SNP loci for identification of apricot varieties and their applications

Abstract

The present invention relates to SNP sites and applications thereof for identifying apricot varieties. The present invention establishes a set of scientific and standardized identification methods for apricot germplasm resources based on SNP technology, which can facilitate the efficient classification and management of apricot plants. The method has good specificity and can serve as a key credential for determining apricot species, filling the technical gap in the development of apricot SNP molecular markers in the prior art. The present invention provides 29 SNP molecular markers suitable for apricot plants, which can achieve high-throughput operation, have extremely high accuracy, and simple data reading, can save costs and improve efficiency. The 29 SNP specific sites provided by the present invention are of great significance for the scientific preservation, rapid identification, breeding, classification and management of apricot fruit and vegetable resources, and play an extremely important role in the preservation, breeding and genetic diversity research of apricot fruit tree resources.

Description

Technical Field

[0001] The present invention relates to the field of plant variety identification, and specifically relates to SNP sites for apricot variety identification and applications thereof. Background Art

[0002] Apricot varieties are diverse, and their unique growing environment and long-term evolution have resulted in a rich and valuable germplasm resource. From a resource perspective, there are 10 species worldwide, 9 of which are native to China, with 13 additional variants. Nearly 500 hybrid varieties exist in Xinjiang, my country. Because most apricot varieties in Xinjiang have been grown naturally and extensively for many years without improvement, the nutritional content of different apricot varieties varies greatly. The almond processing industry is low in concentration, resulting in low added value. Furthermore, the vast majority of existing varieties are unsuitable for processing or have not been fully utilized and developed, resulting in significant losses of apricot functional components and severe environmental pollution. These problems have become bottlenecks restricting the development of Xinjiang's apricot industry, severely hindering the protection, utilization, and germplasm innovation of apricot plant resources. Therefore, fully exploring the nutritional value of different apricot varieties is an urgent issue that needs to be addressed, hoping to provide a scientific basis for the selection of improved varieties, species diversity, and sustainable development of my country's apricot industry.

[0003] With the continuous acceleration of apricot variety breeding in my country, the number of cultivated apricot varieties is increasing. Therefore, the effective and accurate classification and identification of different cultivated apricot varieties is an urgent problem to be solved. Currently, the identification of cultivated apricot varieties in Xinjiang is still limited to traditional methods such as tree morphology, flower form, fruit appearance, and fruit maturity, which are greatly affected by environmental and human factors.

[0004] Most cultivated apricots worldwide originate in China and were introduced abroad via the Silk Road over 2,000 years ago. Apricot cultivation in my country is extensive and rich in varieties. Xinjiang is the province with the largest apricot planting area in the country. Over 90% of Xinjiang's apricots are grown in southern Xinjiang oasis areas such as Kashgar and Kuqa. Apricot production accounts for approximately 50% of the country's total fresh apricot production, and the apricot industry has become a key pillar of the local economy. The main cultivated varieties are similar to those found in Central Asian countries, belonging to the ancient Central Asian ecological group, and differ significantly from apricot varieties found in other parts of my country. Within this Central Asian ecological group, a variety of varieties are found, with strong cold resistance, vigorous growth, numerous slender branches, and small leaves. They have a long winter dormancy period, a late flowering period, and strong overwintering ability. They bloom profusely but are not self-fertile. They are high-yielding, with small, often oblong, soft fruits that are high in sugar and low in acid. The kernels are large, and the fruits are often used for drying.

[0005] Single nucleotide polymorphisms (SNPs) refer to variations in a single nucleotide in the genome, including substitutions, transversions, deletions, and insertions. By detecting differences in single nucleotides at the molecular level, SNP markers can help distinguish differences in the genetic material of two individuals. SNPs are considered the third generation of DNA molecular marker technology and are expected to become the most important and effective molecular marker. However, because the total number of SNP markers in various cultivated apricot varieties reaches over 19 million, the application of SNP technology alone in apricot germplasm identification and genetic relationship research is largely unsuccessful, and there are currently no reports on the development of SNP molecular markers specifically for apricot. Summary of the Invention

[0006] The purpose of the present invention is to solve the problems of excessive SNP sites in the identification of apricot varieties in the existing technology and the lack of effective SNP sites that can be accurately used for the identification of different apricot varieties, so as to screen and identify a group of SNP-specific sites with high accuracy and most suitable for apricot germplasm identification. At the same time, the screening method and application thereof are provided, filling the gaps in the existing technology in the identification of apricot germplasm resources and genetic relationship research using only SNP technology.

[0007] In order to solve the above technical problems, the present invention is achieved through the following technical solutions.

[0008] The first aspect of the present invention provides the use of SNP sites in apricot variety identification, wherein the SNP sites are selected from one or more of the following:

[0009] 1. CAEKDK010000001.1_10440254, whose deoxynucleotide sequence is A / T;

[0010] 2. CAEKDK010000001.1_13568267, whose deoxynucleotide sequence is A / T;

[0011] 3. CAEKDK010000001.1_20639545, whose deoxynucleotide sequence is C / T;

[0012] 4. CAEKDK010000001.1_21909800, whose deoxynucleotide sequence is A / G;

[0013] 5. CAEKDK010000001.1_25411117, whose deoxynucleotide sequence is C / T;

[0014] 6. CAEKDK010000001.1_36367780, whose deoxynucleotide sequence is A / G;

[0015] 7. CAEKDK010000001.1_41251119, whose deoxynucleotide sequence is A / G;

[0016] 8. CAEKDK010000002.1_18290549, whose deoxynucleotide sequence is C / T;

[0017] 9. CAEKDK010000002.1_18928945, whose deoxynucleotide sequence is A / G;

[0018] 10. CAEKDK010000002.1_22722929, whose deoxynucleotide sequence is C / T;

[0019] 11. CAEKDK010000002.1_28105504, whose deoxynucleotide sequence is C / T;

[0020] 12. CAEKDK010000003.1_675221, whose deoxynucleotide sequence is A / G;

[0021] 13. CAEKDK010000003.1_15070082, whose deoxynucleotide sequence is C / T;

[0022] 14. CAEKDK010000003.1_21123450, whose deoxynucleotide sequence is A / C;

[0023] 15. CAEKDK010000003.1_25439844, whose deoxynucleotide sequence is A / T;

[0024] 16. CAEKDK010000004.1_7586379, whose deoxynucleotide sequence is A / G;

[0025] 17. CAEKDK010000004.1_19240527, whose deoxynucleotide sequence is C / T;

[0026] 18. CAEKDK010000004.1_22531282, whose deoxynucleotide sequence is A / G;

[0027] 19. CAEKDK010000005.1_12669636, whose deoxynucleotide sequence is C / T;

[0028] 20. CAEKDK010000005.1_13466650, whose deoxynucleotide sequence is A / G;

[0029] 21. CAEKDK010000005.1_17572886, whose deoxynucleotide sequence is C / T;

[0030] 22. CAEKDK010000006.1_4798304, whose deoxynucleotide sequence is A / C;

[0031] 23. CAEKDK010000006.1_5750123, whose deoxynucleotide sequence is C / T;

[0032] 24. CAEKDK010000006.1_20454043, whose deoxynucleotide sequence is C / G;

[0033] 25. CAEKDK010000006.1_21304403, whose deoxynucleotide sequence is C / G;

[0034] 26. CAEKDK010000007.1_10365019, whose deoxynucleotide sequence is A / G;

[0035] 27. CAEKDK010000008.1_5584589, whose deoxynucleotide sequence is C / T;

[0036] 28. CAEKDK010000008.1_7399902, whose deoxynucleotide sequence is A / G;

[0037] 29. CAEKDK010000008.1_15579729, whose deoxynucleotide sequence is C / T.

