Sample Chamber Measurement Method, Device, Computer Equipment and Storage Medium

Abstract

The present invention relates to a sampling chamber measurement method, device, computer equipment and storage medium. The measurement device includes a chamber body and a sampling needle with a part disposed in the chamber body; it also includes a light source and a detector. The present invention also provides a sampling chamber measurement method, including steps: calculating the initial sample volume; calculating the sample volume at the current moment; calculating the current sample running speed; calculating the number of cells that can be sorted; calculating the total number of cells to be sorted theoretically. By setting the light source and the detector, the detection light emitted by the light source undergoes refraction or scattering after passing through the container and the sample. According to the state of the sample, detection light with different signal intensities can be obtained. Based on the data such as the detection light with different signal intensities, the number of detectors, and the container volume, the initial sample volume, the sample volume at the current moment, the current sample running speed, the remaining sample running time, the number of cells that can be sorted, and the total number of cells to be sorted theoretically can be calculated in sequence.

Description

Technical Field

[0001] The present invention relates to the field of cell analysis and sorting equipment, and particularly to a method, device, computer equipment and storage medium for measuring the total number of sorted cells and the sample volume in a sample injection chamber. Background Art

[0002] A flow cytometer is a device for automatically analyzing and sorting cells. It can quickly measure, store, and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in a liquid, and can sort out a specified cell subset according to a preselected parameter range.

[0003] Currently, due to the relatively high internal pressure in the sample injection chamber of existing flow cytometers, the current sample remaining amount (i.e., the sample volume) can only be observed visually. This visual observation method is not precise enough. It can neither accurately measure the sample volume in the sample injection chamber of the cytometer nor estimate the number of target cells to be obtained. Summary of the Invention

[0004] Aiming at the above-mentioned disadvantages of the prior art, the purpose of the present invention is to provide a sample injection chamber measurement method, device, computer equipment and storage medium to solve one or more problems in the prior art.

[0005] To achieve the above purpose, the technical solution of the present invention is as follows:

[0006] The sample injection chamber measurement method includes the following steps:

[0007] Calculate the initial sample volume;

[0008] Calculate the sample volume at the current moment;

[0009] Calculate the current sample running speed;

[0010] Calculate the number of cells that can be sorted;

[0011] Calculate the total number of theoretically sorted cells.

[0012] Further, the calculation of the initial sample volume includes the following steps:

[0013] Obtain the number of detectors for the first time;

[0014] Obtain the container volume;

[0015] Pour the sample into the container;

[0016] Based on the following formula, obtain the initial sample volume:

[0017] Initial sample volume = container volume × (total number of detectors - number of detectors with the first intensity signal) / total number of detectors.

[0018] Further, the calculation of the sample volume at the current moment includes the following steps:

[0019] Statistical sample running time;

[0020] Obtain the number of detectors for the second time;

[0021] Obtain the sample volume at the current moment based on the following formula:

[0022] Sample volume at the current moment = container volume × (total number of detectors - detectors with first intensity signal) / total number of detectors.

[0023] Further, obtain the current sample running speed based on the following formula: current sample running speed = container volume / (total number of detectors × running time).

[0024] Further, obtain the remaining sample running time based on the following formula: remaining sample running time = sample volume at the current moment / current sample running speed.

[0025] Further, obtain the number of sortable cells based on the following formula: number of sortable cells = (number of cells sorted / running time) × remaining sample running time.

[0026] Further, obtain the total number of theoretically sorted cells based on the following formula: total number of theoretically sorted cells = number of cells sorted + number of sortable cells.

[0027] Further, the sample inlet chamber measurement method further includes calculating the cell concentration, and obtaining the cell concentration based on the following formula: cell concentration = number of detected cells / (initial sample volume - sample volume at the current moment).

[0028] Further, the sample inlet chamber measurement method further includes judging the sample running result, and the steps of judging the sample running result are as follows:

[0029] Obtain the average value of the first signal intensity count;

[0030] Obtain the average value of the second signal intensity count;

[0031] Compare the average value of the first signal intensity count and the average value of the second signal intensity count; if the average value of the first signal intensity count is equal to the average value of the second signal intensity count, execute the first result; if the average value of the first signal intensity count is less than the average value of the first signal intensity count, execute the second result; if the average value of the first signal intensity count is greater than the average value of the second signal intensity count, execute the third result.

