Reagent bottle and detection system

Abstract

The application discloses a reagent bottle and a detection system. The reagent bottle comprises a tube body and a tube cap. A sleeve is arranged in the tube body. A sample cavity is formed in the sleeve. A waste liquid cavity is formed between the sleeve and the tube body. A first end of the tube body is provided with an extraction cavity. The waste liquid cavity and the sample cavity are communicated with the extraction cavity through micro flow channels. The tube body is provided with a first piercing structure. The second end of the tube body is capped by the tube cap. A closed reagent cavity is arranged in the tube cap. The tube cap is movably connected with the tube body. The first piercing structure is adapted to pierce the reagent cavity and make the reagent cavity communicated with the sleeve for closed tube extraction. According to the reagent bottle, the tube body is capped by the tube cap. The addition of the reagent is realized by the movement of the tube cap. The extraction and reaction under the closed tube are completed. The sample is prevented from being polluted or the environment is prevented from being polluted.

Description

TECHNICAL FIELD

[0001] The present application relates to the medical technical field, and in particular to a reagent bottle and a detection system with the same. BACKGROUND

[0002] In the medical diagnosis industry, the detection result of reagent is often needed to diagnose the disease. For the accuracy and stability, the sample is usually collected by a swab and then extracted by reagent. The existing detection device is large in size, and needs to be operated in different negative pressure spaces during the detection process. Moreover, since an open detection mode is adopted, the detection result is easily affected, and the surrounding environment is likely to be polluted. SUMMARY

[0003] An object of the present application is to provide a reagent bottle, which is closed by a cap, and the reagent is added by moving the cap, so that the extraction and reaction under closed tube are completed, and the sample and the environment are prevented from being polluted.

[0004] Another object of the present application is to provide a detection system, which comprises the above-mentioned reagent bottle.

[0005] According to the reagent bottle of the present application, the tube is closed by the cap, and the reagent is added by moving the cap, so that the extraction and reaction under closed tube are completed, and the sample and the environment are prevented from being polluted.

[0006] According to the reagent bottle of the present application, the tube is closed by the cap, and the reagent is added by moving the cap, so that the extraction and reaction under closed tube are completed, and the sample and the environment are prevented from being polluted.

[0007] In addition, the reagent bottle according to the above-mentioned embodiments of the present application can have the following additional technical features.

[0008] Optionally, a plurality of reagent chambers are arranged in the cap at intervals, and the plurality of reagent chambers are adapted to be sequentially punctured by the first puncture structure and sequentially connected to the sleeve.

[0009] Optionally, the cap is movably connected to the tube along the axial direction of the tube, and the plurality of reagent chambers are arranged at intervals along the axial direction of the cap.

[0010] Optionally, an inner tube is arranged in the cap, the inner tube extends along the axis of the tube body, the inner tube is closed at an end away from the sleeve and is open at an end towards the sleeve, a plurality of diaphragms are arranged in the inner tube, the diaphragms are arranged in the axial direction of the tube body, each two adjacent diaphragms and the diaphragm and the closed end of the inner tube form the reagent cavity.

[0011] Optionally, the diaphragm comprises a rigid film layer and a flexible film layer, the rigid film layer is connected to the periphery of the flexible film layer, the rigid film layer and the flexible film layer are connected in a flat plate shape, the flexible film layer is opposite to the sleeve in the axial direction of the tube body, and the thickness of the flexible film layer is less than the thickness of the rigid film layer.

[0012] Optionally, the flexible film layer and the rigid film layer are configured as a recess structure with an opening towards the sleeve.

[0013] Optionally, a plurality of strip-shaped grooves are arranged on the surface of the flexible film layer, the strip-shaped grooves are connected at one place and are configured as a diverging shape.

[0014] Optionally, the inner tube is configured as a variable-diameter tube in the direction away from the sleeve, and a plurality of steps are arranged on the inner circumferential surface of the inner tube, the diaphragm is arranged in the sleeve and supported on the steps on the inner circumferential surface of the inner tube.

[0015] Optionally, an annular clamping groove is arranged on the inner end surface of the cap, an annular clamping hook is arranged on the end of the inner tube, and the annular clamping hook is clamped with the annular clamping groove.

[0016] Optionally, the cap is rotatably connected to the tube body, and a plurality of reagent cavities are arranged in the circumferential direction of the cap.

[0017] Optionally, a sample preservation liquid, a lysis liquid, a first washing liquid, a second washing liquid and an air column are arranged in the cap in the order of piercing of the first piercing structure.

[0018] Optionally, the end of the sleeve is configured as the first piercing structure, and a plurality of notch grooves are arranged in the circumferential direction of the sleeve.

[0019] Optionally, the cap is threadedly connected to the tube body.

[0020] Optionally, the cap is sleeved on the outside of the tube body, a first coarse thread is arranged on the inner circumferential surface of the cap, a second coarse thread is arranged on the outer circumferential surface of the tube body, and the first coarse thread and the second coarse thread are matched.

[0021] Optionally, a fine thread is arranged on the outer circumferential surface of the tube body, the fine thread is matched with the second coarse thread, and the first coarse thread is in contact with the fine thread when the sleeve pierces the reagent cavity.

[0022] Optionally, a sealing rib is arranged on the inner end surface of the cap, the sealing rib is adapted to abut the inner circumferential edge of the second end of the tube body, so that the cap is sealingly matched with the tube body.

[0023] Optionally, an anti-falling groove is arranged on the outer circumferential surface of the first end of the tube body, the anti-falling groove is arranged along the circumferential direction of the tube body.

[0024] Optionally, a plurality of tooth grooves are arranged on the outer circumferential surface of the cap, the tooth grooves are parallel to the axis of the tube body, and the plurality of tooth grooves are arranged along the circumferential direction of the cap.

[0025] Optionally, an anti-skid rib is arranged on the outer circumferential surface of the first end of the tube body, the anti-skid rib extends along a direction parallel to the axis of the tube body, and a plurality of anti-skid ribs are arranged along the circumferential direction of the tube body.

[0026] Optionally, an extraction membrane is arranged in the extraction cavity, the waste liquid cavity is communicated to the inner side of the extraction membrane, and the sample cavity is communicated to the outer side of the extraction membrane.

[0027] The detection system according to the embodiment of the present application comprises a positioning module, a heating module, a shooting module, and a reagent bottle, wherein the reagent bottle is the reagent bottle according to the foregoing, the positioning module has an insertion opening, the first end of the reagent bottle is adapted to be inserted into the insertion opening and positioned by the positioning module, the heating module is used for heating the extraction cavity, and the shooting module is opposite to the end surface of the first end of the reagent bottle to be adapted to acquire a fluorescence quantitative image. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 is a schematic diagram of a detection device according to an embodiment of the present application.

[0029] Figure 2 is a schematic diagram of a detection device according to an embodiment of the present application after removing the shell.

[0030] Figure 3 is a partial schematic diagram of a detection device according to an embodiment of the present application.

[0031] Figure 4 is a partial schematic diagram of a detection device according to an embodiment of the present application in another direction.

[0032] Figure 5 is a schematic diagram of a mounting seat and a positioning assembly of a detection device according to an embodiment of the present application.

[0033] Figure 6 is a schematic diagram of the mounting base and heating element, hot air module of the detection device of one embodiment of the present application.

[0034] Figure 7 is a schematic diagram of the mounting base of the detection device of one embodiment of the present application.

[0035] Figure 8 is a schematic diagram of the detection device of one embodiment of the present application.

[0036] Figure 9 is Figure 8 is a schematic diagram showing the structure after removal of the mounting shell.

[0037] Figure 10 is a schematic diagram of the cooperation of the turntable, probe, filter assembly in the detection device of one embodiment of the present application.

[0038] Figure 11 is a schematic diagram of the cooperation of the turntable, probe, filter assembly and sensing element in the detection device of one embodiment of the present application.

[0039] Figure 12 is a schematic diagram of the inner side of the power connection structure of the detection device of one embodiment of the present application.

[0040] Figure 13 is a schematic diagram of the reagent bottle of one embodiment of the present application.

[0041] Figure 14 is a sectional view of the reagent bottle of one embodiment of the present application.

[0042] Figure 15 is Figure 14 is a partial enlarged schematic diagram of the A area of the middle circle.

[0043] Figure 16 is a sectional view of the reagent bottle of one embodiment of the present application.

[0044] Figure 17 is a schematic diagram of the septum of the reagent bottle of one embodiment of the present application.

[0045] Figure 18 is a schematic diagram of the septum of the reagent bottle of one embodiment of the present application.

[0046] Figure 19 is a schematic diagram of the tube body of the reagent bottle of one embodiment of the present application.

[0047] Figure 20 is a schematic diagram of the tube body of the reagent bottle of one embodiment of the present application.

[0048] Figure 21 is a schematic diagram of the tube body of the reagent bottle of one embodiment of the present application.

[0049] Figure 22 is a schematic view of the inner side of the inner end plate of the reagent bottle according to an embodiment of the present application.

[0050] Figure 23 is a schematic view of the outer side of the inner end plate of the reagent bottle according to an embodiment of the present application.

[0051] Figure 24 is a schematic view of the inner side of the outer end plate of the reagent bottle according to an embodiment of the present application. DETAILED DESCRIPTION

[0052] Embodiments of the present application are described in detail below with reference to the accompanying drawings, wherein the same or like reference numerals in the drawings represent the same or like elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to explain the present application, and should not be understood as limiting the present application.

