A supercritical extraction process of ganoderma lucidum spore oil

Abstract

The application discloses a ganoderma lucidum spore oil supercritical extraction process and relates to the technical field of traditional Chinese medicine extraction. The ganoderma lucidum spore oil supercritical extraction process specifically comprises the following steps: after ganoderma lucidum spore powder and wall-breaking auxiliary agent particles are uniformly stirred and mixed, the wall-breaking auxiliary agent particles are separated out by mechanical wall breaking, and wall-broken ganoderma lucidum spore powder is obtained; the wall-broken ganoderma lucidum spore powder is added into a stirrer, deionized water is then added, granulation is performed by using a granulator, and drying is performed until the water content is less than 5%, so that ganoderma lucidum spore powder particles are obtained; the ganoderma lucidum spore powder particles are added into a supercritical carbon dioxide extraction device for extraction, and ganoderma lucidum spore oil is obtained. The ganoderma lucidum spore oil supercritical extraction process adds wall-breaking auxiliary agent particles in the process of mechanical wall breaking, the collision effect between the wall-breaking auxiliary agent particles and the ganoderma lucidum spore powder is improved, the wall breaking efficiency is improved to a certain extent, and the wall-broken ganoderma lucidum spore powder can be prevented from being oxidized and deteriorated to a certain extent.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine extraction, and particularly relates to a supercritical extraction process of ganoderma spore oil. BACKGROUND

[0002] Ganoderma is the dried fruiting body of Ganoderma lucidum (Leyss.ex Fr.) Karst. or G. sinense Zhao, Xu et Zhang. It has the effects of analgesia, sedation and detoxification, and can resist tumors, regulate immunity and reduce blood sugar, and has the effects of nourishing and invigorating the body and prolonging life. Ganoderma spores are the essence of ganoderma, and are rich in a large amount of bioactive substances, including lipids, vitamins, triterpenes, alkaloids, polysaccharides, sterols, inorganic ions, proteins and amino acids. Data shows that ganoderma spore powder has extensive medical effects. Ganoderma spore oil is an oily lipid extracted from ganoderma spores by an extraction technology, and is mainly composed of saturated fatty acids and unsaturated fatty acids and various active components of ganoderma spores, and is a yellow transparent liquid. Research proves that ganoderma spore oil has the functions of improving immunity and resisting oxidation of the body, and has good health care effects.

[0003] However, under an optical microscope, ganoderma spores are elliptical, have a diameter of 5-8 μm, and have a double-layer cell wall, which is mainly composed of chitinous acid, lignin, cellulose and metal ions such as Si, Ca, Fe and Mg. The spore cell wall is very hard and can resist acid, alkali, high temperature and digestive enzymes. Therefore, if ganoderma spores are directly extracted, the extraction rate is low. Therefore, before the extraction of ganoderma spore oil, the ganoderma spore powder often needs to be broken.

[0004] Mechanical breaking is the most widely used breaking method in the prior art, which grinds the ganoderma spore powder by means of machinery, so that the cell wall of the ganoderma spore powder is broken. However, in the grinding process, the temperature rapidly increases due to high-speed impact, which causes the ganoderma spore powder to be oxidized due to high temperature, and thus the efficacy of the ganoderma spore powder is greatly discounted. SUMMARY

[0005] In view of the above problems, the present application aims to disclose a supercritical extraction process of ganoderma spore oil. In the process of mechanical breaking, a breaking aid particle is added, which can improve the collision effect between the breaking aid particle and the ganoderma spore powder, improve the breaking efficiency to a certain extent, and prevent the broken ganoderma spore powder from being oxidized and deteriorated to a certain extent.

[0006] Specifically, the supercritical extraction process of ganoderma spore oil comprises the following steps:

[0007] Breaking wall: after the Ganoderma lucidum spore powder and the wall breaking aid particles are stirred and mixed uniformly, mechanical breaking wall is performed, the wall breaking aid particles are separated, and the broken wall Ganoderma lucidum spore powder is obtained;

[0008] Granulation: the broken wall Ganoderma lucidum spore powder is added into a stirrer, then deionized water is added, a granulator is used for granulation, and drying is performed until the water content is less than 5%, so that the Ganoderma lucidum spore powder particles are obtained;

[0009] Supercritical extraction: the Ganoderma lucidum spore powder particles are added into a supercritical carbon dioxide extraction device for extraction, so that Ganoderma lucidum spore oil is obtained.

[0010] Further, the mass ratio of the Ganoderma lucidum spore powder and the wall breaking aid particles is 10: (1-1.5).