[0032] Correspondingly, the present invention further provides a sample inlet chamber measurement device, and the sample inlet chamber measurement device includes

[0033] A storage body and a sampling needle with a part disposed on the storage body;

[0034] A light source, which is disposed on a part inside the storage body and is used for emitting detection light;

[0035] A detector, which is disposed on another part inside the storage body and is used for receiving the detection light.

[0036] Further, the light source includes a first mounting portion and at least two emission units connected to the first mounting portion, and the detector includes a second mounting portion and at least two receiving units connected to the second mounting portion.

[0037] Further, the number of the emission units is the same as or different from the number of the receiving units.

[0038] Correspondingly, the present invention further provides a computer device, including a memory, a processor, and a computer program stored on the memory and executable on the processor. When the processor executes the computer program, a sampling chamber measurement method is implemented.

[0039] Correspondingly, the present invention further provides a computer-readable storage medium, on which a computer program is stored. When the computer program is executed by a processor, a sampling chamber measurement method is implemented.

[0040] Compared with the prior art, the beneficial technical effects of the present invention are as follows:

[0041] (1) By disposing a light source and a detector in the storage body, the detection light emitted by the light source is refracted or scattered after passing through the container and the sample. According to the state of the sample, detection light with different signal intensities can be obtained. The initial sample volume, the sample volume at the current moment, the current sample running speed, the remaining sample running time, the number of cells that can be sorted, and the total number of cells to be sorted theoretically can be calculated in sequence through the above data such as the detection light with different signal intensities, the number of detectors, and the container volume.

[0042] (2) Further, by calculating the running speed of the sample and the sample volume at the current moment, the monitoring of the sample volume inside the container can be realized, and the end time of the sample injection can be calculated according to the remaining sample running time. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 Shows a schematic structural diagram of the sampling chamber measurement device according to an embodiment of the present invention.

[0044] Figure 2 Shows a cross-sectional structural diagram between the sampling chamber and the light source and the detector in the sampling chamber measurement method, device, computer device, and storage medium according to an embodiment of the present invention.

[0045] Figure 3 Shows a top view of the sample injection chamber, light source, and detector in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0046] Figure 4 Shows a schematic diagram of the light source passing through a sample tube containing a relatively large number of samples in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0047] Figure 5 Shows a schematic diagram of the light source passing through a sample tube without samples in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0048] Figure 6 Shows a schematic diagram of the light source passing through a sample tube with precipitated samples in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0049] Figure 7 Shows a schematic diagram of the principle of obtaining a first electrical signal when the light source passes through a sample tube without samples in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0050] Figure 8 Shows a schematic diagram of the principle of obtaining a second electrical signal when the light source passes through a sample tube containing partial samples in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0051] Figure 9 Shows a schematic diagram of the principle of obtaining a third electrical signal when the light source passes through a sample tube with precipitated samples in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0052] Figure 10 Shows a schematic diagram of the process of calculating the total number of cells to be sorted theoretically in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0053] Figure 11 Shows a schematic diagram of the process of calculating the initial sample volume in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0054] Figure 12 Shows a schematic diagram of the process of calculating the sample volume at the current moment in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0055] Figure 13 Shows a schematic diagram of the process of calculating the current sample running speed in the sample injection chamber measurement method, apparatus, computer device, and storage medium according to an embodiment of the present invention.

[0056] Figure 14 The figure shows a schematic flowchart of calculating the cell concentration in the sample injection chamber measurement method, device, computer device, and storage medium according to an embodiment of the present invention.

[0057] Figure 15 The figure shows a schematic flowchart of determining the sample operation result in the sample injection chamber measurement method, device, computer device, and storage medium according to an embodiment of the present invention.

[0058] Figure 16 The figure shows a schematic internal structure diagram of a computer device in the sample injection chamber measurement method, device, computer device, and storage medium according to an embodiment of the present invention.

