Tester capable of automatically adjusting optical density

Abstract

The utility model discloses a tester capable of automatically adjusting optical density and concentration, the overall appearance of the tester is in an elliptic cylinder shape, the tester is provided with a main body shell and an inner wall, the main body shell, an internal first-layer space and an internal second-layer space are sequentially arranged at the upper part from outside to inside, the internal first-layer space is arranged between the main body shell and the inner wall, and the internal second-layer space is arranged between the main body shell and the inner wall. The interior of the inner wall is an internal second-layer space; a central control panel is mounted on the main body shell, an external light source and a monochromator are arranged in a first layer space inside the main body shell, a second layer space inside the main body shell consists of four cuvettes and a liquid pool positioned in the center, and a vortex oscillator and an ultrasonic dispersion system are arranged at the lower part of the inner wall of the main body shell.

Description

Technical Field

[0001] The utility model relates to a biochemical analysis instrument, in particular to an optical density measuring instrument capable of automatically adjusting the concentrations of microorganisms, nucleic acids and proteins. Background Art

[0002] In bioscience research and medical diagnostics, accurate measurement of the concentration of microorganisms, nucleic acids, and proteins is a routine requirement, which requires the use of a densitometer or optical density meter.

[0003] There are four traditional methods for optical density measurement: (1) The transmission method, which measures the intensity of light transmitted from a thin layer spot, is applicable to the visible light region. Its main disadvantage is that the sorbent and the glass plate itself will absorb light, and the uneven thickness of the thin layer will cause an unstable baseline, which will affect the accuracy of the measurement; (2) The reflection method, which measures the intensity of light reflected from a thin layer spot, is applicable to the ultraviolet-visible light region and requires the thin layer surface to be very flat; (3) The transmission-reflection method, which measures the transmitted light and reflected light at the same time and records the sum of the two light signal values. This method is more ideal, with a stable baseline, small measurement error and high sensitivity; (4) If the compound itself has fluorescence or generates a fluorescent compound after appropriate treatment, the fluorescence measurement method can also be used.

[0004] However, these operations usually require manual work, including sample dilution and suspension, which is not only time-consuming but also prone to introducing operational errors. Therefore, it is necessary to develop an automated device to improve the accuracy and repeatability of measurements. Utility Model Content

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of the existing technology and provide an instrument for automatically adjusting optical density concentration. The instrument can automatically inject samples, dilute, suspend and measure the concentration of microorganisms, nucleic acids and proteins; it is convenient for users to reduce manual operations, improve efficiency and accuracy, and is suitable for different types of biomolecule concentration determination. Through precise dilution and suspension, the repeatability and accuracy of the measurement results are ensured.

[0006] In view of this, the technical solution of the present utility model is as follows:

[0007] A measuring instrument for automatically adjusting optical density concentration, the measuring instrument having an overall elliptical cylindrical shape, comprising a main body shell and an inner wall, wherein the upper portion comprises, from the outside to the inside, the main body shell, an internal first layer space, and an internal second layer space, wherein the space between the main body shell and the inner wall is the internal first layer space, and the interior of the inner wall is the internal second layer space;

[0008] The main shell is equipped with a central control panel, which has a monitoring display and a central control system, so that the user can view the sample OD value in real time and make corresponding adjustments and settings according to the sample OD value.

[0009] The first internal space is equipped with an external light source and a monochromator. The external light source emits light, which is received by the monochromator to produce monochromatic light. Its function is to make corresponding settings according to the absorbance characteristics of different samples. Specifically, it produces monochromatic light of different colors according to different samples. The specific setting method is completed through the central control system.

[0010] The second internal space consists of four cuvettes and a liquid pool in the center. The four cuvettes are connected to the liquid pool in the center through a refill tube. The cuvettes are loaded with samples to be tested, and the liquid pool is loaded with blank samples. When the external light source is processed and filtered by the monochromator to form light of a monochromatic wavelength and then irradiated into the liquid pool, the OD value of the blank sample is detected and read by the central control system and set as the blank value. Then the four cuvettes rotate clockwise at a certain speed to ensure that each sample can be accurately detected by its corresponding OD value. The central control system automatically subtracts the blank value from the OD value of each sample and displays the actual OD value of the sample on the monitoring display screen for real-time observation by the user. If the OD value of the sample is too high, the central control system will calculate the volume of refill required according to the corresponding value, and perform quantitative dilution and refill through the refill tube provided with the liquid pool.

