Microfluidic detection chip

Abstract

The application discloses a microfluidic detection chip, which comprises a lysis unit, a buffer unit, a detection unit and a connecting unit. The connecting unit can be simultaneously inserted into a lysis sample outlet and a buffer sample inlet, so that the lysis sample outlet and the second opening are communicated, and the buffer sample inlet and the first opening are communicated. The connecting unit can be simultaneously inserted into a buffer sample outlet and a detection sample inlet, so that the buffer sample outlet and the second opening are communicated, and the detection sample inlet and the first opening are communicated. In the application, each unit is independent and in a closed state, which can prevent internal reagents from being contaminated and affecting the detection accuracy. The lysis unit, the buffer unit and the detection unit are communicated through the connecting unit, so that each detection step is connected with each other. Therefore, the microfluidic chip integrates multiple reaction steps, and it is not necessary to repeatedly place samples from the outside when each step is performed, so that the operation is more convenient, and the detection efficiency is high.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microfluidic devices, and particularly relates to a microfluidic detection chip. BACKGROUND

[0002] The microfluidic chip is a chip operation platform, which can integrate the functions of sampling, dilution, reagent addition, reaction and separation, and can be applied to the research of biological medicine and biosensors and the detection of viruses. In related technologies, the microfluidic chip mostly adopts one-step detection method, directly drives the sample to the detection area, or simultaneously injects the detection reagent and the sample into the chip, and cannot integrate the sample pretreatment operation in the chip, resulting in an incoherent sample detection process, complicated operation and low detection efficiency. SUMMARY

[0003] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a microfluidic detection chip, which can integrate multi-step operations on the sample, is convenient to operate and has high detection efficiency.

[0004] According to the microfluidic detection chip in the embodiment of the present application, the microfluidic detection chip comprises:

[0005] The lysis unit has a lysis cavity, a lysis sample inlet and a lysis sample outlet, the lysis cavity, the lysis sample inlet and the lysis sample outlet are sealed in the interior of the lysis unit, the lysis sample outlet and the lysis sample inlet are in communication with the lysis cavity, and the lysis cavity is used for accommodating a lysis reagent;

[0006] The buffer unit has a buffer cavity, a buffer sample inlet and a buffer sample outlet, the buffer cavity, the buffer sample inlet and the buffer sample outlet are sealed in the interior of the buffer unit, the buffer sample inlet and the buffer sample outlet are in communication with the buffer cavity, and the buffer cavity is used for accommodating a buffer reagent;

[0007] The detection unit has a detection cavity and a detection sample inlet, the detection cavity and the detection sample inlet are sealed in the interior of the detection unit, the detection cavity is in communication with the detection sample inlet, and the detection cavity is used for accommodating a detection reagent;

[0008] The connecting unit has an inner cavity, the inner cavity forms a first opening at one end of the connecting unit, a second opening is formed in the side of the connecting unit, and the first opening and the second opening are in communication with the inner cavity;

[0009] The connecting unit is provided with a plurality of connecting units, one of which can be inserted into the lysate outlet and the buffer sample inlet at the same time to communicate the lysate outlet with the second opening and the buffer sample inlet with the first opening; one of the connecting units can be inserted into the buffer outlet and the detection sample inlet at the same time to communicate the buffer outlet with the second opening and the detection sample inlet with the first opening.

[0010] The microfluidic detection chip provided in the embodiment of the present application has at least the following beneficial effects:

[0011] The microfluidic detection chip provided in the embodiment of the present application has at least the following beneficial effects:

[0012] According to some embodiments of the present application, the lysing cavity, the buffer cavity and the detection cavity are all provided as vacuum cavities.

[0013] According to some embodiments of the present application, the buffer unit further has a plurality of buffer channels, one end of the plurality of buffer channels being in communication with the buffer sample inlet, and the other end of the plurality of buffer channels being in communication with different positions of the buffer cavity.

