A nanoenzyme immunoassay system

Abstract

The present invention relates to the technical field of nanoenzyme immunoassay equipment, and specifically to a nanoenzyme immunoassay system, comprising a frame and a controller, wherein the frame is provided with a reagent card transport device electrically connected to the controller, a sample automatic sampling device, a reagent card installation and detection device, and a sample staining automatic pipetting device; the reagent card installation and detection device comprises a turntable and a turntable drive assembly; the turntable is provided with a number of reagent card slots evenly distributed around the center of a circle; the reagent card transport device comprises a first guide rail mounted on the frame, a magazine sliding on the first guide rail, an X-axis drive unit driving the magazine horizontal reciprocating motion, and a reagent card feeding assembly; the reagent card feeding assembly comprises a first slider, a first slider drive unit driving the first slider to reciprocate motion, and a card pushing unit fixed on the slider. The present invention can achieve highly automated detection, increase the detection volume, liberate manpower, and solve the problem of low detection efficiency of existing equipment.

Description

Technical Field

[0001] The present invention relates to the technical field of nanoenzyme immunoassay equipment, and in particular to a nanoenzyme immunoassay system. Background Art

[0002] The nanoenzyme immunoassay analyzer is a medical testing device developed based on enzyme-linked immunosorbent assay (ELISA) technology and is one of the commonly used devices for medical testing. Currently, nanoenzyme immunoassay analyzers often use a single-channel injection system. When in use, a reagent card containing a sample is inserted into the sample inlet of the nanoenzyme immunoassay analyzer. A color-developing solution is added to the reagent card via a liquid-adding mechanism, and then the excess color-developing solution in the reagent card is aspirated via a liquid-sucking mechanism. After the intensity of the color developed by the reagent card is detected using a detection head, the reagent card is removed and the next reagent card containing a sample is inserted for testing. This operation is relatively cumbersome, with poor operational continuity and low detection efficiency. Summary of the Invention

[0003] The purpose of the present invention is to provide a nanoenzyme immunoassay system to achieve highly automated detection, increase the detection volume, liberate manpower, and solve the problem of low detection efficiency of existing equipment.

[0004] In order to achieve the above object, a nanoenzyme immunoassay system is provided, comprising a frame and a controller, wherein the frame is provided with a reagent card transport device electrically connected to the controller, a sample automatic sampling device, a reagent card installation and detection device, and a sample staining automatic pipetting device;

[0005] The reagent card installation and detection device includes a turntable and a turntable drive assembly for driving the turntable; the turntable is provided with a plurality of reagent card slots evenly distributed around the center of the circle;

[0006] The reagent card transport device includes a first guide rail mounted on a frame, a magazine sliding on the first guide rail, an X-axis drive unit for driving the magazine to move horizontally back and forth, and a reagent card feeding assembly; the magazine includes a plurality of interlayer cavities that evenly divide its internal space, and a reagent card magazine mounted in the interlayer cavities, wherein a plurality of reagent cards are stacked up and down in the reagent card magazine; the bottom surfaces of the reagent card magazine and the interlayer cavities are both provided with through grooves communicating with the interior, and the through grooves extend through the front and rear sides of the reagent card magazine and the interlayer cavities; a first opening located on the front side of the reagent card magazine and the interlayer cavity is for allowing the reagent card to pass through and can be opposite to a notch of a reagent card slot on the turntable, and the width of the through groove is smaller than the width of the reagent card;

[0007] The reagent card feeding assembly includes a first slider, a first slider driving unit that drives the first slider to reciprocate, and a card pushing unit fixed to the slider. The card pushing unit includes a fixed bracket and a rotating block hinged to the fixed bracket and rotating in the vertical direction. The basic state of the rotating block is a vertical state and the upper end can slide in the through groove and limit the upper end of the rotating block from flipping backward.

[0008] Furthermore, a limiting post is provided in the center of the central area of the turntable and at the center of the end position of the reagent card slot, and a limiting buckle is provided at the front end of the reagent card, and the limiting post is matched with the limiting buckle for engagement.

