To-be-detected liquid sample manufacturing mechanism applied to pesticide residue detection

Abstract

The utility model relates to the technical field of pesticide residue detection, in particular to a to-be-detected liquid sample manufacturing mechanism applied to pesticide residue detection. The liquid sample access device comprises a first storage module, a second storage module and a liquid sample transfer module, a sample disc of the first storage module is used for placing a sample bottle, a reaction disc is used for placing a cuvette, a first driving assembly drives the sample disc to rotate, and the sample disc rotates to drive the reaction disc and the cuvette to rotate; the second storage module comprises a reagent disc; the liquid sample transfer module comprises a mechanical arm, a liquid adding needle and a second driving assembly; and the mechanical arm is timely driven to drive the liquid adding needle to rotate, so that the liquid adding needle reaches each sample position, each cuvette position and each reagent position, liquid samples are extracted or released, and the preparation of the to-be-detected liquid samples is realized. According to the to-be-detected liquid sample manufacturing mechanism applied to pesticide residue detection, manufacturing of a to-be-detected liquid sample can be achieved, the pesticide residue detection efficiency can be improved, the labor cost is reduced, and the pesticide residue detection cost is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of pesticide residue detection, particularly to a to-be-measured liquid sample preparation mechanism applied to pesticide residue detection. BACKGROUND

[0002] Pesticides are widely used in the prevention and control of crop diseases and insect pests due to their advantages of high efficiency, rapidness, economy and convenience, but also cause pesticide residues on agricultural products to threaten food safety, therefore, it is necessary to detect pesticide residues on agricultural products by pesticide residue detection equipment. At present, most of the pesticide residue detection is carried out by laboratory conventional detection equipment, which cannot automatically add buffer solution, enzyme, reagent and substrate, and professional technicians are needed to manually detect the agricultural products, which is low in intelligent degree and detection efficiency, and large in size and poor in convenience.

[0003] With the development of the industry, some portable pesticide residue detection equipment has also gradually appeared, such as the utility model patent with the patent name of pesticide residue qualitative and quantitative integrated intelligent detection equipment, which discloses a pesticide residue detection equipment. According to the description and drawings, the integrated intelligent detection equipment is provided with a first turntable, a second turntable and a liquid adding module. The first turntable is provided with a sample liquid groove and a reagent groove on the upper end face, and the second turntable is provided with a reaction groove on the upper end face. The liquid adding module is arranged outside the two turntables and between the two turntables. The liquid samples in the sample liquid groove and the reagent groove are transferred to the reaction groove by the liquid adding module, and the pesticide residue detection is carried out after reaction. However, the equipment is provided with two parallel circular turntables, and the liquid adding module is installed between the two circular turntables, which needs to be provided with multiple driving motor assemblies, and has the disadvantages of complex structure, large occupied area and high implementation cost. UTILITY MODEL CONTENT

[0004] In order to overcome the problems in the related art, the utility model provides a to-be-measured liquid sample preparation mechanism applied to pesticide residue detection, which is more reasonable in structure design, smaller in occupied area and volume, and lower in detection cost on the basis of realizing automatic preparation of pesticide residue detection liquid samples.

[0005] In order to achieve the above-mentioned purpose, the utility model provides a to-be-measured liquid sample preparation mechanism applied to pesticide residue detection, which comprises a liquid sample storage and taking device and a conveying device. The liquid sample storage and taking device comprises a first storage module, a second storage module and a liquid sample transfer module.

[0006] The first storage module comprises a sample disc, a reaction disc and a first driving assembly; the sample disc is provided with a plurality of sample sites and a sample adding window, the sample sites are used for placing sample bottles; the reaction disc is installed below the sample disc, the reaction disc is provided with a plurality of cuvette sites, the cuvette sites are used for placing cuvettes, the cuvette sites and the sample adding window are one-to-one corresponding, liquid samples are injected into the cuvettes through the sample adding window; the first driving assembly is arranged below the sample disc and is fixedly connected with the sample disc, the first driving assembly is used for driving the sample disc to rotate, so as to drive the sample bottles on the sample disc to rotate, the sample disc drives the reaction disc below to rotate, so as to drive the cuvettes on the reaction disc to rotate.

[0007] The second storage module comprises a reagent disc, the reagent disc is provided with a plurality of reagent sites, the reagent sites are used for storing enzymes or reagents, and a shaft hole is arranged at the center of the reagent disc.

[0008] The liquid sample transfer module comprises a mechanical arm, a liquid adding needle and a second driving assembly; the liquid adding needle is installed at the first end of the mechanical arm and is used for extracting and releasing liquid samples; the first end of the mechanical arm penetrates through the shaft hole of the reagent disc, so that the liquid adding needle is located above the reagent sites; the second driving assembly is installed below the reagent disc and is connected with the second end of the mechanical arm, the second driving assembly is used for driving the mechanical arm to ascend and descend or rotate, so as to drive the liquid adding needle to ascend and descend or rotate.

[0009] The conveying device is communicated with the liquid adding needle through a pipeline and is used for driving the liquid adding needle to extract or release liquid samples; the first driving assembly drives the sample disc and the reaction disc to rotate, the second driving assembly timely drives the mechanical arm to drive the liquid adding needle to timely ascend and descend, and timely drives the mechanical arm to drive the liquid adding needle to rotate, so that the liquid adding needle reaches each sample site, each cuvette site and each reagent site, and extracts or releases liquid samples.

[0010] In an optional embodiment, the sample disc is a disc, and a plurality of sample sites are annularly distributed on the edge of the disc, and a plurality of sample adding windows are annularly distributed on the inner side of the sample sites; the reagent disc is a crescent, and the reagent disc is arranged side by side with the sample disc, a plurality of reagent sites are annularly distributed on the side of the reagent disc away from the sample disc, and an arc-shaped part recessed towards the center is arranged on the side of the reagent disc close to the sample disc; the outer circumference of the sample disc is matched and installed with the arc-shaped part of the reagent disc, and a gap exists between the two; when the mechanical arm drives the liquid adding needle to rotate on the reagent disc, the liquid adding needle reaches each reagent site to extract reagents; when the mechanical arm drives the liquid adding needle to rotate to a first preset position outside the reagent disc, the sample disc is driven to rotate to drive the sample bottles on the sample sites to move to the lower side of the liquid adding needle in turn, so as to realize liquid sample injection or extraction; when the mechanical arm drives the liquid adding needle to rotate to a second preset position outside the reagent disc, the sample disc is driven to rotate to drive the reaction disc to rotate, so as to drive the cuvettes on the cuvette sites to move to the lower side of the liquid adding needle in turn, so as to realize liquid sample injection or extraction.

[0011] In an optional embodiment, the outer circumference of the sample disc is provided with an abutting table; the first storage module further comprises a sample containing groove and a cuvette containing groove; the sample containing groove is located below the sample disc, and the top of the outer ring side wall of the sample containing groove abuts against the abutting table of the sample disc; the sample containing groove has a first annular space; the opening of the first annular space faces each sample site, and is used for containing and providing a rotating space for a sample bottle; the cuvette containing groove is located below the cuvette disc and on the inner side of the sample containing groove, and has a second annular space; the opening of the second annular space faces each cuvette site, and is used for containing and providing a rotating space for a cuvette; an annular heating sheet is arranged on the side wall of the cuvette containing groove, and is used for maintaining the reaction temperature of the to-be-tested liquid in the cuvette.

[0012] In an optional embodiment, a plurality of detection ports are arranged on the reaction disc along the radial direction of the reaction disc, and each detection port corresponds to each cuvette site in one-to-one correspondence; a first detection window is arranged on the outer wall of the cuvette containing groove, and a second detection window is arranged on the inner wall of the cuvette containing groove, and the first detection window and the second detection window are respectively located on the two sides of the cuvette site; the emitting end of the spectrometer, the cuvette and the receiving end of the spectrometer are sequentially arranged along the radial direction of the reaction disc.