[0014] According to some embodiments of the present application, the lengths of the plurality of buffer channels are equal, and the communication positions of the buffer channels and the buffer cavity are symmetrically distributed relative to the center line of the buffer cavity.

[0015] According to some embodiments of the present application, the buffer unit further has a mixing channel, two ends of the mixing channel being in communication with the buffer cavity and the buffer outlet, respectively.

[0016] According to some embodiments of the present application, the detection unit has a plurality of detection cavities and a plurality of detection channels, two ends of each detection channel being in communication with the detection sample inlet and the corresponding detection cavity.

[0017] According to some embodiments of the present application, the detection unit has a waste liquid cavity, the waste liquid cavity being in communication with the detection cavity.

[0018] According to some embodiments of the present application, one end of the connecting unit has a sharp end, and the first opening is located at the sharp end.

[0019] According to some embodiments of the present application, a plurality of sealing units are further included, and each of the sealing units is arranged at the cracking sample inlet, the buffer sample inlet, and the detection sample inlet.

[0020] According to some embodiments of the present application, a heating unit is further included, and at least one of the cracking unit, the detection unit, and the detection unit is connected to the heating unit.

[0021] Additional aspects and advantages of the present application will be given in part in the following description, become apparent from the following description, or be learned by practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0022] The present application will be further described below in conjunction with the accompanying drawings and embodiments, in which:

[0023] Figure 1 Structure diagram of an embodiment of the microfluidic chip of the present application;

[0024] Figure 2 Structure diagram of the first state of the microfluidic chip;

[0025] Figure 3 Structure diagram of the second state of the microfluidic chip;

[0026] Figure 4 Structure diagram of the third state of the microfluidic chip;

[0027] Figure 5 Structure diagram of the fourth state of the microfluidic chip;

[0028] Figure 6 Structure diagram of the Figure 1 Structure diagram of an embodiment of the connecting unit in the present application;

[0029] Figure 7 Structure diagram of the Figure 1 Structure diagram of an embodiment of the cracking unit in the present application;

[0030] Figure 8 Structure diagram of the Figure 1 Structure diagram of an embodiment of the partial buffer unit in the present application;

[0031] Figure 9 Structure diagram of the Figure 1 Structure diagram of an embodiment of the partial buffer unit in the present application;

[0032] Figure 10 Structure diagram of the Figure 1 Structure diagram of an embodiment of the detection unit in the present application;

[0033] Figure 11 Structure diagram of the microfluidic chip in an experimental example;

[0034] Figure 12 This is a result image of a detection example for a microfluidic chip.

[0035] Reference numerals: 100, 110, 120, 130, 140, 150, 160, 170; 200, 210, 220, 230, 240, 250, 260, 270, 280, 290; 200, 310, 320, 330, 340, 350, 360, 370, 380, 290; 300, 310, 320, 330, 340, 350, 360, 370; 400, 410, 420, 430; 500, 600; 700, 400; 500, 600; 700, 700. Detailed Implementation

[0036] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0037] In the description of this invention, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0038] In the description of this invention, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0039] In the description of this invention, unless otherwise explicitly defined, terms such as "set up," "install," and "connect" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this invention in conjunction with the specific content of the technical solution.

