A lipid plant nutrient composition and its preparation method and application

Abstract

The present invention provides a lipid phytonutrient composition and a preparation method and application thereof. The lipid phytonutrient composition comprises natural vitamin E, plant squalene, plant sterols, long carbon chain fatty alkanols, plant waxes or plant hydrocarbons and other components. The present invention performs esterification / ester exchange, multi-stage distillation and saponification extraction, refining and other steps on rice bran oil deodorization distillate to finally obtain the lipid phytonutrient composition. The lipid phytonutrient composition can be applied to multiple fields such as food, feed and cosmetics. Therefore, the present invention extracts a new and useful composition from the rice bran oil deodorization distillate, further expanding the application value of the rice bran oil deodorization distillate.

Description

Technical Field

[0001] The present invention relates to the technical field of lipid compounds, and in particular to a lipid plant nutrient composition, a preparation method and an application thereof. Background Art

[0002] Vegetable oil residue, also known as deodorizer distillate oil (DD oil for short), is a by-product produced during the deodorization stage of edible oil refining. It is mainly composed of fatty acids and neutral oils, and is rich in high-value-added natural active substances such as natural vitamin E, phytosterols and squalene. Deodorization is a major process in vegetable oil processing. The principle of its process technology is to use the vapor pressure difference to separate the various components in the oil from triglycerides. The purpose of this process is to improve the flavor of the oil and improve the storage stability of the oil. Vegetable oil deodorizer distillate is the only source of raw materials for the natural vitamin E, phytosterols and squalene industries at home and abroad, and is irreplaceable.

[0003] Natural vitamin E (VE), also known as anti-fertility vitamin, is a general term for tocopherols, tocotrienols and derivatives with d-α-tocopherol activity. It is widely found in animal and plant fats, organs, vegetable oils and chlorophyll. According to the position and number of methyl groups on the chromone ring, it is divided into 8 compounds, namely 4 tocopherols and 4 tocotrienols (α-, β-, γ- and δ-types). Natural vitamin E is a natural antioxidant, and its antioxidant effect works both in vivo and in vitro. At present, the demand for natural vitamin E in the health care, medicine, food, cosmetics and feed industries is growing steadily. With the increasing interest in natural products, natural vitamin E is becoming more and more popular.

[0004] Phytosterols have the advantages of good physiological functions and high safety. They can be widely used in the fields of medicine, food, feed, cosmetics, etc., have broad market application prospects, and have been approved by the FDA as "healthy" functional foods and are widely used in the pharmaceutical industry. The history of sterol research is hundreds of years long. With the advancement of science and technology and the deepening of research, people's understanding of the important role and physiological functions of this type of life substance is still deepening. A large number of studies have shown that phytosterols can promote the dissimulation of cholesterol, inhibit the absorption of cholesterol in the intestine and its biosynthesis in the liver, effectively reduce the total cholesterol and LDL-cholesterol levels in serum, have no significant effect on the concentration of HDL-cholesterol and triglycerides, and can prevent and treat cardiovascular diseases.

[0005] Squalene was first discovered in shark liver oil, but due to overfishing of sharks and pressure from animal environmental protection organizations, the animal squalene resources from sharks are facing unsustainable problems. The deodorized distillate after vegetable oil refining contains a large amount of plant squalene, but in the past, due to low extraction technology and high costs, this resource was wasted.

[0006] Long-chain fatty alkanols are generally a mixture of primary fatty alcohols with 22 to 30 carbon atoms. They are widely present in nature as biologically active substances and are natural higher fatty alcohols. In recent years, new uses of long-chain fatty alcohols have been discovered. It is known that C22 alcohol can be used to inhibit prostate tumors; C24 alcohol can enhance the function of nerve factors; C26 alcohol can be used to lower blood lipids; C28 alcohol has the effects of anti-fatigue, lowering blood lipids, and enhancing sexual function. Recently, it has been reported that it can be used to treat early Parkinson's disease in the elderly; C30 alcohol is a recognized plant growth regulator, and also has the effect of promoting animal growth, and can be used to treat skin eczema and herpes.

[0007] In the technology of the present application, rice bran oil deodorized distillate is mainly used as raw material, and active components such as phytosterols, squalene, natural vitamin E and long-chain fatty alkanols are extracted through a certain process. How to develop new utilization value of rice bran oil deodorized distillate and extract new useful compositions from rice bran oil deodorized distillate are technical problems that technicians in this field need to solve. Summary of the invention

[0008] In view of the above situation, the present invention provides a lipid phytonutrient composition and a preparation method and application thereof, so as to extract a new and useful composition from the deodorized distillate of rice bran oil, and further expand the application value of the deodorized distillate of rice bran oil.

[0009] A lipid phytonutrient composition, comprising, by weight percentage:

[0010] 10-30.0wt% natural vitamin E;

[0011] 5-20.0wt% of phytosterols;

[0012] 10-30.0wt% squalene;

[0013] 1-10.0wt% of long carbon chain fatty alkanol;

[0014] The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons;

[0015] Wherein, wt% is based on the total amount of the composition;

[0016] The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

[0017] The iodine value of the lipid phytonutrient composition is 50-150 g / 100 g, the saponification value is less than 3 mgKOH / g, the acid value is less than 1 mgKOH / g, and the peroxide value is less than 5 g / 100 g.

[0018] The lipid phytonutrient composition, wherein the ratio of tocopherol to tocotrienol in the natural vitamin E is:

[0019] Tocopherol: tocotrienol = 0.8-1.2:1.

[0020] The lipid phytonutrient composition, wherein the 4-demethyl sterol in the phytosterol comprises brassicasterol, campesterol, stigmasterol and sitosterol;

[0021] The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

[0022] The above lipid phytonutrient composition, wherein the long carbon chain fatty alkanol comprises C 22 -C 30 Straight chain fatty alkanol.