[0013] In an alternative embodiment, the sample disc is provided with a shaft hole in the center; the first driving assembly comprises a first motor, a first synchronous wheel, a second synchronous wheel, a first synchronous belt, a first bearing frame, a first bearing, a second bearing and a first rotating shaft; the first motor is installed below the sample disc; the first synchronous wheel is sleeved on the output shaft of the first motor; the second synchronous wheel is arranged side by side with the first synchronous wheel and is connected in transmission by the first synchronous belt; the first bearing is installed on the first bearing frame and is located above the second synchronous wheel; the second bearing is installed on the first bearing frame and is located below the second synchronous wheel; the first rotating shaft is installed on the first bearing frame through the first bearing and the second bearing, the upper end of the first rotating shaft is fixedly connected with the sample disc in sequence through the shaft hole of the sample disc, the shaft center of the first bearing and the shaft hole of the sample disc, and the lower end of the first rotating shaft is installed in sequence through the shaft center of the second synchronous wheel and the shaft center of the second bearing and is rotated by the second synchronous wheel; the output shaft of the first motor is rotated to rotate the first synchronous wheel, the second synchronous wheel is rotated in synchronization by the first synchronous belt, and the first rotating shaft is rotated by the second synchronous wheel to rotate the sample disc and the reaction disc.

[0014] In an optional embodiment, the second driving assembly further comprises a second motor, a third synchronous wheel, a fourth synchronous wheel, a second synchronous belt, a second bearing frame, a third bearing, a fourth bearing and a shaft sleeve; the mechanical arm comprises a second rotating shaft and a cantilever, the upper end of the second rotating shaft is installed with the liquid adding needle through the cantilever, the outer wall of the second rotating shaft is provided with a vertical sliding groove along the axial direction, the height of the vertical sliding groove is the same as the lifting height of the liquid adding needle; the second motor is installed below the reagent disc; the third synchronous wheel is sleeved on the output shaft of the second motor; the fourth synchronous wheel is arranged side by side with the third synchronous wheel and is drivingly connected through the second synchronous belt; the fourth synchronous wheel is provided with a fastening groove along the radial direction, the fastening groove is provided with a steel ball and an elastic fastener; the third bearing is installed on the second bearing frame and is located above the fourth synchronous wheel; the fourth bearing is installed on the second bearing frame and is located below the fourth synchronous wheel; the second rotating shaft is sleeved with the shaft sleeve, the second rotating shaft and the shaft sleeve are installed on the second bearing frame through the third bearing and the fourth bearing, the upper end of the second rotating shaft and the shaft sleeve sequentially pass through the shaft center of the third bearing, the shaft hole of the reagent disc and extend upward, the lower end of the second rotating shaft sequentially passes through the shaft center of the fourth synchronous wheel and the shaft center of the fourth bearing and is installed, and is driven to rotate by the fourth synchronous wheel; the shaft sleeve is provided with a radial through hole; the elastic fastener pushes the steel ball through the radial through hole and tightly abuts the steel ball in the vertical sliding groove of the second rotating shaft; the output shaft of the second motor rotates to drive the third synchronous wheel to rotate, the fourth synchronous wheel is synchronously driven to rotate through the second synchronous belt, and the fourth synchronous wheel drives the second rotating shaft, the shaft sleeve, the second rotating shaft and the liquid adding needle to rotate.

[0015] In an optional embodiment, the second driving assembly further comprises a lifting unit, the lifting unit comprises a connecting block, a lifting motor, a sliding block and a guide rail; the first connecting hole and the second connecting hole are arranged side by side on the connecting block; the lifting motor is installed above the connecting block, the screw rod of the lifting motor extends downward and passes through the second connecting hole and is fixedly installed with the connecting block; the guide rail is installed in parallel with the screw rod of the lifting motor; one side of the sliding block is slidingly installed with the guide rail, and the other side is fixedly installed with the connecting block; the lower end of the second rotating shaft passes through the first connecting hole and is fixedly installed with the connecting block through the first connecting hole; when the screw rod of the lifting motor moves up and down, the sliding block slides on the guide rail, the connecting block, the second rotating shaft and the liquid adding needle are lifted, and the vertical sliding groove of the second rotating shaft and the steel ball move relatively.

[0016] In an optional embodiment, the reagent disc is further provided with a needle washing position adjacent to the reagent position, for installing a cleaning device.

[0017] In an alternative embodiment, the delivery device comprises a first valve, a buffer zone, a second valve and a plunger pump in sequence; the first valve is communicated with the liquid adding needle through a pipeline; when the liquid sample is extracted, the plunger pump is operated to provide suction force, and the liquid sample is extracted to the buffer zone through the second valve, the first valve and the liquid adding needle; when the liquid sample is injected, the plunger pump is operated to provide thrust, and the liquid sample stored in the buffer zone is released through the second valve, the first valve and the liquid adding needle.

[0018] The application of the measured liquid sample preparation mechanism for pesticide residue detection has the following beneficial effects:

[0019] Compared with the traditional pesticide residue detection scheme, the technical scheme of the embodiment automatically realizes the preparation of the liquid sample, the transfer of the liquid sample, the addition of reagents (reaction reagents, color developing agents, substrates and other reagents) or enzymes, the mixing of the measured liquid, the addition of the substrate and other functional steps through the cooperation of the liquid sample storage and taking device and the delivery device, and the measured liquid sample is no longer prepared by professional operation of professional technicians, and the structure design is reasonable, the occupied area and volume are small, the convenience and environmental adaptability are stronger, and the pesticide residue detection cost is low.

[0020] It should be understood that the foregoing general description and the following detailed description are only exemplary and explanatory, and cannot limit the utility model.

[0021] In order to better understand and implement, the utility model is described in detail below with reference to the drawings. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 The utility model provides a pesticide residue intelligent detection equipment's perspective view for embodiment;

[0023] Figure 2 The utility model provides a pesticide residue intelligent detection equipment's internal structure schematic view for embodiment;

[0024] Figure 3 The utility model provides a sample disc structure schematic view for embodiment;

[0025] Figure 4 The utility model provides a first drive assembly installation schematic view for embodiment;

[0026] Figure 5 The utility model provides a reaction disc installation schematic view for embodiment;

[0027] Figure 6 The utility model provides a first storage module installation schematic view for embodiment;

[0028] Figure 7A structure schematic view of a sample disc provided by the embodiment of the present application is provided.

[0029] Figure 8 A structure schematic view of a reaction disc provided by the embodiment of the present application is provided.

[0030] Figure 9 A structure schematic view of a cuvette containing groove provided by the embodiment of the present application is provided.

[0031] Figure 10 A schematic view of the driving structure of the second rotating shaft provided by the embodiment of the present application is provided.

[0032] Figure 11 A structure schematic view of the second storage module provided by the embodiment of the present application is provided.

[0033] Figure 12 A cross-sectional schematic view of the second storage module and the liquid sample transfer module provided by the embodiment of the present application is provided.

[0034] Figure 13 An installation schematic view of the reagent bottle in-position detection provided by the embodiment of the present application is provided.

[0035] Figure 14 An installation schematic view among the second rotating shaft, the shaft sleeve and the fourth synchronous wheel provided by the embodiment of the present application is provided.

[0036] Figure 15 A working principle schematic view of the conveying device provided by the embodiment of the present application is provided.

[0037] Reference signs:

[0038] 1, box; 11, frame; 12, base; 2, liquid sample access device; 21, first storage module; 211, sample disc; 212, first decorative sticker; 2111, sample site; 2112, sample adding window; 2113, abutting table; 2114, recessed part; 2115, mounting table; 212, reaction disc; 2121, partition; 2122, protrusion; 2123, cuvette site; 2124, detection port; 2125, silica gel sleeve; 213, first driving assembly; 2131, first motor; 2132, first synchronous wheel; 2133, second synchronous wheel; 2134, first synchronous belt; 2135, first rotating shaft; 21351, clamping part; 2136, first bearing; 2137, second bearing; 214, sample containing groove; 215, cuvette containing groove; 22, second storage module; 221, reagent disc; 2211, reagent site; 2212, in-site detection groove; 23, liquid sample transfer module; 231, mechanical arm; 2311, second rotating shaft; 23111, vertical sliding groove; 232, liquid adding needle; 233, second driving assembly; 2331, second motor; 2332, third synchronous wheel; 2333, fourth synchronous wheel; 23331, fastening groove; 2334, second synchronous belt; 2336, third bearing; 2337, fourth bearing; 2338, shaft sleeve; 2335, lifting unit; 23351, connecting block; 23352, lifting motor; 23353, sliding block; 23354, guide rail; 3, detection device; 31, spectrometer; 32, photoelectric sensor; 4, result output device; 5, enzyme storage device; 51, storage box; 52, enzyme storage bottle; 53, semiconductor refrigeration sheet; 54, cooling fin; 55, temperature sensor; 6, cleaning device; 71, first support; 711, first vertical plate; 712, first motor mounting plate; 713, first bearing frame; 714, first detection mounting frame; 715, second detection mounting frame; 72, second support; 721, second vertical plate; 722, second motor mounting plate; 723, second bearing frame; 81, clean water bottle; 82, buffer solution bottle; 83, waste water bottle; 200, sample bottle; 300, cuvette. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0040] Therefore, the following detailed description of the embodiments of the application provided in the accompanying drawings is not intended to limit the scope of the application claimed, but merely represents selected embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of the application.

