Compositions for improving the color of aqueous suspensions containing guar and methods of making and using the same

Abstract

A composition for improving the color of aqueous suspensions containing guar gum, and methods of making and using the same, are disclosed. The composition comprises guar gum and an ethylenediaminetetraacetic acid monovalent ion salt. Aqueous suspensions formulated with the composition are substantially colorless, allowing for broader application of aqueous suspensions containing guar gum.

Description

Technical Field

[0001] This invention belongs to the field of suspending agents, and more specifically, relates to a composition for improving the color of an aqueous suspension containing guar gum, its preparation method, and its uses. Background Technology

[0002] Suspension agents (also known as "thickeners") have a wide range of applications across various industries. Their functions include thickening, preventing sagging, suspending, adjusting the rheological properties of water, or a combination of these functions. Suspension agents are frequently used in many industries, including food processing, coatings, biotechnology, pharmaceuticals, and vaccines. Although used in small quantities, they can very effectively improve the viscosity of aqueous systems, increasing the texture and thickness of the system, or enhancing the suspension of solid materials in aqueous systems. While there is also a need for thickening in oil-phase systems, the use and demand for thickening in aqueous systems are the most significant.

[0003] Guar gum is a commonly used suspending agent, a free-flowing powder, generally white to light yellowish-brown, with excellent thickening properties. Structurally, guar gum is a nonionic neutral polysaccharide with a relative molecular weight of 200,000-300,000. It is soluble in both cold and hot water but not in organic solvents. The chemical composition of guar gum is galactomannan. Like most polymers with relatively high molecular weights, guar gum and its derivatives exhibit pseudoplastic rheological properties, although these properties are less pronounced than xanthan gum. In practical applications, these two suspending agents are often used in combination to help stabilize the dispersion of products such as dairy products.

[0004] Aqueous suspensions prepared with guar gum are generally yellowish-green, and this color becomes even more pronounced when guar gum is mixed with substances such as sodium polyacrylate. This can affect the use of the suspension in certain products; for example, when used in milky white material systems, the yellow tint can negatively impact the product's sensory appeal, limiting the application of guar gum. Therefore, eliminating the color of guar gum-containing aqueous suspensions would significantly expand the applicability of guar gum. Summary of the Invention

[0005] In one aspect, the present invention provides a composition for improving the color of an aqueous suspension containing guar gum, the composition comprising guar gum and a monovalent ion salt of ethylenediaminetetraacetic acid (EDTA).

[0006] In some preferred embodiments of the present invention, the weight ratio of guar gum to ethylenediaminetetraacetic acid monovalent ion salt is 1:0.1 to 10. In some more preferred embodiments of the present invention, the weight ratio of guar gum to ethylenediaminetetraacetic acid monovalent ion salt is 1:0.2 to 5, and even more preferably 1:0.5 to 2.

[0007] In some preferred embodiments of the present invention, the monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

[0008] In some preferred embodiments of the present invention, the composition further comprises additional suspending agents, dispersants, water quality conditioners, or combinations thereof.

[0009] In some preferred embodiments of the present invention, the additional suspending agent is selected from xanthan gum, sodium alginate, potassium alginate, ammonium alginate, carrageenan, pectin, guar gum, fenugreek gum, papaya seed gum, plantain seed gum, Artemisia argyi gum, gum arabic, gum arabic, dextran, veslaw gum, sodium carboxymethyl cellulose, ammonium carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, soluble starch, pregelatinized starch, carboxymethyl starch, polyethylene oxide, sodium polyacrylate, or combinations thereof.

[0010] In some preferred embodiments of the present invention, the dispersant is selected from glucose, sodium sulfate, potassium sulfate, lithium sulfate, ammonium sulfate, sodium chloride, potassium chloride, lithium chloride, ammonium chloride, or a combination thereof.

