Detection seat and POCT blood cell analyzer and motion mechanism thereof

Abstract

The application provides a motion mechanism and a POCT blood cell analyzer and a motion mechanism thereof, and the motion mechanism comprises a base frame, a push-pull motor, a push-pull motor and a push-pull guide assembly, the push-pull motor is fixed with the base frame and is provided with a push-pull lead screw, a push-pull nut is in threaded cooperation with the push-pull lead screw, the push-pull nut is provided with a detection seat, and the push-pull guide assembly is parallelly arranged at the side of the push-pull lead screw. Through the cooperation of the push-pull motor, the push-pull motor and the push-pull guide assembly, the detection seat and the pipettor can be driven to move to an operation position and a detection position to be detected, automatic distribution of blood samples and automatic addition of reagents can be realized, the structure is simple and convenient to operate, and the detection efficiency of the POCT can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of blood sample analysis, and in particular to a motion mechanism and a POCT blood cell analyzer. Background Art

[0002] Hematology analyzers are commonly used medical testing devices that measure parameters such as the number and proportion of blood cells (red blood cells, white blood cells, and platelets). Through blood analysis, they can identify microbial infections, diagnose and treat anemia, and diagnose blood disorders. With advancements in technology and scientific research, hematology analyzers have seen their functions expand, performance improve, and automation levels increase, leading to widespread clinical application.

[0003] Existing POCT blood cell analyzers require manual pre-treatment of blood samples before testing and analysis, and then place the blood samples in the analyzer for testing. This places high demands on the operator, and the experimental results are also affected by the operator's condition. The present invention provides a POCT motion mechanism that cooperates with a reagent kit to achieve full automation of the POCT blood cell analyzer. Summary of the Invention

[0004] The present invention provides a motion mechanism and a POCT blood cell analyzer, which can solve the problems of low detection efficiency and complex operation of blood cell analyzers in the prior art. The motion mechanism includes:

[0005] scaffolding;

[0006] A push-pull motor is fixed to the base frame and is provided with a push-pull screw rod;

[0007] A push-pull screw nut is threadably coupled to the push-pull screw rod, and a detection seat is provided on the push-pull screw nut;

[0008] The push-pull guide assembly is arranged parallel to the side of the push-pull screw rod.

[0009] According to a specific embodiment of the present application, the base frame includes a bottom plate and a side plate vertically connected to the bottom plate, the motion mechanism also includes a push-pull stroke detection optical coupler arranged on the bottom side of the side plate, and the push-pull nut is provided with a push-pull light blocking plate that matches the push-pull stroke detection optical coupler.

[0010] According to a specific embodiment of the present application, the motion mechanism further includes:

[0011] A translation motor is fixed to the base frame, the translation motor is located above the detection seat and is provided with a translation screw rod that is perpendicular to the push-pull screw rod;

[0012] A translation screw nut, threadably engaged with the translation screw rod;

[0013] The translation guide assembly is arranged parallel to the side of the translation screw rod.

[0014] According to a specific embodiment of the present application, the motion mechanism further includes a translation stroke detection optical coupler provided on the top side of the side plate, and the translation nut is provided with a translation light blocking plate that matches the translation stroke detection optical coupler.

[0015] According to a specific embodiment of the present application, the translation guide assembly includes a slide rail arranged between the side plates and located on the side of the translation screw, and a slider matching the slide rail, and the translation nut is arranged on the slider.

[0016] According to a specific embodiment of the present application, the translation nut is in a U-shape, including a lower plate, a vertical plate and an upper plate connected to each other, and the lower plate is arranged on the slider.

[0017] According to a specific embodiment of the present application, the motion mechanism further includes:

[0018] a first lifting motor, which is disposed on the upper plate and is provided with a first lifting screw rod passing through the upper plate;

[0019] a first lifting nut, threadably engaged with the first lifting screw rod, wherein the first lifting nut is provided with an air guide tube for connecting to a negative pressure air source;

[0020] The first lifting guide assembly is arranged parallel to the side of the first lifting screw and is connected to the lower plate and the upper plate.

[0021] According to a specific embodiment of the present application, the motion mechanism further includes a first lifting stroke detection optical coupler provided on the vertical plate, and the first lifting nut is provided with a first lifting light blocking plate that matches the first lifting stroke detection optical coupler.

[0022] According to a specific embodiment of the present application, the motion mechanism further includes:

[0023] a second lifting motor, which is arranged on the support plate at the top of the side plate and is provided with a second lifting screw passing through the support plate;

[0024] a second lifting screw nut, threadably engaged with the second lifting screw rod;

[0025] The second lifting guide assembly is arranged parallel to the side of the second lifting screw rod and passes through the support plate and the second lifting nut. The second lifting nut includes a shielding cover for matching with the detection seat.

[0026] In order to solve the above technical problems, a technical solution adopted by the present invention is: to provide a POCT blood cell analyzer, wherein the POCT blood cell analyzer includes the above-mentioned motion mechanism.