[0041] It should be noted that similar reference numbers and letters represent similar items in the following drawings, and therefore, once an item is defined in one drawing, it need not be further defined and explained in subsequent drawings.

[0042] The embodiment of the application discloses a to-be-tested liquid sample manufacturing mechanism applied to pesticide residue detection, is suitable for pesticide residue detection in diversified scenes such as agricultural product quality supervision and inspection, health and epidemic prevention, environmental protection, industrial and commercial management and vegetable wholesale market, adopts an automatic mode to realize the steps of liquid sample transfer, reagent injection, liquid sample shaking and mixing, enzyme addition, substrate addition and pesticide residue quantitative detection. The to-be-tested liquid sample manufacturing mechanism applied to pesticide residue detection is suitable for detection of organophosphorus, carbamate and other pesticide residue components.

[0043] The to-be-tested liquid sample manufacturing mechanism applied to pesticide residue detection comprises a main control device (not shown in the figure), a liquid sample storage and access device electrically connected with the main control device and a conveying device.

[0044] In order to improve the transfer convenience, use convenience and environmental adaptability of the to-be-tested liquid sample manufacturing mechanism applied to pesticide residue detection, the to-be-tested liquid sample manufacturing mechanism can be arranged in a box body 1, the box body 1 comprises an upper cover (not shown in the figure), a frame 11 and a base 12, and the three are installed in cooperation from top to bottom to form the box body 1.

[0045] In the embodiment, the to-be-tested liquid sample manufacturing mechanism is an important component of a pesticide residue intelligent detection device, and the pesticide residue intelligent detection device further comprises a detection device and a result output device arranged in the box body.

[0046] The main control device of the liquid sample preparation mechanism is in communication connection with the liquid sample access device 2, the conveying device, the detection device 3 and the result output device 4. The communication connection mode can be wireless communication or wired communication, so as to realize real-time interaction in the pesticide residue detection process. For example, the liquid sample access device 2, the conveying device, the detection device 3 and the result output device 4 can receive the control signal sent by the main control device or feed back the detection signal to the main control device. In the embodiment, the form of the main control device is not limited, which can be a control board specially used for realizing the control of each stage of the pesticide residue detection, or other intelligent devices such as general-purpose computers, smart tablets, smart phones, smart bands, industrial computers and the like, or a cloud server or other virtual computer. In the embodiment, the main control device is a control board arranged in the box 1.

[0047] In combination Figures 2 to 6 The liquid sample access device 2 comprises a first storage module 21, a second storage module 22 and a liquid sample transfer module 23. The first storage module 21 comprises a sample disc 211, a reaction disc 212 and a first driving assembly 213. The sample disc 211 is provided with a plurality of sample sites 2111 and a plurality of sample adding windows 2112. The sample sites 2111 are used for placing sample bottles 200, and specifically, there are 18 sample sites 2111. The reaction disc 212 is installed below and fixed to the sample disc 211 and can be driven to rotate by the sample disc 211. The reaction disc 212 is provided with a plurality of cuvette sites 2123 for placing cuvettes 300. The cuvette sites 2123 and the sample adding windows 2112 are in one-to-one correspondence, and specifically, there are 18 cuvette sites 2123 and 18 sample adding windows 2112. The liquid sample is injected into the cuvette 300 through the sample adding window 2112. The reaction disc 212 is provided with a plurality of cuvettes 300, so that multi-channel synchronous detection can be realized, multi-channel detection data can be obtained, and the detection efficiency and accuracy can be improved.

[0048] In combination Figure 7 , Figure 8 The reaction disc 212 and the sample disc 211 are both provided with screw holes, and the reaction disc 212 and the sample disc 211 can be fixedly installed through screws or fixedly connected through a matching structure. Optionally, the side of the sample disc 211 close to the reaction disc 212 is provided with a plurality of recesses 2114, and the side of the reaction disc 212 close to the sample disc 211 is provided with a plurality of protrusions 2122. The shape of the protrusion 2122 corresponds to the shape of the recess 2114, so that the reaction disc 212 and the sample disc 211 can be matched and stably installed.

[0049] Optionally, as Figure 5 , Figure 8As shown, each pair of cuvette positions 2123 in the reaction disk 212 is separated by a partition 2121, and a protrusion 2122 is provided on the top of each partition 2121; the shape of the recess 2114 and the protrusion 2122 can be triangular, and a silicone sleeve 2125 is fitted on the protrusion 2122 to make the protrusion 2122 and the recess 2114 fit more tightly.

[0050] Combination Figures 2 to 6 The first driving component 213 is located below and fixedly connected to the sample tray 211. The first driving component 213 drives the sample tray 211 to rotate, causing the sample vials 200 on it to rotate as well. This rotates the sample vials 200 to a suitable position, facilitating the addition of buffer solutions, reagents (reaction reagents, chromogenic agents, substrates, etc.), and enzymes. It also facilitates the transfer of the test solution to the cuvette 300. Simultaneously, the rotation of the sample tray 211 also helps to evenly mix the liquid sample in the sample vials 200 at various stages. The rotation of the sample tray 211 also drives the reaction tray 212 below it to rotate, causing the cuvette 300 on the reaction tray 212 to rotate as well. This facilitates the transfer of the liquid sample soaked in the test substance to the cuvette 300, the addition of chromogenic agents or enzymes, or the cleaning of the cuvette 300.

[0051] like Figures 9 to 12 As shown, the second storage module 22 includes a reagent tray 221 with multiple reagent positions 2211 for storing enzymes or reagents. The center of the reagent tray 221 has a shaft hole for mounting a robotic arm 231.

[0052] To ensure secure installation of the reagent bottles, each reagent position 2211 is a circular hole, with a bottle sleeve installed below it. The upper end of the bottle sleeve passes through the circular hole and is secured with screws. The bottle sleeve has a cylindrical receiving cavity for holding the reagent bottle or test tube.

[0053] Optional, such as Figure 12 As shown, in order to realize in-situ detection of reagent bottles, the reagent tray 221 is provided with an in-situ detection slot 2212 facing each reagent position 2211 on the side near the base. A photoelectric sensor 32 is installed in the in-situ detection slot 2212 for in-situ detection of reagent bottles, test tubes or enzyme storage bottles 52 in each reagent position 2211.

[0054] The liquid sample transfer module 23 comprises a mechanical arm 231, a liquid adding needle 232, and a second driving assembly 233; the liquid adding needle 232 is installed at a first end of the mechanical arm 231 and used for extracting a released liquid sample; the first end of the mechanical arm 231 penetrates through a shaft hole of the reagent disc 221, so that the liquid adding needle 232 is located above the reagent site 2211; the second driving assembly 233 is installed below the reagent disc 221 and connected with a second end of the mechanical arm 231, and is used for driving the mechanical arm 231 to ascend or descend or rotate, so as to drive the liquid adding needle 232 to ascend or descend or rotate. Specifically, the mechanical arm 231 drives the liquid adding needle 232 to rotate around the shaft hole of the reagent disc 221 as a center, with a horizontal distance between the shaft hole and the liquid adding needle 232 as a radius, that is, the liquid adding needle 232 moves along a circumference of a preset circle.

[0055] The conveying device is in communication with the liquid adding needle 232 through a pipeline and is used for driving the liquid adding needle 232 to extract or release a liquid sample at each sample site 2111, each cuvette site 2123, and each reagent site 2211.

[0056] The first driving assembly 213 drives the sample disc 211 and the reaction disc 212 to rotate, the second driving assembly 233 timely drives the mechanical arm 231 to drive the liquid adding needle 232 to timely ascend or descend, and timely drives the mechanical arm 231 to drive the liquid adding needle 232 to rotate along a circumference of a preset circle, so that the liquid adding needle 232 reaches each sample site 2111, each cuvette site 2123, and each reagent site 2211, and extracts or releases a liquid sample.

