High and low pressure cell culture device

Abstract

The application belongs to the technical field of cell culture equipment, and provides a high-low pressure cell culture device, which comprises a device box, a device cover movably installed on the device box, a high-pressure device cover and a low-pressure device cover, a plurality of sieve-shaped partitions for placing cell culture dishes and detachably arranged in the device box, and a first air inlet and exhaust pipe installed on the sidewall of the device box, and further comprises a pressure regulating mechanism, which comprises a pressure regulating box, a driving assembly, a first adjusting part, a fixing part, a second adjusting part and a second connecting part. The high-low pressure cell culture device can control the gas pressure in the device through the pressure regulating mechanism, completely separates the CO2 partial pressure control and temperature control of the device from the device, controls the CO2 partial pressure and temperature in the device under different pressures by controlling the CO2 concentration and ambient temperature in the high-pressure gas source, and makes the device structure simpler and the operation more convenient.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cell culture equipment, and particularly relates to a high-low pressure cell culture device. BACKGROUND

[0002] When working in special environments such as underwater, tunnels, plateaus and aerospace, the change of ambient pressure (high pressure / low pressure) can cause certain special diseases of the body, such as diving decompression sickness, aviation decompression sickness and plateau reaction, which seriously affect the work efficiency and life safety of the workers. In order to improve the work efficiency and protect the life safety of the workers, it is necessary to deeply study the pathophysiological changes and pathogenesis of these diseases and find corresponding treatment measures.

[0003] In exploring the influence of high-low pressure environment on the body and the pathogenesis of special diseases, cell culture under high-low pressure environment is needed to simulate the pathogenic conditions. In this process, the high / low pressure cell culture cabin is the basis and premise for carrying out these researches. The most important thing for cell culture is to control the environmental temperature, humidity and pH value (control the CO2 partial pressure to 5 kPa to maintain the normal pH value of the culture solution). In addition to meeting the conditions required for normal cell culture, the cell culture cabin under high / low pressure also requires good pressure resistance and air tightness.

[0004] The high-low pressure cell culture cabin needs to be specially designed and built. At present, according to the purpose, the functions of the high-low pressure cell culture cabin are divided into two categories: high pressure cell culture cabin and low pressure cell culture cabin, and the structure is relatively complex. There are few reports on cell culture cabins with high pressure and low pressure functions, which seriously restricts the research on the influence of high pressure and low pressure mixed factors on the body.

[0005] Therefore, in view of the above status, it is urgent to develop a high-low pressure cell culture device to overcome the deficiencies in current practical applications. SUMMARY

[0006] In view of the deficiencies in the prior art, the purpose of the embodiments of the present application is to provide a high-low pressure cell culture device to solve the problems in the background art.

[0007] To achieve the above purpose, the present application provides the following technical scheme:

[0008] A high-low pressure cell culture device, comprising a device box, a device cover movably installed on the device box, the device cover comprising a high pressure device cover and a low pressure device cover, a plurality of layers of cell culture dishes and detachable sieve-shaped partitions are arranged in the device box, and a first air inlet and exhaust pipe is installed on the side wall of the device box, and a valve is arranged on the first air inlet and exhaust pipe, characterized in that it further comprises:

[0009] The pressure regulating mechanism comprises a pressure regulating box, a driving assembly, a first adjusting part, a fixing part, a second adjusting part and a second connecting part, one end of the pressure regulating box extends into a device box, the driving assembly is arranged on one side in the pressure regulating box and is in threaded cooperation with a threaded groove arranged on the inner wall of the pressure regulating box, one end of the driving assembly is connected with the first adjusting part, the first adjusting part and the second adjusting part are movably arranged in the pressure regulating box, one end of the first adjusting part is connected with the second connecting part, one end of the second connecting part penetrates through the fixing part and is connected with the second adjusting part, the fixing part is fixed in the pressure regulating box, and the two ends of the fixing part are matched with the first adjusting part and the second adjusting part respectively, two ends of the inner wall of the pressure regulating box are symmetrically provided with exhaust holes, one side of the exhaust holes is connected with a second air inlet and exhaust pipe, the second air inlet and exhaust pipe is arranged on the outer wall of the pressure regulating box, and the other side of the exhaust holes is connected with the inside of the device box, and the pressure regulating mechanism realizes high and low pressure culture of cells in the device box by adjusting the pressure in the device box.

