Reagent bottles and detection systems

Abstract

The present invention discloses a reagent bottle and a detection system, wherein the reagent bottle comprises: a tube body and a tube cap, a sleeve is provided in the tube body, a sample chamber is constructed in the sleeve, a waste liquid chamber is constructed between the sleeve and the tube body, and an extraction chamber is provided at the first end of the tube body, the waste liquid chamber and the sample chamber are both connected to the extraction chamber through a microchannel, the tube cap seals the second end of the tube body, a piston chamber and a piston rod are further provided in the tube body, and the piston chamber is connected to the extraction chamber, the inner end surface of the piston rod is provided with a first puncture structure, the tube cap is movably connected to the tube body, and is suitable for pushing the piston rod to puncture the reagent contained in the piston chamber for closed tube extraction. According to the reagent bottle of an embodiment of the present invention, the tube body is closed by the tube cap, and the addition of the reagent is achieved by moving the tube cap, thereby completing the extraction and reaction under the closed tube, avoiding contamination of the sample or the environment.

Description

Technical Field

[0001] The present invention relates to the field of medical technology, and in particular to a reagent bottle and a detection system comprising the reagent bottle. Background Art

[0002] In the medical diagnostic industry, diagnosing conditions often relies on the test results of reagents. For accuracy and stability reasons, samples are typically collected via swabs, followed by extraction using reagents. Existing testing devices are bulky and require testing to be performed in varying negative pressure chambers. Furthermore, the open-ended testing method can easily affect test results and may contaminate the surrounding environment. Summary of the Invention

[0003] One purpose of the present invention is to provide a reagent bottle, which closes the tube body with a tube cap and adds reagents by moving the tube cap, thereby completing extraction and reaction under closed tube conditions and avoiding contamination of samples or the environment.

[0004] Another object of the present invention is to provide a detection system, which includes the aforementioned reagent bottle.

[0005] According to an embodiment of the present invention, a reagent bottle includes: a tube body, a sleeve is provided in the tube body, a sample chamber is constructed in the sleeve, a waste liquid chamber is constructed between the sleeve and the tube body, and an extraction chamber is provided at the first end of the tube body, the waste liquid chamber and the sample chamber are both connected to the extraction chamber through a microchannel; a tube cap, the tube cap covers the second end of the tube body, wherein a piston chamber and a piston rod are also provided in the tube body, and the piston chamber is connected to the extraction chamber, the inner end surface of the piston rod is provided with a first puncturing structure, and the tube cap is movably connected to the tube body to be suitable for pushing the piston rod to puncture the reagent contained in the piston chamber for closed-tube extraction.

[0006] According to the reagent bottle of the embodiment of the present invention, the tube body is sealed by the tube cap, and the reagent is added by moving the tube cap, thereby completing the extraction and reaction under the closed tube and avoiding contamination of the sample or the environment.

[0007] In addition, the reagent bottle according to the above embodiment of the present invention may also have the following additional technical features:

[0008] Optionally, a sealing ring is provided between the outer circumferential surface of the piston rod and the inner circumferential surface of the piston cavity.

[0009] Optionally, a first sealing groove is provided on the outer circumferential surface of the piston rod, the sealing ring is embedded in the first sealing groove, and the sealing ring protrudes from the outer circumferential surface of the piston rod.

[0010] Optionally, a second sealing groove is provided on the inner circumferential surface of the piston cavity, and the portion of the sealing ring protruding from the outer circumferential surface of the piston rod is suitable for being embedded in the second sealing groove and suitable for sliding out of the second sealing groove.

[0011] Optionally, an inner tube is provided in the tube cap, the inner tube extends along the axis of the tube body, and the tube cap is suitable for driving the inner tube to press against the piston rod.

[0012] Optionally, the tube body has a second puncture structure, and the tube cap is provided with a closed reagent cavity. The tube cap is movably connected to the tube body so as to allow the second puncture structure to puncture the reagent cavity and connect the reagent cavity with the sleeve for closed-tube extraction.

[0013] Optionally, a plurality of reagent chambers are provided at intervals in the tube cap, and the plurality of reagent chambers are suitable for being punctured in sequence by the second puncturing structure and connected to the sleeve in sequence.

[0014] Optionally, the tube cap is movably connected to the tube body along the axial direction of the tube body, and the plurality of reagent chambers are arranged at intervals along the axial direction of the tube cap.

[0015] Optionally, an inner tube is provided in the tube cap, and the inner tube extends along the axis of the tube body. The inner tube is closed at one end away from the sleeve and open at one end toward the sleeve. A plurality of diaphragms are provided in the inner tube, and the plurality of diaphragms are spaced apart along the axial direction of the tube body. The reagent chamber is constructed between each adjacent two diaphragms and between the diaphragm and the closed end of the inner tube.

[0016] Optionally, the tube cap is rotatably connected to the tube body, and the plurality of reagent chambers are arranged at intervals along the circumference of the tube cap.

[0017] Optionally, a sample preservation solution, a lysis solution, a first cleaning solution, a second cleaning solution and an air column are provided in the tube cap in the order of the puncture of the second puncture structure.

[0018] Optionally, the second puncture structure is constructed at the end of the sleeve, and the end of the sleeve is provided with a plurality of notch grooves spaced apart along the circumference of the sleeve.

[0019] Optionally, the pipe cap is threadably coupled to the pipe body.

[0020] Optionally, a sealing rib is provided on the inner end surface of the tube cap, and the sealing rib is suitable for abutting against the inner circumference of the second end of the tube body to ensure that the tube cap and the tube body are sealed together.

[0021] Optionally, an extraction membrane is provided in the extraction chamber, the waste liquid chamber is connected to the inner side of the extraction membrane, and the sample chamber is connected to the outer side of the extraction membrane.

[0022] According to an embodiment of the present invention, the detection system includes: a positioning module, a heating module, a shooting module and a reagent bottle, wherein the reagent bottle is the reagent bottle according to the aforementioned method, wherein the positioning module has an insertion port, the first end of the reagent bottle is suitable for being inserted into the insertion port and positioned by the positioning module, the heating module is used to heat the extraction chamber, and the shooting module is opposite to the end face of the first end of the reagent bottle so as to be suitable for obtaining a fluorescence quantitative image. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 Schematic diagram of a detection device according to an embodiment of the present invention.

[0024] Figure 2 This is a schematic diagram of a detection device according to an embodiment of the present invention with the casing removed.

[0025] Figure 3 It is a partial schematic diagram of a detection device according to an embodiment of the present invention.

[0026] Figure 4 It is a schematic diagram of a part of a detection device according to an embodiment of the present invention in another direction.

[0027] Figure 5 It is a schematic diagram of a mounting base and a positioning assembly of a detection device according to an embodiment of the present invention.

[0028] Figure 6 It is a schematic diagram of a mounting base, a heating element, and a hot air module of a detection device according to an embodiment of the present invention.

[0029] Figure 7 Schematic diagram of a mounting base of a detection device according to an embodiment of the present invention.

[0030] Figure 8 It is a partial schematic diagram of a detection device according to an embodiment of the present invention.

[0031] Figure 9 yes Figure 8 A schematic diagram showing the structure after removing the mounting shell.

[0032] Figure 10 It is a schematic diagram of the coordination of the turntable, probe, and filter assembly in a detection device according to an embodiment of the present invention.

[0033] Figure 11 It is a schematic diagram of the coordination of a turntable, a probe, a filter assembly, and a sensing element in a detection device according to an embodiment of the present invention.

[0034] Figure 12 It is a schematic diagram of the inner side surface of the power connection structure of the detection device according to one embodiment of the present invention.

[0035] Figure 13 Schematic diagram of a reagent bottle according to an embodiment of the present invention.

[0036] Figure 14 is a cross-sectional view of a reagent bottle according to an embodiment of the present invention.

[0037] Figure 15 yes Figure 14 A partial enlarged schematic diagram of the area A in the middle circle.

[0038] Figure 16 is a cross-sectional view of a reagent bottle according to an embodiment of the present invention.

[0039] Figure 17 Schematic diagram of a diaphragm of a reagent bottle according to an embodiment of the present invention.

[0040] Figure 18 Schematic diagram of a diaphragm of a reagent bottle according to an embodiment of the present invention.

[0041] Figure 19 Schematic diagram of a tube body of a reagent bottle according to an embodiment of the present invention.

[0042] Figure 20 Schematic diagram of a tube body of a reagent bottle according to an embodiment of the present invention.

[0043] Figure 21 Schematic diagram of a tube body of a reagent bottle according to an embodiment of the present invention.

[0044] Figure 22 Schematic diagram of the inner side of the inner end plate of a reagent bottle according to one embodiment of the present invention.

[0045] Figure 23 Schematic diagram of the outer side of the inner end plate of a reagent bottle according to one embodiment of the present invention.

[0046] Figure 24 Schematic diagram of the inner side of the outer end plate of a reagent bottle according to one embodiment of the present invention. DETAILED DESCRIPTION

[0047] The following describes embodiments of the present invention in detail, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and are not to be construed as limiting the present invention.