[0057] Compared with a traditional pesticide residue detection scheme, the technical scheme of the embodiment automatically realizes a function step of liquid sample preparation, liquid sample transfer, reagent (reaction reagent, color developing agent, substrate, and other reagents) or enzyme addition, mixing of a to-be-detected liquid, substrate addition, and the like through cooperation of the liquid sample access device and the conveying device, has high intelligentization, has high liquid sample preparation efficiency, no longer depends on professional operation of a professional technician to prepare a to-be-detected liquid sample, has reasonable structural design, has small floor space and small volume, has stronger convenience and environmental adaptability, and has low pesticide residue detection cost.

[0058] The following will be described in detail from each device of a to-be-detected liquid sample preparation mechanism applied to pesticide residue detection.

[0059] In order to save the space of the box, the number of detection channels and the number of reagents to be added are combined to design a structure.

[0060] In the embodiment, Figures 2-8As shown, the sample disc 211 is arranged as a disc which can be driven to rotate, and the edge of the disc is annularly provided with a plurality of sample sites 2111, which can be 18 equidistant sample sites 2111. The sample bottle 200 is placed in the sample site 2111 with the opening upward. On the sample disc 211, the inner side of the sample site 2111 is annularly provided with a plurality of sample adding windows 2112, which can be 18 equidistant sample adding windows 2112. Optionally, the sample disc 211 is further attached with a first decorative sticker 212 toward the upper surface of the upper cover, and the first decorative sticker 212 is marked with the sample site 2111 number and / or the cuvette site 2123 number.

[0061] As shown, Figure 2 , Figure 9 , Figure 12 The reagent disc 221 is crescent-shaped, and the reagent disc 221 is arranged side by side with the sample disc 211, the reagent disc 221 is not rotatable, and the sample disc 211 is rotatable. The side of the reagent disc 221 away from the sample disc 211 is annularly provided with a plurality of reagent sites 2211, and each reagent site 2211 is equidistantly arranged along the arc, so that the liquid adding needle 232 is more easily controlled to be transferred to each reagent site 2211. Optionally, the reagent disc 221 is provided with five reagent sites 2211, one of which is used for enzyme storage. The side of the reagent disc 221 close to the sample disc 211 is provided with an arc-shaped part concave to the center; the circumferential eaves of the sample disc 211 is matched with the arc-shaped part of the reagent disc 221, and there is a small gap between them, so that the sample disc 211 can rotate freely, so that the mechanical arm 231 can rotate the liquid adding needle 232 to each sample bottle 200, cuvette 300, and reagent bottle to suck or release liquid sample. Optionally, the reagent disc 221 is further attached with a second decorative sticker toward the upper surface of the upper cover, and the second decorative sticker is marked with the reagent site 2211 number, the cleaning tank identification, and / or the enzyme storage identification.

[0062] Specifically, when the mechanical arm 231 drives the liquid adding needle 232 to rotate on the reagent disc 221, the liquid adding needle 232 reaches each reagent site 2211 to extract reagents; when the mechanical arm 231 drives the liquid adding needle 232 to rotate to a first preset position (No. 3 sample site in Figure 2 ) outside the reagent disc 221, the sample disc 211 is driven to rotate to drive the sample bottle 200 on the sample site 2111 to move to the lower side of the liquid adding needle 232 in turn, so as to realize liquid sample injection or extraction; when the mechanical arm 231 drives the liquid adding needle 232 to rotate to a second preset position (No. 1 cuvette site in Figure 2 ) outside the reagent disc 221, the sample disc 211 is driven to rotate to drive the reaction disc 212 to rotate to drive the cuvette 300 on the cuvette site 2123 to move to the lower side of the liquid adding needle 232 in turn, so as to realize liquid sample injection or extraction. Each reagent site 2211, the first preset position, and the second preset position are on the circumference of the same preset circle.

[0063] At the same time, the circumferential outer rim of the sample disc 211 is tightly fitted with the arc-shaped part of the reagent disc 221, which can reduce the radius of rotation of the mechanical arm 231, reduce the platform area, and thus reduce the volume of the box 1, save floor space, facilitate carrying and transportation, and reduce the requirements for the detection environment.

[0064] The sample bottle 200 and the cuvette 300 are prone to shaking when they are rotated, and severe shaking may cause interruption of detection. In order to prevent the sample bottle 200 and the cuvette 300 from shaking during rotation and improve the structural stability of the equipment, a corresponding stabilizing structure is needed to accommodate the sample bottle 200 and the cuvette 300. Preferably, the circumferential outer rim of the sample disc 211 is provided with an abutting table 2113. The first storage module 21 further comprises a sample accommodating groove 214 and a cuvette accommodating groove 215. The sample accommodating groove 214 is located below the sample disc 211 and is not rotatable, and the top of the outer ring sidewall thereof abuts against the abutting table 2113 on the circumferential outer rim of the sample disc 211. The sample accommodating groove 214 has a first annular space; the opening of the first annular space faces each sample site 2111, and is used to accommodate and provide rotation space for the sample bottle 200. The cuvette accommodating groove 215 is located below the reaction disc 212 and on the inner side of the sample accommodating groove 214, and is not rotatable, and has a second annular space; the opening of the second annular space faces each cuvette site 2123, and is used to accommodate and provide rotation space for the cuvette 300; a ring-shaped heating sheet is arranged on the outer sidewall of the cuvette accommodating groove 215, which is used to maintain the reaction temperature of the to-be-tested liquid in the cuvette 300 and provide constant-temperature reaction conditions to ensure the activity of the enzyme.

[0065] Optionally, in order to realize spectrophotometric detection, the agricultural residue detection equipment comprises a light source 232 and a spectrophotometer 233. Figure 8 As shown in the figure, the reaction disc 212 is provided with a plurality of detection ports 2124 along the radial direction thereof, and each detection port 2124 corresponds to each cuvette site 2123; a first detection window 2151 is arranged on the outer wall of the cuvette accommodating groove 215, and a second detection window 2152 is arranged on the inner wall thereof, and the first detection window 2151 and the second detection window 2152 are respectively located on the two sides of the cuvette site 2123. During detection, the sample disc 211 needs to be driven to rotate, thereby driving the reaction disc 212 and the cuvette 300 thereon to rotate, so that the cuvette 300 containing the to-be-tested liquid is located on the same straight line as the first detection window 2151 and the second detection window 2152, thereby facilitating spectrophotometric detection.

[0066] From the perspective of agricultural residue detection, as shown in the figure, Figure 6 , Figure 9The outer wall of the cuvette accommodating groove 215 is provided with a third detection window 2153. The spectrometer 31 of the detection device 3 comprises a transmitting end and a receiving end. The transmitting end, the cuvette 300 and the receiving end are sequentially arranged along the radial direction of the reaction disc 212. The transmitting end emits a light signal which irradiates the liquid to be measured in the cuvette 300 through the third detection window 2153 of the cuvette accommodating groove 215 and the detection port 2124 of the reaction disc 212. The receiving end receives the detection signal through the detection port 2124 of the reaction disc 212 and the second detection window of the cuvette accommodating groove 215.

[0067] To realize the in-situ detection of the cuvette 300, the detection device 3 comprises an optoelectronic sensor 32 for in-situ detection of the cuvette 300. The optoelectronic sensor 32 passes through the second detection window and the detection port 2124 of the reaction disc 212 to perform in-situ detection of the cuvette 300.

[0068] To realize the rotation of the sample disc 211 and the reaction disc 212, the following structure is adopted in the embodiment.

[0069] The center of the sample disc 211 is provided with a shaft hole. The first driving assembly 213 comprises a first motor 2131, a first synchronous wheel 2132, a second synchronous wheel 2133, a first synchronous belt 2134, a first bearing bracket 713, a first bearing 2136, a second bearing 2137 and a first rotating shaft 2135.

[0070] The first motor 2131 is installed below the sample disc 211. The first synchronous wheel 2132 is sleeved on the output shaft of the first motor 2131. The second synchronous wheel 2133 is arranged side by side with the first synchronous wheel 2132 and is drivingly connected through the first synchronous belt 2134. The first bearing 2136 is installed on the first bearing bracket 713 and is located above the second synchronous wheel 2133. The second bearing 2137 is installed on the first bearing bracket 713 and is located below the second synchronous wheel 2133.