[0011] In some preferred embodiments of the present invention, the water quality conditioner is selected from monovalent polyphosphate salts. In some preferred embodiments of the present invention, the monovalent polyphosphate salt is selected from sodium polyphosphate, potassium polyphosphate, lithium polyphosphate, ammonium polyphosphate, or combinations thereof.

[0012] In some preferred embodiments of the present invention, the composition for improving the color of an aqueous suspension containing guar gum comprises the following components:

[0013] Guar gum, xanthan gum and / or sodium alginate and / or sodium polyacrylate, monovalent salts of ethylenediaminetetraacetic acid and monovalent salts of polyphosphate.

[0014] In some preferred embodiments of the present invention, the composition comprises the following components in weight percentages:

[0015]

[0016] In some preferred embodiments of the present invention, the composition comprises the following components in weight percentages:

[0017]

[0018] Glucose and / or sodium sulfate and / or potassium sulfate and / or ammonium sulfate and / or potassium chloride and / or sodium chloride and / or ammonium chloride balance.

[0019] Another aspect of the present invention provides a method for preparing the above-mentioned composition for improving the color of an aqueous suspension containing guar gum, the method comprising mixing the various components of the composition uniformly according to a conventional powder mixing method (e.g., mixing with a two-dimensional mixer or high-speed stirring).

[0020] In some preferred embodiments of the present invention, the composition of the present invention is packaged in a bottle sealed with a film, for example, with the bottle opening sealed with an aluminum film.

[0021] In another aspect, the present invention provides the use of compositions for improving the color of aqueous suspensions containing guar gum in the preparation of suspensions for use in suspended coccidia vaccines, pharmaceuticals, and additives.

[0022] In some preferred embodiments of the present invention, the coccidia vaccine is selected from coccidia oocyst vaccines.

[0023] In some preferred embodiments of the present invention, the coccidia oocyst vaccine is selected from chicken coccidia oocyst vaccine, rabbit coccidia oocyst vaccine, pigeon coccidia oocyst vaccine, duck coccidia oocyst vaccine, pig coccidia oocyst vaccine, bovine coccidia oocyst vaccine, and canine coccidia oocyst vaccine.

[0024] In another aspect, the present invention provides a method for preparing a suspension for use in suspended coccidia vaccines, drugs, or additives using the above-described composition for improving the color of guar gum-containing aqueous suspensions, the method comprising the following steps:

[0025] (1) The above composition for improving the color of the aqueous suspension containing guar gum is uniformly added to water and stirred until the guar gum is completely hydrated to obtain a colorless or essentially colorless aqueous suspension.

[0026] (2) Add the coccidiosis vaccine, drug or additive to the water.

[0027] When the composition of the present invention is added to water, the rate at which the suspending agent is added to the water is preferably controlled by a porous plate or a porous membrane.

[0028] Adding 1-100g of the composition of the present invention to 1L of water, for example, 3-100g or 3-50g, can prepare an aqueous medium with a viscosity of 20mPa·s to 2000mPa·s (data measured at 6rpm using a rotational viscometer), for example, a viscosity of 20mPa·s to 500mPa·s. This viscosity meets the suspension requirements of conventional drugs, coccidial vaccines, and additives (e.g., feed). For example, for coccidial oocysts, an aqueous medium viscosity of 30mPa·s to 150mPa·s provides excellent suspension.

[0029] The aqueous suspension containing guar gum prepared using the composition of the present invention is substantially colorless or essentially colorless, and this aqueous suspension has a wide range of applications and is convenient for use in different occasions.

[0030] Another aspect of the present invention provides the use of ethylenediaminetetraacetic acid monovalent ion salts in the preparation of colorless or substantially colorless aqueous suspensions containing guar gum.

[0031] In some preferred embodiments of the present invention, the monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or mixture thereof of ethylenediaminetetraacetic acid. Attached Figure Description

[0032] Figure 1a The photo shows a suspension prepared using 500mL of tap water (hardness approximately 200), 2.5g of guar gum, 2g of sodium polyphosphate, and 2.75g of glucose. The suspension appears yellowish-green.