[0059] Reference numerals in the drawings: 1, chamber body; 2, sampling needle; 3, light source; 300, first mounting portion; 301, emitting unit; 4, sample; 5, sample tube; 6, first detection light; 600, first reflected light; 601, second reflected light; 602, third reflected light; 7, second detection light; 700, first part of the second detection light; 701, second part of the second detection light; 702, third part of the second detection light; 8, detector; 800, second mounting portion; 801, receiving unit. Detailed implementation manners

[0060] In order to make the objectives, technical solutions, and advantages of the present invention clearer and more understandable, the sample injection chamber measurement method, device, computer device, and storage medium proposed by the present invention will be further described in detail below with reference to the accompanying drawings and specific implementation manners. According to the following description, the advantages and features of the present invention will be clearer. It should be noted that the accompanying drawings are in a very simplified form and all use non-precise scales, only for the purpose of facilitating and clearly assisting in explaining the implementation manners of the present invention. In order to make the objectives, features, and advantages of the present invention more obvious and understandable, please refer to the accompanying drawings. It should be noted that the structures, scales, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification for those skilled in the art to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention. Therefore, they do not have technical substance significance. Any modification of the structure, change of the proportional relationship, or adjustment of the size, without affecting the effects that the present invention can produce and the objectives that can be achieved, should still fall within the scope covered by the technical content disclosed by the present invention.

[0061] Please refer to Figure 1 , a sample injection chamber measurement device, including a chamber body 1 and a sampling needle 2, a part of the sampling needle 2 extends axially into the chamber body 1, and another part of the sampling needle 2 extends out of the chamber body 1.

[0062] Please refer to Figure 1 , Figure 2 and Figure 3, the measuring device further includes a light source 3. Specifically, the light source 3 is disposed on a part of the inner wall of the chamber body 1 (not shown in the figure), and the light source 3 is used to emit detection light that can penetrate the sample tube 4 and the sampling needle 2. Figure 1 The detection light described therein is denoted as the first detection light 6, and the first detection light 6 only represents the light that is emitted and contacts the sample tube 5 or the sample 4 (which can also be understood as unrefracted light). Preferably, in the measuring device according to Embodiment 1 of the present invention, the light source 3 is preferably an infrared laser. Similarly, the measuring device further includes a detector 8, and the detector 8 is disposed on another part of the inner wall of the chamber body 1. The detector is used to receive the second detection light 7, and the second detection light 7 is the light formed by the first detection light 6 penetrating the sample 4 and / or penetrating the sample tube 5 and refracting.

[0063] Further, please continue to refer to Figure 2 and Figure 3 , the light source 3 includes a first mounting portion 300 and at least two emitting units 301 connected to the first mounting portion 300. Similarly, the detector 8 includes a second mounting portion 800 and at least two receiving units 801 connected to the second mounting portion 800. Among them, the number of the emitting units 301 provided is the same as or different from the number of the receiving units 801 provided.

[0064] Please refer to Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 , Figure 9 , according to the different contents of the sample in the sample tube 5, the number of lights obtained by the detector 8 is also different.

[0065] Specifically, please refer to Figure 5 and Figure 7 , when the sample tube 5 does not contain the sample 4, a plurality of first detection lights 6 emitted by the emitting unit 301 penetrate the sample tube 5, and the first detection light 6 penetrates the tube wall of the sample tube 5 and refracts to form the second detection light 7. Specifically, in this embodiment, the second detection light 7 is denoted as the first part 700 of the second detection light. The first part 700 of the second detection light only passes through the empty sample tube 5 and the amount of refraction attenuation is small. The first part 700 of the second detection light irradiates the target surface of the receiving unit 801 in the corresponding detector 8, and the number of the first part 700 of the second detection light is the same as the number of the first detection lights 6 emitted by the emitting unit 301. At this time, the detector 8 generates a first intensity signal (or can also be called a strong (High) signal). Please refer to Figure 5 , generating the first intensity signal indicates that the number of the first detection lights 6 emitted by the emitting unit 301 is the same as the number of the second detection lights 7 formed by penetrating the sample tube 5.

