A white light-narrow band-fluorescence integrated endoscope and its use method

Abstract

The present invention provides a white light-narrow band-fluorescence integrated endoscope and a method of using the same, comprising: a light source part, a light source control unit and an endoscope part; the light source part provides illumination light irradiated on the biological tissue to be inspected, the illumination light includes white light and narrow band light of multiple wavelengths, and the narrow band light includes fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be inspected to produce fluorescence; the light source control unit controls the type of illumination light provided by the light source part; the endoscope part comprises an endoscope detector and a notch plate; the endoscope detector is used to receive the reflected light after the illumination light is reflected by the biological tissue to be inspected and the fluorescence generated by the photosensitizer on the biological tissue to be inspected being excited by the fluorescence excitation light, and both the reflected light and the fluorescence enter the endoscope detector through the notch plate; the notch wavelength of the notch plate is equal to the wavelength of the fluorescence excitation light. Combining the multi-spectral narrowband function with the fluorescence function can significantly reduce the complexity of the endoscope structure while ensuring the imaging quality and acquisition speed.

Description

Technical Field

[0001] The present invention belongs to the field of endoscopes, and in particular relates to a white light-narrow band-fluorescence integrated endoscope and a use method thereof. Background Art

[0002] White light endoscopy, narrow-band endoscopy, polarization endoscopy and fluorescence endoscopy can provide structural signals and functional signals of tumors respectively. In particular, narrow-band signals are usually sensitive to functional information such as blood oxygen and submucosal microvessels, while polarization signals are sensitive to chiral molecules and birefringence signals. On this basis, fluorescence signals can further indicate tumors through functional information such as photosensitizer probe concentration. Multiple functional signals complement each other, and through image recognition and signal filtering technology, multi-source data can be fused and analyzed to improve the algorithm's ability to recognize tumors.

[0003] However, at present, the probes or imaging modules of chromoendoscopy, confocal micro-endoscope, ultrasonic endoscope, etc. are not compatible with white light endoscope, which actually increases the difficulty of multi-mode detection. For example, it is often difficult to make a qualitative diagnosis of gastrointestinal tumors only through white light endoscope, especially in many cases, it is necessary to further make a qualitative diagnosis of the tumor through magnifying endoscope, chromoendoscopy and pathological biopsy, and sometimes it is necessary to make auxiliary judgments on submucosal tissue. For another example, in the process of photodynamic therapy, oxygen content and photosensitizer concentration detection are two important parts of photodynamic dose monitoring. Among them, the detection of oxygen content requires the use of multi-spectral narrow-band endoscope to detect hemoglobin, while the photosensitizer concentration requires fluorescence channel detection. Under current technology, it is difficult to perform integrated detection of target tissues under the same endoscope, and it needs to be switched through mechanical devices. The working nature of the endoscope determines that the volume of the endoscope module will inevitably increase through mechanical devices, which in fact limits its scope of application. For example, the white light-microscope integrated endoscope requires the design of a complex elastic-zoom optical path, and its volume is at least doubled. Narrow-band-fluorescence endoscopes require the design of a split light path, which greatly increases the volume and reduces the utilization of other wavelengths of light. Therefore, there are no integrated endoscopes for commercial applications on the market. For example, Olympus's NBI endoscopes usually detect narrow-band reflected light at 415 and 540 nm, and the VIST uses a single wavelength combined with the RGB channels of the imaging CCD for composite staining. These methods focus on contrasting the absorption caused by microvessels and cannot detect spectral information such as lipids or autofluorescence. The Imperial College of London has developed a hyperspectral narrow-band endoscope that uses a supercontinuum light source, but the supercontinuum light source is very expensive.

[0004] The concentration monitoring of photosensitizer is the most important part of photodynamic dose monitoring. By detecting the excitation fluorescence intensity of photosensitizer, a variety of information can be obtained, such as the concentration, targeting and spatial distribution of photosensitizer. The main method for detecting the concentration of photosensitizer is fluorescence endoscope. Patent No. CN 110772208 A discloses a fluorescence endoscope device, which collects multiple fluorescence image sub-blocks of the subject through a compound eye imaging device, and then performs pixel coordinate registration on these fluorescence sub-blocks, and finally performs pixel restoration and superposition based on the pixel registration coordinates of each pixel point in each fluorescence image sub-block, thereby generating a fluorescence image of the subject. Although this method can obtain high-quality fluorescence images, it requires multiple detection channels and its volume is difficult to reduce. Therefore, its scenes in practical applications are extremely limited, and the algorithm is extremely complex, and the performance requirements of the image processing equipment are very high.