[0033] Figure 1b The photo shows a suspension prepared using 500mL of tap water (hardness approximately 200), 2.5g of guar gum, 2g of sodium polyphosphate, 2g of disodium EDTA, and 2.75g of glucose. The suspension is essentially colorless.

[0034] Figure 1c The photo shows a suspension prepared using 500mL of water, 2.5g of guar gum, 2g of disodium EDTA, and 2.75g of glucose. The suspension is essentially colorless.

[0035] Figure 2a The photo shows a suspension prepared using 500mL of tap water (hardness approximately 200), 1.5g xanthan gum, 1.0g guar gum, 2g sodium polyphosphate, and 2.75g glucose. The suspension appears slightly yellowish-green.

[0036] Figure 2b The photo shows a suspension prepared using 500mL of tap water (hardness approximately 200), 1.5g xanthan gum, 1.0g guar gum, 2g sodium polyphosphate, 2g disodium EDTA, and 2.75g glucose. The suspension is essentially colorless.

[0037] Figure 3a The photo shows a suspension prepared using 500mL of tap water (hardness approximately 1000), 1.5g xanthan gum, 1.0g guar gum, 2g sodium polyphosphate, and 2.75g glucose. The suspension appears slightly yellowish-green.

[0038] Figure 3b The photo shows a suspension prepared using 500mL of tap water (hardness approximately 1000), 1.5g xanthan gum, 1.0g guar gum, 2g sodium polyphosphate, 2g disodium EDTA, and 2.75g glucose. The suspension is essentially colorless.

[0039] Figure 4aThe photo shows a suspension prepared using 500mL of tap water (hardness approximately 200), 2.5g of guar gum, 2g of sodium alginate, and 2.75g of glucose. The suspension appears slightly yellowish-green.

[0040] Figure 4b The photo shows a suspension prepared using 500mL of water, 2.5g of guar gum, 2g of sodium alginate, 2g of disodium EDTA, and 2.75g of glucose. The suspension is essentially colorless.

[0041] Figure 5a The photo shows a suspension prepared using 500mL of tap water (hardness approximately 200), 2g of sodium polyacrylate, and 3g of glucose. The suspension is almost colorless and transparent.

[0042] Figure 5b The photo shows a suspension prepared using 500mL of tap water (hardness approximately 200), 2.5g of guar gum, 2g of sodium polyacrylate, and 3g of glucose. The suspension is clearly yellow-green.

[0043] Figure 5c The photo shows a suspension prepared using 500mL of water, 2.5g of guar gum, 2g of sodium polyacrylate, 2g of disodium EDTA, and 3g of glucose. The suspension is essentially colorless. Detailed Implementation

[0044] In the process of researching the preparation of aqueous suspensions using suspending agents, the inventors of this application unexpectedly discovered, during experiments to reduce the influence of water hardness on suspending agents, that when disodium EDTA was added to an aqueous suspension prepared with guar gum, the yellow-green color of the guar gum-containing aqueous suspension disappeared, becoming colorless or essentially colorless. Subsequently, the inventors conducted further research and discovered that common monovalent EDTA ions can effectively improve the color of aqueous suspensions containing guar gum, and the addition of monovalent EDTA ions has little effect on the viscosity or suspension performance of the aqueous suspension containing guar gum. Based on this, the present invention was completed.

