Optimized feeding method based on improved chicken feed

Abstract

An improved chicken feed-based optimized feeding method, which selects healthy laying hens in the peak egg production period, collects eggs after feeding the customized feed for 4 weeks; the improved chicken feed is composed of a base feed and a functional compound package, wherein the functional compound package is added in an amount of 1000-2000 g / t base feed; components of the functional compound package are phytase, choline chloride, silymarin, soybean phospholipid, deoxycholic acid sodium, glycine, pyrroloquinoline quinone disodium, maltodextrin, whey protein, sodium alginate, sodium hydroxide, vitamin E, organic selenium, folic acid and superfine silicon dioxide. The mutual synergy of various components in the produced eggs promotes, not only realizes nutrition strengthening and quality improvement, but also achieves the purposes of reducing blood lipids and improving fatty liver.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bioengineering, and particularly relates to an optimized feeding method based on improved chicken feed, which can realize the effects of reducing blood lipids and improving fatty liver. BACKGROUND

[0002] Eggs are deeply loved by Chinese consumers due to their high nutritional value and various cooking methods. In order to further enrich the functions of eggs, different functional components such as DHA are compounded into chicken feed, and functional eggs are prepared through the intake, digestion and biological transformation of chickens, which has a good development and application prospect.

[0003] The existing egg feed has the following shortcomings and deficiencies: the feed composition mainly meets the nutritional components required for egg production, and focuses on egg production performance, without considering increasing beneficial health components in eggs through feed regulation. After feeding the egg chickens, the functional components contained in the eggs produced are less, which cannot meet the needs of consumers in the era of health improvement through nutritionally enhanced eggs, and cannot meet the differentiated needs of consumers in the period of high-quality development of animal husbandry. SUMMARY

[0004] The present application proposes an optimized feeding method based on improved chicken feed, which not only focuses on the health and welfare of egg chickens, but also considers the metabolism and mutual cooperation of functional components in the body of egg chickens. The mutual cooperation of various components in the produced eggs promotes not only the nutrition enhancement and quality improvement, but also the reduction of blood lipids and the improvement of fatty liver.

[0005] The present application is achieved by the following technical solutions:

[0006] The present application relates to an optimized feeding method based on improved chicken feed. Healthy egg chickens in the peak egg production period are selected, and after feeding the customized feed for 4 weeks, the eggs are collected.

[0007] During the feeding process, if the egg chickens are sick and treated with drugs, all the eggs produced during the period are discarded.

[0008] The improved chicken feed is composed of a basic feed and a functional composite package, wherein: the functional composite package is added in an amount of 1000-2000g / t of the basic feed.

[0009] The components of the functional composite package are: phytase, choline chloride, silymarin, soybean phospholipid, deoxycholic acid sodium, glycine, pyrroloquinoline quinone disodium, maltodextrin, whey protein, sodium alginate, sodium hydroxide, vitamin E, organic selenium, folic acid and superfine silicon dioxide.

[0010] The component content of the functional composite package is: 4x105 -8x10 5 FTU, choline chloride 200-300g, silybin 60-80g, soybean phospholipid 30-60g, sodium deoxycholate 50-150g, glycine 60-90g, pyrroloquinoline quinone disodium 7-14g, maltodextrin 45-100g, whey protein 9-10g, sodium alginate 3.6-4g, sodium hydroxide 0.5-0.8g, vitamin E 10-30g, organic selenium selenium content 0.3-0.5g, folic acid 0.8-1.4g, superfine silicon dioxide 150-400g.

[0011] The functional complex package adopts a stainless steel mixing machine to prepare a premixed diluent, and the preparation method is as follows:

[0012] ① After dissolving sodium deoxycholate in pure water, silybin is added, then mixed with soybean phospholipid, dissolved into a clear and transparent light yellow solution in a water bath at 55°C, and then stirred at 40°C for 1.5h, and the water content of the silybin soybean phospholipid complex milk is less than 20%.