[0048] According to an embodiment of the present invention, the detection device 200 includes a positioning module, and an insertion port 201 is provided on the positioning module. The insertion port 201 is used for inserting the reagent bottle 100. After the reagent bottle 100 is inserted, the reagent bottle 100 can be locked, heated, heat-sealed, quantitative fluorescence detection and other operations. Among them, after the reagent bottle is inserted into the insertion port, the reagent bottle can be locked by the positioning module. The different operating devices of the reagent bottle 100 are described below with reference to the accompanying drawings.

[0049] Combine Figures 1 to 7 , describes an embodiment of a device for heating a reagent bottle 100 in the present invention, which includes multiple heating elements 22 and a driving device 23. The multiple heating elements 22 are movable to selectively heat the extraction membrane in the reagent bottle 100. That is, the multiple heating elements 22 can heat the extraction membrane in the reagent bottle 100. In general, in order to facilitate the control of the heating temperature, one or more of the multiple heating elements 22 can be used to heat the reagent bottle 100 to avoid damaging the reaction reagent, while avoiding fatigue and increasing the service life of the detection device 200. The driving device 23 is connected to the heating element 22 to drive the heating element 22 to move.

[0050] According to the detection device 200 of an embodiment of the present invention, by setting multiple heating elements 22 to selectively heat the extraction membrane in the reagent bottle 100, different detection temperatures can be met, thereby improving the applicability of the detection device 200. At the same time, it can meet the detection of the same detection target under multiple temperature conditions.

[0051] The heating element 22 in the present invention can move in multiple directions to approach and move away from the reagent bottle 100, that is, to switch between heating the reagent bottle 100 and not heating the reagent bottle 100. For example, the heating element 22 can be rotated to approach and move away from the reagent bottle 100; it can also be set to move radially along the insertion hole to approach and move away from the reagent bottle 100. In addition, the present invention also provides some embodiments to achieve heating of the reagent bottle 100.

[0052] In some embodiments of the present invention, the heating element 22 is movably disposed on the positioning module in a direction parallel to the axis of the insertion port 201. That is, the heating element 22 moves in a direction parallel to the axis of the insertion port 201 to approach and move away from the reagent bottle 100, thereby heating and not heating the reagent bottle 100. This can prevent the heating element 22 from interfering with other devices (such as the device for rotating the tube cap described below, the device for locking the tube body, etc.), improve the stability of the movement of the heating element 22, and simplify the structure of the detection device 200, thereby facilitating the design, production, and maintenance of the detection device 200.

[0053] like Figure 5The heating element 22 has a heating end for heating the reagent bottle 100. The heating end is used to approach or abut the reagent bottle 100 to heat the reagent bottle 100. The heating end is opposite to the insertion port 201 along the axis of the insertion port 201. The projections of the heating ends of the multiple heating elements 22 in the direction of the axis of the insertion port 201 are configured to form a ring with an observation port. This makes it convenient to heat the reagent bottle 100. At the same time, by providing the observation port, it is possible to avoid interference with the inspection of the reagent bottle 100 by the detection device (such as the probe 272 described below). While heating is convenient, observation is convenient, thereby improving detection efficiency.

[0054] like Figure 6 The driving device 23 includes a plurality of heating elements 22 corresponding to each other. The driving device 23 includes a heating motor and a heating cam. The heating cam is rotatably embedded in the corresponding heating element 22. The heating motor is connected to the heating cam to drive the corresponding heating element 22 to move. Specifically, a plug hole can be provided on the heating element 22. The size of the plug hole can be larger than the size of the heating cam. Since the heating cam is a non-rotating body relative to the motor shaft of the heating motor, the outer peripheral surface of the heating cam has different positions relative to the motor shaft of the heating motor, thereby realizing the driving of the heating element 22. Of course, other structures in the prior art can also be used in the present invention to realize the driving of the heating element 22.

[0055] like Figure 6 and Figure 7 The positioning module includes a mounting base 26. The heating element 22 and the driving device 23 are both arranged on the mounting base 26 and away from the insertion port 201. The mounting base 26 is provided with a matching hole 203. The heating element 22 is adapted to extend into the matching hole 203 to heat the reagent bottle 100. By providing the mounting base 26, the integration of the heating device can be facilitated, so as to facilitate the assembly and maintenance of the detection device 200, and ensure the stability of the movement of the heating element 22.

[0056] like Figure 5 The mounting seat 26 is provided with a positioning assembly, which is opposite to the insertion port 201 and is suitable for accommodating and positioning the end of the reagent bottle 100. During use, the reagent bottle is inserted from the insertion port 201, and the end of the reagent bottle is inserted into the positioning assembly, and the positioning assembly is used to position the reagent bottle, thereby fixing the tube body of the reagent bottle, thereby facilitating the rotation of the tube cap.

[0057] Among them, such as Figure 5 The positioning assembly may include a positioning ring 211 adapted to receive the end of the reagent bottle 100. During use, the end of the reagent bottle is inserted into the positioning ring 211.

[0058] Alternatively, as Figure 5, a positioning rib 212 is provided on the inner circumference of the positioning ring 211, and the positioning rib 212 extends in a direction parallel to the axis of the insertion port 201. The positioning rib 212 is used to position the reagent bottle 100 along the circumference of the insertion port 201. Correspondingly, a positioning groove is provided on the bottle body of the reagent bottle 100, and the positioning groove can be a structure adapted to the positioning rib 212. During the assembly process, the reagent bottle 100 can be inserted into the insertion port 201, and the positioning rib 212 is inserted into the positioning groove to achieve the fixation of the bottle body of the reagent bottle 100. Among them, the positioning rib 212 can be set to be asymmetric with respect to the axis of the insertion port 201. In this way, the reagent bottle 100 will be inserted into the insertion port 201 along a specific orientation. For example, when performing a heat sealing process, by limiting the orientation of the reagent bottle 100, it is convenient to insert the heat sealing needle into the reagent bottle 100 to complete the heat sealing of the corresponding opening in the reagent bottle 100.

[0059] Alternatively, as Figure 5 The positioning assembly further includes a latch 213, which is movably connected to the mounting seat 26 along the radial direction of the positioning ring 211. The latch 213 is used to position the reagent bottle 100 along the axial direction of the insertion port 201. Correspondingly, an anti-slip groove can be provided at the end of the reagent bottle 100. During use, the latch 213 can be inserted into the anti-slip groove, thereby achieving the positioning of the reagent bottle 100 along the axial direction of the insertion port 201 and preventing the reagent bottle 100 from slipping out of the insertion port 201.

[0060] In the present invention, the reagent can be added, and the reagent and sample can be mixed and reacted when the reagent bottle 100 is inserted into the detection device 200. The reagent bottle 100 can also be inserted into the detection device 200, and the actual addition can be achieved through the corresponding structure on the detection device 200.

[0061] Combine Figure 3 and Figure 4 In some embodiments of the present invention, the positioning module also includes a rotating cylinder 214 and a driving structure 215, an insertion port 201 is constructed in the rotating cylinder 214, and the rotating cylinder 214 is rotatable around the axis of the insertion port 201, and the driving structure 215 is connected with the rotating cylinder 214 to drive the rotating cylinder 214 to rotate, and the rotating cylinder 214 is suitable for locking the cap of the reagent bottle 100 to drive the cap to rotate. In use, the reagent bottle 100 is inserted into the positioning module, wherein the reagent bottle 100 includes a tube body and a cap, and by the rotation of the cap relative to the tube body, the delivery of the reagent is realized. By the positioning assembly above, the fixing of the tube body of the reagent bottle 100 can be realized, and by rotating barrel, locking the cap and rotating, namely, the rotation of the cap relative to the tube body can be realized, thereby completing the delivery of the reagent. Therefore, by arranging rotating cylinder 214 and driving structure 215, the automatic delivery of the reagent in the reagent bottle 100 can be realized.

[0062] Optionally, the heating element 22 of the present invention is a constant temperature module. The constant temperature module can be used to heat the extraction membrane in the reagent bottle 100, so as to heat the extraction membrane to a predetermined temperature to complete the detection, improve the detection effect, and avoid fatigue caused by excessive heating speed, thereby improving the structural strength and service life.

[0063] In addition, the present invention also provides some technical solutions, to realize the heat sealing of the opening in the reagent bottle 100, in some embodiments of the present invention, the detection device 200 also includes: a heat sealing device 24 and a heat sealing driver 25, the heat sealing device 24 is relative to the insertion port 201 along the axial direction of the insertion port 201, the heat sealing device 24 is movably arranged in the positioning module along the axial direction of the insertion port 201, and the heat sealing device 24 includes a heat sealing needle suitable for inserting the reagent bottle 100 and having been heat-sealed. The heat sealing driver 25 is located on the positioning module, and the heat sealing driver 25 is connected with the heat sealing device 24 to drive the heat sealing device 24 to move. After the reagent bottle 100 is inserted into the detection device 200, the heat sealing needle can be inserted into the reagent bottle 100 by moving the heat sealing device 24, thereby completing the heat sealing of the corresponding opening in the reagent bottle 100, to improve the accuracy of the detection result.

[0064] Alternatively, as Figure 6 The heat sealing drive 25 includes a heat sealing motor and a heat sealing cam. The heat sealing cam is rotatably embedded in the heat sealing device 24. The heat sealing motor is connected to the heat sealing cam to drive the heat sealing device 24 to move. The driving method of the heat sealing drive 25 is basically the same as that of the heating drive.

