A double-layer microcapsule bio-soil conditioner and a preparation method thereof

Abstract

The present application relates to a kind of double-layer microcapsule biological soil conditioner and its preparation method, its main component is composite bacteria, organic phosphorus degradation enzyme and plant extract microcapsule, three kinds of effective components are included in slow-release film, wherein plant extract microcapsule is wrapped in outer slow-release film to form double-layer microcapsule structure, can sustainably release conditioner, thereby extend the action time of biological soil conditioner, and, the component in plant extract can promote bacteria growth and produce soil conditioning beneficial biological enzyme, both can take effect quickly and maintain long-term effective conditioning effect, avoid frequent multiple application, reduce the cost and workload of soil conditioning.The preparation method of the double-layer microcapsule biological soil conditioner of the present application is simple and convenient, and the prepared microcapsule has good slow-release performance, can sustainably and slowly release effective components, extend the use time of soil conditioner, reduce the use frequency, and reduce the cost of soil conditioning.

Description

Technical Field

[0001] This invention relates to the field of soil conditioner technology, and in particular to a double-layer microcapsule bioenzyme conditioner and its preparation method. Background Technology

[0002] In recent years, improper application methods, habits, and amounts of chemical fertilizers have led to serious problems such as deterioration of soil physical and chemical properties, gradual decline in soil fertility, soil compaction, and acidification. To address the issues of high pesticide residues, soil compaction, and the presence of toxic and harmful organic matter in the soil, existing technologies utilize microorganisms for soil remediation. For example, Chinese patent document CN103305497B discloses a microcapsule for immobilized enzyme remediation of organically polluted soil and its preparation method; Chinese patent document CN111607404A discloses a method for preparing a compound bacterial soil conditioner and its product; and Chinese patent document CN106190883A discloses a soil-improving compound bacterial composition and its preparation method. These existing technologies play a role in soil remediation to some extent. However, existing biological soil conditioners generally have short effective periods, require repeated replenishment, and are consumed in large quantities while exhibiting slow remediation speeds.

[0003] Therefore, in view of the problems existing in the current technology, there is an urgent need to provide a biological soil conditioning technology that has a good, fast and long-lasting effect on soil conditioning and restoration. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a biological soil conditioning double-layer microcapsule that has a good, fast and long-lasting effect on soil conditioning and restoration.

[0005] The objective of this invention is achieved through the following technical solution:

[0006] A dual-layer microcapsule bio-soil conditioner is provided, comprising an outer slow-release membrane, the outer slow-release membrane containing a substrate, compound bacteria, organophosphorus degrading enzymes, and plant extract microcapsules, calculated by weight.

[0007] The base material includes 10-25 parts glucose, 10-20 parts dolomite powder, and 50-70 parts sodium alginate;

[0008] The compound bacteria include 2-5 parts of powdered Pichia pastoris, 2-5 parts of Dirk Saccharomyces cerevisiae, 2-5 parts of Pediococcus lactis, and 3-6 parts of EM bacteria;

[0009] The content of organophosphorus degrading enzyme is 1 to 3 parts;

[0010] The plant extract microcapsules consist of a sustained-release core membrane and 2 to 5 parts of plant extracts encapsulated within the sustained-release core membrane. The plant extracts include Astragalus membranaceus extract and Citrus reticulata extract.

[0011] Preferably, the ratio of Astragalus extract to tangerine peel extract is 1:1.

[0012] Preferably, by weight, the substrate comprises 10 parts glucose, 10 parts dolomite powder, and 50 parts sodium alginate; the compound bacteria comprises 2 parts powdered Pichia pastoris, 2 parts Dioscorea opposita, 2 parts Pediococcus lactis, and 3 parts EM bacteria; the content of the organophosphorus degrading enzyme is 1 part; and 2 parts plant extract microcapsules.

[0013] In another preferred embodiment, the substrate comprises, by weight, 25 parts glucose, 25 parts dolomite powder, and 70 parts sodium alginate; the compound bacteria comprise 5 parts powdered Pichia pastoris, 5 parts Dioscorea glabra, 5 parts Pediococcus lactis, and 6 parts EM bacteria; the content of the organophosphorus degrading enzyme is 3 parts; and 5 parts plant extract microcapsules.

[0014] In another preferred embodiment, the substrate comprises, by weight, 18 parts glucose, 17 parts dolomite powder, and 60 parts sodium alginate; the compound bacteria comprise 3 parts powdered Pichia pastoris, 3 parts Dioscorea opposita, 3 parts Pediococcus lactis, and 4 parts EM bacteria; the content of the organophosphorus degrading enzyme is 2 parts; and the content of plant extract microcapsules is 3 parts.