[0045] The mechanism by which the addition of a monovalent EDTA salt to an aqueous suspension containing guar gum causes the yellow-green color of the suspension to disappear is currently unclear. It is speculated that the monovalent EDTA ions interact with the chromophores in the guar gum structure, resulting in a lighter or absent color in the aqueous suspension. The yellow-green aqueous suspension formed by guar gum in water is not significantly affected by hard water, as it produces a yellow-green color in water of varying hardness. Adding a monovalent EDTA salt (such as disodium EDTA) can lighten or eliminate the color of the guar gum-containing aqueous suspension (here, "lighter or absent color," "colorless," and "white" are essentially the same in this invention, meaning the yellow-green color of the aqueous suspension lightens or disappears). Although EDTA monovalent salts such as disodium EDTA can be used as complexing agents in water treatment, not all water conditioners that can soften water have this effect. For example, polyphosphates (such as sodium polyphosphate), although similar to disodium EDTA in that they can complex with calcium and magnesium ions in hard water to soften the water, do not improve the color of water suspensions containing guar gum when added to them.

[0046] In the description of this invention, "multiple" refers to two or more.

[0047] In the description of this invention, "substantially colorless" means that the yellow-green color of the aqueous suspension containing guar gum becomes very light, close to colorless. Colorless here can also mean white, because the aqueous suspension formed after adding guar gum or a mixture containing guar gum to water does not become completely transparent, but the colloid appears white.

[0048] The monovalent ionic salts of ethylenediaminetetraacetic acid (EDTA) used in this invention include, but are not limited to, sodium, potassium, lithium, and ammonium salts of EDTA. In some preferred embodiments of this invention, the monovalent ionic salt of EDTA is selected from disodium EDTA.

[0049] When the mass ratio of guar gum to ethylenediaminetetraacetic acid monovalent salt in the composition of the present invention is 1:0.1 to 10, the color of the aqueous suspension containing guar gum can be improved. More preferably, the mass ratio of guar gum to ethylenediaminetetraacetic acid monovalent salt is 1:0.2 to 5, and even more preferably, it is 1:0.5 to 2.

[0050] The viscosity of aqueous suspensions prepared solely with guar gum sometimes fails to meet practical requirements. Therefore, it is typically used in combination with other suspending agents (also known as supplementary suspending agents). Thus, aqueous suspensions containing guar gum usually also contain supplementary suspending agents. When used together with guar gum, the supplementary suspending agent can have a coupling effect, improving the rheological properties of the aqueous suspension and achieving better suspension results with a smaller amount of suspending agent. Therefore, the composition of the present invention for improving the color of guar gum-containing aqueous suspensions may comprise a monovalent salt of ethylenediaminetetraacetic acid, guar gum, and a supplementary suspending agent.

[0051] Additional suspending agents that can be used in this invention include, but are not limited to, xanthan gum, sodium alginate, potassium alginate, ammonium alginate, carrageenan, pectin, guar gum, fenugreek gum, papaya seed gum, plantain seed gum, Artemisia argyi gum, gum arabic, gum arabic, dextran, veslaw gum, sodium carboxymethyl cellulose, ammonium carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, soluble starch, pregelatinized starch, carboxymethyl starch, and polyethylene oxide. Preferably, xanthan gum, sodium alginate, potassium alginate, ammonium alginate, polyethylene oxide, carboxymethyl cellulose, and sodium polyacrylate are preferred. More preferably, suspending agents that can themselves form light-colored aqueous suspensions are preferred, such as xanthan gum, guar gum, carboxymethyl cellulose, and polyethylene oxide and sodium polyacrylate.

[0052] Here, polyoxyethylene refers to ethylene oxide polymers with a large molecular weight, and the repeating segment is (CH2CH2O). The average molecular weight is between 88,000 and 880,000, which is the polyoxyethylene in the pharmacopoeia. The average molecular weight is obtained with reference to the method of polyethylene glycol 4000 in the veterinary pharmacopoeia.

[0053] Sodium polyacrylate is nearly colorless and transparent when it is hydrated on its own, but when combined with guar gum, its color becomes darker than that of guar gum itself. However, the addition of disodium EDTA can almost eliminate the darkening of the color.

[0054] Although dispersants have little or no effect on the viscosity and suspension properties of aqueous suspensions containing guar gum, they are necessary for compositions containing guar gum in powder form. They disperse and dilute the guar gum or its composition, allowing it to be more evenly distributed in water when added. This enables the suspending agent in the composition to be more easily and quickly fully hydrated when the aqueous suspension is prepared.