[0023] A method for preparing a lipid phytonutrient composition comprises the following steps:

[0024] S1, converting fatty acids, glycerides and steroids contained in a deodorized distillate of rice bran oil to obtain lipid phytonutrients containing fatty acid alkyl esters, natural vitamin E, plant squalene, phytosterols and long-chain fatty alkanols;

[0025] S2, cooling the product obtained in step S1, crystallizing and filtering to obtain crude sterol and mother liquor;

[0026] S3, subjecting the mother liquor in step S2 to multi-stage distillation to separate and remove low-boiling-point small-molecule aldehydes, ketones, acids, fatty acid alkyl esters and plant hydrocarbon impurities, and enriching the concentrate of natural vitamin E, phytosterols, plant squalene and long-chain fatty alkanols;

[0027] S4, saponifying, extracting, decolorizing and refining the concentrate in step S3 in an alcohol-alkali solution to obtain the lipid phytonutrient composition.

[0028] The method for preparing the lipid phytonutrient composition, wherein step S1 specifically comprises:

[0029] S1.1, dissolving the rice bran oil deodorization distillate with methanol, dropping concentrated sulfuric acid as a catalyst for methylation, then adding deionized water to terminate the reaction, allowing the layers to stand, removing the lower layer of alcohol, water and sulfuric acid mixed phase, washing the oil phase with hot water until neutral, and dehydrating for standby use;

[0030] S1.2, add a methanol solution of an alkaline catalyst to the reaction solution obtained in step S1.1 to carry out alcoholysis reaction, then add concentrated sulfuric acid to terminate the reaction, add deionized water, let it stand for stratification, remove the mixed phase of methanol, water and sulfuric acid, and recover the methanol in steps S1.1 and S1.2 for reuse, wash the oil phase with hot water again to neutrality, dehydrate and set aside, the oil phase contains the converted lipid phytonutrients including fatty acid methyl esters, natural vitamin E, plant squalene, phytosterols, and long-chain fatty alkanols.

[0031] The method for preparing the lipid phytonutrient composition, wherein step S2 specifically comprises:

[0032] The oil phase after transformation in step S1 is cooled and precipitated. The process is divided into crystallization and crystal growing steps. The oil phase is cooled to 15-20°C at a rate of 1-3°C / h, and then crystals are grown at this temperature for 12-24h.

[0033] In the method for preparing the lipid phytonutrient composition, the multi-stage distillation in step S3 is achieved by:

[0034] The first stage flash evaporation is to add the mother liquor in step S2 to the first stage flash evaporation tower, and the feed temperature is 100-130°C, and the vacuum degree of the tower top system is 200-2000Pa, and the low molecular weight substances are flashed to remove the low molecular weight substances, including water, plant hydrocarbons, fatty acids, aldehydes and ketones;

[0035] The second stage distillation is to continuously add the materials after the first stage flash distillation into the second stage distillation tower, the tower bottom is heated to 160-200°C, the tower top temperature is maintained at 140-180°C, and the vacuum condition is 50-200Pa. The substances removed from the light components in the second stage distillation tower include fatty acid methyl esters, residual natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols;

[0036] Coupled with molecular distillation, the heavy components after the second stage distillation are continuously added into the molecular distillation system, the heating temperature is maintained at 220-240°C, the vacuum condition is 1-10Pa, and the light components after molecular distillation are lipid phytonutrient concentrates, which include natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols, and residual plant asphalt.

[0037] The method for preparing the lipid phytonutrient composition, wherein step S4 specifically comprises:

[0038] S4.1, stirring and neutralizing the lipid phytonutrient concentrate enriched in step S3 with an organic solvent, a low-carbon chain alcohol, deionized water, and a caustic soda at 70-80° C. for 1-2 hours;

[0039] S4.2, subjecting the solution obtained in step S4.1 to static extraction and stratification, and after the stratification interface is clear, stratification is performed, and the upper layer solution is retained for later use;

[0040] S4.3, washing the upper layer solution obtained in step S4.2 with water, allowing it to stand for stratification after washing, and stratifying again after the stratification interface is clear, leaving the upper layer solution for later use;

[0041] S4.4, adding activated carbon to the upper layer solution obtained in step S4.3, stirring to decolorize and purify, then filtering to remove the activated carbon, and using the filtrate for later use;

[0042] S4.5, recovering the organic solvent from the filtrate obtained in step S4.4, and subjecting the filtrate to molecular distillation to obtain the lipid plant nutrient composition;

[0043] S4.6, distilling the lower layer solution obtained in step S4.2, recovering the obtained low-carbon chain alcohol, and applying it to step S1, the distillation condition is normal pressure or reduced pressure.

[0044] The preparation method of the above-mentioned lipid phytonutrient composition, wherein, in the step S4.1, the organic solvent is one of petroleum ether, n-hexane, and n-heptane at 90-120°C, and the addition ratio of the organic solvent to the enriched lipid phytonutrient concentrate is 1-2mL / g.

[0045] The preparation method of the above-mentioned lipid phytonutrient composition, wherein, in the step S4.1, the low-chain alcohol is one of methanol, ethanol, and isopropanol, the addition ratio of the low-chain alcohol to the enriched lipid phytonutrient concentrate is 0.5-2mL / g, and the addition ratio of the deionized water to the enriched lipid phytonutrient concentrate is 0.25-0.5mL / g.

[0046] In the method for preparing the lipid plant nutrient composition, in step S4.1, the caustic soda is sodium hydroxide or potassium hydroxide.

[0047] The present invention also provides the use of the lipid phytonutrient composition in preparing a skin care preparation for human skin.

[0048] The present invention also provides application of the lipid phytonutrient composition in preparing lip balm.

[0049] The present invention also provides application of the lipid plant nutrient composition in preparing feed additives.

[0050] The present invention also provides the use of the lipid phytonutrient composition in preparing a dietary supplement.

