Detection Method and System for Active Disinfection Equipment without Chemical Consumables Allowing Human-Machine Coexistence

Abstract

The present invention provides a detection method and a detection system for an active disinfection and sterilization device without chemical consumables that can coexist with humans. The detection method includes: Step A, placing the active disinfection and sterilization device in a test chamber, and after starting the device and running for a first period of time, performing: Step A1, measuring the concentration of by-products on the disinfection and sterilization factor output channel of the active disinfection and sterilization device; Step A2, collecting the disinfection and sterilization factors output by the active disinfection and sterilization device to obtain a liquid of disinfection and sterilization factors, and performing an acute inhalation toxicity test and / or a skin irritation test and / or an acute eye irritation test on the liquid of disinfection and sterilization factors. Testing in a clean and airtight test chamber can reduce the interference of the external environment and improve the test accuracy; this detection method not only detects the concentration of by-products, but also detects the harm of the disinfection and sterilization factors output by the active disinfection and sterilization device that directly acts on the human body, and can comprehensively and accurately evaluate the advantages and disadvantages of the human-machine coexistence of the active disinfection and sterilization device without chemical consumables, making the active disinfection and sterilization device safer and more reliable.

Description

Technical Field

[0001] The present invention relates to the field of detection of air disinfection and sterilization equipment, and particularly to a detection method and a detection system for an active disinfection and sterilization equipment without chemical consumables that can coexist with humans and machines. Background Art

[0002] At present, most of the air disinfection and purification equipment without chemical consumables applied in the market are passive disinfection. The air is circulated into the machine body, and various filters in the machine body are used to adsorb virus carriers, and then the air is blown out from the outlet. For example, HEPA disinfection equipment. A large number of germs are adsorbed on the filter screen of such disinfection and purification machines and are not disinfected. At the same time, there are problems such as bacteria breeding and secondary pollution in the machine body. For example, many hotels with central air-conditioning cannot be used as isolation places at present because there may be cross-infection among rooms caused by the central air-conditioning. Therefore, the air active disinfection and sterilization equipment without chemical consumables has ushered in a research and development and market boom.

[0003] The active disinfection and sterilization equipment without chemical consumables actively releases disinfection and sterilization factors into the indoor or outdoor environment. These disinfection and sterilization factors are preferably but not limited to ozone or ultraviolet rays or ions. Through the disinfection and sterilization factors, the germs and particulate matters in the air and on the object surfaces in the environment are disinfected and sterilized, so as to actively achieve safe, accurate and efficient disinfection and purification of the indoor environment that can coexist with humans and machines. At present, many active disinfection and sterilization equipment have emerged, such as ozone disinfection and sterilization equipment with appropriate output concentration, ultraviolet disinfection and sterilization equipment, and the air disinfection and sterilization device disclosed in the Chinese patent with the application number 202210014157.4, which discloses that hydrated plasma is used as the disinfection and sterilization factor.

[0004] In the prior art, before the active disinfection and sterilization equipment without chemical consumables is put on the market or during safety sampling inspection, only the concentration of the disinfection and sterilization factors output by it and whether the by-products meet the standards are detected. There are few detection indexes for the key point of the coexistence of humans and machines of the active disinfection and sterilization equipment without chemical consumables. It only considers the coexistence of humans and machines from the perspective of by-products, which is too simple, rough and one-sided, and cannot accurately evaluate the coexistence of humans and machines of the active disinfection and sterilization equipment without chemical consumables. In addition, the evaluation indexes for the disinfection and sterilization efficiency of the active disinfection and sterilization equipment without chemical consumables are also too simple. It is far from enough to only consider the concentration of the disinfection and sterilization factors output. Therefore, there is an urgent need to construct a complete set of detection schemes for the active disinfection and sterilization equipment without chemical consumables to objectively and accurately evaluate its coexistence of humans and machines and disinfection and sterilization efficiency. Summary of the Invention

[0005] The present invention aims to at least solve the technical problems existing in the prior art, and can objectively, accurately and comprehensively evaluate the disinfection and sterilization efficiency and the coexistence of humans and machines of the active disinfection and sterilization equipment. The present invention provides a detection method and a detection system for an active disinfection and sterilization equipment that can coexist with humans and machines.

[0006] To achieve the above object of the present invention, according to the first aspect of the present invention, a detection method for an active disinfection and sterilization device without chemical consumables that can coexist with humans and machines is provided, including: Step A, placing the active disinfection and sterilization device in a test chamber, and after starting the machine and running for the first period of time, performing: Step A1, measuring the by-product concentration on the disinfection and sterilization factor output channel of the active disinfection and sterilization device; Step A2, collecting the disinfection and sterilization factors output by the active disinfection and sterilization device to obtain a disinfection and sterilization factor liquid, and performing an acute inhalation toxicity experiment and / or a skin irritation experiment and / or an acute eye irritation experiment on the disinfection and sterilization factor liquid; the first period of time is from 10 minutes to 180 minutes.

[0007] The above technical solution: Testing in a clean and airtight test chamber can reduce the interference of the external environment and improve the test accuracy; this detection method not only detects the by-product concentration and judges the pros and cons of the human-machine coexistence of the active disinfection and sterilization device from the perspective of by-products, but also detects the harm of the disinfection and sterilization factors output by the active disinfection and sterilization device that directly acts on the human body, and can comprehensively and accurately evaluate the pros and cons of the human-machine coexistence of the active disinfection and sterilization device without chemical consumables, making the active disinfection and sterilization device that successfully passes this detection method safer and more reliable.