[0065] Optionally, in conjunction with the previous description, the positioning module includes a mounting base 26. The heat sealing device 24 is disposed on the mounting base 26, facing away from the insertion opening 201. The mounting base 26 is provided with a mating hole 203, through which the heat sealing device 24 is adapted to extend into the insertion opening 201. This facilitates the integration of the various components of the detection device 200, and facilitates assembly and maintenance. In conjunction with the previous embodiment, both the heat sealing device 24 and the heating element 22 are disposed on the mounting base 26.

[0066] like Figure 5 In some embodiments of the present invention, a limiting plate 261 is provided within the mating hole 203. The limiting plate 261 is hollow and annular, and is provided with a guide hole 262 corresponding to the heat-sealing needle. This guide hole 262 guides the heat-sealing needle and improves the stability of the heat-sealing effect. Furthermore, a guide cylinder can be provided on the limiting plate 261. The guide cylinder is a hollow cylindrical structure through which the heat-sealing cover can pass, thereby guiding the heat-sealing needle. The guide cylinder is embedded in the heating element 22, thereby guiding the heating element 22 and, consequently, guiding both the heating cylinder and the heat-sealing needle, simplifying and optimizing the structure.

[0067] In addition, the detection device 200 of the present invention also includes a device for observing the reagent bottle 100, and finally gives a reasonable and accurate conclusion by observing the reagent bottle 100 under different temperature conditions.

[0068] like Figures 8 to 12 In some embodiments of the present invention, the imaging module includes a turntable 271, a probe 272, a plurality of filter assemblies 273, and a sensing element 274. The turntable 271 is rotatably connected to the positioning module. The probe 272 is fixed relative to the positioning module and faces the end of the insertion port 201. The plurality of filter assemblies 273 are arranged at intervals around the rotation axis of the turntable 271. The plurality of filter assemblies 273 are driven by the turntable 271 to selectively face the probe 272. The sensing element 274 is configured to cooperate with the probe 272 to capture light after passing through the filter assemblies 273. During use, the reagent bottle 100 can be observed using different filter assemblies 273 by rotating the turntable 271 to meet the detection requirements and improve the accuracy of the detection results.

[0069] Among them, a sensing element 274 can be set on each filter component 273 to improve the detection efficiency. At the same time, a sensing element 274 can also be set to observe the form effect under different filter components 273. Optionally, combined with Figure 10 and Figure 11 The sensing element 274 is fixed relative to the positioning module and faces the probe 272. The multiple filter assemblies 273 are driven by the turntable 271 to selectively face the probe 272 and the sensing element 274. By fixing the sensing element 274 to the positioning module, the wiring of the sensing element 274 can be simplified, preventing the wiring harness from being entangled during the rotation of the turntable 271, effectively improving the stability of signal transmission, and at the same time, the number of sensing elements 274 can be reduced, thereby reducing costs.

[0070] Combine Figure 11 and Figure 12In some embodiments of the present invention, the turntable 271 is provided with multiple mutually insulated electrical connection structures. The electrical connection structures include: an insulating column 281, an electrical connection ring 282, and a conductive member 283. The insulating column 281 is fixed relative to the positioning module. The electrical connection ring 282 is rotatably connected to the insulating column 281. The conductive member 283 is disposed on the insulating column 281 and slidably abuts the electrical connection ring 282. The conductive member 283 is electrically connected to the electrical connection ring 282. The filter assembly 273 is electrically connected to the electrical connection ring 282. The conductive member 283 can be connected to a power source via a wiring harness. Since the insulating column 281 is fixedly connected to the positioning module, the electrical connection between the conductive member 283 and the corresponding structure on the positioning module is fixed, and the problem of wire winding will not occur. The power ring 282 can be fixedly connected to the turntable 271. Since the conductive member 283 abuts the power ring 282, the two can achieve stable electrical connection during relative movement, thereby achieving stable power connection of the conductive member 283 and further connecting the filter assembly 273 to the power supply.

[0071] In addition, an insulating ring 284 may be provided between the conductive member 283 and the turntable 271 to electrically isolate the conductive member 283 from the turntable 271 , thereby improving the safety of the power connection structure.

[0072] The insulating column 281 is a hollow structure with a through hole on its circumference. The conductive member 283 is a ball plunger disposed in the insulating column 281 and extending from the through hole. The ball head of the ball plunger abuts against the conductive member 283. This improves the stability of the conductive structure.

[0073] Alternatively, as Figure 11 The turntable 271 includes a hub 2711 and a plurality of filter seats 2712 . The plurality of filter seats 2712 are connected to the hub 2711 and are arranged around the hub 2711 . The plurality of filter assemblies 273 are matched with the plurality of filter seats 2712 in a one-to-one correspondence.

[0074] In addition, mounting grooves are provided at opposite ends of the hub 2711, and power connection structures are installed in the mounting grooves.

[0075] like Figure 8 In some embodiments of the present invention, the detection device further includes a support base 208 and a mounting shell 209. The mounting shell 209 covers the support base 208. The sensing element 274 is disposed on the support base 208, the probe 272 is disposed on the mounting shell 209, and the rotating disk 271 and the plurality of filter assemblies 273 are all disposed within the mounting shell 209. This allows for stable installation of the various components, optimizing the structure and stability.

[0076] In addition, the two insulating columns 281 of the electrical connection structure can be connected to the support base 208 and the mounting shell 209 respectively, thereby achieving a stable connection between the insulating columns 281 and the positioning module.

[0077] In addition, a circular cavity can be provided in the mounting shell 209, and the rotating disk 271 can be rotatably provided in the circular cavity. The rotating disk 271 can be positioned by the circular cavity to guide the stable rotation of the rotating disk 271.

[0078] Optionally, a rotating shaft is provided on the support base 208, and the turntable 271 includes a turntable base and a turntable cover. The turntable base is rotatably connected to the rotating shaft. The turntable base is provided with a plurality of mounting slots arranged around the rotating shaft for mounting the filter assembly 273. The turntable cover is positioned over the turntable base and covers the mounting slots. The provision of the turntable base and turntable cover prevents damage to the filter assembly 273 by other components, effectively eliminates the influence of the surrounding environment on the observation results, and improves the accuracy of the observation results.

[0079] Optionally, the turntable cover includes a base plate and multiple partitions. The base plate covers the turntable seat and is provided with holes corresponding to the multiple filter assemblies 273. The partitions are connected to the base plate and extend toward the turntable seat. The multiple partitions are spaced apart along the circumference of the base plate, with a partition provided between each adjacent filter assembly 273. The base plate allows for the packaging of multiple filter assemblies 273, while the partitions facilitate the separation of the multiple filter assemblies 273, while reducing the ingress of dust and other impurities into the filter assemblies 273, thereby improving the accuracy of the detection results.

[0080] like Figure 11 In some embodiments of the present invention, the turntable 271 further includes an outer ring gear 2713, which surrounds and is fixedly connected to the turntable base. A turntable motor is provided on the support base or the mounting housing. The turntable is driven by the turntable motor, which is in transmission connection with the outer ring gear 2713. This facilitates the rotation of the turntable 271 and improves the stability of the turntable 271 during rotation.

[0081] Combine Figures 1 to 12 In combination with the previous embodiment, the positioning module further includes a mounting seat 26, which is provided with a heating device for heating the reagent bottle 100. The mounting seat 26 is located between the turntable 271 and the insertion port 201. The mounting seat 26 is provided with a matching hole 203. The insertion port 201, the matching hole 203, and the probe 272 are relative to each other along the axis of the insertion port 201. The heating device can heat the extraction membrane, thereby obtaining good detection results. At the same time, arranging the mounting seat 26 between the turntable 271 and the insertion port 201 can simplify the structure of the heating device and the observation device. The matching hole 203 provided on the mounting seat 26 can facilitate the transmission of light to facilitate observation and improve detection efficiency.

[0082] The mounting base 26 and the supporting base 208 of the present invention can be assembled separately and then assembled together, thereby simplifying the assembly process.

[0083] like Figure 1In some embodiments of the present invention, the positioning module includes a housing 21, an insertion port 201 exposed from the outer surface of the housing 21, and a heat dissipation vent provided on the housing 21 that communicates with the interior of the housing 21. The heat dissipation vent facilitates heat dissipation of components within the positioning module, optimizing the operating environment of the various components in the detection device 200.

[0084] The positioning module, heating module and shooting module are all arranged in the casing.

[0085] Optionally, the detection device 200 further includes a display screen 29 , and the display surface of the display screen 29 is configured as a part of the appearance surface of the positioning module.

[0086] The detection device also includes a signal processing module, which is located on the positioning module and transmits signals to the sensing element 274; and a display screen 29 transmits signals to the signal processing module. By integrating the display screen 29 into the housing 21, the detection device 200 can be miniaturized and made portable.