[0051] According to the lipid phytonutrient composition provided by the present invention, its preparation method and application, the lipid phytonutrient composition comprises natural vitamin E, plant squalene, phytosterols, long carbon chain fatty alkanols, plant waxes or plant hydrocarbons and other ingredients. The present invention performs esterification / ester exchange, multi-stage distillation and saponification extraction, refining and other steps on the rice bran oil deodorization distillate to finally obtain the lipid phytonutrient composition, and the lipid phytonutrient composition can be applied to multiple fields such as food, feed and cosmetics. Therefore, the present invention extracts a new and useful composition from the rice bran oil deodorization distillate, further expanding the application value of the rice bran oil deodorization distillate. DETAILED DESCRIPTION

[0052] In order to facilitate understanding of the present invention, the present invention will be described more fully below with reference to various embodiments, but the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0053] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which the present invention belongs. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0054] The embodiment of the present invention provides a lipid phytonutrient composition, which comprises, by weight percentage:

[0055] 10-30.0wt% natural vitamin E;

[0056] 5-20.0wt% of phytosterols;

[0057] 10-30.0wt% squalene;

[0058] 1-10.0wt% of long carbon chain fatty alkanol;

[0059] The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons;

[0060] Wherein, wt% is based on the total amount of the composition;

[0061] The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

[0062] The lipid phytonutrient composition has an iodine value of 50-150 g / 100 g, a saponification value of less than 3 mg KOH / g, an acid value of less than 1 mg KOH / g, and a peroxide value of less than 5 g / 100 g.

[0063] The lipid phytonutrient composition, wherein the ratio of tocopherol to tocotrienol in the natural vitamin E is:

[0064] Tocopherol: tocotrienol = 0.8-1.2:1.

[0065] The lipid phytonutrient composition, wherein the 4-desmethyl sterol in the phytosterol comprises brassicasterol, campesterol, stigmasterol and sitosterol;

[0066] The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

[0067] The lipid phytonutrient composition, wherein the long carbon chain fatty alkanol comprises C 22 -C 30 Straight chain fatty alkanol.

[0068] The embodiment of the present invention also provides a method for preparing a lipid phytonutrient composition, comprising the following steps:

[0069] S1, converting fatty acids, glycerides and steroids contained in a deodorized distillate of rice bran oil to obtain lipid phytonutrients containing fatty acid alkyl esters, natural vitamin E, plant squalene, phytosterols and long-chain fatty alkanols;

[0070] S2, cooling the product obtained in step S1, crystallizing and filtering to obtain crude sterol and mother liquor;

[0071] S3, subjecting the mother liquor in step S2 to multi-stage distillation to separate and remove low-boiling-point small-molecule aldehydes, ketones, acids, fatty acid alkyl esters and plant hydrocarbon impurities, and enriching the concentrate of natural vitamin E, phytosterols, plant squalene and long-chain fatty alkanols;

[0072] S4, saponifying, extracting, decolorizing and refining the concentrate in step S3 in an alcohol-alkali solution to obtain the lipid phytonutrient composition.

[0073] The method for preparing the lipid phytonutrient composition, wherein step S1 specifically comprises:

[0074] S1.1, dissolving the rice bran oil deodorization distillate with methanol, dropping concentrated sulfuric acid as a catalyst for methylation, then adding deionized water to terminate the reaction, allowing the layers to stand, removing the lower layer of alcohol, water and sulfuric acid mixed phase, washing the oil phase with hot water until neutral, and dehydrating for standby use;

[0075] S1.2, add a methanol solution of an alkaline catalyst to the reaction solution obtained in step S1.1 to carry out alcoholysis reaction, then add concentrated sulfuric acid to terminate the reaction, add deionized water, let it stand for stratification, remove the mixed phase of methanol, water and sulfuric acid, and recover the methanol in steps S1.1 and S1.2 for reuse, wash the oil phase with hot water again to neutrality, dehydrate and set aside, the oil phase contains the converted lipid phytonutrients including fatty acid methyl esters, natural vitamin E, plant squalene, phytosterols, and long-chain fatty alkanols.

[0076] The method for preparing the lipid phytonutrient composition, wherein step S2 specifically comprises:

[0077] The oil phase after transformation in step S1 is cooled and precipitated. The process is divided into crystallization and crystal growing steps. The oil phase is cooled to 15-20°C at a rate of 1-3°C / h, and then crystals are grown at this temperature for 12-24h.

[0078] In the method for preparing the lipid phytonutrient composition, the multi-stage distillation in step S3 is achieved by:

[0079] The first stage flash evaporation is to add the mother liquor in step S2 to the first stage flash evaporation tower, and the feed temperature is 100-130°C, and the vacuum degree of the tower top system is 200-2000Pa, and the low molecular weight substances are flashed to remove the low molecular weight substances, including water, plant hydrocarbons, fatty acids, aldehydes and ketones;

[0080] The second stage distillation is to continuously add the materials after the first stage flash distillation into the second stage distillation tower, the tower bottom is heated to 160-200°C, the tower top temperature is maintained at 140-180°C, and the vacuum condition is 50-200Pa. The substances removed from the light components in the second stage distillation tower include fatty acid methyl esters, residual natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols;

[0081] Coupled with molecular distillation, the heavy components after the second stage distillation are continuously added into the molecular distillation system, the heating temperature is maintained at 220-240°C, the vacuum condition is 1-10Pa, and the light components after molecular distillation are lipid phytonutrient concentrates, which include natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols, and residual plant asphalt.

[0082] The method for preparing the lipid phytonutrient composition, wherein step S4 specifically comprises:

[0083] S4.1, stirring and neutralizing the lipid phytonutrient concentrate enriched in step S3 with an organic solvent, a low-carbon chain alcohol, deionized water, and a caustic soda at 70-80° C. for 1-2 hours;

[0084] S4.2, subjecting the solution obtained in step S4.1 to static extraction and stratification, and after the stratification interface is clear, stratification is performed, and the upper layer solution is retained for later use;

[0085] S4.3, washing the upper layer solution obtained in step S4.2 with water, allowing it to stand for stratification after washing, and stratifying again after the stratification interface is clear, leaving the upper layer solution for later use;

[0086] S4.4, adding activated carbon to the upper layer solution obtained in step S4.3, stirring to decolorize and purify, then filtering to remove the activated carbon, and using the filtrate for later use;

[0087] S4.5, recovering the organic solvent from the filtrate obtained in step S4.4, and subjecting the filtrate to molecular distillation to obtain the lipid plant nutrient composition;

[0088] S4.6, distilling the lower layer solution obtained in step S4.2, recovering the obtained low-carbon chain alcohol, and applying it to step S1, the distillation condition is normal pressure or reduced pressure.

