Liquid-based sample processing equipment

Abstract

The present invention discloses a liquid-based sample processing device, which includes a carrying platform, a code scanning device, a centrifugation device, a detection device, a first transfer device and a control device. The code scanning device is used to scan the sample tube and the centrifuge tube so that the coding information of the sample tube corresponds to the coding information of the centrifuge tube one by one. The centrifugation device is used to centrifuge the sample liquid in the centrifuge tube. The centrifugation device has a reference position and a detection position. The centrifuge tube can enter the centrifugation device through the reference position. The detection device is arranged in the centrifugation device and is near the traveling path of the detection position, and is used to detect the centrifuge tube located in the centrifugation device. The first transfer device is used to transfer the sample tube and the centrifuge tube, and transfer the sample liquid into the centrifuge tube and then transfer the centrifuge tube to the centrifugation device. The control device is used to obtain the detection information of the detection device and control the centrifugation device to perform a preset action on the centrifuge tube according to the detection information, so that the position of the centrifuge tube corresponds to the position of the reference position.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular, to a liquid-based sample processing device. Background Art

[0002] Liquid-based cytology examination is to detect cells and perform cytological classification diagnosis using a liquid-based thin-layer cell detection system. During the process of preparing a liquid-based sample slide, after the sample is added to a centrifuge tube containing a separating liquid, it needs to be centrifuged by a centrifugation mechanism to remove impurities from the sample. In the traditional technology, the samples after centrifugation and before centrifugation are managed and stored manually, which is likely to cause problems such as incorrect sample information recording and low efficiency. Therefore, how to effectively manage the samples before and after centrifugation is crucial. Summary of the Invention

[0003] In view of this, the present invention provides a liquid-based sample processing device.

[0004] The present invention provides a liquid-based sample processing device, including:

[0005] A carrying platform having a first sample placement area, a scanning area, and a centrifugation area, where the first sample placement area is at least used for placing sample tubes and centrifuge tubes;

[0006] A barcode scanning device disposed in the scanning area, the barcode scanning device is used to scan the sample tube and the centrifuge tube, so that the coding information of the sample tube corresponds to the coding information of the centrifuge tube one by one;

[0007] A centrifugation device disposed in the centrifugation area, the centrifugation device is used to centrifuge the sample liquid in the centrifuge tube, the centrifugation device has a reference position and a detection position, the centrifuge tube can enter the centrifugation device through the reference position, and the detection position moves synchronously with the centrifuge tube;

[0008] A detection device disposed in the centrifugation device and near the traveling path of the detection position, the detection device is used to detect the centrifuge tube located in the centrifugation device;

[0009] A first transfer device, the first transfer device is used to transfer the sample tube and the centrifuge tube between the first sample placement area and the scanning area, and transfer the sample liquid in the scanned sample tube to the scanned centrifuge tube. Before the sample liquid is transferred to the centrifuge tube, the centrifuge tube is filled with a separating liquid. After the sample liquid is transferred to the centrifuge tube, the first transfer device transfers the centrifuge tube containing the sample liquid to the centrifugation device;

[0010] A control device is configured to obtain the detection information of the detection device and control the centrifuge device to perform a preset action on the centrifuge tube according to the detection information, so that the position of the centrifuge tube corresponds to the position of the reference position.

[0011] As can be seen from the above technical solutions, in the liquid-based sample processing device of the present invention, first, the sample tube and the centrifuge tube are scanned by a barcode scanning device, so that the coding information of the sample tube corresponds to the coding information of the centrifuge tube one by one. Then, the sample in the sample tube is transferred into the centrifuge tube with the corresponding code and filled with separation liquid. Next, the centrifuge tube loaded with the separation liquid and the sample liquid is sent into the centrifuge device for centrifugation. The detection device is arranged in the centrifuge device and is used to detect the centrifuge tube located in the centrifuge device. By performing the barcode scanning operation before centrifugation, it is ensured that the sample information is accurately recorded. And through the setting of the detection device, the centrifuge tube can be detected, so that the position of the centrifuge tube after centrifugation corresponds to the position of the reference position, the grasping order of the centrifuge tubes will not be disrupted, the sample information can be corresponding one by one, and the automation operation efficiency is high, and the effective management of the samples before and after centrifugation can be realized. Description of the Drawings

[0012] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to these drawings.

[0013] Figure 1 It is a schematic structural diagram of the liquid-based sample processing device according to an embodiment of the present invention.

[0014] Figure 2 It is a structural block diagram of the liquid-based sample processing device according to an embodiment of the present invention.

[0015] Figure 3 It is a simplified schematic diagram of the control relationship between the control device and other devices according to an embodiment of the present invention.

[0016] Figure 4 It is an exploded schematic diagram of the centrifuge device according to an embodiment of the present invention.

[0017] Figure 5 It is a schematic structural diagram of the conveying mechanism according to an embodiment of the present invention.

[0018] Figure 6 It is a schematic diagram of the internal structure of the housing according to an embodiment of the present invention.

[0019] Figure 7 It is a schematic diagram when the light blocking piece does not block the optical detection unit according to an embodiment of the present invention.

[0020] Figure 8 It is a schematic diagram when the light-shielding sheet of the embodiment of the present invention shields the optical detection unit.

[0021] Figure 9 It is a simplified schematic diagram of signal transmission between the control device, the first driving mechanism and the coding unit of the embodiment of the present invention.

[0022] Figure 10 It is a schematic structural diagram of the code scanning mechanism of the embodiment of the present invention.