[0087] As can be seen from the above description, the detection device 200 of the present invention can be used for PCR detection, and can improve the detection efficiency of PCR and improve the portability of the detection device 200. The detection process can be divided into the following steps:

[0088] ① The sample and the reagent are mixed in the reagent bottle 100, that is, the reagent is added to the cavity where the sample is located, and the sample is extracted and collected by the extraction membrane. This process can be completed before the reagent bottle 100 is inserted into the detection device 200, or the reagent bottle 100 can be inserted into the detection device 200, and the detection device 200 automatically completes the process of adding the reagent. The addition of the reagent and the extraction process of the extraction membrane will be described in detail below.

[0089] ② After the reagent bottle 100 is inserted into the insertion port 201, the reagent bottle 100 is locked and positioned by structures such as the latch 213 and the positioning rib 212, so that the reagent bottle 100 can be inserted into the detection device 200 according to the predetermined track and direction.

[0090] ③ After the reagent bottle 100 is inserted and the reagent is added, the reagent bottle 100 can be heat-sealed to seal the extraction chamber in the reagent bottle 100, and the actual storage reaction of multiple components is carried out in the extraction chamber as a whole, and the extraction chamber is separated from the sample chamber and the waste liquid chamber.

[0091] ④ Subsequently, the extraction membrane in the reagent bottle 100 can be heated by using the heating element 22. Since the extraction membrane and the outer end plate are relatively thin, the entire extraction membrane can be heated by heating one part of the extraction membrane. Three heating elements 22 (of course, one, two, or four or more heating elements 22) can be provided to heat the reagent bottle 100. The heating element 22 can be a constant temperature module. The heating element 22 can be raised and lowered along the axial direction of the insertion port 201 to heat or not heat the reagent bottle 100. The multiple heating elements 22 are evenly spaced around the insertion port 201. For example, the Sagan heating elements 22 are arranged at intervals of 120°. The program can be used to enable multiple heating elements 22 to heat the extraction membrane in the reagent bottle 100 respectively.

[0092] ⑤ After heating is completed or during heating, the reagent bottle 100 can be observed through the probe 272, filter assembly 273, and sensing element 274. The filter assembly 273 can be switched by rotating the turntable 271. The filter assembly 273 can be switched by continuous unidirectional rotation, shortening the wavelength switching time. The power supply can be provided by a slip-ring power supply structure. Five different colors of fluorescent filter assemblies 273 can be provided to improve detection accuracy.

[0093] Through the above steps, the detection of the reagent can be achieved and the efficiency of the detection can be improved. The present invention miniaturizes the microscope light path, has a highly sensitive color camera, and uses a colorless fluorescent filter assembly 273 to capture fluorescent images of each fluorescent channel.

[0094] In combination with the aforementioned detection device 200 , the present invention further provides a reagent bottle 100 , which can be applied to the aforementioned detection device 200 .

[0095] like Figure 13As shown, the present invention also provides a reagent bottle 100, which can be applied to the aforementioned detection equipment, and can also be produced, sold and used separately. It includes a tube body 11 and a tube cap 12, and the tube body 11 is provided with an extraction chamber, a sample chamber and a waste liquid chamber, and the sample chamber and the waste liquid chamber are both connected to the extraction chamber through a microchannel. The extraction chamber is used for reaction and extraction, the sample chamber is used for sample delivery, and the waste liquid chamber is used for collecting waste liquid. The tube cap 12 is suitable for sealing the tube body 11. At least one of the tube cap and the tube body is provided with a closed reagent chamber, and the tube cap is configured to be movable when the tube body is sealed so as to be suitable for puncturing the reagent chamber, and the reagent chamber is configured to be suitable for the reagent to flow into the sample chamber and / or the extraction chamber after puncture. Therefore, in the reagent chamber of the present invention, after the tube body is sealed with the tube cap, the reagent can still be introduced by moving the tube cap, thereby realizing closed-tube reagent introduction, closed-tube extraction and closed-tube reaction. For example, in PCR detection, sample extraction, reaction, etc. are all carried out in the detection tube, thereby effectively avoiding the influence of the external environment on the detection process, and also avoiding the generation of aerosols during the detection process to affect the surrounding environment.

[0096] In addition, in combination with the previous embodiment, after the reagent bottle 100 is inserted into the insertion port, the extraction cavity of the reagent bottle 100 can be opposite to the heating module and the shooting module, so as to realize heating and shooting of the extraction membrane of the reagent bottle 100, thereby realizing effective quantitative fluorescence detection. The process of fluorescence quantitative detection can be completed by one device, simplifying the detection.

[0097] In some embodiments of the present invention, a sleeve 111 is provided within the tube body 11. An extraction chamber is provided at the first end of the tube body 11 (refer to the lower end of the tube body 11 in the accompanying drawings). A sample chamber 1102 is constructed within the sleeve 111. A waste liquid chamber is constructed between the sleeve 111 and the tube body 11. Both the waste liquid chamber and the sample chamber are connected to the extraction chamber via a microfluidic channel. A tube cap 12 seals the second end of the tube body 11 (refer to the upper end of the tube body 11 in the accompanying drawings). A sample can be placed in the sample chamber 1102. For example, a sample swab A after collection can be placed in the sample chamber 1102. To reduce the size of the reagent bottle 100, the sample swab A, such as a cotton swab, can be broken and the effective portion placed in the sample chamber 1102. The tube cap 12 seals the tube body 11 and can move along the tube body 11. In this way, the reagent can be added by moving the tube cap 12 along the tube body 11. The present invention provides different implementations for reagent delivery. For example, the reagent can be placed in the tube cap 12 and pierced through the cannula 111. Alternatively, the reagent can be placed in the tube body 11 and delivered by moving the tube cap 12. During the reagent delivery process, the tube cap 12 seals the tube body 11, thereby preventing the generation of pollutants such as aerosols, preventing contamination of the sample and reagent in the reagent bottle, and preventing contamination of the external environment. The present invention will be described in detail.

[0098] The present invention adopts a closed reagent addition method, which can prevent contamination inside the bottle and the external environment, and reduce the impact of the external environment on the extraction and reaction process of the reagent bottle. Therefore, it can be stored and operated at moderate or room temperature.

[0099] like Figure 14 In some embodiments of the present invention, a sealed reagent chamber 1201 is provided within the tube cap 12. The tube cap 12 is movably connected to the tube body 11. The tube body further comprises a second puncturing structure, adapted to puncture the reagent chamber 1201 during movement of the tube cap 12 relative to the tube body 11, thereby connecting the reagent chamber 1201 with the cannula. This allows for reagent delivery and sample extraction to be performed while the tube cap 12 seals the tube body 11, thereby achieving closed-tube extraction. Specifically, during movement of the tube cap 12, the second puncturing structure within the tube body also moves relative to the tube cap 12 and punctures the reagent chamber 1201 within the tube cap 12. Once the reagent chamber 1201 within the tube cap 12 is punctured, the reagent chamber 1201 is connected to the sample chamber 1102, allowing the reagent within the reagent chamber 1201 to be delivered into the sample chamber 1102. The reagent within the reagent chamber 1201 can be a liquid reagent to facilitate reagent delivery.

[0100] In the present invention, there are many ways to construct the reagent chamber 1201 in the tube cap 12, for example, an air bag is set in the tube cap 12, and multiple partitions are set in the tube cap 12 to construct multiple reagent chambers 1201 in intervals. Of course, in order to facilitate the cannula 111 to puncture and connect the reagent chamber 1201, an inner tube 121 can also be set in the tube cap 12 to construct the reagent chamber 1201. The present invention is described in detail as follows.

[0101] In some embodiments of the present invention, the tube cap may have only one reagent chamber, or multiple reagent chambers may be spaced apart within the tube cap, with the multiple reagent chambers being adapted to be sequentially punctured by the second puncturing structure and subsequently connected to the cannula. When a single reagent chamber is provided within the tube cap, rapid administration of the reagent can be achieved after the chamber is punctured, while when multiple reagent chambers are provided within the tube cap, separate and sequential administration of the reagents can be achieved within the multiple chambers.

[0102] Among them, the multiple reagent chambers in the tube cap can be arranged in a variety of different ways to facilitate puncturing by the second puncturing structure.

[0103] Optionally, the tube cap is movably connected to the tube body along the axis of the tube body, and the multiple reagent chambers are spaced apart along the axial direction of the tube cap. In this case, by moving the tube cap along the axis of the tube body, the multiple reagent chambers can be sequentially punctured and connected to the sample chamber in sequence, thereby achieving the release of reagents.

[0104] like Figures 14 to 16The tube cap 12 is provided with an inner tube 121, which extends along the axis of the tube body 11. A plurality of reagent cavities 1201 are provided in the inner tube 121 at intervals along the axis of the tube body 11. The inner tube 121 is adapted to be inserted into the tube body 11 from the second end thereof, so that the sleeve 111 can sequentially pierce and connect the plurality of reagent cavities 1201. The provision of the plurality of reagent cavities 1201 in the inner tube 121 facilitates the introduction of reagents into the reagent cavities 1201. The plurality of reagent cavities 1201 are spaced apart along the axis of the tube body 11. Therefore, during the movement of the tube cap 12, the sleeve 111 can sequentially pierce the plurality of reagent cavities 1201, thereby enabling the separate introduction of reagents into the reagent cavities 1201 and meeting different testing requirements.