[0055] The excipients can be used to disperse the suspending agent, thus ensuring a more uniform dispersion of the suspending agent in water when it is added, preventing the formation of large lumps or clumps. Excipients that can be used in this invention include, but are not limited to, glucose, sucrose, sodium sulfate, potassium sulfate, ammonium sulfate, sodium chloride, potassium chloride, ammonium chloride, and soluble starch.

[0056] The composition for improving the color of guar gum-containing aqueous suspensions of the present invention is prepared by mixing the components in powder form using conventional methods (e.g., using a two-dimensional mixer or by using an auxiliary agent dilution method). To facilitate control over the rate at which the composition is added to water during use, the prepared powdered composition can be packaged in bottles, particularly in bottles sealed with a membrane (e.g., aluminum foil). During use, multiple small holes are punched in the sealing aluminum foil, allowing the powdered composition to be poured out of the bottle at a uniform rate and evenly sprinkled into the water, thus facilitating hydration.

[0057] Although disodium EDTA softens hard water and can reduce the impact of some suspending agents on water hardness, experiments have shown that its effectiveness in eliminating the effects of hard water is sometimes less than ideal. For example, the viscosity of sodium alginate in water is sensitive to water hardness; when sodium alginate is added to hard water, gelation (gel blocks) occurs, and the addition of disodium EDTA cannot completely eliminate this gelation. Therefore, to further reduce the impact of the system on hard water, the composition of the present invention preferably includes a water quality regulator that improves water quality. For example, monovalent polyphosphate salts are more effective than disodium EDTA in improving the content of calcium and magnesium ions in hard water, especially for aqueous suspensions containing sodium alginate, and can therefore be used as water quality regulators in the composition of the present invention. The term "monovalent polyphosphate salt" as used herein refers to a salt formed by polyphosphate ions and monovalent cations, including but not limited to sodium polyphosphate, potassium polyphosphate, lithium polyphosphate, and ammonium polyphosphate. The monovalent polyphosphate salts used in this invention, such as sodium polyphosphate, are generally dimeric or trimeric polyphosphates with different chain lengths, and are highly soluble in water. They have a strong ability to chelate metal ions, especially calcium and magnesium ions, and therefore can be used as preferred water conditioners (i.e., water softeners).

[0058] The aqueous suspensions prepared using the composition of the present invention for improving the color of guar gum-containing aqueous suspensions are essentially colorless (here, colorless refers more to a state similar to white). If necessary, dyes can be added to them for subsequent purposes, and their inherent colorlessness also facilitates subsequent color adjustment.

[0059] The present invention will be further illustrated below with reference to specific embodiments. These specific embodiments are implemented based on the technical solution of the present invention, and detailed implementation methods and operating procedures are provided. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Experimental methods in the following embodiments that do not specify specific conditions are generally carried out under conventional conditions. Unless otherwise stated, proportions and percentages are by weight.

[0060] Viscosity testing was conducted using a rotational viscometer [NDJ-1 type rotational viscometer, Shanghai Yueping Scientific Instruments Co., Ltd.] at various rotational speeds.

[0061] Example 1

[0062] The composition of the suspending agent composition, the hardness of the water used, and the color of the prepared suspension are shown in Table 1 below.

[0063] Table 1

[0064]

[0065]

[0066] As shown in the table above, adding disodium EDTA to an aqueous suspension containing guar gum produces a white aqueous suspension. Adding only sodium polyphosphate to an aqueous suspension containing guar gum produces a yellow-green aqueous suspension; when this suspension also contains additional suspending agents, the color is diluted and becomes lighter. The addition of disodium EDTA to the aqueous suspensions all resulted in a white color, indicating that disodium EDTA plays a crucial role in improving the color of aqueous suspensions containing guar gum. Sodium polyphosphate, however, does not have the effect of improving the yellow-green color of guar gum.

