Magnetic field controlled three-dimensional oriented biomaterial 3D printer and printing method

Abstract

A magnetic field controlled three-dimensional orientation biological material 3D printer and a fiber three-dimensional orientation printing method using the same, wherein the magnetic field controlled three-dimensional orientation biological material 3D printer is provided with a main body, a fixed platform device for adhering a printing material layer to which a fiber responding to a magnetic field change is added, a magnetic field adjusting device for generating a 360-degree adjustable magnetic field to the printing material layer, a lifting device for driving the fixed platform device to lift and fall, and a light control device for curing and forming the printing material layer. The magnetic field adjusting device of the magnetic field controlled three-dimensional orientation biological material 3D printer can rotate 360 degrees around the periphery of the fixed platform device, and at least one pair of magnets are respectively located on the opposite sides of the periphery of the bottom surface of the curing area, so that a magnetic field can be generated on the opposite sides of the bottom surface of the curing area and rotate 360 degrees, so that the orientation direction of the fiber can be flexibly adjusted, and the generated magnetic field can be uniformly distributed and completely cover the curing area.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological printing, in particular to a magnetic field controlled three-dimensional orientation biological material 3D printer and a fiber three-dimensional orientation printing method using the same. BACKGROUND

[0002] Biological 3D printing is to use the basic principles and methods of 3D printing discrete / piling forming to print biological materials (including natural biological materials and artificially synthesized biological materials) or cell suspensions in a controlled manner to form required implants, cell three-dimensional structures or artificial tissue organs with biological activity. Biological 3D printing, as a new technology at the intersection of life science and modern manufacturing science, is conducive to building various three-dimensional biomimetic structures required in tissue engineering.

[0003] Studies have shown that the orientation of cells is closely related to the mechanical stimulation and structure morphology of the external environment. The main method for controlling the orientation of cells is to make a directional groove or stripe structure on the surface of a cell growth substrate in advance, and then inoculate cells on the substrate, so that the cells will grow in a directional arrangement along the groove or stripe structure to achieve directional arrangement control of the cells. However, this method can only achieve two-dimensional directional arrangement of cells on the surface of a substrate with a specific morphology, and cannot be used for biological 3D printing to build any required cell-carrying structure.

[0004] Currently, a method for 3D printing of a fiber product with multiple orientations is provided by adding fibers that can respond to changes in a magnetic field in printing ink, controlling the orientation of the fibers, and then inoculating cells on the product, so that the oriented fibers will induce the growth direction of the cells, and finally achieve three-dimensional orientation control of the cells.

[0005] A Chinese invention patent with publication number CN106738898A discloses a programmable directional short fiber reinforced composite material 3D printing method and device. The device is composed of a main frame, a feeding bin, a material laying device, a forming bin, a waste bin, a digital mask photopolymerization system, and a controllable magnetic field system. The device forms the laying of the material with a slice layer thickness by moving the feeding bin upward, moving the forming bin downward, and moving the material laying device left and right, and then the controllable magnetic field system generates a magnetic field on the printing material by moving a magnetic field source on two opposite sides of the forming bin and rotating 180° in the horizontal plane. However, the device can only generate a magnetic field on one side of the printing material, resulting in uneven distribution of the magnetic field in the solidification area and difficulty in completely surrounding the solidification area of the forming bin, thereby affecting the orientation effect of the fibers.

[0006] Therefore, in view of the deficiencies of the prior art, it is necessary to provide a magnetic field controlled three-dimensional orientation biological material 3D printer and a fiber three-dimensional orientation printing method using the same to solve the deficiencies of the prior art. SUMMARY

[0007] One of the purposes of the present application is to provide a magnetic field controlled three-dimensional oriented biomaterial 3D printer to avoid the shortcomings of the prior art. The magnetic field generated by the magnetic field controlled three-dimensional oriented biomaterial 3D printer can be uniformly distributed and completely cover the solidification area.