[0009] Furthermore, a QR code is provided on the lower surface of the reagent card, and a scanner for scanning the QR code is provided on the rack, and the scanner is electrically connected to the controller.

[0010] Furthermore, the reagent card installation detection device further includes a reagent card data acquisition component for acquiring data from the reagent card on the turntable and a reagent card ejection component for ejecting the reagent card from the reagent card slot.

[0011] Furthermore, the reagent card ejection component includes a first reagent card ejection component for ejecting a reagent card that fails scanning and identification from the reagent card slot and a second reagent card ejection component for ejecting a reagent card that completes data collection from the reagent card slot.

[0012] Furthermore, the first reagent card ejection assembly and the second reagent card ejection assembly have the same structure and are both arranged above the turntable, including: a structural bracket fixed on the frame, on which a second slider is provided, a second slider driving unit that drives the first slider to reciprocate, and a bar-shaped lever fixed to the lower end of the second slider and arranged horizontally, the end of the bar-shaped lever is provided with a card ejection paddle, and the card ejection paddle is abutted against the end of the reagent card on the turntable and close to the center end of the turntable.

[0013] Furthermore, a recovery bin for recovering reagent cards that have failed code scanning and have been returned by the first reagent card return assembly is detachably mounted on the side of the magazine compartment.

[0014] Furthermore, the rack is provided with a waste card collection box for recovering reagent cards that have completed data collection, and a waste card collection box driving unit for driving the waste card collection box to move back and forth, and the waste card collection box driving unit is electrically connected to the controller.

[0015] Furthermore, the automatic sample sampling device includes an XZ dual-axis manipulator arranged above the frame, a sample box arranged at the bottom of the frame, a sample box driving unit that drives the sample box to reciprocate in the Y-axis direction, and a clamping and fixing unit that clamps and fixes the sample taken out of the sample box by the XZ dual-axis manipulator.

[0016] Furthermore, the sample staining automatic pipetting device includes an XYZ three-axis manipulator arranged above the frame, a tip head collecting box arranged at the bottom of the frame, and a tip head collecting box driving unit that drives the tip head collecting box to reciprocate in the Y-axis direction. The tip head collecting box includes a new tip head installation box and a waste tip head recovery box that are separately arranged. The new tip head installation box and the waste tip head recovery box are both provided with a number of placement slots for placing tip heads.

[0017] Principles and advantages:

[0018] 1. The automatic sample sampling device and the automatic sample staining pipetting device can automatically complete the sample sampling operation (the XZ two-axis manipulator is used to remove a sample tube, open the lid, and clamp the sample tube). The automatic sample staining pipetting device can automatically remove the sample test solution from the opened sample tube through the XYZ three-axis manipulator. Then, in conjunction with the reagent card installation detection device, several reagent cards are installed in the reagent card slot on the turntable to automatically pipette the sample test solution into the reagent card, so that the reagent card data acquisition component can perform subsequent automatic data acquisition. The entire process is highly automated, which greatly saves labor costs and has high detection efficiency.

[0019] 2. The magazine compartment includes a plurality of interlayer cavities that evenly divide its internal space and a reagent card magazine installed in the interlayer cavities. Therefore, multiple reagent cards can be loaded at one time, which is convenient for continuous automatic detection of batch samples, thereby improving work efficiency. The first slider driving unit of the reagent card feeding assembly can drive the first slider to reciprocate, so that the rotating block, whose basic state is vertical and whose upper end can slide in the through groove, will automatically push the reagent card at the bottom of the reagent card magazine into the reagent card slot of the turntable. The controller then controls the turntable driving assembly to rotate the turntable to expose and align the next empty reagent card slot. The first slider driving unit of the reagent card feeding assembly is then repeatedly driven to realize continuous filling of reagent cards. This is so as to cooperate with the sample automatic sampling device and the sample staining automatic liquid transfer device to complete the detection and analysis continuously and highly automatically.