[0038] Preferably, the SNP site is screened by the following method:

[0039] (1) Collect apricot samples and extract total DNA;

[0040] (2) Whole genome sequencing of the extracted total DNA;

[0041] (3) Screen the sequencing results for SNP-specific sites.

[0042] Preferably, the apricots in step (1) are selected from Xinjiang apricots.

[0043] Preferably, the sample in step (1) is selected from one or more of apricot leaves, fruits, roots, and stems; most preferably, the sample is selected from apricot leaves.

[0044] Preferably, in step (1), the CTBA method is used to extract total DNA.

[0045] As a preference, after the total DNA is extracted in step (1), the DNA concentration and purity should be determined to control the DNA OD 260 / OD 280 It is 1.7-2.0.

[0046] Preferably, the SNP-specific site screening in step (3) specifically includes the following steps:

[0047] ① Using the fully sequenced apricot (Prunus armeniaca L.) genome (gene accession number GCA_903112645.1, https: / / www.ncbi.nlm.nih.gov / assembly / GCA_903112645.1# / def) as a reference sequence, we assembled and detected variants of the whole-genome sequencing data of apricot germplasm samples to generate a SNP library.

[0048] ②Preliminary screening of the SNP library in step ① was performed under the following conditions: 50 bp sequence conservation before and after the SNP site, biallelicity, missing rate < 0.05, minor allele frequency (MAF) corresponding to the SNP site > 0.3, and minimum sample depth ≥ 10X;

[0049] ③ After aligning the 100bp sequence before and after the SNP site with the apricot genome reference sequence, the SNP sites with specificity within 51-100bp before and after the site were screened out;

[0050] ④ For the SNP sites screened in step ③, a perl model was constructed for calculation, and the minimum combination of SNP sites that could completely distinguish different apricot germplasms was used as a benchmark to screen out SNP sites with 29 markers.

[0051] The second aspect of the present invention provides an application of SNP sites in studying the genetic diversity of apricot germplasm resources, wherein the SNP sites are selected from one or more of the following:

[0052] 1. CAEKDK010000001.1_10440254, whose deoxynucleotide sequence is A / T;

[0053] 2. CAEKDK010000001.1_13568267, whose deoxynucleotide sequence is A / T;

[0054] 3. CAEKDK010000001.1_20639545, whose deoxynucleotide sequence is C / T;

[0055] 4. CAEKDK010000001.1_21909800, whose deoxynucleotide sequence is A / G;

[0056] 5. CAEKDK010000001.1_25411117, whose deoxynucleotide sequence is C / T;

[0057] 6. CAEKDK010000001.1_36367780, whose deoxynucleotide sequence is A / G;

[0058] 7. CAEKDK010000001.1_41251119, whose deoxynucleotide sequence is A / G;

[0059] 8. CAEKDK010000002.1_18290549, whose deoxynucleotide sequence is C / T;

[0060] 9. CAEKDK010000002.1_18928945, whose deoxynucleotide sequence is A / G;

[0061] 10. CAEKDK010000002.1_22722929, whose deoxynucleotide sequence is C / T;

[0062] 11. CAEKDK010000002.1_28105504, whose deoxynucleotide sequence is C / T;

[0063] 12. CAEKDK010000003.1_675221, whose deoxynucleotide sequence is A / G;

[0064] 13. CAEKDK010000003.1_15070082, whose deoxynucleotide sequence is C / T;

[0065] 14. CAEKDK010000003.1_21123450, whose deoxynucleotide sequence is A / C;

[0066] 15. CAEKDK010000003.1_25439844, whose deoxynucleotide sequence is A / T;

[0067] 16. CAEKDK010000004.1_7586379, whose deoxynucleotide sequence is A / G;

[0068] 17. CAEKDK010000004.1_19240527, whose deoxynucleotide sequence is C / T;

[0069] 18. CAEKDK010000004.1_22531282, whose deoxynucleotide sequence is A / G;

[0070] 19. CAEKDK010000005.1_12669636, whose deoxynucleotide sequence is C / T;

[0071] 20. CAEKDK010000005.1_13466650, whose deoxynucleotide sequence is A / G;

[0072] 21. CAEKDK010000005.1_17572886, whose deoxynucleotide sequence is C / T;

[0073] 22. CAEKDK010000006.1_4798304, whose deoxynucleotide sequence is A / C;

[0074] 23. CAEKDK010000006.1_5750123, whose deoxynucleotide sequence is C / T;

[0075] 24. CAEKDK010000006.1_20454043, whose deoxynucleotide sequence is C / G;

[0076] 25. CAEKDK010000006.1_21304403, whose deoxynucleotide sequence is C / G;

[0077] 26. CAEKDK010000007.1_10365019, whose deoxynucleotide sequence is A / G;

[0078] 27. CAEKDK010000008.1_5584589, whose deoxynucleotide sequence is C / T;

[0079] 28. CAEKDK010000008.1_7399902, whose deoxynucleotide sequence is A / G;

[0080] 29. CAEKDK010000008.1_15579729, whose deoxynucleotide sequence is C / T.

[0081] Preferably, the research on the diversity of apricot germplasm genetic resources specifically includes the following steps:

[0082] ① Using the fully sequenced apricot (Prunus armeniaca L.) genome (accession number GCA_903112645.1, https: / / www.ncbi.nlm.nih.gov / assembly / GCA_903112645.1# / def) as a reference sequence, nuclear genome single nucleotide polymorphisms (SNPs) were extracted based on the whole genome sequencing data of apricot samples.

[0083] ②BWA (Burrows-Wheeler Aligner) software was used for sequencing, Picard software was used for deduplication, and SAMtools software was used for identification of SNP sites;

[0084] ③ Use VCFtools to evaluate the sequencing depth and quality value of SNPs and filter to obtain high-quality SNP sites;

[0085] ④ Use MEGA software to construct a whole-genome NeighborJoining evolutionary tree to analyze the phylogenetic relationship and kinship between different germplasms.