[0105] Optionally, combined Figures 14 to 16 The inner tube 121 is sealed at one end away from the sleeve 111. A plurality of diaphragms 122 are provided within the inner tube 121. The diaphragms 122 are spaced apart along the axis of the tube body 11. A reagent chamber 1201 is formed between each adjacent diaphragm 122 and between the diaphragm 122 and the closed end of the inner tube 121. The provision of the diaphragms 122 facilitates piercing of the sleeve 111, further facilitating piercing of the sleeve 111 and connecting the reagent chamber 1201, thereby improving the efficiency of reagent delivery.

[0106] Furthermore, if Figure 17 and Figure 18 The diaphragm 122 comprises a hard membrane layer 122a and a flexible membrane layer 122b. The hard membrane layer 122a is connected to the flexible membrane layer 122b and is connected to the flexible membrane layer 122b in a flat plate shape. The flexible membrane layer 122b is opposite the sleeve 111 along the axis of the tube body 11, and the thickness of the flexible membrane layer 122b is less than that of the hard membrane layer 122a. During use, the thinner flexible membrane layer 122b is opposite the sleeve 111. Therefore, when the tube cap 12 is removed, the sleeve 111 will easily pierce the flexible membrane layer 122b, further improving the efficiency of reagent delivery.

[0107] Optionally, the flexible film layer 122b and the hard film layer 122a are configured to form a recessed structure with an opening toward the cannula 111. During use, the end of the cannula 111 will penetrate into the recessed structure, and the recessed structure can guide the cannula 111 so that the cannula 111 can quickly and stably pierce the flexible film layer 122b. The recessed structure also provides a certain sealing effect, so that the reagent in the reagent chamber 1201 can be stably delivered into the sample chamber 1102.

[0108] Optionally, combined Figure 17 and Figure 18The surface of the flexible film layer 122b is provided with a plurality of strip grooves 122c, which are connected at one point and are configured in a divergent shape. By providing the strip grooves 122c, the flexible film layer 122b is more easily punctured, thereby improving the efficiency of the reagent delivery. Figure 17 and Figure 18 It can be seen that a strip groove 122c is provided on at least one side surface of the flexible membrane layer 122b. In addition, a plurality of annular ribs are provided on the outer surface of the outermost diaphragm 122 on the inner tube. The plurality of annular ribs can extend as a plurality of concentric circles centered on the sleeve.

[0109] Optionally, combined Figures 14 to 16 The inner tube 121 is configured as a variable diameter tube with a radial dimension gradually decreasing in a direction away from the sleeve 111, and a plurality of steps are configured on the inner circumferential surface of the inner tube 121. The septum 122 is disposed within the sleeve 111 and supported on the steps on the inner circumferential surface of the inner tube 121. In other words, the inner tube 121 is configured to include multiple tube segments, which are sequentially connected along the axis of the sleeve 111. The radial dimension of the tube segment away from the sleeve 111 is smaller than the radial dimension of the tube segment close to the sleeve 111, thereby forming a step facing the sleeve 111 between two adjacent tube segments. The septum 122 can be supported on this step. During the process of puncturing the septum 122 by the sleeve 111, the step can provide support for the septum 122, so that the sleeve 111 can stably puncture the septum 122, thereby improving the stability of the puncture process.

[0110] The inner tube 121 of the present invention can be integrally formed with the tube cap 12, or the inner tube 121 and the tube cap 12 can be separately formed. Integral forming can effectively improve the structural strength of the tube cap 12 and simplify the forming process. However, integral forming has high requirements for the mold, and the cost of the initial mold is high. Therefore, the present invention provides a structure in which the tube cap 12 and the inner tube 121 are separately formed and assembled together to simplify the production of the tube cap 12 and reduce costs. Specifically, Figure 14 and Figure 15 An annular groove is provided on the inner end surface of the tube cap 12, and an annular hook 1202 is provided on the end of the inner tube 121. The annular hook 1202 engages with the annular groove. Specifically, a rib structure can be provided on the inner end surface of the tube cap 12 to construct the annular groove. The annular groove can be configured to gradually contract inward in a direction away from the inner end surface of the tube cap 12, or the radial dimension of the annular groove can gradually decrease in a direction away from the inner end surface of the tube cap 12. A structure corresponding to the annular groove can be provided on the inner shell. For example, the annular hook 1202 on the inner shell can be configured to gradually expand outward. Through the cooperation between the annular groove and the annular hook 1202, a stable connection between the inner tube 121 and the tube cap 12 can be achieved, thereby improving the connection strength between the inner tube 121 and the tube cap 12.

[0111] In addition, in the present invention, multiple reagent cavities can also be arranged at intervals along the circumference of the tube cap, and the multiple reagent cavities can be punctured in sequence by using the second puncturing structure through the rotation of the tube cap relative to the tube body.

[0112] As mentioned above, a plurality of reagent chambers can be provided in the tube cap of the present invention, wherein the sample preservative liquid, the lysis liquid, the first cleaning liquid, the second cleaning liquid and the air column can be placed in the plurality of reagent chambers respectively, and the sample preservative liquid, the lysis liquid, the first cleaning liquid, the second cleaning liquid and the air column are arranged in sequence to achieve separate addition. The sample preservative liquid, the lysis liquid, the first cleaning liquid, the second cleaning liquid and the air column are arranged in sequence in the tube cap along the puncture sequence of the second puncture structure. For example, in an embodiment in which the plurality of reagent chambers are arranged at intervals along the axial direction of the tube body, the sample preservative liquid, the lysis liquid, the first cleaning liquid, the second cleaning liquid and the air column can be arranged in sequence in the plurality of reagent chambers arranged along the axial direction of the tube body, and the reagent chamber for storing the sample preservative liquid is close to the opening of the tube cap, so that the sample preservative liquid is first put into the sample chamber.

[0113] In the present invention, the end of the sleeve can be constructed into the aforementioned second puncture structure, thereby simplifying the tube body.

[0114] Among them, combined Figure 14 and Figure 21 In order to further facilitate the sleeve 111 to puncture the reagent chamber 1201, a plurality of notched grooves 1103 can be provided at the end of the sleeve 111 at intervals along the circumference of the sleeve 111. In this way, when the sleeve 111 punctures the reagent chamber 1201, the side edges of the notched grooves 1103 can provide the sleeve 111 with multiple puncture points, thereby improving the puncture efficiency. In addition, the tube cap 12 of the present invention can be configured to be threadedly connected to the tube body 11, and the sleeve 111 can be punctured into the reagent chamber 1201 by rotating the nut. During this process, the rotation of the tube cap 12 allows the sleeve 111 to rotatably puncture the reagent chamber 1201. By providing the notched grooves 1103, the force applied by the circumference of the notched grooves 1103 to the reagent chamber 1201 will have a good stress concentration phenomenon, thereby achieving rapid puncture of the reagent chamber 1201.

[0115] like Figure 14In some embodiments of the present invention, a sealing rib 1204 is provided on the inner end surface of the tube cap 12. The sealing rib 1204 is adapted to abut against the inner circumference of the second end of the tube body 11, thereby ensuring a sealed fit between the tube cap 12 and the tube body 11. By providing the sealing rib 1204, when the tube cap 12 is moved to a predetermined position along the axis of the tube body 11, for example, when the end of the tube body 11 abuts against the inner end surface of the tube cap 12, the sealing rib 1204 will abut against the inner circumference of the tube body 11, thereby achieving a seal between the tube cap 12 and the tube body 11, preventing leakage of the reagent in the reagent bottle 100, improving the accuracy of the test results, and avoiding waste.

[0116] In the present invention, the tube cap 12 can be moved axially along the tube body 11 in a straight line direction. Of course, other methods can also be used to achieve the movement of the tube cap 12 relative to the tube body 11. Preferably, in one embodiment of the present invention, the tube cap 12 is threadedly connected to the tube body 11, which not only facilitates the stable connection between the tube cap 12 and the tube body 11, but also enables the movement of the tube cap 12 relative to the tube body 11 through threaded transmission. In addition, the threaded transmission can enable the sleeve 111 to have a greater force to puncture the reagent cavity 1201, thereby improving the stability of the structure and the efficiency of reagent delivery. Figures 14 to 16 In some embodiments of the present invention, the pipe cap 12 is sleeved onto the outside of the pipe body 11. The inner circumference of the pipe cap 12 is provided with a first coarse thread 1205, and the outer circumference of the pipe body 11 is provided with a second coarse thread 1104. The first coarse thread 1205 and the second coarse thread 1104 cooperate with each other. The cooperation between the first coarse thread 1205 and the second coarse thread 1104 can achieve a stable connection between the pipe cap 12 and the pipe body 11.

[0117] The outer circumferential surface of the tube body 11 is also provided with a fine thread, which cooperates with the second coarse thread 1104, and the first coarse thread 1205 contacts the fine thread when the sleeve 111 punctures the reagent chamber 1201. Through the cooperation of the first coarse thread 1205 and the second coarse thread 1104, the threaded cooperation of the tube cap 12 and the tube body 11 can be achieved, and the fine thread provided on the tube body 11 can enhance the resistance to rotating the tube cap 12. When manually rotating the tube cap 12, appropriate feedback can be given to the user to remind the user. The setting position of the fine thread in the present application can increase the resistance to the tube cap 12 during the process of the sleeve 111 puncturing the reagent chamber 1201, so as to remind the user to use it.