[0040] In the description of the application, the description of the terms "one embodiment", "some embodiments", "exemplary embodiment", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the application. In the description, the exemplary description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0041] With reference to Figure 1 With Figure 2 , an embodiment of the application provides a microfluidic detection chip, comprising a lysis unit 100, a buffer unit 200 and a detection unit 300, the lysis unit 100, the buffer unit 200 and the detection unit 300 are respectively used for pretreatment, buffer mixing and detection of the sample to be detected, so as to realize the multi-step operation of the microfluidic chip on the sample. Specifically, the lysis unit 100 has a lysis cavity 110, a lysis sample inlet 120 and a lysis sample outlet 130, the lysis cavity 110, the lysis sample inlet 120 and the lysis sample outlet 130 are sealed in the inside of the lysis unit 100, the lysis sample inlet 120 and the lysis sample outlet 130 are both in communication with the lysis cavity 110, the lysis cavity 110 or the lysis sample inlet 120 is preloaded with a lysis reagent 140, and the sample to be detected can be injected from the lysis sample inlet 120 and enter the lysis cavity 110 for lysis; the buffer unit 200 has a buffer cavity 210, a buffer sample inlet 220 and a buffer sample outlet 230, the buffer cavity 210, the buffer sample inlet 220 and the buffer sample outlet 230 are sealed in the inside of the buffer unit 200, the buffer sample inlet 220 and the buffer sample outlet 230 are both in communication with the buffer cavity 210, the buffer cavity 210 is preloaded with a buffer reagent 240, the lysis sample enters the buffer cavity 210, mixes with the buffer reagent 240 and realizes amplification buffer, and the buffer reagent 240 provides suitable pH, reaction enzyme and reaction environment for the detection of the sample; the detection unit 300 has a detection cavity 310 and a detection sample inlet 320, the detection cavity 310 and the detection sample inlet 320 are sealed in the inside of the detection unit 300, the detection cavity 310 and the detection sample inlet 320 are in communication, and the detection cavity 310 is preloaded with a detection reagent 330, the sample enters the detection cavity 310, reacts with the detection reagent 330, and generates a fluorescent signal to indicate the detection result.

[0042] It should be noted that the above-mentioned cracking unit 100, buffer unit 200 and detection unit 300 are independent of each other before the detection operation is performed, and the internal cavity is isolated from the outside world to achieve the sealing effect of the microfluidic chip, avoid the internal reagent from being contaminated, and affect the detection precision. The microfluidic chip further comprises a connecting unit 400, the connecting unit 400 has an inner cavity, the inner cavity forms a first opening 410 at one end of the connecting unit 400, and a second opening 420 is arranged on the side of the connecting unit 400. The first opening 410 and the second opening 420 are in communication with the inner cavity; the connecting unit 400 can be provided with a plurality of connecting units 400, and during the detection process, the sample to be detected is injected into the cracking sample inlet 120 and enters the cracking cavity 110 for cracking. After the cracking is completed, one of the connecting units 400 is inserted into the cracking sample outlet 130 and the buffer sample inlet 220 at the same time, the second opening 420 is in communication with the cracking sample outlet 130, and the first opening 410 is in communication with the buffer sample inlet 220. The sample in the cracking cavity 110 flows through the cracking sample outlet 130, the second opening 420, the inner cavity, the first opening 410, the buffer sample inlet 220 in sequence, and then enters the buffer cavity 210 for amplification and buffering; after the sample buffering is completed, one of the connecting units 400 is inserted into the buffer sample outlet 230 and the detection sample inlet 320 at the same time, so that the second opening 420 is in communication with the buffer sample outlet 230, and the first opening 410 is in communication with the detection sample inlet 320. The sample flows through the second opening 420, the inner cavity, the first opening 410, the detection sample inlet 320 in sequence, and then enters the detection cavity 310 for detection, thereby completing the pretreatment, buffer mixing and detection of the sample.

[0043] Therefore, the microfluidic detection chip provided in the embodiment of the present application is independent and in a closed state between each unit, which can prevent the internal reagent from being contaminated and affect the detection precision. The cracking unit 100, the buffer unit 200 and the detection unit 300 are connected by the connecting unit 400, so that each detection step is connected to each other, and therefore the microfluidic chip integrates multiple reaction steps, and the sample does not need to be placed from the outside when each step is performed, which is convenient to operate and has high detection efficiency.