[0086] The third aspect of the present invention provides the use of SNP sites in apricot germplasm-assisted breeding, wherein the SNP sites are selected from one or more of the following:

[0087] 1. CAEKDK010000001.1_10440254, whose deoxynucleotide sequence is A / T;

[0088] 2. CAEKDK010000001.1_13568267, whose deoxynucleotide sequence is A / T;

[0089] 3. CAEKDK010000001.1_20639545, whose deoxynucleotide sequence is C / T;

[0090] 4. CAEKDK010000001.1_21909800, whose deoxynucleotide sequence is A / G;

[0091] 5. CAEKDK010000001.1_25411117, whose deoxynucleotide sequence is C / T;

[0092] 6. CAEKDK010000001.1_36367780, whose deoxynucleotide sequence is A / G;

[0093] 7. CAEKDK010000001.1_41251119, whose deoxynucleotide sequence is A / G;

[0094] 8. CAEKDK010000002.1_18290549, whose deoxynucleotide sequence is C / T;

[0095] 9. CAEKDK010000002.1_18928945, whose deoxynucleotide sequence is A / G;

[0096] 10. CAEKDK010000002.1_22722929, whose deoxynucleotide sequence is C / T;

[0097] 11. CAEKDK010000002.1_28105504, whose deoxynucleotide sequence is C / T;

[0098] 12. CAEKDK010000003.1_675221, whose deoxynucleotide sequence is A / G;

[0099] 13. CAEKDK010000003.1_15070082, whose deoxynucleotide sequence is C / T;

[0100] 14. CAEKDK010000003.1_21123450, whose deoxynucleotide sequence is A / C;

[0101] 15. CAEKDK010000003.1_25439844, whose deoxynucleotide sequence is A / T;

[0102] 16. CAEKDK010000004.1_7586379, whose deoxynucleotide sequence is A / G;

[0103] 17. CAEKDK010000004.1_19240527, whose deoxynucleotide sequence is C / T;

[0104] 18. CAEKDK010000004.1_22531282, whose deoxynucleotide sequence is A / G;

[0105] 19. CAEKDK010000005.1_12669636, whose deoxynucleotide sequence is C / T;

[0106] 20. CAEKDK010000005.1_13466650, whose deoxynucleotide sequence is A / G;

[0107] 21. CAEKDK010000005.1_17572886, whose deoxynucleotide sequence is C / T;

[0108] 22. CAEKDK010000006.1_4798304, whose deoxynucleotide sequence is A / C;

[0109] 23. CAEKDK010000006.1_5750123, whose deoxynucleotide sequence is C / T;

[0110] 24. CAEKDK010000006.1_20454043, whose deoxynucleotide sequence is C / G;

[0111] 25. CAEKDK010000006.1_21304403, whose deoxynucleotide sequence is C / G;

[0112] 26. CAEKDK010000007.1_10365019, whose deoxynucleotide sequence is A / G;

[0113] 27. CAEKDK010000008.1_5584589, whose deoxynucleotide sequence is C / T;

[0114] 28. CAEKDK010000008.1_7399902, whose deoxynucleotide sequence is A / G;

[0115] 29. CAEKDK010000008.1_15579729, whose deoxynucleotide sequence is C / T.

[0116] It should be understood that in the context of the present invention, unless otherwise specified, the software used, such as Velvet, Plastome, Reputer, mVISTA, MrBayes, IRscope, Dnasp, MEGA7.0, Burrows-WheelerAligner, Picard, SAMtools, VCFtools, etc., are all software or tools known or commonly used in the art. Those skilled in the art are capable of using them normally according to the instructions or user guides provided by the software suppliers, and screening related genes or sequences according to the parameters or conditions provided by the software.

[0117] Given the stability and effectiveness of the SNP method, the International Union for the Protection of Plant Varieties Rights (UPOV) has identified SSR and SNP as marker methods for constructing DNA fingerprint databases in the draft BMT testing guidelines. Compared with SSR markers, SNPs have the characteristics of strong targeting, rich sources of variation, and huge potential numbers. Since most SNPs only have two base forms, they are considered diallelic markers, which has the advantage of simplifying genotyping methods and subsequent digital encoding. Moreover, SNPs are variations in the nucleotides themselves and do not require reading the molecular weight. Sequencing methods are used to directly obtain allelic variation genotypes, which have high genetic stability and are more suitable for digital database construction. The use of SNP molecular markers to identify the molecular identity of crops will undoubtedly play a positive role in the management of crop variety resources, the protection, utilization and evaluation of crop varieties.

[0118] Through extensive research, this invention has screened and identified a set of highly accurate SNP-specific loci that are most suitable for apricot germplasm identification. By detecting and comparing SNP loci in apricot samples of unknown species, rapid and accurate identification of apricot varieties can be achieved. Furthermore, SNP-specific loci from different germplasms can be fully utilized as a supplement to DNA sequence combinations, enabling scientific and precise identification of apricot germplasms in germplasm nurseries and research on their genetic relationships.

[0119] The present invention has the following beneficial effects compared to the prior art:

[0120] (1) The present invention has established a scientific and standardized identification method for apricot germplasm resources based on SNP technology, which can facilitate the efficient classification and management of apricot plants. It has good specificity and can serve as a key credential for determining apricot species, filling the technical gap in the development of apricot SNP molecular markers in the existing technology.

[0121] (2) The present invention provides 29 SNP molecular markers suitable for apricot plants, which can achieve high-throughput operation, have extremely high accuracy, and simple data reading, which can save costs and improve efficiency. At the same time, it helps to guide the breeding of high-amino acid functional apricot variety resources with high medicinal value through molecular biology and bioinformatics methods in the later stage, and can provide theoretical support for traditional variety breeding methods, reduce research costs, and improve breeding efficiency.

[0122] (3) The 29 SNP-specific loci provided by this invention are of great significance for the scientific preservation, rapid identification, breeding, classification, and management of apricot fruit and vegetable resources. They help to solve the problem of frequent hybridization between different apricot species, the large number of transitional types, and the confusion caused by the classification of fruit tree resources. They can quickly identify and authenticate the specific species of apricot germplasm, playing a vital role in the preservation, breeding, and genetic diversity research of apricot fruit tree resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0123] Figure 1 This is a schematic diagram of the information results of 29 SNP sites in 20 apricot varieties including Kantaile apricot.

[0124] Figure 2 This is a schematic diagram of the information results of 29 SNP sites in 20 apricot varieties including Danxing.

[0125] Figure 3 This is a schematic diagram of the information results of 29 SNP sites in 20 apricot varieties including Wang Enmao No. 1 apricot.

[0126] Figure 4 This is a schematic diagram of the information results of 29 SNP sites in 20 apricot varieties including Mayimake Yulike apricot.

[0127] Figure 5 This is a schematic diagram of the information results of 29 SNP sites in 20 apricot varieties including Akdarez apricot.