[0067] The viscosity data of the above suspensions are shown in Table 2.

[0068] Table 2. Viscosity and thixotropic coefficient of the prepared suspending agent (viscosity ratio at 6 rpm and 60 rpm)

[0069]

[0070] Among the suspensions with different compositions mentioned above, the viscosity of the suspension aid with both disodium EDTA and sodium polyphosphate is slightly lower than that with only sodium polyphosphate, but the difference is not significant, especially for suspensions prepared with low-hardness water; the difference is also minimal for suspensions prepared with high-hardness water (1000 ppm). Therefore, while adding disodium EDTA improves the color of the suspension aid, its effect on viscosity is not substantial.

[0071] Example 2

[0072] Aqueous suspensions were prepared using a composition containing guar gum and sodium alginate, wherein the sodium alginate content was 1.5 g / 500 mL and the sodium polyphosphate content was 2 g / 500 mL (the amount of sodium polyphosphate added was based on previous experimental results; adding 2 g of sodium polyphosphate to 500 mL of 800 ppm hard water can eliminate the effect of water hardness on sodium polyacrylate). Aqueous suspensions were prepared according to different amounts of guar gum and disodium EDTA, and the specific amounts are shown in Table 3 below.

[0073] Table 3. Raw materials and their dosage, water hardness and color of the prepared aqueous suspension

[0074]

[0075] As shown in the table, aqueous suspensions obtained using sodium alginate alone are white or nearly colorless and transparent, regardless of the addition of disodium EDTA and / or sodium polyphosphate. For systems containing guar gum and sodium alginate, the system without disodium EDTA is yellowish-green. Adding disodium EDTA to the aqueous suspension lightens and eventually disappears the original yellowish-green color, resulting in a white appearance; however, adding sodium polyphosphate alone does not change the color. Suspensions prepared with water of low hardness containing guar gum are yellowish-green, but adding disodium EDTA turns the system white. Therefore, regardless of whether the suspension is prepared with water of high or low hardness, adding disodium EDTA improves the color of the guar gum-containing aqueous suspension, causing the original yellowish-green color to disappear and turn white (colorless). The viscosity data for the above suspensions are shown in Table 4.

[0076] Table 4. Viscosity of the prepared suspending agent

[0077]

[0078]

[0079] Sodium alginate is sensitive to water hardness. When added to hard water, it forms gel clumps (No. 6), resulting in lower viscosity, but the solution itself is white (or colorless). Using disodium EDTA alone (No. 3) does not effectively mitigate the effect of sodium alginate on water hardness, resulting in severe gel formation and a slightly higher viscosity than the suspension in No. 6. However, adding sodium polyphosphate eliminates the effect of water hardness on sodium alginate, the gel clumps disappear, and the viscosity increases (No. 1). Guar gum itself has low viscosity, but adding guar gum to an aqueous suspension containing sodium alginate (No. 2) increases the overall viscosity, turning the system yellowish-green. Adding both disodium EDTA and sodium polyphosphate lightens the color of the system, and slightly decreases the viscosity (No. 5). Even without disodium EDTA and sodium polyphosphate, the viscosity of the aqueous suspension prepared using low-hardness water is relatively high, but it is yellowish-green.

[0080] The comparison between suspensions numbered 1 and 4 shows that the viscosity of the suspension with both disodium EDTA and sodium polyphosphate added is slightly higher than that of the suspension with only sodium polyphosphate added, but the difference is not significant.

[0081] For suspensions prepared using water with low hardness, adding disodium EDTA improves the color but does not significantly reduce the viscosity.

[0082] The above experiments show that when disodium EDTA is added to an aqueous suspension containing guar gum, the yellow-green color of the suspension disappears, while it remains yellow-green when not added. Furthermore, the addition of disodium EDTA has little effect on the viscosity of the aqueous suspension. Therefore, for systems containing guar gum, adding disodium EDTA to improve the color has minimal impact on its viscosity characteristics.