[0044] It should be noted that the distance between the first opening 410 and the second opening 420 in the connecting unit 400 matches the distance between the sample inlet and the sample outlet to be connected, so as to ensure the connection of the connecting unit 400 to the sample inlet and the sample outlet. Based on this, the distance between the sample inlet and the sample outlet in the two units to be connected by the connecting unit 400 is equal, so as to improve the versatility of the connecting unit 400; for example, the cracking unit 100, the buffer unit 200 and the detection unit 300 can be provided with the same thickness, and the sample inlet and the sample outlet are arranged at the center of the thickness direction of the corresponding unit, so that the connecting unit 400 can be applied to the connection between different units.

[0045] Reference Figure 6The one end of the connecting unit 400 has a pointed end 430, the pointed end 430 of the connecting unit 400 can be pierced into the lysis unit 100, the buffer unit 200 or the detection unit 300, the operation is convenient, the first opening 410 is located at the pointed end 430, after the pointed end 430 is pierced into a certain unit, the first opening 410 directly communicates with the internal cavity of the unit, and the flow path of the sample is formed. The one end of the connecting unit 400 away from the pointed end 430 is closed, the internal cavity of the connecting unit 400 can only communicate with the internal cavities of the lysis unit 100, the buffer unit 200 and the detection unit 300 through the first opening 410 and the second opening 420, and is isolated from the external environment, so that the sealing property of the micro-fluidic detection chip in the detection process is ensured, and the detection precision is improved.

[0046] In one embodiment of the present application, after the lysis cavity 110, the buffer cavity 210 and the detection cavity 310 are preloaded with corresponding reaction reagents, the internal air is extracted by a syringe or other vacuum extraction device to form a vacuum state, under the action of negative pressure, the sample in the lysis cavity 110 can automatically flow into the buffer cavity 210 through the communication of the connecting unit 400, and the sample in the buffer cavity 210 can automatically flow into the detection cavity 310 under the action of vacuum negative pressure, without the auxiliary driving of an external power source such as a syringe pump or a centrifuge, so that the operation is convenient.

[0047] It should be noted that, during the detection process, the sampling needle 500 and the connecting unit 400 are left in the internal cavity of the micro-fluidic detection chip, the air pressure between the lysis cavity 110, the buffer cavity 210 and the detection cavity 310 is balanced, the sampling port and the sample outlet are blocked, the internal cavities of the units are isolated from the external environment, and the sealing property of the micro-fluidic detection chip is ensured.

[0048] Figures 2 to 5 The schematic diagram of the sample flowing to the detection unit 300 for detection after the sample is injected into the lysis unit 100 is shown, specifically, Figure 2 Before the sample is injected into the lysis unit 100, the state of the micro-fluidic detection chip, Figure 3 After the sample enters the lysis unit 100 and is lysed in the lysis cavity 110, Figure 4 After the sample enters the buffer cavity 210 from the lysis cavity 110, the state of the sample in the buffer cavity 210, Figure 5 After the sample enters the detection cavity 310, the state of the sample in the detection cavity 310 for detection.

[0049] The detection process of the microfluidic detection chip in the application is as follows: Figures 2 to 5 The sample is sucked into the lysis cavity 110 for lysis under the action of vacuum negative pressure, the insertion state of the sampling needle 500 is maintained, the connecting unit 400 is simultaneously inserted into the lysis sample outlet 130 and the buffer sampling inlet 220, the lysis cavity 110 is communicated with the buffer cavity 210, the sample in the lysis cavity 110 automatically flows to the buffer unit 200 under the action of vacuum negative pressure of the buffer cavity 210, and enters the buffer cavity 210 for buffering and mixing; the insertion state of the connecting unit 400 in the lysis sample outlet 130 and the buffer sampling inlet 220 is maintained, the air pressure of the lysis cavity 110 and the buffer cavity 210 is balanced, the other connecting unit 400 is simultaneously inserted into the buffer sample outlet 230 and the detection sampling inlet 320, the buffer cavity 210 is communicated with the detection cavity 310, the sample in the buffer cavity 210 automatically flows to the detection unit 300 under the action of vacuum negative pressure of the detection cavity 310, and enters the detection cavity 310 for detection, so that the pretreatment, buffering and mixing and detection of the sample are completed.