[0027] The beneficial effects of the present invention are as follows: different from the existing technology, the motion mechanism and POCT blood cell analyzer provided by the present invention can drive the detection seat and the pipette to move to the operating position and detection position to be detected through the mutual cooperation of the push-pull motor, the push-pull motor and the push-pull guide assembly, and can realize automatic distribution of blood samples and automatic addition of reagents. It has a simple structure and is easy to operate, which can improve the detection efficiency of POCT. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the invention. Those skilled in the art can also derive other drawings based on these drawings without inventive efforts, among which:

[0029] Figure 1 Schematic diagram of the three-dimensional structure of a POCT blood cell analyzer provided in an embodiment of the present invention;

[0030] Figure 2 This is a schematic diagram of a POCT blood cell analyzer provided by an embodiment of the present invention receiving and loading a reagent kit;

[0031] Figure 3 This is a schematic diagram of the internal structure of a POCT blood cell analyzer provided by an embodiment of the present invention;

[0032] Figure 4 Schematic diagram of the separation state of the test kit and the detection base provided by an embodiment of the present invention;

[0033] Figure 5 This is a top view of a partial structure of a detection base provided by an embodiment of the present invention;

[0034] Figure 6 This is a bottom view of a partial structure of a detection base provided by an embodiment of the present invention;

[0035] Figure 7 is a schematic diagram of the three-dimensional structure of the motion mechanism provided by an embodiment of the present invention;

[0036] Figure 8 It is a schematic diagram of the partial structure of the motion mechanism provided by an embodiment of the present invention;

[0037] Figure 9 1 is a schematic diagram of the exploded structure of the pipette provided in an embodiment of the present invention;

[0038] Figure 10 It is a schematic diagram of the assembly structure of the pipette provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0039] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0040] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0041] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features specified as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that ordinary technicians in this field can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0042] Please also refer to Figures 1 to 10 An embodiment of the present invention provides a POCT blood cell analyzer 10 , which includes a housing 11 , a detection seat 200 , a pipette 30 , and a motion mechanism 40 .

[0043] The housing 11 is provided with a display screen module 12, a door panel 13 and a handle portion 15. The display screen module 12 can be fixedly mounted on the upper side surface of the housing 11. The display screen module 12 can be arranged in an inclined shape for easy viewing. The display screen module 12 can be used to display the test results and perform touch operations. The test seat 200, the pipette 30 and the motion mechanism 40 are accommodated in the accommodation cavity formed by the housing 11. The housing 11 is also provided with an opening 14. The test seat 200 can slide in or out of the accommodation cavity through the opening 14, so that when the test seat 200 slides out of the accommodation cavity, the test seat 200 can add a sample to be tested or the sample that has completed the test operation can be replaced. The housing 11 is also provided with a door panel 13, which is used to cover the opening 14 and can be rotatably connected to the lower part or side of the housing 11. The handle portion 15 can be provided on the top of the housing 11 to make the POCT blood cell analyzer 10 portable and movable. Of course, the POCT blood cell analyzer can also be an analysis device that does not include a display screen module 12. The POCT blood cell analyzer can connect the main control module in the POCT blood cell analyzer 10 to a mobile terminal such as a mobile phone or tablet computer through communication methods such as Bluetooth, WIFI, NFC, etc., and display the test results and perform corresponding touch operations through the mobile phone or tablet computer.

[0044] The detection seat 200 is disposed within the housing 11 and is configured to receive the reagent kit 100. The detection seat 200 is equipped with one or more optical detection assemblies 220. When the door panel 13 is opened relative to the housing 11, the detection seat 200 can be moved out of the housing 11 via a sliding assembly or hinge assembly within the housing 11 to receive the reagent kit 100. The detection seat 200 is equipped with several impedance detection assemblies 210 and / or several optical detection assemblies 220. The impedance detection assemblies 210 and optical detection assemblies 220 can be used to perform impedance detection and optical detection on blood cells. Each impedance detection assembly 210 and each optical detection assembly 220 can detect an independent item to obtain a specific blood cell parameter value.

[0045] The pipette 30 is arranged in the shell 11 and can be located above the detection seat 200, and is used to be connected to the pipette head 114 (such as a tip head) to perform pipetting operations on the sample liquid or reagent liquid in the reagent kit 100. The pipetting operation refers to the operation of aspirating, moving, and spitting. The pipette 30 can also be used to repeatedly aspirate and spit / oscillate the liquid to form an aspirating and spitting / oscillating mixing effect. The pipette 30 provided by the present invention is connected with a pipette head 114, and the pipette head 114 has a certain volume. During the pipetting operation, the sample liquid or reagent liquid will remain in the pipette head 114 and will not enter the interior of the pipette 30. After completing the aspirating, moving, and spitting operations, when the sample liquid or reagent liquid needs to be replaced, the used pipette head 114 can be discarded and the unused pipette head 114 can be reinstalled, so that the new sample liquid or reagent liquid can be pipetted, thereby without contaminating the pipette 30. Therefore, there is no need to clean the pipette 30 after each use, eliminating the complicated cleaning components and cleaning processes, and improving the detection efficiency.