[0008] The above-mentioned purposes of the present application are achieved by the following technical measures:

[0009] The present application provides a magnetic field controlled three-dimensional oriented biomaterial 3D printer, which comprises a main body and a light control device, and is provided with a fixed platform device for adhering a printing material layer added with a fiber responding to magnetic field changes, a magnetic field adjusting device for generating a magnetic field on the printing material layer, and a lifting device for driving the fixed platform device to lift, the lifting device is fixedly assembled to the main body, the fixed platform device is drivingly assembled with the lifting device, and the magnetic field adjusting device is 360° rotatably assembled to the outer periphery of the fixed platform device.

[0010] There is a solidification area for solidifying and forming the printing material layer between the fixed platform device and the light control device, and at least one pair of magnets of the magnetic field adjusting device is respectively located at the outer periphery of the bottom surface of the solidification area and can rotate 360°.

[0011] Preferably, the length of the magnet is not less than the maximum length of the lower bottom surface of the fixed platform device.

[0012] Preferably, the magnets are symmetrically arranged on the bottom surface of the solidification area.

[0013] Preferably, there is a solidification area for solidifying and forming the printing material layer between the fixed platform device and the light control device, and the magnetic field generated by the magnetic field adjusting device is located at the outer periphery of the bottom surface of the solidification area.

[0014] Preferably, the fixed platform device is provided with a cantilever, a support and an adhering column, one end of the cantilever is drivingly assembled with the lifting device, one end of the cantilever is fixedly assembled with the support, the adhering column is fixedly assembled below the support, the magnetic field adjusting device is assembled to the cantilever, the magnetic field adjusting device is located at the outer periphery of the adhering column, and the adhering column is located directly above the light control device.

[0015] Preferably, the magnetic field adjusting device is provided with a driving assembly, a rotating assembly, a magnet, and a piston rod for driving the magnet to rotate and keeping the magnet on the bottom surface of the solidification area, the driving assembly is fixedly arranged on the cantilever, the rotating assembly is in transmission connection with the driving assembly, the rotating assembly is rotatably arranged on the outer surface of the support, one end of the piston rod is fixedly connected with the rotating assembly, and the other end of the piston rod is fixedly connected with the magnet.

[0016] Preferably, the piston rod is provided with a first sleeve, a plug and an inner rod, one end of the first sleeve is fixedly connected with the rotating assembly, one end of the inner rod is movably sleeved on the other end of the first sleeve, the other end of the first sleeve is in sliding abutment with the outer surface of the inner rod, the magnet is fixedly arranged on the other end of the inner rod, the plug is fixedly connected with one end of the inner rod, and the outer surface of the plug is in sliding abutment with the inner surface of the first sleeve.

[0017] Preferably, the rotating assembly is provided with a gear ring, a rotating disc and a second sleeve, the gear ring is fixedly arranged on the upper outer surface of the second sleeve, the rotating disc is fixedly arranged on the lower part of the second sleeve, and the second sleeve is rotatably sleeved on the outer surface of the support.

[0018] Preferably, the support is provided with a fixed rod, a pair of fixing members and a bolt, the pair of fixing members are respectively fixedly connected with the opposite sides of the cantilever, the bolt penetrates through the upper part of the fixed rod and is arranged on the fixing members at both ends, and the end of the bolt penetrates through the magnetic field adjusting device and is fixedly arranged on the adhesive column.

[0019] Preferably, the driving assembly is provided with a first motor and a gear, the first motor is fixedly arranged on the cantilever, the gear is fixedly connected with the rotating shaft of the first motor, and the gear is in meshing connection with the gear ring.

[0020] Preferably, the lifting device is provided with a second motor, a support rod, a sliding rail, a sliding block, a limiting block and a lead screw, one end of the support rod and the second motor are respectively fixedly arranged on the main body, one end of the lead screw is fixedly connected with the rotating shaft of the second motor, the other end of the support rod and the other end of the lead screw are respectively fixedly connected with the limiting block, the support rod and the lead screw are arranged in parallel, the sliding rail is fixedly arranged on one side surface of the support rod, the sliding rail is located between the support rod and the lead screw, one side of the sliding block is in sliding connection with the sliding rail, the other side of the sliding block is fixedly arranged on the cantilever, the middle of the sliding block is provided with an internal threaded hole, and the lead screw is sleeved on the internal threaded hole.

[0021] The magnetic field controlled three-dimensional orientation biological material 3D printer of the present application is also provided with a control panel fixedly assembled to the main body.