[0010] As a further technical scheme of the present application, the threaded grooves are symmetrically arranged on the inner wall of the pressure regulating box, and a 0 mark line is symmetrically arranged on the inner wall of the pressure regulating box close to the threaded grooves.

[0011] As a further technical scheme of the present application, the driving assembly comprises a control part, a first connecting part, a moving part, a mounting part and a spring, one end of the control part extends out of the pressure regulating box, the other end of the control part is fixedly connected with the moving part through the first connecting part, the moving part is movably arranged in the pressure regulating box, the outer wall of the moving part is in threaded cooperation with the threaded groove, the moving part is fixedly connected with the first adjusting part through the mounting part, the spring is movably arranged on the moving part, one end of the spring is slidably connected with the moving part, and the moving part is a telescopic structure.

[0012] As a further technical scheme of the present application, a through groove is arranged in the middle of the fixing part, the through groove is used for mounting the second connecting part, and the inner diameter of the through groove is greater than the outer diameter of the second connecting part.

[0013] As a further technical scheme of the present application, the device cover is further provided with a ventilation hole and a groove, the ventilation hole is connected with the groove, an O-ring is movably arranged in the groove, the ventilation hole on the high-pressure device cover is connected with the inside of the device box, and the ventilation hole on the low-pressure device cover is connected with the outside of the device cover.

[0014] As a further technical scheme of the present application, a fixing mechanism is movably arranged on the device box, the fixing mechanism is in contact with the device cover and the bottom of the device box respectively, and the fixing mechanism clamps and fixes the device cover and the device box.

[0015] As a further technical scheme of the present application, the fixing mechanism comprises a first fixing plate, a second fixing plate and a fixing nut, the first fixing plate is rotatably connected with the second fixing plate, the first fixing plate and the second fixing plate are provided in two groups, the two groups of the first fixing plate and the second fixing plate are respectively installed on the top of the device cover and the bottom of the device box, and the two ends of the first fixing plate and the second fixing plate are both provided with a track, one end of the fixing nut is arranged in the track, and the fixing nut is used for clamping and fixing the device cover and the device box in cooperation with the two groups of the first fixing plate and the second fixing plate.

[0016] As a further technical scheme of the present application, the bottom of the device box is further provided with a water tank.

[0017] Compared with the prior art, the present application has the following beneficial effects:

[0018] The cell culture device provided by the present application separates the two most important factors of cell culture, temperature and environmental pH value (CO2 partial pressure), from the environment and gas source for accurate control, so that the structure of the device becomes simpler, in addition to good pressure resistance and air tightness, the high and low pressure switching can also be well realized, the present application can be used for cell culture under the switching of high pressure, low pressure, normal pressure and different pressure environments, and the influence of high / low pressure environment and environmental pressure change on the physiological function of a specific cell and the mechanism thereof can be better realized;

[0019] The CO2 partial pressure control and temperature control in the device are completely independent from the device by controlling the pressure of the device, the CO2 concentration and environmental temperature in the high pressure gas source are controlled to realize the control of the CO2 partial pressure and temperature in the device under different pressures, so that the structure of the device is simpler and the operation is more convenient.

[0020] In order to more clearly set forth the structural features and effects of the present application, the present application will be described in detail below in combination with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 The structure perspective view of the high and low pressure cell culture device provided by the present application is shown.

[0022] Figure 2 The structure perspective view of the high and low pressure cell culture device provided by the present application is shown. Figure 1 The side view of the structure section at A-A in the present application is shown.

[0023] Figure 3 The structure perspective view of the high and low pressure cell culture device provided by the present application is shown. Figure 2 The structure schematic view of the pressure regulating mechanism in the present application is shown.