[0046] Please also refer to Figures 4 to 6 An embodiment of the present invention provides a detection base 20, which includes an upper shell 21 and a lower shell 22, a shielding box 200, a radiator 240 and a cooling fan 250. The upper shell 21 and the lower shell 22 cooperate with each other to form a shell for accommodating the shielding box 200, the Peltier 230, the radiator 240 and the fan. The side surface of the shielding box 200 is sequentially provided with the Peltier 230, the radiator 240 and the cooling fan 250. The radiator 240 includes a heat absorbing plate that is bonded to the Peltier 230 and a plurality of cooling fins 245 provided on a side of the heat absorbing plate away from the Peltier 230. The cooling fan 250 is fixed to the outer end of the cooling fin 245. Among them, one end of the Peltier 230 for cooling is connected to the shielding box 200, and is used to cool the reagent to be tested in the reagent kit 100 by cooling, so that the reagent to be tested has a temperature suitable for testing. The other end of the Peltier 230 for heating is connected to the heat sink fin 245 through a heat absorbing plate, and is used to conduct the heat generated by the end of the Peltier 230 close to the radiator 240 out of the shell through the fan, so as to avoid heat accumulation in the shell affecting the cooling effect of the side of the Peltier 230 away from the radiator 240 and having an adverse effect on the test results.

[0047] Furthermore, ventilation holes are provided on the outer shell of the detection seat 20 at positions corresponding to the cooling fan 250, wherein an air inlet 251 is provided at the side position of the outer shell corresponding to the cooling fan 250, and an air outlet 252 is provided at the top and bottom positions of the outer shell corresponding to the cooling fan 250 and the radiator 240. During the detection process, air enters the accommodating cavity through the air inlet 251 by negative suction under the action of the cooling fan 250, and the gas in the accommodating cavity passes through the cooling fan 250 and the radiator 240 in turn, and the heat is taken out through the air outlet 252 on the top and bottom surfaces of the outer shell.

[0048] Furthermore, a heating film 260 may be provided at the bottom of the shielding box 200 to conduct the heat generated by the heating film 260 through the shielding box 200 to the reagent chamber 100 to raise the temperature of the reagent to be tested, so that the reagent to be tested reaches a temperature suitable for testing. This can solve the problem that existing POCT blood cell analyzers rely on the constant temperature environment of the laboratory and are not convenient for use in the natural environment. The POCT blood cell analyzer provided by the present invention can independently heat or cool by providing a Peltier 230 or a heating film 260 on the detection base 20, reducing the impact of the natural environment temperature on the test results.

[0049] Furthermore, in the present application, an opening is provided on the upper shell 21 of the shielding box 200 to reveal the top of the shielding box 200, so that when the shielding box 200 is placed in the housing 11 of the hematology analyzer, the detection reagent can be detected by the pipette 30 and other mechanisms.

[0050] The shielding box 200 is a box with an open top made of metal sheet. The shielding box 200 is provided with a recess for accommodating the reagent kit 100. A resilient elastic plate or plastic sheet is attached to the outer surface of the recess in the shielding box 200. When the reagent kit 100 is accommodated in the recess in the shielding box 200, the elastic contraction of the elastic plate or plastic sheet secures the reagent kit 100 in the recess, preventing the reagent kit 100 from affecting the detection process due to positional changes. The top of the sidewall of the shielding box 200 is also provided with a recessed buckle 227. When the reagent kit 100, which has an elastic snap-on piece 150 on the side, is installed in the shielding box 200, the recessed buckle 227 engages with the elastic snap-on piece 150.

[0051] Furthermore, in this embodiment, the POCT blood cell analyzer also includes a shielding cover 445 arranged above the detection base 20. The shielding cover 445 can be snapped or fastened with the opening on the upper surface of the detection base 20 or the opening above the periphery of the upper surface under the driving of external force, so that the detection base 20 and the shielding cover 445 form a closed metal shell as a shielding body to prevent electromagnetic interference generated by the battery, power supply, motor or other electronic components during operation from affecting the detection process and affecting the accuracy of the detection results.

[0052] A first optical detection component and a second optical detection component are also provided in the shielding box 200, wherein the first optical detection component includes a first transmitting end 221 and a first receiving end 222 arranged at intervals, and a first optical detection position 223 is formed between the first transmitting end 221 and the first receiving end 222; the second optical detection component includes a second transmitting end 224 and a second receiving end 225 arranged at intervals, and a second optical detection position 226 is formed between the second transmitting end 224 and the second receiving end 225; and the first optical detection position 223 and the second optical detection position 226 are staggered. This application does not limit the specific positions of the first optical detection position 223 and the second optical detection position 226, as long as it is ensured that there is no position overlap between the first optical detection position 223 and the second optical detection position 226, so as to avoid the light emitted by the first transmitting end 221 of the first optical detection component being received by the second receiving end 225 of the second optical detection component, affecting the detection results.

[0053] Specifically, the first emitting end 221 of the first optical detection component can be a light source that emits laser, and the second emitting end 224 of the second optical detection component can be a light source that generates infrared light. The first emitting end 221 of the first optical detection component and the second emitting end 224 of the second optical detection component are respectively arranged on the first side and the third side, two opposite sides of the shielding box 200, and the first receiving end 222 of the first optical detection component and the second receiving end 225 of the second optical detection component are respectively arranged at positions corresponding to the first emitting end 221 and the second emitting end 224 between the first side and the third side. In order to avoid the light generated by the first optical detection component and the light generated by the second optical detection component from crossing and overlapping, a light blocking portion can be provided between the first optical detection component and the second optical detection component to achieve a physical barrier between the first optical detection position 223 and the second optical detection position 226; or the first receiving end 222 of the first optical detection component and the second receiving end 225 of the second optical detection component are staggered; or the first optical detection component and the second optical detection component are connected to the side or side part area to avoid the light generated by the first detection area and the second detection area interfering with each other and affecting the detection results.