[0118] In addition, as mentioned above, the tube cap 12 of the present invention is provided with multiple reagent chambers 1201. By providing fine threads, it is convenient to remind the user that if the types of reagents required are less than the actual number of reagent chambers 1201, the user can discover and control it in time.

[0119] That is to say, during the rotation of the tube cap 12 in the present invention, when the wall of the reagent chamber 1201 in the tube cap 12 (such as the diaphragm 122 mentioned above) begins or is about to begin to touch the sleeve 111, the first coarse thread 1205 will cooperate with the second coarse thread 1104 and the fine thread, thereby giving timely feedback to the user, making it convenient for the user to understand the working status of the reagent bottle 100 and to make adjustments.

[0120] Optionally, combined Figure 16 、 Figure 22-Figure 24 The first end of the tube body 11 is provided with an inner end plate 112 and an outer end plate 113. An extraction chamber is constructed between the inner end plate 112 and the outer end plate 113. A first through hole 1106 is constructed on the inner end plate 112, and the first through hole 1106 connects the sample chamber 1102 and the extraction chamber. By providing the inner end plate 112 and the outer end plate 113, the first end of the tube body 11 can be easily sealed, and the structure of the extraction chamber is simplified, which facilitates the molding of the tube body 11. In addition, an extraction membrane 118 can be placed in the extraction chamber, and the extraction membrane 118 can be clamped by the inner end cap and the outer end cap, thereby improving the structural strength and stability of the reagent bottle 100.

[0121] The first through hole and the second through hole are suitable for being heat-sealed by a heat-sealing device. Specifically, after the reagent bottle is inserted into the insertion port, a heat-sealing needle can be inserted through the outer end plate into the inner end plate to seal the first through hole and the second through hole on the inner end plate.

[0122] Furthermore, to facilitate heating and observation of the reagent bottle, the reagent bottle can be locked by the positioning module in the aforementioned embodiment, wherein the positioning module can be used to lock the tube body and tube cap of the reagent bottle, and the positioning module can be used to drive the tube cap to move relative to the tube body to achieve the reagent delivery operation. For example, the positioning module can be used to drive the tube cap to rotate relative to the tube body.

[0123] Optionally, combined Figure 13 and Figure 19 An anti-slip groove 1107 is provided on the outer circumferential surface of the first end of the tube body 11. The anti-slip groove 1107 is arranged along the circumference of the tube body 11. The anti-slip groove 1107 can be annular and extend along the circumference of the tube body 11, or can be a groove extending intermittently along the circumference of the tube body 11. When the tube body 11 is placed in the detection equipment, the corresponding structure on the detection equipment can achieve axial positioning of the tube body 11 to prevent the reagent bottle 100 from falling out of the detection equipment.

[0124] In combination with the previous embodiment, the positioning module is suitable for cooperating with the anti-slip groove to position the reagent bottle along the axial direction of the insertion port, wherein the pin in the previous embodiment can be inserted into the anti-slip groove to also lock the reagent bottle.

[0125] In addition, an anti-dropout protrusion or the like may be provided on the outer peripheral surface of the first end of the tube body 11 , and the axial positioning of the reagent bottle 100 may also be achieved through the anti-dropout protrusion or the like.

[0126] Optionally, combined Figure 19 A positioning groove 1108 can also be provided on the outer peripheral surface of the first end of the tube body 11. The positioning groove 1108 can extend in a direction parallel to the axis of the tube body 11. During use, positioning ribs can be provided at corresponding positions of the detection equipment to achieve circumferential positioning of the tube body 11.

[0127] The circumferential positioning of the tube body can be achieved by the aforementioned positioning module. For example, the aforementioned positioning ribs are embedded in the positioning grooves to achieve circumferential locking of the tube body.

[0128] Alternatively, as Figure 13 The outer circumference of the tube cap 12 is provided with a plurality of tooth grooves 1206. The tooth grooves 1206 are parallel to the axis of the tube body 11 and are spaced apart along the circumference of the tube cap 12. The plurality of tooth grooves 1206 can increase the friction force on the outer circumference of the tube cap 12, thereby improving the stability of the reagent bottle 100 when the tube cap 12 is manually or automatically driven to move along the axis of the tube body 11.

[0129] The tooth groove 1206 can be used to improve the stability of manual or motorized rotation of the tube cap 12. For example, the positioning module can cooperate with the tooth groove to achieve circumferential locking of the tube cap, and then the reagent can be added by rotating the tube cap.

[0130] The tooth grooves 1206 on the outer peripheral surface of the tube cap 12 can be arranged to extend in a direction parallel to the axis of the tube body 11 .

[0131] Alternatively, as Figure 13 and Figure 19 The outer circumferential surface of the first end of the tube body 11 is provided with anti-slip ribs 1109. The anti-slip ribs 1109 extend in a direction parallel to the axis of the tube body 11, and multiple anti-slip ribs 1109 are provided at intervals along the circumference of the tube body 11. The anti-slip ribs 1109 can increase the friction of the tube body 11. During use, when rotating the tube cap 12, force needs to be applied to both the tube cap 12 and the tube body 11. Therefore, by providing the teeth 1206 on the tube cap 12 and the anti-slip ribs 1109 on the tube body 11, the rotation of the tube cap 12 relative to the tube body 11 can be facilitated.

[0132] Of course, other structures can also be provided in the present invention to increase the friction between the outer peripheral surface of the pipe cap 12 and the outer peripheral surface of the tube body 11. For example, groove-shaped, protrusion-shaped or other anti-slip structures can be provided on the outer peripheral surface of the pipe cap 12 and the outer peripheral surface of the tube body 11.

[0133] In addition, a replaceable extraction membrane can be provided in the extraction chamber of the present invention, or an extraction membrane can be pre-placed in the extraction chamber for easy use to avoid contamination of the external environment. An extraction membrane is provided in the extraction chamber, the waste liquid chamber is connected to the inner side of the extraction membrane, and the sample chamber is connected to the outer side of the extraction membrane.

[0134] As mentioned above, a reagent cavity 1201 is provided in the tube cap 12 of the present invention. In other embodiments of the present invention, a cavity may be provided in the tube body 11 to place reagents, or reagents may be placed in both the tube body 11 and the tube cap 12.

[0135] like Figure 16 In some embodiments of the present invention, the tube body has a piston cavity 1110 and a piston rod 114, the piston cavity 1110 is connected to the extraction cavity, and the inner end surface of the piston cavity 1110 is provided with a first puncture structure; the tube cap 12 is suitable for pressing the piston rod 114 so that the reagent in the piston cavity 1110 enters the extraction cavity, wherein the reagent can be placed in the piston cavity 1110, and when the tube cap 12 moves in a direction parallel to the axis of the tube body 11, the piston rod 114 can be pressed by the tube cap 12, so that the reagent in the piston cavity 1110 enters the extraction cavity.

[0136] Among them, the piston cavity 1110 can be configured to place reagents such as a reaction enzyme (such as a lyophilized powder), and the reaction enzyme can be in powder or other forms. In addition, reagents can be set in both the tube cap 12 and the tube body 11, thereby improving the scope of application of the reagent bottle 100 in the present invention. During actual use, the reagent in the tube cap 12 can be added after the reagent in the piston cavity 1110 is added. That is, during the movement of the tube cap 12, when the sleeve 111 pierces all the reagent cavities 1201 in the tube cap 12, the tube cap 12 drives the piston rod 114 to inject the reagent in the piston cavity 1110 into the extraction chamber. Of course, the order of adding the reagents in the tube cap 12 and the reagents in the tube body 11 can be selected according to actual use.

[0137] Alternatively, as Figure 16 A sealing ring 115 is provided between the outer circumference of the piston rod 114 and the inner circumference of the piston chamber 1110. The sealing ring 115 seals the gap between the piston rod 114 and the piston chamber 1110. The sealing ring 115 seals the gap between the piston rod 114 and the piston chamber 1110, thereby preventing the reagent in the piston chamber 1110 from flowing out and preventing objects outside the piston chamber 1110 from entering the piston chamber 1110, thereby improving the stability of the reagent bottle 100.

[0138] The outer circumference of piston rod 114 is provided with a first sealing groove, into which sealing ring 115 is inserted, and sealing ring 115 protrudes from the outer circumference of piston rod 114. This ensures that sealing ring 115 is stably mounted on piston rod 114. Furthermore, the inner circumference of piston chamber 1110 is provided with a second sealing groove, and the portion of sealing ring 115 protruding from the outer circumference of piston rod 114 is adapted to be inserted into second sealing ring 115 and to be adapted to slide out of the second sealing groove. Thus, before piston rod 114 begins to move, the cooperation of the first sealing groove, the second sealing groove, and sealing ring 115 ensures a stable seal between piston rod 114 and the inner circumference of piston chamber 1110. When piston rod 114 is driven to move by tube cap 12, sealing ring 115 can be slid out of the second sealing groove, achieving stable extension and retraction of piston rod 114. Piston rod 114 and piston chamber 1110 are constructed to resemble a syringe, facilitating the injection of reagents into the extraction chamber.

[0139] Of course, in the present invention, the sealing ring 115 can also be fixed on the inner circumferential surface of the sealing cavity, and the piston rod 114 can be moved relative to the sealing ring 115.

