Debugging Method for the Relative Positions of the PDA and the Exit Slit of a Rear Spectrophotometer

Abstract

The present invention discloses a method for debugging the relative positions of a PDA and an exit slit of a rear spectrophotometer, comprising the following steps: setting the target amplification resistance of a 478nm wavelength channel and the target amplification resistance of a 505nm wavelength channel to be the same; adjusting the spot position of incident light to an effective area of a concave grating diffraction surface; adjusting the position of diffracted light from the concave grating diffraction surface to an effective area of a filter; adding a collection liquid into a cuvette, placing the cuvette at positions specified by front and rear lens assemblies, and performing cyclic collection; and continuously adjusting the position of the PDA until the AD value of the 478nm wavelength channel is half of the AD value of the 505nm wavelength channel, and then stopping. The debugging process takes a short time and reduces the requirements on the operator.

Description

Technical Field

[0001] The present invention relates to the technical field of spectrophotometer operation, and in particular to a method for debugging the relative positions of a PDA and an exit slit of a rear spectrophotometer. Background Art

[0002] The spectrophotometer is one of the main devices used in the optical system of current biochemical analyzers. When using a spectrophotometer, it is necessary to ensure that the array of the photosensitive surface of the PDA accurately corresponds to the wavelength channels of the exit slit. There are two main existing adjustment methods: First, the data is generally tested repeatedly and the relative position of the two is repeatedly adjusted to ensure that the absorbance accuracy of the required wavelength channel meets the standard. However, this method has the disadvantages of being extremely difficult to operate and extremely time-consuming. Second, the PDA and exit slit are placed under a high-power magnifying glass, and the position of the PDA array and the wavelength channels of the exit slit are observed. Gradually adjust to ensure that the wavelength channels on the exit slit correspond to the PDA array, and then glue is applied to fix them. This method is difficult to operate, time-consuming, and requires high operator skills. Moreover, once the exit slit is fixed by glue, it cannot be adjusted again. Summary of the Invention

[0003] The object of the present invention is to provide a method for debugging the relative positions of the PDA and the exit slit of a post-spectrophotometer to solve the problems existing in the two debugging methods mentioned above. The debugging method is simple to operate, has basically no requirements on the operator, and when the target amplifier resistance of the 478nm wavelength channel is the same as the target amplifier resistance of the 505nm wavelength channel, when the AD value of the former is half of the latter, it indicates that the positions of the PDA photosensitive surface array and the exit slit in each wavelength channel are aligned.

[0004] The present invention provides a method for debugging the relative position of a PDA and an exit slit of a post-spectrophotometer, comprising the following steps:

[0005] The target amplification resistance of the 478nm wavelength channel and the target amplification resistance of the 505nm wavelength channel are set to be the same;

[0006] Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0007] Adjusting the position of the diffracted light from the concave grating diffraction surface to the effective area of the filter;

[0008] Add the collection liquid into the cuvette, place it in the specified position of the front and rear lens assemblies, and collect the sample in a cycle;

[0009] The position of the PDA is continuously adjusted until the AD value of the 478 nm wavelength channel is half of the AD value of the 505 nm wavelength channel.

[0010] The beneficial effects of the present invention are as follows: the present invention only requires that the target amplification resistance of the 478nm wavelength channel and the target amplification resistance of the 505nm wavelength channel be the same. During the process of adjusting the position of the PDA, when the AD value of the 478nm wavelength channel is half of the AD value of the 505nm wavelength channel, it can be indicated that the positions of the PDA photosensitive surface array and the wavelength channels of the exit slit have been aligned. Therefore, it can be seen that the debugging method disclosed in the present invention is simple to operate, has low requirements on the operator and takes a short time, and is therefore worthy of popularization and use.

[0011] As a possible implementation, the target amplification resistance is 1 to 5 megohms, which is conducive to the observation and comparison of brightness data of two wavelength channels.

[0012] As a possible preferred embodiment, the target amplification resistance is 1 to 3 megohms, which makes it easier to observe and compare the brightness data of the two wavelength channels.

[0013] As a possible more preferred embodiment, the target amplification resistance is 1 megohm, which makes it easiest to observe and compare the brightness data of the two wavelength channels.

[0014] As a possible implementation manner, the amount of purified water added is one half to two thirds of the volume of the quartz cuvette.