[0066] Similarly, please refer to Figure 4 and Figure 8 When there is a large amount of sample 4 in the sample tube 5, the first detection light 6 above the liquid level of the sample 4 penetrates the tube wall of the sample tube 5 to form the first part 700 of the second detection light. In this state, the amount of attenuation of the second detection light 7 due to refraction is small, and after this part of the detection light is received by the detector 8, a first intensity signal is still generated. The difference is that the first detection light 6 below the liquid level of the sample 4 not only needs to penetrate the tube wall of the sample tube 5, but also needs to penetrate the sample 4. Therefore, the first detection light 6 passing through the sample tube 5 and the sample 4 is reflected to form the second part 701 of the second detection light. At the same time, a part of the first detection light 6 is refracted to form reflected light. For example, in the first embodiment, the first detection light 6 is refracted into the first reflected light 600 and the second reflected light 601, so that the amount of attenuation of the second part 701 of the second detection light due to refraction is relatively large, that is, only a small part of the second part 701 of the second detection light irradiates the target surface of the receiving unit 801 in the corresponding detector 8. At this time, the detector 8 converts to generate a second intensity signal (or it can also be called a medium intensity (Mid) signal). Please refer to Figure 8 This second intensity signal indicates that the number of the first detection lights 6 is greater than that of the second detection lights 7, and the number of the second part 701 of the second detection lights 7 is the same as the number of the reflected lights (that is, the first reflected light 600 and the second reflected light 601).

[0067] Please refer to Figure 6 and Figure 9 The first detection light 6 at the lowest position of the liquid level of the sample 4 forms the third part 702 of the second detection light after penetrating the sample tube 5 and the sample 4. A large amount of the first detection lights 6 are refracted or reflected by the cells / particles in the sample and cannot reach the target surface of the receiving unit 801 in the detector 8. Specifically, please refer to Figure 9 After refraction, the second detection light forms the third part 702 of the second detection light. Similarly, the first detection light 6 is reflected by the cells / particles in the sample to form the first reflected light 600, the second reflected light 601, and the third reflected light 602. In this case, the number of the first detection lights 6 is greater than that of the second detection lights 7, and finally the number of the refracted third part 702 of the second detection light is less than the number of the reflected lights (that is, the first reflected light 600, the second reflected light 601, and the third reflected light 602), that is, only the third part 702 of the second detection light can irradiate the target surface of the receiving unit 801 in the detector 8. At this time, the detector 8 converts to generate a third intensity signal (or it can also be called a weak signal (Low)).

[0068] Correspondingly, according to different sample driving methods, the sample injection chamber measuring device of the present invention can adopt two types: positive pressure injection and pump injection. Among them, positive pressure injection means injecting compressed air into the chamber body 1 and using the compressed air as the driving force to press the sample into the injection needle 2. When using pump injection, a pump is connected to the tail of the injection needle 2, and the power of the pump is used to drive the sample into the injection needle 2. Preferably, in the sample injection chamber measuring device described in the first embodiment of the present invention, the pump is preferably any one of a peristaltic pump or a syringe pump. Of course, in other embodiments of the present invention, the pump can also adopt any structure other than a peristaltic pump or a syringe pump, as long as it can realize driving the sample into the injection needle 2 by relying on power. The present invention does not make further limitations on this.

[0069] Correspondingly, in one embodiment, a computer device is provided. Please refer to Figure 16 , this computer device can be a terminal. The computer device includes a processor, a memory, a network interface, a display screen, and an input device connected through a system bus. Among them, the processor of the computer device is used to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the operation of the operating system and the computer program in the non-volatile storage medium. The network interface of the computer device is used to communicate with the server through a network connection. When the computer program is executed by the processor, it realizes a sample injection chamber measurement method. The display screen of the computer device can be a liquid crystal display screen or an electronic ink display screen. The input device of the computer device can be a touch layer covered on the display screen, or a button, a trackball, or a touchpad set on the outer shell of the computer device, or an external keyboard, a touchpad, or a mouse, etc.

[0070] Those skilled in the art can understand that Figure 16 the structure shown in

[0071] is only a block diagram of some structures related to the solution of the present application, and does not constitute a limitation on the computer device to which the solution of the present application is applied. The specific computer device may include more or fewer components than those shown in the figure, or combine some components, or have different component arrangements.