[0050] The microfluidic detection chip further comprises a plurality of sealing units 600, which are sealed at the lysis sampling inlet 120, the buffer sampling inlet 220 and the detection sampling inlet 320. The sealing units 600 can be located outside the sampling inlet, or part of the sealing units 600 are inserted into the sampling inlet. By sealing the sampling inlet with the sealing units 600, the sealing effect of the microfluidic detection chip is optimized. During the detection process, the sampling needle 500 or the connecting unit 400 needs to be first inserted into the sealing unit 600 and then enter the sampling inlet. On the one hand, the sealing unit 600 supports and fixes the sampling needle 500 or the connecting unit 400. Since the sealing unit 600 protrudes from the surface of each unit, it can indicate the insertion position of the sampling needle 500 or the connecting unit 400, making the operation more convenient. On the other hand, it avoids the existence of gaps between the sampling inlet and the adjacent units, thereby reducing the sealing effect. The sealing units 600 can be fixed to each unit by adhesion, and the sealing units 600 can be made of rubber or silicone. In addition, it should be emphasized that the positions of the sampling inlet and the sample outlet correspond in two adjacent units, so that the connecting unit 400 can be simultaneously inserted. Therefore, the sealing units 600 can simultaneously seal the sampling inlet and the sample outlet that need to be communicated, so as to simultaneously optimize the sealing effect of the sampling inlet and the sample outlet. The sealing units 600 can also be inserted into the sample outlet or sealed outside the sample outlet.

[0051] The pyrolysis unit 100, buffer unit 200, and detection unit 300 can all be manufactured by machining or molding. The pyrolysis unit 100, buffer unit 200, and detection unit 300 are all composed of multiple layers of panels. The pyrolysis unit 100, buffer unit 200, and detection unit 300 can be made of plastic materials, such as PMMA. The panels of adjacent layers are aligned with each other and fixed by adhesive. PDMS (polydimethylsiloxane) material can also be used. PDMS is easy to process and mold, and has excellent electrical insulation and chemical inertness. It has high biocompatibility and can meet the requirements of different types of biological experiments. The panels of adjacent layers can be bonded and sealed by oxygen ion bonding.

[0052] Reference Figure 1 and Figure 7 In one embodiment of the present invention, the pyrolysis unit 100 includes a pyrolysis substrate 150 and a pyrolysis cover plate 160, which are stacked on top of each other. The pyrolysis substrate 150 is provided with a pyrolysis inlet 120, a pyrolysis outlet 130, a pyrolysis chamber 110 and a pyrolysis channel 170. The pyrolysis inlet 120 is connected to the pyrolysis chamber 110, and the pyrolysis chamber 110 and the pyrolysis outlet 130 are respectively connected to the two ends of the pyrolysis channel 170. After the pyrolysis substrate 150 and the pyrolysis cover plate 160 are combined and fixed, the pyrolysis inlet 120, the pyrolysis outlet 130 and the pyrolysis chamber 110 are sealed.

[0053] Reference Figure 1 , Figure 8 and Figure 9 The buffer unit 200 includes a buffer base plate 250, a buffer cover plate 260, and a buffer bottom plate 270. The buffer cover plate 260, the buffer base plate 250, and the buffer bottom plate 270 are stacked sequentially. The buffer cover plate 260 has a buffer inlet 220, the buffer base plate 250 has a buffer cavity 210, and the buffer bottom plate 270 has a buffer outlet 230. After the buffer cover plate 260, the buffer base plate 250, and the buffer bottom plate 270 are assembled and fixed, the buffer cavity 210, the buffer inlet 220, and the buffer outlet 230 are sealed. In one embodiment of the present invention, the buffer cover plate 260 has a plurality of buffer channels 280, one end of each buffer channel 280 is connected to the buffer inlet 220, and the other end of each buffer channel 280 is connected to different positions of the buffer cavity 210. The sample entering from the buffer inlet 220 is diverted by the plurality of buffer channels 280 and flows to the buffer cavity 210 from different buffer channels 280 respectively. Since the buffer channels 280 and the buffer cavity 210 are connected at different positions, the sample can flow into the buffer cavity 210 from different positions and directions to achieve sufficient mixing of the sample with the buffer reagent 240 in the buffer cavity 210. Furthermore, the sample can flow into the buffer cavity 210 from different buffer channels 280 at the same time, which improves the efficiency of the sample flowing into the buffer cavity 210 and the detection efficiency of the microfluidic detection chip.