[0020] 3. The base state of the rotating block is vertical. In other words, the mass of the upper end of the rotating block is less than that of the lower end, similar to the principle of a tumbler, which facilitates automatic resetting to the initial state. Since the upper end of the rotating block is restricted from flipping backward, the rotating block does not deflect when pushing the reagent card forward, thus achieving a position limit. This also facilitates the upper end of the rotating block to press against the tail of the reagent card and continue to push forward, allowing the reagent card to escape from the first opening and enter the reagent card slot of the turntable, making card pushing easier and more efficient. When the rotating block resets, the upper end of the rotating block will contact the front end of the reagent card. Since the upper end of the rotating block can flip forward but only cannot flip backward, the rotating block will tilt at a certain angle and will not have any pushing effect on the reagent card. It will then return to its initial position, ready to push the next reagent card. At the same time, with the X-axis drive unit and guide rails, automatic card replenishment can be achieved. The entire process is highly automated, greatly saving labor costs and improving detection efficiency.

[0021] 4. The first reagent card ejector removes unsuccessful reagent cards from the reagent card slot, preventing accidental mixing of reagent cards and project-to-reagent mismatches. The second reagent card ejector removes completed data collection cards from the reagent card slot to the waste card collection bin, ensuring continuous, automated operation of the entire system. This significantly reduces labor costs and improves testing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is an isometric view of the overall structure of a nanoenzyme immunoassay system according to an embodiment of the present invention;

[0023] Figure 2 This is an isometric view of the combination of the reagent card feeding assembly, the first reagent card ejection assembly, the second reagent card ejection assembly, the optical path detection module, and the turntable;

[0024] Figure 3 for Figure 1 Axonometric view after removing the reagent card transport device;

[0025] Figure 4 It is a partial cross-sectional view of the optical path detection module;

[0026] Figure 5 for Figure 1 An enlarged schematic diagram of the location of the rotating block of the middle card push unit;

[0027] Figure 6 This is an axonometric view of the reagent card. DETAILED DESCRIPTION

[0028] The following is further described in detail through specific implementation methods:

[0029] The reference numerals in the drawings of the specification include: base plate 1, cubic frame 2, waste card collection box 3, tip head collection box 4, barcode scanner 5, fixed bracket 6, rotating block 7, recovery bin 8, sample box 9, magazine compartment 10, interlayer cavity 11, reagent card magazine 12, XZ two-axis manipulator 13, turntable 14, first reagent card ejection assembly 15, limiting column 16, reagent card slot 17, XYZ three-axis manipulator 18, second reagent card ejection assembly 19, bar lever 20, ejection paddle 21, optical path detection module 22, clamping and fixing unit 23, light emitting unit 24, lens 25, light sensor 26, limiting buckle 27, detection window 28, sample loading window 29, dilution cup 30.

[0030] Example

[0031] A nanoenzyme immunoassay system, basically Figure 1-Figure 5 As shown, the device comprises a frame and a controller. The frame comprises a base plate 1 and a cubic frame 2 fixed to the upper surface of the base plate. Made of POM, the entire structure is lightweight, and the split-body design reduces the weight of the entire mounting body to a certain extent. The controller uses a conventional industrial PLC. The frame is equipped with a reagent card transport device electrically connected to the controller, an automatic sample sampling device, a reagent card installation and detection device, and an automatic sample staining and liquid transfer device.

[0032] like Figure 2 As shown, the reagent card installation detection device includes a horizontally rotating turntable 14 and a turntable 14 drive assembly that drives the turntable 14; the turntable 14 is provided with a number of reagent card slots 17 evenly distributed around the center of the circle, and the turntable 14 is provided with an electric heating belt electrically connected to the controller (the electric heating belt can incubate the reagent card to which the test solution sample is added). The center area of the turntable 14 and the center of the end position of the reagent card slot 17 are provided with a limiting column 16; the turntable 14 drive assembly is fixed to the center position of the base plate 1, and the turntable 14 drive assembly is a stepping motor or a servo motor and is arranged at the bottom of the turntable 14, and is electrically connected to the controller. In this embodiment, a high-cost but high-precision servo motor is selected. The reagent card slots 17 on the turntable 14 are set to sixteen.