[0015] As a possible implementation method, the calculation formula for light intensity in the process of adjusting the position of the PDA is I = Nhv / At, where: I is the light intensity (unit: cd), N is the total number of photons that illuminate the PDA lighting area within the time interval t (unit: pieces), h is the Planck constant (6.6260693(11)×10^(-34)J·s), v is the frequency of photon irradiation (unit: Hz), A is the PDA lighting area (unit: m2), and t is the time interval (unit: s).

[0016] As a possible implementation, the 478 nm corresponds to the PDA photosensitive surface array 8, and half of the 505 nm corresponds to the PDA photosensitive surface array 9.

[0017] As a possible implementation manner, the intensity of the 478 nm light is half of the intensity of the 505 nm light.

[0018] As a possible implementation manner, the cuvette is a quartz cuvette.

[0019] As a possible implementation, the collection liquid is purified water. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Graph showing the correspondence between wavelength position and PDA lighting area in an embodiment of the present invention. DETAILED DESCRIPTION

[0021] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0022] There are two existing debugging methods for spectrophotometers: Method 1: Generally, data is repeatedly tested and the relative position relationship between the two is repeatedly adjusted to ensure that the absorbance accuracy of the required wavelength channel meets the standard; Method 2: The PDA and the exit slit are placed under a high-power magnifying glass, and the positions of the wavelength channels of the PDA array and the exit slit are observed, and gradually adjusted so that the wavelength channels on the exit slit correspond to the PDA array, and then glue is applied to fix them. The inventors of the present invention found that both of the above debugging methods are difficult to operate, have high requirements for operators, and are time-consuming. In addition, the second debugging method cannot be debugged again once the glue is applied to fix it.

[0023] In response to the above-mentioned problems existing in the two existing debugging methods, the inventors of the present invention accidentally discovered that by selecting the 478nm wavelength channel and the 505nm wavelength channel, adjusting the target amplification resistance of the two to be the same, and only adjusting the position of the PDA, the adjustment of the PDA position can be stopped when the AD value of the 478nm wavelength channel is half of the AD value of the 505nm wavelength channel, and it is indicated that the positions of the PDA photosensitive surface array and the wavelength channels of the exit slit have been aligned; based on the above-mentioned inventive concept, it can be seen that the debugging method is simple to operate and does not require repeated adjustments because during the adjustment process, debugging can be stopped as long as the AD value of the 478nm wavelength channel is half of the AD value of the 505nm wavelength channel. Therefore, the time required for debugging is short and the requirements for the operator are also reduced.

[0024] The present invention discloses a method for debugging the relative position of a PDA and an exit slit of a post-spectrophotometer, comprising the following steps:

[0025] S1. The target amplifier resistance of the 478nm wavelength channel and the target amplifier resistance of the 505nm wavelength channel are set to be the same;

[0026] In the present invention, 478nm and 505nm wavelengths are adjacent wavelengths. Using monochromatic light of the same color, the target amplified resistance is calculated according to the formula between brightness and target amplified resistance: "Target amplified resistance equals target brightness divided by actual brightness multiplied by actual target amplified resistance." In the present invention, the target amplified resistance of the 478nm wavelength channel and the 505nm wavelength channel are set to be the same primarily to facilitate observation and comparison of brightness data for the two wavelength channels. As long as the target amplified resistances are the same for both wavelength channels, the target amplified resistance is generally 1 to 5 megohms, preferably 1 to 3 megohms. Based on actual conditions such as target brightness, a target amplified resistance of 1 megohm is more appropriate.

[0027] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0028] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0029] S4. Add the collection solution to the cuvette, place it in the specified position of the front and rear lens assemblies, and cycle through the collection process;

[0030] In the present invention, the cuvette is generally made of quartz, but is not limited thereto. In the present invention, the collection liquid is generally purified water, but is not limited thereto.

[0031] S5. Continue adjusting the position of the PDA until the AD value of the 478nm wavelength channel is half of the AD value of the 505nm wavelength channel.