[0054] In addition, in one embodiment, the lengths of the plurality of buffer channels 280 are equal, and the positions where the buffer channels 280 communicate with the buffer cavity 210 are symmetrically distributed relative to the center line of the buffer cavity 210, so that the flow distances of the samples from the buffer inlet 220 to the buffer cavity 210 are equal, the samples in different buffer channels 280 can flow into the buffer cavity 210 at the same time for buffering and mixing, and because the positions where the samples enter the buffer cavity 210 are symmetrical, the samples flowing into different regions of the buffer cavity 210 can mix together towards the central region of the buffer cavity 210, thereby improving the uniformity of the mixing of the samples and the buffer reagent 240.

[0055] Specifically, the buffer channels 280 can be provided in a multi-stage branched structure, such as a tree-shaped branch, and the buffer cavity 210 can be provided in a cylindrical cavity, and the positions where the buffer channels 280 communicate with the buffer cavity 210 are uniformly distributed along the outer periphery of the buffer cavity 210.

[0056] In addition, in one embodiment of the present application, the buffer unit 200 further has a mixing channel 290, which is provided on the buffer base plate 250 and located on the side of the buffer cavity 210 away from the buffer channels 280, and the two ends of the mixing channel 290 respectively communicate with the buffer cavity 210 and the buffer outlet 230, so that the samples in the buffer cavity 210 flow to the buffer outlet 230 through the mixing channel 290 and are fully mixed in the mixing channel 290 at the same time, thereby further improving the uniformity of the mixing of the samples and the buffer reagent 240. Specifically, the mixing channel 290 can be provided in a serpentine, S-shaped, or polyline shape; the buffer channels 280 and the mixing channel 290 are provided on different panels, which is conducive to the layout of the positions of the buffer channels 280 and the mixing channel 290, and the etching and molding of the buffer cover plate 260 and the buffer base plate 250.

[0057] In order to facilitate the communication function of the connecting unit 400, the buffer cover plate 260 is thinned in the region of the buffer inlet 220 to reduce the distance between the lysis outlet 130 and the buffer inlet 220, thereby facilitating the communication of the connecting unit 400 to the lysis outlet 130 and the buffer inlet 220; in addition, the buffer bottom plate 270 is thinned in the region of the buffer outlet 230 to reduce the distance between the buffer outlet 230 and the detection inlet 320, thereby facilitating the communication of the connecting unit 400 to the buffer inlet 220 and the detection inlet 320.

[0058] Referring to Figure 1 With Figure 10The detection unit 300 comprises a detection substrate 340 and a detection cover plate 350, the detection substrate 340 and the detection cover plate 350 are overlapped with each other, the detection inlet 320 and the detection cavity 310 are etched on the detection substrate 340, and the detection inlet 320 and the detection cavity 310 are sealed after the detection substrate 340 and the detection cover plate 350 are combined and fixed.

[0059] In one embodiment of the present application, the detection unit 300 has a plurality of detection cavities 310 and detection channels 360, both ends of each detection channel 360 are communicated with the detection inlet 320 and the detection cavity 310, and each detection cavity 310 contains a detection reagent 330, the sample can enter the corresponding detection cavity 310 through different detection channels 360 for detection, therefore, a plurality of detection tests can be simultaneously performed in the detection unit 300, and the detection efficiency is high. The detection channel 360 can also adopt a tree branch structure to ensure the consistency of the detection steps in different detection cavities 310.