[0008] In a preferred embodiment of the present invention, in the said Step A, all or part of the four steps of Step A3, Step A4, Step A5, and Step A6 are also executed; Step A3, sampling the air in the test chamber and obtaining the number of particulate matters in the sampled air, denoted as the second particulate matter number, and obtaining the particulate matter cleanliness rate based on the second particulate matter number; Step A4, obtaining the germ disinfection rate of the object surface; Step A5, sampling the air in the test chamber and obtaining the natural bacteria content in the sampled air, denoted as the second natural bacteria content, and obtaining the natural bacteria sterilization rate based on the second natural bacteria content; Step A6, when the disinfection and sterilization factor output by the active disinfection and sterilization device is hydrated plasma, detecting the negative ion concentration in the disinfection and sterilization factor output by the active disinfection and sterilization device.

[0009] The above technical solution: After the active disinfection and sterilization device operates for the first time, one, two, three, or four of the steps from step A3 to step A6 can be executed. When two, three, or four steps are executed, testing can be carried out simultaneously, shortening the test cycle and improving the test efficiency. Step A3 obtains the cleanliness rate of the active disinfection and sterilization device for particulate matter in the air, and considers the disinfection and sterilization efficiency of the active disinfection and sterilization device from the perspective of particulate matter; step A4 can obtain the germ disinfection rate of the active disinfection and sterilization device for the surface of an object, and considers the disinfection and sterilization efficiency of the active disinfection and sterilization device from the aspect of surface sterilization rate; step A5 obtains the natural bacteria sterilization rate of the active disinfection and sterilization device, and considers the disinfection and sterilization efficiency of the active disinfection and sterilization device from the aspect of natural bacteria; step A6 detects the negative ion concentration. On the one hand, it can detect whether the active disinfection and sterilization device generates ion disinfection factors. On the other hand, since negative ions are beneficial to human health, detecting negative ions also detects whether the active disinfection and sterilization device pays attention to and is beneficial to human health, reflecting its friendliness in getting along with people. It can be seen that this detection method objectively and accurately detects the disinfection and sterilization efficiency and the coexistence of humans and machines of the active disinfection and sterilization device from multiple angles.

[0010] In a preferred embodiment of the present invention, in step A1, the ozone concentration is tested at a first distance from the disinfection factor outlet on the disinfection factor output channel of the active disinfection and sterilization device, and the TVOC concentration is tested at a second distance from the disinfection factor outlet on the disinfection factor output channel of the active disinfection and sterilization device. The first distance is 3 to 10 cm, and the second distance is 15 to 25 cm.

[0011] The above technical solution: realizes the simultaneous detection of two by-products at different locations, improving the test efficiency and test accuracy.

[0012] In a preferred embodiment of the present invention, step A4 specifically includes: setting a plurality of sampling points in the test chamber, arranging disinfection target objects at each sampling point, smearing the disinfection target objects with sterile cotton multiple times, testing the germ content at the smeared places of the sterile cotton, and comparing with the number of germs obtained by smearing the surface of the disinfection target object before disinfection to obtain the germ disinfection rate of the object surface.

[0013] The above technical solution: can test the germ disinfection rate of the object surface at multiple sampling points in the test chamber, and transform the detection of the number of germs on the object surface into the number of germs at the smeared places of the sterile cotton smeared on the disinfection target object, simplifying the test.

[0014] In a preferred embodiment of the present invention, it further includes step B, which specifically includes: placing the active disinfection and sterilization device in the test chamber, inputting aerosol containing microorganisms into the test chamber through an aerosol generator, and after starting and operating for the first time, detecting the microorganism disinfection rate, and the microorganism is Staphylococcus albus or Staphylococcus aureus or a virus.

[0015] The above technical solution: Detect the disinfection efficiency of the active disinfection device against viruses, Staphylococcus albus or Staphylococcus aureus, and detect the disinfection ability of the active disinfection device against foodborne bacteria, respiratory viruses and other pathogenic bacteria, reflecting the intensity of the active disinfection device's protection of human health and its friendliness in getting along with people.

[0016] To achieve the above object of the present invention, according to the second aspect of the present invention, a detection method for an active disinfection device without chemical consumables that can coexist with humans and machines is provided, including: Step A, place the active disinfection device in a test chamber, and after starting it up and running for the first time, perform at least two of the five steps of Step A1, Step A3, Step A4, Step A5, and Step A6; Step A1, measure the concentration of by-products on the disinfection factor output channel of the active disinfection device; Step A3, sample the air in the test chamber and obtain the number of particulate matters in the sampled air, denoted as the second particulate matter number, and obtain the particulate matter cleanliness rate based on the second particulate matter number; Step A4, obtain the disinfection rate of pathogenic bacteria on the surface of an object; Step A5, sample the air in the test chamber and obtain the natural bacteria content in the sampled air, denoted as the second natural bacteria content, and obtain the natural bacteria disinfection rate based on the second natural bacteria content; Step A6, when the disinfection factor output by the active disinfection device is hydrated plasma, detect the negative ion concentration in the disinfection factor output by the active disinfection device; the first time is from 10 minutes to 180 minutes.