[0054] Furthermore, when a light shielding portion is provided in the detection base 20 , the light shielding portion may be connected to any one of the first transmitting end 221 and the first receiving end 222 and any one of the second transmitting end 224 and the second receiving end 225 .

[0055] Furthermore, the detection base in this embodiment may further include a plurality of optical detection components, each of which includes a transmitting end and a receiving end that are spaced apart.

[0056] The transmitting end and receiving end of each optical detection component in the multiple optical detection components cooperate to form multiple optical detection positions. To ensure that the transmitting end and receiving end of each optical detection component do not interfere with the transmitting end and receiving end of other optical components when testing a blood sample, thereby affecting the accuracy of the test results, light shields can be provided between the multiple optical detection positions to physically isolate the multiple optical detection positions from each other. For example, light-blocking components such as metal shields or plastic sheets can be provided between the multiple optical detection positions. Of course, the multiple optical detection positions can also be staggered to prevent interference between the multiple optical detection positions.

[0057] Specifically, in this embodiment, multiple optical detection sites can be used to detect multiple parameters in a blood sample, including but not limited to the concentration values ​​of components such as C-reactive protein (CRP), high-sensitivity C-reactive protein (hs-CRP), D-dimer (D-Dimer), transferrin (TRF), serum amyloid protein (SAA), procalcitonin (PCT), glycosylated hemoglobin (HbA1C), and adiponectin (ADPN). By detecting the concentration values ​​of different components in the blood sample, the values ​​are displayed to the user for evaluation. Specifically, in this embodiment, the user can appropriately increase or decrease the number of optical detection modules as needed to meet detection needs.

[0058] Optionally, each optical detection module is an integrated component, and multiple fixed positions are set on the detection base. When it is necessary to add or remove optical detection modules, it is only necessary to install multiple optical detection modules on the detection base or remove them from the mounting base to meet the needs of adding or removing optical modules.

[0059] In the present application, two or more optical detection components 220 can realize the detection of two or more independent items to obtain specific blood cell parameter values.

[0060] In the present application, two or more optical detection components can realize the detection of two or more independent items to obtain specific blood cell parameter values.

[0061] In the present application, the conductive support 220 is arranged to protrude from the side wall of the shielding box 200. Preferably, one end of the conductive support 220 is snap-fitted or fixedly connected to the shielding box 200, and the other end of the conductive support 220 protrudes from the side wall of the shielding box 200. The conductive support 220 can support the conductive column 125 to form an electrical overlap.

[0062] Optionally, the conductive support 220 is a plate-shaped conductive sheet arranged on the side wall of the shielding box 200, with a limiting groove provided on the plate-shaped conductive sheet, and a protrusion is provided at one end of the conductive column, which is used to cooperate with the limiting groove of the conductive column to form an electrical overlap.

[0063] In another embodiment of the present application, the conductive support 220 can also be a conductive seat arranged on the side wall of the shielding box 200, and the conductive seat has a recessed portion, which is any one or more of a V-shaped recessed portion, an arc-shaped recessed portion, a U-shaped recessed portion, an inverted trapezoidal recessed portion or a rectangular recessed portion, so that when the test kit 100 with a conductive column 125 on the side is installed in the shielding box 200, the recessed portion of the conductive support 220 supports the conductive column 125 to form an electrical overlap.

[0064] Please also refer to Figures 4 to 6 The present invention provides a test kit 100, which can be a disposable test kit 100 or a reusable test kit 100. The test kit 100 includes a box body 110, a plurality of impedance detection cells 120, a plurality of optical detection cells 130, and a reagent cell 140. The test kit 100 can have an asymmetric structure to facilitate foolproof installation within a shielding box 200 on a detection base 20.

[0065] In one embodiment, the box body 110 may be generally rectangular and may be provided with a first insertion hole 111 for receiving a sample tube 113. Alternatively, the box body 110 may be directly connected to a sample reservoir, such as by an integral connection or a sleeve connection, and the sample tube 113 may be provided to receive a sample, such as a blood sample. The box body 110 may also be provided with a second insertion hole 112 for receiving a pipette tip 114. The pipette tip 114 is configured to be assembled with a pipette 30 to facilitate pipetting operations.

[0066] The box body 110 is also provided with several impedance detection cells 120 for performing impedance detection on the diluted blood sample. There is at least one impedance detection cell 120, and each of the impedance detection cells 120 has an opening connected to a conductive column 125. Upon receiving pressure from the pipette 30, the impedance detection cell 120 causes the liquid within the impedance detection cell 120 to flow, thereby generating impedance information detected by the conductive column 125 to analyze the composition of the sample to be tested. The box body 110 is also provided with several optical detection cells 130 for performing photoelectric detection on the diluted blood sample. There is at least one optical detection cell 130, which is used to coordinate transmitted light photoelectric detection and / or scattered light photoelectric detection. The optical detection cell 130 can be made of optical plastic, transparent plastic, or glass.

[0067] The box body 110 is also provided with a reagent pool 140 for installing reagents, and the reagents may be hemolytic agents, antibody reagents, diluents, etc. There may be multiple reagent pools 140 to install multiple reagents, or there may be only one reagent pool 140.

[0068] The box body 110 is further provided with an exposed electrode conductive device, wherein the electrode conductive device is provided at a position corresponding to the conductive support 220 of the shielding box 200 , wherein the electrode conductive device is a conductive column 125 .