[0060] It should be noted that the same detection reagent 330 can be placed in different detection cavities 310, the detection tests performed in different detection cavities 310 are the same, and the detection results can be the average value of the detection data in all detection cavities 310 to reduce the detection error. Alternatively, different detection reagents 330 are placed in at least two detection cavities 310, different detection tests are performed in different detection cavities 310 to detect different indexes of the sample respectively, and the detection efficiency is improved.

[0061] In addition, the detection substrate 340 is further provided with a waste liquid cavity 370, the waste liquid cavity 370 is communicated with the detection cavity 310, and the excess sample can flow from the detection cavity 310 into the waste liquid cavity 370 to avoid affecting the detection precision due to the different sample amounts. The samples in a plurality of detection cavities 310 can flow into one waste liquid cavity, or the waste liquid cavity 370 is provided with a plurality of waste liquid cavities, and the samples in each detection cavity 310 flow into different waste liquid cavities 370 respectively.

[0062] The microfluidic detection chip further comprises a heating unit 700, at least one of the lysis unit 100, the detection unit 200 and the detection unit 300 is connected with the heating unit 700, and the heating unit 700 provides good lysis conditions, buffer conditions and nucleic acid amplification detection conditions for the microfluidic detection chip through the heating action of the heating unit 700, so as to improve the lysis effect, the mixing uniformity or the sufficiency of the reaction with the detection reagent of the sample.

[0063] Figure 11 Taking the red dye as an example, the red dye is taken as a detection sample, the red dye sequentially passes through the lysis unit 100, the buffer unit 200 and the detection unit 300 through the communication of the connection unit 400, and the detection is realized. Specifically, Figure 11Fig. (a) in the figure is the initial state diagram of the microfluidic chip, Figure 11 Fig. (b) in the figure is the injection diagram of the red dye, the red dye is injected into the cracking unit 100 through the injection needle 500, Figure 11 Fig. (c) in the figure is that the red dye flows from the cracking unit 100 to the buffer unit 200 through the connection of the connection unit 400, Figure 11 Fig. (d) in the figure is that the red dye flows from the buffer unit 200 to the detection unit 300 through the connection of the connection unit 400. Thus, through the above detection process, the sample can automatically flow in the microfluidic chip after entering the microfluidic chip, and sequentially pass through the cracking unit 100, the buffer unit 200 and the detection unit 300 to complete the detection step by step.

[0064] Referring to Figure 12 The bacteria or virus can be used as the detection sample, the cracking reagent and the nucleic acid amplification detection reagent are preloaded in the microfluidic chip, and the cavities and channels in the cracking unit 100, the buffer unit 200 and the detection unit 300 are kept in a vacuum negative pressure state; the sample is introduced into the microfluidic chip through the injection needle 500, and the sample is gradually introduced into the cracking unit 100, the buffer unit 200 and the detection unit 300 under the driving of the pressure, and the cracking and nucleic acid amplification detection are completed; at the same time, the chamber preloaded with the nucleic acid amplification detection reagent without primers is used as an experimental control group, and after the detection is completed, the detection unit 300 preloaded with the nucleic acid amplification detection reagent generates obvious fluorescence signals. Figure 12 In the figure, the upper side is a schematic diagram of the fluorescence signal generated by the detection unit when the microfluidic chip detects the pseudomonas aeruginosa sample, and the lower side is a schematic diagram of the fluorescence background signal of the experimental control group.

[0065] In the present application, after the sample is filled, it can automatically flow in the microfluidic chip under the connection of the connection unit 400 and complete the detection, and the detection result is directly shown on the detection unit 300, so that the detection efficiency and practicability are high.