[0024] Figure 4 The structure perspective view of the high and low pressure cell culture device provided by the present application is shown. Figure 2 The enlarged schematic view of the structure at B on the low pressure device cover in the present application is shown.

[0025] Figure 5 For Figure 2 Enlarged schematic view of the structure at B of the high-pressure device cover.

[0026] Reference signs: 1 - device cover, 11 - air hole, 12 - groove, 2 - device box, 3 - pressure regulating mechanism, 31 - pressure regulating box, 311 - exhaust hole, 312 - threaded groove, 313 - 0 scale line, 32 - driving assembly, 321 - control piece, 322 - first connecting piece, 323 - moving piece, 324 - mounting piece, 325 - spring, 33 - first adjusting piece, 34 - fixing piece, 341 - through slot, 35 - second adjusting piece, 36 - second connecting piece, 5 - first air inlet and exhaust pipe, 51 - valve, 6 - fixing mechanism, 61 - first fixing plate, 62 - second fixing plate, 63 - fixing nut, 64 - track, 7 - partition plate, 8 - O-shaped ring, 9 - second air inlet and exhaust pipe, 10 - water tank. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0028] The specific implementation of the present application is described in detail below in combination with specific examples.

[0029] As Figures 1 to 3 shown, a high and low pressure cell culture device provided by an embodiment of the present application includes a device box 2, the device box 2 movably installs a device cover 1, the device cover 1 includes a high pressure device cover 1 and a low pressure device cover 1, a plurality of layers of cell culture dishes are placed in the device box 2 and a detachable sieve-like partition plate 7 is arranged in the device box 2, and a first air inlet and exhaust pipe 5 is installed on the side wall of the device box 2, the first air inlet and exhaust pipe 5 is provided with a valve 51, and further includes:

[0030] The pressure regulating mechanism 3 comprises a pressure regulating box 31, a driving assembly 32, a first adjusting piece 33, a fixing piece 34, a second adjusting piece 35 and a second connecting piece 36, one end of the pressure regulating box 31 extends into the device box 2, the driving assembly 32 is arranged on one side in the pressure regulating box 31 and is in threaded cooperation with a threaded groove 312 arranged on the inner wall of the pressure regulating box 31, one end of the driving assembly 32 is connected with the first adjusting piece 33, the first adjusting piece 33 and the second adjusting piece 35 are movably arranged in the pressure regulating box 31, one end of the first adjusting piece 33 is connected with the second connecting piece 36, one end of the second connecting piece 36 penetrates through the fixing piece 34 and is connected with the second adjusting piece 35, the fixing piece 34 is fixed in the pressure regulating box 31 and the two ends of the fixing piece 34 are matched with the first adjusting piece 33 and the second adjusting piece 35 respectively, two ends of the inner wall of the pressure regulating box 31 are symmetrically provided with exhaust holes 311, one side of the exhaust hole 311 is connected with the second air inlet and outlet pipe 9, the second air inlet and outlet pipe 9 is installed on the outer wall of the adjusting box 31, and the other side of the exhaust hole 311 is connected with the inside of the device box 2, the pressure regulating mechanism 3 realizes the high and low pressure culture of the device box 2 to cells by adjusting the air pressure in the device box 2.

[0031] In the embodiment, the pressure regulating mechanism 3 is used to control the gas pressure in the device, the CO2 partial pressure control and the temperature control in the device are completely separated from the device, the CO2 partial pressure and the temperature in the device under different pressures are controlled by controlling the CO2 concentration and the environmental temperature in the high pressure gas source (for example, the device is placed in a 37℃ cell culture box), the structure of the device is simpler and the operation is more convenient.

[0032] As shown in Figure 3 As a preferred embodiment of the application, the threaded groove 312 is symmetrically arranged on the inner wall of the pressure regulating box 31, the 0 mark line 313 is symmetrically arranged on the inner wall of the pressure regulating box 31 close to the threaded groove 312, one end of the driving assembly 32 is located between the two groups of 0 mark lines 313, and the second air inlet and outlet pipe 9 is further installed on the pressure regulating box 31.