[0069] The test kit 100 provided by the present invention can support impedance detection and / or colorimetric detection and / or turbidimetric detection of blood samples by providing a plurality of impedance detection cells 120, a plurality of optical detection cells 130, and a plurality of reagent cells 140. Each optical detection cell 130 and each reagent cell 140 can cooperate to detect an independent project to obtain a specific blood cell parameter value. The test items are numerous and the test items are scalable. The dilution of the sample to be tested and the pre-treatment of adding reagents can be prepared by pre-installed reagents in the reagent cell 140, thereby optimizing the POCT detection efficiency. In addition, the test kit 100 itself can serve as a waste liquid collector, thereby eliminating the reagent barrel and waste liquid barrel that need to be connected to the traditional blood cell analyzer, making the POCT blood cell analyzer 10 more portable.

[0070] Please also refer to Figures 1 to 10 An embodiment of the present invention provides a POCT blood cell analyzer 10 , which includes a housing 11 , a detection seat 200 , a pipette 30 and a motion mechanism 40 .

[0071] In the present application, the pipette 30 is arranged in the housing 11 and can be located above the detection seat 200, for connecting the pipette head 114 (such as a tip head) to perform pipetting operations on the reagent box 100. The pipette 30 may include a motor, a cavity, and a piston, and the motor is used to drive the piston to reciprocate in the cavity to perform pipetting operations. Among them, the motion mechanism 40 is used to make the pipette 30 and the detection seat 200 move relative to each other. The motion mechanism 40 can be a three-axis motion system to drive the pipette 30 to move in the X, Y, and Z directions. Of course, the implementation method of the motion mechanism 40 is not limited to this. The motion mechanism 40 can also drive the detection seat 200 to move relative to the pipette 30 or drive the pipette 30 and the detection seat 200 to move at the same time.

[0072] In one embodiment, the motion mechanism 40 includes a first motor transmission assembly 410, a second motor transmission assembly 420, and a third motor transmission assembly 430. The first motor transmission assembly 410 is used to drive the detection seat 20 to move in the X direction; the second motor transmission assembly 420 is used to drive the pipette 30 to move in the Y direction; the third motor transmission assembly 430 is used to drive the pipette 30 to move in the Z direction. The first motor transmission assembly 410, the second motor transmission assembly 420, and the third motor transmission assembly 430 may include a motor, a screw rod, and a nut. The output end of the motor 401 is fixedly connected to the screw rod, and the nut cooperates with the screw rod. The nut drives the detection seat 200 or the pipette 30 to slide accordingly in the X, Y, and Z directions.

[0073] In one embodiment, the hematology analyzer further includes a base frame 15 disposed within the outer shell 11 and a support plate 153 connected to the base frame 15. A first transmission assembly is fixedly disposed on the base frame 15, and a first motor transmission assembly 410 includes a push-pull motor 411, a push-pull screw rod 412, a push-pull screw nut 413, and a push-pull guide assembly 415. The output end of the push-pull motor 411 is fixedly provided with a push-pull screw rod 412; the push-pull screw rod 412 is threadedly engaged with the push-pull screw nut 413, and the push-pull screw nut 413 is provided with a detection seat 20, so that the push-pull screw rod 412 can drive the push-pull screw nut 413 to perform push-pull motion in the X direction under the drive of the push-pull motor 411, thereby driving the detection seat 20 to move in the X direction.

[0074] The first motor transmission assembly 410 also includes a push-pull guide assembly 415, which is arranged parallel to the side of the push-pull screw rod 412. Specifically, there can be two push-pull guide assemblies 415, which are respectively arranged on the base frame 15 on both sides of the push-pull screw rod 412 and connected to the push-pull screw nut 413.

[0075] Among them, the base frame 15 includes a bottom plate 151 and a side plate 152 vertically connected to the bottom plate 151, and the motion mechanism 40 also includes a push-pull stroke detection optical coupler 414 arranged on the bottom side of the side plate 152, and a push-pull nut 413 is provided with a push-pull light blocking plate 416 matching the push-pull stroke detection optical coupler 414, which is used to move to the push-pull stroke detection optical coupler 414 through the push-pull light blocking plate 416 when the detection seat 20 slides linearly relative to the base frame 15 to sense the position state of the detection seat 20.

[0076] Specifically, there can be two push-pull light shields 416, which are relatively arranged at one end close to the detection position of the detection base 20 to be detected, or there can be four push-pull light shields 416, which are relatively arranged at one end close to the detection position of the detection base 20 and one end away from the detection position of the detection base 20 to be detected. When the push-pull light shield 416 moves to the non-optical coupling detection area, its receiving end can receive the signal sent by the transmitting end. When the push-pull light shield 416 moves to the optical coupling detection area, its receiving end cannot receive the signal sent by the transmitting end, thereby sensing a specific position, and thus controlling the detection base 20 to move to the target position to be detected under the drive of the push-pull motor 411.