[0015] Preferably, each double-layer microcapsule weighs 1-5g, and the total number of complex bacteria in each double-layer microcapsule exceeds 1*102. 7 cfu / g.

[0016] Another objective of this invention is to overcome the shortcomings of the prior art and provide a biological soil conditioning bilayer microcapsule that is fast and long-lasting in soil conditioning and restoration.

[0017] Another objective of this invention is achieved through the following technical solution:

[0018] A method for preparing a double-layer microcapsule bio-soil conditioner is provided, comprising the following main steps:

[0019] (1) Preparation of plant extract microcapsules

[0020] 1.1 Dissolve chitosan in 1% acetic acid solution and stir until homogeneous. Then add astragalus extract and tangerine peel extract and stir at 35~40℃ until completely dissolved to obtain chitosan plant extract solution for later use.

[0021] 1.2 Place liquid paraffin and petroleum ether in a 3:1 volume ratio in a reactor, add emulsifier and stir together for 10-15 minutes;

[0022] 1.3 Slowly add the chitosan plant extract solution to the reactor in 1.2 and disperse it by ultrasonication at a constant temperature of 38~45℃;

[0023] 1.4 Add the crosslinking agent to step 1.3 and stir at a constant temperature for 2-4 hours;

[0024] 1.5 Centrifuge the reaction product from step 1.4, collect the precipitate, and wash it with petroleum ether repeatedly until the supernatant is clear.

[0025] 1.6 The product cleaned in step 1.5 was filtered to obtain plant extract microcapsules containing Astragalus membranaceus extract and Citrus reticulata extract;

[0026] (2) Fermentation by compound bacteria culture

[0027] 2.1 Place the compound microbial community into a fermentation tank, then add rice bran, soybean flour, milk, plant extract microcapsules and water to the fermentation tank, and incubate for 3-7 days.

[0028] (3) Preparation of soil conditioner

[0029] 3.1 Add sodium alginate powder to ultrapure water and stir with ultrasonic assistance. The mass ratio of sodium alginate to water is 1:50.

[0030] 3.2 Add the cultured compound bacteria, organophosphorus degrading enzyme, glucose, dolomite powder and plant extract microcapsules to the gel, stir and mix, pour the mixture into a mold and dry at 40~50℃.

[0031] Preferably, the emulsifier accounts for 0.5% to 10% of the weight of liquid paraffin and petroleum ether.

[0032] Preferably, the emulsifier accounts for 5% of the weight of the liquid paraffin and petroleum ether.

[0033] Preferably, the viscosity of the sodium alginate is ≥2000 cP.

[0034] The beneficial effects of this invention are:

[0035] (1) The main components of the double-layer microcapsule biological soil conditioner of the present invention are compound bacteria, organophosphorus degrading enzymes and plant extract microcapsules. The three effective components are included in the slow-release membrane. The plant extract microcapsules are wrapped in the outer slow-release membrane to form a double-layer microcapsule structure, which can release the conditioner for a long time, thereby prolonging the action time of the biological soil conditioner. Moreover, the components in the plant extract can promote the growth of bacteria and produce biological enzymes that are beneficial to soil conditioning. It can take effect quickly and maintain a long-term effective conditioning effect, avoiding frequent and repeated application, and reducing the cost and workload of soil conditioning.

[0036] (2) The compound bacteria of the present invention include powdered Pichia pastoris, Dirk's yeast, Pediococcus lactis, and EM bacteria. The overall compound bacteria have the characteristics of strong vitality and long-term activity, and can carry out long-lasting and effective biological conditioning of the soil. EM bacteria are composed of about 80 kinds of microorganisms and were developed by Professor Teruo Higa of the University of the Ryukyus in Japan. This bacterial agent can quickly and stably occupy the ecological position in the soil, form a dominant community of beneficial microorganisms, which is beneficial to plant growth and also has a certain conditioning effect on the soil. Through research, the present invention adds three bacteria, Pediococcus lactis, Pichia pastoris, and Dirk's yeast, to EM bacteria to produce a synergistic effect. The resulting compound bacteria not only have a nutrient-enhancing effect on barren soil, but also, when combined with organophosphorus degrading enzymes, have a beneficial effect on reducing the nitrogen, phosphorus, and potassium content of soil with excessive nitrogen, phosphorus, and potassium content, and stabilize the nitrogen, phosphorus, and potassium content of the soil within the normal range, and can effectively reduce the content of polycyclic aromatic hydrocarbons in the soil. Among them, Pichia pastoris can coordinate fermentation with lactic acid bacteria and EM bacteria, mutually promoting fermentation ability to transform harmful substances into harmless substances and condition soil organic matter. At the same time, Pichia pastoris stimulates the proliferation and growth of other bacteria in the compound bacteria, prolonging the overall biological activity of the conditioner. Dioscorea opposita is often used in brewing to enhance aroma, but has few other uses. Research has shown that the compound bacteria made by this invention with Pediococcus lactis, Pichia pastoris, and EM bacteria, combined with the plant extracts of this invention, have very outstanding effects. Dioscorea opposita itself does not have the function of fermentation or direct soil conditioning, but it can reproduce and produce well by utilizing the substances slowly released from the plant microcapsules of this invention. At the same time, when mixed with Pediococcus lactis, it can activate the activity of acidic protease, enhancing the ability of Pediococcus lactis to decompose organic matter and condition soil. Moreover, Dioscorea opposita and its metabolites have a antagonistic effect on pathogenic microorganisms in the soil, which may inhibit harmful pathogenic microorganisms and keep the soil in a "healthy" state.