[0032] In the present invention, the length, width and height of the 478nm wavelength and the 505nm wavelength are the same, that is, the area is the same. Figure 1 The correspondence between wavelength position and PDA light-collecting area, as well as the light intensity calculation formula I = Nhv / At (I = light intensity, v = frequency, A = illuminated area, N = the total number of photons striking A during time interval t, h = Planck's constant), shows that the entire 478nm wavelength corresponds to PDA photosensitive array 8, and half the 505nm wavelength corresponds to PDA photosensitive array 9. Therefore, when the 478nm light intensity is half the 505nm light intensity, it indicates that the positions of the PDA photosensitive array and the exit slit wavelength channels are aligned. This eliminates the need for microscopic observation after each adjustment.

[0033] In the present invention, the position of the PDA can be adjusted in a horizontal direction.

[0034] The present invention omits the step of observing under a microscope every time an adjustment is made, and during the debugging process, only the position of the PDA needs to be adjusted, while the step of adjusting the position of the exit slit is omitted, thereby making the operation simpler and more controllable.

[0035] Example

[0036] Example 1

[0037] In this embodiment, the debugging method is described by taking the rear spectrophotometer of the XC8001 fully automatic biochemical analyzer of Sichuan Xinjiancheng Biological Co., Ltd. as an example. The debugging method includes the following steps:

[0038] S1. Set the target amplifier resistance of the 478nm wavelength channel and the target amplifier resistance of the 505nm wavelength channel of the same color monochromatic light to 1 megohm;

[0039] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0040] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0041] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0042] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 478 nm wavelength channel is half of the AD value of the 505 nm wavelength channel.

[0043] Place the PDA photosensitive surface array and the exit slit under a magnifying glass to check whether the positions of each wavelength channel are aligned. The result shows that they are aligned.

[0044] The specific test method is to place the rear spectrophotometer's rear spectrophotometer module at the optical signal output of a deuterium / tungsten lamp grating spectrometer. The tungsten lamp signal from the deuterium / tungsten lamp grating spectrometer is then directed through wavelength channels of 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm, observing the AD value. When the AD value reaches a maximum value for each corresponding wavelength channel, it indicates that the PDA photosensitive surface array and the exit slit are aligned for each wavelength channel. The grating spectrometer used in this test is a WDS-3 modular multifunctional grating spectrometer from Tianjin Top Instrument Co., Ltd.

[0045] Example 2

[0046] This embodiment uses the spectrophotometer of Sichuan Xinjiancheng Biological Co., Ltd., model XC8001, a fully automatic biochemical analyzer, as an example to illustrate the debugging method. The debugging method includes the following steps:

[0047] S1. The target amplifier resistance of the 478nm wavelength channel and the target amplifier resistance of the 505nm wavelength channel of the same color monochromatic light are both set to 3 megohms;

[0048] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0049] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0050] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0051] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 478 nm wavelength channel is half of the AD value of the 505 nm wavelength channel.

[0052] Place the PDA photosensitive surface array and the exit slit under a magnifying glass to check whether the positions of each wavelength channel are aligned. The result shows that they are aligned.

[0053] The specific test method is to place the rear spectrophotometer's rear spectrophotometer module at the optical signal output of a deuterium / tungsten lamp grating spectrometer. The tungsten lamp signal from the deuterium / tungsten lamp grating spectrometer is then directed through wavelength channels of 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm, observing the AD value. When the AD value reaches a maximum for each corresponding wavelength channel, it indicates that the PDA photosensitive surface array and the exit slit are aligned for each wavelength channel. The grating spectrometer used in this test is a WDS-3 modular multifunctional grating spectrometer from Tianjin Top Instrument Co., Ltd.

[0054] Example 3

[0055] This embodiment uses the spectrophotometer of the model XC8001 fully automatic biochemical analyzer produced by Sichuan Xinjiancheng Biological Co., Ltd. as an example to illustrate the debugging method. The debugging method includes the following steps:

[0056] S1. The target amplifier resistance of the 478nm wavelength channel and the target amplifier resistance of the 505nm wavelength channel of the same color monochromatic light are both set to 5 megohms;

[0057] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0058] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0059] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0060] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 478 nm wavelength channel is half of the AD value of the 505 nm wavelength channel.

[0061] Place the PDA photosensitive surface array and the exit slit under a magnifying glass to check whether the positions of each wavelength channel are aligned. The result shows that they are aligned.