[0089] The preparation method of the above-mentioned lipid phytonutrient composition, wherein, in the step S4.1, the organic solvent is one of petroleum ether, n-hexane, and n-heptane at 90-120°C, and the addition ratio of the organic solvent to the enriched lipid phytonutrient concentrate is 1-2mL / g.

[0090] The preparation method of the above-mentioned lipid phytonutrient composition, wherein, in the step S4.1, the low-chain alcohol is one of methanol, ethanol, and isopropanol, the addition ratio of the low-chain alcohol to the enriched lipid phytonutrient concentrate is 0.5-2mL / g, and the addition ratio of the deionized water to the enriched lipid phytonutrient concentrate is 0.25-0.5mL / g.

[0091] In the method for preparing the lipid plant nutrient composition, in step S4.1, the caustic soda is sodium hydroxide or potassium hydroxide.

[0092] The following is a further description of the embodiments of the present invention in multiple embodiments. The embodiments of the present invention are not limited to the following specific embodiments. Within the scope of the unchanged main rights, appropriate changes can be made to the implementation.

[0093] Example 1

[0094] A lipid phytonutrient composition, comprising, by weight percentage:

[0095] 20.0wt% natural vitamin E;

[0096] 15.0wt% phytosterols;

[0097] 10.0wt% squalene;

[0098] 4.0wt% of long carbon chain fatty alkanol;

[0099] The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons;

[0100] Wherein, wt% is based on the total amount of the composition;

[0101] The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

[0102] The lipid phytonutrient composition has an iodine value of 50 g / 100 g, a saponification value of 2.5 mgKOH / g, an acid value of 0.7 mgKOH / g, and a peroxide value of 0.2 g / 100 g.

[0103] The lipid phytonutrient composition, wherein the ratio of tocopherol to tocotrienol in the natural vitamin E is:

[0104] Tocopherol: tocotrienol = 0.8:1.

[0105] The lipid phytonutrient composition, wherein the 4-desmethyl sterol in the phytosterol comprises brassicasterol, campesterol, stigmasterol and sitosterol;

[0106] The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

[0107] The lipid phytonutrient composition, wherein the long carbon chain fatty alkanol comprises C 22 -C 30 Straight chain fatty alkanol.

[0108] Example 2

[0109] A lipid phytonutrient composition, comprising, by weight percentage:

[0110] 15.5wt% natural vitamin E;

[0111] 18.0wt% phytosterols;

[0112] 30.0wt% squalene;

[0113] 10.0wt% of long carbon chain fatty alkanol;

[0114] The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons;

[0115] Wherein, wt% is based on the total amount of the composition;

[0116] The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

[0117] The lipid phytonutrient composition has an iodine value of 150 g / 100 g, a saponification value of 2.6 mgKOH / g, an acid value of 0.6 mgKOH / g, and a peroxide value of 0.7 g / 100 g.

[0118] The lipid phytonutrient composition, wherein the ratio of tocopherol to tocotrienol in the natural vitamin E is:

[0119] Tocopherol: tocotrienol = 1:1.

[0120] The lipid phytonutrient composition, wherein the 4-desmethyl sterol in the phytosterol comprises brassicasterol, campesterol, stigmasterol and sitosterol;

[0121] The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

[0122] The lipid phytonutrient composition, wherein the long carbon chain fatty alkanol comprises C 22 -C 30 Straight chain fatty alkanol.

[0123] Example 3

[0124] A lipid phytonutrient composition, comprising, by weight percentage:

[0125] 15.0wt% natural vitamin E;

[0126] 5.0wt% phytosterols;

[0127] 15.0wt% squalene;

[0128] 2.0wt% of long carbon chain fatty alkanol;

[0129] The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons;

[0130] Wherein, wt% is based on the total amount of the composition;

[0131] The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

[0132] The lipid phytonutrient composition has an iodine value of 80 g / 100 g, a saponification value of 1.3 mgKOH / g, an acid value of 0.9 mgKOH / g, and a peroxide value of 0.8 g / 100 g.

[0133] The lipid phytonutrient composition, wherein the ratio of tocopherol to tocotrienol in the natural vitamin E is:

[0134] Tocopherol: tocotrienol = 1.2:1.

[0135] The lipid phytonutrient composition, wherein the 4-desmethyl sterol in the phytosterol comprises brassicasterol, campesterol, stigmasterol and sitosterol;

[0136] The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

[0137] The lipid phytonutrient composition, wherein the long carbon chain fatty alkanol comprises C 22 -C 30 Straight chain fatty alkanol.

[0138] Example 4

[0139] A lipid phytonutrient composition, comprising, by weight percentage:

[0140] 10.0wt% natural vitamin E;

[0141] 10.0wt% phytosterols;

[0142] 30.0wt% squalene;

[0143] 5.0wt% long carbon chain fatty alkanol;

[0144] The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons;

[0145] Wherein, wt% is based on the total amount of the composition;

[0146] The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

[0147] The lipid phytonutrient composition has an iodine value of 150 g / 100 g, a saponification value of 2.4 mgKOH / g, an acid value of 0.2 mgKOH / g, and a peroxide value of 1.0 g / 100 g.

[0148] The lipid phytonutrient composition, wherein the ratio of tocopherol to tocotrienol in the natural vitamin E is:

[0149] Tocopherol: tocotrienol = 0.9:1.

[0150] The lipid phytonutrient composition, wherein the 4-desmethyl sterol in the phytosterol comprises brassicasterol, campesterol, stigmasterol and sitosterol;

[0151] The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

[0152] The lipid phytonutrient composition, wherein the long carbon chain fatty alkanol comprises C 22 -C 30 Straight chain fatty alkanol.

[0153] Example 5

[0154] A lipid phytonutrient composition, comprising, by weight percentage:

[0155] 30.0wt% natural vitamin E;

[0156] 20.0wt% phytosterols;

[0157] 20.0wt% squalene;

[0158] 3.0wt% of long carbon chain fatty alkanol;

[0159] The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons;

[0160] Wherein, wt% is based on the total amount of the composition;

[0161] The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

[0162] The lipid phytonutrient composition has an iodine value of 95 g / 100 g, a saponification value of 2.2 mgKOH / g, an acid value of 0.4 mgKOH / g, and a peroxide value of 0.9 g / 100 g.