[0128] Figure 6 This is a schematic diagram of the information results of 29 SNP sites in 20 apricot varieties including Asibi Ke apricot.

[0129] Figure 7 This is a schematic diagram of the information results of 29 SNP sites in 9 apricot varieties including Akexing.

[0130] Figure 8This is a schematic diagram of the results of genetic resource diversity research on 129 apricot germplasm resources. DETAILED DESCRIPTION

[0131] In order to make the purpose, technical solution and effect of the present invention clearer and more specific, the present invention is further described in detail with reference to the following examples. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0132] Unless otherwise specified, the samples, consumables, and reagents listed or used in the present invention are all commercially available. The experimental methods used in the present invention, such as DNA extraction and whole genome sequencing, are conventional methods and techniques in the art.

[0133] Example 1

[0134] Screening of SNP-specific sites in apricot germplasm includes the following steps:

[0135] (1) Collect fresh apricot leaf samples and extract total DNA (Plant Genomic DNA Extraction Kit, Beijing Tiangen Biotechnology Co., Ltd.):

[0136] ① Take 200 mg of leaf tissue and place it in a crushing tube, soak it in liquid nitrogen, and grind it into powder using a machine;

[0137] ② Add appropriate amount of CTAB lysis buffer and mix well with a vortex mixer;

[0138] ③ Place in a constant temperature mixer and incubate at 65°C for 60 min;

[0139] ④ After lysis is complete, cool the tube and centrifuge at 10,000 rpm for 5 minutes at room temperature. Aspirate the remaining liquid and transfer it to a 2.0 mL centrifuge tube. Add an equal volume of Tris-saturated phenol / chloroform / isoamyl alcohol mixed solvent (the volume ratio of the three is 25:24:1) and mix by inverting 5-10 times.

[0140] ⑤ Centrifuge at 10,000 rpm for 10 min at room temperature, aspirate the supernatant, and transfer to a 1.5 mL centrifuge tube;

[0141] ⑥ Add 2 / 3 volume of -20℃ pre-cooled isopropanol, mix well by inverting 3-5 times, and place in a -20℃ refrigerator to settle for more than 2 hours;

[0142] ⑦ Centrifuge at 12000 rpm for 15 min at room temperature, aspirate the supernatant, add 750 μL 75% ethanol, and wash the precipitate with a pipette;

[0143] ⑧ Centrifuge at 12000 rpm for 3 minutes at room temperature, aspirate the supernatant, centrifuge briefly to remove the residual liquid, dry for 3-5 minutes, and dissolve in an appropriate amount of TE solution;

[0144] ⑨ DNA concentration and purity were measured using a NanoDrop 1000 spectrophotometer (Themo Scientific) UV spectrophotometer. 260 / OD 280 1.7-2.0 meets the requirements;

[0145] (2) Whole genome sequencing was performed on the extracted total DNA of the sample using the MGISEQ-2000 sequencing instrument with a sequencing depth of 20×. The specific steps included:

[0146] ① Sample testing: Sample testing includes sample concentration, integrity, and purity; concentration is determined by fluorescence quantification or microplate reader; and sample integrity and purity are tested using agarose gel (agarose gel concentration: 1%, voltage: 150V, electrophoresis time: 40min);

[0147] ② Sample shearing: Take 1 μg of genomic DNA sample and perform ultrasonic shearing;

[0148] ③ Fragment size selection: Fragment selection is performed on the sheared sample magnetic beads so that the sample bands are concentrated around 200-400bp;

[0149] ④ End repair, adding "A", and adapter ligation: Prepare the reaction system, react at room temperature for a certain period of time, repair the double-stranded cDNA ends, add the A base at the 3' end, prepare the adapter ligation reaction system, react at room temperature for a certain period of time, and then connect the adapter to the DNA;

[0150] ⑤PCR reaction and product recovery: prepare the PCR reaction system and set the reaction program to amplify the ligation product; the amplified product is purified and recovered using magnetic beads;

[0151] ⑥ PCR product cyclization: After the PCR product is denatured into a single strand, a cyclization reaction system is prepared. After sufficient mixing and reaction at room temperature for a period of time, a single-stranded circular product is obtained, and the uncyclized linear DNA molecules are digested;

[0152] ⑦ Library testing and sequencing: The circularized product undergoes a concentration test before being loaded onto the sequencing machine. Qualified libraries are then scheduled for sequencing (DEBSEQ): Single-stranded circular DNA molecules are replicated via roller rings to form a DNA nanoball (DNB) containing over 300 copies. The resulting DNBs are loaded into the mesh pores of a high-density DNA nanochip and sequenced using combined probe anchor polymerization (cPAS). The resulting data are then quality-controlled using FASTQC software.

[0153] (3) Screening the sequencing results for SNP-specific sites includes the following steps:

[0154] ① Using the fully sequenced apricot (Prunus armeniaca L.) genome (gene accession number GCA_903112645.1, https: / / www.ncbi.nlm.nih.gov / assembly / GCA_903112645.1# / def) as the reference sequence, we assembled and detected variants using BWA, SAMTOOLS, and GATK based on the whole-genome sequencing data of apricot germplasm samples, generating a screening library of 19,065,246 SNP markers.

[0155] ② The SNP library in step ① was preliminarily screened under the following conditions: 50 bp sequence conservation before and after the SNP site, biallelicity, missing rate < 0.05, minor allele frequency (MAF) corresponding to the SNP site > 0.3, and minimum sample depth ≥ 10X, resulting in 1707 SNP sites.

[0156] ③ After aligning the 100 bp sequences before and after the 1707 SNP sites obtained in step ② with the apricot genome reference sequence, 1548 high-quality SNP sites with specificity within 51-100 bp before and after the sites were screened out;

[0157] ④ Of the 1548 high-quality SNP sites screened in step ②, there are 1198 SNP sites that can be successfully used. The perl model was used to calculate these 1198 SNP sites. Based on the minimum SNP site combination that can completely distinguish 129 apricot germplasms, 29 SNP sites with markers were screened, which are as follows:

[0158] 1. CAEKDK010000001.1_10440254, whose deoxynucleotide sequence is A / T;

[0159] 2. CAEKDK010000001.1_13568267, whose deoxynucleotide sequence is A / T;

[0160] 3. CAEKDK010000001.1_20639545, whose deoxynucleotide sequence is C / T;

[0161] 4. CAEKDK010000001.1_21909800, whose deoxynucleotide sequence is A / G;

[0162] 5. CAEKDK010000001.1_25411117, whose deoxynucleotide sequence is C / T;

[0163] 6. CAEKDK010000001.1_36367780, whose deoxynucleotide sequence is A / G;

[0164] 7. CAEKDK010000001.1_41251119, whose deoxynucleotide sequence is A / G;

[0165] 8. CAEKDK010000002.1_18290549, whose deoxynucleotide sequence is C / T;