[0062] The specific detection method is to place the rear spectrophotometer's rear spectrophotometer module at the optical signal output of the deuterium / tungsten lamp grating spectrometer. The tungsten lamp light signal from the deuterium / tungsten lamp grating spectrometer is then directed through wavelength channels of 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm, observing the AD value. When the AD value reaches a maximum value for each corresponding wavelength channel, it indicates that the PDA photosensitive surface array and the exit slit are aligned for each wavelength channel. The grating spectrometer model used here is the WDS-3 modular multifunctional grating spectrometer from Tianjin Top Instrument Co., Ltd.

[0063] Comparative Example 1

[0064] This comparative example takes the spectrophotometer of the model XC8001 fully automatic biochemical analyzer produced by Sichuan Xinjiancheng Biological Co., Ltd. as an example to illustrate the debugging method, and the debugging method includes the following steps:

[0065] S1. Set the target amplifier resistance of the 478nm wavelength channel and the target amplifier resistance of the 505nm wavelength channel of the same color monochromatic light to 1 megohm;

[0066] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0067] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0068] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0069] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 478 nm wavelength channel is one third of the AD value of the 505 nm wavelength channel.

[0070] Place the PDA photosensitive surface array under a magnifying glass to check whether the positions of each wavelength channel of the exit slit are aligned. The result is misalignment. The specific detection method is: place the rear spectrophotometer's rear spectrophotometer module as a whole at the optical signal output end of the deuterium / tungsten light grating spectrometer, set the tungsten light signal of the deuterium / tungsten light grating spectrometer to pass through the 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm wavelength channels in sequence, and observe the AD value. When the light passes through each corresponding wavelength channel, the AD value does not reach the maximum. At this time, it indicates that the positions of each wavelength channel of the PDA photosensitive surface array and the exit slit are misaligned. The grating spectrometer model here is the WDS-3 combined multifunctional grating spectrometer of Tianjin Top Instrument Co., Ltd.

[0071] Comparative Example 2

[0072] This comparative example takes the spectrophotometer of the model XC8001 fully automatic biochemical analyzer produced by Sichuan Xinjiancheng Biological Co., Ltd. as an example to illustrate the debugging method, and the debugging method includes the following steps:

[0073] S1. Set the target amplifier resistance of the 478nm wavelength channel and the target amplifier resistance of the 505nm wavelength channel of the same color monochromatic light to 1 megohm;

[0074] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0075] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0076] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0077] S5. Continue to adjust the position of the PDA in the horizontal direction until the AD value of the 478nm wavelength channel is two-thirds of the AD value of the 505nm wavelength channel.

[0078] Place the PDA photosensitive surface array under a magnifying glass to check whether the positions of each wavelength channel of the exit slit are aligned. The result is misalignment. The specific detection method is: place the rear spectrophotometer's rear spectrophotometer module as a whole at the optical signal output end of the deuterium / tungsten light grating spectrometer, set the tungsten light signal of the deuterium / tungsten light grating spectrometer to pass through the 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm wavelength channels in sequence, and observe the AD value. When the light passes through each corresponding wavelength channel, the AD value does not reach the maximum. At this time, it indicates that the positions of each wavelength channel of the PDA photosensitive surface array and the exit slit are misaligned. The grating spectrometer model here is the WDS-3 combined multifunctional grating spectrometer of Tianjin Top Instrument Co., Ltd.

[0079] Comparative Example 3

[0080] This comparative example takes the spectrophotometer of the model XC8001 fully automatic biochemical analyzer produced by Sichuan Xinjiancheng Biological Co., Ltd. as an example to illustrate the debugging method, and the debugging method includes the following steps:

[0081] S1. The target amplifier resistance of the 478nm wavelength channel and the target amplifier resistance of the 542nm wavelength channel of the same color monochromatic light are both set to 1 megohm;

[0082] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0083] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0084] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0085] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 478 nm wavelength channel is half of the AD value of the 542 nm wavelength channel.

[0086] Place the PDA photosensitive surface array under a magnifying glass to check whether the positions of each wavelength channel of the exit slit are aligned. The result is misalignment. The specific detection method is: place the rear spectrophotometer's rear spectrophotometer module as a whole at the optical signal output end of the deuterium / tungsten light grating spectrometer, set the tungsten light signal of the deuterium / tungsten light grating spectrometer to pass through the 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm wavelength channels in sequence, and observe the AD value. When the light passes through each corresponding wavelength channel, the AD value does not reach the maximum. At this time, it indicates that the positions of each wavelength channel of the PDA photosensitive surface array and the exit slit are misaligned. The grating spectrometer model here is the WDS-3 combined multifunctional grating spectrometer of Tianjin Top Instrument Co., Ltd.