[0071] The first rotating shaft 2135 is installed on the first bearing bracket 713 through the first bearing 2136 and the second bearing 2137. The upper end of the first rotating shaft 2135 is fixedly connected with the sample disc 211 by sequentially passing through the shaft hole of the sample disc 211 and the shaft center of the first bearing 2136. The lower end of the first rotating shaft 2135 is installed by sequentially passing through the shaft center of the second synchronous wheel 2133 and the shaft center of the second bearing 2137 and is driven to rotate by the second synchronous wheel 2133. Optionally, the shaft hole of the sample disc 211 is a limiting shaft hole. An installation table 2115 is arranged at the limiting shaft hole. The upper end of the first rotating shaft 2135 is provided with a clamping portion 21351 matched with the installation table 2115. The upper end of the first rotating shaft 2135 is provided with a screw hole for installing a cap.

[0072] The output shaft of the first motor 2131 rotates to drive the first synchronous wheel 2132 to rotate, and the first synchronous wheel 2132 drives the second synchronous wheel 2133 to rotate synchronously through the first synchronous belt 2134, and the second synchronous wheel 2133 drives the first rotating shaft 2135 to rotate to drive the sample disc 211 and the reaction disc 212 to rotate.

[0073] In order to realize the rotation of the mechanical arm 231 and the liquid adding needle 232, the embodiment is realized through the following structure.

[0074] The second driving assembly 233 further comprises a second motor 2331, a third synchronous wheel 2332, a fourth synchronous wheel 2333, a second synchronous belt 2334, a second bearing frame 723, a third bearing 2336, a fourth bearing 2337 and a shaft sleeve 2338.

[0075] The mechanical arm 231 comprises a second rotating shaft 2311 and a cantilever, the upper end of the second rotating shaft 2311 is installed with the liquid adding needle 232 through the cantilever, and the outer wall of the second rotating shaft 2311 is provided with a vertical sliding groove 23111 along the axial direction, and the height of the vertical sliding groove 23111 is the same as the lifting height of the liquid adding needle 232. The length of the cantilever is equal to the radius of a preset circle, and the preset circle takes the shaft hole of the reagent disc 221 as the center, and the horizontal distance between the shaft hole and the liquid adding needle 232 is the radius.

[0076] The second motor 2331 is installed below the reagent disc 221 through a second motor mounting plate 722; the third synchronous wheel 2332 is sleeved on the output shaft of the second motor 2331; the fourth synchronous wheel 2333 is arranged side by side with the third synchronous wheel 2332 and is connected in transmission through the second synchronous belt. As shown in Figure 14 The fourth synchronous wheel 2333 is provided with a fastening groove 23331 along the radial direction, and a steel ball 23332 and an elastic fastener (not shown in the figure) are arranged in the fastening groove 23331. The third bearing 2336 is installed on the second bearing frame 723 and located above the fourth synchronous wheel 2333; the fourth bearing 2337 is installed on the second bearing frame 723 and located below the fourth synchronous wheel 2333; the second rotating shaft 2311 is sleeved with the shaft sleeve 2338, and the second rotating shaft 2311 and the shaft sleeve 2338 are installed on the second bearing frame 723 through the third bearing 2336 and the fourth bearing 2337, the upper end of the second rotating shaft 2311 and the shaft sleeve 2338 sequentially pass through the shaft center of the third bearing 2336 and the shaft hole of the reagent disc 221 and extend upward, and the lower end of the second rotating shaft 2311 sequentially passes through the shaft center of the fourth synchronous wheel 2333 and the shaft center of the fourth bearing 2337 and is installed, and is driven to rotate by the fourth synchronous wheel 2333.

[0077] As shown in Figure 14As shown, in order to eliminate the rotation gap between the second rotating shaft 2311 and the shaft sleeve 2338, a radial through hole (blocked by the fourth synchronous wheel 2333) is arranged on the shaft sleeve 2338; the elastic fastener pushes the steel ball 23332 through the radial through hole, and the steel ball 23332 is tightly installed in the vertical sliding groove 23111 of the second rotating shaft 2311, so that the second rotating shaft 2311 can be driven to rotate or lift.

[0078] The output shaft of the second motor 2331 rotates to drive the third synchronous wheel 2332 to rotate, and the fourth synchronous wheel 2333 is driven to rotate synchronously through the second synchronous belt 2334, and the second rotating shaft 2311, the shaft sleeve 2338, the second rotating shaft 2311, and the liquid adding needle 232 are driven to rotate by the fourth synchronous wheel 2333.

[0079] In order to realize the lifting of the mechanical arm 231, as shown in Figure 11 , Figure 12 As shown, the second driving assembly 233 further includes a lifting unit 2335, and the lifting unit 2335 includes a connecting block 23351, a lifting motor 23352, a sliding block 23353, and a guide rail 23354. Therefore, the first connecting hole and the second connecting hole are arranged side by side on the connecting block 23351; the lifting motor 23352 is installed above the connecting block 23351, the screw rod of the lifting motor 23352 extends downward and passes through the second connecting hole, and the connecting block 23351 is fixedly connected with the lifting motor 23352 through the second connecting hole; the guide rail 23354 is installed in parallel with the screw rod of the lifting motor 23352; one side of the sliding block 23353 is slidingly installed with the guide rail 23354, and the other side is fixedly installed with the connecting block 23351; the lower end of the second rotating shaft 2311 passes through the first connecting hole and is installed; when the screw rod of the lifting motor 23352 moves up and down, the sliding block 23353 slides on the guide rail 23354, and the connecting block 23351, the second rotating shaft 2311, and the liquid adding needle 232 are lifted, and the vertical sliding groove and the steel ball of the second rotating shaft 2311 move relatively.

[0080] In order to realize the cleaning of the liquid adding needle 232, as shown in Figure 10As shown, the sample preparation mechanism of the sample to be tested is also provided with a cleaning device 6; the reagent disc 221 is also provided with a needle cleaning position adjacent to the reagent position 2211 and located on the circumference of the preset circle; the cleaning device 6 is installed at the needle cleaning position, and the cleaning device 6 includes a cleaning tank located at the center of the needle cleaning position, and the bottom of the cleaning tank is provided with a water inlet; the water inlet is communicated with the clean water bottle through a pipeline and a conveying device, and the outer periphery of the cleaning tank is provided with a water outlet tank, and the bottom of the water outlet tank is provided with a water outlet, and the water outlet is communicated with the waste water bottle through a pipeline and a conveying device. The liquid adding needle 232 is driven to be transferred above the cleaning tank, and is controlled to be lowered into the cleaning tank, and the clean water in the bottom water inlet washes the liquid adding needle 232, at the same time, the waste water flows out from the bottom water outlet and is transferred to the waste water bottle by the conveying device. In order to facilitate the rotation control of the liquid adding needle 232, the needle cleaning position and each reagent position 2211 are installed along the arc at equal intervals.

[0081] In order to realize the convenient storage of enzyme, the sample preparation mechanism of the sample to be tested is also provided with an enzyme storage device 5. The enzyme storage device 5 includes a storage box 51, an enzyme storage bottle 52, a semiconductor refrigeration piece 53, a heat dissipation fin 54, and a temperature sensor (not shown in the figure); the storage box 51 is arranged below the reagent disc 221, and the bottom of the box body is provided with a temperature measuring hole (not shown in the figure), and one side wall of the storage box 51 is provided with an installation window; the semiconductor refrigeration piece 53 is installed in the installation window; the enzyme storage bottle 52 is placed in the storage box 51 and corresponds to one of the reagent positions 2211 on the reagent disc 221; the heat dissipation fin 54 is installed outside the storage box 51 and adjacent to the semiconductor refrigeration piece 53; the temperature sensor is arranged in the temperature measuring hole and used for detecting the temperature of the semiconductor refrigeration piece 53.

[0082] In order to realize the conveying of the liquid sample, the sample preparation mechanism of the sample to be tested also includes a conveying device for realizing the extraction, transfer and release of clean water, liquid sample, reagent and enzyme.