[0033] like Figure 1 As shown, the reagent card transport device includes a first guide rail mounted on a frame, a cartridge magazine 10 sliding on the first guide rail, an X-axis drive unit that drives the cartridge magazine 10 to reciprocate horizontally, and a reagent card feeding assembly. In the present embodiment, the first guide rail is mounted on the upper end of the front side of the cubic frame 2 and adopts a dual-guide rail structure. The upper end of the cartridge magazine 10 is provided with four sliding blocks, which cooperate with the dual-guide rail structure to make the entire module motion high and stable, and the entire module hardly shakes. The X-axis drive unit is a combination of a conventional screw rod and a stepper motor, with high control accuracy, and is also electrically connected to the controller.

[0034] like Figure 1 As shown, the magazine compartment 10 includes a plurality of interlayer cavities 11 that evenly divide its internal space, and a reagent card magazine 12 mounted within each of the interlayer cavities 11. The reagent card magazine 12 contains a plurality of reagent cards stacked one above the other. In this embodiment, the magazine compartment 10 has six interlayer cavities 11, each of which can accommodate a reagent card magazine 12. The reagent card magazine 12 can stack sixteen reagent cards one above the other. The internal space of the reagent card magazine 12 matches the shape of the reagent cards, but with a certain gap to facilitate sliding.

[0035] like Figure 6 As shown, the reagent card is an elongated body with a limit buckle 27 at the front end, which engages with the limit post 16 on the turntable 14. The upper surface of the elongated body is provided with a detection window 28, a sample loading window 29, and two dilution cups 30, and the bottom surface is provided with a QR code. The detection window 28 is used to view test results or collect test results through visual data acquisition equipment, while the two dilution cups 30 are used by the sample staining automatic pipetting device to dilute the sample solution collected from the sample tube. The sample loading window 29 is used by the sample staining automatic pipetting device to load the diluted sample solution.

[0036] like Figure 1 As shown, the bottom surfaces of the reagent card clip 12 and the interlayer cavity 11 are both provided with through grooves communicating with the interior, and the through grooves penetrate the front and rear sides of the reagent card clip 12 and the interlayer cavity 11 (in this embodiment, in order to facilitate understanding of the interior of the clip compartment 10, the plate corresponding to the rear side is not drawn or can be cancelled in actual application); the first opening located on the front side of the reagent card clip 12 and the interlayer cavity 11 is for the reagent card to pass through and can be opposite to the notch of a reagent card slot 17 on the turntable 14; the second opening located on the rear side of the reagent card clip 12 and the interlayer cavity 11 cannot be used for the reagent card to pass through; the width of the through groove is smaller than the width of the reagent card.

[0037] like Figure 2 As shown, the reagent card feeding assembly includes a first slider, a first slider driving unit that drives the first slider to reciprocate, and a card pushing unit fixed to the slider. In this embodiment, the first slider driving unit is also a combination of a conventional screw and a stepping motor, with high control accuracy, and is also electrically connected to the controller. The screw is parallel to the Y-axis, and both ends are provided with a fixed seat fixed to the base plate 1. One fixed seat is arranged below the turntable 14 and at the front end of the upper surface of the base plate 1 (scanning upward), and the other fixed seat is arranged at the front end of the base plate 1; a guide rail is provided between the two fixed seats, and a code scanner 5 (horizontal scanning) for scanning the two-dimensional code on the bottom surface of the reagent card is slidably connected to the guide rail. The code scanner 5 is electrically connected to the controller.