[0037] (3) The plant extract microcapsules of the present invention contain Astragalus membranaceus extract and Citrus reticulata extract. Astragalus membranaceus extract mainly contains saponins, flavonoids, polysaccharides, and amino acids, while Citrus reticulata extract contains polysaccharides, amino acids, various small molecule alkenes and alcohols, and limonoids. Among them, polysaccharides and amino acids can provide rich nutrients for the compound bacteria, promote the growth, reproduction and metabolism of the compound bacteria, and prolong the service life of the overall conditioner. Saponins have the function of surfactants due to the different degrees of lipophilicity of aglycones and the strong hydrophilicity of sugar chains. In the soil, they can loosen the soil structure, promote the fermentation of soil by compound bacteria to produce small molecule organic acids, accelerate the soil conditioning and recovery speed and improve the soil compaction. At the same time, Astragalus membranaceus extract has the function of hygroscopic and water-locking, which is conducive to the growth of microorganisms and creates a good living environment for the biological community. The alcohols in Citrus reticulata extract can capture organic pollutants (such as polycyclic aromatic hydrocarbon pollutants) and then treat the organic pollutants by compound bacteria and degradation enzymes. The oxidation of alcohols to produce organic acids makes the soil acidified. At the same time, Astragalus membranaceus extract also contains alkaloids such as choline and betaine, which together create an environment with a pH value suitable for the growth and reproduction of microorganisms. Astragalus extract and tangerine peel extract are encapsulated in a slow-release membrane for slow and sustained release, providing long-lasting beneficial effects. Furthermore, the olefins and alcohols in the tangerine peel extract are volatile and can attract small soil animals (earthworms, ants, and springtails, etc.) and promote their digestion, thus conditioning the soil through the growth, digestion, and activity of these animals.

[0038] (4) The present invention provides the stimulating substances required for microbial fermentation through plant extract microcapsules, which can significantly increase the concentration level of microbial preparations. By adding specific microcapsule-encapsulated microbial preparations to soil improvement, the present invention can target specific pollution in the environment. Microbial enzymes can efficiently decompose organic pollutants and produce carbon dioxide and water that are harmless to the environment, thereby improving the environment of this system.

[0039] (5) The preparation method of the double-layer microcapsule biological soil conditioner of the present invention is simple and convenient, and the prepared microcapsules have good slow-release performance, which can release the effective ingredients slowly and for a long time, extend the service time of the soil conditioner, reduce the number of times it is used, and reduce the cost of soil conditioning. Detailed Implementation

[0040] The present invention will be further described in conjunction with the following embodiments. Example 1

[0041] The double-layer microcapsule bio-soil conditioner of this embodiment includes an outer slow-release membrane, which contains a substrate, compound bacteria, organophosphorus degrading enzymes, and plant extract microcapsules, calculated according to their mass fraction relative to the total mass of the microcapsules:

[0042] The base material includes 10 kg of glucose, 10 kg of dolomite powder, and 70 kg of sodium alginate.

[0043] The compound bacteria include 2 kg of powdered Pichia pastoris, 2 kg of Dirk Saccharomyces cerevisiae, 2 kg of Pediococcus lactis, and 3 kg of EM bacteria.

[0044] The content of organophosphorus degrading enzyme is 1 kg;

[0045] The plant extract microcapsule comprises a sustained-release core membrane and 2 kg of plant extract encapsulated within the sustained-release core membrane. The plant extract includes Astragalus membranaceus extract and Citrus reticulata extract. In this embodiment, the ratio of Astragalus membranaceus extract to Citrus reticulata extract is 1:1.