[0013] ② Dissolve whey protein and maltodextrin in pure water and stir to prepare a dispersion. Mix the silybin soybean phospholipid complex milk with the whey protein and maltodextrin dispersion, ultrasonic mixing for 15 minutes (24KHz, amplitude 50%, Hielscher UP400S), then dilute with sodium alginate dispersion, adjust the pH to 5.0, and continue to homogenize the milk emulsion with ultrasonic waves for 15 minutes. Keep the emulsion temperature below 23°C during ultrasonic emulsification, and after preparing the micro-nano lipid milk, use a vacuum dewatering machine to remove the water, so that the water content of the micro-nano lipid milk is less than 20%.

[0014] ③ Dissolve pyrroloquinoline quinone disodium in dilute sodium hydroxide solution, add superfine silicon dioxide Aerosil to the stainless steel mixing machine, then add the micro-nano lipid milk, pyrroloquinoline quinone disodium solution and other additives to the mixing machine, start mixing, supplement the share with superfine silicon dioxide while mixing, mix for 8-10 minutes to obtain a uniformly dispersed functional complex package premix.

[0015] The basic feed is corn-soybean meal type, and the specific content components are: corn 64%, soybean meal 24%, stone powder 7%, and premixed feed 5%.

[0016] The present application relates to the chicken eggs obtained by the above-mentioned method, wherein the content of choline is greater than 220mg / egg, the content of folic acid is greater than 80ug / egg, the content of vitamin E is greater than 0.7mg / egg, the content of selenium is greater than 20ug / egg, the content of silybin is greater than 1.8mg / egg, the content of pyrroloquinoline quinone is greater than 5ug / egg.

[0017] The present application relates to the chicken eggs obtained by the above-mentioned method, wherein the content of choline is greater than 220mg / egg, the content of folic acid is greater than 80ug / egg, the content of vitamin E is greater than 0.7mg / egg, the content of selenium is greater than 20ug / egg, the content of silybin is greater than 1.8mg / egg, the content of pyrroloquinoline quinone is greater than 5ug / egg.

[0018] Technical effects

[0019] Firstly, the application of sodium deoxycholate to the feed of laying hens to improve lipid metabolism and maintain the health of laying hens is novel. Sodium deoxycholate can not only improve the solubility of shuganjin in vitro, but also improve the lipid metabolism and liver health of laying hens in vivo, and can play a synergistic effect with glycine and choline, which is more conducive to the enrichment of functional ingredients. The use of deoxycholic acid to maintain the health and welfare of laying hens and to lay good eggs overcomes the shortcomings of previous other nutritionally enhanced eggs that only focus on the enrichment of functional ingredients.

[0020] Secondly, the complexing of shuganjin and phospholipids and the coating to form micro-nano liposomes to improve the deposition efficiency of shuganjin is an important innovation of the application. Shuganjin is dissolved with the help of sodium deoxycholate, and phospholipids are used as carriers to first form a complex milk, which can effectively improve the absorption and utilization of shuganjin in the body of laying hens. Then the complex milk is coated with whey protein and maltodextrin, and sodium alginate to form micro-nano lipid milk, which can further improve the absorption and utilization. The use of complex milk, nano-liposomes and other food technologies to improve the bioconversion efficiency by changing the absorption mode of active ingredients is still rare in the feed industry.

[0021] Thirdly, the innovative addition of pyrroloquinoline quinone and the increase of shuganjin content to improve the enrichment efficiency of pyrroloquinoline quinone are the substantial features of the application. Pyrroloquinoline quinone is difficult to deposit in eggs, and when added directly in the form of solid powder to feed, it will react with other ingredients, resulting in low deposition efficiency. The application uses dilute alkali solution to dissolve and disperse with fumed ultrafine silicon dioxide to improve its bioavailability in vivo. More importantly, the application makes full use of the synergistic relationship between shuganjin and pyrroloquinoline quinone to greatly improve the enrichment efficiency of pyrroloquinoline quinone. This has not been reported in previous studies.

[0022] Finally, the adsorption and slow-release properties of ultrafine silicon dioxide are also a major feature of the application. Fumed ultrafine silicon dioxide can not only protect functional ingredients from uneven mixing in feed and improve mixing uniformity, but also has a slow-release effect in the intestinal tract. Feed-grade carriers or diluents mostly use low-value products such as zeolite powder, corn starch, corn cob powder, rice husk powder and ordinary white carbon black, and there are few uses of expensive fumed ultrafine silicon dioxide. The application of nano-scale ultrafine silicon dioxide in feed premix is significantly different from the common functional compound coating of feed.