[0066] The embodiments of the present application are described in detail above in combination with the drawings, but the present application is not limited to the above embodiments, and various changes can be made within the knowledge range of those skilled in the art without departing from the purpose of the present application. In addition, the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

Claims

1. A microfluidic detection chip, characterized in that, The application relates to a sample injection device, which comprises the following parts: a cracking unit, which has a cracking cavity, a cracking sample inlet and a cracking sample outlet, the cracking cavity, the cracking sample inlet and the cracking sample outlet are sealed in the inside of the cracking unit, the cracking sample outlet and the cracking sample inlet are communicated with the cracking cavity, and the cracking cavity is used for containing a cracking reagent; a buffer unit, which has a buffer cavity, a buffer sample inlet and a buffer sample outlet, the buffer cavity, the buffer sample inlet and the buffer sample outlet are sealed in the inside of the buffer unit, the buffer sample inlet and the buffer sample outlet are communicated with the buffer cavity, and the buffer cavity is used for containing a buffer reagent; a detection unit, which has a detection cavity and a detection sample inlet, the detection cavity and the detection sample inlet are sealed in the inside of the detection unit, the detection cavity is communicated with the detection sample inlet, and the detection cavity is used for containing a detection reagent; wherein the cracking cavity, the buffer cavity and the detection cavity are all set as vacuum cavities; a connecting unit, which has an inner cavity, the inner cavity forms a first opening at one end of the connecting unit, a second opening is formed in the side of the connecting unit, the first opening and the second opening are communicated with the inner cavity, one end of the connecting unit has a sharp end, the first opening is located at the sharp end, and the sharp end can be inserted into the cracking unit, the buffer unit or the detection unit; wherein the connecting unit is provided with a plurality of connecting units, a sample injection needle can pierce the cracking sample inlet, so that the sample can be sucked into the cracking cavity under the action of vacuum negative pressure, one of the connecting units can be simultaneously inserted into the cracking sample outlet and the buffer sample inlet, so that the cracking sample outlet is communicated with the second opening, the buffer sample inlet is communicated with the first opening, and the sample in the cracking cavity automatically flows to the buffer unit under the action of vacuum negative pressure of the buffer cavity; one of the connecting units can be simultaneously inserted into the buffer sample outlet and the detection sample inlet, so that the buffer sample outlet is communicated with the second opening, the detection sample inlet is communicated with the first opening, and the sample in the buffer cavity automatically flows to the detection unit under the action of vacuum negative pressure of the detection cavity.

2. The microfluidic detection chip according to claim 1, characterized in that, The buffer unit further has a plurality of buffer channels, one end of the plurality of buffer channels is communicated with the buffer sample inlet, and the other end of the plurality of buffer channels is communicated to different positions of the buffer cavity.

3. The microfluidic detection chip according to claim 2, wherein, The lengths of the plurality of buffer channels are equal, and the communication positions of the buffer channels and the buffer cavity are symmetrically distributed relative to the center line of the buffer cavity.

4. The microfluidic detection chip of claim 1, wherein, The buffer unit further has a mixing channel, and two ends of the mixing channel are communicated with the buffer cavity and the buffer sample outlet respectively.

5. The microfluidic detection chip of claim 1, wherein, The detection unit has a plurality of detection cavities and a plurality of detection channels, and two ends of each detection channel are communicated with the detection sample inlet and the corresponding detection cavity.

6. The microfluidic detection chip of claim 1, wherein, The detection unit has a waste liquid cavity, and the waste liquid cavity is communicated with the detection cavity.

7. The microfluidic detection chip according to any one of claims 1 to 6, characterized in that, The application further comprises a plurality of sealing units, and the sealing units are sealed at the cracking sample inlet, the buffer sample inlet and the detection sample inlet.

8. The microfluidic detection chip of any one of claims 1 to 6, wherein, Also included is a heating unit, the cleaving unit and at least one of the detection units being connected to the heating unit.

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