[0046] Each double-layer microcapsule weighs 1-5g, and the total number of complex bacteria in each double-layer microcapsule exceeds 1*102. 7 cfu / g.

[0047] The preparation method of the double-layer microcapsule bio-soil conditioner in this embodiment includes the following main steps:

[0048] (1) Preparation of plant extract microcapsules

[0049] 1.1 Dissolve chitosan in 1% acetic acid solution and stir until homogeneous. Then add astragalus extract and tangerine peel extract and stir at 35°C until completely dissolved to obtain chitosan plant extract solution for later use.

[0050] 1.2 Place liquid paraffin and petroleum ether in a 3:1 volume ratio in a reactor, add emulsifier and stir together for 10 min; the emulsifier accounts for 0.5% of the weight of liquid paraffin and petroleum ether.

[0051] 1.3 Slowly add the chitosan plant extract solution to the reactor in 1.2 and disperse it by ultrasonication at a constant temperature of 38°C;

[0052] 1.4 Add the crosslinking agent to step 1.3 and stir at a constant temperature for 2 hours;

[0053] 1.5 Centrifuge the reaction product from step 1.4, collect the precipitate, and wash it with petroleum ether repeatedly until the supernatant is clear.

[0054] 1.6 The product cleaned in step 1.5 was filtered to obtain plant extract microcapsules containing Astragalus membranaceus extract and Citrus reticulata extract;

[0055] (2) Fermentation of compound bacteria

[0056] 2.1 Place the compound microbial community into a fermentation tank, then add rice bran, soybean powder, milk, plant extract microcapsules and water to the fermentation tank, and incubate for 3 days.

[0057] (3) Preparation of soil conditioner

[0058] 3.1 Add sodium alginate powder to ultrapure water and stir with ultrasonic assistance. The mass ratio of sodium alginate to water is 1:50. The viscosity of sodium alginate is ≥2,000 cP.

[0059] 3.2 Add the cultured compound bacteria, organophosphorus degrading enzyme, glucose, dolomite powder and plant extract microcapsules to the gel, stir and mix, pour the mixture into a mold and dry at 40°C.

[0060] It should be noted that all of the above raw materials can be purchased from the market. Example 2

[0061] The main technical solution of this embodiment is basically the same as that of Embodiment 1. Features not explained in this embodiment are explained using the methods in Embodiment 1, and will not be repeated here. The difference between the double-layer microcapsule bio-soil conditioner of this embodiment and that of Embodiment 1 is as follows:

[0062] The base material includes 25 kg of glucose, 20 kg of dolomite powder, and 50 kg of sodium alginate;

[0063] The compound bacteria consist of 5 kg of powdered Pichia pastoris, 5 kg of Dirk Saccharomyces cerevisiae, 5 kg of Pietrocarpus lactis, and 4 kg of EM bacteria.

[0064] The content of organophosphorus degrading enzyme is 3 kg;

[0065] The plant extract microcapsules consist of a sustained-release core membrane and 5 kg of plant extracts encapsulated within the sustained-release core membrane. The plant extracts include Astragalus membranaceus extract and Citrus reticulata extract, with the ratio of Astragalus membranaceus extract to Citrus reticulata extract being 1:1.

[0066] The difference between the preparation method of the double-layer microcapsule bio-soil conditioner in this embodiment and that in Example 1 is:

[0067] 1.1 Stir at 40°C until completely dissolved;

[0068] 1.2 Stir for 15 minutes, the emulsifier should account for 10% of the weight of liquid paraffin and petroleum ether;

[0069] 1.3 Isothermal ultrasonic dispersion at 45℃;

[0070] 1.4 Stir the reaction for 4 hours;

[0071] 2.1 Incubate and ferment for 7 days;

[0072] 3.2 Dry at 50°C. Example 3

[0073] The main technical solution of this embodiment is basically the same as that of Embodiment 1 or Embodiment 2. Features not explained in this embodiment are explained using the methods in Embodiment 1 or Embodiment 2, and will not be repeated here. The difference between the double-layer microcapsule bio-soil conditioner of this embodiment and that of Embodiment 1 is as follows:

[0074] The base material includes 18 kg of glucose, 17 kg of dolomite powder, and 60 kg of sodium alginate.

[0075] The compound bacteria include 3 kg of powdered Pichia pastoris, 3 kg of Dirk Saccharomyces cerevisiae, 3 kg of Pietrocarpus lactis, and 6 kg of EM bacteria.