[0017] The above technical solution: Testing in a clean and sealed test chamber can reduce the interference of the external environment and improve the test accuracy; after the active disinfection device runs for the first time, perform at least two of the five steps of Step A1, Step A3, Step A4, Step A5, and Step A6, which can be carried out simultaneously, shortening the test cycle and improving the test efficiency. Through Step A1, the by-product situation output by the active disinfection device can be obtained, and the quality of the human-machine coexistence of the active disinfection device can be judged from the perspective of by-products; Step A3 obtains the particulate matter cleanliness rate of the active disinfection device for the air in the test chamber, and the disinfection efficiency of the active disinfection device is considered from the perspective of particulate matter; Step A4 can obtain the disinfection rate of pathogenic bacteria on the surface of an object by the active disinfection device, and the disinfection efficiency of the active disinfection device is considered from the aspect of surface disinfection rate; Step A5 obtains the natural bacteria disinfection rate of the active disinfection device, and the disinfection efficiency of the active disinfection device is considered from the aspect of natural bacteria; Step A6 detects the negative ion concentration. On the one hand, it can detect whether the active disinfection device generates an ionic disinfection factor. On the other hand, since negative ions are beneficial to human health, detecting negative ions also detects whether the active disinfection device pays attention to and is beneficial to human health, reflecting its friendliness in getting along with people. It can be seen that this detection method objectively and accurately detects the disinfection efficiency and human-machine coexistence of the active disinfection device from multiple angles.

[0018] To achieve the above object of the present invention, according to the third aspect of the present invention, the present invention provides a detection system for a detection method of an active disinfection and sterilization device without chemical consumables capable of coexisting with humans and machines according to the first aspect of the present invention, including a test chamber, and an air sampler, an ozone analyzer, a gas chromatograph, and a particle counter disposed in the test chamber; the output end of the air sampler is respectively connected to the input end of the ozone analyzer, the input end of the gas chromatograph, and the input end of the particle counter.

[0019] In a preferred embodiment of the present invention, it further includes: a negative ion tester disposed on the disinfection and sterilization factor output channel of the active disinfection and sterilization device for monitoring the negative ion concentration; an aerosol generator for outputting aerosol containing microorganisms to the test chamber; an impact sampler for sampling the air in the test chamber.

[0020] The above technical solution: This detection system can objectively and accurately detect the disinfection and sterilization efficiency and the coexistence of humans and machines of the active disinfection and sterilization device without chemical consumables from multiple angles. Specific Embodiments

[0021] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0022] In the description of the present invention, unless otherwise specified and limited, it should be noted that the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a mechanical connection or an electrical connection, or it can be the communication inside two elements. It can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms can be understood according to specific circumstances.

[0023] Embodiment 1

[0024] This embodiment discloses a detection method for an active disinfection and sterilization device without chemical consumables capable of coexisting with humans and machines, including: Step A, placing the active disinfection and sterilization device in the test chamber, and after starting it up and running for the first time, performing: Step A1, measuring the by-product concentration on the disinfection and sterilization factor output channel of the active disinfection and sterilization device; Step A2, collecting the disinfection and sterilization factors output by the active disinfection and sterilization device to obtain a disinfection and sterilization factor liquid, and performing an acute inhalation toxicity experiment and / or a skin irritation experiment and / or an acute eye irritation experiment on the disinfection and sterilization factor liquid; the first time is from 10 minutes to 180 minutes.

[0025] In this embodiment, in step A1, the by-product concentration is measured on the disinfection factor output channel of the active disinfection device. Specifically, it includes: measuring the ozone concentration at a first distance from the disinfection factor outlet on the disinfection factor output channel of the active disinfection device, and measuring the TVOC concentration at a second distance from the disinfection factor outlet on the disinfection factor output channel of the active disinfection device. The first distance is 3 to 10 cm, preferably 5 cm, and the second distance is 15 to 25 cm, preferably 20 cm. An air sampler is used to collect the air at the first distance and the second distance from the disinfection factor outlet respectively, and the collected air at the two places is input into an ozone analyzer and a gas chromatograph respectively, which are used to monitor the ozone concentration and the TVOC concentration respectively. TVOC is the English abbreviation of "Total Volatile Organic Compounds", which means total volatile organic compounds. The by-products detected here are mainly ozone and TVOC, and other by-products such as nitrides can also be tested. Further preferably, before starting up, the ozone concentration at the first distance is pre-tested as the ozone background concentration, and the TVOC concentration at the second distance is pre-tested as the TVOC background concentration. The ozone concentration generated by the active disinfection device is obtained by subtracting the ozone background concentration from the ozone concentration measured at the first time after starting up, and the TVOC concentration generated by the active disinfection device is obtained by subtracting the TVOC background concentration from the TVOC concentration measured at the first time after starting up. The detailed steps of ozone detection can be referred to the ultraviolet photometric method in the determination of ambient air ozone in standard HJ590-2010, which will not be elaborated here. The detailed steps of TVOC detection can be referred to the steps provided in Appendix C of the indoor air quality standard GB / T 18883-2002, which will not be elaborated here.

[0026] In this embodiment, step A2 is to collect the disinfection factors output by the active disinfection device to obtain a disinfection factor liquid, and perform an acute inhalation toxicity experiment and / or a skin irritation experiment and / or an acute eye irritation experiment on the disinfection factor liquid. An absorption hood can be used to collect the disinfection factor liquid on the disinfection factor output channel. Since the disinfection factor generally contains water vapor, the disinfection factor liquid can be collected.