[0023] Figure 11 It is a partial schematic structural diagram of the first transfer device of the embodiment of the present invention.

[0024] Figure 12 It is a schematic structural diagram of the sample carrying device of the embodiment of the present invention.

[0025] Figure 13 It is an exploded schematic diagram of the sample carrying device of the embodiment of the present invention.

[0026] In the figure, 100 is a liquid-based sample processing device; 10 is a carrying platform; 11 is a first sample placing area; 12 is a scanning area; 13 is a centrifugation area; 14 is a second sample placing area; 15 is a sedimentation and staining area; 20 is a code scanning device; 21 is a receiving structure; 211 is a receiving part; 22 is a code scanning mechanism; 30 is a centrifugation device; 31 is a reference position; 32 is a housing; 321 is a centrifugation chamber; 33 is a carrier seat; 34 is a first driving mechanism; 35 is a conveying mechanism; 351 is a guiding structure; 352 is a grasping structure; 3521 is a first grasping member; 35211 is a first driving member; 35212 is a first clamping assembly; 3522 is a second grasping member; 35221 is a second driving member; 35222 is a second clamping assembly; 40 is a detection device; 41 is an optical detection unit; 42 is a light-shielding sheet; 43 is a coding unit; 50 is a first transfer device; 51 is a first material transferring mechanism; 511 is a first clamping position; 512 is a second clamping position; 52 is a second material transferring mechanism; 521 is a first plug connector; 53 is a first lifting mechanism; 54 is a second lifting mechanism; 55 is a moving seat; 60 is a control device; 70 is a coding device; 71 is a coding position; 80 is a sedimentation and staining device; 90 is a second transfer device; 110 is a separation liquid adding device; 1101 is a liquid transferring member; 1102 is a fifth lifting mechanism; 120 is an elution and mixing device; 130 is a sample carrying device; 1310 is a carrying mechanism; 1320 is a rotating mechanism; 1321 is a receiving position; 1330 is a third driving mechanism; 200 is a sample tube; 300 is a centrifuge tube; 400 is a carrier rack; 500 is a first pipette; 600 is a sedimentation tube assembly; 700 is a second pipette. Detailed implementation manners

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0028] It should also be understood that the terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in the specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include the plural forms.

[0029] It should be further understood that the term "and / or" used in the specification of the present invention and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0030] As Figures 1-3 shown, an embodiment of the present invention provides a liquid-based sample processing device 100, including a carrying platform 10, a code scanning device 20, a centrifugal device 30, a detection device 40, a first transfer device 50 and a control device 60. The carrying platform 10 has a first sample placement area 11, a scanning area 12 and a centrifugal area 13. The first sample placement area 11 is at least used for placing a sample tube 200 and a centrifuge tube 300. The code scanning device 20 is arranged in the scanning area 12, and the code scanning device 20 is used for scanning the sample tube 200 and the centrifuge tube 300 so that the coding information of the sample tube 200 corresponds to the coding information of the centrifuge tube 300 one by one. The centrifugal device 30 is arranged in the centrifugal area 13, and the centrifugal device 30 is used for centrifuging the sample liquid in the centrifuge tube 300. The centrifugal device 30 has a reference position 31 and a detection position. The centrifuge tube 300 can enter the centrifugal device 30 through the reference position 31, and the detection position moves synchronously with the centrifuge tube 300. The detection device 40 is arranged on the centrifugal device 30 and is near the traveling path of the detection position. The detection device 40 is used for detecting the centrifuge tube 300 located in the centrifugal device 30. The first transfer device 50 is used for transferring the sample tube 200 and the centrifuge tube 300 between the first sample placement area 11 and the scanning area 12, and transferring the sample liquid in the scanned sample tube 200 into the scanned centrifuge tube 300. A separation liquid is added to the centrifuge tube 300 before the sample liquid is transferred into the centrifuge tube 300. After the sample liquid is transferred into the centrifuge tube 300, the first transfer device 50 transfers the centrifuge tube 300 filled with the sample liquid to the centrifugal device 30. The control device 60 is used for obtaining the detection information of the detection device 40 and controlling the centrifugal device 30 to perform a preset action on the centrifuge tube 300 according to the detection information so that the position of the centrifuge tube 300 corresponds to the position of the reference position 31.

[0031] In the liquid-based sample processing device 100 proposed in the embodiments of the present invention, first, the sample tube 200 and the centrifuge tube 300 are scanned by the scanning device 20. After the coding information of the sample tube 200 corresponds to the coding information of the centrifuge tube 300 one by one, the sample in the sample tube 200 is transferred into the centrifuge tube 300 with corresponding coding and filled with separation liquid. Then, the centrifuge tube 300 loaded with separation liquid and sample liquid is sent into the centrifugation device 30 for centrifugation. The detection device 40 is arranged in the centrifugation device 30 and is used to detect the centrifuge tube 300 located in the centrifugation device 30. By performing the scanning operation before centrifugation, it is ensured that the sample information is accurately recorded. And through the setting of the detection device 40, the centrifuge tube 300 can be detected, so that the position of the centrifuge tube 300 after centrifugation corresponds to the position of the reference position 31. The centrifuge tube 300 is taken out in the order of first in first out, and the grasping order of the centrifuge tube 300 will not be disrupted, ensuring that the sample information can correspond one by one, and the automation operation efficiency is high, and the effective management of the sample before and after centrifugation can be realized.