[0023] The components of the present application support and cooperate with each other, guarantee efficient enrichment of functional components and stability of active components, and belong to a typical combination application. The sodium deoxycholate not only improves the solubility of silybin in vitro, but also plays a synergistic role with glycine and choline in vivo to regulate lipid metabolism of laying hens, and is more conducive to the enrichment of functional components. Silybin can promote the deposition of pyrroloquinoline quinone, and pyrroloquinoline quinone can also play a role in reducing cholesterol and promoting the deposition of folic acid. Vitamin E and selenium have a synergistic effect, and the antioxidant properties of both can also synergistically promote the enrichment and stability of functional components. Using a single technology or certain technical means, the technical effects of the present application cannot be achieved. The simultaneous enrichment of choline, pyrroloquinoline quinone and silybin for the prevention and health care of fatty liver is also a feature and innovation of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 Schematic diagram of the appearance of the liver of the rats in the example control group and the high-fat model group;

[0025] In the figure: a is the liver of the normal group of rats, and b is the liver of the high-fat group of rats;

[0026] Figure 2 Schematic diagram of the liver tissue section of the rats in the example;

[0027] In the figure: a is the normal group, b is the fatty liver group, c is the fatty liver group + ordinary whole egg powder, and d is the fatty liver group + whole egg of the example. DETAILED DESCRIPTION

[0028] Example 1

[0029] The preparation process of the egg capable of reducing blood lipids and improving fatty liver in the present embodiment is as follows: 600 32-week-old Lohmann laying hens (laying rate 90%) are selected and randomly divided into a control group and a test group, 300 in each group, and 6 replicates are set up, 50 chickens in each replicate.

[0030] The formula and weight percentage content of the basic feedstuff for feeding the control group are as follows: corn 63%, soybean meal 27%, stone powder 8.8%, and soybean oil 1.2%.

[0031] The test group adds 1200g of functional composite package to each ton of the basic feedstuff of the control group, wherein each kilogram of the composite contains phytase (7x10 5 FTU), choline chloride (550g), silybin (60g), pyrroloquinoline quinone (12g), vitamin E (20g), organic selenium (selenium content 0.4g), and folic acid (1.2g).

[0032] After feeding for 28 days, 15 eggs were collected from each group to make a mixed egg sample, which was freeze-dried to determine the content of functional ingredients. The content of selenium in the eggs was determined by the method of GB 5009.93-2010, the content of folic acid was determined by the method of GB 5009.211, the content of choline, cholesterol, silymarin and pyrroloquinoline quinone was determined by liquid chromatography, and the content of vitamin E was determined by the method of GB 5009.82-2016.

[0033] Table 1 Content of functional ingredients in the nutritionally healthy eggs of the present example

[0034]

[0035] As shown in Table 1, the nutritionally healthy functional eggs obtained after feeding the laying hens with the feed added with the functional compound for 4 weeks have the content of various functional ingredients such as choline, pyrroloquinoline quinone, silymarin, vitamin E, folic acid and selenium effectively improved, and the content of cholesterol is reduced by 35%.

[0036] Example 2

[0037] The preparation process of the eggs for reducing blood lipid and improving fatty liver of the present example is as follows: 600 35-week-old Haiblue Brown laying hens (laying rate 92%) are selected and randomly divided into a control group and a test group, 300 in each group, and 6 replicates in each group, 50 hens in each replicate.

[0038] The control group is fed with a basic feed with the formula and weight percentage content as follows: corn 63.2%, soybean meal 27.1%, stone powder 8.5%, and soybean oil 1.2%.

[0039] The test group adds 1400 g of the functional compound to each ton of the basic feed of the control group, wherein each kilogram of the compound contains phytase (8 x 10 5 FTU), choline chloride (580 g), silymarin (70 g), pyrroloquinoline quinone (13 g), vitamin

[0040] E (22 g), organic selenium (selenium content 0.5 g), and folic acid (1.3 g).