[0022] Preferably, the above-mentioned magnets are at least provided in pairs, the above-mentioned piston rods are at least provided in pairs, and the number of the piston rods is twice the number of the pistons.

[0023] Preferably, the above-mentioned magnets are located at the opposite two sides of the bottom surface of the solidification area.

[0024] Preferably, the above-mentioned light control device is an LCD screen.

[0025] Preferably, the length of the above-mentioned magnets is not less than the maximum length of the lower bottom surface of the adhesive column.

[0026] Another object of the present application is to provide a fiber three-dimensional orientation printing method to avoid the shortcomings of the prior art. The fiber three-dimensional orientation printing method can print a 3D structure product with good fiber orientation effect.

[0027] The above-mentioned objects of the present application are achieved by the following technical measures:

[0028] The present application provides a fiber three-dimensional orientation printing method, which is performed by using the above-mentioned magnetic field controlled three-dimensional orientation biological material 3D printer.

[0029] The magnetic field controlled three-dimensional orientation biological material 3D printer of the present application and the fiber three-dimensional orientation printing method using the same, wherein the magnetic field controlled three-dimensional orientation biological material 3D printer is provided with a main body, a fixed platform device for adhering a printing material layer added with fibers responding to magnetic field changes, a magnetic field adjusting device for generating a magnetic field on the printing material layer, a lifting device for driving the fixed platform device to lift, and a light control device for curing and forming the printing material layer, the lifting device and the light control device are fixedly assembled to the main body, the fixed platform device is transmissionally assembled with the lifting device, the magnetic field adjusting device is 360° rotationally assembled to the outer periphery of the fixed platform device, and the light control device is fixedly assembled to the main body; there is a solidification area for curing and forming the printing material layer between the fixed platform device and the light control device, and at least one pair of magnets of the magnetic field adjusting device are respectively located at the opposite two sides of the outer periphery of the bottom surface of the solidification area and can rotate 360°. The magnetic field adjusting device of the magnetic field controlled three-dimensional orientation biological material 3D printer of the present application can rotate 360° at the outer periphery of the fixed platform device, and at least one pair of magnets are respectively located at the opposite two sides of the outer periphery of the bottom surface of the solidification area, so that a magnetic field can be generated at the opposite two sides of the bottom surface of the solidification area and rotate 360°, so that the orientation direction of the fibers can be flexibly adjusted, and the generated magnetic field can be uniformly distributed and completely cover the solidification area. BRIEF DESCRIPTION OF DRAWINGS

[0030] The application will be further described with reference to the drawings, which do not limit the application in any way.

[0031] Figure 1 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0032] Figure 2 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field. Figure 1 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0033] Figure 3 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field. Figure 1 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0034] Figure 4 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0035] Figure 5 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field. Figure 4 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0036] Figure 6 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field. Figure 5 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0037] Figure 7 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0038] Figure 8 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0039] Figure 9 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0040] Figure 10 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0041] Figure 11 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0042] Figure 12 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0043] Figure 13 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0044] Figure 14 Structure diagram of the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field.

[0045] In the 3D printer for controlling three-dimensional orientation of biological materials by magnetic field, the fixed platform device 100, Figures 1 to 14 includes:

[0046] the fixed platform device 100,

[0047] cantilever 110,

[0048] support 120, fixed rod 121, fixing piece 122, bolt 123,

[0049] adhesion column 130,

[0050] magnetic field adjusting device 200,

[0051] drive assembly 210, first motor 211, gear 212,

[0052] rotary assembly 220, gear ring 221, rotary disc 222, second sleeve 223,

[0053] piston rod 230, first sleeve 231, plug 232, inner rod 233,

[0054] magnet 240,

[0055] lifting device 300, support rod 310, sliding rail 320, sliding block 330, limiting block 340, lead screw 350,

[0056] light control device 400, main body 500, solidification area 600, control panel 700. DETAILED DESCRIPTION

[0057] The technical solutions of the present application are further described in combination with the following examples.

[0058] The magnetic field control three-dimensional oriented biomaterial 3D printer of the present application is a 3D printer based on digital light processing forming technology (DLP) for printing.