[0011] Further, in the granulation step, the mass ratio of the broken wall Ganoderma lucidum spore powder and the deionized water is 1: (0.4-0.6).

[0012] Further, the temperature of the supercritical extraction is 40-50℃, the pressure is 25-30MPa, and the extraction time is 60-100min.

[0013] Further, the wall breaking aid particles are obtained by mixing and sintering titanium nitride and porous zirconium oxide to obtain a porous carrier, and then loading nano zero-valent iron.

[0014] Further, the particle size of the wall breaking aid particles is 1-3μm.

[0015] Further, the preparation method of the wall breaking aid particles specifically includes the following steps:

[0016] S1: acrylamide and ammonium polyacrylate are added into deionized water and stirred to obtain a mixed solution; porous zirconium oxide and nano titanium nitride particles are placed in a ball mill, ball-mixed, and then the mixed solution is added for ball-mixing for 5-6h; PVA, polyethylene glycol and sodium dodecyl sulfate are added and continuously ball-mixed for 20-24h, so that a mixed slurry is obtained; the mixed slurry is placed in a reaction kettle, ammonium persulfate and tert-butyl alcohol are added, stirred for 20-30min, and then left for 5-10min; spray granulation is performed, so that a composite particle blank is obtained;

[0017] S2: the prepared composite particle blank is placed in a muffle furnace, heated to 200-400℃ at a rate of 2-4℃ / min, kept for 1-2h, then heated to 1500-1550℃ at a rate of 5-8℃ / min, kept for 5-6h, and then cooled to 300-400℃ at a rate of 2-3℃ / min, and cooled to room temperature in the furnace, so that a porous carrier is obtained;

[0018] S3: take the iron sulfate heptahydrate into the deionized water, after stirring and dissolving, add the porous carrier, ultrasonic dispersion, then add the sodium carboxymethyl cellulose solution, magnetic stirring for 3-4h, then add the sodium tetrahydroborate solution dropwise under stirring, after the dropwise addition is completed, continue stirring until no bubbles are generated in the reaction solution, filter, the filter cake is washed with deionized water and anhydrous ethanol alternately for three times, vacuum drying, to obtain the wall-breaking aid particles.

[0019] The wall-breaking aid particles of the present application, in the process of sintering, are first slowly heated to 200-400 DEG C, pre-sintering is carried out to prevent the temperature from changing too fast and the embryo from cracking too fast, and when loading the nano zero-valent iron, the sodium carboxymethyl cellulose can be coated on the surface of the generated nano zero-valent iron, which can increase the bonding force between the nano zero-valent iron and the porous carrier, and can also protect the nano zero-valent iron to some extent, reducing the probability of oxidation during storage.

[0020] Further, the mass ratio of the porous zirconium oxide and the nano titanium nitride is 5:(1-1.3).

[0021] Further, the mass ratio of the porous carrier and the iron sulfate heptahydrate is 1:(2-3).

[0022] The present application has the following advantages:

[0023] 1. The present application discloses a supercritical extraction process of ganoderma spore oil, in the process of mechanical wall breaking, wall-breaking aid particles are added, which can increase the collision between the ganoderma spore powder, thereby improving the efficiency of mechanical wall breaking to some extent, on the other hand, the wall-breaking aid of the present application, as the carrier, the zirconium oxide and the titanium nitride have good thermal conductivity, which can timely conduct the heat generated in the wall breaking process, thereby avoiding the temperature of the ganoderma spore powder being too high, and secondly, the loaded nano zero-valent iron can inhibit the oxidation of the ganoderma spore powder to some extent, thereby ensuring the activity of the effective ingredients in the wall broken ganoderma spore powder.

[0024] 2. The wall-breaking aid particles of the present application, after mixing and sintering of the zirconium oxide and the titanium nitride as the carrier, have high hardness, which can avoid fragmentation during mechanical wall breaking, and the loaded nano zero-valent iron endows the wall-breaking aid particles with magnetism, which can be easily separated by an external magnetic field after use, avoiding residue. DETAILED DESCRIPTION

[0025] The present application will be described in detail below with specific examples:

[0026] The supercritical extraction process of ganoderma spore oil of the present application is as follows:

[0027] Example 1

[0028] Preparation of wall-breaking aid particles

[0029] S1: Acrylamide and ammonium polyacrylate were taken in a mass ratio of 7:1, added to 20 times the mass of deionized water of acrylamide, stirred and mixed to obtain a mixed solution, porous zirconium oxide and nano titanium nitride particles were taken in a mass ratio of 5:1.3, respectively, and placed in a ball mill, stirred and mixed after ball milling, then the mixed solution was added for ball milling and mixing for 6h, then PVA, polyethylene glycol and sodium dodecyl sulfate were added, the mass ratio of PVA, polyethylene glycol and sodium dodecyl sulfate to porous zirconium oxide was 0.1:0.02:0.05:1, and the ball milling and mixing was continued for 24h to obtain a mixed slurry, wherein the solid content in the mixed slurry was 55%, the mixed slurry was placed in a reaction kettle, 0.12 times the mass of the mixed slurry of ammonium persulfate and 0.01 times the mass of the mixed slurry of tert-butyl alcohol were added, stirred for 25min, and then left to stand for 10min, then spray granulation was performed to obtain a composite particle blank;

[0030] S2: The composite particle blank prepared was placed in a muffle furnace, heated to 400℃ at a rate of 3℃ / min, kept at 400℃ for 1h, then heated to 1500℃ at a rate of 5℃ / min, and kept at 1500℃ for 5h of calcination, then cooled to 300℃ at a rate of 3℃ / min, and cooled to room temperature in the furnace to obtain a porous carrier;

[0031] S3: Iron sulfate heptahydrate was taken and added to deionized water, stirred and dissolved to obtain a 0.1mol / L iron sulfate solution, then the porous carrier was added, the mass ratio of the porous carrier to the iron sulfate heptahydrate was 1:2, ultrasonic dispersion was performed, then 1 / 2 times the volume of the iron sulfate solution of a 2g / ml carboxymethyl cellulose sodium solution was added, magnetic stirring was performed for 4h, then 0.4mol / L sodium tetrahydroborate solution was added at a rate of 3ml / min under stirring, the total molar ratio of the added sodium tetrahydroborate to the iron sulfate heptahydrate was 1:2, after the addition was completed, stirring was continued until no gas bubbles were generated in the reaction solution, then filtration was performed, the filter cake was washed with deionized water and anhydrous ethanol alternately for three times, and vacuum drying was performed to obtain wall-breaking aid particles, which were detected to have a particle size of 1-3μm.

[0032] Supercritical extraction of ganoderma spore powder was performed using the prepared wall-breaking aid particles, which included the following steps:

[0033] Wall breaking: The ganoderma spore powder and the wall-breaking aid particles were stirred and mixed in a mass ratio of 10:1.5, then mechanical wall breaking was performed, the wall-breaking aid particles were magnetically separated, and wall-broken ganoderma spore powder was obtained, which was detected by microscopy to have a wall breaking rate of 98.9%.

[0034] Granulation: the broken wall ganoderma spore powder is added into a blender, then 0.5 times the mass of the broken wall ganoderma spore powder of deionized water is added, granulation is performed by using a granulator, and the granulation is passed through a 20-mesh sieve, dried to a water content of less than 5%, and ganoderma spore powder granules are obtained.

[0035] Supercritical extraction: the ganoderma spore powder granules are added into a supercritical carbon dioxide extraction device, extraction is performed at a temperature of 40℃ and a pressure of 25MPa for 100min, and golden yellow and transparent ganoderma spore oil is obtained.

[0036] Example Two

[0037] Preparation of broken wall auxiliary granules

[0038] S1: acrylamide and ammonium polyacrylate are taken according to a mass ratio of 7:3, stirred and uniformly mixed in 25 times the mass of acrylamide of deionized water to obtain a mixed solution, porous zirconium oxide and nano titanium nitride granules are taken according to a mass ratio of 5:1, placed in a ball mill, ball-mixed uniformly, then the mixed solution is added for ball-mixing for 5h, PVA, polyethylene glycol and sodium dodecyl sulfate are added, and the mass ratio of PVA, polyethylene glycol and sodium dodecyl sulfate to porous zirconium oxide is 0.12:0.02:0.08:1, and the ball-mixing is continued for 22h to obtain a mixed slurry, wherein the solid content in the mixed slurry is 65%, the mixed slurry is placed in a reaction kettle, 0.1 times the mass of the mixed slurry of ammonium persulfate and 0.015 times the mass of the mixed slurry of tert-butyl alcohol are added, stirred and reacted for 20min, left to stand for 8min, and spray-granulated to obtain a composite granule blank;