[0163] The lipid phytonutrient composition, wherein the ratio of tocopherol to tocotrienol in the natural vitamin E is:

[0164] Tocopherol: tocotrienol = 1.1:1.

[0165] The lipid phytonutrient composition, wherein the 4-desmethyl sterol in the phytosterol comprises brassicasterol, campesterol, stigmasterol and sitosterol;

[0166] The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

[0167] The lipid phytonutrient composition, wherein the long carbon chain fatty alkanol comprises C 22 -C 30 Straight chain fatty alkanol.

[0168] Example 6

[0169] As Example 6, the composition of a typical rice bran oil deodorization distillate is as follows:

[0170]

[0171] A method for preparing a lipid phytonutrient composition comprises the following steps:

[0172] S1, converting fatty acids, glycerides and steroids contained in 1 kg of rice bran oil deodorization distillate to obtain lipid phytonutrients containing fatty acid alkyl esters, natural vitamin E, plant squalene, phytosterols and long-chain fatty alkanols.

[0173] Step S1 specifically includes:

[0174] S1.1, 1kg of rice bran oil deodorization distillate was mixed and dissolved with 400 ml of methanol, 20 ml of concentrated sulfuric acid was added dropwise for esterification at 70°C for 2h, and then deionized water was added to terminate the reaction, and the lower layer of alcohol, water and sulfuric acid was separated, and the oil phase was washed with hot water until neutral, and dehydrated for standby use;

[0175] S1.2, add 400 ml of methanol to the reaction solution obtained in step S1.1 again, add 30 ml of 30% sodium methoxide methanol solution dropwise, and carry out alcoholysis reaction at 70°C reflux condition for 1 hour, then add concentrated sulfuric acid to terminate the reaction, add deionized water, let it stand and separate, remove the mixed phase of methanol, water and sulfuric acid, and recover the methanol in steps S1.1 and S1.2 for reuse, wash the oil phase with hot water again until it is neutral, dehydrate for standby use, and obtain 995.0 g of oil phase, which contains converted lipid phytonutrients including fatty acid methyl esters, natural vitamin E, plant squalene, phytosterols, and long-chain fatty alkanols.

[0176] S2, the product obtained in step S1 is cooled and precipitated, crystallized and filtered to obtain crude sterols and mother liquor, wherein the crude sterols are 92.0 g, the phytosterol content is 75.5%, and the mother liquor is 900.0 g.

[0177] Step S2 specifically includes:

[0178] The oil phase after transformation in step S1 is cooled and precipitated. The process is divided into crystallization and crystal growth steps. The oil phase is cooled to 20°C at a rate of 1°C / h, and then crystals are grown at this temperature for 24h.

[0179] The contents of natural vitamin E, plant squalene, plant sterols, and long-chain fatty alkanols in the mother solution are as follows:

[0180]

[0181] S3, subjecting the mother liquor in step S2 to multi-stage distillation to separate and remove low-boiling point small molecule aldehydes, ketones, acids, fatty acid alkyl esters and plant hydrocarbon impurities, and enriching the concentrate of natural vitamin E, plant sterols, plant squalene and long-chain fatty alkanols.

[0182] The multi-stage distillation in step S3 is achieved by:

[0183] The first stage flash evaporation, adding 900.0g of the mother liquor in step S2 into the first stage flash evaporation tower, under the conditions of feed temperature of 100°C and vacuum degree of 200Pa of the tower top system, flash evaporation is performed to remove small molecular weight substances, including water, plant hydrocarbons, fatty acids, aldehydes and ketones;

[0184] The second stage distillation, the material after the first stage flash evaporation is continuously added to the second stage distillation tower, the tower bottom heating temperature is 160 ° C, the tower top temperature is maintained at 140 ° C, the vacuum condition is 50 Pa, the substances removed from the light components in the second stage distillation tower include fatty acid methyl ester, residual natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols;

[0185] Coupled with molecular distillation, the heavy components after the second stage distillation are continuously added into the molecular distillation system, the heating temperature is maintained at 240°C, the vacuum condition is 10Pa, and the light components after molecular distillation are 145g of lipid phytonutrient concentrate, wherein the lipid phytonutrient concentrate includes natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols, and residual plant asphalt.

[0186] The lipid nutrient concentrate after molecular distillation is composed of the following:

[0187]

[0188] S4, saponifying, extracting, decolorizing and refining the concentrate in step S3 in an alcohol-alkali solution to obtain the lipid phytonutrient composition.

[0189] Step S4 specifically includes:

[0190] S4.1, stirring and neutralizing the lipid phytonutrient concentrate enriched in step S3 with petroleum ether, methanol, deionized water and sodium hydroxide at 70° C. for 1 hour;

[0191] Among them, the addition ratio of petroleum ether to the enriched lipid phytonutrient concentrate is 2 mL / g, the addition ratio of methanol to the enriched lipid phytonutrient concentrate is 1 mL / g, and the addition ratio of deionized water to the enriched lipid phytonutrient concentrate is 0.5 mL / g.

[0192] S4.2, subjecting the solution obtained in step S4.1 to static extraction and stratification, and after the stratification interface is clear, stratification is performed, and the upper layer solution is retained for later use;

[0193] S4.3, washing the upper layer solution obtained in step S4.2 with water, allowing it to stand for stratification after washing, and stratifying again after the stratification interface is clear, leaving the upper layer solution for later use;

[0194] S4.4, adding activated carbon to the upper layer solution obtained in step S4.3, stirring to decolorize and purify, then filtering to remove the activated carbon, and using the filtrate for later use;

[0195] S4.5, recovering the organic solvent from the filtrate obtained in step S4.4, and molecularly distilling the filtrate to finally obtain 112 g of lipid plant nutrient composition;

[0196] S4.6, distilling the lower layer solution obtained in step S4.2, recovering the obtained low-carbon chain alcohol, and applying it to step S1, and the distillation condition is reduced pressure.