[0032] In some embodiments, as Figure 4 and Figure 6 shown, the centrifugation device 30 further includes a housing 32, a carrier 33 and a first driving mechanism 34. The housing 32 has a centrifugation chamber 321, and the reference position 31 is an opening communicating with the centrifugation chamber 321. The carrier 33 is arranged in the centrifugation chamber 321, and the carrier 33 is used to carry the centrifuge tube 300 or the carrier 400 loaded with the centrifuge tube 300. The first driving mechanism 34 is connected to the carrier 33, and the first driving mechanism 34 is used to drive the carrier 33 to rotate to centrifuge the sample liquid in the centrifuge tube 300 placed on the carrier 33. The detection position is any position synchronously rotating with the centrifuge tube 300 arranged on the first driving mechanism 34 or the carrier 33, and the detection information is the position information of the centrifuge tube 300 or any point on the carrier 33. In the embodiments of the present invention, the centrifuge tube 300 or the carrier 400 placed with the centrifuge tube 300 enters the centrifugation chamber 321 through the reference position 31 and is carried on the carrier 33. The first driving mechanism 34 is used to drive the carrier 33 to rotate to centrifuge the sample liquid in the centrifuge tube 300. The detection device 40 is used to detect the position of the centrifuge tube 300 at the target position. After centrifugation, the control device 60 controls the first driving mechanism 34 to rotate to make the position of the centrifuge tube 300 at the target position correspond to the reference position 31. The centrifuge tube 300 is taken out in the order of first in first out, and the grasping order of the centrifuge tube 300 will not be disrupted, ensuring that the sample information can correspond one by one.

[0033] Exemplarily, the above-mentioned housing 32, carrier 33 and first driving mechanism 34 are assembled to form a centrifuge. The centrifuge is arranged at the bottom of the carrying platform 10 and is separately arranged from the carrying platform 10, ensuring that the vibration generated during the operation of the centrifuge will not affect the carrying platform 10.

[0034] In some embodiments, as Figure 4 and Figure 5 shown, the centrifugation device 30 further includes a transfer mechanism 35. The transfer mechanism 35 is disposed through the carrier platform 10 and is configured to receive the centrifuge tube 300 transferred by the first transfer device 50 and transfer the received centrifuge tube 300 into the centrifuge.

[0035] Exemplarily, the transfer mechanism 35 is configured to transfer a carrier 400 capable of loading the centrifuge tube 300. The first transfer device 50 transfers the centrifuge tube 300 onto the carrier 400. The transmission mechanism is configured to transfer the carrier 400 loaded with the centrifuge tube 300 to be processed into the centrifuge, and is further configured to take out the carrier 400 loaded with the centrifuge tube 300 after centrifugation processing from the centrifuge.

[0036] In some embodiments, as Figure 5 shown, the transmission mechanism includes a guiding structure 351 and a grasping structure 352. The guiding structure 351 extends linearly. The grasping structure 352 includes a first grasping member 3521 and a second grasping member 3522 connected to the guiding structure 351. Wherein, the first grasping member 3521 is configured to transfer the carrier 400 to the second grasping member 3522, or grasp the carrier 400 from the second grasping member 3522 and transfer it; the second grasping member 3522 is configured to grasp the carrier 400 from the first grasping member 3521 and transfer it, or transfer the carrier 400 from the centrifuge to the first grasping member 3521.

[0037] The transfer mechanism 35 proposed by the present invention, through the arrangement of the first grasping member 3521, the second grasping member 3522 and the guiding structure 351, can relay-transfer the carrier 400 for loading the centrifuge tube 300. The first grasping member 3521 can directly grasp the carrier 400 from the second grasping member 3522 and transfer it. Similarly, the second grasping member 3522 can also directly grasp the carrier 400 from the first grasping member 3521 and transfer it, without placing the carrier 400 at a preset position, directly realizing the transfer of the carrier 400 in the air and making the moving trajectory of the carrier 400 extend linearly along the guiding structure 351, thereby avoiding mechanical interference in the limited space of small machines.

[0038] In some embodiments, as Figure 5As shown in the figure, the first grasping member 3521 includes a first driving member 35211 and a first clamping assembly 35212. The first driving member 35211 is slidably mounted on the guiding structure 351, and the first clamping assembly 35212 is mounted on the first driving member 35211. The first driving member 35211 is configured to drive the first clamping assembly 35212 to clamp or open in the first direction to grasp or release the carrier 400. The second grasping member 3522 includes a second driving member 35221 and a second clamping assembly 35222. The second driving member 35221 is slidably mounted on the guiding structure 351, and the second clamping assembly 35222 is mounted on the second driving member 35221. The second driving member 35221 is configured to drive the second clamping assembly 35222 to clamp or open in the second direction to grasp or release the carrier 400. Herein, both the first direction and the second direction are horizontal directions and are perpendicular to each other. The lifting of the first grasping member 3521 and the second grasping member 3522 can be realized by a motor driving a transmission structure, and the transmission structure can be any one of a belt transmission mechanism 35, a chain transmission mechanism 35, a gear-rack transmission mechanism, or a lead screw transmission mechanism.

[0039] In some embodiments, as Figure 7 shown, the detection device 40 includes an optical detection unit 41 and a light shielding plate 42. The optical detection unit 41 is disposed on the inner wall of the housing 32, and the light shielding plate 42 is disposed on the carrier seat 33. The position where the light shielding plate 42 is located is the detection position. Herein, when the carrier seat 33 rotates to a position where the light shielding plate 42 corresponds to the optical detection unit 41, the light shielding plate 42 blocks the light of the optical detection unit 41, so as to trigger the optical detection unit 41 to perform electrical signal transmission, so that the rotation of the carrier 400 is recorded to detect the centrifuge tube 300 located on the carrier 400. Exemplarily, the optical detection unit 41 is a photoelectric sensor, and the position of the corresponding carrier 400 is determined by blocking the photoelectric sensor by the light shielding plate each time, so that the rotation of the carrier 400 is recorded to detect the centrifuge tube 300 located on the carrier 400.