[0011] A vortex oscillator and an ultrasonic dispersion system are provided at the lower part of the inner wall. The vortex oscillator and the ultrasonic dispersion system are located at the bottom of the measuring instrument. The upper part is the main shell, the first internal space and the second internal space. Its function is to assist in mixing the sample before testing, so that the sample is more evenly dispersed and the test results are more reliable and accurate.

[0012] In particular, the four cuvettes are independently provided, so that the detection, reading and dilution of the four cuvettes are all independently operated, and the samples do not affect each other.

[0013] Particularly, the external light source is arranged on the inner wall of the main body shell, and the monochromator is arranged in the inner first layer space and corresponds to the external light source.

[0014] In particular, the cuvette and the liquid pool can rotate under the drive of a motor. Specifically, the liquid pool is connected to a motor, and the liquid pool rotates under the drive of the motor. The cuvette is fixed to the liquid pool through a refill tube. Thus, the cuvette can rotate with the liquid pool to ensure that the corresponding OD value of each sample can be accurately detected.

[0015] It can be seen from the above technical solutions that the embodiments of the present invention have the following advantages:

[0016] 1. The utility model can automatically inject, dilute, suspend and measure the concentration of microorganisms, nucleic acids and proteins.

[0017] 2. The utility model will help users reduce manual operations, improve efficiency and accuracy, and is suitable for different types of biomolecule concentration determinations.

[0018] 3. The utility model ensures the repeatability and accuracy of the measurement results through precise dilution and suspension.

[0019] These features and advantages of the present invention will be disclosed in detail in the following specific embodiments and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be further described below with reference to the accompanying drawings:

[0021] Figure 1 This is a schematic diagram of the structure of the present invention.

[0022] Explanation of the accompanying symbols: 1. Main shell; 2. Central control panel; 3. External light source; 4. Monochromator; 5. Cuvette; 6. Liquid pool; 7. Fluid refilling tube; 8. Vortex oscillator and ultrasonic dispersion system, 9. Inner wall. DETAILED DESCRIPTION

[0023] The following is an explanation and description of the technical solutions of the embodiments of the present invention in conjunction with the drawings of the embodiments of the present invention, but the following embodiments are only preferred embodiments of the present invention and are not exhaustive. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0024] The following describes in detail an instrument for automatically adjusting optical density concentration according to an embodiment of the present invention with reference to the accompanying drawings.

[0025] For easier understanding, see Figure 1 As shown, the measuring instrument for automatically adjusting optical density concentration realized by the present invention has an overall shape of an elliptical cylinder, with a main shell 1 and an inner wall 9. The upper part is composed of the main shell 1, the first internal space and the second internal space from the outside to the inside. The space between the main shell 1 and the inner wall 9 is the first internal space, and the interior of the inner wall 9 is the second internal space.

[0026] As shown in the figure, a central control panel 2 is installed on the surface of the main shell 1. The central control panel 2 has a monitoring display screen and a central control system, which allows users to view the sample OD value in real time on the one hand, and to make corresponding adjustments and settings according to the sample OD value on the other hand; usually, the monitoring display screen is an LCD liquid crystal display screen, and the central control system is a control system for controlling the detection and reading of the sample OD value, the operation of the vortex oscillator and the ultrasonic dispersion system, and the rotation of the cuvette and the liquid pool. The control of detecting and reading the sample OD value, the operation of the vortex oscillator and the ultrasonic dispersion system, and the rotation of the cuvette and the liquid pool can be achieved through existing technology and will not be repeated here.

[0027] The first internal space is equipped with an external light source 3 and a monochromator 4. The external light source 3 is mounted on the inner wall of the main housing 1, while the monochromator 4 is located within the first internal space and corresponds to the external light source 3. The external light source 3 emits light, which the monochromator 4 receives and produces monochromatic light. The function of the external light source 3 and the monochromator 4 is to be configured according to the absorbance characteristics of different samples. Specifically, they produce monochromatic light of different colors according to different samples. Under normal circumstances, the specific configuration method can be completed by the central control system.