[0053] The detection device 200 according to an embodiment of the present application comprises a positioning module, and the positioning module is provided with an insertion opening 201 for inserting the reagent bottle 100. After the reagent bottle 100 is inserted, the reagent bottle 100 can be locked, heated, heat-sealed, and subjected to quantitative fluorescence detection, etc. In this embodiment, after the reagent bottle is inserted into the insertion opening, the reagent bottle can be locked by the positioning module. The different operation devices of the reagent bottle 100 are described below with reference to the accompanying drawings.

[0054] In combination with Figures 1 to 7 , the device for heating the reagent bottle 100 according to an embodiment of the present application is described, which comprises a plurality of heating members 22 and a driving device 23. The plurality of heating members 22 are respectively movable to selectively heat the extraction membrane in the reagent bottle 100. That is, the plurality of heating members 22 can all heat the extraction membrane in the reagent bottle 100. In general, in order to facilitate the control of the heating temperature, one or more of the plurality of heating members 22 can be used to heat the reagent bottle 100, so as to avoid damaging the reaction reagent and to avoid fatigue, thereby improving the service life of the detection device 200. The driving device 23 is connected with the heating members 22 to drive the heating members 22 to move.

[0055] The detection device 200 according to an embodiment of the present application can selectively heat the extraction membrane in the reagent bottle 100 by using the plurality of heating members 22, so as to meet different detection temperatures, thereby improving the application range of the detection device 200, and at the same time, the detection under different temperature conditions in the same detection target can be met.

[0056] The heating element 22 in the present application can move in multiple directions to achieve approaching and moving away from the reagent bottle 100, that is, switching between heating and not heating the reagent bottle 100, for example, approaching and moving away from the reagent bottle 100 can be achieved by rotating the heating element 22; it can also be arranged to move radially along the insertion hole to achieve approaching and moving away from the reagent bottle 100, in addition, some embodiments of the present application are also provided to achieve heating of the reagent bottle 100.

[0057] In some embodiments of the present application, the heating element 22 is movably arranged on the positioning module in a direction parallel to the axis of the insertion port 201. That is, the heating element 22 moves in a direction parallel to the axis of the insertion port 201 to approach and move away from the reagent bottle 100, thereby achieving heating and not heating the reagent bottle 100, which can avoid interference between the heating element 22 and other devices (such as the device for rotating the cap of the tube described below, the device for locking the tube body, etc.), improve the stability of the movement of the heating element 22, and simplify the structure of the detection equipment 200, facilitating the design, production and maintenance of the detection equipment 200.

[0058] As Figure 5 The heating element 22 has a heating end for heating the reagent bottle 100, and the heating end is used to approach or abut against the reagent bottle 100 for heating the reagent bottle 100. The heating end is opposite to the insertion port 201 along the axis of the insertion port 201, and the projections of the heating ends of the plurality of heating elements 22 in the direction of the axis of the insertion port 201 are configured to have an annular shape with an observation port. Thus, the reagent bottle 100 can be conveniently heated, and at the same time, by providing the observation port, interference with the viewing of the reagent bottle 100 by the detection device (such as the probe 272 described below) can be avoided, and in the case of facilitating heating, observation can be facilitated to improve detection efficiency.

[0059] As Figure 6 The driving device 23 includes a plurality of driving devices corresponding to the plurality of heating elements 22, and the driving device 23 includes a heating motor and a heating cam. The heating cam is rotatably embedded in the corresponding heating element 22, and the heating motor is connected with the heating cam for driving the corresponding heating element 22 to move. Specifically, the heating element 22 can be provided with a plug-in hole, and the size of the plug-in hole can be larger than the size of the heating cam. Since the heating cam is a non-rotational body shape relative to the motor shaft of the heating motor, the outer periphery of the heating cam has positions with different distances from the motor shaft of the heating motor, thereby achieving driving of the heating element 22. Of course, other structures in the prior art can also be used to achieve driving of the heating element 22 in the present application.

[0060] As Figure 6 and Figure 7The positioning module comprises a mounting base 26, the heating member 22 and the driving device 23 are arranged on the mounting base 26 and away from the insertion opening 201, the mounting base 26 is provided with a matching hole 203, and the heating member 22 is adapted to extend into the matching hole 203 to heat the reagent bottle 100. By arranging the mounting base 26, the integration of the heating device can be facilitated, the assembly and maintenance of the detection equipment 200 are facilitated, and the stability of the movement of the heating member 22 is ensured.

[0061] As shown in Figure 5 , the mounting base 26 is provided with a positioning assembly opposite to the insertion opening 201 and adapted to accommodate and position the end of the reagent bottle 100. In use, the reagent bottle is inserted from the insertion opening 201, and the end of the reagent bottle is inserted into the positioning assembly, and the reagent bottle is positioned by the positioning assembly, so that the pipe body of the reagent bottle is fixed, and the pipe cap is conveniently rotated.

[0062] As shown in Figure 5 , the positioning assembly can comprise a positioning ring 211 adapted to accommodate the end of the reagent bottle 100. In use, the end of the reagent bottle is inserted into the positioning ring 211.

[0063] Optionally, as shown in Figure 5 , the inner circumferential surface of the positioning ring 211 is provided with a positioning rib 212 extending in a direction parallel to the axis of the insertion opening 201, and the positioning rib 212 is used to position the reagent bottle 100 in the circumferential direction of the insertion opening 201. Correspondingly, the bottle body of the reagent bottle 100 is provided with a positioning groove, which can be a structure matched with the positioning rib 212. In the assembly process, the reagent bottle 100 can be inserted into the insertion opening 201, and the positioning rib 212 is inserted into the positioning groove to fix the bottle body of the reagent bottle 100. The positioning rib 212 can be arranged in an asymmetric form relative to the axis of the insertion opening 201, so that the reagent bottle 100 will be inserted into the insertion opening 201 in a specific orientation. For example, when the heat sealing process is performed, by limiting the orientation of the reagent bottle 100, the heat sealing needle can be inserted into the reagent bottle 100 to complete the heat sealing of the corresponding opening in the reagent bottle 100.

[0064] Optionally, as shown in Figure 5 , the positioning assembly further comprises a latch 213 movably connected to the mounting base 26 in the radial direction of the positioning ring 211, and the latch 213 is used to position the reagent bottle 100 in the axial direction of the insertion opening 201. Correspondingly, the end of the reagent bottle 100 can be provided with an anti-extraction groove, and in use, the latch 213 can be inserted into the anti-extraction groove to position the reagent bottle 100 in the axial direction of the insertion opening 201, so as to prevent the reagent bottle 100 from being extracted from the insertion opening 201.

[0065] In the present application, the reagent can be put into the reagent bottle 100 and mixed with the sample and reacted, etc. after the reagent bottle 100 is inserted into the detection device 200, or the reagent bottle 100 can be inserted into the detection device 200, and the actual putting can be realized through the corresponding structure on the detection device 200.

[0066] In combination Figure 3 And Figure 4 In some embodiments of the present application, the positioning module further comprises a rotating barrel 214 and a driving structure 215, the insertion port 201 is configured in the rotating barrel 214, and the rotating barrel 214 is rotatable around the axis of the insertion port 201, the driving structure 215 is connected with the rotating barrel 214 to drive the rotating barrel 214 to rotate, and the rotating barrel 214 is suitable for locking the cap of the reagent bottle 100 to drive the cap to rotate. In use, the reagent bottle 100 is inserted into the positioning module, wherein the reagent bottle 100 comprises a tube and a cap, and the putting of the reagent is realized by the rotation of the cap relative to the tube. Through the above positioning assembly, the tube of the reagent bottle 100 can be fixed, and the cap can be locked and rotated by the rotating barrel, that is, the rotation of the cap relative to the tube can be realized, so as to complete the putting of the reagent. Therefore, by arranging the rotating barrel 214 and the driving structure 215, the automatic putting of the reagent in the reagent bottle 100 can be realized.

[0067] Optionally, the heating member 22 of the present application is a constant temperature module. Through the constant temperature module, the extraction film in the reagent bottle 100 can be heated, so as to heat the extraction film to a predetermined temperature to complete the detection, improve the detection effect, and avoid fatigue caused by too fast heating speed, improve the structural strength and service life.

[0068] In addition, the present application also provides some technical solutions to realize the heat sealing of the opening in the reagent bottle 100. In some embodiments of the present application, the detection device 200 further comprises a heat sealing device 24 and a heat sealing driving 25, the heat sealing device 24 is opposite to the insertion port 201 along the axis direction of the insertion port 201, the heat sealing device 24 is movably arranged in the positioning module along the axial direction of the insertion port 201, and the heat sealing device 24 comprises a heat sealing needle suitable for being inserted into the reagent bottle 100 to perform heat sealing. The heat sealing driving 25 is arranged on the positioning module, and the heat sealing driving 25 is connected with the heat sealing device 24 to drive the heat sealing device 24 to move. After the reagent bottle 100 is inserted into the detection device 200, the heat sealing needle can be inserted into the reagent bottle 100 by moving the heat sealing device 24, so as to complete the heat sealing of the corresponding opening in the reagent bottle 100, and improve the accuracy of the detection result.

[0069] Optionally, as Figure 6The heat-sealing drive 25 comprises a heat-sealing motor and a heat-sealing cam, the heat-sealing cam is rotatably embedded in the heat-sealing device 24, and the heat-sealing motor is connected with the heat-sealing cam and used to drive the heat-sealing device 24 to move. The driving mode of the heat-sealing drive 25 is basically the same as that of the heating drive.