[0041] After feeding for 28 days, 15 eggs were collected from each group to make a mixed egg sample, which was freeze-dried to determine the content of functional ingredients. The content of selenium in the eggs was determined by the method of GB 5009.93-2010, the content of folic acid was determined by the method of GB 5009.211, the content of choline, cholesterol, silymarin and pyrroloquinoline quinone was determined by liquid chromatography, and the content of vitamin E was determined by the method of GB 5009.82-2016.

[0042] Table 2 Content of functional ingredients in the nutritionally healthy eggs of the present example

[0043]

[0044] As shown in Table 2, the functional eggs with nutrition and health obtained after feeding the laying hens with the feed added with the functional compound package for 4 weeks have the effective promotion of various functional components such as choline, pyrroloquinoline quinone, silymarin, vitamin E, folic acid and selenium, and the cholesterol is reduced by 48%.

[0045] Example 3

[0046] The preparation process of the eggs with reduced blood lipid and improved fatty liver in the example is as follows: 480 Jingfen No. 6 laying hens of 39 weeks old (laying rate of 94%) are selected and randomly divided into a control group and a test group, 240 in each group, and 6 replicates are set respectively, 40 hens in each replicate.

[0047] The formula and weight percentage content of the basic feed for the control group are as follows: corn 63%, soybean meal 25%, rapeseed meal 2.4%, stone powder 8.6%, and soybean oil 1.0%.

[0048] The test group adds 1000 g of the functional compound package to each ton of the basic feed of the control group, wherein each kilogram of the compound contains phytase (6 x 10 5 FTU), choline chloride (540 g), silymarin (65 g), pyrroloquinoline quinone (12 g), vitamin

[0049] E (20 g), organic selenium (selenium content 0.5 g), and folic acid (1.1 g).

[0050] After feeding for 28 days, 15 eggs are collected from each group to make a mixed egg sample, which is freeze-dried to determine the content of the functional components. The selenium content in the eggs is determined by the method of GB 5009.93-2010, the folic acid content is determined by the method of GB 5009.211, the contents of choline, cholesterol, silymarin and pyrroloquinoline quinone are determined by liquid chromatography, and the content of vitamin E is determined by the method of GB 5009.82-2016.

[0051] Table 3 Content of functional components in the nutrition and health eggs in the example

[0052]

[0053] As shown in Table 3, the functional eggs with nutrition and health obtained after feeding the laying hens with the feed added with the functional compound package for 4 weeks have the effective promotion of various functional components such as choline, pyrroloquinoline quinone, silymarin, vitamin E, folic acid and selenium, and the cholesterol is reduced by 29%.

[0054] In view of the functional eggs obtained in Examples 1-3, the main difference is that the functional ingredient content is different, but the type is the same. Therefore, the functional eggs obtained in Example 1 are taken as an example to conduct rat animal experiments, high blood fat patient experiments, and fatty liver patient experiments to verify the effect of the eggs on reducing blood fat and improving fatty liver.

[0055] I. Fatty liver rat application verification: Construction of fatty liver model: 56 healthy SD rats were selected, and after 1 week of adaptive feeding, 48 rats were randomly selected for testing. 12 were normal groups, fed with ordinary feed, and the rest were fatty liver model groups. The rats in each group were free to drink water. High-fat feed was composed of the following raw materials: 77.5% rat basic feed, 10% egg, 10% coconut oil, 2% cholesterol, 0.5% choline salt, and 500 mg / kg / d of sodium valproate (calculated according to the weight of the rat). After 8 weeks of feeding, 1 rat from the normal group and the model group was randomly selected, and the liver was taken out as shown in Figure 1 The color of the rat liver in the normal group was dark red, the size was normal, the elasticity was good and tight, the liver of the high-fat model group rat was yellow in color, the texture was soft and easy to break, and the volume was obviously larger. Pathological sections of the liver were made, and the diagnosis was made according to the "Guidelines for the Diagnosis and Treatment of Non-alcoholic Fatty Liver Disease", and the model was confirmed to be successful.