[0140] Combine Figure 14 and Figure 16 In some embodiments of the present invention, an inner tube 121 is provided within the tube cap 12. The inner tube 121 extends along the axis of the tube body 11. The tube cap 12 is adapted to drive the inner tube 121 to press against the piston rod 114. This improves the stability of the driving of the piston rod 114. In combination with the aforementioned embodiments, multiple reagent chambers 1201 are provided within the inner tube 121. Therefore, while the inner tube 121 creates multiple reagent chambers 1201 within the tube cap 12, it can also press against and drive the piston rod 114, thereby optimizing and simplifying the structure of the reagent bottle 100.

[0141] Optionally, a plurality of reagent cavities 1201 are provided in the inner tube 121 and are spaced apart along the axis of the tube body 11. The inner tube 121 is suitable for being inserted into the tube body 11 from the second end of the tube body 11, so as to allow the sleeve 111 to pierce and connect the plurality of reagent cavities 1201 in sequence and allow the liquid reagent in the reagent cavity 1201 to enter the sample cavity 1102.

[0142] As mentioned above, combined with Figures 13 to 24The present invention provides a reagent bottle 100, wherein a sleeve 111 and a tube body 11 may be concentrically cylindrical. The hollow structure of the sleeve 111 may constitute the aforementioned sample chamber 1102. A partition structure may be provided within the cavity structure between the sleeve 111 and the tube body 11 to form a piston chamber 1110. A piston rod 114 is provided and telescopically insertable into the piston chamber 1110. The sleeve 111 and the tube body 11 may be integrally formed, and the partition structure constituting the piston chamber 1110 may also be integrally formed with the sleeve 111 and the tube body 11. A second coarse thread 1104 and a fine thread are provided on the outer circumferential surface of the second end of the tube body 11, and the fine thread cooperates with the second coarse thread. The cap 12 is rotatably mounted on the tube body 11, and a first coarse thread 1205 is provided on the inner circumference of the cap 12. The first coarse thread 1205 cooperates with the second coarse thread 1104 to achieve threaded transmission between the cap 12 and the tube body 11, while the fine thread on the outer circumference of the tube body 11 can provide resistance to the rotation of the cap 12. A sealing rib 1204 is provided on the inner end surface of the cap 12. When the tube body 11 is inserted into the top of the cap 12, the circumference of the second end of the tube body 11 can cooperate with the sealing rib 1204 to improve the sealing effect. An inner tube 121 can be provided within the cap 12. The inner tube 121 is shaped like a bell mouth and contains multiple diaphragms 122 arranged at intervals. The multiple diaphragms 122 form multiple reagent chambers 1201 within the inner tube 121, and different reagents can be stored in the multiple reagent chambers 1201. During use, a sample is placed in the sleeve 111, the cap 12 is placed on the tube body 11, and the cap 12 is rotated so that the cap 12 and the center area of ​​the diaphragm 122 face each other. As the cap 12 rotates, the diaphragm 122 contacts the sleeve 111, and the first coarse thread 1205 contacts the fine thread. Further rotation of the cap 12 punctures the diaphragm 122, and the reagent in the reagent chamber 1201 enters the sleeve 111, where it comes into contact and mixes with the sample in the sample chamber 1102. As the cap 12 rotates, multiple reagent chambers 1201 can be punctured through the sleeve 111, thereby completing the delivery of multiple reagents.

[0143] At the same time, when the tube cap 12 moves to a predetermined position, the tube cap 12 will contact the piston rod 114. At this time, the piston rod 114 will provide a reverse force to the tube cap 12, slowing down the movement of the tube cap 12, so as to facilitate the full mixing of the reagent and the sample, and avoid the simultaneous mixing of multiple reagents affecting the detection effect. At the same time, reagents can also be set in the piston chamber 1110. In this way, when the tube cap 12 moves to a specific position, the tube cap 12 can drive the piston rod 114 to inject the reagent in the piston chamber 1110 into the extraction chamber.

[0144] Among them, test paper, extraction membrane 118, etc. can be placed in the extraction chamber of the present invention.

[0145] As previously described, the present invention describes a method for adding reagents to cannula 111. Different reagents can be added to cannula 111 to achieve the desired detection. Furthermore, the reagents added to cannula 111, along with the sample, are ultimately mixed and then introduced into the extraction chamber. The following describes, with reference to the accompanying figures, how reagents enter and exit the extraction chamber in some embodiments of the present invention.

[0146] Combine Figure 14 、 Figure 16 as well as Figures 22 to 24 In some embodiments of the present invention, a waste liquid chamber is constructed between the sleeve 111 and the tube body 11, and the extraction chamber is connected to the waste liquid chamber. A sample chamber 1102 is constructed inside the sleeve 111, and a waste liquid chamber is constructed outside the sleeve 111. During use, reagents can be added to the sample chamber 1102, and the reagents can enter the extraction chamber through the sample chamber 1102. Excess reagents can flow back into the waste liquid chamber through the extraction chamber to improve the stability of the reagent bottle 100. An extraction membrane 118 (for example, for nucleic acid extraction) can be set in the extraction chamber. The extraction membrane 118 can facilitate the retention of reagents and samples in the extraction chamber, while the excess part enters the waste liquid chamber to avoid waste.

[0147] In some embodiments of the present invention, the first end of the tube body 11 is provided with an inner end plate 112 and an outer end plate 113, an extraction chamber is constructed between the inner end plate 112 and the outer end plate 113, and a first through hole 1106 and a second through hole 1113 are constructed on the inner end plate 112. The first through hole 1106 connects the sample chamber 1102 and the extraction chamber, and the second through hole 1113 connects the waste liquid chamber and the extraction chamber. By providing the first through hole 1106 and the second through hole 1113, the sample chamber 1102 and the waste liquid chamber can be easily connected to the extraction chamber, allowing the reagent to circulate within the sample chamber 1102, the extraction chamber, and the waste liquid chamber, completing tasks such as sample extraction and achieving the purpose of sample detection.

[0148] Alternatively, as Figure 22 and Figure 23 A plurality of first through holes 1106 and a plurality of second through holes 1113 are provided on the inner end plate 112. The plurality of first through holes 1106 are arranged at intervals along the circumference of the inner end plate 112, and the plurality of second through holes 1113 are arranged at intervals along the circumference of the inner end plate 112, thereby improving the efficiency of the reagent entering the extraction chamber and contacting the extraction membrane 118 in the extraction chamber, and also facilitating the return of excess reagent to the waste liquid chamber.

[0149] Furthermore, the distance between the first through hole 1106 and the sample chamber 1102 is greater than the distance between the second through hole 1113 and the sample chamber 1102. In this case, after the reagent enters the extraction chamber, it will flow back to the waste liquid chamber after passing through the extraction membrane 118, thereby preventing the reagent from directly flowing back to the waste liquid chamber through the extraction chamber, thereby providing sufficient reaction time for the reagent and sample.

[0150] Combine Figure 16 and Figure 21 In some embodiments of the present invention, an annular plate 116 is provided at the first end of the tube body 11. The inner circumference of the annular plate 116 is connected to the sleeve 111, while the outer circumference of the annular plate 116 is connected to the tube body 11. A plurality of first guide grooves 1119 are provided on the outer end surface of the annular plate 116. Each of the plurality of first guide grooves 1119 is connected to the sample chamber 1102 and extends in a divergent shape away from the sample chamber 1102. The first guide grooves 1119 are connected to the first through-hole 1106. This increases the time and distance that the reagent travels when entering and exiting the extraction chamber, providing more time for the reagent and sample to react, thereby enhancing the extraction effect.

[0151] Optionally, combined Figure 16 and Figure 21 A third through hole 1114 is provided on the annular plate 116. The third through hole 1114 is spaced apart from the first guide groove 1119 and directly faces the second through hole 1113. The second through hole 1113 and the third through hole 1114 cooperate to connect the extraction chamber and the waste liquid chamber. This facilitates the connection between the extraction chamber and the waste liquid chamber, prevents excessive pressure caused by excessive reagent in the extraction chamber, and allows subsequent reagents to quickly enter the extraction chamber.

[0152] Alternatively, as Figure 23 The outer surface of the inner end plate 112 is provided with a receiving groove 1115, which is covered by the outer end plate 113 to form an extraction chamber. The bottom surface of the receiving groove 1115 is provided with crisscrossing flow grooves 1116, and the second through-hole 1113 is connected to the flow grooves 1116. This facilitates the placement of the extraction membrane 118 and the like in the extraction chamber, further increasing the coverage of the reagent in the extraction chamber, and facilitating the extraction of the extraction membrane 118.

[0153] Combine Figure 23 It can be seen that the receiving groove 1115 is in the shape of a circular groove, and a circular flow groove 1116 is provided on the bottom surface of the receiving groove. A plurality of annular ribs are provided in the flow groove 1116. The plurality of annular ribs are arranged at intervals from the inside to the outside, and the plurality of annular ribs are connected by a plurality of radial grooves, thereby facilitating the reagent to flow evenly to the extraction membrane in the extraction chamber.

