 3;

[0094] S2, the control device controls the lifting mechanism to drive the horizontal rotating frame 101 with one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device to move to a specified height, i.e. the height of the detection position of the test plant;

[0095] S3, the control device controls the horizontal rotating frame 101 to rotate at least one circle around the plant to be tested in the horizontal plane, and controls one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device to collect the internal phenotype, the trait phenotype and / or the physiological phenotype of the plant to be tested together or in sequence. It should be noted that when only the internal structure phenotype imaging device is provided, the control device only controls the internal structure phenotype imaging device to collect the internal structure phenotype; when the internal structure phenotype imaging device and the external trait phenotype imaging device are provided, the control device controls the internal structure phenotype imaging device to collect the internal structure phenotype and the external trait phenotype imaging device to collect the external trait phenotype together or in sequence; when the internal structure phenotype imaging device and the physiological phenotype imaging device are provided, the control device controls the internal structure phenotype imaging device to collect the internal structure phenotype and the physiological phenotype imaging device to collect the physiological phenotype together or in sequence; when the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device are provided, the control device controls the internal structure phenotype imaging device to collect the internal structure phenotype, the external trait phenotype imaging device to collect the external trait phenotype and the physiological phenotype imaging device to collect the physiological phenotype together or in sequence.

[0096] If the height range of the image to be collected on the plant to be tested exceeds the imaging range of one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device at one height, the height range is divided into multiple specified heights, and the steps S2 and S3 are repeated.

[0097] It should be noted that the sequence of the collection of one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device is not limited, and can be performed in sequence or simultaneously.

[0098] Specifically, in step S2, according to the actual needs, one or two of the internal structure phenotype imaging device, the external trait phenotype imaging device and the physiological phenotype imaging device are fixed on the horizontal rotating frame 101 to form an imaging area. In the embodiment, the external trait phenotype imaging device is a visible light imager, and the physiological phenotype imaging device includes a hyperspectral imager, a fluorescence imager and an infrared light imager installed in sequence. The internal structure phenotype imaging device is a CT device including an X-ray source and an X-ray detector, and the X-ray source and the X-ray detector are matchedly installed on the horizontal rotating frame 101.

[0099] The light source is also included on the horizontal rotating frame 101, and there are two setting modes. The first mode is to set one light source which is fixed above the imaging area and irradiates toward the imaging area. The second mode is that the hyperspectral imager, the fluorescence imager, the visible light imager and the infrared light imager are respectively matched with one light source which is fixed relative to the matched imager and provides light for the current irradiation position. The first mode is relatively simple and convenient to operate, but may cause backlight of the lower part of the plant and poor imaging effect. Of course, a light source can be additionally arranged at the lower part of the device to irradiate toward the imaging area and obliquely upward to supplement light for the lower part of the plant. The second mode is helpful to clearly collect images of the current irradiation position of the imager, but the structure and operation are relatively complex. Specifically, the light source can be a wide-spectrum white light source, and further can be a wide-spectrum LED light source which provides excitation light or serves as illumination light for the hyperspectral imager, the fluorescence imager, the visible light imager and the infrared light imager.

[0100] If the light source is arranged at the top, the control device controls the light source to be turned on before the image is collected in step S3. If the light source is arranged corresponding to each imager, the control device controls the corresponding light source to be turned on before the imager works.

[0101] Step S3 includes the following two sub-steps, and one of the two sub-steps is executed first and the other is executed later.

[0102] S3.1, the control device controls the horizontal rotating frame 101 to rotate 360 degrees around the plant to be tested in the horizontal plane, and the control device controls the internal structure phenotype imaging device to collect the internal structure phenotype of the plant to be tested, and the collected image data is returned to the central control module;

[0103] S3.2, the control device controls the horizontal rotating frame 101 to rotate to a plurality of preset angles in turn around the plant to be tested, and at each preset angle, the control device controls the internal structure phenotype imaging device, the external trait phenotype imaging device and / or the physiological phenotype imaging device to collect the external trait phenotype and / or the physiological phenotype of the plant to be tested, and the collected image data is returned to the central control module. Of course, one or more of the visible light imager, the hyperspectral imager, the fluorescence imager and the infrared light imager can be controlled in sequence to complete the shooting of the plurality of preset angles.