[0076] The content of organophosphorus degrading enzyme is 2 kg;

[0077] 3kg of plant extracts.

[0078] The difference between the preparation method of the double-layer microcapsule bio-soil conditioner in this embodiment and that in Example 1 is:

[0079] 1.1 Stir at 38°C until completely dissolved;

[0080] 1.2 Stir for 12 minutes, the emulsifier should account for 5% of the weight of liquid paraffin and petroleum ether;

[0081] 1.3 Isothermal ultrasonic dispersion at 40℃;

[0082] 1.4 Stir and react for 3 hours;

[0083] 2.1 Incubate and ferment for 5 days;

[0084] 3.2 Dry at 45℃.

[0085] Comparative Test 1: Test of Slow-Release Compound Bacteria Content

[0086] Accurately weigh 20 mg of the bilayer microcapsule bio-soil conditioner prepared in Examples 1, 2, and 3, dissolve it in 40 mL of PBS buffer with a pH of 6.8, and conduct dissolution experiments at 37°C and 150 r / min. At set time points (0.5 h, 1 h, 3 h, 6 h, 12 h, 24 h, 72 h, 120 h), take 5 mL samples, centrifuge at 4000 r / min for 5 min, and measure the absorbance of the supernatant to calculate the concentration of the composite bacteria. When measuring the OD value on a spectrophotometer, a blank control containing the same amounts of Astragalus membranaceus extract and Citrus reticulata extract as in Examples 1-3, with a pH of 6.8, was used for zeroing. That is, for all tests of Examples 1-3, a blank control was prepared before zeroing before measuring the absorbance of the composite bacteria in the tested solution. To compare the effects of Astragalus membranaceus extract and Citrus reticulata extract on the composite bacteria, an additional control experiment was added. The bilayer microcapsule bio-soil conditioner in the control experiment was identical in materials and operation methods except that no plant extract microcapsules were added. The test data (concentration of the compound bacteria is in CFU / mL) are as follows:

[0087] Table 1

[0088]

[0089] After the above four experiments were completed, 5 mL of suspension was taken from each experiment, the microcapsules were separated by centrifugation, 5 mL of fresh PBS was added, and the microcapsule structure was destroyed by sonication. The treated suspension was then centrifuged at 4000 r / min for 5 min, and the absorbance value of the supernatant was measured to calculate the percentage of undissolved active ingredients (unreleased content, weight percentage). The test data are as follows:

[0090] Table 2

[0091]

[0092] As can be seen from Table 1, the soil conditioner with added plant extract microcapsules can promote the production and reproduction of compound bacteria, so that the number of compound bacteria during use is greater than the number at the time of input. Therefore, it can be inferred that the soil conditioner of the present invention can condition the soil for a long time.

[0093] As shown in Table 2, after 120 hours, the double-layer microcapsule bio-soil conditioner prepared using the method of this invention still retains more than 30% of its original content, demonstrating excellent slow-release performance.

[0094] Comparative Test 2: Soil Residual Pesticide Conditioning Test

[0095] Test method:

[0096] (1) Prepare 7 soil samples with residual pesticides, each soil sample weighing 100g, and put the soil into a culture box. Maintain the same constant temperature, humidity and ventilation conditions during the experiment.

[0097] (2) Prepare 5 portions of conditioning agent, namely conditioning agent No. 1 to No. 5, each portion of conditioning agent weighing 3g;

[0098] Conditioner No. 1: The bio-soil conditioner prepared in Example 2;

[0099] Conditioner #2: Except for not adding Pichia pastoris, it is the same as Conditioner #1. The amount of Pichia pastoris that is missing is replaced with an equal amount of Dioscorea deliciosa, Pediococcus lactis and EM bacteria in a 1:1:1 ratio.

[0100] Conditioner #3: Except for not adding Dioscorea yeast, it is the same as Conditioner #1. The amount of Dioscorea yeast that is less is replaced with an equal amount of Pichia pastoris, Pediococcus lactis and EM bacteria in a 1:1:1 ratio.

[0101] Conditioner #4: Except for the absence of Pediococcus lactis, it is the same as Conditioner #1. The amount of Pediococcus lactis that is missing is replaced with an equal amount of Dioscorea opposita, Pichia pastoris and EM bacteria in a 1:1:1 ratio.

[0102] Conditioner #5: Same as Conditioner #1 except that it does not contain plant extract microcapsules;

[0103] Conditioner #6: Except for not adding EM bacteria, it is the same as Conditioner #1. The amount of EM bacteria that is less is replaced with an equal amount of Dioscorea opposita, Pichia pastoris and Pediococcus lactis in a 1:1:1 ratio.