[0070] Optionally, in combination with the foregoing description, the positioning module comprises a mounting base 26, the heat-sealing device 24 is arranged on the mounting base 26 and faces away from the insertion opening 201, the mounting base 26 is provided with a matching hole 203, and the heat-sealing device 24 is adapted to extend into the insertion opening 201 through the matching hole 203. In this way, the integration of various components in the detection equipment 200 is facilitated, and the assembly and maintenance are facilitated. In combination with the foregoing embodiments, the heat-sealing device 24 and the heating element 22 are arranged on the mounting base 26.

[0071] As shown in FIG. 1, the detection equipment 200 comprises a positioning module, a heating drive, a heat-sealing drive, a detection device and a camera. Figure 5 In some embodiments of the present application, a limiting plate 261 is arranged in the matching hole 203, the limiting plate 261 is a hollow ring, and the limiting plate 261 is provided with a guide hole 262 corresponding to the heat-sealing needle. In this way, the heat-sealing needle is guided, and the stability of the heat-sealing effect is improved. In addition, a guide cylinder can also be arranged on the limiting plate 261, the guide cylinder is a hollow cylindrical structure, the heat-sealing cover can pass through the guide cylinder, and the heat-sealing needle is guided through the guide cylinder. In addition, the guide cylinder is embedded in the heating element 22, so that the heating element 22 is guided through the guide cylinder, and the heating cylinder and the heat-sealing needle are guided through the guide cylinder at the same time, thereby simplifying and optimizing the structure.

[0072] In addition, the detection equipment 200 of the present application further comprises a device for observing the reagent bottle 100. Through the observation of the reagent bottle 100 under different temperature conditions, a reasonable and accurate conclusion can be finally given.

[0073] As shown in FIG. 1, the detection equipment 200 comprises a positioning module, a heating drive, a heat-sealing drive, a detection device and a camera. Figures 8 to 12 In some embodiments of the present application, the camera module comprises a turntable 271, a probe 272, a plurality of filter assemblies 273 and a sensing element 274. The turntable 271 is rotatably connected with the positioning module, the probe 272 is fixed relative to the positioning module and opposite to the end of the insertion opening 201, the plurality of filter assemblies 273 are arranged at intervals around the rotation axis of the turntable 271, the plurality of filter assemblies 273 are driven by the turntable 271 to be opposite to the probe 272 selectively, and the sensing element 274 is configured to cooperate with the probe 272 to take light after passing through the filter assembly 273. In the use process, different filter assemblies 273 can be used to observe the reagent bottle 100 through the rotation of the turntable 271, so as to meet the detection requirements and improve the accuracy of the detection results.

[0074] The sensing element 274 can be arranged on each filter assembly 273 to improve detection efficiency, or one sensing element 274 can be arranged to observe the form effect under different filter assemblies 273. Figure 10 and Figure 11 The sensing element 274 is fixed relative to the positioning module and opposite to the probe 272, and the plurality of filter assemblies 273 are driven by the turntable 271 to be opposite to the probe 272 and the sensing element 274. By fixing the sensing element 274 to the positioning module, the wiring of the sensing element 274 can be simplified, the wire entanglement caused by the rotation of the turntable 271 can be avoided, the stability of signal transmission can be effectively improved, and the number of sensing elements 274 can be reduced to reduce the cost.

[0075] In combination with Figure 11 and Figure 12 In some embodiments of the present application, the turntable 271 is provided with a plurality of electrically connected structures insulated from each other, the electrically connected structure includes an insulating column 281, an electrically connected ring 282 and a conductive part 283, the insulating column 281 is fixed relative to the positioning module, the electrically connected ring 282 is rotatably sleeved on the insulating column 281, the conductive part 283 is arranged on the insulating column 281 and slidably abuts against the electrically connected ring 282, and the conductive part 283 is electrically connected with the electrically connected ring 282; wherein the filter assembly 273 is electrically connected with the electrically connected ring 282. The conductive part 283 can be connected to the power supply through a wire harness. Since the insulating column 281 is fixedly connected with the positioning module, the electrical connection between the conductive part 283 and the corresponding structure on the positioning module is positioned, and the wire winding problem does not occur. The electrically connected ring 282 can be fixedly connected with the turntable 271. Since the conductive part 283 abuts against the electrically connected ring 282, stable electrical connection can be achieved during relative movement, so that stable electrical connection of the conductive part 283 can be achieved, and then the filter assembly 273 can be connected to the power supply.

[0076] In addition, an insulating ring 284 can be arranged between the conductive part 283 and the turntable 271 to electrically isolate the conductive part 283 from the turntable 271, thereby improving the safety of the electrically connected structure.

[0077] The insulating column 281 is a hollow structure and has a through hole on the peripheral surface, and the conductive part 283 is a ball plunger arranged in the insulating column 281 and extending out of the through hole, and the ball head of the ball plunger abuts against the conductive part 283. Thus, the stability of the conductive structure can be improved.

[0078] Optionally, as Figure 11 The turntable 271 includes a hub 2711 and a plurality of filter seats 2712, the plurality of filter seats 2712 are connected with the hub 2711 and arranged around the hub 2711, and the plurality of filter assemblies 273 are matched with the plurality of filter seats 2712 one by one.

[0079] In addition, opposite ends of the wheel hub 2711 are provided with mounting grooves, and the mounting grooves are provided with electrical connection structures.

[0080] As Figure 8 In some embodiments of the present application, the detection device further comprises a support seat 208 and a mounting shell 209, the mounting shell 209 covers the support seat 208, wherein the inductive element 274 is arranged on the support seat 208, the probe 272 is arranged on the mounting shell 209, and the rotating disc 271 and the plurality of filter assemblies 273 are arranged in the mounting shell 209. Thus, stable installation of various elements can be achieved, and the structure and stability are optimized.

[0081] In addition, the insulating columns 281 of the two electrical connection structures are respectively connected with the support seat 208 and the mounting shell 209. Thus, stable connection of the insulating columns 281 and the positioning module is achieved.

[0082] In addition, a circular cavity can be arranged in the mounting shell 209, and the rotating disc 271 is rotatably arranged in the circular cavity. The rotating disc 271 can be positioned by the circular cavity, and stable rotation of the rotating disc 271 is guided.

[0083] Optionally, the support seat 208 is provided with a rotating shaft, the rotating disc 271 comprises a rotating disc seat and a rotating disc cover, the rotating disc seat is rotatably connected with the rotating shaft, the rotating disc seat is provided with a plurality of mounting grooves arranged around the rotating shaft and used for mounting the filter assemblies 273, and the rotating disc cover covers the rotating disc seat and the mounting grooves. By arranging the rotating disc seat and the rotating disc cover, damage of the filter assemblies 273 by other components can be avoided, the influence of the surrounding environment on the observation result is effectively excluded, and the precision of the observation result is improved.

[0084] Optionally, the rotating disc cover comprises a bottom plate and a plurality of partition plates, the bottom plate covers the rotating disc seat, the bottom plate is provided with holes corresponding to the plurality of filter assemblies 273, the partition plates are connected with the bottom plate and extend towards the rotating disc seat, and the plurality of partition plates are arranged along the circumference of the bottom plate and are spaced apart from each other, and the partition plates are arranged between every adjacent two filter assemblies 273. By the bottom plate, the plurality of filter assemblies 273 can be packaged, and by the partition plates, the plurality of filter assemblies 273 can be conveniently separated, the entry of dust and other impurities into the filter assemblies 273 is reduced, and the precision of the detection result is improved.

[0085] As Figure 11 In some embodiments of the present application, the rotating disc 271 further comprises an outer gear ring 2713, the outer gear ring 2713 surrounds and is fixedly connected with the rotating disc seat, a rotating disc motor is arranged on the support seat or the mounting shell, the rotating disc is driven by the rotating disc motor, and the rotating disc motor is in transmission connection with the outer gear ring 2713. Thus, the rotating disc 271 can be conveniently driven to rotate, and the stability of the rotating process of the rotating disc 271 is improved.

[0086] In combination Figures 1 to 12In combination with the foregoing embodiments, the positioning module further comprises a mounting seat 26, the mounting seat 26 is provided with a heating device for heating the reagent bottle 100, the mounting seat 26 is arranged between the rotating disc 271 and the insertion port 201, the mounting seat 26 is provided with a matching hole 203, the insertion port 201, the matching hole 203 and the probe 272 are opposite along the axis of the insertion port 201. The heating device can heat the extraction membrane, so that a good detection result is obtained, and meanwhile, the mounting seat 26 is arranged between the rotating disc 271 and the insertion port 201, so that the structure of the heating device and the observation device can be simplified, and the matching hole 203 arranged on the mounting seat 26 can facilitate the light to pass through, so that the observation is facilitated and the detection efficiency is improved.

[0087] In the present application, the mounting seat 26 and the supporting seat 208 can be assembled together after being assembled respectively, so that the assembly process is simplified.

[0088] As Figure 1 In some embodiments of the present application, the positioning module comprises a cabinet 21, the insertion port 201 is exposed from the outer surface of the cabinet 21, and the cabinet 21 is provided with a heat dissipation port in communication with the internal space of the cabinet 21. By arranging the heat dissipation port, the heat dissipation of the components in the positioning module can be facilitated, and the working environment of each component in the detection equipment 200 is optimized.

[0089] In the present application, the positioning module, the heating module and the shooting module are arranged in the cabinet.