[0040] It should be noted that the implementation manner of the detection device 40 is not limited to the above manner. For example, in some other embodiments, as Figure 8 and Figure 9 shown, the detection device 40 is connected to the output shaft of the first driving mechanism 34, and the detection device 40 includes an encoding unit 43. The encoding unit 43 is configured to record the rotation of the output shaft of the first driving mechanism 34 to detect the centrifuge tube 300 on the carrier 400 connected to the first driving mechanism 34, and the detection position is any point on the output shaft of the first driving mechanism 34.

[0041] In some embodiments, the first driving mechanism 34 includes a motor, the preset action includes the driving steps of the motor, the control device 60 calculates the angle required for the centrifuge tube 300 to rotate to a position corresponding to the position of the reference position 31 according to the detection information, and controls the motor to drive the carrier 400 to rotate so that the position of the centrifuge tube 300 corresponds to the position of the reference position 31, so as to take out the centrifuge tubes 300 in the first-in, first-out order, without disturbing the grasping order of the centrifuge tubes 300, and ensuring that the sample information can be corresponded one by one. Exemplarily, the first driving mechanism 34 is a motor, and the encoding unit 43 is an encoder connected to the motor, and the encoder is used to detect at least one of the speed (also referred to as "rotational speed" or "angular velocity", etc.) and acceleration (also referred to as "rotational acceleration" or "angular acceleration", etc.) of the motor. The control device 60 obtains the position data output from the encoder and controls the rotation of the motor according to the position data so that the position of the centrifuge tube 300 corresponds to the position of the reference position 31.

[0042] In some embodiments, as Figure 4 shown, there is one reference position 31, which is used for the centrifuge tube 300 to enter the centrifugation device 30, and for the centrifuge tube 300 that has completed the centrifugation process to be taken out from the centrifugation device 30. In this embodiment, the transmission mechanism sends the carrier 400 carrying the centrifuge tube 300 from the reference position 31 to the carrier seat 33 in the centrifugation chamber 321. After the centrifugation process is completed, the control device 60 calculates the position of the carrier 400 that was first placed in the centrifugation chamber 321 according to the detection information of the detection device 40, and controls the first driving mechanism 34 to drive the carrier 400 to rotate so that the position of the carrier 400 that was first placed in the centrifugation chamber 321 corresponds to the reference position 31, so as to facilitate the transfer mechanism 35 to take out the carrier 400 loaded with the centrifuge tube 300 that has completed the centrifugation process, take out the centrifuge tubes 300 in the first-in, first-out order, without disturbing the grasping order of the centrifuge tubes 300, and ensuring that the sample information can be corresponded one by one.

[0043] It should be noted that the number of reference positions is not limited to one. For example, in some other embodiments, at least two reference positions are provided. One of the reference positions is for the centrifuge tube to enter the centrifugal device, and the other reference position is for the centrifuge tube that has completed the centrifugation process to be taken out of the centrifugal device. In this embodiment, the transmission mechanism sends the carrier frame carrying the centrifuge tube from one of the reference positions to the carrier seat in the centrifugal chamber. After the centrifugation process is completed, the control device calculates the position of the carrier frame that was first placed in the centrifugal chamber based on the detection information of the detection device, and controls the first drive mechanism to drive the carrier frame to rotate so that the position of the carrier frame that was first placed in the centrifugal chamber corresponds to the other reference position. At this time, the centrifuge needs to move to make this reference position correspond to the position of the transmission mechanism, so that the transmission mechanism can take out the carrier frame loaded with the centrifuge tube that has completed the centrifugation process, take out the centrifuge tubes in the order of first in first out, without disrupting the grasping order of the centrifuge tubes, and ensure that the sample information can be corresponded one by one.

[0044] In some embodiments, as Figure 10 shown, the code scanning device 20 includes a housing structure 21, a second drive mechanism, and a code scanning mechanism 22. The housing structure 21 is arranged in the scanning area 12. The housing structure 21 has a housing portion 211 for housing the sample tube 200 or the centrifuge tube 300. The second drive mechanism is connected to the housing portion 211 and is used to drive the housing portion 211 to rotate. The first transfer device 50 is used to transfer the sample tube 200 and the centrifuge tube 300 to the housing portion 211 in sequence. The code scanning mechanism 22 is used to scan the sample tube 200 and the centrifuge tube 300 in sequence to obtain the coding information of the sample tube 200 and the centrifuge tube 300 and make the coding information of the sample tube 200 correspond to the coding information of the centrifuge tube 300 one by one. In this embodiment, the second drive mechanism is driven by a motor. When in use, the first transfer device 50 transfers the sample tube 200 or the centrifuge tube 300 to the housing portion 211, and the motor drives the housing portion 211 to rotate so that the code on the side wall of the sample tube 200 or the centrifuge tube 300 rotates to face the code scanner, facilitating the code scanner to perform scanning.

[0045] As an implementation manner, as Figure 10 shown, the code scanning device 20 may include two housing portions 211 and two code scanners. One of the code scanners is used to scan the sample tube 200 or the centrifuge tube 300 on one of the housing portions 211, and the other code scanner is used to scan the sample tube 200 or the centrifuge tube 300 on the other housing portion 211. By simultaneously scanning two sample tubes 200 or centrifuge tubes 300 with two code scanners, the working efficiency is improved.