[0072] Correspondingly, the present invention further provides a measurement method for an injection chamber. The ultimate goal of the measurement method is to measure the total number of cells that can be sorted theoretically, the cell concentration, and the sample running speed that can be monitored, and the end time can also be estimated according to the remaining sample running time. The measurement method includes the following steps:

[0073] S1: Calculate the initial sample volume. The calculation of the initial sample volume includes the following steps:

[0074] S100: Obtain the number of signal detectors with different intensities for the first time. Please refer to Figure 4 、 Figure 10 and Figure 11 , in the injection chamber measurement method of the first embodiment, the total number of detectors 8 is counted as ten.

[0075] S101: Obtain the total volume of the container. Please refer to Figure 4 、 Figure 10 and Figure 11 , in the measurement method of this embodiment, the volume of the container, that is, the sample tube 5, is 5000 μL. Ten detectors 8 divide the 5000 μL sample tube 5 into ten 500 μL regions on average.

[0076] S102: Pour 3000 μL of sample 4 into the sample tube 5.

[0077] S103: After pouring sample 4, obtain the number of signal detectors with different intensities. Please continue to refer to Figure 4 and Figure 10 , please refer to Figure 4 、 Figure 10 and Figure 11 , among which, a total of four detectors 8 from top to bottom receive the first part 700 of the second detection light. After the four detectors 8 receive the first part 700 of the second detection light, a first intensity signal (also called a strong signal) is generated. Similarly, the remaining six detectors 8 receive the second part 701 of the second detection light and then generate a second intensity signal (also called a medium intensity signal).

[0078] S104: Obtain the initial sample volume according to the formula. Specifically, the calculation formula for the initial sample volume is as follows:

[0079] Initial sample volume = container volume × (number of detectors (总数) - number of detectors (第一强度信号) ) / total number of detectors = 5000 μL × (10 - 4) / 10 = 3000 μL.

[0080] Of course, in other embodiments of the present invention, the number of detectors 8 is not limited to the ten described in this embodiment. The number of detectors receiving the first part 700 of the second detection light and the number of detectors receiving the second part 701 of the second detection light may also be different from those in this embodiment. In this regard, the present invention will not be further elaborated.

[0081] S2: Calculate the sample volume at the current moment. Please refer to Figure 12 , and the calculation of the sample volume at the current moment includes the following steps:

[0082] S200: Statistically analyze the sample running time. Specifically, the sample running time is 10 min.

[0083] S201: Obtain the number of detectors with different intensity signals for the second time. Among them, a total of five detectors 8 from top to bottom received the first part 700 of the second detection light. After the above five detectors 8 received the first part 700 of the second detection light, a first intensity signal (also called a strong signal) was generated. Similarly, after the remaining five detectors 8 received the second part 701 of the second detection light, a second intensity signal (also called a medium intensity signal) was generated. It can be seen that 500 μL of the sample has been run. At this time, the number of sorted cells is 5,000, and the number of detected cells is 50,000.

[0084] S202: Obtain the current sample volume according to the formula. Specifically, the calculation formula for the sample volume at the current moment is as follows:

[0085] Sample volume at the current moment = container volume × (number of detectors (总数) - number of detectors (第一强度信号) ) / total number of detectors = 5000 μL × (10 - 5) / 10 = 2500 μL.

[0086] Correspondingly, between step S200 and step S201, it is also necessary to judge the sample running result, that is, the mixing strength of the sample inside the sample tube 5 after running for 10 min. Whether the sample is mixed or not determines the magnitude of the refractive attenuation of the second detection light 7, and even affects the statistical analysis of the detector intensity signal, thus affecting the operation of subsequent data results. The judgment of the sample running result includes the following steps:

[0087] Please refer to Figure 15 , S203: Obtain the average value of the first signal intensity count. Specifically, the signal output of the detector 8 is a voltage signal. Please refer to Figure 4, When the sample starts to run, it is uniform. Therefore, the electrical signals below the liquid level of sample 4 are all equal. The detector 8 receives the second part 701 of the second detection light and outputs a second intensity signal of 3V. Similarly, the electrical signals on the liquid surface of sample 4 are also equal. The detector 8 receives the first part 700 of the second detection light, and the detector 8 outputs a first intensity signal of 5V.