[0005] Patents CN109758094A and CN108577791A disclose a fluorescence navigation endoscope system and a method for enhancing the sensitivity of fluorescence imaging. The method separates fluorescence and white light through a split light path, which is difficult to combine with a narrow-band reflective endoscope because each additional optical channel requires more cameras and split light paths, greatly increasing the cost. On the other hand, it is difficult to integrate more optical devices into the endoscope probe.

[0006] Patent document CN109758094A discloses a microscopic endoscopy system, which uses an imaging optical fiber to extract images, thereby realizing optical path splitting outside the body. Although this is not subject to the volume limitation problem in the body, the resolution of the imaging optical fiber is limited. For example, Sumita Optics, which claims to be the industry's leading imaging optical fiber, can only achieve a resolution of 100,000 pixels, which is far from the current clinical 1080P or even 4K resolution requirements. Patent document CN108670203A discloses a narrowband-fluorescence imaging optical path, which uses 3 sensors and corresponding splitting optical paths, which greatly increases its volume and design complexity, making it difficult to apply to electronic endoscopes. Patent document CN110731748A discloses an electronic endoscope with one narrowband light and one fluorescence coupled, using two splitting optical paths and an imaging chip, and its upper limit can handle a single wavelength of reflected light and a single wavelength of fluorescence. Summary of the invention

[0007] The purpose of the present invention is to provide a white light-narrow band-fluorescence integrated endoscope and a method of using the same, which combines the white light function, the multi-spectral narrow band function and the fluorescence function, and can significantly reduce the complexity of the endoscope structure while ensuring the imaging quality and acquisition speed.

[0008] The present invention is achieved through the following technical solutions:

[0009] A white light-narrow band-fluorescence integrated endoscope, comprising: a light source part, a light source control unit and an endoscope part;

[0010] The light source part is used to provide illumination light for irradiating the biological tissue to be inspected, and the types of the illumination light include white light and narrow-band light of multiple wavelengths, and the narrow-band light includes fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be inspected to generate fluorescence;

[0011] The light source control unit is used to control the type of illumination light provided by the light source part;

[0012] The endoscope part includes an endoscope detector and a notch plate; the endoscope detector is used to receive the reflected light of the illumination light provided by the light source part after being reflected by the biological tissue to be inspected and the fluorescence generated by the photosensitizer on the biological tissue to be inspected being excited by the fluorescence excitation light provided by the light source part, so as to obtain a light signal, and both the reflected light and the fluorescence enter the endoscope detector through the notch plate; the notch wavelength of the notch plate is equal to the wavelength of the fluorescence excitation light.

[0013] Preferably, the endoscope part also includes a clamp, an endoscope detector channel is opened inside the clamp, the endoscope detector is installed in the endoscope detector channel, a groove is arranged on an end face of one end of the clamp, the groove is coaxial with and connected to the endoscope detector channel, and a notch plate is installed in the groove.

[0014] Furthermore, a light beam guiding channel is provided on the holder, and the axis of the light beam guiding channel is parallel to the axis of the endoscope detector channel; the illumination light provided by the light source part is irradiated on the biological tissue to be inspected through the light beam guiding channel.

[0015] Furthermore, two light beam guiding channels are provided, and the two light beam guiding channels are symmetrical with respect to the endoscope detector channel.

[0016] Preferably, it also includes a data acquisition part, which includes an acquisition card and a storage card. The acquisition card is used to receive the optical signal of the endoscopic detector and the imaging mode signal of the light source control unit, and process them to obtain the tissue image; the storage card is used to store the tissue image obtained by the acquisition card.

[0017] Furthermore, it also includes a display, which is used to display the tissue image obtained by the acquisition part in real time.

[0018] Preferably, the light source part includes a xenon lamp light source, a focusing lens group and a filter wheel; the focusing lens group includes a first focusing lens and a second focusing lens; the first focusing lens, the filter wheel and the second focusing lens are arranged in sequence in the light emission direction of the xenon lamp light source.

[0019] Preferably, the light source part includes a xenon lamp light source, a focusing lens group and a tunable liquid crystal filter LCTF; the focusing lens group includes a first focusing lens and a second focusing lens; the first focusing lens, the tunable liquid crystal filter LCTF and the second focusing lens are arranged in sequence in the direction of light emission of the xenon lamp light source.