[0059] The principle of digital light processing forming technology is to slice a 3D model by layering software, fill a liquid tank with liquid printing ink, and then project light out after digital processing of each layer of slice image data by a digital micro-mirror (DMD), so as to realize control of exposure energy and exposure pattern by a digital micro-mirror chip. One thin layer is solidified each time, the workbench is moved by a layer thickness after solidification of one layer, and the next layer is produced. The newly solidified layer is firmly bonded to the previous layer, and the complete product is formed by repeating the above steps.

[0060] Example 1

[0061] A magnetic field control three-dimensional oriented biomaterial 3D printer, as shown in Figures 1 to 13 Fig. 1, is provided with a main body 500, a light control device 400, a fixed platform device 100 for adhering to a printing material layer to which a fiber responsive to magnetic field changes is added, a magnetic field adjusting device 200 for generating a magnetic field to the printing material layer, and a lifting device 300 for driving the fixed platform device 100 to lift.

[0062] Need to say, the magnetic field control three-dimensional orientation of the biological material 3D printer in normal use, the ground direction defined below.

[0063] Wherein, the lifting device 300 fixedly assembled in the main body 500, fixed platform device 100 and lifting device 300 transmission assembly, magnetic field adjusting device 200 can be 360° rotation assembly in the outer periphery of the fixed platform device 100. Light control device 400 fixedly assembled in the main body 500, fixed platform device 100 is located in the light control device 400 directly above, the light control device 400 of the embodiment is specifically for LCD screen.

[0064] Need to explain, magnetic field adjusting device 200 directly installed in the outer periphery of the fixed platform device 100 so as to reduce the occupation space of the printer, and the magnetic field adjusting device 200 can be fixed platform device 100 360° rotation, improve the convenience of magnetic field direction control.

[0065] Between the fixed platform device 100 and the light control device 400, there is a curing area 600 for curing the printing material layer, and at least one pair of magnets 240 of the magnetic field adjusting device 200 is located at the outer periphery of the bottom surface of the curing area 600 and can rotate 360°.

[0066] Need to explain, each time in the bottom of the curing area 600 (i.e. the surface of the light control device 400) inject a certain amount of printing material added with response to the magnetic field change fiber, and then the fixed platform device 100 in the lower while the magnetic field adjusting device 200 adjusts the angle of the magnetic field, when the fixed platform device 100 is lowered to the printing material and extruding it, the printing material is spread to get the curing material layer, the angle of the magnetic field adjusting device 200 is adjusted, the fiber orientation in the curing material layer, finally the light control device 400 according to the required 3D printing product shape pattern, display pattern light in the corresponding part and project on the curing material layer, so as to form the local curing material layer in the specific position.

[0067] In actual operation, the light control device 400 is made of material that does not adhere to the printing material, and the bottom surface of the fixed platform device 100 is made of material that has good adhesion to the printing material, so the printing material adheres to the bottom surface of the fixed platform device 100.

[0068] Need to explain, the printing material injected into the bottom of the curing area 600 can be injected by artificial injection, or can be injected by external injection device.

[0069] The length of the magnet 240 is not less than the maximum length of the lower bottom surface of the fixed platform device 100, and specifically the length of the magnet 240 is not less than the maximum length of the lower bottom surface of the adhesion column 130. The magnet 240 is symmetrically arranged on the bottom surface of the solidification area 600, and specifically the magnet 240 is arranged opposite to two side surfaces of the bottom surface of the solidification area 600.

[0070] It should be noted that the length of the magnet 240 and the maximum length of the lower bottom surface of the fixed platform device 100 are arranged to enable the magnetic field generated by the magnet 240 to completely cover the bottom surface of the solidification area 600, and the symmetric arrangement of the magnet 240 enables the generation of a uniform magnetic field, and also improves the strength of the magnetic field lines.

[0071] The fixed platform device 100 is provided with a cantilever 110, a support 120, and an adhesion column 130. One end of the cantilever 110 is drivingly assembled with the lifting device 300, one end of the cantilever 110 is fixedly assembled with the support 120, the adhesion column 130 is fixedly assembled below the support 120, the magnetic field adjusting device 200 is assembled on the cantilever 110, and the magnetic field adjusting device 200 is located outside the adhesion column 130, and the adhesion column 130 is located directly above the light control device 400.