[0088] S204: Obtain the average value of the second signal intensity count. Specifically, please refer to Figure 6 , After running for a period of time, the cells precipitate due to gravity and fall to the bottom of the sample tube 5. At this time, the electrical signal will be different from the electrical signal in step S203. When the detector receives the third part 702 of the second detection light, the detector 8 outputs a third intensity signal of 1V.

[0089] S205: Compare the average value of the first signal intensity count with the average value of the second signal intensity count. If the average value of the first signal intensity count is equal to the average value of the second signal intensity count, the sample has not settled, and at this time, execute the first result, that is, the sample is not mixed evenly. If the average value of the first signal intensity count is less than the average value of the first signal intensity count, execute partial sedimentation of the sample, and at this time, start low-speed mixing; if the average value of the first signal intensity count is greater than the average value of the second signal intensity count, at this time, execute serious sedimentation of the sample and start high-speed mixing; stop mixing until the detector below the liquid level outputs a signal of 3V. If the cells precipitate in the sample, it will cause blockage of the sampling tube, and the purpose of mixing is to avoid sample precipitation and avoid blockage of the sampling tube.

[0090] S3: Please refer to Figure 13 , Calculate the current sample running speed. The calculation formula for the sample running speed is as follows:

[0091] Current sample running speed = container volume / (total number of detectors × running time) = 5000 μL / (10 × 10) = 50 μL / min.

[0092] S4: Calculate the remaining sample running time. The calculation formula for the remaining sample calculation time is as follows:

[0093] Remaining sample running time = current sample volume / current sample running speed = 2500 μL / 50 μL / min = 50 min. At this time, the user can estimate the end time of sample injection based on the current time. The end time of this sample injection = current time + remaining sample running time. For example, if it is 9:30 now and the remaining sample running time is 50 min, so the user can estimate that the end time of sample injection without changing the injection speed is 10:20. Thus, it is convenient for the user to reasonably arrange other work matters after estimating the end time of sample injection. s

[0094] S5: Next, calculate the number of sortable cells. The calculation formula for the number of sortable cells is as follows:

[0095] Number of sortable cells = (Number of sorted cells / Running time) × Remaining sample running time = (5000 / 10 min) × 50 min = 25000.

[0096] S6: Please refer to Figure 10 and calculate the total number of theoretically sorted cells. The calculation formula for the total number of theoretically sorted cells is as follows:

[0097] Total number of theoretically sorted cells = Number of sorted cells + Number of sortable cells = 5000 + 25000 = 30000.

[0098] Therefore, using the measurement method of the sample chamber measuring device described in this embodiment, the total number of cells that can be theoretically sorted by the sample chamber measuring device can be calculated as 30000.

[0099] Furthermore, please refer to Figure 14 and the cell concentration can also be calculated using the above measurement method. The calculation formula for the cell concentration is as follows:

[0100] Cell concentration = Number of detected cells / (Initial sample volume - Current sample volume) = 50000 / (3000 μL - 2500 μL) = 100 / μL.

[0101] According to the above measurement method, not only the total number of theoretically sorted cells can be obtained, but also the current sample volume, current sample running speed, remaining sample running time, number of sortable cells, and cell concentration can be calculated. It does not require visual inspection of the sample tube 5 and can be directly calculated by software. The calculation results can be finally stored in the memory and retrieved by the processor, and can also be displayed on an external display unit such as a monitor, which is convenient for the user to view. Similarly, the above results such as the current sample volume, current sample running speed, remaining sample running time, number of sortable cells, and cell concentration can also be uploaded to the cloud memory, and the calculation results can be obtained from the cloud memory remotely.