[0077] The second transmission component includes a translation motor 421, which is fixedly arranged on the base frame 15. The translation motor 421 is located above the detection seat 20 and is provided with a translation screw rod 422 that is perpendicular to the push-pull screw rod 412. A translation nut 423 is in threaded fit with the translation screw rod 422. Among them, the translation nut 423 includes a lower plate 4231, a vertical plate 4232, and an upper plate 4233 that are connected in sequence. The output end of the translation motor 421 is fixedly connected to the translation screw rod 422. The translation screw rod 422 is in threaded fit with the vertical plate 4232 at the bottom position of the vertical plate 4232 of the translation nut 423. Thus, the translation screw rod 422 can drive the translation nut 423 to perform a translation movement in the Y direction under the drive of the translation motor 421.

[0078] The second motor transmission component 420 further includes a translation guiding component, which is arranged in parallel on the side of the push-pull screw rod 412. Specifically, the push-pull guiding component 415 is a slide rail 425 that is arranged in parallel with the translation screw rod 422 and is located on the side of the translation screw rod 422, and a slider 426 that matches the slide rail 425. The translation nut 423 is arranged on the slider 426 to drive the translation nut 423 to move in the Y direction along the translation guide rail.

[0079] The second motor transmission component 420 further includes a translation stroke detection opto-coupler 424 arranged on the top side of the side plate 152. A translation light-blocking piece (not labeled in the figure) that matches the translation stroke detection opto-coupler 424 is arranged on the translation nut 423, which is used to sense the position state of the translation nut 423 when the detection seat 20 slides linearly relative to the base frame 15 by moving the translation light-blocking piece to the translation stroke detection opto-coupler 424.

[0080] Specifically, in this embodiment, the translation nut 423 is in a U shape. The translation nut 423 includes a lower plate 4231, a vertical plate 4232, and an upper plate 4233 that are connected in sequence. The lower plate 4231 is arranged on the slider 426.

[0081] In this embodiment, the pipette further includes an air guide tube 115 and a carrier piece that is assembled and connected to the air guide tube. The air guide tube 115 is connected to a gas source. The end of the air guide tube 115 is used to connect a disposable pipette tip 114. The carrier piece surrounds the outer periphery of the air guide tube 115. The carrier piece can drive the air guide tube 115 to move relative to the disposable pipette tip 114 so that the air guide tube 115 and the disposable pipette tip 114 are sleeved with each other or separated.

[0082] Among them, the driven part is a double-layer structure inside and outside. A cavity is also arranged on the driven part. The air guide tube is accommodated in the cavity of the driven part. One end of the air guide tube is exposed outside the cavity of the driven part. The other end of the air guide tube is connected to an air pump and an air valve. When the air guide tube is not assembled with the pipette tip, the air guide tube can be assembled with the pipette tip under the action of the driving part. After the assembly connection is completed, the air guide tube can generate positive pressure or negative pressure at the end of the air guide tube through the air pump to perform liquid suction or liquid ejection operations.

[0083] Furthermore, the carrier is a third transmission assembly, which includes a first lifting motor 431, the first lifting motor 431 is arranged on the upper plate 4233 and is provided with a first lifting screw rod 432 passing through the upper plate 4233; the first lifting screw rod 432 is threadedly engaged with the first lifting nut 433, and the first lifting nut 433 is provided with an air guide tube 115 for connecting to a negative pressure air source, and the end of the air guide tube 115 is used to connect a disposable pipette head 114.

[0084] It should be noted that, in this embodiment, the connection method between the first driving member and the first driven member is not limited to the connection method between the lifting screw and the lifting nut, but can also be other transmission structures that can transmit in the vertical direction, such as chains and gears, slide rails and sliders, gears and racks, conveyor belts and rotating shafts and other transmission structures. In this application, only the transmission structure of the lifting screw and the lifting nut is used as an embodiment, and this embodiment does not constitute a limitation on the technical solution protected by this application.

[0085] Furthermore, in this embodiment, the first lifting motor 431 is disposed on the upper plate 4233 , and the first lifting screw 432 passes through the upper plate 4233 .

[0086] The first lifting guide assembly is arranged parallel to the side of the first lifting screw 432 and connected to the lower plate 4231 and the upper plate 4233. The motion mechanism 40 also includes a first lifting stroke detection optical coupler provided on the vertical plate 4232, and a first lifting light shielding plate is provided on the first lifting screw nut 433 to match the first lifting stroke detection optical coupler.

[0087] The POCT blood cell analyzer in this embodiment can drive the detection seat 20 to move in the X direction to the position to be detected through the first motor transmission component 410; the second motor transmission component 420 drives the pipette 30 to move in the Y direction through the vertical plate 4232 of the translation nut 423, and the third motor transmission component 430 drives the pipette 30 to move in the Z direction through the upper plate 4233 of the translation nut 423, thereby driving the pipette 30 to move above the detection seat 20. By driving the air guide tube 115 on the first lifting nut 433 to move downward in the Y direction, the air guide tube 115 can be connected to the pipette head 114 to perform pipetting operations on the reagent kit 100, thereby realizing fully automatic pipetting and detection operations without manual intervention.

[0088] In this embodiment, the motion mechanism 40 also includes a fourth transmission assembly 440, which includes a second lifting motor 441, which is arranged on the support plate 153 at the top of the side plate 152 and is provided with a second lifting screw 442 that passes through the support plate 153; the second lifting screw 442 is threadedly engaged with the second lifting nut 443; and a second lifting guide assembly 444, which is arranged parallel to the side of the second lifting screw 442 and passes through the support plate 153 and the second lifting nut 443; wherein the second lifting nut 443 also includes a shielding cover 445 for matching with the detection base 20. The shielding cover 445 is used to be snap-fitted or fastened to the upper surface of the detection base 20 with an opening or the outer periphery of the upper surface under the drive of the second lifting motor 441, so that the detection base 20 and the shielding cover 445 form a closed metal shell as a shielding body to prevent electromagnetic interference generated by the battery, power supply, motor or other electronic components during operation from affecting the detection process and affecting the accuracy of the detection results.