[0027] In this embodiment, the acute inhalation toxicity experiment, the skin irritation experiment, and the acute eye irritation experiment can all be carried out according to the steps in the "Disinfection Technical Specification" (2002 edition).

[0028] In an application scenario of this embodiment, the process of the acute inhalation toxicity experiment is as follows:

[0029] 1. Equipment and animals

[0030] Test sample: The disinfection factor liquid of the active disinfection device (highest application concentration: stock solution).

[0031] Animals: 20 KM mice, 10 males and 10 females, with body weights ranging from 18 g to 22 g, provided by Spiford (Beijing) Biotechnology Co., Ltd., SPF-class animals, production license number SCXK (Jing) 2019-0010, quality certificate number 110324210104034474. The feed was provided by Spiford (Beijing) Biotechnology Co., Ltd., production license number SCXK (Jing) 2019-0010, quality certificate number 1103242100046455. The temperature in the animal room was 19°C - 26°C, and the relative humidity was 40% - 70%. There was 12 h of lighting and 12 h of darkness. The animals had free access to food and water.

[0032] Reagents and instruments: Electronic balance (model: ME4002E / 02, serial number: DL030), electronic balance (model: ME403E / 02, serial number: DL031), inhalation exposure cabinet (model: 300L, serial number: DL040).

[0033] 2. Methods

[0034] Detection basis: Item 2.3.2 of the "Disinfection Technical Specification" (2002 edition).

[0035] Dose grouping: Based on the results of the preliminary acute inhalation toxicity test of the test sample on mice. In this test, a single dose group of 10000 mg / m³ was set, with 20 KM mice, 10 males and 10 females.

[0036] Preparation of the test sample: The original solution of the test sample was used.

[0037] Exposure method: First, place 20 mice in the inhalation exposure cabinet (volume 0.3 cubic meters). Then, take 3.004 g of the exposure liquid and add it to the nebulizer, and inject it into the exposure cabinet within 20 min for a single 2-h exposure.

[0038] Observation: After exposure, observe and record the poisoning manifestations of the animals. At the end of the observation period, sacrifice the animals for autopsy and macroscopic observation. If any abnormal tissues or organs are found, further histopathological examinations are performed. The observation period is 14 days.

[0039] Evaluation index: According to the "Disinfection Technical Specification" (2002 edition), for a single 2-h inhalation exposure concentration of 10000 mg / m³, if there are no deaths within 14 days, it can be determined that the LC50 (the toxic dose that causes half of the test animals to die, called the median lethal dose) is greater than 10000 mg / m³.

[0040] In an application scenario of this embodiment, the process of the skin irritation experiment is as follows:

[0041] I. Equipment and animals

[0042] Test sample: The disinfection factor liquid of the active disinfection equipment (highest application concentration: stock solution).

[0043] Animals: 3 Japanese white rabbits with big ears, female, weighing 2.0 kg - 2.5 kg, provided by Beijing Changyang Xishan Farm. They are ordinary-grade animals, with the production license number SCXK (Jing) 2016 - 0007 and the quality certificate number 110329211100078146. The feed is provided by Spif (Beijing) Biotechnology Co., Ltd., with the production license number SCXK (Jing) 2019 - 0010 and the quality certificate number 1103242100044961. The temperature in the animal house is 19°C - 26°C, and the relative humidity is 40% - 70%. There is 12-hour lighting and 12-hour darkness. The animals are allowed to feed freely and drink water freely.

[0044] II. Methods

[0045] Detection basis: Item 2.3.3.3.3 of "Disinfection Technical Specification" (2002 edition).

[0046] Preparation of test sample: Use the stock solution of the test sample.

[0047] Exposure method: 24 hours before the test, depilate the skin on both sides of the spine on the back of the Japanese white rabbits with big ears, with an area of about 3 cm * 3 cm. The next day, apply 0.5 mL of the stock solution of the test sample on the left skin with an area of 2.5 cm * 2.5 cm, and use the right side as a blank control. After 4 hours of application, wash it with warm water to remove the residual test sample. Apply it once a day in the same manner as described above for 14 consecutive days. In order to facilitate the application of the test sample and the observation of the results, depilate again if necessary.

[0048] Observation and evaluation: Observe the skin reaction and score it 24 hours after each application. The scoring criteria and the classification of irritation intensity refer to Tables 2 - 11 and 2 - 12 in "Skin Irritation Experiment" of "Disinfection Technical Specification" (2002 edition).

[0049] In another application scenario of this embodiment, the process of the acute eye irritation experiment is as follows:

[0050] 1. Equipment and animals

[0051] Test sample: The disinfection factor liquid of the active disinfection equipment (highest application concentration: stock solution).

[0052] Animals: 3 Japanese white rabbits with big ears, female, weighing 2.0 kg - 2.5 kg, provided by Beijing Changyang Xishan Breeding Farm, ordinary-grade animals, production license number SCXK (Beijing) 2016-0007, quality certificate number 110329211100078146. The feed was provided by Spbio (Beijing) Biotechnology Co., Ltd., production license number SCXK (Beijing) 2019-0010, quality certificate number 1103242100044961. The temperature in the animal house was 19°C - 26°C, and the relative humidity was 40% - 70%. There was 12-hour lighting and 12-hour darkness. The animals were allowed to eat freely and drink water freely.

[0053] 2. Methods

[0054] Detection basis: Item 2.3.4 of "Disinfection Technical Specification" (2002 edition).