[0056] Functional egg effect test: The ordinary eggs and the eggs of the present example collected in Example 1 were made into whole egg liquid, and after freeze-drying, they were named as ordinary whole egg powder and the whole egg powder of the present example. The ordinary egg powder and the egg powder of the present example were respectively administered to 11 test rats with successful modeling, and the normal group and the fatty liver model were used as control groups without administration. The dose was 6 g / day / kg body weight (equivalent to 1 g / kg body weight for humans, 2-3 eggs per day). It was administered every morning, and the standard daily diet was freely eaten. After 4 weeks, blood and liver samples were collected, blood lipids and liver lipids and enzyme activity content were measured, and liver samples were sectioned for observation.

[0057] By comparing and analyzing the blood lipids and transaminase components in the rat blood (as shown in Table 4), it was found that the ordinary whole egg powder group only slightly reduced cholesterol, while the whole egg powder group of the present example effectively reduced the triglycerides and total cholesterol in the blood of the fatty liver group rats, and the glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase returned to the level of the normal group, indicating that the eggs of the present example had the effect of reducing blood lipid content. The triglycerides and cholesterol in the rat liver were measured (as shown in Table 5), and the ordinary whole egg group had little effect on reducing liver triglycerides and cholesterol, while the fatty liver rats treated with the whole egg powder of the present example had similar triglyceride and cholesterol content to the normal group. In addition, through the observation of rat liver tissue sections (as shown in Figure 2Compared with the normal group, the fatty liver group and the ordinary whole egg powder group, no obvious fat granule accumulation and inflammatory lesions were found in the liver of the rats in the whole egg powder group of the embodiment. It can be seen that the functional egg prepared by the method can effectively reverse the liver fat accumulation caused by feeding high-fat feed in rats, reduce liver transaminase activity, and has good liver protection effect.

[0058] Table 4 Changes in serum lipids and transaminases of rats in each group n = 8

[0059]

[0060]

[0061] Note: In the table, the same column means that the difference is significant (P < 0.05).

[0062] Table 5 Changes in liver triglyceride and cholesterol content of rats in each group n = 8

[0063] Item Triglyceride (μmol / g) Cholesterol (μmol / g) Normal group 7.88±1.13b 2.68±0.45b Fatty liver group 18.96±3.46a 4.97±0.73a Fatty liver group + ordinary whole egg powder 16.73±2.87a 4.86±0.62a Fatty liver group + whole egg powder of the present embodiment 8.75±1.08b 2.91±0.58ab

[0064] Note: In the table, the same column means that the difference is significant (P < 0.05).

[0065] Second, application verification by high blood lipids patients and fatty liver patients: 10 high blood lipids volunteers were selected, and the fasting blood triglyceride was measured at 3.5-8.8 mmol / L and the total cholesterol was measured at 5.8-10.5 mmol / L one week before the experiment, and the drug and other health products were stopped. In the form of boiled eggs, 1-2 functional eggs of the embodiment were eaten in the morning every day, and the eating period was one month. After the experiment, the blood lipids of the patients were measured. The results showed that the blood lipids of two-thirds of the patients returned to normal, i.e. the triglyceride was lower than 1.7 mmol / L and the total cholesterol was lower than 5.2 mmol / L; the blood lipid level of one-third of the patients did not return to normal, but the decrease was more than 45%, which had a good blood lipid-lowering effect, as shown in Table 6.

[0066] Table 6 Changes in blood triglyceride and cholesterol content of high blood lipids volunteers n = 10

[0067] Item Triglyceride (μmol / L) Cholesterol (μmol / L) Before taking 6.70±1.62a 7.49±1.63a After taking for one month 1.79±0.31b 4.73±0.87b

[0068] 12 volunteers of middle-aged and young people with moderate fatty liver were recruited to verify the improvement of fatty liver. In the form of boiled eggs, according to personal preference, 1-2 eggs were taken every day, and the eating period was 2 months. The original life was maintained without intentionally increasing exercise or training. After the experiment, 10 volunteers were found to have disappeared, and 2 were converted to mild fatty liver.