[0104] (3) Dissolve 5 portions of conditioner in 20 mL of water to prepare a single conditioner solution, and spray each solution onto 1 soil sample. Keep one blank soil sample as a control. Begin soil conditioning for 3 months. Spray the conditioner solution every 10 days. Take samples for testing on the 92nd day. The weight of each test sample is 10 g.

[0105] (4) Select several specific substances and measure the changes in their content.

[0106] 4.1 Determination of total HCH in soil, the determination method is as specified in GB / T 14550 Soil Quality - Determination of HCH and DDT - Gas Chromatography

[0107] 4.2 Determination of total DDT in soil, the determination method refers to "GB / T 14550 Soil Quality - Determination of DDT and DDT by Gas Chromatography";

[0108] 4.3 Determination of total polycyclic aromatic hydrocarbons (PAHs) in soil. The determination method was in accordance with ISO 13859-2014 Soil quality. The determination of PAHs was performed by gas chromatography-mass spectrometry (GC) and high-performance liquid chromatography (HPLC).

[0109] The test data (content unit is mg / kg) are as follows:

[0110] Table 3

[0111]

[0112] As shown in Table 3, this invention effectively reduces the content of residual pesticides and polycyclic aromatic hydrocarbons (PAHs) in the soil. Furthermore, the effect of compound bacteria No. 1 is significantly better than the others. This indicates that the overall effect of the compound bacteria in conditioning the soil is better than that of other mixed bacteria. This is because the specific bacteria in this invention can produce a synergistic effect greater than the sum of its parts (1+1>2), demonstrating significant progress. In this experiment, although products No. 3 and No. 5 were slightly better at removing PAHs than No. 6, they were much less effective than other conditioners. This suggests that *Dacron* has a good effect on removing PAHs. Similarly, the fact that conditioner No. 5, which does not contain plant extract microcapsules, is less effective than products No. 1 to No. 4 suggests that the plant extract microcapsules themselves have the function of eliminating PAHs, or that they can promote the removal of organic pesticide residues and PAHs by the compound bacteria. Alternatively, some components in the plant extract microcapsules can react with organic pollutants under the action of the compound bacteria (the specific mechanism is not yet clear). Although the mechanism by which the components in the plant extract microcapsules react with various bacteria to eliminate toxic pollutants is not yet clear, it does not affect the overall beneficial effect.

[0113] Comparative Test 3: Soil Conditioning Test with Lower Nitrogen, Phosphorus, and Potassium Content

[0114] Test method:

[0115] (1) Prepare 7 soil samples with low nitrogen, phosphorus and potassium content collected from the same place for later use. Each soil sample weighs 100g. Put the soil into a culture box and maintain the same constant temperature, humidity and ventilation conditions during the experiment.

[0116] (2) Prepare 5 portions of conditioning agent, namely conditioning agent No. 1 to No. 5, each portion of conditioning agent weighing 3g;

[0117] Conditioner No. 1: The bio-soil conditioner prepared in Example 2;

[0118] Conditioner #2: Except for not adding Pichia pastoris, it is the same as Conditioner #1. The amount of Pichia pastoris that is missing is replaced with an equal amount of Dioscorea deliciosa, Pediococcus lactis and EM bacteria in a 1:1:1 ratio.

[0119] Conditioner #3: Except for not adding Dioscorea yeast, it is the same as Conditioner #1. The amount of Dioscorea yeast that is less is replaced with an equal amount of Pichia pastoris, Pediococcus lactis and EM bacteria in a 1:1:1 ratio.

[0120] Conditioner #4: Except for the absence of Pediococcus lactis, it is the same as Conditioner #1. The amount of Pediococcus lactis that is missing is replaced with an equal amount of Dioscorea opposita, Pichia pastoris and EM bacteria in a 1:1:1 ratio.

[0121] Conditioner #5: Same as Conditioner #1 except that it does not contain plant extract microcapsules;

[0122] Conditioner #6: Except for not adding EM bacteria, it is the same as Conditioner #1. The amount of EM bacteria that is less is replaced with an equal amount of Dioscorea opposita, Pichia pastoris and Pediococcus lactis in a 1:1:1 ratio.

[0123] (3) Dissolve 5 portions of conditioner in 20 mL of water to prepare a single conditioner solution, and spray each solution onto 1 soil sample. Keep one blank soil sample as a control. Begin soil conditioning for 3 months. Spray the conditioner solution every 10 days. Take samples for testing on the 92nd day. The weight of each test sample is 10 g.