[0090] Optionally, the detection equipment 200 further comprises a display screen 29, and the display surface of the display screen 29 is configured as part of the appearance surface of the positioning module.

[0091] In addition, the detection equipment further comprises a signal processing module, the signal processing module is arranged on the positioning module and is in signal transmission with the sensing element 274; the display screen 29 is in signal transmission with the signal processing module. By integrating the display screen 29 on the cabinet 21, the miniaturization and portability of the detection equipment 200 can be facilitated.

[0092] As can be seen from the foregoing description, the detection equipment 200 in the present application can be used for PCR detection, and the detection efficiency of PCR can be improved, and the portability of the detection equipment 200 is improved, wherein the detection process can be divided into the following steps:

[0093] ①The mixing of the sample and the reagent in the reagent bottle 100 is completed, that is, the reagent is added to the cavity where the sample is located, and is extracted and collected through the extraction membrane. This process can be completed before the reagent bottle 100 is inserted into the detection equipment 200, or the reagent bottle 100 can be inserted into the detection equipment 200, and the detection equipment 200 automatically completes the process of adding the reagent, wherein the process of adding the reagent and the extraction of the extraction membrane will be described in detail below;

[0094] ②After the reagent bottle 100 is inserted into the insertion port 201, the reagent bottle 100 is locked and positioned by the latch 213, the positioning rib 212 and the like, so that the reagent bottle 100 can be inserted into the detection device 200 according to a predetermined track and direction.

[0095] ③After the reagent bottle 100 is inserted and the reagent dispensing is completed, the reagent bottle 100 can be heat-sealed to seal the extraction cavity in the reagent bottle 100, and the multi-component actual storage reaction is integrally performed in the extraction cavity, and the extraction cavity is separated from the sample cavity and the waste liquid cavity.

[0096] ④Subsequently, the extraction membrane in the reagent bottle 100 can be heated by the heating element 22. Since the extraction membrane and the outer end plate are relatively thin, the whole extraction membrane can be heated by heating one part of the extraction membrane. Three heating elements 22 (of course, one, two or four or more heating elements 22) can be provided to heat the reagent bottle 100. The heating element 22 can be a constant temperature module. The heating element 22 can be raised and lowered along the axis direction of the insertion port 201 to heat and not heat the reagent bottle 100. The plurality of heating elements 22 are uniformly spaced around the insertion port 201, for example, the Sagen heating element 22 is arranged at an interval of 120°. The program can realize that the plurality of heating elements 22 heat the extraction membrane in the reagent bottle 100 respectively.

[0097] ⑤After heating is completed or during heating, the reagent bottle 100 can be observed by the probe 272, the filter assembly 273 and the sensing element 274. The filter assembly 273 can be switched by rotating the rotating disc 271. The filter assembly 273 can be switched by continuous single-direction rotation, which shortens the wavelength switching time. The power supply can be realized by a slip ring type power supply structure. Five different color fluorescent filter assemblies 273 can be provided to improve detection accuracy.

[0098] Through the above steps, the detection of the reagent can be realized, and the detection efficiency is improved. In the present application, the microscopic light path is miniaturized, a high-sensitivity color camera is used, and a colorless fluorescent filter assembly 273 is used to shoot the fluorescent image of each fluorescent channel.

[0099] In combination with the foregoing detection device 200, the present application also provides a reagent bottle 100 which can be applied to the foregoing detection device 200.

[0100] As Figure 13As shown, the present application also provides a reagent bottle 100, which can be applied to the foregoing detection device, and can also be produced, sold and used independently. The reagent bottle 100 comprises a tube body 11 and a tube cap 12, the tube body 11 is provided with an extraction cavity, a sample cavity and a waste liquid cavity, and the sample cavity and the waste liquid cavity are communicated with the extraction cavity through micro flow channels. The extraction cavity is used for reaction and extraction, the sample cavity is used for sample injection, and the waste liquid cavity is used for waste liquid collection. The tube cap 12 is suitable for closing the tube body 11. At least one of the tube cap and the tube body is provided with a closed reagent cavity, and the tube cap is configured to be movable when closing the tube body to be suitable for piercing the reagent cavity, and the reagent cavity is configured to be suitable for reagent flowing into the sample cavity and / or the extraction cavity after being pierced. Therefore, in the reagent cavity of the present application, after the tube cap closes the tube body, the injection of the reagent can still be carried out by moving the tube cap, so as to realize the closed tube injection of the reagent, the closed tube extraction and the closed tube reaction. For example, in the PCR detection, the extraction and reaction of the sample are carried out in the detection tube, so as to effectively avoid the influence of the external environment on the detection process, and also can avoid the influence of the aerosol generated in the detection process on the surrounding environment.

[0101] In addition, in combination with the foregoing embodiments, after the reagent bottle 100 is inserted into the insertion port, the extraction cavity of the reagent bottle 100 can be opposite to the heating module and the shooting module, so as to realize the heating and shooting of the extraction film of the reagent bottle 100, thereby realizing effective quantitative fluorescence detection, and the process of the fluorescence quantitative detection can be completed by one device, and the detection is simplified.

[0102] In some embodiments of the present application, the tube body 11 is provided with a sleeve 111, the first end (referring to the lower end of the tube body 11 in the drawings) of the tube body 11 is provided with an extraction cavity, the sleeve 111 is configured with a sample cavity 1102, and the sleeve 111 and the tube body 11 are configured with a waste liquid cavity. The waste liquid cavity and the sample cavity are communicated with the extraction cavity through micro flow channels. The tube cap 12 covers the second end (referring to the upper end of the tube body 11 in the drawings) of the tube body 11. The sample cavity 1102 can be placed with a sample, for example, a sample swab A collected is placed into the sample cavity 1102, wherein in order to reduce the size of the reagent bottle 100, the sample swab A such as a cotton swab can be broken, and the effective part is placed in the sample cavity 1102. The tube cap 12 covers the tube body 11 and can move along the tube body 11, so that the injection of the reagent can be realized by moving the tube cap 12 along the tube body 11. Different embodiments are provided in the present application to realize the injection of the reagent, for example, the reagent is placed in the tube cap 12 and pierced by the sleeve 111; the reagent can also be placed in the tube body 11, and the injection of the reagent is realized by moving the tube cap 12. During the injection of the reagent, the tube cap 12 closes the tube body 11, which can avoid the generation of aerosol and other pollutants, avoid the pollution of the sample and the reagent in the reagent bottle, and also can avoid the pollution to the external environment. The present application will be described in detail.

[0103] The closed reagent adding mode is adopted in the application, which can prevent bottle pollution and prevent pollution to the external environment, and reduce the influence of the external environment on the extraction of the reagent bottle and the reaction process. Thus, moderate or room temperature storage and work can be realized.

[0104] As Figure 14 In some embodiments of the application, a closed reagent cavity 1201 is arranged in the cap 12, the cap 12 is movably connected to the tube body 11, and the tube body further has a first piercing structure adapted to pierce the reagent cavity 1201 during movement of the cap 12 relative to the tube body 11, and to communicate the reagent cavity 1201 with the sleeve, so that the reagent can still be put, the sample can still be extracted, and the like when the cap 12 closes the tube body 11, and closed tube extraction can be realized. That is, during movement of the cap 12, the first piercing structure in the tube body also moves relative to the cap 12 and pierces the reagent cavity 1201 in the cap 12. When the reagent cavity 1201 in the cap 12 is pierced, the reagent cavity 1201 can be communicated with the sample cavity 1102, so that the reagent in the reagent cavity 1201 is put into the sample cavity 1102. The reagent in the reagent cavity 1201 can be a liquid reagent to facilitate the reagent to be put.

[0105] In the application, the reagent cavity 1201 in the cap 12 can be constructed in various ways, for example, an air bag is arranged in the cap 12, a plurality of partitions are arranged in the cap 12 to construct a plurality of reagent cavities 1201, of course, in order to facilitate the sleeve 111 to pierce and communicate the reagent cavity 1201, an inner tube 121 can also be arranged in the cap 12 to construct the reagent cavity 1201, which will be described in detail below.

[0106] In some embodiments of the application, only one reagent cavity can be arranged in the cap, or a plurality of reagent cavities can be arranged in the cap, and the plurality of reagent cavities are adapted to be pierced by the first piercing structure in turn and connected to the sleeve in turn. When one reagent cavity is arranged in the cap, the reagent can be quickly put after the reagent cavity is pierced, and when a plurality of reagent cavities are arranged in the cap, the actual separate and sequential putting of the plurality of reagent cavities can be realized.

[0107] The plurality of reagent cavities in the cap can be arranged in various ways to facilitate the first piercing structure to pierce.

[0108] Alternatively, the cap is movably connected to the tube body along the axial direction of the tube body, and the plurality of reagent cavities are arranged in the cap in the axial direction. At this time, by moving the cap along the axial direction of the tube body, the plurality of reagent cavities can be pierced in turn, and the plurality of reagent cavities can be sequentially communicated with the sample cavity, and the reagent can be put.

[0109] As Figures 14 to 16The inner tube 121 is inserted into the tube body 11 from the second end of the tube body 11, and the sleeve 111 can sequentially pierce and connect the plurality of reagent cavities 1201. By arranging the plurality of reagent cavities 1201 in the inner tube 121, the reagents in the reagent cavities 1201 can be conveniently delivered. The plurality of reagent cavities 1201 are arranged along the axis of the tube body 11, so that the sleeve 111 can sequentially pierce the plurality of reagent cavities 1201 during the movement of the cap 12, thereby achieving separate delivery of the reagents in the reagent cavities 1201 and meeting different detection requirements.