[0020] The method of using the white light-narrow band-fluorescence integrated endoscope includes a white light imaging mode, a narrow band imaging mode and a fluorescence imaging mode;

[0021] If the white light imaging mode is selected, the light source control unit controls the light source part to emit white light, which is irradiated on the biological tissue to be inspected through the light guide channel, and the reflected light of the white light reflected by the biological tissue to be inspected is detected by the endoscope detector;

[0022] If the narrow-band imaging mode is selected, the light source control unit controls the light source part to emit narrow-band light with a wavelength that is not equal to the notch wavelength of the notch plate, and irradiates the narrow-band light on the biological tissue to be inspected through the light guide channel. The reflected light of the narrow-band light reflected by the biological tissue to be inspected is detected by the endoscope detector;

[0023] If the fluorescence imaging mode is selected, the light source control unit controls the light source part to emit a narrow-band light with a wavelength equal to the notch wavelength of the notch plate, which is irradiated onto the biological tissue to be inspected through the light guide channel. The photosensitizer on the biological tissue to be inspected is excited to produce fluorescence, and the narrow-band light is reflected by the biological tissue to be inspected. The reflected light and the fluorescence are irradiated onto the endoscope part together. After the reflected light is filtered out by the notch plate, the fluorescence is detected by the endoscope detector.

[0024] Furthermore, the white light-narrow band-fluorescence integrated endoscope includes a data acquisition part, which includes an acquisition card and a storage card. The acquisition card is used to receive the optical signal of the endoscope detector and the imaging mode signal of the light source control unit, and process to obtain the tissue image; the storage card is used to store the tissue image obtained by the acquisition card;

[0025] The acquisition card obtains an image tag according to the imaging mode signal fed back by the light source control unit, obtains an image according to the received light signal of the endoscopic detector, and adds an image tag to the image to obtain a tissue image.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The endoscope of the present invention can provide white light and narrow-band light of multiple wavelengths including fluorescent excitation light, that is, the complex narrow-band spectral optical path is placed at the light source end, which does not enter the body and can be expanded later as needed; a notch plate is set at the endoscope part, and when the illumination light is fluorescent excitation light, the reflected light of the fluorescent excitation light can be filtered, and only the fluorescence is allowed to enter the endoscope detector, so as to separate the reflected light of the fluorescent excitation light from the fluorescence, thereby simplifying the endoscope part entering the body through the notch optical path, and realizing the white light-narrow band-fluorescence integrated endoscope design without increasing the volume of the endoscope part through the small volume optical path, so that the endoscope part extending into the body of the present invention is small in volume and simple in structure; the reflected light of the fluorescent excitation light in the fluorescence mode is filtered out by the notch method, which solves the problem that the multi-spectrum narrow-band function and the fluorescence imaging function are difficult to integrate. The present invention realizes the integration of white light endoscope, multi-spectrum narrow-band endoscope and fluorescent endoscope, and the three share a set of light sources and a set of image acquisition equipment, realizing the integration of multiple functions and filling the gap in the field. The scheme of the present invention is reasonable, simple in structure, easy to implement, highly adaptable, and has a wide range of application scenarios. It can be used to detect oxygen content and photosensitizer concentration during photodynamic therapy and improve the integration level of endoscopes for photodynamic therapy of deep tumors.

[0028] Furthermore, the endoscope part of the present invention adopts a self-designed holder, which has an ingenious structure and low cost.

[0029] Furthermore, the data acquisition part adopts an integrated acquisition card and storage card, which can perform both data acquisition and storage and has a compact size.

[0030] The use process of the white light-narrow band-fluorescence integrated endoscope of the present invention can realize simple switching among white light imaging mode, narrow band imaging mode and fluorescence imaging mode, and realize multiple detection modes by using the same endoscope. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a block diagram of the overall structure of the white light-narrow band-fluorescence endoscope imaging system of the present invention;

[0032] Figure 2 It is a schematic diagram of the structure of the endoscope part of the present invention;

[0033] Figure 3 It is a schematic diagram of the structure of the clamp of the present invention;

[0034] Figure 4 It is a schematic structural diagram of a light source part according to a first embodiment of the present invention;

[0035] Figure 5 It is a schematic structural diagram of a light source part according to a second embodiment of the present invention;

[0036] Figure 6It is a timing gating flow chart of three modes of the present invention;

[0037] Light source part 100, endoscope part 200, data acquisition part 300, endoscope detector 210, notch plate 230, holder 240, light guide channel 241 / 242, endoscope detector channel 243, groove 244, xenon lamp light source 110, focusing lens group 120, filter wheel 130, tunable liquid crystal filter LCTF 140. DETAILED DESCRIPTION

[0038] In order to further understand the present invention, the present invention is described below in conjunction with embodiments. These descriptions are only to further explain the features and advantages of the present invention and are not intended to limit the claims of the present invention.