[0154] like Figure 24 In some embodiments of the present invention, a central groove 1117 and a second guide groove 1118 are provided on the inner side of the outer end plate 113. The central groove 1117 constitutes a part of the extraction chamber. The second guide groove 1118 is connected to the central groove 1117 and extends divergently in a direction away from the central groove 1117. The second guide groove 1118 is connected to the first through hole 1106.

[0155] The reagent bottle 100 according to the embodiment of the present invention has a structure in which the multi-layer diaphragm 122 separates the reagents, and is suitable for detection reactions that require the detection reagents to be added in batches.

[0156] There are many different modes in the specific technical solutions: (1) Figure 14 The multi-layer design shown is to rotate the tube cap 12 and add samples by puncturing step by step; (2) different reagents are sealed in the same plane, and the second puncture structure is designed at the edge position. The first reagent is punctured by the downward displacement of the tube cap 12, and the puncture of other reagent cavities 1201 can be done by rotating the tube cap 12 to cut the reagent separation membrane 122 one by one.

[0157] The reagent bottle 100 of the present invention may be provided with a rotation scale on the bottle body to indicate the type of reagent to be added.

[0158] The present invention stores multi-component reagents in an integrated reaction tube. Reaction reagents are stored in containers, and each component is physically separated by a "splitter" (which can be a film or a thin sheet) to prevent cross-contamination. The position of each reagent component in the tube is set according to experimental needs. The number of reagent components is also set according to experimental needs. The sample can be placed into the sleeve 111 after opening the lid, and the tube cap is then screwed on. During use, the sleeve 111 is displaced by threaded rotation or pressure, puncturing the diaphragms 122 of the different reagent components one by one, allowing the different reagent components to flow into the sleeve 111 in sequence and, after passing through the sample, into the extraction chamber for reaction. Reagent components that do not need to flow through the sample can flow into the extraction chamber through separate microchannels. The extraction chamber is connected to the sample chamber 1102 and the waste liquid chamber via microchannels. Multiple groups of microchannels (multiple first through-holes 1106 and multiple second through-holes 1113) can filter large particles and prevent blockage.

[0159] An extraction membrane 118 can be installed within the extraction chamber. This thin membrane (optimized height 0.2 mm) provides a large heating surface, uniform heat conduction, and shortened PCR reaction time. For particulate samples, the focal plane of the microscopic image is stable. The tube cap 12 is designed with a step corresponding to the liquid level of each reagent component. When the cap reaches this position, resistance increases, serving as a position reminder during manual operation. The outer surface of the tube body 11 can be printed with reagent component position lines to serve as a position reminder during manual operation.

[0160] The reagent bottle 100 of the present invention can be operated manually or on a machine.

[0161] The tube body 11 and cap 12 of the present invention are sealed after the last reagent component flows into the cannula 111. All reagents are sealed within the tube to prevent leakage. The waste liquid chamber can be filled with absorbent material (such as filter paper or sponge). During operation, waste liquid entering the waste liquid chamber is absorbed by the absorbent material, preventing backflow into the microchannel.

[0162] The present invention does not require any auxiliary tools to achieve nucleic acid extraction.

[0163] The PCR reagent bottle 100 of the present invention focuses on the structure of the liquid flow channel, the chamber for dispensing enzymes and eluents, and the PCR reaction chamber. It is suitable for directly adding extracted nucleic acids. The dispensing enzymes in each reagent chamber 1201 of the present invention are freeze-dried. Each reagent chamber 1201 of the present invention can be provided with: sample preservation solution, lysis solution, cleaning solution 1, cleaning solution 2, eluent, and enzyme reaction system. The extraction chamber of the present invention can serve as a PCR reaction chamber, and the reaction solution can enter the extraction chamber from the sample chamber 1102 or enter the waste liquid chamber from the extraction chamber.

[0164] The reaction can be promoted by heating the extraction membrane in the extraction chamber at different temperatures, and the detection can be achieved by observing with different filters.

[0165] In addition, the present invention also provides a detection system, which includes: a positioning module, a heating module, a shooting module and a reagent bottle, wherein the reagent bottle is the reagent bottle according to the aforementioned embodiment, wherein the positioning module has an insertion port, the first end of the reagent bottle is suitable for being inserted into the insertion port and positioned by the positioning module, the heating module is used to heat the extraction chamber, and the shooting module is opposite to the end face of the first end of the reagent bottle so as to be suitable for obtaining a fluorescent quantitative image.

[0166] According to the detection system of an embodiment of the present invention, it is possible to realize the addition of multiple reagents in a closed tube state, and after the actual addition of multiple reagents, reactions and nucleic acid extraction and other work can be carried out in the closed tube body. Moreover, the reagent bottle can also be used as a reaction chamber. In the process of obtaining fluorescent quantitative images, there is no need to remove the extraction membrane, but to directly obtain and react, thereby effectively improving the detection accuracy and avoiding pollution to the external environment and avoiding the influence of the external environment on the detection process.

[0167] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A reagent bottle, characterized in that: The reagent bottle comprises: A tube body, wherein a sleeve is provided in the tube body, a sample chamber is constructed in the sleeve, a waste liquid chamber is constructed between the sleeve and the tube body, and an extraction chamber is provided at the first end of the tube body, wherein the waste liquid chamber and the sample chamber are both connected to the extraction chamber via a microchannel; a tube cap, the tube cap covering the second end of the tube body, The tube body is further provided with a piston chamber and a piston rod, and the piston chamber is connected to the extraction chamber. The inner end surface of the piston rod is provided with a first puncturing structure. The tube cap is movably connected to the tube body so as to be suitable for pushing the piston rod to puncture the reagent contained in the piston chamber for closed-tube extraction. An inner tube is provided in the tube cap, the inner tube extends along the axis of the tube body, and the tube cap is adapted to drive the inner tube to press against the piston rod; An extraction membrane is provided in the extraction cavity, the waste liquid cavity is communicated with the inner side of the extraction membrane, and the sample cavity is communicated with the outer side of the extraction membrane.

2. The reagent bottle according to claim 1, characterized in that A sealing ring is provided between the outer circumferential surface of the piston rod and the inner circumferential surface of the piston cavity.

3. The reagent bottle according to claim 2, characterized in that A first sealing groove is provided on the outer circumferential surface of the piston rod, the sealing ring is embedded in the first sealing groove, and the sealing ring protrudes from the outer circumferential surface of the piston rod.

4. The reagent bottle according to claim 2, characterized in that A second sealing groove is provided on the inner circumference of the piston cavity, and the portion of the sealing ring protruding from the outer circumference of the piston rod is suitable for being embedded in the second sealing groove and suitable for sliding out from the second sealing groove.

5. The reagent bottle according to claim 1, characterized in that The tube body is provided with a second puncturing structure, and the tube cap is provided with a closed reagent cavity. The tube cap is movably connected to the tube body so as to be suitable for the second puncturing structure to puncture the reagent cavity and connect the reagent cavity with the sleeve for closed tube extraction.

6. The reagent bottle according to claim 5, characterized in that A plurality of reagent chambers are provided in the tube cap at intervals, and the plurality of reagent chambers are suitable for being punctured in sequence by the second puncturing structure and connected to the sleeve in sequence.

7. The reagent bottle according to claim 6, characterized in that The tube cap is movably connected to the tube body along the axial direction of the tube body, and the plurality of reagent chambers are spaced apart along the axial direction of the tube cap.

8. The reagent bottle according to claim 6, characterized in that An inner tube is provided in the tube cap, and the inner tube extends along the axis of the tube body. The end of the inner tube away from the sleeve is closed and the end toward the sleeve is open. A plurality of diaphragms are provided in the inner tube, and the plurality of diaphragms are arranged at intervals along the axial direction of the tube body. The reagent chamber is constructed between each adjacent two diaphragms and between the diaphragm and the closed end of the inner tube.

9. The reagent bottle according to claim 6, characterized in that: The tube cap is rotatably connected to the tube body, and a plurality of reagent chambers are arranged at intervals along the circumference of the tube cap.

10. The reagent bottle according to any one of claims 5 to 9, characterized in that: The tube cap is provided with a sample preservation solution, a lysis solution, a first cleaning solution, a second cleaning solution and an air column in the order of the puncture of the second puncture structure.

11. The reagent bottle according to any one of claims 5 to 9, characterized in that: The end of the sleeve is provided with a second puncture structure, and the end of the sleeve is provided with a plurality of notches spaced apart along the circumference of the sleeve.

12. The reagent bottle according to any one of claims 1 to 9, characterized in that: The pipe cap is threadably matched with the pipe body.

13. The reagent bottle according to claim 1, characterized in that A sealing rib is provided on the inner end surface of the tube cap, and the sealing rib is suitable for abutting against the inner circumference of the second end of the tube body, so that the tube cap and the tube body are sealed.

14. A detection system, characterized in that: The detection system includes: a positioning module, a heating module, a shooting module and a reagent bottle, wherein the reagent bottle is a reagent bottle according to any one of claims 1 to 13. Among them, the positioning module has an insertion port, the first end of the reagent bottle is suitable for being inserted into the insertion port and positioned by the positioning module, the heating module is used to heat the extraction chamber, and the shooting module is opposite to the end face of the first end of the reagent bottle to be suitable for obtaining a fluorescent quantitative image.

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