[0046] As another embodiment, the code scanning device 20 may include two accommodating parts 211 and a code scanner. The code scanner can rotate to scan the sample tubes 200 or centrifuge tubes 300 on the two accommodating parts 211 successively. The two accommodating parts 211 can be driven by a driving member, reducing the volume of the liquid-based sample processing device 100 and lowering the manufacturing cost of the liquid-based sample processing device 100.

[0047] In some embodiments, as Figure 1 and Figure 11 shown, the first loading area is also used for placing the first pipette 500. The first transfer device 50 includes a first material transfer mechanism 51, a second material transfer mechanism 52, a first lifting mechanism 53, a second lifting mechanism 54, a first horizontal driving mechanism and a second horizontal driving mechanism. The first material transfer mechanism 51 has a first clamping position 511 for clamping the sample tube 200 and a second clamping position 512 for clamping the centrifuge tube 300. The second material transfer mechanism 52 has a first plug connector 521 for inserting the first pipette 500 and a first suction / discharge second driving mechanism for controlling the first pipette 500 to suck or discharge the sample liquid. The first lifting mechanism 53 is connected to the first material transfer mechanism 51, and the first lifting mechanism 53 is used to drive the first material transfer mechanism 51 to vertically lift and lower. The second lifting mechanism 54 is connected to the second material transfer mechanism 52, and the second lifting mechanism 54 is used to drive the second material transfer mechanism 52 to vertically lift and lower. The first horizontal driving mechanism is used to drive the first material transfer mechanism 51 and the second material transfer mechanism 52 to horizontally move along the first direction. The second horizontal driving mechanism is connected to the first horizontal driving mechanism, and the second horizontal driving mechanism is used to drive the first horizontal driving mechanism to horizontally move along the second direction, so as to drive the first material transfer mechanism 51 and the second material transfer mechanism 52 to horizontally move along the second direction. The first direction and the second direction are perpendicular to each other. In this embodiment, the first material transfer mechanism 51 and the second material transfer mechanism 52 can share the first horizontal driving mechanism and the second horizontal driving mechanism, saving costs. The first material transfer mechanism 51 can have the first clamping position 511 and the second clamping position 512, which can be used to adapt to the sample tube 200 and can also be used to adapt to the centrifuge tube 300, with a wide range of applications. Using the same material transfer mechanism to realize the transfer of the sample tube 200 and the centrifuge tube 300 reduces the use cost.

[0048] In some embodiments, as Figure 11 shown, a plurality of first material transfer mechanisms 51 can be provided, and a plurality of sample tubes 200 or centrifuge tubes 300 can be transferred simultaneously. A plurality of second material transfer mechanisms 52 can be provided, and a plurality of first pipettes 500 can be inserted simultaneously. The working efficiency is improved.

[0049] Exemplarily, the first lifting mechanism 53, the second lifting mechanism 54, the first horizontal driving mechanism, and the second horizontal driving mechanism all include a motor and a transmission structure connected to the motor. The transmission structure can be any one of a belt transmission mechanism 35, a chain transmission mechanism 35, a rack and pinion transmission mechanism, or a lead screw transmission mechanism.

[0050] In some embodiments, as Figure 11 shown, the first transfer device 50 further includes a moving seat 55. The first material transfer mechanism 51 and the second material transfer mechanism 52 are both slidably disposed on the moving seat 55. The first lifting mechanism 53 and the second lifting mechanism 54 are both installed on the moving seat 55. The first horizontal driving mechanism is connected to the moving seat 55. The first horizontal driving mechanism is used to drive the moving seat 55 to move horizontally along the first direction, so as to drive the first material transfer mechanism 51 and the second material transfer mechanism 52 to move horizontally along the first direction. In this embodiment, the arrangement of the moving seat 55 facilitates the first lifting mechanism 53 and the second lifting mechanism 54 to share the first horizontal driving mechanism and the second horizontal driving mechanism, saving costs.

[0051] In some embodiments, as Figure 1 and Figure 2 shown, the loading platform 10 further has a sedimentation and staining area 15. The sedimentation and staining area 15 is used to place a sedimentation tube assembly 600 containing a glass slide. The sedimentation tube assembly 600 includes a glass slide, a glass slide fixing frame, and a sedimentation tube. The glass slide is pre-loaded in the glass slide fixing frame and is clamped and fixed by the sedimentation tube to form the sedimentation tube assembly 600. The liquid-based sample processing device 100 further includes a coding device 70, a sedimentation and staining device 80, and a second transfer device 90. The coding device 70 has a coding position 71. The coding device 70 is used to code the glass slide of the sedimentation tube assembly 600 located at the coding position 71. The scanning device 20 runs synchronously with the coding device 70, so that the coding information of the glass slide corresponds one-to-one with the coding information of the centrifuge tube 300. The sedimentation and staining device 80 is disposed in the sedimentation and staining area 15. The sedimentation and staining device 80 is used to perform sedimentation and / or staining processing on the sample transferred into the sedimentation tube assembly 600. The second transfer device 90 is used to transfer the sedimentation tube assembly 600 located in the sedimentation and staining area 15 to the coding position 71 for coding and then transfer it back to the sedimentation and staining area 15, and to transfer the sample liquid in the centrifuge tube 300 after centrifugation to the corresponding coded sedimentation tube assembly 600. In the embodiments of the present invention, the sample tube 200 and the centrifuge tube 300 are scanned by the scanning device 20, so that the control device 60 can record the corresponding information. The recorded sample information is printed on the glass slide of the sedimentation tube assembly 600 by the coding device 70, so that the coding information on the sample tube 200, the centrifuge tube 300, and the glass slide corresponds one-to-one, avoiding confusion.