[0124] (4) Determination of the content of specific substances

[0125] 4.1 Determination of alkaline nitrogen;

[0126] 4.2 Determination of available phosphorus;

[0127] 4.3 Determination of available potassium;

[0128] 4.1 to 4.3 were all tested using a soil testing instrument. The test data (content unit is mg / kg) are as follows:

[0129] Table 4

[0130]

[0131] Analysis of the data in Table 4 shows that after only 3 months of conditioning, the content of effectively absorbable nitrogen, phosphorus, and potassium all increased to some extent. This indicates that the double-layer microcapsules of the soil conditioner of this invention have a soil fertility restoration effect. In actual conditioning, for nutrient-poor soils, the double-layer microcapsules of the soil conditioner of this invention can also be used in conjunction with organic fertilizers such as manure to achieve rapid conditioning and long-lasting fertility. Table 4 also shows that conditioner No. 6, which lacks EM bacteria, has the worst effect on soil eutrophication, followed by Pediococcus lactis. This suggests that EM bacteria and Pediococcus lactis are the main components for conditioning soil nutrient levels, with other components providing auxiliary and synergistic effects. Conditioner No. 1 has the best conditioning effect compared to other conditioners, indicating that the compound bacteria are more effective than other components, and that the bacterial species produce a beneficial synergistic effect of mutual promotion. Conditioner No. 2, No. 3, and No. 4 can also increase the content of effective nitrogen, phosphorus, and potassium. Among them, conditioner No. 3 has a better conditioning effect than No. 2, while conditioners No. 4 and No. 6 have a poorer conditioning effect. Comparative analysis shows that the compound bacteria with plant extract microcapsules have the best soil conditioning effect. Pichia pastoris and Dioscorea deliciosa can work synergistically with Pediococcus lactis and EM bacteria to achieve better conditioning effect than single bacteria or two bacteria.

[0132] The effect of treatment No. 5 was less than that of No. 1, suggesting that the astragalus extract and tangerine peel extract in the plant extract microcapsules can also promote the metabolism of the compound bacteria to improve the conditioning ability. Furthermore, in the experiment, supplementing the conditioning agent at 10-day intervals still resulted in excellent soil restoration effects, indirectly indicating that the double-layer microcapsule structure of this invention has good sustained-release properties and can maintain its effectiveness for a long time.

[0133] Comparative Test 4: Soil pH Test

[0134] (1) Prepare 4 soil samples collected from the same location for later use, each soil sample weighing 100g;

[0135] (2) Take one soil sample as a control sample and number it No. 1; mix 5g of the double-layer microcapsule biological soil conditioner of Example 1 into three soil samples respectively and number them No. 2, No. 3 and No. 4 respectively.

[0136] (3) Place 4 soil samples in the same incubator and keep the temperature at 22~28℃. Spray distilled water and turn the soil over every 24 hours. The amount of water should be enough to moisten the soil. Continue this incubation until the 30th day.

[0137] (4) Take 10g of soil from each of the four small beakers and add 25mL of distilled water to each beaker and stir thoroughly.

[0138] (5) Calibrate the pH meter;

[0139] (6) Insert the pH meter into the solutions in the four small beakers to test the pH values ​​of solutions 1 to 4. Before each measurement of a different solution, clean the electrodes of the pH meter with distilled water.

[0140] The test data is as follows:

[0141] Table 5

[0142]

[0143] As shown in Table 5, after 30 days of conditioning, the pH of the soil decreased to a certain extent, which indicates that the product of this invention can solve the problem of soil compaction.

[0144] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the claims. Those skilled in the art should understand, with reference to the preferred embodiments, that modifications or equivalent substitutions can be made to the technical solutions of the present invention, but such modifications or substitutions shall fall within the substantially the same scope of protection as the technical solutions of the present invention.

Claims

1. A double-layered microcapsule biosoil conditioner, characterized by, It comprises an outer slow-release film, and the outer slow-release film contains a base material, compound bacteria, organic phosphorus degradation enzyme and plant extract microcapsules, and the base material, compound bacteria, organic phosphorus degradation enzyme and plant extract microcapsules are calculated according to weight parts, The base material comprises 10-25 parts of glucose, 10-20 parts of dolomite powder and 50-70 parts of sodium alginate; The compound bacteria comprise 2-5 parts of powdered Pichia pastoris, 2-5 parts of Dekker yeast, 2-5 parts of lactococcus lactis and 3-6 parts of EM bacteria; The content of the organic phosphorus degradation enzyme is 1-3 parts; The plant extract microcapsules comprise 2-5 parts of plant extract microcapsules, and the plant extract microcapsules comprise a slow-release core membrane and plant extract wrapped in the slow-release core membrane; the plant extract comprises Astragalus extract and dried orange peel extract, and the content ratio of the Astragalus extract and the dried orange peel extract is 1:

1.