[0083] The conveying device includes a first valve (No. 3 valve), a buffer zone, a second valve (No. 4 valve) and a plunger pump which are communicated in sequence; the first valve and the second valve are both three-way valves. Among them, the first channel of the first valve is communicated with the liquid adding needle 232 through a pipeline, the second channel thereof is communicated with the first waste liquid motor through a pipeline, and the third channel thereof is communicated with the buffer zone through a pipeline. The first channel of the second valve is communicated with the buffer zone through a pipeline, the second channel thereof is communicated with the plunger pump, and the third channel thereof is communicated with the peristaltic pump through a pipeline. When the liquid sample is extracted, the plunger pump is operated to provide suction, and the liquid sample is extracted to the buffer zone through the second valve, the first valve and the liquid adding needle 232; when the liquid sample is released, the plunger pump is operated to provide a pushing force, and the liquid sample stored in the buffer zone is released through the second valve, the first valve and the liquid adding needle 232. When the liquid sample is extracted and transferred, the liquid adding needle 232 needs to be rotated, and the sample disc 211 and the reaction disc 212 need to be rotated.

[0084] In order to prevent the plunger pump dry grinding from affecting the service life, the plunger pump needs to be primed with water at each start-up. Optionally, the delivery device further comprises a clean water valve and a third valve (valve No. 5), both of which are three-way valves. The first passage of the third valve is in communication with the first passage of the clean water valve through a pipeline, the second passage thereof is in communication with the plunger pump, and the third passage thereof is suspended.

[0085] The water inlet of the plunger pump is in communication with the clean water bottle through a pipeline, the third valve and the clean water valve. When the plunger pump is primed with water, the second passage of the third valve in communication with the plunger pump is opened, the third passage of the clean water valve in communication with the clean water bottle is opened, and the plunger pump is operated to prime with water through the third valve and the clean water valve.

[0086] During the entire pesticide residue detection process, the liquid adding needle 232 needs to be cleaned. Optionally, the delivery device further comprises a clean water motor, a first waste liquid motor, and a waste liquid valve, which is a three-way valve. The first passage of the clean water valve is in communication with the clean water motor, the second passage thereof is in communication with an external clean water source through a pipeline, and the third passage thereof is in communication with the clean water bottle through a pipeline. The first passage of the waste liquid valve is in communication with the first waste liquid motor, the second passage thereof is in communication with an external waste liquid pool, and the third passage thereof is in communication with a waste liquid bottle. The clean water motor is operated to deliver clean water to the liquid adding needle 232 cleaning tank through the clean water valve to flush the liquid adding needle 232; the first waste liquid motor is synchronously operated to transfer the waste liquid to the waste liquid bottle through the waste liquid valve.

[0087] During the preparation of the liquid sample to be tested, the cuvette 300 needs to be cleaned. Optionally, the delivery device further comprises a second waste liquid motor, one end of which is in communication with the second passage of the first valve through a pipeline, and the other end thereof is in communication with the first passage of the waste liquid valve through a pipeline. During the cleaning of the cuvette 300, clean water is transferred to the cuvette 300 through the liquid adding needle 232 for cleaning. Specifically, when the liquid adding needle 232 absorbs clean water, the first passage and the third passage of the clean water valve are controlled to be in communication, the first passage and the second passage of the third valve are controlled to be in communication, the first passage and the second passage of the second valve are controlled to be in communication, and the first passage and the third passage of the first valve are controlled to be in communication to form a water absorption channel. The plunger pump is controlled to operate to absorb clean water to the buffer zone, the liquid adding needle 232 is controlled to be transferred to the cuvette 300, and the plunger pump is again controlled to operate to release the clean water in the buffer zone to the cuvette 300 to clean the cuvette 300. After cleaning, the second waste liquid motor is controlled to operate, the first passage and the second passage of the first valve are controlled to be in communication, and the first passage and the third passage of the waste liquid valve are controlled to be in communication to transfer the waste liquid in the cuvette 300 to the waste liquid bottle.

[0088] When preparing the liquid to be tested, the buffer solution needs to be transferred to the sample bottle 200 first.

[0089] In order to realize the addition of the buffer solution, the conveying device further comprises a peristaltic pump, a buffer solution valve, a fourth valve (No. 1 valve) and a fifth valve (No. 2 valve), and the buffer solution valve, the fourth valve (No. 1 valve) and the fifth valve are all three-way valves.

[0090] The first channel and the third channel of the buffer solution valve are controlled to be communicated, so that the buffer solution valve is communicated with the buffer solution bottle, the first channel and the second channel of the fifth valve are controlled to be communicated, the first channel and the third channel of the fourth valve are controlled to be communicated, the first channel and the third channel of the second valve are controlled to be communicated, and the peristaltic pump is synchronously controlled to operate to draw the buffer solution to the buffer area. When the liquid adding needle 232 is transferred to the sample bottle 200 which needs to add the buffer solution, the plunger pump is controlled to operate to release the buffer solution in the buffer area into the sample bottle 200. Optionally, there may be some air in the buffer area, so that before the buffer solution is transferred to the buffer area, the air in the buffer area needs to be exhausted, at this time, the first channel and the third channel of the second valve are controlled to be communicated, the first channel and the second channel of the fourth valve are controlled to be communicated, the second channel of the fourth valve is communicated with the outside, and the peristaltic pump is controlled to operate, so that the air in the buffer area is exhausted through the second valve, the peristaltic pump and the fourth valve.

[0091] In an optional embodiment, as shown in Figure 1 、 Figure 2 Fig. 1, the first support 71 is used for mounting and supporting the first storage module 21 and the detection device 3, the second support 72 is used for mounting and supporting the second storage module 22 and the liquid sample transfer module 23, and the third support is used for mounting and supporting the clean water bottle 81, the buffer solution bottle 82 and the waste water bottle 83.

[0092] The first support 71 is located in the middle of the base and comprises two first vertical plates 711, a first motor mounting plate 2131, a first bearing bracket 713, a first detection mounting bracket 714 and a second detection mounting bracket 715. The two first vertical plates 711 are vertically mounted on the base side by side, and the first motor mounting plate 2131 is mounted on the two first vertical plates 711 and is supported by the two first vertical plates 711. The first motor mounting plate 2131 is provided with a motor mounting position and a bearing mounting position, the motor mounting position is used for mounting the first motor 2131, and the bearing mounting position is used for mounting a bearing and a rotating shaft. The first motor 2131 is mounted below the first motor mounting plate 2131, and the output shaft thereof is fixedly mounted with the first synchronous wheel 2132 through the motor mounting position. The second synchronous wheel 2133 is mounted above the first motor mounting plate 2131 and corresponds to the bearing mounting position.

[0093] The first bearing frame 713 is fixedly installed with the first motor mounting plate 2131, and the shaft hole on the first bearing frame 713 corresponds to the bearing mounting position. The first bearing frame 713 comprises a first extension frame and a second extension frame arranged in a vertical direction, and the first extension frame and the second extension frame are respectively used for installing the first bearing 2136 and the second bearing 2137.

[0094] The first detection mounting frame 714 is installed on the left side of the first motor mounting plate 2131, and the side wall of the first detection mounting frame 714 has a step which abuts against the edge of the motor mounting frame and is used for screw fixation. The step of the first detection mounting frame 714 abuts against the second extension frame of the first bearing frame 713 in the horizontal direction. Two vertical supports are arranged on the first detection mounting frame 714, one of which supports the bottom of the cuvette accommodating groove 215 and is fixed with the cuvette accommodating groove 215 through screws, and the other supports the bottom of the sample accommodating groove 214 and is fixed with the sample accommodating groove 214 through screws. Optionally, the vertical support for supporting the cuvette accommodating groove 215 is used for installing the spectrometer 31, one side of the vertical support is used for installing the emitting end of the spectrometer 31, and the other side is used for installing the receiving end of the spectrometer 31. The spectrometer 31 is installed corresponding to the first detection window and the second detection window, so that the spectrometer 31 can perform spectrometric detection on the liquid to be detected in the cuvette 300 through the two detection windows.

[0095] The second detection mounting frame 715 is located on the right side of the first motor mounting plate 2131 and is fixed on the first bearing frame 713 through screws, and is arranged in the opposite direction of the first extension frame and the second extension frame. The photoelectric sensor 32 is installed on the second detection mounting frame 715 and faces the second detection window of the cuvette accommodating groove 215, so that the photoelectric sensor 32 can perform in-situ detection on the cuvette 300 through the second detection window. Optionally, the second detection mounting frame 715 is fixedly installed with the sample accommodating groove 214 through screws.