[0039] S2: the composite granule blank prepared is placed in a muffle furnace, heated at a rate of 4℃ / min to 300℃, kept at 300℃ for 2h, then heated at a rate of 6℃ / min to 1550℃, kept at 1550℃ for calcination for 6h, cooled at a rate of 3℃ / min to 300℃, and cooled to room temperature with the furnace, and a porous carrier is obtained;

[0040] S3: iron sulfate heptahydrate is taken and dissolved in deionized water to obtain a 0.1mol / L iron sulfate solution, the porous carrier is added, and the mass ratio of the porous carrier to the iron sulfate heptahydrate is 1:3, ultrasonic dispersion is performed, 1 / 2 times the volume of the iron sulfate solution of a 2.5g / ml carboxymethyl cellulose sodium solution is added, magnetic stirring is performed for 4h, then a 0.4mol / L sodium tetrahydroborate solution is added at a speed of 5ml / min under stirring, the total molar ratio of the added sodium tetrahydroborate to the iron sulfate heptahydrate is 1:2, after the addition is completed, stirring is continued until no gas bubbles are generated in the reaction solution, suction filtration is performed, the filter cake is washed with deionized water and anhydrous ethanol alternately for three times, and vacuum drying is performed to obtain broken wall auxiliary granules, and detection shows that the particle size of the broken wall auxiliary granules is 1-3μm.

[0041] The prepared broken wall auxiliary particles are used for supercritical extraction of ganoderma spore powder, which specifically comprises the following steps:

[0042] Breaking wall: the ganoderma spore powder and the broken wall auxiliary particles are stirred and mixed according to a mass ratio of 10:1.2, and then mechanical breaking wall is performed, and the broken wall auxiliary particles are separated by magnetism to obtain broken wall ganoderma spore powder. The breaking rate of the broken wall ganoderma spore powder is 99.3% by microscopic examination.

[0043] Granulation: the broken wall ganoderma spore powder is added into a stirrer, and then 0.4 times the mass of the broken wall ganoderma spore powder of deionized water is added, and granulation is performed by using a granulator, and then the granules are sieved through a 20-mesh sieve, and dried until the water content is less than 5% to obtain ganoderma spore powder granules.

[0044] Supercritical extraction: the ganoderma spore powder granules are added into a supercritical carbon dioxide extraction device, and extraction is performed at a temperature of 45℃ and a pressure of 30MPa for 90min to obtain golden yellow and transparent ganoderma spore oil.

[0045] Example three

[0046] Preparation of broken wall auxiliary particles

[0047] S1: acrylamide and ammonium polyacrylate are taken according to a mass ratio of 8:1, and stirred and mixed in 20 times the mass of acrylamide of deionized water to obtain a mixed solution, and porous zirconium oxide and nano titanium nitride particles are taken according to a mass ratio of 5:1.2, respectively, and placed in a ball mill, and stirred and mixed after ball milling, and then the mixed solution is added for ball milling and mixing for 6h, and then PVA, polyethylene glycol and sodium dodecyl sulfate are added, and the mass ratio of PVA, polyethylene glycol and sodium dodecyl sulfate to porous zirconium oxide is 0.11:0.01:0.03:1, and the ball milling and mixing is continued for 24h to obtain a mixed slurry, wherein the solid content in the mixed slurry is 60%, and the mixed slurry is placed in a reaction kettle, and 0.11 times the mass of the mixed slurry of ammonium persulfate and 0.012 times the mass of the mixed slurry of tert-butyl alcohol are added, and stirred for 30min, and then placed for 5min, and then spray granulation is performed to obtain a composite particle blank;

[0048] S2: the prepared composite particle blank is placed in a muffle furnace, and heated to 200℃ at a rate of 2℃ / min, and then kept at 200℃ for 1h, and then heated to 1550℃ at a rate of 8℃ / min, and then kept at 1550℃ for 5h, and then cooled to 400℃ at a rate of 2℃ / min, and then cooled to room temperature in the furnace to obtain a porous carrier;

[0049] S3: Take ferric sulfate heptahydrate into deionized water, after stirring and dissolving, 0.1 mol / L ferric sulfate solution is obtained, then porous carrier is added, the mass ratio of porous carrier and ferric sulfate heptahydrate is 1:3, after ultrasonic dispersion, 1 / 2 volume of ferric sulfate solution of 2.2 g / ml sodium carboxymethyl cellulose solution is added, magnetic stirring for 3 h, then 0.4 mol / L sodium tetrahydroborate solution is added dropwise at a speed of 4 ml / min under stirring, the total molar ratio of sodium tetrahydroborate to ferric sulfate heptahydrate is 1:2, after the dropwise addition is completed, continuous stirring is carried out until no gas bubbles are generated in the reaction solution, then filtration is carried out, the filter cake is washed with deionized water and anhydrous ethanol alternately for three times, vacuum drying is carried out, and the wall-breaking adjuvant particles are obtained, and the particle size of the wall-breaking adjuvant particles is 1-3 μm.