[0052] In some embodiments, as Figure 1 and Figure 2As shown, the loading platform 10 further has a second lofting area 14, and the second lofting area 14 is used to place the slides that have been processed and removed from the sedimentation tube assembly 600, so as to facilitate the unloading of the slides.

[0053] In some embodiments, as Figure 1 shown, the sedimentation and staining area 15 is also used to place the second pipette 700. The second transfer device 90 includes a third material transfer mechanism, a fourth material transfer mechanism, a third lifting mechanism, a fourth lifting mechanism, a third horizontal driving mechanism, and a fourth horizontal driving mechanism. The third material transfer mechanism has a second plug connector for inserting the second pipette 700 and a second suction and discharge second driving mechanism for controlling the second pipette 700 to suck or discharge the sample liquid. The fourth material transfer mechanism is used to clamp the sedimentation tube assembly 600. The third lifting mechanism is connected to the third material transfer mechanism, and the third lifting mechanism is used to drive the third material transfer mechanism to vertically lift and lower. The fourth lifting mechanism is connected to the fourth material transfer mechanism, and the fourth lifting mechanism is used to drive the fourth material transfer mechanism to vertically lift and lower. The third horizontal driving mechanism is used to drive the third material transfer mechanism and the fourth material transfer mechanism to horizontally move along the first direction; the fourth horizontal driving mechanism is connected to the third horizontal driving mechanism, and the fourth horizontal driving mechanism is used to drive the third horizontal driving mechanism to horizontally move along the second direction, so as to drive the third material transfer mechanism and the fourth material transfer mechanism to horizontally move along the second direction. The first direction and the second direction are perpendicular to each other. In this embodiment, the third material transfer mechanism and the fourth material transfer mechanism can share the third horizontal driving mechanism and the fourth horizontal driving mechanism, saving costs.

[0054] Exemplarily, the third lifting mechanism, the fourth lifting mechanism, the third horizontal driving mechanism, and the fourth horizontal driving mechanism all include a motor and a transmission structure connected to the motor. The transmission structure can be any one of a belt transmission mechanism 35, a chain transmission mechanism 35, a gear and rack transmission mechanism, or a screw transmission mechanism.

[0055] In some embodiments, as Figure 1 and Figure 11 shown, the liquid-based sample processing device 100 further includes a separation liquid filling device 110, and the separation liquid filling device 110 is used to fill the separation liquid into the centrifuge tube 300 that has been scanned and has not been filled with the sample liquid. In this embodiment, the control device 60 controls the separation liquid filling device 110 to fill the separation liquid into the empty centrifuge tube 300 located in the first loading area. At this time, the centrifuge tube 300 filled with the separation liquid can be in an inclined state through the inclined structure, and then the first transfer device 50 is controlled to suck the sample liquid from the sample tube 200 and then transfer the sample liquid to the centrifuge tube 300 filled with the separation liquid to complete the separation of the sample.

[0056] In some embodiments, as Figure 11As shown, the separation liquid filling device 110 includes a reagent container, a liquid transfer member 1101, and a pump body. The reagent container is used to store the separation liquid. The liquid transfer member 1101 is connected to the reagent container, and the pump body is connected to the reagent container. The pump body is used to drive the separation liquid in the reagent container to be output to the centrifuge tube 300 through the liquid transfer member 1101 when the liquid transfer member 1101 is directly above the centrifuge tube 300 or inside the centrifuge tube 300. In this embodiment, the liquid transfer member 1101 can be a pipette or a pipette needle or other components that can transfer liquids. The liquid transfer member 1101 can be vertically lifted and lowered by the drive of the fifth lifting mechanism 1102. The fifth lifting mechanism 1102 can be installed on the moving seat 55 of the first transfer device 50 to facilitate the three-dimensional movement of the liquid transfer member 1101, so that it moves directly above the corresponding centrifuge tube 300 or vertically inserts into the centrifuge tube 300 to avoid contact with the inner wall of the centrifuge tube 300. The pump body is used to provide driving force to make the separation liquid flow from the reagent container to the liquid transfer member 1101 and be output to the centrifuge tube 300.

[0057] In some embodiments, as Figure 1 and Figure 2 shown, the liquid-based sample processing device 100 further includes an elution and mixing device 120. The elution and mixing device 120 is used to elute the sample on the sample carrier built in the sample tube 200 into the preservation liquid in the sample tube 200 and mix the sample with the preservation liquid to form a sample solution. In this embodiment, the sample carrier is a medical swab. The elution and mixing module includes an eccentric rotation mechanism that can make the sample tube 200 rotate eccentrically to shake the sample tube 200 so that the sample on the sample carrier built in the sample tube 200 is eluted into the preservation liquid in the sample tube 200 and the sample is mixed with the preservation liquid to form a sample solution.