[0083] Example 3

[0084] The suspending agent mixture was prepared by mixing sodium alginate, guar gum, sodium polyphosphate, disodium EDTA, and glucose in the following proportions: 1.5g sodium alginate, 1.5g guar gum, 4g sodium polyphosphate, 3g disodium EDTA, and 10g glucose.

[0085] 20g of the solution was added to 1L of tap water with a hardness of approximately 200ppm to obtain a nearly colorless aqueous suspension. The viscosity was tested, and the results are shown in Table 5.

[0086] Table 5

[0087] engine speed / rpm 6 12 30 60 Viscosity / mPa.s 55 50 45 37.5

[0088] The suspension solution was used for the chicken coccidiosis oocyst suspension experiment. A quadrivalent live chicken coccidiosis vaccine, batch number: 21080401, was used. The specific process for preparing the coccidiosis oocyst suspension is as follows:

[0089] Add 30.9 mL of vaccine (equivalent to 6180 doses) to 2 L of the above suspension and stir thoroughly. Timing begins from the point of thorough mixing, and samples are taken at 0–0.5 h, 2 h, 4 h, and 5.5 h–6 h. Sampling sites are located at the upper liquid surface (labeled upper layer), the 1200 mL mark of the 2 L beaker (labeled middle layer), and the 400 mL mark of the 2 L beaker (labeled lower layer). Four samples are taken from each liquid surface, with 1 mL taken from each point, and the mixture is used as the sample for that liquid surface. Take 10 μL from each of these samples and place it on a glass slide. Count the number of oocysts under a microscope at 10 × 10 magnification. The results are shown in Table 6 below.

[0090] Table 6. Oocyte Count Results (Unit: Number of Oocytes)

[0091]

[0092]

[0093] The results showed that the suspension prepared using the above-mentioned suspending agent composition had good stability and could meet the requirements for use of suspended chicken coccidiosis vaccine within 6 hours.

[0094] Example 4

[0095] A suspending agent composition was obtained by mixing 1g sodium alginate, 0.5g xanthan gum, 1.5g guar gum, 4g sodium polyphosphate, 3g disodium EDTA, 2g sodium sulfate, and 18g glucose.

[0096] Adding 30g of the suspending agent composition to 1L of tap water with a hardness of approximately 800ppm yielded a virtually colorless suspension, indicating that adding disodium EDTA to guar gum can produce a virtually colorless solution.

[0097] The suspension prepared using this suspending agent composition has good stability and can meet the requirements for use of suspended chicken coccidiosis vaccine within 6 hours.

[0098] Example 5

[0099] Sodium polyacrylate, guar gum, sodium polyphosphate, disodium EDTA, and glucose were mixed in different proportions to obtain a suspending agent composition. The suspending agent composition was added to tap water with a hardness of 200, and the color of the resulting aqueous suspension is shown in Table 7 below.

[0100] Table 7. Raw materials, water hardness, and color of the prepared aqueous suspension

[0101]

[0102] As can be seen from the table above and the colors of the obtained aqueous suspensions, the yellow-green guar gum suspension darkened further after the addition of sodium polyacrylate. Sodium polyacrylate, upon hydration in water, forms an almost colorless and transparent suspension. Adding disodium EDTA to the suspension containing guar gum and sodium polyacrylate also resulted in a near-colorless (white) suspension, indicating that the monovalent salt of EDTA has a significant effect on improving the color of guar gum-containing colloids.

[0103] The above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of the invention. Various variations can be made to the above embodiments of the present invention. That is, all simple and equivalent changes and modifications made based on the claims and description of this application fall within the protection scope of the claims of this patent. All aspects not described in detail in this invention are conventional technical content.