[0050] The wall-breaking adjuvant particles prepared are used for supercritical extraction of ganoderma spore powder, and the specific steps include the following:

[0051] Wall breaking: the ganoderma spore powder and the wall-breaking adjuvant particles are stirred and uniformly mixed according to a mass ratio of 10:1, mechanical wall breaking is carried out, the wall-breaking adjuvant particles are magnetically separated, and the wall-broken ganoderma spore powder is obtained, and the wall breaking rate of the wall-broken ganoderma spore powder is 99.5% through microscopic examination.

[0052] Granulation: the wall-broken ganoderma spore powder is added into a stirring machine, then 0.6 times the mass of the wall-broken ganoderma spore powder of deionized water is added, a granulator is used for granulation, a 20-mesh sieve is used, and drying is carried out until the water content is less than 5%, and the ganoderma spore powder granules are obtained.

[0053] Supercritical extraction: the ganoderma spore powder granules are added into a supercritical carbon dioxide extraction device, extraction is carried out at a temperature of 50℃ and a pressure of 28 MPa for 60 min, and the golden yellow and transparent ganoderma spore oil is obtained.

[0054] Comparative Example 1

[0055] The difference between the present comparative example and Example 1 is that the wall-breaking adjuvant particles of the present comparative example are not loaded with nano zero-valent iron, and the wall-broken ganoderma spore powder obtained has a wall breaking rate of 98.7% through microscopic examination of the wall-broken ganoderma spore powder.

[0056] Comparative Example 2

[0057] The difference between the present comparative example and Example 1 is that the wall-breaking adjuvant particles are not added in the present comparative example, and the wall-broken ganoderma spore powder obtained has a wall breaking rate of 82.4% through microscopic examination of the wall-broken ganoderma spore powder.

[0058] The antioxidant activity of the ganoderma spore oil prepared in Example 1, Comparative Example 1 and Comparative Example 2 is determined, and the determination method is as follows:

[0059] DPPH radical scavenging ability test: 2 mL of Ganoderma lucidum spore oil was added to 2 mL of 0.4 mmol / L DPPH ethanol solution, and placed in the dark for 30 min. The absorbance A was measured at a wavelength of 517 nm i . Inhibition rate = [1-(A i -A j ) / A c ] x 100%, A c is the absorbance of the mixture of DPPH solution and anhydrous ethanol solution, and A j is the absorbance of the mixture of the extract and anhydrous ethanol solution. The test results are expressed as the half-inhibitory concentration EC 50 .

[0060] ABTS radical scavenging ability: 100 μL of Ganoderma lucidum spore oil was added to 2 mL of ABTS + working solution, and placed in the dark at room temperature for 6 min. The absorbance A was measured at a wavelength of 734 nm i . Inhibition rate = [1-(A i -A j ) / A c ] x 100%, A c is the absorbance of the mixture of anhydrous ethanol and ABTS + working solution, and A j is the absorbance of the mixture of the extract and 80% ethanol solution. The test results are expressed as the half-inhibitory concentration EC 50 .

[0061] Hydroxyl radical scavenging ability: 200 μL of 8.8 mmol / L H2O2, 200 μL of 9 mmol / L FeSO4, 200 μL of 9 mmol / L salicylic acid ethanol solution, 200 μL of Ganoderma lucidum spore oil, and 2200 μL of water were added to a test tube, mixed, and allowed to stand for 10 min. The absorbance A was measured at a wavelength of 510 nm i . Inhibition rate = [1-(A i -A j ) / A c ] x 100%, A c is the absorbance of the mixture solution without the sample, and A j is the absorbance of the mixture solution without H2O2. The test results are expressed as the half-inhibitory concentration EC 50 .

[0062] The test results are shown in Table 1:

[0063] Table 1 Antioxidant activity test results

[0064] Sample DPPH inhibition rate (mg / ml) ABTS inhibition rate (mg / ml) Hydroxyl radical inhibition rate (mg / ml) Example 1 1.68 5.32 22.89 Comparative Example 1 0.89 2.09 8.86 Comparative Example 2 1.27 3.18 14.27

[0065] It can be seen from the above, i.e., the data in Table 1, that the breakage rate of ganoderma spore powder can be effectively improved by using the breakage aid granules of the present application, and the breakage aid granules of the present application can protect the antioxidant activity of the effective components in the ganoderma spore powder.