[0087] Comparative Example 4

[0088] This comparative example takes the spectrophotometer of the model XC8001 fully automatic biochemical analyzer produced by Sichuan Xinjiancheng Biological Co., Ltd. as an example to illustrate the debugging method, and the debugging method includes the following steps:

[0089] S1. The target amplifier resistance of the 450nm wavelength channel and the target amplifier resistance of the 505nm wavelength channel of the same color monochromatic light are both set to 1 megohm;

[0090] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0091] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0092] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0093] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 450nm wavelength channel is half of the AD value of the 505nm wavelength channel.

[0094] Place the PDA photosensitive surface array under a magnifying glass to check whether the positions of each wavelength channel of the exit slit are aligned. The result is misalignment. The specific detection method is: place the rear spectrophotometer's rear spectrophotometer module as a whole at the optical signal output end of the deuterium / tungsten light grating spectrometer, set the tungsten light signal of the deuterium / tungsten light grating spectrometer to pass through the 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm wavelength channels in sequence, and observe the AD value. When the light passes through each corresponding wavelength channel, the AD value does not reach the maximum. At this time, it indicates that the positions of each wavelength channel of the PDA photosensitive surface array and the exit slit are misaligned. The grating spectrometer model here is the WDS-3 combined multifunctional grating spectrometer of Tianjin Top Instrument Co., Ltd.

[0095] Comparative Example 5

[0096] This comparative example takes the spectrophotometer of the model XC8001 fully automatic biochemical analyzer produced by Sichuan Xinjiancheng Biological Co., Ltd. as an example to illustrate the debugging method, and the debugging method includes the following steps:

[0097] S1. The target amplifier resistance of the 478nm wavelength channel of the same color monochromatic light is set to 5 megohms, and the target amplifier resistance of the 505nm wavelength channel is set to 1 megohm;

[0098] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0099] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0100] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0101] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 478 nm wavelength channel is half of the AD value of the 505 nm wavelength channel.

[0102] Place the PDA photosensitive surface array and the exit slit under a magnifying glass to check whether the positions of each wavelength channel are aligned. The result is misalignment. The specific detection method is: place the rear spectrophotometer's rear spectrophotometer module as a whole at the optical signal output end of the deuterium / tungsten light grating spectrometer, set the tungsten light signal of the deuterium / tungsten light grating spectrometer to pass through the 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, and 850nm wavelength channels in sequence, and observe the AD value. When the AD value does not reach the maximum when the light passes through each corresponding wavelength channel, it indicates that the PDA photosensitive surface array and the exit slit's positions of each wavelength channel are misaligned. The grating spectrometer model here is the WDS-3 combined multifunctional grating spectrometer of Tianjin Top Instrument Co., Ltd.

[0103] Comparative Example 6

[0104] This comparative example takes the spectrophotometer of the model XC8001 fully automatic biochemical analyzer produced by Sichuan Xinjiancheng Biological Co., Ltd. as an example to illustrate the debugging method, and the debugging method includes the following steps:

[0105] S1. The target amplifier resistance of the 478nm wavelength channel of the same color monochromatic light is set to 2 megohms, and the target amplifier resistance of the 505nm wavelength channel is set to 1 megohm;

[0106] S2. Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface;

[0107] S3. Adjusting the position of the diffracted light of the concave grating diffraction surface to the effective area of the filter;

[0108] S4. Add purified water to the quartz cuvette to fill it to two-thirds of its volume, place it in the specified positions of the front and rear lens assemblies, and cycle through the collection;

[0109] S5. Continue adjusting the position of the PDA in the horizontal direction until the AD value of the 478 nm wavelength channel is half of the AD value of the 505 nm wavelength channel.

[0110] The PDA photosensitive surface array and the positions of each wavelength channel of the exit slit were checked under a magnifying glass to see if they were aligned. The result was that they were not aligned.