[0033] As shown in Figure 3As shown in the figure, as a preferred embodiment of the present application, the driving assembly 32 comprises a control member 321, a first connecting member 322, a moving member 323, a mounting member 324 and a spring 325, one end of the control member 321 extends out of the pressure regulating box 31, and the other end of the control member 321 is fixedly connected with the moving member 323 through the first connecting member 322, the moving member 323 is movably arranged in the pressure regulating box 31, and the outer wall of the moving member 323 is screwed with the threaded groove 312, the moving member 323 is fixedly connected with the first adjusting member 33 through the mounting member 324, the spring 325 is movably sleeved on the moving member 323, one end of the spring 325 is slidably connected with the moving member 323, and the moving member 323 is a telescopic structure.

[0034] As shown in the figure, Figure 3 As shown in the figure, as a preferred embodiment of the present application, the middle part of the fixing member 34 is provided with a through groove 341 for mounting the second connecting member 36, and the inner diameter of the through groove 341 is greater than the outer diameter of the second connecting member 36.

[0035] In this embodiment, when it is needed to adjust the pressure in the device box 2 to a high pressure mode, the control member 321 is pushed, the control member 321 drives the moving member 323 to move through the first connecting member 322, the moving member 323 drives the first adjusting member 33 to move through the mounting member 324 and the spring 325, so that the first adjusting member 33 is attached to the left side of the fixing member 34, at this time the front end of the moving member 323 is aligned with the 0 mark line 313 of the positive pressure end, and at this time the moving member 323 is matched with the right side threaded groove 312, the control member 321 is rotated clockwise, so that the moving member 323 continues to move, so that the spring 325 is compressed, at this time the pressure between the first adjusting member 33 and the fixing member 34 is increased, and the increased pressure is converted into the corresponding pressure value on the scale;

[0036] When the device is pressurized by the first air inlet and exhaust pipe 5, the gas pushes the first adjusting member 33 to move reversely through the through groove between the fixing members 34, when the pressure is greater than the elastic force of the spring 325, the first adjusting member 33 is pushed away from the fixing member 34, at this time the excessive gas in the device is discharged from the second air inlet and exhaust pipe 9 through the exhaust hole on the pipe wall, the gas supply to the device is stopped, and the first air inlet and exhaust pipe 5 is closed, with the increase of the gas discharge in the device, the gas pressure in the device decreases, the force acting on the first adjusting member 33 decreases, when the force is equal to the elastic force of the spring 325, the first adjusting member 33 is attached to the fixing member 34 again, at this time the pressure in the device reaches the target pressure.

[0037] When the pressure inside the device box 2 needs to be adjusted to a low-pressure mode, pull out the control member 321, which drives the moving member 323 to move through the first connecting member 322, the moving member 323 drives the first adjusting member 33 to move through the mounting member 324 and the spring 325, the first adjusting member 33 drives the second adjusting member 35 to move through the second connecting member 36, so that the second adjusting member 35 is in close contact with the right side of the fixed member 34, at this time the left end of the moving member 323 is aligned with the 0 mark line 313 of the negative pressure end, and at this time the moving member 323 cooperates with the left threaded groove 312, rotate the control member 321 counterclockwise, at this time the spring 325 is pulled up, and the spring 325 generates a pulling force under the action of the pulling force, the second adjusting member 35 is tightly attached to the right side of the fixed member 34;

[0038] When the gas inside the device is extracted through the inlet and exhaust hole by the air pump, a negative pressure (compared to atmospheric pressure) is generated inside the device, under the action of the negative pressure, the second adjusting member 35 moves in the opposite direction of the fixed member 34, when the force generated by the negative pressure on the second adjusting member 35 is greater than the pulling force of the spring 325, the second adjusting member 35 is pulled away from the fixed member and exposes the exhaust hole 311 around the pipe wall, the gas enters the device through the through slot 341 from the second inlet and exhaust pipe 9, the air pump is turned off, as the gas enters, the negative pressure inside the device gradually decreases, when the pulling force of the negative pressure is equal to the pulling force of the spring 325, the second adjusting member 35 is again attached to the fixed member 34, at this time the pressure inside the device reaches the target negative pressure.