[0039] The present invention discloses a white light-narrow band-fluorescence endoscope imaging system, which realizes the integrated design of narrow band reflection imaging and fluorescence imaging. The idea is to simplify the optical design of the endoscope part, separate the fluorescence and narrow band light reflection bands by notching, do not increase the volume of the endoscope part that needs to enter the body, realize white light-narrow band light source excitation in the external light source, and thus realize the goal of white light-narrow band-fluorescence integrated endoscope detection.

[0040] like Figure 1 As shown, the white light-narrow band-fluorescence endoscope imaging system of the present invention is composed of three parts: a light source part 100 , a light source control unit, an endoscope part 200 , and a data acquisition part 300 .

[0041] The light source part 100 is used to provide illumination light for irradiating the biological tissue to be inspected. The types of illumination light include white light and narrow-band light of multiple wavelengths. The narrow-band light includes fluorescence excitation light. The fluorescence excitation light can excite the photosensitizer on the biological tissue to produce fluorescence. The wavelength of the fluorescence excitation light needs to be selected according to the characteristics of the photosensitizer.

[0042] The light source control unit is used to control the type of illumination light provided by the light source part 100 .

[0043] The endoscope part 200 is a detection part extending into the biological tissue to be inspected, and can capture and acquire the tissue image of the biological tissue to be inspected under the illumination of white light or narrow-band light provided by the light source part 100 to obtain a light signal.

[0044] The data acquisition part 300 receives the optical signal from the endoscope part 200 and the imaging mode signal from the light source control unit, processes and obtains the tissue image and stores it.

[0045] like Figure 2As shown, the endoscope part 200 is composed of an endoscope detector 210 and a notch plate 230. The notch wavelength of the notch plate 230 is equal to the wavelength of the fluorescent excitation light. The endoscope detector 210 and the notch plate 230 are fixed together by a clamp 240. The clamp 240 is provided with a light guide channel that can be extended into human tissue for detection. The structure of the clamp 240 is as shown in FIG. Figure 3 As shown, the structure is designed using SOLIDWORKS software and 3D printed with PLA material. This part is printed in split form and is divided into two parts, front and back, with a total of three channels. The two channels 241 and 242 with smaller diameters on both sides are light guide channels, and the channel 243 with a larger diameter in the middle is the endoscope detector channel for installing the endoscope detector 210. There is a groove 244 at the front end of the endoscope detector channel (close to the biological tissue to be inspected), which is used to fix the notch plate 230.

[0046] The endoscope detector 210 is used to receive the reflected light of the illumination light provided by the light source part 100 after being reflected by the biological tissue to be inspected and the fluorescence generated by the photosensitizer on the biological tissue to be inspected being excited by the fluorescence excitation light provided by the light source part 100. When the light source part 100 provides the fluorescence excitation light, since the notch wavelength of the notch plate 230 is equal to the wavelength of the fluorescence excitation light, the notch plate can filter out the reflected light of the biological tissue to be inspected, and can ensure that only the fluorescence reaches the endoscope detector when performing fluorescence imaging.

[0047] The design of the holder 240 can ensure that the reflected light is separated from the fluorescence, thereby ensuring that the reflected light of the tissue is filtered out when realizing fluorescence functional imaging. It is a key link in ensuring the integration of fluorescence imaging function and narrow-band imaging function.

[0048] The resolution of the endoscope detector 210 can be optionally 1920*1080.

[0049] The present invention can be used with a variety of different light sources, as long as the light source can emit narrow-band light of different wavelengths. It can be used with hyperspectral and hyperspectral light sources, broadening the application of hyperspectral and hyperspectral technologies, combining multispectral, hyperspectral and even hyperspectral technologies with endoscopes, making up for the shortcoming that light is difficult to apply in vivo due to limited penetration.

