An animal tissue sampling device

Abstract

The application provides an animal tissue sampling device and belongs to the technical field of cell culture. The technical scheme is as follows: a base and a sampling mechanism arranged on the base, the base is provided with an operation sleeve through a pair of inverted L-shaped supporting rods, the sampling mechanism is movably arranged in the operation sleeve, the sampling mechanism comprises a fixing frame movably arranged in the operation sleeve, the fixing frame is provided with an outer sampling sleeve, the fixing frame comprises a U-shaped handle, the U-shaped handle is respectively provided with a first fixing cylinder and a second fixing cylinder at both ends, and the outer sampling sleeve is arranged between the first fixing cylinder and the second fixing cylinder. The application has the advantages of simple structure, convenient use, fast sampling, great guarantee of sample pollution, and improved efficiency and quality of scientific research.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, and more particularly to an animal tissue sampling device. Background Technology

[0002] With the continuous improvement of people's living standards, the demand for meat in daily life has increased. Meat is rich in various nutrients. However, livestock such as pigs and donkeys, which are edible for humans, grow relatively slowly. Even under proper feeding and management conditions during the fattening period, they only gain an average of slightly more than half a kilogram per day. Since the weight gain of organisms is achieved through cell proliferation, it is necessary to establish a livestock muscle cell culture system to study the mechanism of muscle cell proliferation and provide a scientific theoretical basis for improving the daily weight gain of livestock.

[0003] Establishing a livestock muscle cell culture system first requires taking a small amount of muscle tissue from the animal. A small amount of tissue from the plumpest part of the muscle is cut with scissors and placed in a sample bottle containing physiological saline or PBS with forceps and brought back to the laboratory. Since most slaughterhouses are small workshops with relatively simple environmental conditions, and tissue sampling is an open process, aseptic operation cannot be guaranteed. Therefore, the small amount of muscle tissue cut may have already been contaminated with bacteria or other contaminants. When primary muscle cell culture is carried out, bacterial growth is almost inevitable, resulting in serious waste of scientific research. Summary of the Invention

[0004] The purpose of this invention is to provide an animal tissue sampling device that is simple in structure, easy to use, can quickly sample, and greatly ensures that the sample is not contaminated, thereby improving the efficiency and quality of scientific research.

[0005] This invention is achieved through the following measures:

[0006] An animal tissue sampling device, characterized in that it includes a base and a sampling mechanism disposed on the base;

[0007] An operating sleeve is provided on the base via a pair of inverted L-shaped support rods, and the sampling mechanism is movably disposed in the operating sleeve.

[0008] The sampling mechanism includes a fixed frame movably inserted into the operating sleeve. An outer sampling sleeve is provided on the fixed frame. The fixed frame includes a U-shaped handle. A first fixed cylinder and a second fixed cylinder are respectively provided at both ends of the U-shaped handle. The outer sampling sleeve is provided between the first fixed cylinder and the second fixed cylinder. Both ends of the outer sampling sleeve are provided with annular grooves. Annular limiting plates are provided in both annular grooves. A first annular slot is provided on one end face of the first fixed cylinder and the second fixed cylinder. The annular limiting plates are respectively provided in the first annular slots on both sides.

[0009] The invention also has the following specific features:

[0010] The first fixed cylinder has a first annular cutter head at the end away from the outer sampling sleeve, and the second fixed cylinder has an annular airbag at the inner side of the end near the outer sampling sleeve.

[0011] A cross strut is provided on the inner side of the end of the second fixed cylinder away from the outer sampling sleeve, and an operating rod is provided on the cross strut.

[0012] A pair of fixing plates are symmetrically arranged on the side walls at both ends of the outer sampling sleeve. The inner side of the fixing plates on both sides is provided with a slot that penetrates the interior of the annular sliding groove. A fixing seat is provided between the slots on both sides and on the side wall of the outer sampling sleeve. A guide rod is fixedly installed on the fixing seat. The two ends of the guide rod are respectively set on the fixing plates on both sides.

[0013] A pair of annular limiting inserts are provided with guide sleeves near their ends. One end of the guide sleeve is provided on the annular limiting insert, and the other end of the guide sleeve extends out of the slot and is provided around the guide rod. A spring is provided between the guide sleeves on both sides and the fixed seat, and the spring is provided around the guide rod.

[0014] Preferably, pressure plates are provided on both sides of the guide sleeve.