[0111] The specific detection method is: place the rear spectrophotometer's rear spectrophotometer module as a whole at the optical signal output end of the deuterium / tungsten lamp grating spectrometer, set the tungsten lamp light signal of the deuterium / tungsten lamp grating spectrometer to pass through 340nm, 405nm, 450nm, 478nm, 505nm, 542nm, 570nm, 605nm, 630nm, 660nm, 700nm, 750nm, 805nm, 850nm wavelength channels in turn, observe the AD value, when the light passes through each corresponding wavelength channel, the AD value does not reach the maximum, at this time, it indicates that the positions of the PDA photosensitive surface array and the exit slit wavelength channels are not aligned. The grating spectrometer model here is the WDS-3 combined multifunctional grating spectrometer of Tianjin Top Instrument Co., Ltd.

[0112] From the above, it can be seen that the positions of the PDA photosensitive surface array and the wavelength channels of the exit slit need to be aligned. First, the two selected wavelength channels must be adjacent, and the two wavelength channels must be a 478nm wavelength channel and a 505nm wavelength channel; secondly, the target amplification resistance of the two wavelength channels must be the same, preferably 1 megohm; finally, the AD value of the 478nm wavelength channel must be half of the AD value of the 505nm wavelength channel.

[0113] First, it is very easy to set the target amplification resistance of the 478nm wavelength channel and the 505nm wavelength channel to be the same, and the operating skills of the operator are not high; secondly, during the entire debugging process, it is only necessary to adjust the position of the PDA, and there is no need to adjust the positions of the wavelength channels of the exit slit, which simplifies the operation process and makes the operation process easier to master; finally, it is only necessary to read the AD values of the two wavelength channels with the naked eye, and when the AD value of the 478nm wavelength channel is half of the AD value of the 505nm wavelength channel, it indicates that the positions of the PDA photosensitive surface array and the wavelength channels of the exit slit have been aligned, so there is no need to check under a microscope every time an adjustment is made. It can be seen that the technical solution disclosed in the present invention is simple to operate, and can obviously save time and reduce the requirements for the operating experience of the operator; therefore, the debugging method disclosed in the present invention is suitable for popularization and use.

[0114] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for adjusting the relative position of a PDA and an exit slit of a post-spectrophotometer, characterized in that: The debugging method comprises the following steps: The target amplification resistance of the 478nm wavelength channel and the target amplification resistance of the 505nm wavelength channel are set to be the same; Adjust the spot position of the incident light to the effective area of the concave grating diffraction surface; Adjusting the position of the diffracted light from the concave grating diffraction surface to the effective area of the filter; Add the collection liquid into the cuvette, place it in the specified position of the front and rear lens assemblies, and collect the sample in a cycle; Continue to adjust the position of the PDA until the AD value of the 478nm wavelength channel is half of the AD value of the 505nm wavelength channel.

2. The method for adjusting the relative position of the PDA and the exit slit of the post-spectrophotometer according to claim 1, characterized in that: The target amplified resistance is 1 to 5 megohms.

3. The method for adjusting the relative position of the PDA and the exit slit of the post-spectrophotometer according to claim 2, characterized in that: The target amplified resistance is 1 to 3 megohms.

4. The method for adjusting the relative position of the PDA and the exit slit of the post-spectrophotometer according to claim 3, characterized in that: The target amplified resistance is 1 megohm.

5. The method for adjusting the relative position of the PDA and the exit slit of the post-spectrophotometer according to claim 1, characterized in that: The collection liquid is purified water, and the amount of the purified water added is one half to two thirds of the volume of the quartz cuvette.

6. The method for adjusting the relative position of the PDA and the exit slit of the post-spectrophotometer according to claim 1, characterized in that: The calculation formula of light intensity in the process of adjusting the position of PDA is I=Nhv / At, Where: I is the light intensity, unit: cd; N is the total number of photons that illuminate the PDA light-collecting area within the time interval t, unit: number; h is Planck's constant, 6.626069311×10^-34 J·s; v is the frequency of photon irradiation, unit: Hz; A is the PDA lighting area, unit: m2; t is the time interval, unit: s.

7. The method for adjusting the relative position of the PDA and the exit slit of the post-spectrophotometer according to claim 6, wherein In, The intensity of the 478 nm light is half of the intensity of the 505 nm light.

8. The method for adjusting the relative position of the PDA and the exit slit of the post-spectrophotometer according to claim 1, characterized in that: The cuvette is a quartz cuvette.

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