[0069] Table 7 Effect of sodium deoxycholate on lipid metabolism and liver function indicators of laying hens

[0070]

[0071]

[0072] 200 45-week-old Haiblanhai laying hens were randomly assigned to four dietary treatments, and fed with basal diet and basal diet supplemented with 50, 100 and 200 mg / kg of sodium deoxycholate for 3 weeks. At the end of the experiment, 10 chickens were selected from each treatment, and the serum was separated by wing vein blood sampling to determine blood lipid and liver function indicators. As shown in Table 7, the addition of 50-150 mg / kg sodium deoxycholate in the diet can significantly reduce the content of triglyceride and total cholesterol in the blood of laying hens, and has no significant effect on the content of high-density lipoprotein cholesterol, but can significantly reduce the content of bad cholesterol (low-density lipoprotein cholesterol). In terms of liver function enzyme activity in blood, sodium deoxycholate in diet can significantly reduce the activity of lactate dehydrogenase and glutamic oxalacetic transaminase, and 100 and 150 mg / kg sodium deoxycholate can also reduce the activity of glutamic pyruvic transaminase. Therefore, the addition of sodium deoxycholate in the diet can improve the lipid metabolism of laying hens and has benefits for maintaining blood lipid and liver health.

[0073] Table 8 Effect of different processing methods of silibinin on its deposition in eggs

[0074]

[0075] 240 38-week-old Haiblanhai laying hens were randomly assigned to four dietary treatments, and fed with basal diet and basal diet supplemented with different processing forms of silibinin (80 g / t). After 4 weeks of feeding, the eggs were collected for determination of choline, cholesterol, pyrroloquinoline quinone and silibinin content. As shown in Table 8, when silibinin is directly added after dilution with starch, the silibinin content in each egg is 0.23 mg. After adding silibinin complex milk, the silibinin content reaches 0.86 mg per egg, increasing by 2.74 times. After further preparation into nano-lipid milk, the silibinin content in eggs can reach 1.83 mg, increasing by 6.96 times. In addition, the choline content in eggs increased by 48 mg per egg after using silibinin micro-nano lipid milk, increasing by 37%.

[0076] Table 9 Synergistic effect of silybin on pyrrolquinoline quinone

[0077]

[0078] 240 40-week-old Hy-Line Brown laying hens were randomly allocated to four dietary treatments, i.e., basal diet, basal diet with 10 g / t pyrrolquinoline quinone, basal diet with 80 g / t silybin, and basal diet with 10 g / t pyrrolquinoline quinone and 80 g / t silybin. After 4 weeks of feeding, egg samples were collected for determination of choline, cholesterol, pyrrolquinoline quinone, and silybin contents. As shown in Table 9, the pyrrolquinoline quinone content in each egg was 1.85 μg when the pyrrolquinoline quinone was directly added after dilution with starch, and the pyrrolquinoline quinone content was 4.23 μg when the pyrrolquinoline quinone was added after dissolution and adsorption with ultrafine silicon dioxide, which was 1.29 times higher. The pyrrolquinoline quinone content in each egg was 5.68 μg when the pyrrolquinoline quinone starch diluent was combined with silybin complex milk, indicating that silybin had a great promoting effect on the deposition of pyrrolquinoline quinone. The pyrrolquinoline quinone content in each egg was further increased (6.37 μg) when the pyrrolquinoline quinone was adsorbed with ultrafine silicon dioxide and combined with silybin complex milk. The pyrrolquinoline quinone content in each egg was continuously increased (7.12 μg) when the pyrrolquinoline quinone adsorbed with ultrafine silicon dioxide was combined with silybin complex milk nanoliposomes, which was 2.85 times higher than that of the pyrrolquinoline quinone directly added after dilution with starch. Therefore, the use of ultrafine silicon dioxide adsorption and silybin complex milk nanoliposomes can effectively achieve the fortification of pyrrolquinoline quinone in eggs.