[0166] 9. CAEKDK010000002.1_18928945, whose deoxynucleotide sequence is A / G;

[0167] 10. CAEKDK010000002.1_22722929, whose deoxynucleotide sequence is C / T;

[0168] 11. CAEKDK010000002.1_28105504, whose deoxynucleotide sequence is C / T;

[0169] 12. CAEKDK010000003.1_675221, whose deoxynucleotide sequence is A / G;

[0170] 13. CAEKDK010000003.1_15070082, whose deoxynucleotide sequence is C / T;

[0171] 14. CAEKDK010000003.1_21123450, whose deoxynucleotide sequence is A / C;

[0172] 15. CAEKDK010000003.1_25439844, whose deoxynucleotide sequence is A / T;

[0173] 16. CAEKDK010000004.1_7586379, whose deoxynucleotide sequence is A / G;

[0174] 17. CAEKDK010000004.1_19240527, whose deoxynucleotide sequence is C / T;

[0175] 18. CAEKDK010000004.1_22531282, whose deoxynucleotide sequence is A / G;

[0176] 19. CAEKDK010000005.1_12669636, whose deoxynucleotide sequence is C / T;

[0177] 20. CAEKDK010000005.1_13466650, whose deoxynucleotide sequence is A / G;

[0178] 21. CAEKDK010000005.1_17572886, whose deoxynucleotide sequence is C / T;

[0179] 22. CAEKDK010000006.1_4798304, whose deoxynucleotide sequence is A / C;

[0180] 23. CAEKDK010000006.1_5750123, whose deoxynucleotide sequence is C / T;

[0181] 24. CAEKDK010000006.1_20454043, whose deoxynucleotide sequence is C / G;

[0182] 25. CAEKDK010000006.1_21304403, whose deoxynucleotide sequence is C / G;

[0183] 26. CAEKDK010000007.1_10365019, whose deoxynucleotide sequence is A / G;

[0184] 27. CAEKDK010000008.1_5584589, whose deoxynucleotide sequence is C / T;

[0185] 28. CAEKDK010000008.1_7399902, whose deoxynucleotide sequence is A / G;

[0186] 29, CAEKDK010000008.1_15579729, whose deoxynucleotide sequence is C / T;

[0187] Example 2

[0188] The determination of 29 SNPs in different apricot varieties included the following steps:

[0189] (1) Healthy, clean, and fresh apricot leaves of 129 different varieties were collected from the germplasm resource garden. 1-2 g of each sample was sampled and total DNA was extracted (Plant Genomic DNA Extraction Kit, Beijing Tiangen Biotechnology Co., Ltd.):

[0190] ① Take 200 mg of leaf tissue and place it in a crushing tube, soak it in liquid nitrogen, and grind it into powder using a machine;

[0191] ② Add appropriate amount of CTAB lysis buffer and mix well with a vortex mixer;

[0192] ③ Place in a constant temperature mixer and incubate at 65°C for 60 min;

[0193] ④ After lysis is complete, cool the tube and centrifuge at 10,000 rpm for 5 minutes at room temperature. Aspirate the remaining liquid and transfer it to a 2.0 mL centrifuge tube. Add an equal volume of Tris-saturated phenol / chloroform / isoamyl alcohol mixed solvent (the volume ratio of the three is 25:24:1) and mix by inverting 5-10 times.

[0194] ⑤ Centrifuge at 10,000 rpm for 10 min at room temperature, aspirate the supernatant, and transfer to a 1.5 mL centrifuge tube;

[0195] ⑥ Add 2 / 3 volume of -20℃ pre-cooled isopropanol, mix well by inverting 3-5 times, and place in a -20℃ refrigerator to settle for more than 2 hours;

[0196] ⑦ Centrifuge at 12000 rpm for 15 min at room temperature, aspirate the supernatant, add 750 μL 75% ethanol, and wash the precipitate with a pipette;

[0197] ⑧ Centrifuge at 12000 rpm for 3 minutes at room temperature, aspirate the supernatant, centrifuge briefly to remove the residual liquid, dry for 3-5 minutes, and dissolve in an appropriate amount of TE solution;

[0198] ⑨ DNA concentration and purity were measured using a NanoDrop 1000 spectrophotometer (Themo Scientific) UV spectrophotometer. 260 / OD 280 1.7-2.0 meets the requirements;

[0199] (2) Whole genome sequencing was performed on the extracted total DNA of each sample using an MGISEQ-2000 sequencing instrument with a sequencing depth of 20×. The specific steps included:

[0200] ① Sample testing: Sample testing includes sample concentration, integrity, and purity; concentration is determined by fluorescence quantification or microplate reader; and sample integrity and purity are tested using agarose gel (agarose gel concentration: 1%, voltage: 150V, electrophoresis time: 40min);

[0201] ② Sample shearing: Take 1 μg of genomic DNA sample and perform ultrasonic shearing;

[0202] ③ Fragment size selection: Fragment selection is performed on the sheared sample magnetic beads so that the sample bands are concentrated around 200-400bp;

[0203] ④ End repair, adding "A", and adapter ligation: Prepare the reaction system, react at room temperature for a certain period of time, repair the double-stranded cDNA ends, add the A base at the 3' end, prepare the adapter ligation reaction system, react at room temperature for a certain period of time, and then connect the adapter to the DNA;

[0204] ⑤PCR reaction and product recovery: prepare the PCR reaction system and set the reaction program to amplify the ligation product; the amplified product is purified and recovered using magnetic beads;

[0205] ⑥ PCR product cyclization: After the PCR product is denatured into a single strand, a cyclization reaction system is prepared. After sufficient mixing and reaction at room temperature for a period of time, a single-stranded circular product is obtained, and the uncyclized linear DNA molecules are digested;

[0206] ⑦ Library testing and sequencing: The circularized product undergoes a concentration test before being loaded onto the sequencing machine. Qualified libraries are then scheduled for sequencing (DEBSEQ): Single-stranded circular DNA molecules are replicated via roller rings to form a DNA nanoball (DNB) containing over 300 copies. The resulting DNBs are loaded into the mesh pores of a high-density DNA nanochip and sequenced using combined probe anchor polymerization (cPAS). The resulting data are then quality-controlled using FASTQC software.

[0207] (3) Perform SNP analysis on sequencing results:

[0208] ① Using the whole-genome sequenced apricot (Prunus armeniaca L.) genome (gene accession number GCA_903112645.1, https: / / www.ncbi.nlm.nih.gov / assembly / GCA_903112645.1# / def) as the reference sequence, extract nuclear genome single nucleotide polymorphisms (SNPs) based on the whole-genome sequencing data in step (2);

[0209] ②BWA (Burrows-Wheeler Aligner) software was used for sequencing, Picard software was used for deduplication, and SAMtools software was used for identification of SNP sites;

[0210] ③ Compare the SNP site results of each sample with the 29 SNP-specific sites in Example 1 to clarify the number and type of SNP site mutations. The comparison results are as follows: Figure 1-7 As shown, it can be seen that the marker combinations of different apricot germplasms can be completely distinguished by using the above 29 SNP sites.

