A method for controlling fruit fly pests by using repellents in combination with two-way attractants

Abstract

The application discloses a method for preventing and controlling real fly pests by using repellent and dual sex attractant, wherein the repellent is prepared from soil chenopodium oil and castor oil as active ingredients, and the dual sex attractant is prepared by mixing 100 mu g / ml of N-(3-methylbutyl) formamide, butyl acetate, methyl butyrate, anise aldehyde, beta-caryophyllene and decanal solution with the same mass. The natural plant essential oil is used as the repellent, the types of the natural plant essential oil are various, the structure of the natural plant essential oil is complex, and the repellent has better avoidance effect on the real fly; the dual sex attractant has various structures of substances, and has a wide range of target site effects, can specifically attract male insects and specifically attract female insects, and has a female and male dual attraction compound, so that the prevention and control method of the application can maximize the prevention and control effect on the real fly, and has strong avoidance and attraction effects on the real fly pests, and has the characteristics of low cost, environmental protection, high efficiency, no pesticide residue and the like, and has a wide application prospect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of bio-technology of agroforestry insects, and particularly relates to a method for controlling real fly pests by using repellent and dual sex attractant. BACKGROUND

[0002] Real fly, a general term of Tephritidae in Diptera, belongs to Tephritidae in Diptera, and there are about 500 genera and more than 4500 species in the world. Real fly is a small and medium-sized insect, with a round spherical head, a developed middle thorax, and wing with flower spots. Real fly is a plant-feeding insect, and the larvae are all cryptophagous, which can harm plants in various parts, such as roots, stems, leaves, flowers and fruits. Many species are crop pests, among which the species that harm fruits are particularly important, such as Mediterranean fruit fly, apple fruit fly and cherry fruit fly, which are well-known worldwide. These species are all quarantine objects in the world, and have not been found in China. In China, some species have been found to be important pests of fruits and vegetables such as citrus, pear and melon, such as citrus fruit fly, citrus small fruit fly, melon fruit fly and wolfberry fruit fly.

[0003] Chemical trapping is currently an important means for preventing and controlling real flies on fruit trees and vegetables at home and abroad, but due to its single trapping and low efficiency, the trapped real flies are basically male insects, while female insects can still mate at will, resulting in poor control effect. Multi-mode combined trapping and killing of real flies has become the optimal strategy for real fly control technology. The development of compounds with female and male dual attraction for real flies has become a hot spot for current trapping and killing control, but there is no report on real fly female and male dual attraction products based on plant source substances. SUMMARY

[0004] The purpose of the present application is to provide a method for controlling real fly pests by using repellent and dual sex attractant. The present application is based on the principle of insect chemical ecology, and uses host plant source substances with high female and male real fly trapping performance as the core, and adds an appropriate amount of real fly sex attractant to achieve the effect of female and male dual attraction.

[0005] To achieve the above purpose, the present application adopts the following technical solutions:

[0006] A method for controlling real fly pests by using repellent and dual sex attractant, which is to place the repellent in a rubber head type slow-release carrier with a height of 2 cm, a diameter of 0.5 cm and a depth of 1 cm, drop the dual sex attractant into a PVC capillary tube with an inner diameter of 0.1 cm and a length of 5 cm, and then hang the prepared repellent and dual sex attractant in the orchard in a way of outer dense and inner sparse.

[0007] Further, the repellent is a mixture of 10 g / L Chenopodium ambrosioides essential oil solution and 5 g / L castor essential oil solution with acetone as a solvent, and the mass ratio is 1-3:1-5.

[0008] Further, the dual sex attractant is prepared by mixing 100 μg / ml of N-(3-methylbutyl) formamide, butyl acetate, methyl butyrate, anisic aldehyde, beta-caryophyllene and decanal, respectively, in liquid paraffin as solvent.

[0009] Further, the real fly pests include B. dorsalis, C. capitata, C. capitata and Dacus oleae.