[0055] Preparation of the test sample: Use the undiluted test sample.

[0056] Exposure method: Draw 0.1 mL of the undiluted test sample and drop it into the left conjunctival sac of the Japanese white rabbit with big ears. After dropping the test sample, passively close the eye for 4 s, and rinse with normal saline 30 s later. Drop normal saline into the right eye as a normal control.

[0057] Observation and evaluation: At 1 h, 24 h, 48 h, 72 h, 7 d, 14 d, and 21 d after eye drops, observe the damage and recovery of the conjunctiva, iris, and cornea of the Japanese white rabbit with big ears with the naked eye. If no irritation reaction occurs within 72 h, or the eye irritation reaction completely recovers on the 7th day or the 14th day, terminate the test in advance. The scoring criteria and irritation intensity classification refer to Tables 2-13 and 2-14 in "Acute Eye Irritation Test" of Item 2.3.4 of "Disinfection Technical Specification" (2002 edition).

[0058] In this embodiment, preferably, in step A, it further includes performing all or part of the four steps of step A3, step A4, step A5, and step A6; step A3, sampling the air in the test chamber and obtaining the number of particulate matters in the sampled air, denoted as the second particulate matter number, and obtaining the particulate matter cleanliness rate based on the second particulate matter number; step A4, obtaining the germ killing rate of the object surface; step A5, sampling the air in the test chamber and obtaining the natural bacteria content in the sampled air, denoted as the second natural bacteria content, and obtaining the natural bacteria elimination rate based on the second natural bacteria content; step A6, when the disinfection factor output by the active disinfection device is hydrated plasma, detecting the negative ion concentration in the disinfection factor output by the active disinfection device.

[0059] In this embodiment, the four steps of step A3, step A4, step A5, and step A6 can be executed simultaneously or at different times. When the combined steps are executed at different times, each step can correspond to a different first time. When the four steps are executed simultaneously, the internal temperature of the test chamber can be set to 25°C and the humidity can be set to 60%RH. The following is an introduction to each step:

[0060] Step A3: Sample the air in the test chamber and obtain the number of particles in the sampled air, denoted as the second particle number. Obtain the particle cleanliness rate based on the second particle number. Before starting the machine, first collect the same volume of air from the test chamber and obtain the number of particles in this air, denoted as the first particle number. Obtain the particle cleanliness rate by dividing the difference between the second particle number and the first particle number by the first particle number. The diameter of the particles is greater than or equal to 0.3 microns. The number of particles can be obtained by a high-concentration particle counter SX-L301N. It is also possible to refer to the index of clean air volume in Appendix B of GB / T 18801-2015 Air Purifier to judge the disinfection efficiency of the active disinfection device for particles. The clean air volume Q (m³ / h) = 60*(Ke*Kn)*V (Ke is the total attenuation constant, Kn is the natural attenuation constant, and V is the volume of the test chamber). The specific process is not elaborated here.

[0061] Step A4: Obtain the disinfection rate of germs on the surface of the object. Step A4 specifically includes: Set multiple sampling points in the test chamber, preferably but not limited to 15. Arrange disinfection target objects at each sampling point. The disinfection target objects are preferably but not limited to fabrics, plastics, or wooden boards. Use a sterile cotton swab to smear the disinfection target object multiple times, such as 8 times horizontally and vertically back and forth. The sterile cotton can be a sterile cotton swab. Test the germ content at the smeared area of the sterile cotton and compare it with the number of germs obtained by smearing the surface of the disinfection target object before disinfection to obtain the disinfection rate of germs on the surface of the object. Preferably but not limited to, cut the smeared area of the sterile cotton into a dilution solution, then shake it 80 times, put it into a culture medium, and obtain the germ content in the culture medium as the germ content of the disinfection target object after disinfection. Before disinfection, another sterile cotton swab can be repeatedly smeared on another part of the same disinfection target object (i.e., the part of the disinfection target object other than the smeared area of the sterile cotton after disinfection) or on another target object with the same germ content as the disinfection target object, cut the smeared area into a dilution solution, then shake it 80 times, put it into a culture medium, and obtain the germ content in the culture medium as the germ content of the disinfection target object before disinfection. Another target object is placed at the same sampling point as the disinfection target object. The dilution solution is preferably but not limited to a PBS solution containing 0.1% Tween 80 (i.e., phosphate buffer solution, generally prepared with Na₂HPO₄ and KH₂PO₄). The detection basis can refer to the "Disinfection Technical Specification" (2002 edition), which is not elaborated here.

[0062] Step A5: Sample the air in the test chamber and obtain the natural bacteria content in the sampled air, denoted as the second natural bacteria content. Based on the second natural bacteria content, obtain the natural bacteria sterilization rate. Specifically, test the natural bacteria content in the test chamber before starting the machine, denoted as the first natural bacteria content. The air in the test chamber can be sampled by an impact sampler, such as a six-stage sieve air impact sampler. The specific test method for obtaining the natural bacteria content based on the sampled air can refer to the technical solution disclosed in the special requirements for air purifiers with antibacterial, sterilization, and purification functions for household and similar electrical appliances in Standard GB21551.3-2010, which will not be elaborated here. The natural bacteria sterilization rate K11 (%) = (V01 - V11) / V01 (where V01 is the first natural bacteria content and V11 is the second natural bacteria content).