[0050] like Figure 4As shown, in a feasible light source design, the light source part 100 uses a design of a xenon lamp light source 110 + focusing lens group 120 + filter wheel 130. A focusing lens, a filter wheel 130, and a focusing lens are sequentially arranged in the light emission direction of the xenon lamp light source 110. The focusing lens can focus the light beam emitted by the xenon lamp with a diameter of about 60 mm to a beam diameter of 4 mm, and focus it on the light incident surface of the light guide channel. At the same time, the filter wheel is provided with a through hole for transmitting white light and a plurality of filters of different wavelengths. The filter can select the required narrowband waves from the wide spectrum of light emitted by the xenon lamp, thereby achieving the purpose of narrowband imaging. When in use, select a suitable filter wheel according to the needs. As shown in FIG. Figure 5 As shown, in another feasible light source design, a xenon lamp light source 110+focusing lens group 120+tunable liquid crystal filter LCTF140 design is used. Compared with the filter wheel, LCTF has a larger number of selectable wavelengths and is more controllable. These two feasible light source design schemes can be used as examples of multi-spectrum and hyperspectral light sources, respectively. Of course, the present invention does not limit the specific parameters and optical path structure of the light source. As long as it can emit white light and narrow-band light of different wavelengths, it can be used as a light source, such as a supercontinuum light source. In addition to the two feasible schemes listed above, LEDs, spectrometers and other instruments can also be used as components of the light source.

[0051] The data acquisition part 300 includes an acquisition card and a storage card. The acquisition card is used to receive the optical signal of the endoscope detector 210 and the imaging mode signal of the light source control unit, and process to obtain a tissue image; the storage card is used to store the tissue image obtained by the acquisition card; the acquisition card obtains an image label according to the imaging mode signal fed back by the light source control unit, processes the image according to the received optical signal of the endoscope detector 210, and adds an image label to the image to obtain a tissue image, which can be used for subsequent processing of machine learning algorithms to reconstruct different functional images. For example, the image obtained in the narrowband mode can be used to determine the blood oxygen saturation, and the image obtained in the fluorescence mode can be used to determine the photosensitizer dose before and after photodynamic therapy.

[0052] The data acquisition part uses an integrated acquisition card and memory card, which is compact and equipped with an operating handle and a UDB peripheral connection slot. It can be connected to an external USB to store images, making it easy to operate. The acquisition card has a real-time imaging function and can be connected to an external monitor for real-time endoscopic image display. At the same time, the image can be frozen through the acquisition card operating handle. After freezing, the image can be stored on an external USB device through a memory card.

[0053] The optical path of the present invention has been simulated and verified by Zemax software. The part of the optical path that extends into the body is simple, small in size, and has a wide range of application scenarios. It can be used in the digestive tract and colorectum of small and medium-sized animals.

[0054] The working process of the system of the present invention is as follows: if it is a white light imaging mode, the white light emitted by the light source part 100 is irradiated on the biological tissue to be inspected through the light guide channel, and the reflected light of the biological tissue to be inspected is detected by the endoscope detector to obtain a white light image, which is transmitted to the data acquisition part and displayed by the display. The white light image can provide the doctor with an intuitive feeling of the surgical site; if it is a narrow-band imaging mode, the narrow-band light with a wavelength that is not equal to the notch wavelength of the notch plate emitted by the light source part 100 is irradiated on the biological tissue to be inspected through the light guide channel, and the reflected light of the biological tissue to be inspected is collected by the endoscope detector in the endoscope part 200, and the endoscope detector is connected to the data acquisition part 300, so that the narrow-band image can be displayed in real time. The narrow-band images stored in the USB peripheral can generate multi-spectral or hyperspectral data sets, which can be combined with corresponding algorithms to determine the blood oxygen concentration; if it is a fluorescence imaging mode, the narrow-band light (also the photosensitizer excitation light) with a wavelength equal to the notch wavelength of the notch plate emitted by the light source part 100 is irradiated on the biological tissue to be inspected through the light guide channel, and the reflected light and fluorescence of the biological tissue to be inspected are irradiated to the endoscope part 200 together. After the reflected light is filtered out by the corresponding notch plate, the fluorescence is collected by the endoscope detector in the endoscope part 200. The endoscope detector is connected to the data acquisition part 300, and the image can be displayed and stored in real time. The fluorescence mode can be used to detect the photosensitizer concentration in the surgical site. The three modes of the endoscope do not need to be switched manually. They only need to be distinguished according to the wavelength of the illumination light used. The wavelength of the illumination light of the light source part is controlled in time through the program. When the non-fluorescence excitation band is selected, the obtained image is marked as a narrow-band image of the corresponding band, which corresponds to the narrow-band imaging mode; when the fluorescence excitation band that matches the notch wavelength of the notch plate is selected, because the excitation band is filtered out by the notch plate, a fluorescence image is obtained, which corresponds to the fluorescence mode. In short, it is only necessary to ensure that the wavelength of the excitation narrow-band light in the fluorescence mode is exactly the notch wavelength of the notch plate. The flowchart of the timing selection of the three modes is shown below. Figure 6 shown.