[0211] Furthermore, based on the above results, a genetic resource diversity study was conducted on 129 apricot germplasm resources, specifically including the following steps:

[0212] ① Using the whole-genome sequenced apricot (Prunus armeniaca L.) genome (gene accession number GCA_903112645.1, https: / / www.ncbi.nlm.nih.gov / assembly / GCA_903112645.1# / def) as a reference sequence, nuclear genome single nucleotide polymorphisms (SNPs) were extracted based on the whole-genome sequencing data in Example 1-2;

[0213] ②BWA (Burrows-Wheeler Aligner) software was used for sequencing, Picard software was used for deduplication, and SAMtools software was used for identification of SNP sites;

[0214] ③ Use VCFtools to evaluate the sequencing depth and quality value of SNPs and filter to obtain high-quality SNP sites;

[0215] ④ The whole genome NeighborJoining evolutionary tree was constructed using MEGA software to analyze the phylogenetic relationship and phylogenetic relationship between different germplasms; the results are as follows Figure 8 As shown in the phylogenetic tree, the different subpopulations are generally clustered together, with the seven accessions from the Pop3 subpopulation clustering completely together. Accessions numbered 46, 87, 102, 104, and 110 from the Pop2 subpopulation interspersed with the Pop1 subpopulation, confirming that these accessions contain a significant amount of Pop1 subpopulation ancestry as shown in the structural analysis. Accessions numbered 36, 77, and 90 from the Pop1 subpopulation interspersed with the Pop2 subpopulation, confirming that these accessions contain a significant amount of Pop2 subpopulation ancestry as shown in the structural analysis.

[0216] Example 3

[0217] The identification of apricot varieties using SNP loci includes the following steps:

[0218] (1) Obtain a healthy, clean, and fresh apricot leaf sample, numbered Y1, and sample 2 g for total DNA extraction (Plant Genomic DNA Extraction Kit, Beijing Tiangen Biotechnology Co., Ltd.):

[0219] ① Take 200 mg of leaf tissue and place it in a crushing tube, soak it in liquid nitrogen, and grind it into powder using a machine;

[0220] ② Add appropriate amount of CTAB lysis buffer and mix well with a vortex mixer;

[0221] ③ Place in a constant temperature mixer and incubate at 65°C for 60 min;

[0222] ④ After lysis is complete, cool the tube and centrifuge at 10,000 rpm for 5 minutes at room temperature. Aspirate the remaining liquid and transfer it to a 2.0 mL centrifuge tube. Add an equal volume of Tris-saturated phenol / chloroform / isoamyl alcohol mixed solvent (the volume ratio of the three is 25:24:1) and mix by inverting 5-10 times.

[0223] ⑤ Centrifuge at 10,000 rpm for 10 min at room temperature, aspirate the supernatant, and transfer to a 1.5 mL centrifuge tube;

[0224] ⑥ Add 2 / 3 volume of -20℃ pre-cooled isopropanol, mix well by inverting 3-5 times, and place in a -20℃ refrigerator to settle for more than 2 hours;

[0225] ⑦ Centrifuge at 12000 rpm for 15 min at room temperature, aspirate the supernatant, add 750 μL 75% ethanol, and wash the precipitate with a pipette;

[0226] ⑧ Centrifuge at 12000 rpm for 3 minutes at room temperature, aspirate the supernatant, centrifuge briefly to remove the residual liquid, dry for 3-5 minutes, and dissolve in an appropriate amount of TE solution;

[0227] ⑨ DNA concentration and purity were measured using a NanoDrop 1000 spectrophotometer (Themo Scientific) UV spectrophotometer. 260 / OD 280 is 1.8;

[0228] (2) Whole genome sequencing was performed on the extracted total DNA of the sample using the MGISEQ-2000 sequencing instrument with a sequencing depth of 20×. The specific steps included:

[0229] ① Sample testing: Sample testing includes sample concentration, integrity, and purity; concentration is determined by fluorescence quantification or microplate reader; and sample integrity and purity are tested using agarose gel (agarose gel concentration: 1%, voltage: 150V, electrophoresis time: 40min);

[0230] ② Sample shearing: Take 1 μg of genomic DNA sample and perform ultrasonic shearing;

[0231] ③ Fragment size selection: Fragment selection is performed on the sheared sample magnetic beads so that the sample bands are concentrated around 200-400bp;

[0232] ④ End repair, adding "A", and adapter ligation: Prepare the reaction system, react at room temperature for a certain period of time, repair the double-stranded cDNA ends, add the A base at the 3' end, prepare the adapter ligation reaction system, react at room temperature for a certain period of time, and then connect the adapter to the DNA;

[0233] ⑤PCR reaction and product recovery: prepare the PCR reaction system and set the reaction program to amplify the ligation product; the amplified product is purified and recovered using magnetic beads;

[0234] ⑥ PCR product cyclization: After the PCR product is denatured into a single strand, a cyclization reaction system is prepared. After sufficient mixing and reaction at room temperature for a period of time, a single-stranded circular product is obtained, and the uncyclized linear DNA molecules are digested;

[0235] ⑦ Library testing and sequencing: The circularized product undergoes a concentration test before being loaded onto the sequencing machine. Qualified libraries are then scheduled for sequencing (DEBSEQ): Single-stranded circular DNA molecules are replicated via roller rings to form a DNA nanoball (DNB) containing over 300 copies. The resulting DNBs are loaded into the mesh pores of a high-density DNA nanochip and sequenced using combined probe anchor polymerization (cPAS). The resulting data are then quality-controlled using FASTQC software.

[0236] (3) Perform SNP analysis on sequencing results:

[0237] ① Using the whole-genome sequenced apricot (Prunus armeniaca L.) genome (gene accession number GCA_903112645.1, https: / / www.ncbi.nlm.nih.gov / assembly / GCA_903112645.1# / def) as the reference sequence, extract nuclear genome single nucleotide polymorphisms (SNPs) based on the whole-genome sequencing data in step (2);

[0238] ②BWA (Burrows-Wheeler Aligner) software was used for sequencing, Picard software was used for deduplication, and SAMtools software was used for identification of SNP sites;

[0239] ③The SNP site information of this sample is as follows:

[0240] 1. CAEKDK010000001.1_10440254, whose deoxynucleotide sequence is A / A;

[0241] 2. CAEKDK010000001.1_13568267, whose deoxynucleotide sequence is A / A;

[0242] 3. CAEKDK010000001.1_20639545, whose deoxynucleotide sequence is C / T;

[0243] 4. CAEKDK010000001.1_21909800, whose deoxynucleotide sequence is A / G;

[0244] 5. CAEKDK010000001.1_25411117, whose deoxynucleotide sequence is C / C;