[0104] For example, when the fluorescence imaging of the living plant to be tested at a single position is performed, the control device controls the lifting mechanism to drive the horizontal rotating frame to move along the z direction to a specified height and stop, and at this time, the control device controls the light source and the fluorescence imaging device to be turned on to perform optical imaging on the living plant to be tested.

[0105] Of course, in the present embodiment, image reconstruction steps can also be included, such as CT image reconstruction and optical image reconstruction. CT image reconstruction is used to reconstruct X-ray raw images into tomographic images, and optical image reconstruction is used to display and reconstruct various optical images in three dimensions. The control system can also obtain the position, angle, and other parameters corresponding to each image, and reconstruct the CT image and the optical image into an overall image.

[0106] Of course, the present plant phenotype nondestructive acquisition system has the ability to simultaneously acquire internal structure phenotypes, external trait phenotypes, and physiological phenotypes, but can also acquire only one phenotype by not installing or starting one type of phenotype acquisition device.

[0107] In the present application, unless otherwise explicitly specified and limited, a first feature is "on" or "under" a second feature, which can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature is "above", "over", and "on" the second feature, which can be that the first feature is directly above or obliquely above the second feature, or only means that the first feature is horizontally higher than the second feature. The first feature is "below", "under", and "under" the second feature, which can be that the first feature is directly below or obliquely below the second feature, or only means that the first feature is horizontally lower than the second feature.

[0108] Although the embodiments of the present application have been shown and described above, it should be understood that the above-described embodiments are exemplary and should not be construed as limiting the present application, and those of ordinary skill in the art can make changes, modifications, replacements, and variations to the above-described embodiments within the scope of the present application.

Claims

1. A system for non-destructive phenotyping of plants, characterized in that, The device comprises a rotating mechanism, a lifting mechanism, a plant placement seat, an internal structure phenotype imaging device, a light source, a protective cover, a control device, and one or both of an external trait phenotype imaging device and a physiological phenotype imaging device, and a two-coordinate sliding table. The rotating mechanism is connected to the lifting mechanism, and the lifting mechanism can drive the rotating mechanism to move in the vertical direction. The rotating mechanism comprises a horizontal rotating frame that can rotate around a vertical axis, and the internal structure phenotype imaging device and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device are fixed on the horizontal rotating frame to form an imaging area. The rotating mechanism further comprises a lifting crossbeam, a rotary motor, and a plurality of object carriers. The lifting crossbeam is connected to the lifting mechanism, the horizontal rotating frame is connected to the lifting crossbeam and located below the lifting crossbeam, and the plurality of object carriers are arranged circumferentially on the horizontal rotating frame and detachably connected to the horizontal rotating frame. The rotary motor is connected to the horizontal rotating frame to drive the horizontal rotating frame to rotate, the plurality of object carriers carry the imaging devices, and the imaging collection axes of all the imaging devices are at the same height. The light source is fixed on the lifting crossbeam. The horizontal rotating frame is a ring-shaped member comprising an outer ring body, an inner ring body, and a rolling body. The inner ring body is connected to the lifting crossbeam, the outer ring body is sleeved outside the inner ring body, a containing groove for accommodating the rolling body is arranged on the outer ring body and the inner ring body, the outer ring body and the inner ring body are connected by relative rotation, and the rotary motor is connected to the outer ring body. A plurality of groups of mounting holes are circumferentially and equidistantly arranged on the outer ring body, each group of mounting holes comprises two mounting holes located on the same diameter, and the object carriers are detachably mounted on the outer ring body through the mounting holes. The horizontal rotating frame further comprises an upper sealing strip and a lower sealing strip. The upper sealing strip and the lower sealing strip are respectively arranged at the upper end and the lower end of the horizontal rotating frame to seal the gap between the outer ring body and the inner ring body. The rotating mechanism, the lifting mechanism, and the plant placement seat are all arranged in the protective cover, and a window that can be opened and closed is arranged on the protective cover corresponding to the position of the plant placement seat. The window is a transparent window. The plant placement seat is located below or at the lower part of the imaging area, and the plant placement seat is connected to the two-coordinate sliding table to realize the two-coordinate movement of the plant placement seat. The two-coordinate sliding table comprises two straight line modules extending along the X-axis and the Y-axis respectively, and the X-axis straight line module is slidably connected to the upper part of the Y-axis straight line module. The control device is communicatively connected to the rotating mechanism, the lifting mechanism, the internal structure phenotype imaging device, and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device. The control device controls the lifting mechanism and the horizontal rotating frame to drive the internal structure phenotype imaging device and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device to move in the vertical direction and rotate along the central axis of the horizontal rotating frame, so as to realize the internal structure phenotype collection of the internal structure phenotype imaging device on the plant to be detected arranged on the plant placing seat, and realize the external trait phenotype and / or physiological phenotype collection of the external trait phenotype imaging device and / or the physiological phenotype imaging device on the plant to be detected.