2. The double-layered microcapsule biosoil conditioner according to claim 1, characterized in that, According to weight parts, The base material comprises 10 parts of glucose, 10 parts of dolomite powder and 50 parts of sodium alginate; The compound bacteria comprise 2 parts of powdered Pichia pastoris, 2 parts of Dekker yeast, 2 parts of lactococcus lactis and 3 parts of EM bacteria; The content of the organic phosphorus degradation enzyme is 1 part; The plant extract microcapsules are 2 parts.

3. The double-layered microcapsule biosoil conditioner according to claim 1, characterized in that, According to weight parts, The base material comprises 25 parts of glucose, 25 parts of dolomite powder and 70 parts of sodium alginate; The compound bacteria comprise 5 parts of powdered Pichia pastoris, 5 parts of Dekker yeast, 5 parts of lactococcus lactis and 6 parts of EM bacteria; The content of the organic phosphorus degradation enzyme is 3 parts; The plant extract microcapsules are 5 parts.

4. The double-layer microcapsule biosoil conditioner according to claim 1, characterized in that, According to weight parts, The base material comprises 18 parts of glucose, 17 parts of dolomite powder and 60 parts of sodium alginate; The compound bacteria comprise 3 parts of powdered Pichia pastoris, 3 parts of Dekker yeast, 3 parts of lactococcus lactis and 4 parts of EM bacteria; The content of the organic phosphorus degradation enzyme is 2 parts; The plant extract microcapsules are 3 parts.

5. The double-layer microcapsule biosoil conditioner according to claim 1, characterized in that, Each double-layer microcapsule weighs 1-5 g, and the total amount of complex bacteria in each double-layer microcapsule exceeds 1*10 7 cfu / g.

6. A method for preparing a double-layer microcapsule bio-soil conditioner, characterized by, The method comprises the following main steps: (1) Preparation of plant extract microcapsules 1.1 Dissolve chitosan in 1% acetic acid solution and stir uniformly, then add Astragalus extract and dried orange peel extract, and stir until completely dissolved at a temperature of 35-40°C to prepare a chitosan plant extract solution, wherein the content ratio of the Astragalus extract and the dried orange peel extract is 1:1; 1.2 Put liquid paraffin and petroleum ether with a volume ratio of 3:1 into a reactor, and add an emulsifier to stir together for 10-15 min; 1.3 Slowly add the chitosan plant extract solution to the reactor of 1.2, and ultrasonically disperse at a constant temperature of 38-45°C; 1.4 Add a crosslinking agent to the step 1.3 and stir to react at a constant temperature for 2-4 h; 1.5 Centrifuge the reaction product of step 1.4 to collect the precipitate, and wash with petroleum ether repeatedly until the supernatant is clear; 1.6 Extract the washed product of step 1.5 by suction filtration to obtain plant extract microcapsules containing Astragalus extract and dried orange peel extract; (2) Cultivation and fermentation of compound bacteria 2.1 Put the compound bacteria into a fermentation tank, and put rice bran, soybean powder, milk, plant extract microcapsules and water into the fermentation tank, and incubate and ferment for 3-7 days; (3) Preparation of soil conditioner 3.1 Add sodium alginate powder into ultrapure water, and stir under ultrasonic assistance, wherein the mass ratio of sodium alginate to water is 1:50; 3.2 Add the cultured compound bacteria, organophosphorus-degrading enzyme, glucose, dolomite powder and plant extract microcapsules into the gel, stir and mix, pour the mixed solution into a mold, and dry at 40-50 DEG C to obtain a double-layer microcapsule structure biological soil conditioner.

7. The method of claim 6, wherein the biologically active material is selected from the group consisting of micronutrients, fertilizers, pesticides, herbicides, and combinations thereof. The emulsifier accounts for 0.5%-10% of the weight of the liquid paraffin and petroleum ether.

8. The method of claim 7, wherein the method is characterized by: The emulsifier accounts for 5% of the weight of the liquid paraffin and petroleum ether.

9. The process for preparing a double-layered microcapsule biosoil conditioner according to claim 6, characterized by, The viscosity of the sodium alginate is greater than or equal to 2,000 cP. The viscosity of the sodium alginate is greater than or equal to 2,000 cP.

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