[0197] The lipid nutrition composition obtained in step S4.5 is as follows:

[0198]

[0199] The lipid phytonutrient composition has an iodine value of 80 g / 100 g, a saponification value of 1.5 mgKOH / g, an acid value of 1.0 mgKOH / g, and a peroxide value of 0.8 g / 100 g.

[0200] Example 7

[0201] The composition of the rice bran oil deodorized distillate is as follows:

[0202]

[0203] A method for preparing a lipid phytonutrient composition comprises the following steps:

[0204] S1, converting fatty acids, glycerides and steroids contained in 5.0 kg of rice bran oil deodorization distillate to obtain lipid phytonutrients containing fatty acid alkyl esters, natural vitamin E, plant squalene, phytosterols and long-chain fatty alkanols.

[0205] The material ratio and reaction conditions in step S1 were the same as those in Example 6, and 5.0 kg of oil phase was finally obtained.

[0206] S2, the product obtained in step S1 is cooled and precipitated, crystallized and separated by filter pressing to obtain crude sterols and mother liquor, wherein the crude sterols are 385.0 g, the phytosterol content is 55.5%, and the mother liquor is 4.6 kg.

[0207] Step S2 specifically includes:

[0208] The oil phase after transformation in step S1 is cooled and precipitated. The process is divided into crystallization and crystal growing steps. The oil phase is cooled to 15°C at a rate of 3°C / h, and then crystals are grown at this temperature for 12h.

[0209] The contents of natural vitamin E, plant squalene, plant sterols, and long-chain fatty alkanols in the mother solution are as follows:

[0210]

[0211] S3, subjecting the mother liquor in step S2 to multi-stage distillation to separate and remove low-boiling point small molecule aldehydes, ketones, acids, fatty acid alkyl esters and plant hydrocarbon impurities, and enriching the concentrate of natural vitamin E, plant sterols, plant squalene and long-chain fatty alkanols.

[0212] The multi-stage distillation in step S3 is achieved by:

[0213] The first stage flash evaporation, adding the mother liquor in step S2 to the first stage flash evaporation tower, under the conditions of feed temperature of 130°C and vacuum degree of 2000Pa of the tower top system, flash evaporation is performed to remove small molecular weight substances, including water, plant hydrocarbons, fatty acids, aldehydes and ketones;

[0214] The second stage distillation, the material after the first stage flash evaporation is continuously added to the second stage distillation tower, the tower bottom heating temperature is 200 ° C, the tower top temperature is maintained at 180 ° C, the vacuum condition is 200 Pa, the light components removed in the second stage distillation tower include fatty acid methyl ester, residual natural vitamin E, plant squalene, plant sterols and long carbon chain fatty alkanols;

[0215] Coupled with molecular distillation, the heavy components after the second stage distillation are continuously added into the molecular distillation system, the heating temperature is maintained at 220°C, the vacuum condition is 1Pa, and the light components after molecular distillation are 630.0g of lipid phytonutrient concentrate, wherein the lipid phytonutrient concentrate includes natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols, and residual plant asphalt.

[0216] The lipid nutrient concentrate after molecular distillation is composed of the following:

[0217]

[0218] S4, saponifying, extracting, decolorizing and refining the concentrate in step S3 in an alcohol-alkali solution to obtain the lipid phytonutrient composition.

[0219] Step S4 specifically includes:

[0220] S4.1, stirring and neutralizing the lipid phytonutrient concentrate enriched in step S3 with n-hexane, ethanol, deionized water and potassium hydroxide at 70° C. for 1 hour;

[0221] Among them, the addition ratio of n-hexane to the enriched lipid phytonutrient concentrate is 1 mL / g, the addition ratio of ethanol to the enriched lipid phytonutrient concentrate is 2 mL / g, and the addition ratio of deionized water to the enriched lipid phytonutrient concentrate is 0.25 mL / g.

[0222] S4.2, subjecting the solution obtained in step S4.1 to static extraction and stratification, and after the stratification interface is clear, stratification is performed, and the upper layer solution is retained for later use;

[0223] S4.3, washing the upper layer solution obtained in step S4.2 with water, allowing it to stand for stratification after washing, and stratifying again after the stratification interface is clear, leaving the upper layer solution for later use;

[0224] S4.4, adding activated carbon to the upper layer solution obtained in step S4.3, stirring to decolorize and purify, then filtering to remove the activated carbon, and using the filtrate for later use;

[0225] S4.5, recovering the organic solvent from the filtrate obtained in step S4.4, and subjecting the filtrate to molecular distillation to obtain 510.0 g of a lipid phytonutrient composition;

[0226] S4.6, distilling the lower layer solution obtained in step S4.2, recovering the obtained low-carbon chain alcohol, and applying it to step S1, and the distillation condition is normal pressure.

[0227] The lipid nutrition composition obtained in step S4.5 is composed as follows:

[0228]

[0229] The lipid phytonutrient composition has an iodine value of 120 g / 100 g, a saponification value of 2.0 mgKOH / g, an acid value of 1.5 mgKOH / g, and a peroxide value of 1.0 g / 100 g.

[0230] Example 8

[0231] The composition of the rice bran oil deodorized distillate is as follows:

[0232]

[0233] A method for preparing a lipid phytonutrient composition comprises the following steps:

[0234] S1, converting fatty acids, glycerides and steroids contained in 1000 kg of rice bran oil deodorization distillate to obtain lipid phytonutrients containing fatty acid alkyl esters, natural vitamin E, plant squalene, phytosterols and long-chain fatty alkanols.

[0235] The material ratio and reaction conditions in step S1 were the same as those in Example 6, and 990 kg of oil phase was finally obtained.

[0236] S2, the product obtained in step S1 is cooled and precipitated, crystallized and filtered to obtain crude sterols and mother liquor, wherein the crude sterols are 110 kg, the phytosterol content is 70.5%, and the mother liquor is 870 kg.

[0237] Step S2 specifically includes:

[0238] The oil phase after transformation in step S1 is cooled and precipitated. The process is divided into crystallization and crystal growing steps. The oil phase is cooled to 18°C ​​at a rate of 2°C / h, and then the crystal is grown at this temperature for 18h.