[0102] The technical features of the above embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0103] The above-described embodiments merely represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. Sampling chamber measurement method, characterized in that It includes the following steps: Calculate the initial sample volume; Calculate the sample volume at the current moment; Calculate the current sample running speed; Calculate the number of cells that can be sorted; Calculate the total number of cells to be sorted theoretically; The sample injection chamber measurement method further includes judging the sample running result, and the steps of judging the sample running result are as follows: Obtain the average value of the first intensity signal count; Obtain the average value of the second intensity signal count; Compare the average value of the first intensity signal count and the average value of the second intensity signal count; if the average value of the first intensity signal count is equal to the average value of the second intensity signal count, execute the first result, that is, the sample is not mixed evenly; if the average value of the first intensity signal count is less than the average value of the second intensity signal count, execute the second result, and the second result is to perform partial sedimentation of the sample and start low-speed mixing; if the average value of the first intensity signal count is greater than the average value of the second intensity signal count, execute the third result, and the third result is to start high-speed mixing; Wherein the sample injection chamber measurement method is applied to a sample injection chamber measurement device, and the device includes a chamber body; A light source, the light source is arranged in a part inside the chamber body and is used for emitting detection light; A detector, the detector is arranged in another part inside the chamber body and is used for receiving the detection light; The detection light includes a first detection light, and the light refracted by the first detection light penetrating the sample or the sample tube is a second detection light; a first part of the second detection light is received by the detector, causing the detector to generate a first intensity signal; A second part of the second detection light is received by the detector, causing the detector to generate a second intensity signal; The first intensity signal indicates that the number of the first detection lights emitted by the emission unit is the same as the number of the second detection lights formed by penetrating the sample tube; the second intensity signal indicates that the number of the first detection lights is greater than the number of the second detection lights, and the number of the second part of the second detection lights in the second detection lights is the same as the number of the reflected lights.

2. The sample introduction chamber measurement method according to claim 1, characterized in that: The calculation of the initial sample volume includes the following steps: Obtain the number of detectors for the first time; Obtain the container volume; Pour the sample into the container; Obtain the initial sample volume based on the following formula: Initial sample volume = container volume × (detector （总数） -detector （第一强度信号） ) / total number of detectors.

3. The sample injection chamber measurement method according to claim 2, characterized in that: The calculation of the sample volume at the current moment includes the following steps: Count the sample running time; Obtain the number of detectors for the second time; Obtain the sample volume at the current moment based on the following formula: Current moment sample volume = container volume × (detector （总数） - detector （第一强度信号） ) / total number of detectors.

4. The sample injection chamber measurement method according to claim 3, characterized in that: Obtain the current sample running speed based on the following formula: current sample running speed = container volume / (total number of detectors × running time).

5. The sample injection chamber measurement method according to claim 4, characterized in that: Obtain the remaining sample running time based on the following formula: remaining sample running time = sample volume at the current moment / current sample running speed.

6. The sample injection chamber measurement method according to claim 5, characterized in that: Obtain the number of cells that can be sorted based on the following formula: number of cells that can be sorted = (number of cells sorted / running time) × remaining sample running time.

7. The sample injection chamber measurement method according to claim 6, characterized in that: Obtain the total number of cells to be sorted theoretically based on the following formula: total number of cells to be sorted theoretically = number of cells sorted + number of cells that can be sorted.

8. The sample injection chamber measurement method according to claim 3, characterized in that: The sample injection chamber measurement method further includes calculating the cell concentration, and obtaining the cell concentration based on the following formula: cell concentration = number of cells detected / (initial sample volume - sample volume at the current moment).

9. Sampling chamber measuring device, characterized in that: The sample injection chamber measuring device performs measurement using the sample injection chamber measuring method according to any one of claims 1 to 8, and the sample injection chamber measuring device includes a chamber body and a sample injection needle with a part disposed in the chamber body; a light source, which is disposed in a part inside the chamber body and is used for emitting detection light; a detector, which is disposed in another part inside the chamber body and is used for receiving the detection light.

10. The sample injection chamber measuring device according to claim 9, characterized in that: The light source includes a first mounting part and at least two emission units connected to the first mounting part, and the detector includes a second mounting part and at least two receiving units connected to the second mounting part.

11. The sample introduction chamber measuring device according to claim 10, characterized in that: The number of the emission units is the same as or different from the number of the receiving units.

12. A computer device, comprising a memory, a processor, and a computer program stored on the memory and executable on the processor, characterized in that: When the processor executes the computer program, the method according to any one of claims 1 to 8 is implemented.

13. A computer-readable storage medium having a computer program stored thereon, characterized in that, When the computer program is executed by the processor, the method according to any one of claims 1 to 8 is implemented.

Images