[0038] like Figure 2 、 Figure 5 As shown, the card pushing unit includes a fixed bracket 6 and a rotating block 7 hingedly connected to the fixed bracket 6 and capable of vertical rotation. A spherical counterweight is positioned at the bottom of the rotating block 7. The rotating block 7 is in a vertical position in its basic state, and its upper end can slide within a through slot, restricting the upper end of the rotating block 7 from tilting backward. In this embodiment, the fixed bracket 6 is L-shaped and comprises a first vertical portion and a second vertical portion. The first vertical portion is parallel to the Y-axis, with its free end connected to the first slider. The second vertical portion is parallel to the X-axis, with its free end hinged to the rotating block 7 capable of vertical rotation. The structural principle is simplified: similar to a baffle positioned at 270° in rectangular coordinates, the hinge point is to the right of the zero point, and the rotating block 7 is positioned with a light weight on the top and a heavy weight on the bottom. Therefore, the rotating block 7 can only rotate counterclockwise (i.e., forward rotation in this embodiment, pointing forward toward the face), but cannot rotate clockwise (i.e., backward rotation in this embodiment). The rotating block 7, the two fixed brackets, and the guide rails provided between the two fixed brackets are located in the same numerical plane. The first slider, the first slider driving unit driving the first slider to reciprocate, and the blocking code scanner 5 are staggered so that both can work normally without interfering with each other.

[0039] like Figure 2 、 Figure 4 As shown, the reagent card installation and detection device further includes a reagent card data acquisition component for acquiring data from the reagent card on the turntable 14 and a reagent card ejection component for ejecting the reagent card from the reagent card slot 17 .

[0040] The reagent card ejection assembly includes a first reagent card ejection assembly 15 for ejecting a reagent card that fails scanning and identification from the reagent card slot 17 and a second reagent card ejection assembly 19 for ejecting a reagent card that has completed data collection from the reagent card slot 17 .

[0041] The first reagent card ejection assembly 15 and the second reagent card ejection assembly 19 have the same structure and are both fixed to the cubic frame 2 and located above the turntable 14. The first reagent card ejection assembly 15 and the second reagent card ejection assembly 19 are arranged at 90 degrees in their longitudinal direction.

[0042] like Figure 2 As shown, the first reagent card ejection assembly 15 comprises a structural support fixed to the frame, on which is mounted a second slider, a second slider drive unit that drives the first slider in reciprocating motion, and a card ejection paddle 21 fixed to the lower end of the second slider. The card ejection paddle 21 abuts against the end of the reagent card on the turntable 14, near the center end. The second slider drive unit is also a conventional lead screw and stepper motor combination, with the lead screw parallel to the Y-axis, providing high control precision, and is also electrically connected to the controller.

[0043] like Figure 1As shown, a recovery bin 8 for recovering reagent cards that have failed to pass the code scanning and have been ejected by the first reagent card ejection assembly 15 is detachably mounted on the left side of the magazine compartment 10. In this embodiment, the recovery bin 8 is fixed by bolts to achieve detachable connection.

[0044] like Figure 2 As shown, the second reagent card ejection assembly 19 includes: a structural support fixed to the frame and an optical path detection module 22 located on the side of the middle portion of the structural support. The optical path detection module 22 collects test data from the reagent card. To ensure that the optical path detection module 22 does not interfere with the normal operation of the optical path detection module 22, the structure of the second reagent card ejection assembly 19 has been slightly adjusted. The structural support is equipped with a second slider, a second slider drive unit that drives the first slider to reciprocate, and a bar-shaped lever 20 fixed to the lower end of the second slider and arranged laterally (the same function as the L-shaped fixed bracket 6 described above, to avoid interference with other equipment along the same path). The end of the bar-shaped lever 20 is equipped with a card ejection paddle 21, which abuts against the end of the reagent card on the turntable 14 and near the center end of the turntable 14. The second slider drive unit is also a conventional combination of a lead screw and a stepper motor, but the lead screw is parallel to the X-axis, which provides high control precision and is also electrically connected to the controller.