[0239] The contents of natural vitamin E, plant squalene, plant sterols, and long-chain fatty alkanols in the mother solution are as follows:

[0240]

[0241] S3, subjecting the mother liquor in step S2 to multi-stage distillation to separate and remove low-boiling point small molecule aldehydes, ketones, acids, fatty acid alkyl esters and plant hydrocarbon impurities, and enriching the concentrate of natural vitamin E, plant sterols, plant squalene and long-chain fatty alkanols.

[0242] The multi-stage distillation in step S3 is achieved by:

[0243] The first stage flash evaporation, adding the mother liquor in step S2 to the first stage flash evaporation tower, under the conditions of feed temperature of 120°C and vacuum degree of 1000Pa of the tower top system, flash evaporation is performed to remove small molecular weight substances, including water, plant hydrocarbons, fatty acids, aldehydes and ketones;

[0244] The second stage distillation, the material after the first stage flash evaporation is continuously added to the second stage distillation tower, the tower bottom heating temperature is 180 ° C, the tower top temperature is maintained at 160 ° C, the vacuum condition is 100 Pa, the light components removed in the second stage distillation tower include fatty acid methyl ester, residual natural vitamin E, plant squalene, plant sterols and long carbon chain fatty alkanols;

[0245] Coupled with molecular distillation, the heavy components after the second stage distillation are continuously added into the molecular distillation system, the heating temperature is maintained at 230°C, the vacuum condition is 5Pa, and the light components after molecular distillation are 125kg of lipid phytonutrient concentrate, wherein the lipid phytonutrient concentrate includes natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols, and residual plant asphalt.

[0246] The lipid nutrient concentrate after molecular distillation is composed of the following:

[0247]

[0248] S4, saponifying, extracting, decolorizing and refining the concentrate in step S3 in an alcohol-alkali solution to obtain the lipid phytonutrient composition.

[0249] Step S4 specifically includes:

[0250] S4.1, stirring and neutralizing the lipid phytonutrient concentrate enriched in step S3 with n-heptane, isopropanol, deionized water and sodium hydroxide at 70° C. for 1 hour;

[0251] Among them, the addition ratio of n-heptane to the enriched lipid phytonutrient concentrate is 1.5 mL / g, the addition ratio of isopropanol to the enriched lipid phytonutrient concentrate is 1 mL / g, and the addition ratio of deionized water to the enriched lipid phytonutrient concentrate is 0.5 mL / g.

[0252] S4.2, subjecting the solution obtained in step S4.1 to static extraction and stratification, and after the stratification interface is clear, stratification is performed, and the upper layer solution is retained for later use;

[0253] S4.3, washing the upper layer solution obtained in step S4.2 with water, allowing it to stand for stratification after washing, and stratifying again after the stratification interface is clear, leaving the upper layer solution for later use;

[0254] S4.4, adding activated carbon to the upper layer solution obtained in step S4.3, stirring to decolorize and purify, then filtering to remove the activated carbon, and using the filtrate for later use;

[0255] S4.5, recovering the organic solvent from the filtrate obtained in step S4.4, and then molecularly distilling the filtrate to obtain 100 kg of lipid plant nutrient composition;

[0256] S4.6, distilling the lower layer solution obtained in step S4.2, recovering the obtained low-carbon chain alcohol, and applying it to step S1, and the distillation condition is reduced pressure.

[0257] The lipid nutrition composition obtained in step S4.5 is composed as follows:

[0258]

[0259] The lipid phytonutrient composition has an iodine value of 145 g / 100 g, a saponification value of 0.5 mgKOH / g, an acid value of 0.5 mgKOH / g, and a peroxide value of 0.5 g / 100 g.

[0260] Example 9

[0261] The lipid phytonutrient composition in Example 1 is used to prepare a skin care preparation for human skin, and the skin care preparation at least includes the lipid phytonutrient composition.

[0262] Example 10

[0263] A lip balm is prepared using the lipid phytonutrient composition in Example 2, and the lip balm at least includes the lipid phytonutrient composition.

[0264] Embodiment 11

[0265] The lipid phytonutrient composition in Example 3 is used to prepare a feed additive, which at least includes the lipid phytonutrient composition.

[0266] Example 12

[0267] A dietary supplement is prepared using the lipid phytonutrient composition of Example 4, wherein the dietary supplement comprises at least the lipid phytonutrient composition.

[0268] In summary, according to the lipid phytonutrient composition provided by the present invention, its preparation method and application, the lipid phytonutrient composition comprises natural vitamin E, plant squalene, phytosterols, long carbon chain fatty alkanols, plant waxes or plant hydrocarbons and other ingredients. The present invention performs esterification / ester exchange, multi-stage distillation and saponification extraction, refining and other steps on the rice bran oil deodorization distillate to finally obtain the lipid phytonutrient composition, and the lipid phytonutrient composition can be applied to multiple fields such as food, feed and cosmetics. Therefore, the present invention extracts a new and useful composition from the rice bran oil deodorization distillate, further expanding the application value of the rice bran oil deodorization distillate.