[0110] Optionally, in combination with Figures 14 to 16 The end of the inner tube 121 away from the sleeve 111 is closed, and the inner tube 121 is provided with a plurality of diaphragms 122 arranged along the axis of the tube body 11. Each adjacent two diaphragms 122 and the closed end of the inner tube 121 form a reagent cavity 1201. By arranging the diaphragm 122, the piercing of the sleeve 111 is facilitated, and the sleeve 111 is further facilitated to pierce and connect the reagent cavities 1201, thereby improving the delivery efficiency of the reagents.

[0111] Further, as Figure 17 and Figure 18 The diaphragm 122 includes a hard film layer 122a and a flexible film layer 122b. The hard film layer 122a is connected to the periphery of the flexible film layer 122b, and the hard film layer 122a and the flexible film layer 122b are connected into a flat plate. The flexible film layer 122b is opposite to the sleeve 111 in the axial direction of the tube body 11, and the thickness of the flexible film layer 122b is lower than the thickness of the hard film layer 122a. In use, the flexible film layer 122b with smaller thickness is opposite to the sleeve 111, so that the sleeve 111 will easily pierce the flexible film layer 122b during the movement of the cap 12, thereby further improving the delivery efficiency of the reagents.

[0112] Optionally, the flexible film layer 122b and the hard film layer 122a are configured as a recess structure with an opening facing the sleeve 111. In use, the end of the sleeve 111 will be inserted into the recess structure, and the sleeve 111 can be guided through the recess structure to facilitate the sleeve 111 to quickly and stably pierce the flexible film layer 122b. Moreover, the recess structure can also play a certain sealing role to enable the reagents in the reagent cavities 1201 to be stably delivered into the sample cavity 1102.

[0113] Optionally, in combination with Figure 17 and Figure 18The surface of the flexible film layer 122b is provided with a plurality of strip-shaped grooves 122c which are connected together and configured in a diverging shape. By providing the strip-shaped grooves 122c, the flexible film layer 122b is more easily punctured, and the efficiency of reagent delivery is improved. Figure 17 and Figure 18 As can be seen, at least one side surface of the flexible film layer 122b is provided with the strip-shaped grooves 122c, and the outer surface of the outermost diaphragm 122 of the inner tube is provided with a plurality of annular ribs which extend in a plurality of concentric circles centered on the sleeve.

[0114] Optionally, in combination with Figures 14 to 16 The inner tube 121 is configured as a variable-diameter tube which gradually decreases in radial dimension in a direction away from the sleeve 111, and a plurality of steps are configured on the inner circumferential surface of the inner tube 121. The diaphragm 122 is arranged in the sleeve 111 and supported on the steps on the inner circumferential surface of the inner tube 121. In other words, the inner tube 121 is configured to include a plurality of tube segments which are sequentially connected in the axial direction of the sleeve 111, and the radial dimension of the tube segment away from the sleeve 111 is smaller than the radial dimension of the tube segment close to the sleeve 111, so that a step facing the sleeve 111 is configured between the adjacent two tube segments, and the diaphragm 122 can be supported on the step. In the process of puncturing the diaphragm 122 through the sleeve 111, the step can provide support for the diaphragm 122, so that the sleeve 111 can stably puncture the diaphragm 122, thereby improving the stability of the puncturing process.

[0115] The inner tube 121 in the present application can be integrally formed with the tube cap 12, and the inner tube 121 and the tube cap 12 can also be provided as separate parts. The integral forming can effectively improve the structural strength of the tube cap 12 and also simplify the forming process, but the integral forming has higher requirements for the mold and higher costs for the mold in the early stage. Therefore, the present application provides a structure in which the tube cap 12 and the inner tube 121 are assembled together after separate forming, so as to simplify the production of the tube cap 12 and reduce costs. Specifically, Figure 14 and Figure 15 The inner end surface of the tube cap 12 is provided with an annular clamping groove, and the end portion of the inner tube 121 is provided with an annular clamping hook 1202 which is clamped with the annular clamping groove. Specifically, a rib structure can be provided on the inner end surface of the tube cap 12 to configure the annular clamping groove. The annular clamping groove can be configured in a form which gradually contracts inward in a direction away from the inner end surface of the tube cap 12, or the radial dimension of the annular clamping groove gradually decreases in a direction away from the inner end surface of the tube cap 12. A structure corresponding to the annular clamping groove can be provided on the inner shell, for example, the annular clamping hook 1202 on the inner shell is provided in a gradually outward expanding shape. Through the cooperation of the annular clamping groove and the annular clamping hook 1202, stable connection between the inner tube 121 and the tube cap 12 can be achieved, and the connection strength between the inner tube 121 and the tube cap 12 is improved.

[0116] In addition, the plurality of reagent cavities can also be arranged at intervals in the circumferential direction of the cap, and the first piercing structure is used to sequentially pierce the plurality of reagent cavities by rotating the cap relative to the tube body.

[0117] As described above, the plurality of reagent cavities can be arranged in the cap, and the plurality of reagent cavities can respectively hold a sample preservation liquid, a lysis liquid, a first washing liquid, a second washing liquid, and an air column, which are sequentially arranged to achieve separate addition. The sample preservation liquid, the lysis liquid, the first washing liquid, the second washing liquid, and the air column are sequentially arranged in the cap along the piercing order of the first piercing structure. For example, in the embodiment in which the plurality of reagent cavities are arranged at intervals along the axial direction of the tube body, the sample preservation liquid, the lysis liquid, the first washing liquid, the second washing liquid, and the air column can be sequentially arranged in the plurality of reagent cavities arranged along the axial direction of the tube body, and the reagent cavity for storing the sample preservation liquid is arranged close to the opening of the cap to facilitate the first addition of the sample preservation liquid into the sample cavity.

[0118] In the present application, the end of the sleeve can be configured as the first piercing structure described above, thereby simplifying the tube body.

[0119] In combination with Figure 14 and Figure 21 In order to further facilitate the piercing of the reagent cavity 1201 by the sleeve 111, a plurality of notched grooves 1103 arranged at intervals in the circumferential direction of the sleeve 111 can be arranged at the end of the sleeve 111. In this way, during the piercing of the reagent cavity 1201 by the sleeve 111, the side edges of the notched grooves 1103 can provide a plurality of piercing points for the sleeve 111 to improve the efficiency of piercing. In addition, the cap 12 in the present application can be arranged in the form of a threaded connection with the tube body 11, and the piercing of the reagent cavity 1201 by the sleeve 111 is achieved by rotating the cap. During this process, the rotation of the cap 12 causes the sleeve 111 to pierce the reagent cavity 1201 in rotation, and by arranging the notched grooves 1103, the force applied by the circumferential edges of the notched grooves 1103 to the reagent cavity 1201 will have a good stress concentration phenomenon to achieve rapid piercing of the reagent cavity 1201.

[0120] As Figure 14In some embodiments of the present application, a sealing rib 1204 is arranged on the inner end face of the cap 12, and the sealing rib 1204 is adapted to abut against the inner periphery of the second end of the tube body 11, so as to seal the cap 12 and the tube body 11. By arranging the sealing rib 1204, when the cap 12 is moved to a predetermined position along the axial direction of the tube body 11, for example, the end of the tube body 11 abuts against the inner end face of the cap 12, the sealing rib 1204 will be in contact with the inner periphery of the tube body 11, so as to seal the cap 12 and the tube body 11, avoid the reagent in the reagent bottle 100 from leaking out, improve the accuracy of the detection result, and avoid waste.

[0121] In the present application, the cap 12 can be moved along the axial direction of the tube body 11 in a straight line, of course, other ways can also be used to realize the movement of the cap 12 relative to the tube body 11. Preferably, in an embodiment of the present application, the cap 12 is threadedly connected with the tube body 11, which not only facilitates the stable connection of the cap 12 and the tube body 11, but also realizes the movement of the cap 12 relative to the tube body 11 through threaded transmission. In addition, threaded transmission can enable the sleeve 111 to have greater force to pierce the reagent cavity 1201, improve the stability of the structure and the efficiency of reagent feeding. Figures 14 to 16 In some embodiments of the present application, the cap 12 is sleeved on the tube body 11, the inner periphery of the cap 12 is provided with a first coarse thread 1205, the outer periphery of the tube body 11 is provided with a second coarse thread 1104, and the first coarse thread 1205 and the second coarse thread 1104 are matched. Through the cooperation of the first coarse thread 1205 and the second coarse thread 1104, the stable connection of the cap 12 and the tube body 11 can be realized.

[0122] The outer periphery of the tube body 11 is also provided with a fine thread, the fine thread is matched with the second coarse thread 1104, and the first coarse thread 1205 is in contact with the fine thread when the sleeve 111 pierces the reagent cavity 1201. Through the cooperation of the first coarse thread 1205 and the second coarse thread 1104, the threaded cooperation of the cap 12 and the tube body 11 can be realized, and the fine thread arranged on the tube body 11 can enhance the resistance to rotating the cap 12, so as to give appropriate feedback to the user and remind the user. The position of the fine thread in the present application can increase the resistance of the cap 12 during the process of piercing the reagent cavity 1201 by the sleeve 111, so as to facilitate the user.

[0123] In addition, as described above, the cap 12 in the present application is provided with a plurality of reagent cavities 1201, and the fine thread can facilitate the reminding of the user. If the required reagent types are less than the actual number of reagent cavities 1201, the user can find and control in time.