[0028] The second internal space is composed of four symmetrically distributed cuvettes 5 and a liquid pool 6 located in the center. The four cuvettes 5 are connected to the liquid pool 6 in the center through a refill tube 7; the cuvettes 5 are loaded with samples to be tested, and the liquid pool 6 is loaded with blank samples. When the external light source is processed and filtered by a monochromator to form light of a monochromatic wavelength (such as detecting microbial samples, the external light source emits light with a wavelength of 600nm after being processed by a monochromator), it is emitted into the liquid pool. The OD value of the blank sample is detected and read by the central control system and set as the blank value. Then the four cuvettes 5 rotate clockwise at a certain speed to ensure that each sample can be accurately detected by its corresponding OD value. The central control system automatically subtracts the blank value from the OD value of each sample and presents the actual OD value of the sample on the monitoring display screen for real-time observation by the user. If the OD value of the sample is too high, the central control system will calculate the volume of refill required according to the corresponding value, and perform quantitative dilution and refill through the refill tube provided in the liquid pool.

[0029] A vortex oscillator and an ultrasonic dispersion system 8 are provided at the lower part of the inner wall 9. Normally, the vortex oscillator and the ultrasonic dispersion system 8 are located at the bottom of the measuring instrument, and the upper part is the main shell 1 and the inner wall. Their function is to assist in mixing the sample before testing, so that the sample is more evenly dispersed and the test results are more reliable and accurate.

[0030] The detection, reading and dilution of the four cuvettes 5 are all independently operated, and the samples do not affect each other, so that the detection results can be obtained more accurately.

[0031] Typically, the cuvette 5 and liquid reservoir 6 rotate under the drive of a motor to ensure that each sample's corresponding OD value is accurately detected. Specifically, the liquid reservoir is connected to a motor, which drives the liquid reservoir 6 to rotate. The cuvette 5 is fixed to the liquid reservoir 6 via a refill tube 7. As a result, the cuvette 5 rotates with the liquid reservoir 6, achieving the purpose of clockwise rotation.

[0032] In summary, the present invention can automatically inject, dilute, suspend, and measure the concentrations of microorganisms, nucleic acids, and proteins. It also reduces manual operations for users, improves efficiency and accuracy, and is suitable for measuring the concentrations of different types of biomolecules.

[0033] The utility model ensures the repeatability and accuracy of the measurement results through precise dilution and suspension using a vortex shaker and an ultrasonic dispersion system.

[0034] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. An instrument for automatically adjusting optical density concentration, characterized in that: An optical density measuring instrument that automatically adjusts optical density concentration has an overall elliptical cylindrical shape, comprising a main body shell and an inner wall. The upper portion comprises, from the outside to the inside, the main body shell, an internal first layer space, and an internal second layer space. The space between the main body shell and the inner wall is the internal first layer space, and the interior of the inner wall is the internal second layer space. The main shell is equipped with a central control panel, which has a monitoring display and a central control system, so that the user can view the sample OD value in real time and make corresponding adjustments and settings according to the sample OD value. The first layer of the internal space is equipped with an external light source and a monochromator. The external light source emits light, and the monochromator receives the light source to produce monochromatic light. The second internal space consists of four cuvettes and a liquid pool in the center. The four cuvettes are connected to the liquid pool in the center through refill tubes. The cuvettes are loaded with samples to be tested, and the liquid pool is loaded with blank samples. A vortex oscillator and an ultrasonic dispersion system are provided at the lower part of the inner wall. The vortex oscillator and the ultrasonic dispersion system are located at the bottom of the measuring instrument. The upper part is the main body shell, the internal first layer space and the internal second layer space.

2. The automatic optical density concentration measuring instrument according to claim 1, characterized in that: Four cuvettes are set up independently.

3. The automatic optical density concentration measuring instrument according to claim 1, characterized in that: The external light source is arranged on the inner wall of the main body shell, and the monochromator is arranged in the first layer of the inner space and corresponds to the external light source.

4. The automatic optical density concentration measuring instrument according to claim 1, characterized in that: The liquid pool is connected to a motor, and the liquid pool rotates under the drive of the motor, and the cuvette is fixed on the liquid pool through a liquid replenishing tube.

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