[0063] Step A6: When the disinfection factor output by the active disinfection device is hydrated plasma, detect the negative ion concentration in the disinfection factor output by the active disinfection device. The negative ion concentration can be tested using a negative ion tester on the disinfection factor output channel.

[0064] In this embodiment, preferably, it further includes Step B, specifically including: Place the active disinfection device in the test chamber, input an aerosol containing microorganisms into the test chamber through an aerosol generator, and after running for the first period of time, detect the microorganism sterilization rate. The microorganisms are Staphylococcus albus or Staphylococcus aureus or viruses, and the virus is preferably but not limited to the novel coronavirus.

[0065] In this embodiment, Step B and Step A cannot be carried out simultaneously and can be carried out successively. In an application scenario of this embodiment, the specific test process of Step B is as follows:

[0066] I. Detection of Staphylococcus albus and Staphylococcus aureus:

[0067] Detection method: Refer to Appendix A of the Special Requirements for Air Purifiers with Antibacterial, Sterilization, and Purification Functions for Household and Similar Electrical Appliances in GB21551.3-2010.

[0068] Test strains: Staphylococcus albus 8032, Staphylococcus aureus ATCC6538.

[0069] Test conditions: Ambient temperature: (20 - 25)°C, ambient humidity: (50 - 70)%RH.

[0070] Test equipment: Test chamber (30 cubic meters), six-stage sieve hole air impact sampler (FA-1), microorganism aerosol generator, nutrient agar medium.

[0071] Machine operating state: Turn on the "highest wind speed gear" during the test process.

[0072] Calculation formula: Natural sales rate N12 (%) = (V02 - V12) / V02 * 100 (V02 is the airborne bacteria content before the test in the control group, V12 is the airborne bacteria content after the test in the control group);

[0073] Bacteria removal rate K12 (%) = ((V12 * (1 - N12) - V22) / (V12 * (1 - N12)) * 100 (V12 is the airborne bacteria content before the test in the experimental group, V22 is the airborne bacteria content after the test in the experimental group).

[0074] II. Coronavirus detection:

[0075] Detection method: Refer to the "Disinfection Technical Specification" 2002 Edition, 2.1.3 Air Disinfection Identification Test.

[0076] Test objects: Strains: Coronavirus HCoV-229E (VR-740), Cells: Huh-7 cells.

[0077] Test equipment: 2 test chambers (30 cubic meters) (one as the control group, one as the experimental group), sampling pump, aerosol generator, liquid impinger sampler.

[0078] Test conditions: Ambient temperature: 20 - 25°C; Ambient humidity: 50 - 70% RH.

[0079] Machine operating status: "Highest wind speed gear" is turned on during the test process.

[0080] Test steps:

[0081] Adjust the temperature and relative humidity of the test chamber to the test requirements;

[0082] Put all the used equipment into the test chamber at one time and close the chamber door;

[0083] Turn on the aerosol generator to atomize the virus, and at the same time use a fan to stir. After atomizing the virus, let it stand for a certain period of time;

[0084] Sample before purification for the control group and the experimental group respectively;

[0085] Carry out purification in the experimental group test chamber, and the control group test chamber is used as a control;

[0086] After acting for the specified time, sample the experimental group and the control group simultaneously;

[0087] Detect the virus titer of the recovered samples and proceed as follows:

[0088] a) Dilute the above recovered solution by 10-fold serial dilution;

[0089] b) Add the dilution solution to a 96-well cell culture plate containing MDCK cells grown to a monolayer, and set up a normal control group, adding an equal amount of culture medium.

[0090] c) Incubate in an incubator at 37 °C and 5% CO2 for 60 min, then discard the supernatant, add maintenance culture medium containing 400 IU / mL of double antibodies, and continue to incubate for 3 - 5 days, observing the cell growth status every day.

[0091] d) When the MDCK cells inoculated with the virus show pathological changes such as rounding or shrinking, record the occurrence of cytopathic effects.

[0092] e) Calculate the median infective dose TCID50 according to the Reed-Muench formula.

[0093] Calculate the virus titer and killing rate in the sample, and repeat the experiment three times.

[0094] Calculation formula:

[0095] Natural attenuation rate N13 (%) = (V03 - V13) / V03 (V03 is the air virus titer before the experiment in the control group, V13 is the air virus titer after the experiment in the control group);

[0096] Clearance rate K13 (%) = (V13 * (1 - N13) - V23) / (V13 * (1 - N13)) (V13 is the air virus titer before the experiment in the experimental group, V23 is the air virus titer after the experiment in the experimental group).

[0097] Example 2

[0098] This example discloses a detection method for an active disinfection and sterilization device that can coexist with humans and has no chemical consumables, including: Step A, place the active disinfection and sterilization device in the test chamber, and after starting it up and running for the first time, perform at least two of the five steps of Step A1, Step A3, Step A4, Step A5, and Step A6. The selected steps can be executed simultaneously or at different times; Step A1, measure the by-product concentration on the disinfection factor output channel of the active disinfection and sterilization device; Step A3, sample the air in the test chamber and obtain the number of particulate matters in the sampled air, denoted as the second particulate matter number, and obtain the particulate matter cleanliness rate based on the second particulate matter number; Step A4, obtain the germ killing rate on the object surface; Step A5, sample the air in the test chamber and obtain the natural bacteria content in the sampled air, denoted as the second natural bacteria content, and obtain the natural bacteria sterilization rate based on the second natural bacteria content; Step A6, when the disinfection factor output by the active disinfection and sterilization device is hydrated plasma, detect the negative ion concentration in the disinfection factor output by the active disinfection and sterilization device; The first time is from 10 minutes to 180 minutes.