[0124] In other words, during the rotation of the cap 12 in this invention, when the wall of the reagent chamber 1201 inside the cap 12 (as described above, the diaphragm 122) begins or is about to begin touching the sleeve 111, the first coarse thread 1205 will engage with the second coarse thread 1104 and the fine thread, thereby providing timely feedback to the user and making it easier for the user to understand the working status of the reagent bottle 100 so as to make adjustments.

[0125] Optionally, combined Figure 16 , Figures 22-24 The first end of the tube body 11 is provided with an inner end plate 112 and an outer end plate 113, forming an extraction cavity between the inner end plate 112 and the outer end plate 113. A first through hole 1106 is formed on the inner end plate 112, which connects the sample cavity 1102 and the extraction cavity. By setting the inner end plate 112 and the outer end plate 113, the first end of the tube body 11 can be easily sealed, and the structure of the extraction cavity is simplified, making it easier to form the tube body 11. In addition, an extraction membrane 118 can be placed in the extraction cavity, and the extraction membrane 118 can be held by the inner end cap and the outer end cap, improving the structural strength and stability of the reagent bottle 100.

[0126] The first and second through holes are suitable for heat sealing by a heat sealing device. Specifically, after the reagent bottle is inserted into the insertion port, a heat sealing needle can be inserted through the outer end plate into the inner end plate to seal the first and second through holes on the inner end plate.

[0127] Furthermore, to facilitate heating and observation of the reagent bottle, the positioning module in the aforementioned embodiments can be used to lock the reagent bottle. The positioning module can lock the body and cap of the reagent bottle, and can also drive the cap to move relative to the body to facilitate reagent dispensing. For example, the positioning module can drive the cap to rotate relative to the body.

[0128] Optionally, combined Figure 13 and Figure 19 An anti-detachment groove 1107 is provided on the outer circumferential surface of the first end of the tube body 11, and the anti-detachment groove 1107 is arranged along the circumference of the tube body 11. The anti-detachment groove 1107 can be an annular shape extending along the circumference of the tube body 11, or it can be a groove extending intermittently along the circumference of the tube body 11. When the tube body 11 is placed in the testing equipment, the tube body 11 can be axially positioned by the corresponding structure on the testing equipment to prevent the reagent bottle 100 from falling out of the testing equipment.

[0129] In conjunction with the preceding embodiments, the positioning module is adapted to cooperate with the anti-dislodgement groove to position the reagent bottle axially along the insertion port, wherein the pin in the aforementioned embodiments can be inserted into the anti-dislodgement groove to lock the reagent bottle.

[0130] In addition, a anti-coming-off protrusion or the like can be arranged on the outer circumferential surface of the first end of the pipe body 11, and axial positioning of the reagent bottle 100 can also be achieved by the anti-coming-off protrusion or the like.

[0131] Optionally, in combination with Figure 19 , a positioning groove 1108 can also be arranged on the outer circumferential surface of the first end of the pipe body 11, and the positioning groove 1108 can extend in a direction parallel to the axis of the pipe body 11. In use, a positioning rib can be arranged at a corresponding position of the detection equipment to achieve circumferential positioning of the pipe body 11.

[0132] The circumferential positioning of the pipe body can be achieved by the aforementioned positioning module, for example, the aforementioned positioning rib is embedded into the positioning groove to achieve circumferential locking of the pipe body.

[0133] Optionally, as Figure 13 , a plurality of tooth grooves 1206 are arranged on the outer circumferential surface of the cap 12, the tooth grooves 1206 are parallel to the axis of the pipe body 11, and the plurality of tooth grooves 1206 are arranged at intervals in the circumferential direction of the cap 12. The plurality of tooth grooves 1206 can increase the friction of the outer circumferential surface of the cap 12, and when the cap 12 is manually or automatically driven to move along the axis of the pipe body 11, the stability of the reagent bottle 100 can be improved.

[0134] The tooth grooves 1206 can be used to improve the stability of manual or motorized rotation of the cap 12, for example, the circumferential locking of the cap can be achieved by the positioning module cooperating with the tooth grooves, and then the reagent can be put by rotating the cap.

[0135] Among them, the tooth grooves 1206 on the outer circumferential surface of the cap 12 can be arranged to extend in a direction parallel to the axis of the pipe body 11.

[0136] Optionally, as Figure 13 and Figure 19 , an anti-skid rib 1109 is arranged on the outer circumferential surface of the first end of the pipe body 11, the anti-skid rib 1109 extends in a direction parallel to the axis of the pipe body 11, and a plurality of anti-skid ribs 1109 are arranged at intervals in the circumferential direction of the pipe body 11. The anti-skid rib 1109 can increase the friction of the pipe body 11, and in use, when the cap 12 is rotated, force needs to be applied to the cap 12 and the pipe body 11 at the same time. Therefore, by arranging the tooth grooves 1206 on the cap 12 and the anti-skid ribs 1109 on the pipe body 11, the rotation of the cap 12 relative to the pipe body 11 can be facilitated.

[0137] Of course, other structures can also be arranged in the present application to increase the friction of the outer circumferential surface of the cap 12 and the outer circumferential surface of the pipe body 11, for example, groove-shaped, protrusion-shaped or other-shaped anti-skid structures are arranged on the outer circumferential surface of the cap 12 and the outer circumferential surface of the pipe body 11.

[0138] In addition, the extraction cavity in the application can be provided with a replaceable extraction membrane, or the extraction membrane can be pre-placed in the extraction cavity to facilitate use and avoid pollution from the external environment. The extraction cavity is provided with an extraction membrane, the waste liquid cavity is communicated to the inner side of the extraction membrane, and the sample cavity is communicated to the outer side of the extraction membrane.

[0139] As described above, the reagent cavity 1201 is arranged in the cap 12 in the application, and in other embodiments of the application, a cavity can also be arranged in the tube body 11 to place reagents, or reagents can be placed in the tube body 11 and the cap 12.

[0140] As Figure 16 In some embodiments of the application, the tube body has a piston cavity 1110 and a piston rod 114, the piston cavity 1110 is communicated to the extraction cavity, and the inner end surface of the piston cavity 1110 is provided with a second puncture structure; the cap 12 is adapted to press the piston rod 114 to make the reagent in the piston cavity 1110 enter the extraction cavity. In the cap 12, reagents can be placed in the piston cavity 1110, and when the cap 12 moves in the direction parallel to the axis of the tube body 11, the cap 12 can press the piston rod 114, so that the reagent in the piston cavity 1110 enters the extraction cavity.

[0141] In the piston cavity 1110, reagents such as freeze-dried powder can be placed, and the reagent can be in powder or other forms. In addition, reagents can be placed in the cap 12 and the tube body 11, thereby improving the application range of the reagent bottle 100 in the application. In actual use, reagents in the cap 12 can be added first, and then reagents in the piston cavity 1110 can be added. That is, when the sleeve 111 punctures all the reagent cavities 1201 in the cap 12 during the movement of the cap 12, the cap 12 drives the piston rod 114 to inject the reagents in the piston cavity 1110 into the extraction cavity. Of course, the order of adding reagents in the cap 12 and reagents in the tube body 11 can be selected according to actual use.

[0142] Optionally, as Figure 16 A sealing ring 115 is arranged between the outer circumferential surface of the piston rod 114 and the inner circumferential surface of the piston cavity 1110, and the sealing ring 115 seals the gap between the piston rod 114 and the piston cavity 1110. The sealing ring 115 can seal the gap between the piston rod 114 and the piston cavity 1110, so as to prevent the reagents in the piston cavity 1110 from flowing out, and prevent foreign matters outside the piston cavity 1110 from entering the piston cavity 1110, thereby improving the stability of the reagent bottle 100.

[0143] The outer circumferential surface of the piston rod 114 is provided with a first sealing groove, and the sealing ring 115 is embedded in the first sealing groove and protrudes from the outer circumferential surface of the piston rod 114. Thus, the sealing ring 115 can be stably installed on the piston rod 114. In addition, the inner circumferential surface of the piston cavity 1110 is provided with a second sealing groove, and the part of the sealing ring 115 protruding from the outer circumferential surface of the piston rod 114 is adapted to be embedded in the second sealing ring 115 and adapted to slide out of the second sealing groove. In this way, before the piston rod 114 starts to move, the cooperation of the first sealing groove, the second sealing groove and the sealing ring 115 realizes the stable sealing between the piston rod 114 and the inner circumferential surface of the piston cavity 1110, and when the piston rod 114 is driven to move by the cap 12, the sealing ring 115 can be slid out of the second sealing groove to realize the stable extension and contraction of the piston rod 114. The piston rod 114 and the piston cavity 1110 are constructed in a structure similar to a syringe, which facilitates the injection of reagents into the extraction cavity.

[0144] Of course, in the present application, the sealing ring 115 can also be fixed on the inner circumferential surface of the sealing cavity, and the piston rod 114 is movable relative to the sealing ring 115.

[0145] In combination Figure 14 and Figure 16 In some embodiments of the present application, the cap 12 is provided with an inner tube 121 extending along the axis of the tube body 11, and the cap 12 is adapted to drive the inner tube 121 to press against the piston rod 114. Thus, the stability of driving the piston rod 114 can be improved. In combination with the foregoing embodiments, the inner tube 121 is provided with a plurality of reagent cavities 1201, so that the inner tube 121 can press and drive the piston rod 114 while constructing a plurality of reagent cavities 1201 in the cap 12, thereby optimizing and simplifying the structure of the reagent bottle 100.