[0015] An inner sampling sleeve is provided on the inner side of the outer sampling sleeve by several support rods. A second annular cutter head is provided on both ends of the inner sampling sleeve, and the ends of the second annular cutter head on both sides are flush with the ends of the outer sampling sleeve.

[0016] The first fixed cylinder has a first limiting strip symmetrically arranged on both outer walls, and the second fixed cylinder has a pair of second limiting strips arranged on both outer walls corresponding to the positions of the first limiting strips;

[0017] The inner side of the operating sleeve is provided with a vertically penetrating limiting groove corresponding to the positions of the first limiting strips on both sides, and the inner side wall of the operating sleeve is provided with corresponding receiving grooves corresponding to the positions of the pressure plate and the U-shaped handle.

[0018] The base is provided with a second annular slot, which is concentrically arranged with the operating sleeve.

[0019] Limit slots are formed on the outer periphery of the second annular slot and on the base corresponding to the positions of a pair of the first limiting strips;

[0020] An airbag inlet pipe communicating with the inside of the annular airbag is provided in the first limiting strip on one side. The end of the airbag inlet pipe is set away from the outer sampling sleeve. The end of the airbag inlet pipe is flush with the end of the first limiting strip. A touch plate is provided at the end of the first limiting strip away from the outer sampling sleeve on the other side.

[0021] The annular airbag is also provided with an airbag exhaust pipe, which passes through the first fixed cylinder;

[0022] An air inlet tube is provided at the bottom of the limiting slot on one side, corresponding to the position of the airbag inlet tube. An air pump is also provided on the base. A sensor for use with the air pump is provided at the bottom of the limiting slot on the other side. The air inlet tube is connected to the air outlet of the air pump.

[0023] Both the airbag inlet pipe and the airbag outlet pipe are equipped with one-way valves.

[0024] The one-way valve on the airbag inlet pipe ensures that gas can only enter and not exit the annular airbag, and the one-way valve on the airbag exhaust pipe ensures that gas can only exit and not enter the annular airbag.

[0025] The method of using the sampling device includes the following steps:

[0026] S1. First, clean and disinfect the base and the sampling mechanism. Then, assemble the sampling mechanism by squeezing the pressure plates on both sides to bring the guide sleeves on both sides closer together, thereby storing the annular limiting inserts on both sides into the first annular slot. Then, place the outer sampling sleeve between the first fixed cylinder and the second fixed cylinder, and keep the guide rod and the U-shaped handle opposite to each other. Then, loosen the pressure plates so that the ends of the annular limiting inserts on both sides enter the first annular slots of the first fixed cylinder and the second fixed cylinder to complete the assembly. At the same time, squeeze and vent the gas in the annular airbag.

[0027] S2. Cut open the skin of the muscle to be sampled, place the muscle directly below the operating sleeve, and turn on the power to the air pump. Insert the sampling mechanism into the operating sleeve and continuously press down on the sampling mechanism. The first annular cutter head can cut the muscle. The muscle enters the outer sampling sleeve and the second sampling sleeve along the first fixed sleeve. The muscle entering the outer sampling sleeve is cut by the second annular cutter head, and part of the muscle will enter the inner sampling sleeve. Continue to press down on the sampling mechanism until the first annular cutter head reaches the bottom of the first annular slot. When the first annular cutter head reaches the bottom of the first annular slot, the air inlet tube is fully inserted into the air bag inlet tube. At the same time, the touch plate touches the sensor, and the air pump works to inflate the annular air bag (the gas pumped in by the air pump in one operation is matched with the overall volume of the annular air bag). Finally, pull out the sampling mechanism. The inflated annular air bag can squeeze the muscle in the sampling mechanism to prevent the muscle from falling out.

[0028] S3. Bring the sampling mechanism after initial sampling to the laboratory, place the sampling mechanism horizontally on the table, squeeze the pressure plates on both sides to bring the guide sleeves on both sides closer together, thereby storing the annular limiting inserts on both sides into the first annular slot. Use a knife to cut along the gap between the outer sampling sleeve and the first and second fixed sleeves to separate the muscle inside the outer sampling sleeve. Finally, remove the muscle inside the inner sampling sleeve to complete the sampling.

[0029] The beneficial effects of this invention are: simple structure, convenient use, rapid sampling, and to a great extent ensure that the sample is not contaminated, thereby improving the efficiency and quality of scientific research. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the structure of an embodiment of the present invention.

[0031] Figure 2 This is a schematic diagram of the base structure in an embodiment of the present invention.