[0089] Please also refer to Figures 1 to 10 An embodiment of the present invention provides a pipette 30, which includes the aforementioned moving component and an electromagnetic component connected to the moving component, wherein the electromagnetic component includes an electromagnetic mounting plate 510 and an electromagnet 520 arranged on the electromagnetic mounting plate 510, and the electromagnet 520 is used to operate when energized to remove the disposable pipette tip 114.

[0090] In one embodiment of the present application, the electromagnet 520 includes a striker 521 and a linkage 522. The striker 521 is arranged above the linkage. The striker 521 can move up and down when powered on and off to drive the linkage 522 to move so as to achieve the installation and removal of the pipette head 114.

[0091] Specifically, the striker 521 can move downward when energized, and the linkage 522 is arranged at a position opposite to the striker 521. The linkage 522 also includes a socket portion 525 that is socketed with the air guide tube 115. The air guide tube 115 is accommodated in the socket portion 525. When the electromagnet 520 is energized, the striker 521 can hit the linkage 522 to cause the pipette head 114 socketed on the end of the air guide tube 115 to fall off.

[0092] In another embodiment of the present application, the striker 521 can also be set in a direction horizontal to the linkage 522, and be arranged side by side with the linkage 522. The striker 521 can move toward the direction of the linkage 522 when energized, and abut against the outer periphery of the upper surface of the pipette head 114 or the socket part 525 outside the air guide tube 115 sleeved in the linkage 522, so that when the air guide tube 115 is driven to move upward, the striker 521 can abut against the pipette head 114 or the socket part 525, thereby causing the disposable pipette head 114 sleeved on the end of the air guide tube 115 to fall off.

[0093] Among them, in this application, the pipette head 114 is disposable. In this way, the used disposable pipette head 114 on the air guide tube 115 can be removed, and the air guide tube 115 is further driven to move to a new position through the aforementioned motion component. By driving the air guide tube 115 to move, the unused pipette head 114 is inserted to facilitate a new round of pipetting operation on the reagent kit 100.

[0094] In this embodiment, the electromagnet mounting plate 510 is provided with a linkage position detection optical coupler 530, and the linkage 522 is further provided with a linkage light shield 524 that matches the linkage position detection optical coupler 530. The electromagnet mounting plate 510 is provided with a through slot 515, and the linkage light shield 524 is inserted into the through slot 515. The linkage light shield 524 is exposed at one end of the electromagnet mounting plate 510 and is opposite to the linkage position detection optical coupler 530. When the disposable pipette tip 114 is plugged into the end of the air guide tube 115, the linkage light shield 524 enters the detection area of ​​the linkage position detection optical coupler 530, thereby identifying whether the disposable pipette tip 114 has been plugged into the air guide tube 115. This avoids the situation where the disposable pipette tip 114 is not plugged into the air guide tube 115 and is not effectively pipetted, thereby ensuring the normal and continuous pipetting operation, the validity of the sample, and the accuracy of the test results. The electromagnet 520 further includes a return spring 523, which is sleeved on the striker 521. When the electromagnet 520 is energized, the return spring 523 is forced to contract under the action of the electromagnet 520. Due to the contraction of the return spring 523, the striker 521 falls under the action of gravity, thereby pressing against the position where the air guide tube 115 and the pipette head 114 are engaged, thereby removing the pipette head 114 sleeved on the air guide tube 115. When the electromagnet 520 is de-energized, the return spring 523 is in a natural state, and the return spring 523 recovers its deformation, thereby returning the striker 521 to its initial position. Optionally, the return spring 523 is a cylindrical spring or a conical spring.

[0095] In one embodiment of the present invention, the pipette 30 further includes a motor (not shown), a cavity (not shown), and a piston (not shown). The motor is used to drive the piston to reciprocate within the cavity to perform a pipetting operation. The pipette 30 can also be used to provide positive pressure to promote the flow of liquid within the impedance detection cell 120 of the reagent kit 100, thereby facilitating the impedance detection operation. The pipette 30 is also used to perform aspiration / oscillation to achieve a mixing operation; the pipette 30 can also be used to inject bubbles into the liquid to achieve a mixing operation.

[0096] The POCT blood cell analyzer provided in an embodiment of the present invention integrates the supporting components (impedance detection cell 120, optical detection cell 130) and various reagents required for the test system on the test kit 100. The test kit 100 is disposable and the bulky cleaning system can be removed, thereby significantly reducing the number of components of the entire instrument. This not only reduces the size of the analyzer and makes it more portable, but also saves the time spent on cleaning the impedance detection cell 120 and the optical detection cell 130 by replacing the sample by replacing the test kit 100, thereby greatly improving the detection efficiency.