[0146] Optionally, the inner tube 121 is provided with a plurality of reagent cavities 1201 spaced along the axis of the tube body 11, and the inner tube 121 is adapted to be inserted into the tube body 11 from the second end of the tube body 11, so as to sequentially pierce and communicate the plurality of reagent cavities 1201 and make the liquid reagents in the reagent cavities 1201 enter the sample cavity 1102.

[0147] As mentioned above, in combination Figures 13 to 24The reagent bottle 100 provided in the present application is characterized in that the sleeve 111 and the tube body 11 are in a concentric column shape, the hollow structure of the sleeve 111 is configured to form the sample cavity 1102, and a partition structure is arranged in the cavity between the sleeve 111 and the tube body 11 to form the piston cavity 1110, and a piston rod 114 is arranged to be telescopically inserted into the piston cavity 1110, the sleeve 111 and the tube body 11 can be integrally formed, and the partition structure configured to form the piston cavity 1110 can also be integrally formed with the sleeve 111 and the tube body 11. A second coarse thread 1104 and a fine thread are arranged on the outer peripheral surface of the second end of the tube body 11, and the fine thread cooperates with the second coarse thread. The cap 12 is rotatably sleeved on the tube body 11, and a first coarse thread 1205 is arranged on the inner peripheral surface of the cap 12, which cooperates with the second coarse thread 1104 to realize the thread transmission between the cap 12 and the tube body 11, and the fine thread on the outer peripheral surface of the tube body 11 can provide resistance to the rotation of the cap 12. A sealing rib 1204 is arranged on the inner end surface of the cap 12, and when the tube body 11 is inserted into the top of the cap 12, the second end of the tube body 11 can cooperate with the sealing rib 1204 to improve the sealing effect. An inner tube 121 is arranged in the cap 12, the inner tube 121 is in a trumpet shape, and a plurality of diaphragms 122 are arranged in the inner tube 121, which form a plurality of reagent cavities 1201 in the inner tube 121, and different reagents can be stored in the plurality of reagent cavities 1201. In use, the sample is placed in the sleeve 111, the cap 12 is covered on the tube body 11, the cap 12 is rotated, the cap 12 is opposite to the central region of the diaphragm 122, and as the cap 12 is rotated, the diaphragm 122 will touch the sleeve 111, at this time, the first coarse thread 1205 contacts the fine thread, and further rotating the cap 12, the sleeve 111 will pierce the diaphragm 122, at this time, the reagent in the reagent cavity 1201 will enter the sleeve 111 to contact and mix with the sample in the sample cavity 1102. As the cap 12 is rotated, a plurality of reagent cavities 1201 can be pierced by the sleeve 111, so as to complete the injection of a plurality of reagents.

[0148] Meanwhile, when the cap 12 moves to a predetermined position, the cap 12 will contact the piston rod 114, at this time, the piston rod 114 will provide a reverse force to the cap 12 to slow down the movement of the cap 12, so as to facilitate the sufficient mixing of the reagent and the sample, and avoid the influence of the simultaneous mixing of a plurality of reagents on the detection effect, and the reagent can also be arranged in the piston cavity 1110, so that when the cap 12 moves to a specific position, the cap 12 can drive the piston rod 114 to inject the reagent in the piston cavity 1110 into the extraction cavity.

[0149] In the present application, the extraction cavity can be placed with test paper, extraction film 118, etc.

[0150] As mentioned above, the present application describes the scheme of adding reagents into the sleeve 111, different reagents can be added into the sleeve 111 to complete the detection required. Moreover, after the final reagent added into the sleeve 111 and mixed with the sample, etc., it will enter into the extraction cavity. The following describes the situation of the reagents entering and leaving the extraction cavity in some embodiments of the present application.

[0151] In combination Figure 14 , Figures 16 and Figures 22 to 24 , in some embodiments of the present application, the sleeve 111 and the tube body 11 are configured to form a waste cavity, and the extraction cavity is in communication with the waste cavity. Wherein, the sleeve 111 is configured to form a sample cavity 1102, and the sleeve 111 is configured to form a waste cavity outside. During use, the reagent can be added into the sample cavity 1102, and the reagent can enter the extraction cavity through the sample cavity 1102, and the excess reagent can flow back to the waste cavity through the extraction cavity, so as to improve the stability of the reagent bottle 100. The extraction membrane 118 (such as for nucleic acid extraction) can be arranged in the extraction cavity. Through the extraction membrane 118, the reagent and the sample can be conveniently retained in the extraction cavity, and the excess part can enter the waste cavity, so as to avoid waste.

[0152] In some embodiments of the present application, the first end of the tube body 11 is provided with an inner end plate 112 and an outer end plate 113, and the extraction cavity is configured between the inner end plate 112 and the outer end plate 113. The first through hole 1106 and the second through hole 1113 are configured on the inner end plate 112, and the first through hole 1106 is connected to the sample cavity 1102 and the extraction cavity, and the second through hole 1113 is connected to the waste cavity and the extraction cavity. By arranging the first through hole 1106 and the second through hole 1113, the sample cavity 1102 and the waste cavity can be conveniently communicated with the extraction cavity, the reagent can flow in the sample cavity 1102, the extraction cavity and the waste cavity, the extraction of the sample can be completed, and the purpose of detecting the sample can be achieved.

[0153] Optionally, as Figure 22 and Figure 23 , the inner end plate 112 is provided with a plurality of first through holes 1106 and a plurality of second through holes 1113, the plurality of first through holes 1106 are arranged along the circumference of the inner end plate 112, and the plurality of second through holes 1113 are arranged along the circumference of the inner end plate 112, so as to improve the efficiency of the reagent entering the extraction cavity and contacting the extraction membrane 118 in the extraction cavity, and also to facilitate the reflux of the excess reagent to the waste cavity.

[0154] Further, the distance between the first through hole 1106 and the sample cavity 1102 is greater than the distance between the second through hole 1113 and the sample cavity 1102. At this time, after the reagent enters the extraction cavity, it will flow back to the waste cavity after passing through the extraction membrane 118, so as to avoid the reagent directly flowing back to the waste cavity through the extraction cavity, and to provide sufficient reaction time for the reagent and the sample.

[0155] In combination Figure 16 And Figure 21 In some embodiments of the present application, the first end of the tube body 11 is provided with an annular plate 116, the inner periphery of the annular plate 116 is along the connecting sleeve 111, the outer periphery of the annular plate 116 is along the connecting tube body 11, the outer end surface of the annular plate 116 is provided with a plurality of first guide grooves 1119, the plurality of first guide grooves 1119 are all communicated with the sample cavity 1102 and extend in a diverging manner away from the sample cavity 1102, and the first guide grooves 1119 are communicated with the first through hole 1106. Thus, the time and distance of the reagent flowing in the process of entering and discharging the extraction cavity can be increased, more reaction time is provided for the reagent and the sample, and the extraction effect is enhanced.

[0156] Optionally, in combination Figure 16 And Figure 21 The annular plate 116 is provided with a third through hole 1114, the third through hole 1114 is spaced apart from the first guide groove 1119, and the third through hole 1114 is opposite to the second through hole 1113. The second through hole 1113 cooperates with the third through hole 1114 to connect the extraction cavity and the waste liquid cavity. The communication between the extraction cavity and the waste liquid cavity is facilitated, excessive pressure of the reagent in the extraction cavity is avoided, and subsequent other reagents can quickly enter the extraction cavity.

[0157] Optionally, as Figure 23 The outer side surface of the inner end plate 112 is provided with a containing groove 1115, and the outer end plate 113 covers the containing groove 1115 to construct the extraction cavity. The bottom surface of the containing groove 1115 is provided with longitudinally and transversely intersecting flow-through grooves 1116, and the second through hole 1113 connects the flow-through grooves 1116. The extraction membrane 118 and the like can be conveniently placed in the extraction cavity, and the coverage of the reagent in the extraction cavity is further increased to facilitate the extraction of the extraction membrane 118.

[0158] In combination Figure 23 It can be seen that the containing groove 1115 is in the shape of a circular groove, and the bottom surface of the containing groove is provided with a circular flow-through groove 1116. The flow-through groove 1116 is provided with a plurality of annular ribs arranged in a spaced-apart manner from inside to outside, and a plurality of radial grooves are guided through the plurality of annular ribs. Thus, the reagent can uniformly flow to the extraction membrane in the extraction cavity.

[0159] As Figure 24 In some embodiments of the present application, the inner side surface of the outer end plate 113 is provided with a central groove 1117 and a second guide groove 1118. The central groove 1117 constructs part of the extraction cavity, the second guide groove 1118 is communicated with the central groove 1117 and extends in a diverging manner away from the central groove 1117, and the second guide groove 1118 is communicated with the first through hole 1106.

[0160] The reagent bottle 100 according to the embodiment of the application has a structure of multiple layers of membranes 122 separating reagents, and is suitable for detection reactions requiring reagents to be added in batches. For example, nucleic acid detection of the new coronavirus.

[0161] There are various modes in specific technical solutions: (1) the multi-layer design shown in the figure, the rotating cap 12 is used to add reagents by gradually piercing; (2) different reagents are sealed in the same plane, the first piercing structure is designed at the edge position, the first reagent is pierced by the downward displacement of the cap 12, and the piercing of other reagent chambers 1201 can be achieved by rotating the cap 12 to gradually pierce the reagent separation membranes 122. Figure 14

[0162] The reagent bottle 100 in the application can be provided with a rotating scale on the bottle body to prompt the type of reagent to be added.