[0032] Figure 3 This is a schematic diagram of the sampling mechanism in an embodiment of the present invention.

[0033] Figure 4 This is a schematic diagram of the internal structure of the sampling mechanism in an embodiment of the present invention.

[0034] Figure 5 for Figure 4 A magnified view of part A in the middle.

[0035] Figure 6 This is a schematic diagram of the internal structure of the sampling mechanism in an embodiment of the present invention.

[0036] Figure 7 for Figure 6 A magnified view of part B in the middle.

[0037] Figure 8 This is a schematic diagram of the structure of the external sampling sleeve in an embodiment of the present invention.

[0038] Figure 9 This is a schematic diagram of the internal structure in use according to an embodiment of the present invention.

[0039] Figure 10 for Figure 9 A magnified view of part C in the middle.

[0040] The attached figures are labeled as follows: 1. Base; 101. Second annular slot; 102. Limiting slot; 2. Operating sleeve; 201. Limiting groove; 202. Receiving groove; 3. Sampling mechanism; 4. Inverted L-shaped support rod; 5. First fixed cylinder; 6. Outer sampling sleeve; 601. Groove; 602. Annular slide groove; 7. Second fixed cylinder; 8. First limiting strip; 9. Second limiting strip; 10. Cross support rod; 11. Operating rod; 12. First annular cutter head; 13. First annular slot; 14. Annular limiting insert plate; 15. Fixed plate; 16. Guide rod; 17. Guide sleeve; 18. Pressure plate; 19. Spring; 20. Fixed seat; 21. Support rod; 22. Inner sampling sleeve; 23. Second annular cutter head; 24. Annular airbag; 25. Airbag inlet pipe; 26. Inlet tube; 27. Sensor. Detailed Implementation

[0041] To clearly illustrate the technical features of this solution, the following detailed implementation method will be used to describe the solution.

[0042] See Figure 1-10 An animal tissue sampling device includes a base 1 and a sampling mechanism 3 disposed on the base 1;

[0043] An operating sleeve 2 is provided on the base 1 via a pair of inverted L-shaped support rods 4, and a sampling mechanism 3 is movably installed in the operating sleeve 2;

[0044] The sampling mechanism 3 includes a fixed frame that is movably inserted into the operating sleeve 2. An outer sampling sleeve 6 is provided on the fixed frame. The fixed frame includes a U-shaped handle. A first fixed cylinder 5 and a second fixed cylinder 7 are respectively provided at both ends of the U-shaped handle. The outer sampling sleeve 6 is provided between the first fixed cylinder 5 and the second fixed cylinder 7. Both ends of the outer sampling sleeve 6 are provided with annular grooves 602. Annular limiting plates 14 are provided in both annular grooves 602. A first annular slot 13 is provided on the opposite end face of the first fixed cylinder 5 and the second fixed cylinder 7. The annular limiting plates 14 are respectively provided in the first annular slots 13 on both sides.

[0045] The first fixed cylinder 5 is provided with a first annular cutter head 12 at the end away from the outer sampling sleeve 6, and the second fixed cylinder 7 is provided with an annular airbag 24 on the inner side of the end near the outer sampling sleeve 6.

[0046] A cross strut 10 is provided on the inner side of the end of the second fixed cylinder 7 away from the outer sampling sleeve 6, and an operating rod 11 is provided on the cross strut 10.

[0047] A pair of fixing plates 15 are symmetrically arranged on the side walls of both ends of the outer sampling sleeve 6. The inner side of the fixing plates 15 on both sides is provided with a slot 601 that penetrates the interior of the annular sliding groove 602. A fixing seat 20 is provided between the slots 601 on both sides and on the side wall of the outer sampling sleeve 6. A guide rod 16 is fixedly installed on the fixing seat 20. The two ends of the guide rod 16 are respectively set on the fixing plates 15 on both sides.

[0048] A pair of annular limiting plates 14 are provided with guide sleeves 17 near the end. One end of the guide sleeve 17 is provided on the annular limiting plate 14, and the other end of the guide sleeve 17 extends out of the slot 601 and is provided around the guide rod 16. Springs 19 are provided between the guide sleeves 17 on both sides and the fixed seat 20. The springs 19 are provided around the guide rod 16.

[0049] Preferably, pressure plates 18 are provided on both sides of the guide sleeve 17.

[0050] An inner sampling sleeve 22 is provided on the inner side of the outer sampling sleeve 6 by several support rods 21. A second annular cutter head 23 is provided on both ends of the inner sampling sleeve 22. The ends of the second annular cutter heads 23 on both sides are flush with the ends of the outer sampling sleeve 6.