[0072] The support 120 is provided with a fixed rod 121, a pair of fixing members 122, and a bolt 123. The pair of fixing members 122 are respectively fixedly connected to the opposite two sides of the cantilever 110. The bolt 123 penetrates the upper portion of the fixed rod 121 and is assembled at both ends of the fixed rod 121 to the fixing members 122. The end of the bolt 123 penetrates the magnetic field adjusting device 200 and is fixedly assembled to the adhesion column 130.

[0073] The magnetic field adjusting device 200 is provided with a driving assembly 210, a rotating assembly 220, a piston rod 230 for driving the magnet 240 to rotate and enabling the magnet 240 to always be located on the bottom surface of the solidification area 600. The driving assembly 210 is fixedly assembled on the cantilever 110. The rotating assembly 220 is drivingly connected to the driving assembly 210, and the rotating assembly 220 is rotatably sleeved on the outer surface of the support 120. One end of the piston rod 230 is fixedly connected to the rotating assembly 220, and the other end of the piston rod 230 is fixedly connected to the magnet 240. The rotating assembly 220 is provided with a gear ring 221, a rotating disc 222, and a second sleeve 223. The gear ring 221 is fixedly assembled on the upper outer surface of the second sleeve 223. The rotating disc 222 is fixedly assembled on the lower portion of the second sleeve 223, and the second sleeve 223 is rotatably sleeved on the outer surface of the support 120.

[0074] The driving assembly 210 is provided with a first motor 211 and a gear 212. The first motor 211 is fixedly assembled on the cantilever 110. The gear 212 is fixedly connected to the rotating shaft of the first motor 211, and the gear 212 is engaged with the gear ring 221.

[0075] The rotating assembly 220 is sleeved on the outer surface of the support 120, and under the action of the driving assembly 210, the rotating assembly 220 can rotate around the central axis where the support 120 is located, so as to adjust the angle of the magnetic field lines.

[0076] The piston rod 230 is provided with a first sleeve 231, a plug 232 and an inner rod 233, one end of the first sleeve 231 is fixedly connected with the rotating assembly 220, one end of the inner rod 233 is movably sleeved on the other end of the first sleeve 231, and the other end of the first sleeve 231 is in sliding abutment with the outer surface of the inner rod 233, the magnet 240 is fixedly assembled on the other end of the inner rod 233, the plug 232 is fixedly connected with one end of the inner rod 233, and the outer surface of the plug 232 is in sliding abutment with the inner surface of the first sleeve 231.

[0077] It should be noted that, because the inner rod 233 and the first sleeve 231 are movably sleeved, the magnet 240 can rotate with the rotation of the rotating assembly 220, and under the action of the gravity of the magnet 240 itself, the magnet 240 is always in abutment with the bottom surface of the solidification area 600 (i.e. the surface of the light control device 400), so that the generated magnetic field lines can always pass through the layer of material to be solidified.

[0078] The lifting device 300 is provided with a second motor (not shown in the figure), a support rod 310, a sliding rail 320, a sliding block 330, a limiting block 340 and a lead screw 350, one end of the support rod 310 and the second motor are fixedly assembled on the main body 500, one end of the lead screw 350 is fixedly connected with the rotating shaft of the second motor, the other end of the support rod 310 and the other end of the lead screw 350 are fixedly connected with the limiting block 340, and the support rod 310 and the lead screw 350 are arranged in parallel, the sliding rail 320 is fixedly assembled on one side surface of the support rod 310, the sliding rail 320 is located between the support rod 310 and the lead screw 350, one side of the sliding block 330 is in sliding connection with the sliding rail 320, the other side of the sliding block 330 is fixedly assembled with the cantilever 110, the middle of the sliding block 330 is provided with an internally threaded hole, and the lead screw 350 is sleeved on the internally threaded hole.

[0079] It should be noted that, the lifting device 300 converts the rotary motion of the lead screw 350 into the up-down linear motion of the sliding block 330 through the lead screw 350 and the sliding block 330 with the internally threaded hole, so as to drive the fixed platform device 100 to move up and down as a whole, gradually increase the height of the solidification area 600, and accommodate the 3D printing product stacked layer by layer.