[0058] In some embodiments, as Figure 1 、 Figure 12 and Figure 13As shown, the liquid-based sample processing device 100 further includes a sample carrying device 130. The sample carrying device 130 includes a carrying mechanism 1310, a rotating mechanism 1320, and a third driving mechanism 1330. The carrying mechanism 1310 is movably installed on the carrying platform 10. At least a part of the carrying mechanism 1310 can be moved out of or into the first sample placing area 11. The rotating mechanism 1320 is rotatably connected to the carrying mechanism 1310. The rotating mechanism 1320 is provided with an accommodating position 1321 for storing the centrifuge tube 300 rack or the centrifuge tube 300. The third driving mechanism 1330 is used to drive the rotating mechanism 1320 to drive the centrifuge tube 300 to rotate relative to the carrying mechanism 1310, so that the centrifuge tube 300 is in an inclined state. Exemplarily, the third driving mechanism 1330 is used to drive the rotating mechanism 1320 to drive the centrifuge tube 300 to rotate relative to the carrying mechanism 1310 after the separation liquid filling device 110 fills the centrifuge tube 300 with the separation liquid, so that the centrifuge tube 300 is in an inclined state. Then, the first transfer device 50 fills the centrifuge tube 300 filled with the separation liquid and in an inclined state with the sample liquid.

[0059] The sample carrying device 130 proposed by the present invention can not only be used to place the sample tube 200, the centrifuge tube 300, and the first pipette 500, but also can keep the centrifuge tube 300 in an inclined state. When adding the sample liquid to the centrifuge tube 300 filled with the separation liquid for gradient separation, the separation effect and separation efficiency of the sample can be effectively improved. Among them, the rotating mechanism 1320 that controls the inclination of the centrifuge tube 300 is integrated with the first driving mechanism 34 in the sample carrying device 130, with a compact structure, which is beneficial to reducing the occupied space, and there is no need to separately transfer the centrifuge tube 300 to another device for inclined liquid addition operation. The inclined liquid addition is directly carried out on the carrying mechanism 1310, reducing the steps of transferring the centrifuge tube 300 and improving the working efficiency.

[0060] Exemplarily, as Figure 1 shown, multiple sample carrying devices 130 can be placed side by side in the first sample placing area 11, and more samples can be processed. In this embodiment, two sample carrying devices 130 can be stored in the first sample placing area 11.

[0061] As Figures 1-3 shown, the working process of the liquid-based sample processing device 100 according to the embodiment of the present invention is as follows:

[0062] Move the sample carrying device 130 loaded with the sample tube 200, the centrifuge tube 300, and the first pipette 500 into the first sample placing area 11.

[0063] The control device 60 controls the first transfer device 50 to successively pick up the sample tube 200 and the centrifuge tube 300 and transfer them to the barcode scanning device 20 to scan the sample tube 200 and the centrifuge tube 300, and then transfer the scanned sample tube 200 and centrifuge tube 300 back to the first sample placing area 11. The barcode scanning device 20 feeds back the relevant scanned information to the control device 60, and the control device 60 records the information so that the coding information of the sample tube 200 and the centrifuge tube 300 corresponds one by one. At this time, the control device 60 also controls the second transfer device 90 to transfer the sedimentation tube assembly 600 to the coding position 71 of the coding device 70 to print the corresponding information on the glass slide of the sedimentation tube assembly 600, so that the coding information of the sample tube 200, the centrifuge tube 300, and the glass slide corresponds one by one.

[0064] The control device 60 controls the first transfer device 50 to place the scanned sample tube 200 into the elution and mixing device 120 to mix the sample with the preservation solution to form a sample solution.

[0065] The control device 60 controls the separation liquid filling device 110 to fill the empty centrifuge tube 300 after scanning with separation liquid, and then controls the third driving mechanism 1330 to drive the rotating mechanism 1320 to drive the centrifuge tube 300 to rotate relative to the bearing mechanism 1310 so that the centrifuge tube 300 is in an inclined state, and then controls the first transfer device 50 to fill the inclined centrifuge tube 300 filled with separation liquid with the sample solution through the first pipette 500.

[0066] The control device 60 determines the bearing seat 33 corresponding to the position of the reference position 31 according to the detection information of the detection device 40, and then controls the first transfer device 50 to transfer the centrifuge tube 300 filled with separation liquid and sample solution into the carrier 400 of the transfer mechanism 35, and then controls the transmission mechanism to place the carrier 400 through the reference position 31 onto the bearing seat 33 corresponding to the reference position 31 in the centrifugation chamber 321.

[0067] The control device 60 controls the first driving mechanism 34 to drive the bearing seat 33 to rotate to centrifuge the sample solution in the centrifuge tube 300. At the same time, the detection device 40 feeds back the detection information to the control device 60. After centrifugation, the control device 60 calculates the position of the carrier 400 that was first placed in the centrifugation chamber 321 according to the detection information of the detection device 40, and controls the first driving mechanism 34 to drive the carrier 400 to rotate until the position of the carrier 400 that was first placed in the centrifugation chamber 321 corresponds to the reference position 31, so as to facilitate the transfer mechanism 35 to take out the carrier 400 loaded with the centrifuged centrifuge tube 300 and take out the centrifuge tube 300 in the order of first in first out.

[0068] The control device 60 controls the second transfer device 90 to transfer the centrifuged sample to the glass slide of the sedimentation tube assembly 600 through the second pipette 700 for sedimentation treatment, and then controls the sedimentation staining device 80 to add a staining agent to the glass slide for staining treatment.

[0069] The liquid-based sample processing device 100 of the present invention can track the entire process of sample transportation, making the information of the sample tube 200, the centrifuge tube 300, and the glass slide correspond one by one, avoiding sample confusion, and having high automation operation efficiency. It can effectively manage the samples before and after centrifugation. Among them, the control device 60 can adopt existing PLC programmable logic controllers, DDC direct digital controllers, or single-chip microcomputer control systems, etc., to implement the above control process.