[0269] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for preparing a lipid phytonutrient composition, characterized in that: The following steps are involved: S1, converting fatty acids, glycerides and steroids contained in a deodorized distillate of rice bran oil to obtain lipid phytonutrients containing fatty acid alkyl esters, natural vitamin E, plant squalene, phytosterols and long-chain fatty alkanols; S2, cooling the product obtained in step S1, crystallizing and filtering to obtain crude sterol and mother liquor; S3, subjecting the mother liquor in step S2 to multi-stage distillation to separate and remove low-boiling-point small-molecule aldehydes, ketones, acids, fatty acid alkyl esters and plant hydrocarbon impurities, and enriching the concentrate of natural vitamin E, phytosterols, plant squalene and long-chain fatty alkanols; S4, saponifying, extracting, decolorizing and refining the concentrate in step S3 in an alcohol-alkali solution to obtain the lipid plant nutrient composition; Step S1 specifically includes: S1.1, dissolving the rice bran oil deodorization distillate with methanol, dropping concentrated sulfuric acid as a catalyst to carry out methyl esterification reaction, then adding deionized water to terminate the reaction, allowing the layers to stand, separating the lower layer of alcohol, water and sulfuric acid mixed phase, washing the oil phase with hot water until neutral, and dehydrating for standby use; S1.2, add a methanol solution of an alkaline catalyst to the reaction solution obtained in step S1.1 to carry out alcoholysis reaction, then add concentrated sulfuric acid to terminate the reaction, then add deionized water, let it stand for stratification, remove the mixed phase of methanol, water and sulfuric acid, and recover the methanol in step S1.1 and step S1.2 for reuse, wash the oil phase with hot water again until it is neutral, dehydrate and set aside, the oil phase contains the converted lipid plant nutrients including fatty acid methyl esters, natural vitamin E, plant squalene, plant sterols, and long-chain fatty alkanols; Step S2 specifically includes: The oil phase after the transformation in step S1 is cooled and precipitated. The process is divided into crystallization and crystal growth steps. The oil phase is cooled to 15-20°C at a rate of 1-3°C / h, and then crystals are grown at this temperature for 12-24h; The multi-stage distillation in step S3 is achieved by: The first stage flash evaporation is to add the mother liquor in step S2 to the first stage flash evaporation tower, and the feed temperature is 100-130°C, and the vacuum degree of the tower top system is 200-2000Pa, and the low molecular weight substances are flashed to remove the low molecular weight substances, including water, plant hydrocarbons, fatty acids, aldehydes and ketones; The second stage distillation is to continuously add the materials after the first stage flash distillation into the second stage distillation tower, the tower bottom is heated to 160-200°C, the tower top temperature is maintained at 140-180°C, and the vacuum condition is 50-200Pa. The substances removed from the light components in the second stage distillation tower include fatty acid methyl esters, residual natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols; Coupled with molecular distillation, the heavy components after the second stage distillation are continuously added into the molecular distillation system, the heating temperature is maintained at 220-240°C, the vacuum condition is 1-10Pa, and the light components after molecular distillation are lipid phytonutrient concentrates, wherein the lipid phytonutrient concentrates include natural vitamin E, plant squalene, plant sterols and long-chain fatty alkanols, and residual plant asphalt; Step S4 specifically includes: S4.1, the lipid phytonutrient concentrate enriched in step S3 is reacted with an organic solvent, a low-carbon chain alcohol, deionized water, and a caustic soda at 70-80° C. under stirring for 1-2 hours; S4.2, subjecting the solution obtained in step S4.1 to static extraction and stratification, and after the stratification interface is clear, stratify the layers and keep the upper layer solution for later use; S4.3, washing the upper layer solution obtained in step S4.2 with water, standing it for stratification after washing, and stratifying it again after the stratification interface is clear, and keeping the upper layer solution for later use; S4.4, adding activated carbon to the upper solution obtained in step S4.3, stirring to decolorize and purify, then filtering to remove the activated carbon, and using the filtrate for later use; S4.5, recovering the organic solvent from the filtrate obtained in step S4.4, and then molecularly distilling the filtrate to obtain the lipid plant nutrient composition; S4.6, distilling the lower layer solution obtained in step S4.2, recovering the obtained low-carbon chain alcohol, and applying it to step S1, the distillation condition is normal pressure or reduced pressure; Wherein, the lipid phytonutrient composition comprises, by weight percentage: 10-30.0wt% natural vitamin E; 5-20.0wt% of phytosterols; 10-30.0wt% squalene; 1-10.0wt% of long carbon chain fatty alkanol; The rest is unsaponifiable vegetable waxes or vegetable hydrocarbons; Wherein, wt% is based on the total amount of the composition; The lipid phytonutrient composition is derived from deodorized distillate of rice bran oil, the natural vitamin E comprises tocopherol and tocotrienol, and the phytosterol comprises 4-desmethyl sterol and 4,4-dimethyl sterol.

2. The method for preparing the lipid phytonutrient composition according to claim 1, characterized in that: The lipid phytonutrient composition has an iodine value of 50-150 g / 100 g, a saponification value of less than 3 mg KOH / g, an acid value of less than 1 mg KOH / g, and a peroxide value of less than 5 g / 100 g.

3. The method for preparing the lipid phytonutrient composition according to claim 1, characterized in that: The content ratio of tocopherol to tocotrienol in the natural vitamin E is: tocopherol: tocotrienol = 0.8-1.2:

1.

4. The method for preparing the lipid phytonutrient composition according to claim 1, characterized in that: The 4-demethyl sterols in the phytosterols include brassicasterol, campesterol, stigmasterol and sitosterol; The 4,4-dimethyl sterols in the phytosterols include cycloartanol and 24-methylenecycloartanol.

5. The method for preparing the lipid phytonutrient composition according to claim 1, characterized in that: The long carbon chain fatty alkanol comprises C22-C30 straight chain fatty alkanol.

6. The method for preparing the lipid phytonutrient composition according to claim 1, characterized in that: In the step S4.1, the organic solvent is one of petroleum ether, n-hexane and n-heptane with a boiling range of 90-120° C., and the addition ratio of the organic solvent to the enriched lipid phytonutrient concentrate is 1-2 mL / g.

7. The method for preparing the lipid phytonutrient composition according to claim 1, characterized in that: In the step S4.1, the low-chain alcohol is one of methanol, ethanol, and isopropanol, the addition ratio of the low-chain alcohol to the enriched lipid phytonutrient concentrate is 0.5-2mL / g, and the addition ratio of the deionized water to the enriched lipid phytonutrient concentrate is 0.25-0.5mL / g.

8. The method for preparing the lipid phytonutrient composition according to claim 1, characterized in that: In the step S4.1, the caustic soda is sodium hydroxide or potassium hydroxide.

9. Use of the lipid phytonutrient composition according to any one of claims 1 to 5 in the preparation of a skin care preparation for human skin.

10. Use of the lipid phytonutrient composition according to any one of claims 1 to 5 in the preparation of lip balm.

11. Use of the lipid phytonutrient composition according to any one of claims 1 to 5 in the preparation of feed additives.

12. Use of the lipid phytonutrient composition according to any one of claims 1 to 5 in the preparation of a dietary supplement.

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