[0080] The control panel 700 is fixedly assembled on the main body 500.

[0081] It should be noted that the control panel 700 is a control operation device for controlling the lowering of the lifting device 300, the rotation of the magnetic field adjusting device 200, and the display shape of the pattern light of the light control device 400, and the specific implementation of the lifting device 300, the magnetic field adjusting device 200, and the light control device 400 by the control panel 700 is not the focus of the application, and those skilled in the art can set it according to the actual situation, which will not be repeated here.

[0082] The magnet 240 of the application is provided with at least one pair, and the piston rod 230 is provided with at least two pairs, and the number of the piston rod 230 is twice the number of the piston. The magnet 240 of the embodiment is provided with one pair, and the piston is provided with two pairs.

[0083] The use method of the magnetic field control three-dimensional oriented biomaterial 3D printer of the application is as follows: the printing material added with fibers responding to magnetic field changes is injected at the bottom of the solidification area 600 (i.e. the surface of the light control device 400), then the downward movement of the fixed platform device 100 is fixed, and the magnetic field adjusting device 200 adjusts the angle of the magnetic field, when the fixed platform device 100 is lowered to the printing material and extruded, the printing material is spread to obtain a layer of to-be-solidified material, at this time, the angle adjustment of the magnetic field adjusting device 200 is completed, and the magnetic field induction is generated on the layer of to-be-solidified material at the bottom of the solidification area 600, so that the fibers of the layer of to-be-solidified material are oriented, finally, the light control device 400 displays the pattern light according to the pattern of the required 3D printed product shape, and projects the pattern light on the layer of to-be-solidified material, so as to form the local layer of to-be-solidified material at the specific position. When the current layer of to-be-solidified material is formed, the fixed platform device 100 is completely raised, and a certain printing material added with fibers responding to magnetic field changes is injected at the bottom of the solidification area 600, then the fixed platform device 100 is lowered while the angle of the magnetic field adjusting device 200 is adjusted, the fixed platform device 100 adheres the printing material to the lower layer of the formed printing material layer, the angle of the magnetic field adjusting device 200 is adjusted, and the bottom layer of the to-be-printed material layer is oriented, finally, the light control device 400 performs specific pattern solidification on the bottom layer of the to-be-printed material layer, and finally, the multi-layer printing material layer is stacked to form a 3D structure fiber product with multiple orientations.

[0084] The magnetic field control three-dimensional orientation biological material 3D printer magnetic field adjusting device 200 can rotate 360° around the outer periphery of the fixed platform device 100, and at least one pair of magnets 240 are respectively located on the opposite sides of the outer periphery of the bottom surface of the solidification area 600, so that a magnetic field can be generated on the opposite sides of the bottom surface of the solidification area 600 and rotate 360°, so that the orientation direction of the fibers can be flexibly adjusted, and the generated magnetic field can be uniformly distributed and completely cover the solidification area 600. The magnetic field adjusting device 200 of the present application is directly sleeved on the outer periphery of the fixed platform device 100, so as to reduce the occupied space of the printer, and the magnetic field adjusting device 200 can rotate 360° around the fixed platform device 100, improving the convenience of controlling the direction of the magnetic field.

[0085] Embodiment 2

[0086] A fiber three-dimensional orientation printing method is adopted by using the magnetic field control three-dimensional orientation biological material 3D printer of embodiment 1.

[0087] Through the fiber three-dimensional orientation printing method, a variety of oriented 3D structure fiber products with good fiber orientation effect can be printed. After cell culture of these products, the cell orientation effect is better, as shown in Figure 14 .