[0010] Further, the specific hanging mode is to hang the repellent at the middle position of the fruit and vegetable garden, 10-20 cm above the top of the protected crops; and then hang the dual sex attractant at a position 10-20 cm above the top of the protected crops and 25-30 m away from the repellent, and symmetrically and linearly arrange the dual sex attractants with the hanging position of the repellent as the center, and the distance between the two dual sex attractants is 50-60 m.

[0011] Further, the number of the hung dual sex attractants is 4 times the number of the hung repellents.

[0012] Further, if the area of the fruit and vegetable garden is large, multi-layer hanging can be performed in the periphery of the hung dual sex attractants in an alternating manner of repellent and dual sex attractant, so as to completely eliminate the real flies in the specific area and achieve the prevention and control of the entire fruit and vegetable garden.

[0013] The functions and effects of the components in the application are as follows:

[0014] N-(3-methylbutyl) formamide is a sex attractant component with strong attraction to male real fly insects.

[0015] Butyl acetate is a compound with strong attraction to male real fly insects.

[0016] Methyl butyrate and anisic aldehyde not only have good attraction to male real fly insects, but also have strong attraction to female insects.

[0017] Beta-caryophyllene and decanal have strong attraction to female real fly insects.

[0018] The application prepares a compound with strong repellent effect on real flies by mixing two plant essential oils in a certain proportion, and prepares a dual sex attractant with complementary effect by mixing N-(3-methylbutyl) formamide (male), butyl acetate (male), methyl butyrate (male and female), anisic aldehyde (male and female), beta-caryophyllene (female) and decanal (female), which can maximally improve the prevention and control effect on real flies due to the specific attraction to male insects, the specific attraction to female insects and the dual sex attractant compounds.

[0019] According to the above technical solution, compared with the prior art, the application has the following beneficial effects:

[0020] (1) The dual sex attractant used in the application has diverse structures of substances, wide target site action, specific attraction to male insects and specific attraction to female insects, and also has female and male dual attraction compounds, which can maximize the control effect on real flies.

[0021] (2) The repellent components of the application are all natural plant essential oils, which are not easy to cause insects to develop resistance, are environmentally friendly, safe and non-polluting.

[0022] (3) The components in the dual sex attractant of the application are all single compounds with strong attraction, and the essential oils in the repellent are multi-component high-efficiency repellent mixtures, and the combination of the two can achieve precise trapping and killing and high-efficiency repellence, and the complementary advantages are extremely significant.

[0023] (4) The dual sex attractant formula used in the application has a high-efficiency attraction effect on real flies, and in combination with the repellent essential oil, the pests can be finally killed by the "push-pull" trap.

[0024] (5) The combined control method has the characteristics of low cost, environmental protection, high efficiency, no pesticide residues, etc.; and the repellent plant essential oil used is derived from weeds or invasive plants, which can turn waste into treasure. DETAILED DESCRIPTION

[0025] In order to make the content of the application more convenient to understand, the technical solutions of the application will be further described below in combination with specific embodiments, but the application is not limited thereto.

[0026] Example 1 Preparation of dual sex attractant and repellent for real flies

[0027] 1.1 Preparation of single component solution

[0028] Preparation of N-(3-methylbutyl) formamide solution: liquid paraffin is used as a solvent to prepare a 10 μg / μl N-(3-methylbutyl) formamide standard solution, then 10 μl is added to 990 μl of liquid paraffin and uniformly mixed to obtain a 100 μg / ml N-(3-methylbutyl) formamide solution;

[0029] Preparation of butyl acetate solution: liquid paraffin is used as a solvent to prepare a 10 μg / μl butyl acetate standard solution, then 10 μl is added to 990 μl of liquid paraffin and uniformly mixed to obtain a 100 μg / ml butyl acetate solution;

[0030] Preparation of butyl acetate solution: liquid paraffin is used as a solvent to prepare a 10 μg / μl butyl acetate standard solution, then 10 μl is added to 990 μl of liquid paraffin and uniformly mixed to obtain a 100 μg / ml butyl acetate solution;