[0245] 6. CAEKDK010000001.1_36367780, whose deoxynucleotide sequence is G / A;

[0246] 7. CAEKDK010000001.1_41251119, whose deoxynucleotide sequence is G / G;

[0247] 8. CAEKDK010000002.1_18290549, whose deoxynucleotide sequence is C / T;

[0248] 9. CAEKDK010000002.1_18928945, whose deoxynucleotide sequence is A / G;

[0249] 10. CAEKDK010000002.1_22722929, whose deoxynucleotide sequence is T / C;

[0250] 11. CAEKDK010000002.1_28105504, whose deoxynucleotide sequence is T / T;

[0251] 12. CAEKDK010000003.1_675221, whose deoxynucleotide sequence is A / A;

[0252] 13. CAEKDK010000003.1_15070082, whose deoxynucleotide sequence is T / T;

[0253] 14. CAEKDK010000003.1_21123450, whose deoxynucleotide sequence is N / N;

[0254] 15. CAEKDK010000003.1_25439844, whose deoxynucleotide sequence is A / A;

[0255] 16. CAEKDK010000004.1_7586379, whose deoxynucleotide sequence is A / G;

[0256] 17. CAEKDK010000004.1_19240527, whose deoxynucleotide sequence is C / C;

[0257] 18. CAEKDK010000004.1_22531282, whose deoxynucleotide sequence is G / A;

[0258] 19. CAEKDK010000005.1_12669636, whose deoxynucleotide sequence is C / T;

[0259] 20. CAEKDK010000005.1_13466650, whose deoxynucleotide sequence is G / G;

[0260] 21. CAEKDK010000005.1_17572886, whose deoxynucleotide sequence is C / T;

[0261] 22. CAEKDK010000006.1_4798304, whose deoxynucleotide sequence is C / C;

[0262] 23. CAEKDK010000006.1_5750123, whose deoxynucleotide sequence is T / T;

[0263] 24. CAEKDK010000006.1_20454043, whose deoxynucleotide sequence is C / C;

[0264] 25. CAEKDK010000006.1_21304403, whose deoxynucleotide sequence is G / G;

[0265] 26. CAEKDK010000007.1_10365019, whose deoxynucleotide sequence is G / G;

[0266] 27. CAEKDK010000008.1_5584589, whose deoxynucleotide sequence is T / T;

[0267] 28. CAEKDK010000008.1_7399902, whose deoxynucleotide sequence is G / A;

[0268] 29. CAEKDK010000008.1_15579729, whose deoxynucleotide sequence is T / T.

[0269] By comparing the above results with those in Example 2, it can be clearly seen that the species of the apricot sample is Akdarezi apricot.

[0270] Example 4

[0271] The identification of apricot varieties using SNP loci includes the following steps:

[0272] (1) Obtain a healthy, clean, and fresh apricot leaf sample, numbered Y2, and sample 2 g for total DNA extraction (Plant Genomic DNA Extraction Kit, Beijing Tiangen Biotechnology Co., Ltd.):

[0273] ① Take 200 mg of leaf tissue and place it in a crushing tube, soak it in liquid nitrogen, and grind it into powder using a machine;

[0274] ② Add appropriate amount of CTAB lysis buffer and mix well with a vortex mixer;

[0275] ③ Place in a constant temperature mixer and incubate at 65°C for 60 min;

[0276] ④ After lysis is complete, cool the tube and centrifuge at 10,000 rpm for 5 minutes at room temperature. Aspirate the remaining liquid and transfer it to a 2.0 mL centrifuge tube. Add an equal volume of Tris-saturated phenol / chloroform / isoamyl alcohol mixed solvent (the volume ratio of the three is 25:24:1) and mix by inverting 5-10 times.

[0277] ⑤ Centrifuge at 10,000 rpm for 10 min at room temperature, aspirate the supernatant, and transfer to a 1.5 mL centrifuge tube;

[0278] ⑥ Add 2 / 3 volume of -20℃ pre-cooled isopropanol, mix well by inverting 3-5 times, and place in a -20℃ refrigerator to settle for more than 2 hours;

[0279] ⑦ Centrifuge at 12000 rpm for 15 min at room temperature, aspirate the supernatant, add 750 μL 75% ethanol, and wash the precipitate with a pipette;

[0280] ⑧ Centrifuge at 12000 rpm for 3 minutes at room temperature, aspirate the supernatant, centrifuge briefly to remove the residual liquid, dry for 3-5 minutes, and dissolve in an appropriate amount of TE solution;

[0281] ⑨ DNA concentration and purity were measured using a NanoDrop 1000 spectrophotometer (Themo Scientific) UV spectrophotometer. 260 / OD 280 is 1.8;

[0282] (2) Whole genome sequencing was performed on the extracted total DNA of the sample using the MGISEQ-2000 sequencing instrument with a sequencing depth of 20×. The specific steps included:

[0283] ① Sample testing: Sample testing includes sample concentration, integrity, and purity; concentration is determined by fluorescence quantification or microplate reader; and sample integrity and purity are tested using agarose gel (agarose gel concentration: 1%, voltage: 150V, electrophoresis time: 40min);

[0284] ② Sample shearing: Take 1 μg of genomic DNA sample and perform ultrasonic shearing;

[0285] ③ Fragment size selection: Fragment selection is performed on the sheared sample magnetic beads so that the sample bands are concentrated around 200-400bp;

[0286] ④ End repair, adding "A", and adapter ligation: Prepare the reaction system, react at room temperature for a certain period of time, repair the double-stranded cDNA ends, add the A base at the 3' end, prepare the adapter ligation reaction system, react at room temperature for a certain period of time, and then connect the adapter to the DNA;

[0287] ⑤PCR reaction and product recovery: prepare the PCR reaction system and set the reaction program to amplify the ligation product; the amplified product is purified and recovered using magnetic beads;

[0288] ⑥ PCR product cyclization: After the PCR product is denatured into a single strand, a cyclization reaction system is prepared. After sufficient mixing and reaction at room temperature for a period of time, a single-stranded circular product is obtained, and the uncyclized linear DNA molecules are digested;

[0289] ⑦ Library testing and sequencing: The circularized product undergoes a concentration test before being loaded onto the sequencing machine. Qualified libraries are then scheduled for sequencing (DEBSEQ): Single-stranded circular DNA molecules are replicated via roller rings to form a DNA nanoball (DNB) containing over 300 copies. The resulting DNBs are loaded into the mesh pores of a high-density DNA nanochip and sequenced using combined probe anchor polymerization (cPAS). The resulting data are then quality-controlled using FASTQC software.