[0039] In a preferred embodiment, the control member 321 preferably adopts a rotating handle;

[0040] The first connecting member 322 and the second connecting member 36 both preferably adopt a rod-shaped structure;

[0041] The moving member 323, the first adjusting member 33, the fixed member 34 and the second adjusting member 35 all preferably adopt a block-shaped structure;

[0042] The mounting member 324 preferably adopts a telescopic rod-shaped structure.

[0043] As shown in Figure 4 and 5 As a preferred embodiment of the present application, the device cover 1 is also provided with a ventilation hole 11 and a recess 12, the ventilation hole 11 is in communication with the recess 12, the O-ring 8 is movably installed in the recess 12, the diameter of the O-ring 8 is slightly larger than the groove depth of the recess 12, the ventilation hole 11 on the high-pressure device cover 1 is in communication with the inside of the device box 2, and the ventilation hole 11 on the low-pressure device cover 1 is in communication with the outside of the device cover 1.

[0044] In the embodiment, when the gas in the device is extracted by the first air inlet and exhaust pipe 5 through the air pump to generate negative pressure in the device, the high air pressure of the outside world pressurizes the O-shaped ring through the air hole 11, pushes the O-shaped ring 8 into the device box 2, and increases the air tightness of the device box 2.

[0045] When pressurized, the high-pressure gas in the device enters the groove 12 through the air hole 11, pushes the O-shaped ring 8 into the device box 2, and increases the air tightness of the device box 2.

[0046] As shown in Figure 1 As a preferred embodiment of the present application, the device box 2 is further movably provided with a fixing mechanism 6, which is in contact with the top of the device cover 1 and the bottom of the device box 2, and clamps and fixes the device cover 1 and the device box 2.

[0047] As shown in Figure 1 As a preferred embodiment of the present application, the fixing mechanism 6 includes a first fixing plate 61, a second fixing plate 62 and a fixing nut 63, the first fixing plate 61 is rotatably connected with the second fixing plate 62, two groups of the first fixing plate 61 and the second fixing plate 62 are arranged on the top of the device cover 1 and the bottom of the device box 2 respectively, and tracks 64 are arranged at both ends of the first fixing plate 61 and the second fixing plate 62, the fixing nut 63 is arranged in the track 64, and the fixing nut 63 is used to clamp and fix the device cover 1 and the device box 2 in cooperation with the two groups of the first fixing plate 61 and the second fixing plate 62.

[0048] In the embodiment, the fixing nut 63 is preferably a butterfly nut, which is convenient for users to adjust the distance between the first fixing plate 61 and the second fixing plate 62.

[0049] As shown in Figure 1 As a preferred embodiment of the present application, the bottom of the device box 2 is further provided with a water tank 10.

[0050] In a preferred embodiment, the combination of the sampling pipe, the sampling valve and the flow meter arranged more finely can accurately monitor the gas environment in the device, and then the gas in the cabin can be adjusted by adjusting the CO2 concentration, the air inlet flow and the exhaust flow of the gas source, etc.

[0051] The first air inlet and exhaust pipe 5 and the air hole 11 arranged on the device cover 1 can make the gas inlet and outlet be in a diagonal position, avoid local air circulation, improve the ventilation efficiency, maximize the renewal speed of the gas in the device, and avoid the problem of poor circulation of the gas in the device caused by the close distance between the air inlet and exhaust holes. When ventilating or cleaning the inside of the device, the efficiency can be greatly improved and the time can be shortened.