[0031] Preparation of anisaldehyde solution: 10 μg / μl of anisaldehyde standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of anisaldehyde solution;

[0032] Preparation of β-caryophyllene solution: 10 μg / μl of β-caryophyllene standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of β-caryophyllene solution;

[0033] Preparation of decanal solution: 10 μg / μl of decanal standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of decanal solution;

[0034] 1.2 Preparation of bethyl sex attractant

[0035] The obtained 100 μg / ml N-(3-methylbutyl) formamide solution, butyl acetate solution, methyl butyrate solution, anisaldehyde solution, β-caryophyllene solution and decanal solution were mixed in the same mass to prepare sex attractant A;

[0036] 1.3 Preparation of bethyl repellent

[0037] Chenopodium ambrosioides essential oil and castor oil were mixed in acetone at 10 g / L and 5 g / L respectively, then the two solutions were mixed in a mass ratio of 3:2 to prepare repellent 1.

[0038] Example 2 Preparation method of bethyl sex attractant and repellent

[0039] 1.1 Preparation of single component solution

[0040] Preparation of N-(3-methylbutyl) formamide solution: 10 μg / μl of N-(3-methylbutyl) formamide standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of N-(3-methylbutyl) formamide solution;

[0041] Preparation of butyl acetate solution: 10 μg / μl of butyl acetate standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of butyl acetate solution;

[0042] Preparation of methyl butyrate solution: 10 μg / μl of methyl butyrate standard solution was prepared using liquid paraffin as solvent, then 10 μl was added into 990 μl of liquid paraffin and uniformly mixed to obtain 100 μg / ml of methyl butyrate solution;

[0043] Preparation of anisaldehyde solution: 10 μg / μl of anisaldehyde standard solution was prepared using liquid paraffin as solvent, then 10 μl was added into 990 μl of liquid paraffin and uniformly mixed to obtain 100 μg / ml of anisaldehyde solution;

[0044] Preparation of β-caryophyllene solution: 10 μg / μl of β-caryophyllene standard solution was prepared using liquid paraffin as solvent, then 10 μl was added into 990 μl of liquid paraffin and uniformly mixed to obtain 100 μg / ml of β-caryophyllene solution;

[0045] 1.2 Preparation of bethyl sex attractant

[0046] The obtained 100 μg / ml N-(3-methylbutyl) formamide solution, butyl acetate solution, methyl butyrate solution, anisaldehyde solution and β-caryophyllene solution were mixed in the same mass to prepare the sex attractant B of the application;

[0047] 1.3 Preparation of bethyl repellent

[0048] The chenopodium pumilo essential oil and the castor essential oil were respectively added into acetone in an amount of 10 g / L and 5 g / L, then the two solutions were mixed in a mass ratio of 1:1 to prepare the repellent 2.

[0049] Example 3 Preparation method of bethyl sex attractant and repellent

[0050] 1.1 Preparation of single component solution

[0051] Preparation of N-(3-methylbutyl) formamide solution: 10 μg / μl of N-(3-methylbutyl) formamide standard solution was prepared using liquid paraffin as solvent, then 10 μl was added into 990 μl of liquid paraffin and uniformly mixed to obtain 100 μg / ml of N-(3-methylbutyl) formamide solution;

[0052] Preparation of butyl acetate solution: 10 μg / μl of butyl acetate standard solution was prepared using liquid paraffin as solvent, then 10 μl was added into 990 μl of liquid paraffin and uniformly mixed to obtain 100 μg / ml of butyl acetate solution;

[0053] Preparation of methyl butyrate solution: 10 μg / μl of methyl butyrate standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of methyl butyrate solution;

[0054] Preparation of anisaldehyde solution: 10 μg / μl of anisaldehyde standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of anisaldehyde solution;

[0055] Preparation of decanal solution: 10 μg / μl of decanal standard solution was prepared using liquid paraffin as solvent, then 10 μl was added to 990 μl of liquid paraffin and mixed uniformly to obtain 100 μg / ml of decanal solution;

[0056] 1.2 Preparation of bethyl sex attractant

[0057] The obtained 100 μg / ml N-(3-methylbutyl) formamide solution, butyl acetate solution, methyl butyrate solution, anisaldehyde solution and decanal solution were mixed in the same mass to prepare sex attractant C;

[0058] 1.3 Preparation of bethyl repellent

[0059] The soil chenopodium essential oil and castor essential oil were mixed with acetone at 10 g / L and 5 g / L respectively, then the two solutions were mixed in a mass ratio of 1:2 to prepare repellent 3.