[0099] In this embodiment, preferably, step A further includes step A2: Step A2 is to collect the disinfection factors output by the active disinfection device to obtain a disinfection factor liquid, and perform an acute inhalation toxicity test and / or a skin irritation test and / or an acute eye irritation test on the disinfection factor liquid.

[0100] In this embodiment, preferably, it further includes step B, which specifically includes: placing the active disinfection device in the test chamber, inputting an aerosol containing microorganisms into the test chamber through an aerosol generator, and after starting the machine and running for the first period of time, detecting the microorganism disinfection rate, where the microorganisms are Staphylococcus albus or Staphylococcus aureus or viruses.

[0101] In this embodiment, preferably, step A4 specifically includes: setting multiple sampling points in the test chamber, arranging disinfection target objects at each sampling point, smearing the disinfection target objects multiple times with a sterile cotton swab, testing the pathogen content at the smeared part of the sterile cotton swab, and comparing it with the number of pathogens obtained by smearing the surface of the disinfection target object before disinfection to obtain the pathogen disinfection rate on the object surface.

[0102] In this embodiment, the specific steps of steps A1 to A6 can be referred to the detailed description in Embodiment 1, and will not be elaborated herein.

[0103] In an application scenario of this embodiment, steps A1 to A6 are independently tested respectively, and the specific process includes:

[0104] Step A1, that is, the release amount of harmful substances: detection of ozone concentration (5 cm from the air outlet) and TVOC concentration (20 cm from the air outlet), detection description:

[0105] Detection method: Ozone: Ultraviolet photometric method for the determination of ozone in ambient air HJ590 - 2010; TVOC concentration: Appendix C of GB / T18883 - 2002 Indoor air quality standard.

[0106] Test equipment: Ozone analyzer (106 - L), air sampler (2020), gas chromatograph (GC - 2010Pro).

[0107] Machine operating state: During the test process, turn on the "highest wind speed gear", and the machine is preheated for 20 minutes before testing.

[0108] Test steps: Place the device to be tested in the test chamber. Measure the ambient background concentration value. Turn on the machine and test the concentration values of each index according to the standard requirements.

[0109] Step A5, detection of natural air bacteria:

[0110] Detection method: Appendix A of GB21551.3 - 2010 Special requirements for antibacterial, sterilizing and purifying functions of household and similar electrical appliances - Air purifiers.

[0111] Detection conditions: Ambient temperature: (27 - 29) °C; Ambient humidity: (70 - 73) %RH.

[0112] Test site: Test chamber.

[0113] Culture medium: Ordinary nutrient agar medium

[0114] Sampler: Six - stage sieve - hole air impact sampler.

[0115] Machine operation status: "Highest wind speed gear" is turned on during the test process.

[0116] Calculation formula:

[0117] Bactericidal rate K11 (%) = (V01 - V11) / V01 (where V01 is the airborne bacteria content before the test and V11 is the airborne bacteria content after the test).

[0118] Step A3, Detection of clean air volume of particulate matter:

[0119] Detection method: Appendix B of GB / T18801 - 2015 Air purifier.

[0120] Test object: Total number of particulate matters above 0.3μm.

[0121] Test conditions: Ambient temperature: (25 ± 2) °C; Ambient humidity: (50 ± 10) %RH.

[0122] Test equipment: Test chamber (30 cubic meters), High - concentration particle counter (SX - L301N).

[0123] Machine operation status: "Highest wind speed gear" is turned on during the test process; The active disinfection equipment is pre - heated for 20 min before testing.

[0124] Calculation formula:

[0125] Clean air volume Q (m³ / h) = 60*(Ke*Kn)*V (where Ke is the total attenuation constant, Kn is the natural attenuation constant, and V is the volume of the test chamber)

[0126] Step A4, On - site disinfection test (object surface)

[0127] Diluent: PBS solution containing 0.1% Tween 80.

[0128] Disinfection object: Fabric.

[0129] Equipment: Sterile cotton swabs, timer, graduated pipettes (1.0 mL, 1.5 mL, 10.0 mL), etc.

[0130] Test site: Test chamber.

[0131] Method: Detection basis: Refer to the "Disinfection Technical Specification" (2002 version);

[0132] Before disinfection: Distribute 15 sampling points in a closed space. Set two control groups and two test groups at each sampling point. Moisten a sterile cotton swab in a test tube containing 5 mL of dilution solution, smear and sample a block, making 8 horizontal and vertical back-and-forth strokes each. After sampling, cut the sampled end of the cotton swab into the original dilution solution test tube in a sterile operation manner, shake it 80 times, and appropriately dilute it to obtain a positive control group sample.

[0133] In a test chamber with a volume of approximately 30 cubic meters, hang the fabric at different positions in the space. Place the sample in the center of the space, and turn on the sample machine at "highest gear" for disinfection for 120 minutes. After disinfection, smear and sample the disinfected block in the same way as the positive control group to obtain a test group sample. The same batch of dilution solution and culture medium are used as negative control group samples.

[0134] Number of subjects: 30 blocks, the test is repeated 3 times, the test environmental temperature is 22°C - 25°C, and the relative humidity is 63% - 67%.

[0135] Step A6, Negative ion concentration detection:

[0136] Detection method: Refer to Appendix C of QB / T 4982-2016 Negative Ion Generators for Household and Similar Appliances.