[0096] The second support 72 is installed on the base and is located on the left side of the first support 71, and has a structure similar to that of the first support 71. Specifically, the second support 72 comprises two second vertical plates 721, a second motor mounting plate 722, and a second bearing frame 723. The two second vertical plates 721 are vertically installed side by side on the base, and the second motor mounting plate 722 is installed on the two second vertical plates 721 and is supported by the two second vertical plates 721. The second motor mounting plate 722 is provided with a motor mounting position and a bearing mounting position, the motor mounting position is used for installing the second motor 2331, and the bearing mounting position is used for installing a bearing and a shaft. The second motor 2331 is installed below the second motor mounting plate 722, and the output shaft thereof is fixedly installed with the third synchronous wheel 2332 through the motor mounting position. The third synchronous wheel 2332 is installed above the second motor mounting plate 722 and corresponds to the bearing mounting position.

[0097] Optionally, the guide rail 23354 is vertically installed between the two second vertical plates 721 and supported below the second motor mounting plate 722. When the lifting motor 23352 drives the screw rod and the sliding block 23353 to slide, the sliding block 23353 moves relative to the guide rail 23354.

[0098] The following will be described in detail the steps of preparing the sample liquid for pesticide residue detection.

[0099] The preparation of the sample liquid for pesticide residue detection can include the following steps: S1: cleaning the liquid adding needle; S2: adding buffer solution to the sample bottle containing the test substance through the liquid adding needle; S3: shaking the test substance and the buffer solution in the sample bottle and precipitating for a few minutes; S4: cleaning the sampling needle, then extracting the supernatant in the sample bottle and transferring it to the cuvette; S5: cleaning the liquid adding needle, extracting the enzyme and adding it to the cuvette; S6: cleaning the liquid adding needle, extracting the color developing agent and adding it to the cuvette for a few minutes; S7: cleaning the liquid adding needle and adding the substrate. As can be seen, the steps of rotating the sample disc, the reaction disc and the liquid adding needle during the preparation of the sample liquid and the pesticide residue detection include at least S1 to S7.

[0100] In combination Figure 2 When the liquid adding needle 232 is cleaned, the second motor 2331 is operated to rotate the liquid adding needle 232 to the needle cleaning position through the second shaft 2311, and the cleaning water on the conveying device is used to wash the liquid adding needle 232. After cleaning, the liquid adding needle 232 is transferred to the appropriate position.

[0101] When the buffer solution is added, the buffer solution is first transferred to the buffer area through the conveying device, then the first motor 2131 is controlled to drive the first shaft 2135 to rotate, thereby driving the sample disc 211 to rotate, so that the sample bottle 200 needing to add the buffer solution is close to the reagent disc 221. When the sample bottle 200 needing to add the buffer solution is on the same circumference of the preset circle, the sample disc 211 stops rotating. Then the second motor 2331 is driven to rotate the second shaft 2311 to move the liquid adding needle 232 above the sample bottle 200 needing to add the buffer solution. The buffer solution stored in the buffer area is released through the conveying device and injected into the sample bottle 200 through the liquid adding needle 232. There are a total of 18 sample bottles 200 on the sample disc 211. The sample disc 211 is rotated to make the sample bottles 200 needing to add the buffer solution close to the reagent disc 221 in turn, and the buffer solution is added by the above method. After the buffer solution is added, the first motor 2131 drives the first shaft 2135 to rotate, thereby driving the sample disc 211 to rotate to shake and mix the buffer solution and the test substance in the sample bottle 200, so as to obtain the sample liquid for testing.

[0102] After the sample liquid to be measured is transferred to the cuvette 300, the liquid needle 232 needs to be cleaned before the enzyme is extracted and added. Specifically, the liquid needle 232 is driven to rotate to the needle cleaning position again for cleaning, and then the liquid needle 232 is driven to rotate to the enzyme storage position. The lifting motor 23352 drives the liquid needle 232 to descend into the enzyme storage bottle 52 to extract the enzyme, and then the lifting motor 23352 drives the liquid needle 232 to ascend away from the enzyme storage bottle 52. The second motor 2331 drives the second rotating shaft 2311 to rotate to drive the liquid needle 232 to move to the second preset position, that is, to move above the corresponding cuvette 300. The lifting motor 23352 drives the liquid needle 232 to descend into the cuvette 300 to inject the enzyme. Through the above method, the addition of the color developing agent and the substrate is realized in turn, and finally the detection device 3 is used for spectrophotometric detection.

[0103] After the sample liquid to be measured is transferred to the cuvette 300, the liquid needle 232 needs to be cleaned before the enzyme is extracted and added. Specifically, the liquid needle 232 is driven to rotate to the needle cleaning position again for cleaning, and then the liquid needle 232 is driven to rotate to the enzyme storage position. The lifting motor 23352 drives the liquid needle 232 to descend into the enzyme storage bottle 52 to extract the enzyme, and then the lifting motor 23352 drives the liquid needle 232 to ascend away from the enzyme storage bottle 52. The second motor 2331 drives the second rotating shaft 2311 to rotate to drive the liquid needle 232 to move to the second preset position, that is, to move above the corresponding cuvette 300. The lifting motor 23352 drives the liquid needle 232 to descend into the cuvette 300 to inject the enzyme. Through the above method, the addition of the color developing agent and the substrate is realized in turn, and finally the detection device 3 is used for spectrophotometric detection.

[0104] The application of the liquid sample to be measured in the pesticide residue detection sample preparation mechanism has the following beneficial effects:

[0105] In the technical scheme of the utility model embodiment, the liquid sample preparation, the liquid sample transfer, the reagent (reaction reagent, color developing agent, substrate and the like) or enzyme addition, the mixing of the liquid to be measured, the addition of the substrate and the like are automatically realized through the cooperation of the liquid sample storage and taking device and the conveying device. The professional operation of the professional technicians is no longer needed to prepare the liquid sample to be measured. The driving motor assembly is less, the structure design is reasonable, the land occupation area and the volume are small, the convenience and the environmental adaptability are stronger, and the pesticide residue detection cost is low.

[0106] Compared with the structure design of two rotating discs and a rotating liquid adding needle in the prior art, the technical scheme of the present application only needs to adopt two driving motors to drive rotation, the control mode is relatively simple, and the structure is more reasonable. The ingenious installation of the sample disc, the reaction disc, the reagent disc and the mechanical arm in structure of the present application makes it possible to realize the transfer of the sample, the addition of the reagent at each stage and the transfer of the liquid sample in the whole liquid sample preparation process through the same liquid adding needle, and only needs to control the sample disc, the reaction disc and the mechanical arm to rotate to realize multiple functions, the control mode is simplified, the floor area of the whole device can be reduced, the box volume is reduced, and the convenience and adaptability of the liquid sample to be detected for pesticide residue detection are improved.

[0107] It should be noted that the above are only the preferred embodiments of the present application and the technical principles applied. Those skilled in the art will understand that the present application is not limited to the specific embodiments described herein, and those skilled in the art can make various obvious changes, re-adjustments and substitutions without departing from the protection scope of the present application. Therefore, although the present application has been described in more detail through the above embodiments, the present application is not limited to the above embodiments, and more other equivalent embodiments can be included without departing from the concept of the present application, and the scope of the present application is determined by the scope of the appended claims.

Claims

1. A sample preparation mechanism for pesticide residue detection, characterized in that, The application relates to a liquid sample accessing device. The liquid sample accessing device comprises a first storage module, a second storage module and a liquid sample transferring module. The first storage module comprises a sample disc, a reaction disc and a first driving assembly; the sample disc is provided with a plurality of sample sites and sample adding windows; the sample sites are used for placing sample bottles; the reaction disc is installed below the sample disc; the reaction disc is provided with a plurality of cuvette sites; the cuvette sites are used for placing cuvettes; the cuvette sites and the sample adding windows are in one-to-one correspondence; liquid samples are injected into the cuvettes through the sample adding windows; the first driving assembly is arranged below the sample disc and is fixedly connected with the sample disc; the first driving assembly is used for driving the sample disc to rotate, so as to drive the sample bottles on the sample disc to rotate; the sample disc drives the reaction disc below to rotate, so as to drive the cuvettes on the reaction disc to rotate; The second storage module comprises a reagent disc; the reagent disc is provided with a plurality of reagent sites; the reagent sites are used for storing enzymes or reagents; the center of the reagent disc is provided with a shaft hole; The liquid sample transferring module comprises a mechanical arm, a liquid adding needle and a second driving assembly; the liquid adding needle is installed at the first end of the mechanical arm and is used for extracting and releasing liquid samples; the first end of the mechanical arm penetrates through the shaft hole of the reagent disc, so that the liquid adding needle is located above the reagent sites; the second driving assembly is installed below the reagent disc and is connected with the second end of the mechanical arm; the second driving assembly is used for driving the mechanical arm to ascend and descend or rotate, so as to drive the liquid adding needle to ascend and descend or rotate; The first driving assembly drives the sample disc and the reaction disc to rotate; the second driving assembly timely drives the mechanical arm to drive the liquid adding needle to ascend and descend and timely drives the mechanical arm to drive the liquid adding needle to rotate, so that the liquid adding needle reaches each sample site, each cuvette site and each reagent site, extracts or releases liquid samples, and realizes the preparation of liquid samples to be measured.