[0045] like Figure 4 As shown, the light path detection module 22 includes a housing, a cross-shaped channel is provided in the housing, the cross-shaped channel includes a transverse channel and a vertical channel, a 135° lens 25 is provided at the intersection of the transverse channel and the vertical channel, a light emitting unit 24 for irradiating light downward is provided at the top of the vertical channel, and a light sensor 26 for receiving and analyzing light is provided on the left side of the transverse channel. The light emitting unit 24 and the light sensor 26 are both electrically connected to the controller. The principle is: there is a color-developing component in the reagent card, and if the sample test solution has the component to be detected, a color-developing reaction will be generated. At this time, the light emitting unit 24 will irradiate the color-developing area, and the color of the color-developing reaction will be collected and analyzed by the reflection of light through the 135° lens 25.

[0046] like Figure 1 、 Figure 3 As shown, the rightmost side of the chassis' baseplate 1 houses a waste card collection bin 3 for recovering reagent cards that have completed data collection, as well as a waste card collection bin 3 drive unit that drives the waste card collection bin 3 back and forth. The drive unit is electrically connected to a controller. The waste card collection bin 3 drive unit is also a conventional lead screw and stepper motor combination, with the lead screw parallel to the Y-axis. Combined with a guide rail at the bottom of the waste card collection bin 3, this enables reciprocating movement of the waste card collection bin 3 along the Y-axis, preventing discharged reagent cards from accumulating in the same location in the waste card collection bin 3. This reciprocating movement ensures a more even distribution of discharged reagent cards, reducing the need for manual replacement of the waste card collection bin 3.

[0047] like Figure 1 、 Figure 3 As shown, the automatic sample sampling device includes an XZ dual-axis manipulator 13 disposed above the frame, a sample box 9 disposed at the bottom of the frame, a sample box 9 drive unit that drives the sample box 9 to reciprocate in the Y-axis direction, and a clamping and fixing unit 23 that clamps and fixes the sample removed from the sample box 9 by the XZ dual-axis manipulator 13. The sample box 9 drive unit is located on the left side of the base plate 1 and is also a conventional combination of a lead screw and a stepper motor, with the lead screw parallel to the Y-axis. Immediately to the right is the sample box 9, and a guide rail at the bottom of the sample box 9 facilitates operations such as moving the sample box 9 and changing samples. Furthermore, in combination with the XZ dual-axis manipulator 13, free movement in the XYZ three-axis directions is achieved. In this embodiment, the XZ dual-axis manipulator 13 adopts an existing manipulator structure, which can clamp the sample tube in the sample box 9 and open the lid of the sample tube after clamping and fixing it. The function is relatively simple to implement, and there are many existing technologies, so this embodiment will not be described in detail.

[0048] The clamping and fixing unit 23 is located on the base plate 1 and to the right of the sample box 9. The clamping and fixing unit 23 also consists of a conventional screw and stepper motor combination. The screw is parallel to the Y-axis; however, the screw utilizes two sections with oppositely directed threads, paired with two sliders with corresponding thread directions. The two sliders have grooves on their opposing surfaces that accommodate the sample tubes. When the stepper motor rotates in the forward direction, the two sliders move relative to each other, clamping the sample tube. When the stepper motor rotates in the reverse direction, the two sliders move away from each other, releasing the clamping of the sample tube.

[0049] The sample staining automatic pipetting device includes an XYZ three-axis manipulator 18 positioned above the frame, a tip collection box 4 positioned at the bottom of the frame, and a tip collection box 4 drive unit that drives the tip collection box 4 back and forth in the Y-axis direction. The tip collection box 4 drive unit is located on the base plate 1 and to the right of the turntable 14. It is also a conventional screw and stepper motor combination, with the screw parallel to the Y-axis. Immediately to the right is the tip collection box 4. A guide rail at the bottom of the tip collection box 4 allows the tip collection box 4 to be removed, making it easy to replace. The tip collection box 4 includes a new tip installation box and a waste tip recovery box, each of which is separated by a front and rear arrangement. Both the new tip installation box and the waste tip recovery box are equipped with several slots for placing tips. In this embodiment, the XYZ three-axis manipulator 18 utilizes an existing manipulator structure, capable of automatically replacing new tips and removing used waste tips. Its functionality is relatively simple to implement, and there are many existing technologies, so this embodiment will not be elaborated on.