[0137] Test equipment: Negative ion tester

[0138] Test conditions: Ambient temperature: (23 ± 2)°C; Ambient humidity: (50 ± 10)%RH.

[0139] Example 3

[0140] This example discloses a detection system for the detection method of an active disinfection and sterilization device without chemical consumables that can coexist with humans based on the above, including a test chamber, and an air sampler, an ozone analyzer, a gas chromatograph, and a particle counter provided in the test chamber; the output end of the air sampler is respectively connected to the input end of the ozone analyzer, the input end of the gas chromatograph, and the input end of the particle counter. The size of the test chamber is 30 cubic meters. The air sampler can be a common indoor air sampler, such as TQC-1500Z. The model of the ozone analyzer can be 106-L, and the model of the gas chromatograph can be GC-2010Pro.

[0141] In this embodiment, preferably, it further includes a negative ion tester disposed on the disinfection factor output channel of the active disinfection device for monitoring the negative ion concentration; an aerosol generator for outputting aerosol containing microorganisms to the test chamber; an impact sampler for sampling the air in the test chamber, preferably but not limited to a six-stage sieve-hole air impact sampler or a liquid impact sampler.

[0142] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0143] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A detection method for an active disinfection device without chemical consumables that can coexist with humans and machines, characterized in that, Including: Step A: Place the active disinfection and sterilization device in the test chamber. After starting the device and running for the first period of time, perform the following: Step A1: Measure the concentration of by-products on the disinfection and sterilization factor output channel of the active disinfection and sterilization device. The by-products are ozone and TVOC, and the disinfection and sterilization factor contains water vapor. Step A2: Use an absorption hood to collect the disinfection and sterilization factors output by the active disinfection and sterilization device on the disinfection and sterilization factor output channel to obtain a liquid of disinfection and sterilization factors, and conduct an acute inhalation toxicity test and / or a skin irritation test and / or an acute eye irritation test on the liquid of disinfection and sterilization factors. The first period of time is from 10 minutes to 180 minutes. In Step A1, measure the ozone concentration at a first distance from the outlet of the disinfection and sterilization factor on the disinfection and sterilization factor output channel of the active disinfection and sterilization device, and measure the TVOC concentration at a second distance from the outlet of the disinfection and sterilization factor on the disinfection and sterilization factor output channel of the active disinfection and sterilization device. The first distance is from 3 to 10 cm, and the second distance is from 15 to 25 cm. Before starting the device, pre-measure the ozone concentration at the first distance as the ozone background concentration, and pre-measure the TVOC concentration at the second distance as the TVOC background concentration. Subtract the ozone background concentration from the ozone concentration measured after the first period of time of starting the device to obtain the ozone concentration generated by the active disinfection and sterilization device, and subtract the TVOC background concentration from the TVOC concentration measured after the first period of time of starting the device to obtain the TVOC concentration generated by the active disinfection and sterilization device. Step A6: The disinfection and sterilization factor output by the active disinfection and sterilization device is hydrated plasma. Detect the negative ion concentration in the disinfection and sterilization factor output by the active disinfection and sterilization device.

2. The detection method of the active disinfection device without chemical consumables that can coexist with humans and machines according to claim 1, characterized in that, In Step A, it also includes performing all or part of the three steps of Step A3, Step A4, and Step A5. Step A3: Sample the air in the test chamber and obtain the number of particulate matters in the sampled air, denoted as the second number of particulate matters, and obtain the particulate matter cleanliness rate based on the second number of particulate matters. Step A4: Obtain the germ disinfection rate on the surface of an object. Step A5: Sample the air in the test chamber and obtain the natural bacteria content in the sampled air, denoted as the second natural bacteria content, and obtain the natural bacteria sterilization rate based on the second natural bacteria content.

3. The detection method of the active disinfection device without chemical consumables capable of coexisting with humans and machines according to claim 2, characterized in that, Step A4 specifically includes: Set multiple sampling points in the test chamber, arrange disinfection target objects at each sampling point, smear the disinfection target objects with sterile cotton multiple times, test the germ content at the smeared parts of the sterile cotton, and compare it with the number of germs obtained by smearing the surface of the disinfection target object before disinfection to obtain the germ disinfection rate on the surface of the object.

4. The detection method of the active disinfection device without chemical consumables that can coexist with humans and machines according to claim 1 or 2 or 3, characterized in that, It also includes Step B, which specifically includes: Place the active disinfection and sterilization device in the test chamber, input an aerosol containing microorganisms into the test chamber through an aerosol generator, and after starting the device and running for the first period of time, detect the microorganism disinfection rate. The microorganism is Staphylococcus albus or Staphylococcus aureus or a virus.

5. A detection system for the detection method of an active disinfection device without chemical consumables that can coexist with humans and machines according to one of claims 1-4, characterized in that, Including a test chamber, and an air sampler, an ozone analyzer, a gas chromatograph, and a particle counter provided in the test chamber. The output end of the air sampler is respectively connected to the input end of the ozone analyzer, the input end of the gas chromatograph, and the input end of the particle counter.

6. The detection system according to claim 5, wherein, It also includes: A negative ion tester provided on the disinfection and sterilization factor output channel of the active disinfection and sterilization device for monitoring the negative ion concentration. An aerosol generator for outputting an aerosol containing microorganisms to a test chamber; An impact sampler for sampling the air in the test chamber.