2. The sample preparation mechanism for pesticide residue detection according to claim 1, wherein, The sample disc is a disc; a plurality of sample sites are annularly distributed on the edge of the disc; a plurality of sample adding windows are annularly distributed on the inner side of the sample sites; The reagent disc is crescent-shaped; the reagent disc is arranged side by side with the sample disc; a plurality of reagent sites are annularly distributed on the side of the reagent disc away from the sample disc; an arc-shaped part is arranged on the side of the reagent disc close to the sample disc and is recessed towards the center of the reagent disc; The circumferential eaves of the sample disc is matched with the arc-shaped part of the reagent disc and a gap exists between the circumferential eaves of the sample disc and the arc-shaped part of the reagent disc; When the mechanical arm drives the liquid adding needle to rotate on the reagent disc, the liquid adding needle reaches each reagent site to extract reagents; When the mechanical arm drives the liquid adding needle to rotate to a first preset position outside the reagent disc, the sample disc is driven to rotate, so as to drive the sample bottles on the sample sites to move to the lower side of the liquid adding needle in sequence, and liquid sample injection or extraction is realized; When the mechanical arm drives the liquid adding needle to rotate to a second preset position outside the reagent disc, the sample disc is driven to rotate, so as to drive the reaction disc to rotate, drive the cuvettes on the cuvette sites to move to the lower side of the liquid adding needle in sequence, and liquid sample injection or extraction is realized.

3. The sample preparation mechanism for pesticide residue detection according to claim 2, wherein, The circumferential outer edge of the sample disc is provided with an abutting table; The first storage module further comprises a sample containing groove and a cuvette containing groove; The sample containing groove is located below the sample disc, and the top of the outer ring sidewall thereof abuts against the abutting table of the sample disc; The sample containing groove has a first annular space; the opening of the first annular space faces each sample site, and is used for containing and providing a rotating space for a sample bottle; The cuvette containing groove is located below the cuvette disc and inside the sample containing groove, and has a second annular space; the opening of the second annular space faces each cuvette site, and is used for containing and providing a rotating space for a cuvette; a ring-shaped heating sheet is arranged on the sidewall of the cuvette containing groove, and is used for maintaining the reaction temperature of the to-be-tested liquid in the cuvette.

4. The sample preparation mechanism for pesticide residue detection according to claim 3, wherein, The reaction disc is provided with a plurality of detection ports along the radial direction thereof, and each detection port corresponds to each cuvette site one by one; The outer wall of the cuvette containing groove is provided with a first detection window, and the inner wall thereof is provided with a second detection window; the first detection window and the second detection window are located on the two sides of the cuvette site respectively; The emitting end of the spectrometer, the cuvette and the receiving end of the spectrometer are sequentially arranged along the radial direction of the reaction disc.

5. The sample preparation mechanism for pesticide residue detection according to claim 2, wherein, The center of the sample disc is provided with a shaft hole; The first driving assembly comprises a first motor, a first synchronous wheel, a second synchronous wheel, a first synchronous belt, a first bearing bracket, a first bearing, a second bearing and a first rotating shaft; The first motor is installed below the sample disc; the first synchronous wheel is sleeved on the output shaft of the first motor; the second synchronous wheel is arranged side by side with the first synchronous wheel and is drivingly connected through the first synchronous belt; The first bearing is installed on the first bearing bracket and located above the second synchronous wheel; the second bearing is installed on the first bearing bracket and located below the second synchronous wheel; The first rotating shaft is installed on the first bearing bracket through the first bearing and the second bearing; the upper end of the first rotating shaft is sequentially connected with the shaft hole of the sample disc through the shaft center of the first bearing and the shaft hole of the sample disc, and the lower end of the first rotating shaft is sequentially installed through the shaft center of the second synchronous wheel and the shaft center of the second bearing, and is driven to rotate by the second synchronous wheel; The output shaft of the first motor rotates to drive the first synchronous wheel to rotate, and the second synchronous wheel is synchronously driven to rotate through the first synchronous belt, and the first rotating shaft is driven to rotate by the second synchronous wheel, so as to drive the sample disc and the reaction disc to rotate.

6. The sample preparation mechanism for pesticide residue detection according to claim 2, wherein, The second driving assembly further comprises a second motor, a third synchronous wheel, a fourth synchronous wheel, a second synchronous belt, a second bearing bracket, a third bearing, a fourth bearing and a shaft sleeve; The mechanical arm comprises a second rotating shaft and a cantilever; the second rotating shaft is provided with a vertical sliding groove on the outer wall thereof along the axial direction, and the height of the vertical sliding groove is the same as the lifting height of the liquid adding needle; The second motor is installed below the reagent disc; the third synchronous wheel is sleeved on the output shaft of the second motor; the fourth synchronous wheel is arranged side by side with the third synchronous wheel and is connected in transmission through the second synchronous belt; the fourth synchronous wheel is provided with a fastening groove along the radial direction thereof, and a steel ball and an elastic fastener are arranged in the fastening groove; The third bearing is installed on the second bearing frame and is located above the fourth synchronous wheel; the fourth bearing is installed on the second bearing frame and is located below the fourth synchronous wheel; The second rotating shaft sleeve is provided with the sleeve, and the second rotating shaft and the sleeve are installed on the second bearing frame through the third bearing and the fourth bearing; the upper end of the second rotating shaft and the sleeve successively pass through the shaft center of the third bearing, the shaft hole of the reagent disc and extend upwards; the lower end of the second rotating shaft successively passes through the shaft center of the fourth synchronous wheel and the shaft center of the fourth bearing and is installed, and is driven to rotate by the fourth synchronous wheel; The sleeve is provided with a radial through hole; the elastic fastener pushes the steel ball through the radial through hole and abuts the steel ball against the vertical sliding groove of the second rotating shaft; The output shaft of the second motor rotates, drives the third synchronous wheel to rotate, drives the fourth synchronous wheel to rotate in synchronization through the second synchronous belt, and drives the second rotating shaft, the sleeve, the second rotating shaft and the liquid adding needle to rotate.

7. The sample preparation mechanism for pesticide residue detection according to claim 6, wherein, The second driving assembly further comprises a lifting unit, and the lifting unit comprises a connecting block, a lifting motor, a sliding block and a guide rail; The first connecting hole and the second connecting hole are arranged side by side on the connecting block; The lifting motor is installed above the connecting block, the screw rod of the lifting motor extends downwards and passes through the second connecting hole and is fixedly installed with the connecting block; The guide rail is installed in parallel with the screw rod of the lifting motor; one side of the sliding block is slidably installed with the guide rail, and the other side is fixedly installed with the connecting block; The lower end of the second rotating shaft passes through the first connecting hole and is fixedly installed with the connecting block through the first connecting hole; When the screw rod of the lifting motor moves up and down, the sliding block slides on the guide rail, and the connecting block, the second rotating shaft and the liquid adding needle are lifted and lowered, and the vertical sliding groove of the second rotating shaft and the steel ball move relatively.

8. The sample preparation mechanism for pesticide residue detection according to claim 1, wherein, The reagent disc is further provided with a needle washing position adjacent to the reagent position and used for installing a cleaning device.

9. The sample preparation mechanism for pesticide residue detection according to claim 1, wherein, Further comprising a conveying device, The conveying device comprises a first valve, a buffer zone, a second valve and a plunger pump which are sequentially communicated; the first valve is communicated with the liquid adding needle through a pipeline; When the liquid sample is extracted, the plunger pump operates to provide suction, and the liquid sample is extracted to the buffer zone through the second valve, the first valve and the liquid adding needle; When the liquid sample is injected, the plunger pump operates to provide a pushing force, and the liquid sample stored in the buffer zone is released through the second valve, the first valve and the liquid adding needle.

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