[0070] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of various equivalent modifications or substitutions, and these modifications or substitutions should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. A liquid-based sample processing device, characterized in that, Comprising: A loading platform having a first layout area, a scanning area, and a centrifugation area, where the first layout area is at least used for placing sample tubes and centrifuge tubes; A barcode scanning device disposed in the scanning area, which is used to scan the sample tubes and the centrifuge tubes so that the coding information of the sample tubes corresponds one-to-one with the coding information of the centrifuge tubes; A centrifugation device disposed in the centrifugation area, which is used to centrifuge the sample liquid in the centrifuge tube. The centrifugation device has a reference position and a detection position. The centrifuge tube can enter the centrifugation device through the reference position, and the detection position moves synchronously with the centrifuge tube; A detection device disposed in the centrifugation device and near the traveling path of the detection position, which is used to detect the centrifuge tube located in the centrifugation device; A first transfer device, which is used to transfer the sample tube and the centrifuge tube between the first layout area and the scanning area, and transfer the sample liquid in the scanned sample tube to the scanned centrifuge tube. Before the sample liquid is transferred to the centrifuge tube, a separation liquid is added to the centrifuge tube. After the sample liquid is transferred to the centrifuge tube, the first transfer device transfers the centrifuge tube containing the sample liquid to the centrifugation device; A control device, which is used to obtain the detection information of the detection device and control the centrifugation device to perform a preset action on the centrifuge tube according to the detection information so that the position of the centrifuge tube corresponds to the position of the reference position; The centrifugation device further includes: A housing having a centrifugation chamber, and the reference position is an opening communicating with the centrifugation chamber; A carrier seat disposed in the centrifugation chamber, which is used to carry the centrifuge tube or a carrier rack loaded with the centrifuge tube; A first driving mechanism connected to the carrier seat, which is used to drive the carrier seat to rotate so as to centrifuge the sample liquid in the centrifuge tube placed on the carrier seat. The detection position is any position synchronously rotating with the centrifuge tube provided on the first driving mechanism or the carrier seat, and the detection information is the position information of the centrifuge tube or the position of any point on the carrier seat; The first driving mechanism includes a motor. The preset action includes the driving steps of the motor. The control device calculates the angle that the centrifuge tube needs to rotate to make its position correspond to the position of the reference position according to the detection information, and controls the motor to drive the carrier rack to rotate to make the position of the centrifuge tube correspond to the position of the reference position; The barcode scanning device includes: A housing structure disposed in the scanning area, which has a housing part for housing the sample tube or the centrifuge tube; A second driving mechanism connected to the housing part, which is used to drive the housing part to rotate; A barcode scanning mechanism, wherein the first transfer device is used to sequentially transfer the sample tube and the centrifuge tube to the accommodating part, and the barcode scanning mechanism is used to sequentially scan the sample tube and the centrifuge tube to obtain the coding information of the sample tube and the centrifuge tube and make the coding information of the sample tube correspond one-to-one with the coding information of the centrifuge tube.

2. The liquid-based sample processing device according to claim 1, characterized in that The detection device includes: An optical detection unit provided on the inner wall of the housing; A light shielding sheet provided on the carrier seat, and the position where the light shielding sheet is located is the detection position; Wherein, when the carrier seat rotates to a position where the light shielding sheet corresponds to the optical detection unit, the light shielding sheet blocks the light of the optical detection unit to trigger the optical detection unit to transmit an electrical signal, so that the rotation of the carrier frame is recorded to detect the centrifuge tube located on the carrier frame.

3. The liquid-based sample processing device according to claim 1, wherein The detection device is connected to the output shaft of the first driving mechanism, and the detection device includes a coding unit. The coding unit is used to record the rotation of the output shaft of the first driving mechanism to detect the centrifuge tube on the carrier frame connected to the first driving mechanism, and the detection position is the position of any point on the output shaft of the first driving mechanism.

4. The liquid-based sample processing device according to claim 1, wherein There is one reference position, which is used for the centrifuge tube to enter the centrifuge device and for the centrifuge tube that has completed the centrifugation process to be taken out of the centrifuge device.

5. The liquid-based sample processing device according to claim 1, characterized in that, There are at least two reference positions. One of the reference positions is used for the centrifuge tube to enter the centrifuge device, and the other reference position is used for the centrifuge tube that has completed the centrifugation process to be taken out of the centrifuge device.

6. The liquid-based sample processing device according to claim 1, wherein, The carrying platform also has a sedimentation and staining area, which is used to place the sedimentation tube assembly containing the glass slide; The liquid-based sample processing device further includes: A coding device with a coding position. The coding device is used to code the glass slide of the sedimentation tube assembly located at the coding position. The barcode scanning device and the coding device operate synchronously to make the coding information of the glass slide correspond one-to-one with the coding information of the centrifuge tube; A sedimentation and staining device provided in the sedimentation and staining area. The sedimentation and staining device is used to perform sedimentation and / or staining processing on the sample transferred into the sedimentation tube assembly; A second transfer device. The second transfer device is used to transfer the sedimentation tube assembly located in the sedimentation and staining area to the coding position for coding and then transfer it back to the sedimentation and staining area, and to transfer the sample liquid in the centrifuge tube after centrifugation processing into the corresponding coded sedimentation tube assembly.

7. The liquid-based sample processing device according to claim 1, characterized in that The liquid-based sample processing device further includes: A separation liquid filling device, which is used to fill the separation liquid into the centrifuge tube that has been scanned and has not been filled with the sample liquid.

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