[0079] According to the above examples and functional verification results, the egg of the present embodiment is produced by adding a customized functional complex package to the diet of laying hens in the peak laying period for a certain period of feeding. The egg is rich in beneficial functional ingredients that promote lipid metabolism in the body, and can effectively reduce triglycerides and cholesterol in the blood and liver. The functional egg is simple, convenient, and diverse to eat, and the functional ingredients for reducing blood lipids and improving fatty liver are ingeniously integrated into daily food, completely avoiding the resistance of patients to take medicine, and at the same time, achieving the purpose of prevention, health care, and even treatment.

[0080] Compared with the prior art, the present application uses laying hens as biochemical converters. It is found through egg component measurement that functional ingredients with blood lipid-lowering and liver-protecting functions such as choline, silybin, and pyrrolquinoline quinone, as well as folate, selenium, and vitamin E that promote growth and development, regulate immunity, and inhibit lipid oxidation, are all effectively enriched in eggs, and the cholesterol in the eggs is effectively reduced. Through animal experiments and clinical trials, due to the mutual synergistic promotion of the multiple components added in the egg of the present application, not only is the nutrition fortified and the quality improved, but also the purpose of reducing blood lipids and improving fatty liver is achieved.

[0081] The above specific embodiments can be partially adjusted in different ways by those skilled in the art without departing from the principles and purposes of the present application, the protection scope of the present application is subject to the claims and is not limited by the above specific embodiments, each implementation within the scope is subject to the present application.

Claims

1. An optimized feeding method based on improved chicken feed, characterized in that, Healthy laying hens at their peak egg production are selected and fed customized feed for 4 weeks before egg collection begins. The improved chicken feed consists of a basic feed and a functional compound package, wherein the amount of the functional compound package added is 1000-2000 g / t of basic feed; The components of the functional complex package are: phytase, choline chloride, silymarin, soybean lecithin, sodium deoxycholate, glycine, disodium pyrroloquinoline quinone, maltodextrin, whey protein, sodium alginate, sodium hydroxide, vitamin E, organic selenium, folic acid, and ultrafine silica. The functional composite package contains the following components: using fumed ultrafine silica as a carrier, each kilogram of composite package contains 4 × 10⁻⁶ phytase. 5 -8×10 5 FTU, choline chloride 200-300 g, silymarin 60-80 g, soybean lecithin 30-60 g, sodium deoxycholate 50-150 g, glycine 60-90 g, disodium pyrroloquinoline quinone 7-14 g, maltodextrin 45-100 g, whey protein 9-10 g, sodium alginate 3.6-4 g, sodium hydroxide 0.5-0.8 g, vitamin E 10-30 g, organic selenium 0.3-0.5 g, folic acid 0.8-1.4 g, ultrafine silica 150-400 g; The aforementioned functional composite package uses a stainless steel mixer to prepare a premixed diluent. The preparation method is as follows: ① Dissolve sodium deoxycholate in purified water, add silymarin, then mix with soybean lecithin, dissolve in a 55℃ water bath to form a clear, transparent, pale yellow solution, then stir at a constant temperature of 40℃ with a magnetic stirrer for 1.5 h, and remove water using a vacuum dryer so that the moisture content of the silymarin-soybean lecithin complex emulsion is less than 20%; ② Dissolve whey protein and maltodextrin in purified water and stir to prepare a dispersion; mix the milk thistle and soybean lecithin complex emulsion with the whey protein and maltodextrin dispersion, sonicate for 15 minutes, dilute with sodium alginate dispersion, adjust the pH to 5.0, and continue to homogenize the emulsion with ultrasound for 15 minutes; keep the emulsion temperature below 23℃ during ultrasonic emulsification, and after preparing the micro-nano lipid emulsion, remove the water with a vacuum dryer so that the water content of the micro-nano lipid emulsion is less than 20%; ③ Dissolve disodium pyrroloquinoline quinone in dilute sodium hydroxide solution, add ultrafine silica to a stainless steel mixer, then add the micro-nano lipid emulsion, disodium pyrroloquinoline quinone solution and other additives to the mixer, start mixing, and replenish the amount with ultrafine silica while mixing. Mix for 8-10 minutes to obtain a uniformly dispersed functional composite premix. The basic feed is a corn-soybean meal type, and its specific composition is: corn 64%, soybean meal 24%, limestone powder 7%, and premixed feed 5%.

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