[0050] In this embodiment, the XZ dual-axis manipulator 13, the XYZ three-axis manipulator 18 and a combination of several conventional lead screws and stepper motors constitute a driving assembly, all of which are electrically connected to the controller. Specific implementation method:

[0052] When using for the first time, Figure 1 As shown, the controller returns the magazine compartment 10 to its initial position, and sixteen reagent cards are placed in each of the six reagent card magazines 12, and then installed in the interlayer cavity 11 of the magazine compartment 10. The controller also returns the sample box 9 to its installation position to facilitate manual loading or replacement of samples. The tip collection box 4 also returns to its installation position to facilitate manual loading of new tips or removal of used tips, and to empty the waste card collection box 3.

[0053] Then, the controller moves the cartridge compartment 10, aligning the first opening of the first reagent card cartridge 12 on the left side with the reagent card slot 17 of the turntable 14, and starting the reagent card feeding assembly. At the same time, the code scanner 5 scans the code. If the code is scanned, the turntable 14 rotates. If the code is not scanned, the turntable 14 does not rotate. At the same time, the first reagent card ejection assembly 15 is started to eject the card. The test cartridge compartment 10 moves to allow the recovery chamber 8 to arrive at the designated position to receive the reagent card. Repeat the above process. The rotation of the turntable 14 is provided with a time interval to leave time for the automatic sampler and the sample dyeing automatic liquid transfer device to operate. At the same time, the controller also counts. If it is full sixteen, the cartridge compartment 10 moves the distance of one interlayer cavity 11.

[0054] At the same time, the XZ two-axis manipulator 13 of the automatic sample sampling device clamps the sample tube in the sample box 9 and sends it to the clamping and fixing unit 23 for clamping and fixing. After the clamping and fixing unit 23 clamps and fixes it, the XZ two-axis manipulator 13 opens the lid of the sample tube. The XYZ three-axis manipulator 18 of the automatic sample staining pipetting device then pipettes the sample solution into the dilution cup of the reagent card through the tip head for dilution. After dilution, the solution is pipetted into the sample loading window of the reagent card for color development reaction. The XZ two-axis manipulator 13 is then controlled to tighten the lid of the sample tube and send it to its original position. The XYZ three-axis manipulator 18 retracts the tip head and automatically replaces it with a new tip head. The above process is repeated in a cycle.

[0055] When the reagent card that has completed the pipetting reaches the card ejection position, the optical path detection module 22 is allowed to collect data, and the second reagent card ejection component 19 is started to push the reagent card into the waste card collection box 3.

[0056] The first reagent card ejection component 15 sends the reagent card to the turntable 14, the turntable 14 rotates, the sample automatic sampling device and the sample staining automatic pipetting device operate, the optical path detection module 22 collects data, and the second reagent card ejection component 19 completes the card ejection. The above process is continuously cycled, and the entire process is highly automated, which greatly saves labor costs and improves detection efficiency.