[0051] The first fixed cylinder 5 has a first limiting strip 8 symmetrically arranged on both outer walls, and the second fixed cylinder 7 has a pair of second limiting strips 9 arranged on both outer walls corresponding to the positions of the first limiting strips 8;

[0052] The inner side of the operating sleeve 2 is provided with a vertically penetrating limiting groove 201 corresponding to the position of the first limiting strip 8 on both sides, and the inner side wall of the operating sleeve 2 is provided with corresponding receiving grooves 202 corresponding to the position of the pressure plate 18 and the U-shaped handle.

[0053] The base 1 is provided with a second annular slot 101, which is concentrically arranged with the operating sleeve 2.

[0054] Limit slots 102 are provided on the outer periphery of the second annular slot 101 and on the base 1 at positions corresponding to a pair of first limit bars 8;

[0055] An airbag inlet pipe 25 communicating with the inside of the annular airbag 24 is provided inside the first limiting strip 8 on one side. The end of the airbag inlet pipe 25 is set away from the outer sampling sleeve 6. The end of the airbag inlet pipe 25 is flush with the end of the first limiting strip 8. A touch plate is provided at the end of the first limiting strip 8 away from the outer sampling sleeve 6 on the other side.

[0056] The annular airbag 24 is also equipped with an airbag exhaust pipe, which passes through the first fixed cylinder 5;

[0057] An air inlet tube 26 is provided at the bottom of one side limit slot 102 corresponding to the position of the airbag inlet tube 25. An air pump is also provided on the base 1. A sensor 27 for use with the air pump is provided at the bottom of the other side limit slot 102. The air inlet tube 26 is connected to the air outlet of the air pump.

[0058] One-way valves are installed on both the airbag inlet pipe 25 and the airbag exhaust pipe;

[0059] The one-way valve on the airbag inlet pipe 25 ensures that gas can only enter and not exit the annular airbag 24, while the one-way valve on the airbag exhaust pipe ensures that gas can only exit and not enter the annular airbag 24.

[0060] The method of using the sampling device includes the following steps:

[0061] S1. First, clean and disinfect the base 1 and the sampling mechanism 3. Then assemble the sampling mechanism 3. Squeeze the pressure plates 18 on both sides to bring the guide sleeves 17 on both sides closer together, so that the annular limiting inserts 14 on both sides are stored in the annular slots 13. Then place the outer sampling sleeve 6 between the first fixed cylinder 5 and the second fixed cylinder 7, and keep the guide rod 16 and the U-shaped handle opposite each other. Then loosen the pressure plate 18 so that the ends of the annular limiting inserts 14 on both sides enter the annular slots 13 of the first fixed cylinder 5 and the second fixed cylinder 7 to complete the assembly. At the same time, squeeze and vent the gas in the annular airbag 24.

[0062] S2. Cut open the skin of the muscle to be sampled and place the muscle directly below the operating sleeve 2. Turn on the power of the air pump and insert the sampling mechanism 3 into the operating sleeve 2. Continuously press down on the sampling mechanism 3. The first annular cutter head 12 can cut the muscle. The muscle enters the outer sampling sleeve 6 and the second sampling sleeve along the first fixed sleeve 5. The muscle entering the outer sampling sleeve 6 is cut by the second annular cutter head 23. Some of the muscle will enter the inner sampling sleeve 22. Continue to press down on the sampling mechanism 3 until the first annular cutter head 12 reaches the bottom of the first annular slot 13. When the first annular cutter head 12 reaches the bottom of the first annular slot 13, the air inlet tube 26 is fully inserted into the air bag inlet tube 25. At the same time, the touch plate touches the sensor 27. The air pump works to inflate the annular air bag 24 (the gas pumped in one operation matches the overall volume of the annular air bag 24). Finally, pull out the sampling mechanism 3. The inflated annular air bag 24 can squeeze the muscle in the sampling mechanism 3 to prevent the muscle from falling out.

[0063] S3. Bring the sampling mechanism 3 after preliminary sampling to the laboratory. Place the sampling mechanism 3 horizontally on the table. Squeeze the two pressure plates 18 to bring the two guide sleeves 17 closer together, so that the two annular limiting inserts 14 are stored in the annular slots 13. Use a knife to cut along the gap between the outer sampling sleeve 6 and the first fixed sleeve 5 and the second fixed sleeve 7 to separate the muscle inside the outer sampling sleeve 6. Finally, take out the muscle inside the inner sampling sleeve 22 to complete the sampling.