[0052] A temperature-controllable gas heating mechanism can also be added, and the gas in the device is heated by a pressure-resistant metal pipe with good heat conduction, so as to well control the temperature in the device; the humidity control technology of the existing normal pressure cell incubator is used, and the humidity in the cabin is controlled by placing a distilled water tray in the device; while the cabin-penetrating parts are reserved on the side wall of the device, a probe fixing device is arranged on the top of the device cover 1, and real-time monitoring of various indexes in the cell or culture solution under high pressure can be carried out.

[0053] The above merely describes the preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A high- and low-pressure cell culture apparatus, comprising an apparatus box, wherein an apparatus cover is movably mounted on the apparatus box, the apparatus cover comprising a high-pressure apparatus cover and a low-pressure apparatus cover, wherein the apparatus box contains several layers of removable sieve-like partitions for placing cell culture dishes, and a first inlet and outlet pipe is mounted on the side wall of the apparatus box, the first inlet and outlet pipe being equipped with a valve, characterized in that, Also includes: A pressure regulating mechanism includes a pressure regulating box, a drive assembly, a first adjusting member, a fixing member, a second adjusting member, and a second connecting member. One end of the pressure regulating box extends into the device box. The drive assembly is disposed on one side inside the pressure regulating box and is threadedly engaged with a threaded groove on the inner wall of the pressure regulating box. One end of the drive assembly is connected to the first adjusting member. Both the first and second adjusting members are movably disposed within the pressure regulating box, and one end of the first adjusting member is connected to the second connecting member. One end of the second connecting member passes through the fixing member and is connected to the second adjusting member. The fixing member is fixed inside the pressure regulating box, and its two ends are respectively engaged with the first and second adjusting members. The inner wall of the pressure regulating box has symmetrically arranged exhaust holes at both ends. One exhaust hole is connected to a second inlet and outlet pipe, which is installed on the outer wall of the pressure regulating box. The other exhaust hole is connected to the interior of the device box. The pressure regulating mechanism achieves high and low pressure culture of cells in the device box by adjusting the air pressure inside the device box. A fixing mechanism is provided, which contacts the bottom of the device cover and the device box respectively, and clamps and fixes the device cover and the device box. The threaded grooves are symmetrically arranged on the inner wall of the pressure regulating box, and 0 marks are symmetrically arranged on the inner wall of the pressure regulating box close to the threaded grooves. One end of the drive component is located between the two sets of 0 marks. The drive assembly includes a control component, a first connecting component, a moving component, a mounting component, and a spring. One end of the control component extends outside the pressure regulating box, and the other end of the control component is fixedly connected to the moving component via the first connecting component. The moving component is movably disposed inside the pressure regulating box, and the outer wall of the moving component is threadedly engaged with a threaded groove. The moving component is fixedly connected to the first adjusting component via the mounting component. A spring is movably fitted on the moving component, and one end of the spring is slidably connected to the moving component. The moving component is a telescopic structure.

2. The high and low pressure cell culture device according to claim 1, characterized in that, The fastener has a through groove in the middle, which is used for the installation of the second connector, and the inner diameter of the through groove is larger than the outer diameter of the second connector.

3. The high and low pressure cell culture device according to claim 1, characterized in that, The device cover is also provided with a vent hole and a groove. The vent hole is connected to the groove. An O-ring is movably installed in the groove. The vent hole on the high-pressure device cover is connected to the inside of the device box. The vent hole on the low-pressure device cover is connected to the outside of the device cover.

4. The high and low pressure cell culture device according to claim 1, characterized in that, The fixing mechanism includes a first fixing plate, a second fixing plate, and a fixing nut. The first fixing plate and the second fixing plate are rotatably connected. There are two sets of the first fixing plate and the second fixing plate. The two sets of the first fixing plate and the second fixing plate are respectively installed on the top of the device cover and the bottom of the device box. Both ends of the first fixing plate and the second fixing plate are provided with rails. One end of the fixing nut is set in the rail. The fixing nut is used to cooperate with the two sets of the first fixing plate and the second fixing plate to clamp and fix the device cover and the device box.

5. The high and low pressure cell culture device according to claim 1, characterized in that, A water tank is also provided at the bottom of the device box.

Images