Claims

1. A composition for improving the color of an aqueous suspension containing guar gum, characterized in that, The composition consists of guar gum, a monovalent ion salt of ethylenediaminetetraacetic acid, and glucose. The weight ratio of guar gum to the monovalent ion salt of ethylenediaminetetraacetic acid is 1:0.5~2. The monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

2. A composition for improving the color of an aqueous suspension containing guar gum, characterized in that, The composition comprises guar gum, xanthan gum, a monovalent ion salt of ethylenediaminetetraacetic acid, glucose, and sodium polyphosphate. The weight ratio of guar gum to the monovalent ion salt of ethylenediaminetetraacetic acid is 1:0.5~2. The monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

3. A composition for improving the color of an aqueous suspension containing guar gum, characterized in that, The composition consists of guar gum, a monovalent ion salt of ethylenediaminetetraacetic acid, glucose, and sodium polyphosphate. The weight ratio of guar gum to the monovalent ion salt of ethylenediaminetetraacetic acid is 1:0.5~2. The monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

4. A composition for improving the color of an aqueous suspension containing guar gum, characterized in that, The composition comprises guar gum, a monovalent ion salt of ethylenediaminetetraacetic acid, glucose, sodium alginate, and sodium polyphosphate. The weight ratio of guar gum to the monovalent ion salt of ethylenediaminetetraacetic acid is 1:0.5~2. The monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

5. A composition for improving the color of an aqueous suspension containing guar gum, characterized in that, The composition consists of guar gum, a monovalent ion salt of ethylenediaminetetraacetic acid, glucose, and sodium alginate. The weight ratio of guar gum to the monovalent ion salt of ethylenediaminetetraacetic acid is 1:0.5~2. The monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

6. A composition for improving the color of an aqueous suspension containing guar gum, characterized in that, The composition comprises guar gum, xanthan gum, a monovalent ion salt of ethylenediaminetetraacetic acid, glucose, sodium alginate, sodium polyphosphate, and sodium sulfate. The weight ratio of guar gum to the monovalent ion salt of ethylenediaminetetraacetic acid is 1:0.5~2. The monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

7. A composition for improving the color of an aqueous suspension containing guar gum, characterized in that, The composition consists of guar gum, a monovalent ion salt of ethylenediaminetetraacetic acid, glucose, and sodium polyacrylate. The weight ratio of guar gum to the monovalent ion salt of ethylenediaminetetraacetic acid is 1:0.5~2. The monovalent ionic salt of ethylenediaminetetraacetic acid is selected from the sodium salt, potassium salt, lithium salt, ammonium salt, or combination thereof of ethylenediaminetetraacetic acid.

8. Use of the composition for improving the color of an aqueous suspension containing guar gum according to any one of claims 1 to 7 in the preparation of a suspension for a suspended coccidioidomyces vaccine.

9. The use according to claim 8, characterized in that, The coccidiosis vaccine is selected from coccidiosis oocyst vaccines.

10. The use according to claim 9, characterized in that, The coccidia oocyst vaccine is selected from chicken coccidia oocyst vaccine, rabbit coccidia oocyst vaccine, pigeon coccidia oocyst vaccine, duck coccidia oocyst vaccine, swine coccidia oocyst vaccine, bovine coccidia oocyst vaccine, and canine coccidia oocyst vaccine.

11. A method for preparing a suspension for a suspended coccidioidomyces vaccine using the composition for improving the color of an aqueous suspension containing guar gum according to any one of claims 1 to 7, characterized in that, The method includes the following steps: (1) The composition for improving the color of an aqueous suspension containing guar gum according to any one of claims 1 to 7 is uniformly added to water while stirring until the guar gum is completely hydrated to obtain a colorless aqueous suspension; (2) Add the coccidiosis vaccine to the aqueous suspension.

12. Use of the composition for improving the color of an aqueous suspension containing guar gum according to any one of claims 1 to 7 in the preparation of a colorless aqueous suspension containing guar gum.

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