[0163] The application stores multiple-component reagents in a reaction-integrated tube, and each component is stored in a container and separated by a “splitting sheet” (which can be a film or a sheet) to avoid cross-contamination. The position of each component reagent in the tube is set according to experimental needs. The number of reagent components is set according to experimental needs. After the sample is opened, it can be placed in the sleeve 111, and then the cap is screwed on. When in use, the sleeve 111 pierces the membranes 122 of different components of reagents one by one by screwing or pressing to displace the sleeve 111, so that the reagents of different components flow into the sleeve 111 in sequence, pass through the sample, and then enter the extraction chamber for reaction. The reagent components that do not need to flow through the sample can flow into the extraction chamber through a separate microchannel. The extraction chamber is connected to the sample chamber 1102 and the waste liquid chamber through a micro-pipe. The micro-pipe has multiple groups (multiple first through holes 1106 and multiple second through holes 1113), which can achieve large-particle filtration and prevent blockage of a certain component.

[0164] An extraction membrane 118 can be arranged in the extraction chamber. The extraction membrane 118 can be a sheet (the optimized height is 0.2 mm), which has a large heat receiving area and uniform heat conduction, thereby shortening the reaction time of PCR. For a particulate sample, the focal plane of the microscopic image is stable. A step is designed in the cap 12, which corresponds to the liquid level of each reagent component. When the cap reaches this position, the resistance increases, which serves as a position reminder for manual operation. The outer circumferential surface of the tube body 11 can be printed with a position line of the reagent components, which serves as a position reminder for manual operation.

[0165] The reagent bottle 100 in the application can be operated manually or on a machine.

[0166] ​The pipe body 11 and the pipe cap 12 in the application have a sealing design, and the pipe body 11 and the pipe cap 12 are sealed after the last kind of component reagent flows into the sleeve 111, all reagents are sealed into the pipe to avoid leakage. The waste liquid cavity can be filled with water absorption materials (filter paper or sponge, etc.), and when the waste liquid enters the waste liquid cavity, it is adsorbed by the water absorption material to prevent backflow into the micro-pipe.

[0167] In the application, any auxiliary tool is not needed, and nucleic acid extraction is realized.

[0168] The PCR reagent bottle 100 in the application focuses on the structure of the liquid flow channel and the small chamber for dispensing enzyme and eluent, and the PCR reaction chamber. It is suitable for directly adding the extracted nucleic acid. The dispensing enzyme in each reagent cavity 1201 in the application adopts a freeze-drying method, and each reagent cavity 1201 in the application can be respectively provided with a sample preservation liquid, a lysis liquid, a washing liquid 1, a washing liquid 2, an eluent, and an enzyme reaction system. The extraction cavity in the application can be used as a PCR reaction chamber, and the reaction liquid can enter the extraction cavity from the sample cavity 1102 or enter the waste liquid cavity from the extraction cavity.

[0169] The reaction can be promoted by heating the extraction membrane in the extraction cavity at different temperatures, and detection can be realized by using different filters.

[0170] In addition, the application also provides a detection system, which comprises a positioning module, a heating module, a shooting module, and a reagent bottle. The reagent bottle is the reagent bottle according to the foregoing embodiments, wherein the positioning module has an insertion port, the first end of the reagent bottle is adapted to be inserted into the insertion port and positioned by the positioning module, the heating module is used for heating the extraction cavity, and the shooting module is opposite to the end face of the first end of the reagent bottle to be adapted to obtain a fluorescence quantitative image.

[0171] According to the detection system of the embodiment of the application, the addition of various reagents can be realized in a closed tube, and after the addition of the various reagents, the reaction and nucleic acid extraction and the like can be carried out in the closed pipe body. In addition, the reagent bottle can also be used as a reaction cavity. In the process of obtaining a fluorescence quantitative image, the extraction membrane does not need to be taken out, but can be directly obtained and reacted, so that the detection precision is effectively improved, and the pollution to the external environment and the influence of the external environment on the detection process are avoided.

[0172] Although the embodiments of the application have been shown and described above, it should be understood that the above embodiments are exemplary and should not be construed as limiting the application. Those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the application.

Claims

1. A reagent bottle, characterized in that, include: The tube body has a sleeve inside, a sample chamber is constructed inside the sleeve, a waste liquid chamber is constructed between the sleeve and the tube body, and an extraction chamber is provided at the first end of the tube body. The waste liquid chamber and the sample chamber are both connected to the extraction chamber through a microchannel. The tube body has a first puncture structure. A cap, which covers the second end of the tube body, has a closed reagent cavity inside. The cap is movably connected to the tube body to allow the first piercing structure to pierce the reagent cavity and connect it to the cannula for closed-tube extraction. The tube contains a piston chamber and a piston rod. The piston chamber is connected to the extraction chamber, and the inner end face of the piston chamber is provided with a second puncture structure.

2. The reagent bottle according to claim 1, characterized in that, The cap is provided with multiple reagent chambers at intervals, and the multiple reagent chambers are adapted to be punctured sequentially by the first puncture structure and connected to the sleeve in sequence.

3. The reagent bottle according to claim 2, characterized in that, The cap is movably connected to the tube body along the axial direction of the tube body, and the plurality of reagent chambers are spaced apart along the axial direction of the cap.

4. The reagent bottle according to claim 3, characterized in that, The cap contains an inner tube that extends along the axis of the tube body. The end of the inner tube away from the sleeve is closed and the end facing the sleeve is open. The inner tube contains a plurality of diaphragms that are spaced apart along the axis of the tube body. A reagent chamber is formed between each pair of adjacent diaphragms and between each diaphragm and the closed end of the inner tube.

5. The reagent bottle according to claim 4, characterized in that, The diaphragm includes a rigid membrane layer and a flexible membrane layer. The rigid membrane layer is connected around the flexible membrane layer, and the rigid membrane layer and the flexible membrane layer are connected to form a flat plate. The flexible membrane layer and the sleeve are opposite to each other in the axial direction of the tube body, and the thickness of the flexible membrane layer is lower than the thickness of the rigid membrane layer.

6. The reagent bottle according to claim 5, characterized in that, The flexible membrane layer and the rigid membrane layer are configured as a recessed structure with the opening facing the sleeve; and / or The surface of the flexible membrane layer is provided with multiple strip grooves, which are connected at one point and arranged in a divergent pattern.

7. The reagent bottle according to claim 4, characterized in that, The inner tube is configured as a variable-diameter tube with a radial dimension that gradually decreases in the direction away from the sleeve, and multiple steps are formed on the inner circumferential surface of the inner tube. The diaphragm is disposed inside the sleeve and supported on the steps on the inner circumferential surface of the inner tube.

8. The reagent bottle according to claim 4, characterized in that, The inner end face of the cap is provided with an annular groove, and the end of the inner tube is provided with an annular hook, which engages with the annular groove.

9. The reagent bottle according to claim 2, characterized in that, The cap is rotatably connected to the tube body, and a plurality of reagent chambers are spaced apart circumferentially along the cap.

10. The reagent bottle according to any one of claims 2-9, characterized in that, The cap contains, in sequence along the puncture sequence of the first puncture structure, sample preservation solution, lysis solution, first cleaning solution, second cleaning solution, and air column.

11. The reagent bottle according to any one of claims 1-9, characterized in that, The end of the sleeve is configured with the first puncture structure, and the end of the sleeve is provided with a plurality of notches spaced apart along the circumference of the sleeve.

12. The reagent bottle according to any one of claims 1-9, characterized in that, The cap is threaded into the tube body.

13. The reagent bottle according to claim 12, characterized in that, The cap is fitted onto the outside of the tube body. The inner circumferential surface of the cap is provided with a first coarse thread, and the outer circumferential surface of the tube body is provided with a second coarse thread. The first coarse thread and the second coarse thread mate. The outer circumferential surface of the tube is also provided with fine threads, which mate with the second coarse thread, and the first coarse thread contacts the fine thread when the sleeve punctures the reagent cavity.

14. The reagent bottle according to claim 1, characterized in that, The inner end face of the cap is provided with a sealing rib, which is adapted to abut against the inner circumference of the second end of the pipe body so that the cap and the pipe body are sealed together.

15. The reagent bottle according to claim 1, characterized in that, An anti-detachment groove is provided on the outer circumferential surface of the first end of the pipe body, and the anti-detachment groove is arranged along the circumference of the pipe body; and / or The outer circumferential surface of the cap is provided with multiple grooves, the grooves being parallel to the axis of the pipe body, and the multiple grooves being spaced apart circumferentially along the cap; and / or The outer circumferential surface of the first end of the tube is provided with anti-slip ribs, which extend in a direction parallel to the axis of the tube and are provided at intervals along the circumference of the tube.

16. The reagent bottle according to claim 1, characterized in that, The extraction chamber is equipped with an extraction membrane, the waste liquid chamber is connected to the inner side of the extraction membrane, and the sample chamber is connected to the outer side of the extraction membrane.

17. A detection system, characterized in that, The detection system includes: a positioning module, a heating module, an imaging module, and a reagent bottle, wherein the reagent bottle is the reagent bottle according to any one of claims 1-16. The positioning module has an insertion port, the first end of the reagent bottle is adapted to be inserted into the insertion port and positioned by the positioning module, the heating module is used to heat the extraction chamber, and the imaging module is opposite to the end face of the first end of the reagent bottle to acquire a quantitative fluorescence image.

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