[0055] It should be noted that the present invention does not limit the wavelength of narrow-band light, and the specific wavelength parameters need to be selected according to the optical properties of the biological tissue being tested. Since the original intention of the present invention is to measure the oxygen content and photosensitizer concentration during photodynamic therapy, the narrow-band light is preferably one whose central wavelength is near the absorption peak of blood oxygenated hemoglobin and deoxygenated hemoglobin, and the wavelength of the fluorescent excitation light is preferably the excitation wavelength of the common photosensitizer in photodynamic therapy.

Claims

1. A white light-narrow band-fluorescence integrated endoscope, characterized in that: include: A light source part (100), a light source control unit and an endoscope part (200); The light source part (100) is used to provide illumination light for irradiating the biological tissue to be inspected, wherein the types of illumination light include white light and narrow-band light of multiple wavelengths, and the narrow-band light includes fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be inspected to generate fluorescence; The light source control unit is used to control the type of illumination light provided by the light source part (100); The endoscope part (200) comprises an endoscope detector (210) and a notch plate (230); the endoscope detector (210) is used to receive reflected light after the illumination light provided by the light source part (100) is reflected by the biological tissue to be inspected and fluorescence generated by the photosensitizer on the biological tissue to be inspected being excited by the fluorescence excitation light provided by the light source part (100), to obtain a light signal, and both the reflected light and the fluorescence enter the endoscope detector (210) through the notch plate (230); the notch wavelength of the notch plate (230) is equal to the wavelength of the fluorescence excitation light; The light source part (100) comprises a xenon light source (110), a focusing lens group (120) and a filter wheel (130); the focusing lens group (120) comprises a first focusing lens and a second focusing lens; the first focusing lens, the filter wheel (130) and the second focusing lens are sequentially arranged in the direction of light emitted by the xenon light source (110); or the light source part (100) comprises a xenon light source (110), a focusing lens group (120) and a tunable liquid crystal filter LCTF; the focusing lens group (120) comprises a first focusing lens and a second focusing lens; the first focusing lens, the tunable liquid crystal filter LCTF and the second focusing lens are sequentially arranged in the direction of light emitted by the xenon light source (110).

2. The white light-narrow band-fluorescence integrated endoscope according to claim 1, characterized in that: The endoscope portion (200) further comprises a holder (240), an endoscope detector channel (243) is provided inside the holder (240), an endoscope detector (210) is installed in the endoscope detector channel (243), a groove (244) is provided on an end surface of one end of the holder (240), the groove (244) is coaxial with and communicates with the endoscope detector channel (243), and a notch plate (230) is installed in the groove (244).

3. The white light-narrow band-fluorescence integrated endoscope according to claim 2, characterized in that: The holder (240) is provided with a light beam guiding channel, the axis of which is parallel to the axis of the endoscope detector channel (243); the illumination light provided by the light source part (100) is irradiated onto the biological tissue to be inspected through the light beam guiding channel.

4. The white light-narrow band-fluorescence integrated endoscope according to claim 3, characterized in that: Two light beam guiding channels are provided, and the two light beam guiding channels are symmetrical with respect to the endoscope detector channel (243).

5. The white light-narrow band-fluorescence integrated endoscope according to claim 1, characterized in that: It also includes a data acquisition part (300), which includes an acquisition card and a storage card. The acquisition card is used to receive the light signal of the endoscopic detector (210) and the imaging mode signal of the light source control unit, and process to obtain a tissue image; the storage card is used to store the tissue image obtained by the acquisition card.

6. The white light-narrow band-fluorescence integrated endoscope according to claim 5, characterized in that: It also includes a display, which is used to display the tissue image obtained by the acquisition part (300) in real time.

Images