2. The plant phenotype non-destructive collection system according to claim 1, wherein the lifting mechanism comprises a main linear moving assembly.

2. The plant phenotype non-destructive collection system according to claim 1, wherein the lifting mechanism comprises a main linear moving assembly. The screw rod is rotatably fixed on the vertical support, the lead connection member is sleeved on the screw rod, and the lead connection member is connected with the lifting cross beam of the rotating mechanism. The motor is connected with the screw rod and drives the rotation of the screw rod, so as to drive the rotating mechanism on the lead connection member to move in the vertical direction along the screw rod with the lead connection member. The lead connection member is connected with the lifting cross beam through the first lifting connecting piece, and a guide rail groove matched with the auxiliary guide rail is further arranged on the first lifting connecting piece.

3. The plant phenotype non-destructive collection system according to claim 2, wherein the lifting mechanism further comprises a secondary linear moving assembly.

3. The plant phenotype non-destructive collection system according to claim 2, wherein the lifting mechanism further comprises a secondary linear moving assembly. The vertical support and the secondary support are oppositely arranged on both sides of the horizontal rotating frame. Any one of the plant phenotype non-destructive collection systems according to claims 1-3 is adopted. The steps are as follows: S1, placing the living plant to be detected on the plant placing seat; 4. A method for non-destructive acquisition of plant phenotypes, characterized in that S2, the control device controls the lifting mechanism to drive the horizontal rotating frame with the internal structure phenotype imaging device and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device to move to a specified height; S3, the control device controls the horizontal rotating frame to rotate at least one circle around the plant to be detected in the horizontal plane, and controls the internal structure phenotype imaging device and one or both of the external trait phenotype imaging device and the physiological phenotype imaging device to collect the internal phenotype, the trait phenotype and / or the physiological phenotype of the plant to be detected together or successively. ​ ​ ​ If the height range of the image to be collected on the plant to be tested exceeds the imaging range of one or both of the internal structure phenotype imaging device and the external trait phenotype imaging device and the physiological phenotype imaging device at one height, the height range is divided into multiple specified heights to repeat steps S2 and S3.

5. The plant phenotype non-destructive collection method according to claim 4, characterized in that, In step S2, according to actual needs, one or both of the internal structure phenotype imaging device and the external trait phenotype imaging device and the physiological phenotype imaging device are fixed on the horizontal rotating frame to form an imaging area; The external trait phenotype imaging device is a visible light imager. The physiological phenotype imaging device includes one or more of the following imagers: Hyperspectral imager, fluorescence imager, infrared light imager; The internal structure phenotype imaging device is a CT device, which includes an X-ray source and an X-ray detector, and the X-ray source and the X-ray detector are matchedly installed on the horizontal rotating frame.

6. The plant phenotype non-destructive collection method according to claim 5, characterized in that, Step S3 includes the following two sub-steps, and one of the two sub-steps is executed first and the other is executed later: S3.1, the control device controls the horizontal rotating frame to rotate 360 degrees around the plant to be tested in the horizontal plane, while the control device controls the internal structure phenotype imaging device to collect the internal structure phenotype of the plant to be tested; S3.2, the control device controls the horizontal rotating frame to rotate to multiple preset angles in turn around the plant to be tested, while at each preset angle, the control device controls the external trait phenotype imaging device and / or the physiological phenotype imaging device to collect the external trait phenotype and / or the physiological phenotype of the plant to be tested.

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