[0064] The technical features of this invention not described can be implemented by or using existing technology, and will not be repeated here. Of course, the above description is not a limitation of this invention, and this invention is not limited to the examples above. Any changes, modifications, additions or substitutions made by those skilled in the art within the scope of this invention should also be within the protection scope of this invention.

Claims

1. An animal tissue sampling device, characterized in that, Includes a base and a sampling mechanism disposed on the base; An operating sleeve is provided on the base via a pair of inverted L-shaped support rods, and the sampling mechanism is movably disposed in the operating sleeve. The sampling mechanism includes a fixed frame movably inserted into the operating sleeve, an outer sampling sleeve is provided on the fixed frame, the fixed frame includes a U-shaped handle, a first fixed cylinder and a second fixed cylinder are respectively provided at both ends of the U-shaped handle, the outer sampling sleeve is provided between the first fixed cylinder and the second fixed cylinder, an annular groove is provided at both ends of the outer sampling sleeve, an annular limiting plate is provided in the annular groove on both sides, a first annular slot is provided on the opposite end face of the first fixed cylinder and the second fixed cylinder, and the annular limiting plate on both sides is respectively provided in the first annular slot on both sides; The first fixed cylinder has a first annular cutter head at the end away from the outer sampling sleeve, and the second fixed cylinder has an annular airbag at the inner side of the end near the outer sampling sleeve. An inner sampling sleeve is provided on the inner side of the outer sampling sleeve by several support rods. A second annular cutter head is provided on both ends of the inner sampling sleeve, and the ends of the second annular cutter head on both sides are flush with the ends of the outer sampling sleeve.

2. The animal tissue sampling device according to claim 1, characterized in that, A cross strut is provided on the inner side of the end of the second fixed cylinder away from the outer sampling sleeve, and an operating rod is provided on the cross strut.

3. The animal tissue sampling device according to claim 2, characterized in that, A pair of fixing plates are symmetrically arranged on the side walls at both ends of the outer sampling sleeve. The inner side of the fixing plates on both sides is provided with a slot that penetrates the interior of the annular sliding groove. A fixing seat is provided between the slots on both sides and on the side wall of the outer sampling sleeve. A guide rod is fixedly installed on the fixing seat. The two ends of the guide rod are respectively set on the fixing plates on both sides. A pair of annular limiting inserts are provided with guide sleeves near their ends. One end of the guide sleeve is provided on the annular limiting insert, and the other end of the guide sleeve extends out of the slot and is provided around the guide rod. A spring is provided between the guide sleeves on both sides and the fixed seat, and the spring is provided around the guide rod.

4. The animal tissue sampling device according to claim 3, characterized in that, The first fixed cylinder has a first limiting strip symmetrically arranged on both outer walls, and the second fixed cylinder has a pair of second limiting strips arranged on both outer walls corresponding to the positions of the first limiting strips; Pressure plates are provided on both sides of the guide sleeve; The inner side of the operating sleeve is provided with a vertically penetrating limiting groove corresponding to the positions of the first limiting strips on both sides, and the inner side wall of the operating sleeve is provided with corresponding receiving grooves corresponding to the positions of the pressure plate and the U-shaped handle.

5. The animal tissue sampling device according to claim 4, characterized in that, The base is provided with a second annular slot, which is concentrically arranged with the operating sleeve.

6. The animal tissue sampling device according to claim 5, characterized in that, Limit slots are formed on the outer periphery of the second annular slot and on the base corresponding to the positions of a pair of the first limiting strips; An airbag inlet pipe communicating with the inside of the annular airbag is provided in the first limiting strip on one side. The end of the airbag inlet pipe is set away from the outer sampling sleeve. The end of the airbag inlet pipe is flush with the end of the first limiting strip. A touch plate is provided at the end of the first limiting strip away from the outer sampling sleeve on the other side. The annular airbag is also provided with an airbag exhaust pipe, which passes through the first fixed cylinder; An air inlet tube is provided at the bottom of the limiting slot on one side, corresponding to the position of the airbag inlet tube. An air pump is also provided on the base. A sensor for use with the air pump is provided at the bottom of the limiting slot on the other side. The air inlet tube is connected to the air outlet of the air pump. Both the airbag inlet pipe and the airbag outlet pipe are equipped with one-way valves.

Images