[0057] The above is only an embodiment of the present invention. Common knowledge such as the known specific structures and characteristics in the scheme is excessively described here. Ordinary technicians in the relevant field are aware of all common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all existing technologies in the field, and have the ability to apply conventional experimental means before that date. Ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention. These will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A nanoenzyme immunoassay system, characterized in that: The device comprises a frame and a controller, wherein the frame is provided with a reagent card transport device electrically connected to the controller, a sample automatic sampling device, a reagent card installation and detection device, and a sample staining automatic pipetting device; The reagent card installation and detection device includes a turntable and a turntable drive assembly for driving the turntable; the turntable is provided with a plurality of reagent card slots evenly distributed around the center of the circle; The reagent card transport device includes a first guide rail mounted on a frame, a magazine sliding on the first guide rail, an X-axis drive unit for driving the magazine to move horizontally back and forth, and a reagent card feeding assembly; the magazine includes a plurality of interlayer cavities that evenly divide its internal space, and a reagent card magazine mounted in the interlayer cavities, wherein a plurality of reagent cards are stacked up and down in the reagent card magazine; the bottom surfaces of the reagent card magazine and the interlayer cavities are both provided with through grooves communicating with the interior, and the through grooves extend through the front and rear sides of the reagent card magazine and the interlayer cavities; a first opening located on the front side of the reagent card magazine and the interlayer cavity is for allowing the reagent card to pass through and can be opposite to a notch of a reagent card slot on the turntable, and the width of the through groove is smaller than the width of the reagent card; The reagent card feeding assembly includes a first slider, a first slider driving unit that drives the first slider to reciprocate, and a card pushing unit fixed to the slider. The card pushing unit includes a fixed bracket and a rotating block hinged to the fixed bracket and rotating in the vertical direction. The basic state of the rotating block is a vertical state, and the upper end of the rotating block can slide in the through groove and limit the upper end of the rotating block from flipping backward. A spherical counterweight block is provided at the bottom of the rotating block. The lower surface of the reagent card is provided with a QR code, and the frame is provided with a scanner for scanning the QR code, and the scanner is electrically connected to the controller; The reagent card installation detection device further includes a reagent card data acquisition component for acquiring data from the reagent card on the turntable and a reagent card ejection component for ejecting the reagent card from the reagent card slot; The reagent card ejection component includes a first reagent card ejection component for ejecting a reagent card that fails scanning and identification from the reagent card slot and a second reagent card ejection component for ejecting a reagent card that completes data collection from the reagent card slot.

2. A nanoenzyme immunoassay system according to claim 1, characterized in that: A limiting post is provided in the center of the central area of the turntable and at the center of the end position of the reagent card slot. A limiting buckle is provided at the front end of the reagent card. The limiting post is matched with the limiting buckle for engagement.

3. The nanoenzyme immunoassay system according to claim 1, characterized in that: The first reagent card ejection assembly and the second reagent card ejection assembly have the same structure and are both arranged above the turntable, including: a structural bracket fixed to the frame, a second slider, a second slider driving unit that drives the first slider to reciprocate, and a card ejection paddle fixed to the lower end of the second slider, and the card ejection paddle is abutted against the end of the reagent card on the turntable and close to the center end of the turntable.

4. A nanoenzyme immunoassay system according to claim 3, characterized in that: A recovery bin for recovering reagent cards that have failed code scanning and have been returned by the first reagent card return assembly is detachably mounted on the side of the magazine compartment.

5. The nanoenzyme immunoassay system according to claim 3, characterized in that: The frame is provided with a waste card collection box for recovering reagent cards that have completed data collection, and a waste card collection box driving unit for driving the waste card collection box to and fro. The waste card collection box driving unit is electrically connected to the controller.

6. The nanoenzyme immunoassay system according to claim 1, characterized in that: The automatic sample sampling device includes an XZ dual-axis manipulator arranged above the frame, a sample box arranged at the bottom of the frame, a sample box driving unit that drives the sample box to reciprocate in the Y-axis direction, and a clamping and fixing unit that clamps and fixes the sample taken out of the sample box by the XZ dual-axis manipulator.

7. The nanoenzyme immunoassay system according to claim 1, characterized in that: The sample staining automatic pipetting device includes an XYZ three-axis manipulator arranged above the frame, a tip head collection box arranged at the bottom of the frame, and a tip head collection box driving unit that drives the tip head collection box to reciprocate in the Y-axis direction. The tip head collection box includes a new tip head installation box and a waste tip head recovery box that are separately arranged. The new tip head installation box and the waste tip head recovery box are both provided with a plurality of placement slots for placing tip heads.

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