[0290] (3) Perform SNP analysis on sequencing results:

[0291] ① Using the whole-genome sequenced apricot (Prunus armeniaca L.) genome (gene accession number GCA_903112645.1, https: / / www.ncbi.nlm.nih.gov / assembly / GCA_903112645.1# / def) as the reference sequence, extract nuclear genome single nucleotide polymorphisms (SNPs) based on the whole-genome sequencing data in step (2);

[0292] ②BWA (Burrows-Wheeler Aligner) software was used for sequencing, Picard software was used for deduplication, and SAMtools software was used for identification of SNP sites;

[0293] ③The SNP site information of this sample is as follows:

[0294] 1. CAEKDK010000001.1_10440254, whose deoxynucleotide sequence is T / A;

[0295] 2. CAEKDK010000001.1_13568267, whose deoxynucleotide sequence is T / T;

[0296] 3. CAEKDK010000001.1_20639545, whose deoxynucleotide sequence is C / T;

[0297] 4. CAEKDK010000001.1_21909800, whose deoxynucleotide sequence is A / G;

[0298] 5. CAEKDK010000001.1_25411117, whose deoxynucleotide sequence is C / C;

[0299] 6. CAEKDK010000001.1_36367780, whose deoxynucleotide sequence is N / N;

[0300] 7. CAEKDK010000001.1_41251119, whose deoxynucleotide sequence is G / G;

[0301] 8. CAEKDK010000002.1_18290549, whose deoxynucleotide sequence is C / T;

[0302] 9. CAEKDK010000002.1_18928945, whose deoxynucleotide sequence is G / G;

[0303] 10. CAEKDK010000002.1_22722929, whose deoxynucleotide sequence is T / T;

[0304] 11. CAEKDK010000002.1_28105504, whose deoxynucleotide sequence is T / C;

[0305] 12. CAEKDK010000003.1_675221, whose deoxynucleotide sequence is G / A;

[0306] 13. CAEKDK010000003.1_15070082, whose deoxynucleotide sequence is T / T;

[0307] 14. CAEKDK010000003.1_21123450, whose deoxynucleotide sequence is C / C;

[0308] 15. CAEKDK010000003.1_25439844, whose deoxynucleotide sequence is A / A;

[0309] 16. CAEKDK010000004.1_7586379, whose deoxynucleotide sequence is A / A;

[0310] 17. CAEKDK010000004.1_19240527, whose deoxynucleotide sequence is C / C;

[0311] 18. CAEKDK010000004.1_22531282, whose deoxynucleotide sequence is G / A;

[0312] 19. CAEKDK010000005.1_12669636, whose deoxynucleotide sequence is C / C;

[0313] 20. CAEKDK010000005.1_13466650, whose deoxynucleotide sequence is A / G;

[0314] 21. CAEKDK010000005.1_17572886, whose deoxynucleotide sequence is C / T;

[0315] 22. CAEKDK010000006.1_4798304, whose deoxynucleotide sequence is C / A;

[0316] 23. CAEKDK010000006.1_5750123, whose deoxynucleotide sequence is T / T;

[0317] 24. CAEKDK010000006.1_20454043, whose deoxynucleotide sequence is G / G;

[0318] 25. CAEKDK010000006.1_21304403, whose deoxynucleotide sequence is C / G;

[0319] 26. CAEKDK010000007.1_10365019, whose deoxynucleotide sequence is G / A;

[0320] 27. CAEKDK010000008.1_5584589, whose deoxynucleotide sequence is T / T;

[0321] 28. CAEKDK010000008.1_7399902, whose deoxynucleotide sequence is G / A;

[0322] 29. CAEKDK010000008.1_15579729, whose deoxynucleotide sequence is T / T.

[0323] Comparing the above results with those in Example 2, it can be clearly seen that the species of the apricot sample is Yewu Tuyang apricot.

[0324] The above detailed description of the analytical methods involved in the present invention provides a detailed introduction. It should be noted that the above description is intended solely to help those skilled in the art better understand the methods and concepts of the present invention, and is not intended to limit the relevant content. Without departing from the principles of the present invention, those skilled in the art may make appropriate adjustments or modifications to the present invention, and such adjustments and modifications shall also fall within the scope of protection of the present invention.

Claims

1. Application of SNP loci in apricot variety identification, characterized in that: The SNP sites are composed of the following:

1. CAEKDK010000001.1_10440254, whose deoxynucleotide sequence is A / T; 2. CAEKDK010000001.1_13568267, whose deoxynucleotide sequence is A / T; 3. CAEKDK010000001.1_20639545, whose deoxynucleotide sequence is C / T; 4. CAEKDK010000001.1_21909800, whose deoxynucleotide sequence is A / G; 5. CAEKDK010000001.1_25411117, whose deoxynucleotide sequence is C / T; 6. CAEKDK010000001.1_36367780, whose deoxynucleotide sequence is A / G; 7. CAEKDK010000001.1_41251119, whose deoxynucleotide sequence is A / G; 8. CAEKDK010000002.1_18290549, whose deoxynucleotide sequence is C / T; 9. CAEKDK010000002.1_18928945, whose deoxynucleotide sequence is A / G; 10. CAEKDK010000002.1_22722929, whose deoxynucleotide sequence is C / T; 11. CAEKDK010000002.1_28105504, whose deoxynucleotide sequence is C / T; 12. CAEKDK010000003.1_675221, whose deoxynucleotide sequence is A / G; 13. CAEKDK010000003.1_15070082, whose deoxynucleotide sequence is C / T; 14. CAEKDK010000003.1_21123450, whose deoxynucleotide sequence is A / C; 15. CAEKDK010000003.1_25439844, whose deoxynucleotide sequence is A / T; 16. CAEKDK010000004.1_7586379, whose deoxynucleotide sequence is A / G; 17. CAEKDK010000004.1_19240527, whose deoxynucleotide sequence is C / T; 18. CAEKDK010000004.1_22531282, whose deoxynucleotide sequence is A / G; 19. CAEKDK010000005.1_12669636, whose deoxynucleotide sequence is C / T; 20. CAEKDK010000005.1_13466650, whose deoxynucleotide sequence is A / G; 21. CAEKDK010000005.1_17572886, whose deoxynucleotide sequence is C / T; 22. CAEKDK010000006.1_4798304, whose deoxynucleotide sequence is A / C; 23. CAEKDK010000006.1_5750123, whose deoxynucleotide sequence is C / T; 24. CAEKDK010000006.1_20454043, whose deoxynucleotide sequence is C / G; 25. CAEKDK010000006.1_21304403, whose deoxynucleotide sequence is C / G; 26. CAEKDK010000007.1_10365019, whose deoxynucleotide sequence is A / G; 27. CAEKDK010000008.1_5584589, whose deoxynucleotide sequence is C / T; 28. CAEKDK010000008.1_7399902, whose deoxynucleotide sequence is A / G; 29, CAEKDK010000008.1_15579729, whose deoxynucleotide sequence is C / T; The genome accession number of the SNP site reference is GCA_903112645.1, and the apricot is selected from Xinjiang apricot.

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