[0066] The above examples are only used to illustrate the technical solutions of the present application but not limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or equivalently replaced without departing from the purpose and scope of the present application, and all of them should be covered in the scope of the claims of the present application. The technical, shape and structure parts not described in detail in the present application are all known technologies.

Claims

1. A supercritical extraction process of Ganoderma spore oil, characterized in that, Specifically comprising the following steps: Breaking the wall: after the Ganoderma lucidum spore powder and the wall breaking aid particles are stirred and mixed uniformly, mechanical breaking is performed, the wall breaking aid particles are separated out, and the broken wall Ganoderma lucidum spore powder is obtained; Granulation: the broken wall Ganoderma lucidum spore powder is added into a stirrer, then deionized water is added, a granulator is used for granulation, and drying is performed until the water content is less than 5%, so that the Ganoderma lucidum spore powder particles are obtained; Supercritical extraction: the Ganoderma lucidum spore powder particles are added into a supercritical carbon dioxide extraction device for extraction, so that the Ganoderma lucidum spore oil is obtained; The preparation method of the wall breaking aid particles specifically comprises the following steps: S1: acrylamide and ammonium polyacrylate are added into deionized water to stir and mix uniformly to obtain a mixed solution, porous zirconium oxide and nano titanium nitride particles are placed in a ball mill, after ball milling and mixing, the mixed solution is added for ball milling and mixing for 5-6 h, then PVA, polyethylene glycol and sodium dodecyl sulfate are added, and ball milling and mixing are continuously performed for 20-24 h, so that a mixed slurry is obtained, which is placed in a reaction kettle, ammonium persulfate and tert-butyl alcohol are added, stirring reaction is performed for 20-30 min, standing is performed for 5-10 min, spray granulation is performed, and a composite particle blank is obtained; S2: the composite particle blank prepared is placed in a muffle furnace, heating is performed at a rate of 2-4 ℃ / min to 200-400 ℃, heat preservation is performed for 1-2 h, then heating is performed at a rate of 5-8 ℃ / min to 1500-1550 ℃, heat preservation calcination is performed for 5-6 h, after calcination is completed, cooling is performed at a rate of 2-3 ℃ / min to 300-400 ℃, and the furnace is cooled to room temperature, so that a porous carrier is obtained; S3: iron sulfate heptahydrate is taken and added into deionized water, after stirring and dissolving, the porous carrier is added, ultrasonic dispersion is performed, then sodium carboxymethyl cellulose solution is added, magnetic stirring is performed for 3-4 h, then sodium tetrahydroborate solution is added dropwise in a stirring state, after dropwise addition is completed, stirring is continuously performed until no gas bubbles are generated in the reaction solution, filtration is performed, the filter cake is washed with deionized water and anhydrous ethanol alternately for three times, and vacuum drying is performed, so that the wall breaking aid particles are obtained.

2. A process for supercritical extraction of ganoderma spore oil as claimed in claim 1, wherein, The mass ratio of the Ganoderma lucidum spore powder and the wall breaking aid particles is 10:(1-1.5).

3. The supercritical extraction process of ganoderma spore oil according to claim 1, characterized in that, In the granulation step, the mass ratio of the broken wall Ganoderma lucidum spore powder and deionized water is 1:(0.4-0.6).

4. The supercritical extraction process of ganoderma spore oil according to claim 1, characterized in that, The temperature of the supercritical extraction is 40-50 ℃, the pressure is 25-30 MPa, and the extraction time is 60-100 min.

5. The supercritical extraction process of ganoderma spore oil according to any one of claims 1-4, characterized in that, The wall breaking aid particles are obtained by mixing and sintering titanium nitride and porous zirconium oxide to obtain a porous carrier, and then loading nano zero-valent iron.

6. A process for supercritical extraction of ganoderma spore oil as claimed in claim 5 wherein, The particle size of the wall breaking aid particles is 1-3 μm.

7. The supercritical extraction process of ganoderma spore oil according to claim 1, characterized in that, The mass ratio of the porous zirconium oxide and the nano titanium nitride is 5:(1-1.3).

8. The supercritical extraction process of ganoderma spore oil according to claim 1, characterized in that, The mass ratio of the porous carrier and iron sulfate heptahydrate is 1:(2-3).