[0097] The embodiment of the present invention further provides a detection method based on the aforementioned POCT blood cell analyzer 10, the method comprising:

[0098] The reagent kit 100 is received and loaded into the detection seat 200, wherein the reagent kit 100 includes a plurality of impedance detection cells 120 and / or a plurality of optical detection cells 130 for performing impedance and / or photoelectric detection. The reagent kit 100 also includes a plurality of reagent cells 140 for storing a plurality of reagents. The reagent kit 100 is also provided with a sample tube 113, and the sample tube 113 contains a blood sample.

[0099] The blood sample is distributed into a specific reagent pool 140 by the pipette 30, that is, the blood is automatically separated by the device;

[0100] The reagents in other reagent pools 140 are added to the specific reagent pool 140 by the pipette 30 for mixing to form a sample to be tested, that is, the reagents are automatically added by the device;

[0101] The sample to be tested is moved into the impedance detection cell 120 and / or the optical detection cell 130 by the pipette 30 to perform impedance detection and / or optical detection.

[0102] The POCT blood cell analyzer and detection method provided by the present invention can realize automatic distribution of blood samples and automatic addition of reagents through the cooperation of the motion mechanism 40 and the pipette 30, thereby optimizing the efficiency of POCT detection. Existing detection methods require manual addition of reagents, manual mixing, and then pouring into the instrument for detection.

[0103] The step of distributing the blood sample to the specific reagent pool 140 by the pipette 30 includes:

[0104] The pipette 30 is plugged and docked with the pipette tip 114 pre-installed on the reagent kit 100 so that the pipette tip 114 only contacts the blood sample, reagent and sample to be tested during the pipetting operation, thereby avoiding contamination of the pipette 30 .

[0105] Among them, when performing impedance detection, the pipette 30 is used to provide positive pressure to promote the flow of the sample to be tested in the impedance detection pool 120. By reusing the pipette 30, the need for additional negative pressure devices for impedance detection drainage in the prior art can be reduced, thereby reducing product components.

[0106] The POCT blood cell analyzer and detection method provided in this embodiment can realize automatic distribution of blood samples and automatic addition of reagents through the cooperation of the motion mechanism and the pipette, thereby improving the efficiency of POCT detection.

[0107] The above are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention description and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A motion mechanism of a POCT blood cell analyzer, characterized in that: include: scaffolding; A push-pull motor is fixed to the base frame and is provided with a push-pull screw rod; A push-pull screw nut is threadably coupled to the push-pull screw rod, and a detection seat is provided on the push-pull screw nut; A push-pull guide assembly is arranged parallel to the side of the push-pull screw rod; The motion mechanism further includes a fourth transmission assembly, which includes a second lifting motor, a second lifting nut and a second lifting guide assembly; The base frame includes a bottom plate and a side plate vertically connected to the bottom plate, the second lifting motor is arranged on the support plate on the top of the side plate and is provided with a second lifting screw rod passing through the support plate; the second lifting screw rod is threadedly engaged with the second lifting nut; the second lifting guide assembly is arranged parallel to the side of the second lifting screw rod and passes through the support plate and the second lifting nut; Among them, the second lifting nut also includes a shielding cover for matching with the detection seat of the POCT blood cell analyzer. The shielding cover is used to snap or fasten with the upper surface of the detection seat with an opening or the outer periphery of the upper surface under the drive of the second lifting motor, so that the detection seat and the shielding cover form a shielding body.

2. The motion mechanism according to claim 1, characterized in that: The motion mechanism further includes a push-pull stroke detection optical coupler provided on the bottom side of the side plate, and the push-pull nut is provided with a push-pull light blocking sheet that matches the push-pull stroke detection optical coupler.

3. The motion mechanism according to claim 2, characterized in that: The motion mechanism further comprises: A translation motor is fixed to the base frame, the translation motor is located above the detection seat and is provided with a translation screw rod that is perpendicular to the push-pull screw rod; A translation screw nut, threadably engaged with the translation screw rod; The translation guide assembly is arranged parallel to the side of the translation screw rod.

4. The motion mechanism according to claim 3, characterized in that: The motion mechanism further includes a translation stroke detection optical coupler provided on the top side of the side plate, and the translation nut is provided with a translation light blocking piece that matches the translation stroke detection optical coupler.

5. The motion mechanism according to claim 3, characterized in that: The translation guide assembly includes a slide rail arranged between the side plates and located on the side of the translation screw rod, and a slider matched with the slide rail, and the translation nut is arranged on the slider.

6. The motion mechanism according to claim 5, characterized in that: The translation nut is in a U-shape and includes a lower plate, a vertical plate and an upper plate that are connected to each other, and the lower plate is arranged on the slider.

7. The motion mechanism according to claim 6, characterized in that: The motion mechanism further comprises: a first lifting motor, which is disposed on the upper plate and is provided with a first lifting screw rod passing through the upper plate; a first lifting nut, threadably engaged with the first lifting screw rod, wherein the first lifting nut is provided with an air guide tube for connecting to a negative pressure air source; The first lifting guide assembly is arranged parallel to the side of the first lifting screw and is connected to the lower plate and the upper plate.

8. The motion mechanism according to claim 7, characterized in that: The motion mechanism further includes a first lifting stroke detection optical coupler provided on the vertical plate, and the first lifting nut is provided with a first lifting light blocking piece matching the first lifting stroke detection optical coupler.

9. A POCT blood cell analyzer, characterized in that: The POCT blood cell analyzer comprises the movement mechanism according to any one of claims 1 to 8.

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