[0088] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and not to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A 3D printer for biomaterials with magnetic field-controlled three-dimensional orientation, comprising a main body and a light control device, characterized in that: The system includes a fixed platform device for adhering a printing material layer with fibers that respond to changes in magnetic field, a magnetic field adjustment device for generating a magnetic field on the printing material layer, and a lifting device for raising and lowering the fixed platform device. The lifting device is fixedly mounted on the main body, and the fixed platform device is driven to the lifting device. The magnetic field adjustment device is rotatable and mounted on the outer periphery of the fixed platform device. There is a curing area between the fixed platform device and the light control device for curing and shaping the printing material layer. At least one pair of magnets of the magnetic field adjustment device are located on opposite sides of the outer periphery of the bottom surface of the curing area and can rotate 360°. The fixed platform device is provided with a cantilever, a support and an adhesion column. One end of the cantilever is connected to the lifting device for transmission, and the other end of the cantilever is fixedly connected to the support. The adhesion column is fixedly connected to the lower part of the support. The magnetic field adjustment device is connected to the cantilever and is located on the outer periphery of the adhesion column. The adhesion column is located directly above the light control device. The magnetic field adjustment device includes a drive assembly, a rotating assembly, and a piston rod for driving the magnet to rotate and keeping the magnet always located at the bottom surface of the curing area. The drive assembly is fixedly mounted on the cantilever, the rotating assembly is connected to the drive assembly, and the rotating assembly is rotatably sleeved on the outer surface of the bracket. One end of the piston rod is fixedly connected to the rotating assembly, and the other end of the piston rod is fixedly connected to the magnet. The piston rod is provided with a first sleeve, a plug and an inner rod. One end of the first sleeve is fixedly connected to the rotating assembly. One end of the inner rod is movably fitted onto the other end of the first sleeve, and the other end of the first sleeve slides against the outer surface of the inner rod. The magnet is fixedly assembled onto the other end of the inner rod. The plug is fixedly connected to one end of the inner rod, and the outer surface of the plug slides against the inner surface of the first sleeve. The rotating assembly includes a toothed ring, a rotating disk, and a second sleeve. The toothed ring is fixedly mounted on the upper outer surface of the second sleeve, the rotating disk is fixedly mounted on the lower part of the second sleeve, and the second sleeve is rotatably fitted onto the outer surface of the bracket. The magnets are provided in at least one pair, the piston rods are provided in at least two pairs, and the number of piston rods is twice the number of pistons.

2. The magnetic field-controlled three-dimensional orientation biomaterial 3D printer according to claim 1, characterized in that: The length of the magnet is not less than the maximum length of the bottom surface of the fixed platform device; The magnets are symmetrically arranged on the bottom surface of the solidification area.

3. The magnetic field-controlled three-dimensional orientation biomaterial 3D printer according to claim 1, characterized in that: The bracket is provided with a fixing rod, a pair of fixing members and a pin. The pair of fixing members are respectively fixedly connected to the opposite sides of the cantilever. The pin passes through the upper part of the fixing rod and is respectively assembled to the fixing member at both ends. The end of the pin passes through the magnetic field adjustment device and is fixedly assembled to the adhesion column.

4. The magnetic field-controlled three-dimensional orientation biomaterial 3D printer according to claim 3, characterized in that: The drive assembly includes a first motor and a gear. The first motor is fixedly mounted on the cantilever, and the gear is fixedly connected to the rotating shaft of the first motor. The gear meshes with the gear ring.

5. The magnetic field-controlled three-dimensional orientation biomaterial 3D printer according to claim 2, characterized in that: The lifting device includes a second motor, a support rod, a slide rail, a slider, a limit block, and a lead screw. One end of the support rod and the second motor are fixedly mounted to the main body. One end of the lead screw is fixedly connected to the rotating shaft of the second motor. The other ends of the support rod and the lead screw are fixedly connected to the limit block, and the support rod and the lead screw are arranged in parallel. The slide rail is fixedly mounted to one side of the support rod and is located between the support rod and the lead screw. One side of the slider is slidably connected to the slide rail, and the other side of the slider is fixedly mounted to the cantilever. The slider has an internal threaded hole in the middle, and the lead screw is fitted into the internal threaded hole.

6. The magnetic field-controlled three-dimensional orientation biomaterial 3D printer according to claim 1, characterized in that: The magnet is located on two opposite sides of the bottom surface of the solidification area; The length of the magnet is not less than the maximum length of the bottom surface of the adhesive post.

7. A method for three-dimensional fiber orientation printing, characterized in that: The process is performed using a biomaterial 3D printer with magnetic field-controlled three-dimensional orientation as described in any one of claims 1 to 6.

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