[0060] Example 4: Indoor determination effect of bethyl repellent and sex attractant

[0061] The Y-type olfactometer was used to determine the orientation behavior response of B. oleae to the compound (the Y-type olfactometer used was improved according to the basic principle of olfactometer and the authorized patent number ZL201020239767.7). The main arm of the Y-type tube was 20 cm, the length of each arm was 10 cm, and the angle between the two arms was 90℃. The end of the two-arm tube was connected with a distilled water bottle and an activated carbon bottle in sequence by silica gel tube, and finally the activated carbon bottle was opened to the atmosphere, and the insect release bottle was connected to the air suction device with gauze. The filter paper strip was cut into 5 cm x 1 cm and folded into a "V" shape and placed in the glass tube at the entrance of the trap.

[0062] The Y-shaped olfactometer was inclined at 30° and the temperature was controlled at 25±2℃. 100 μl of the sex attractant formula prepared in Examples 1-3 and the repellent were dropped uniformly on the filter paper strip and placed in the odor source bottle on one side, and the filter paper with 100 μl of liquid paraffin was dropped as a reference on the other side. The air flow rate was 1 L / min, and the air flow in the two tubes was filtered by activated carbon and humidified by distilled water.

[0063] The hatched 1-3 instar B. dorsalis adults were placed in an insect release bottle, 30 (15 males and 15 females) per test, repeated 3 times, and after each test, the inner and outer walls of the Y-shaped tube were wiped with a cotton ball dipped in anhydrous ethanol and dried, and then the two arms of the Y-shaped tube were exchanged with the odor source bottle and the control to eliminate the influence of the geometric error of the two arms on the behavior of the fruit flies. The test insects were placed in the insect release bottle of the Y-shaped tube to start the test, and each test insect was allowed to crawl through the selection arm at 1 / 2, and stayed for more than 10 s, which was calculated as a selection. The B. dorsalis staying in the main arm of the Y-shaped tube was regarded as no reaction. The experimental results within 30 min were counted and the repellency rate and the attraction rate were calculated according to the following formula.

[0064] Repellency rate / % = (number of insects in the control tube-number of insects in the treated tube) / total number of insects x 100;

[0065] Attraction rate / % = (number of insects in the treated tube-number of insects in the control tube) / total number of insects x 100.

[0066] The repellency rate results show that the repellents obtained in Examples 1-3 have repellency rates of 80.22%, 70.00% and 64.44% on B. dorsalis, respectively, indicating that they all have very good repellent effect on fruit flies. The indoor behavior selection test on other fruit flies also proves that they have very good repellent effect.

[0067] The attraction rate results show that the bisexual attractants obtained in Examples 1-3 have attraction rates of 100%, 76.67% and 72.22% on B. dorsalis, respectively, indicating that the obtained bisexual attractants have very good bisexual attractant effect, and the bisexual attractant effect of formula A is significantly higher than that of formula B and formula C.

[0068] Example 5 Field control effect of B. dorsalis

[0069] The test site was selected in Yuxi fruit and vegetable base in Fuqing City, Fujian Province, and the crop was guava. A cylindrical trap was used as a trapping device, and the repellents and bisexual attractants obtained in Examples 1-3 were hung on the upper part of the guava at 10 cm, and each 5 mu of guava garden was used as a test area, 4 repetitions were set, and different treatments were randomly arranged. The repellent was hung in the middle of each test area, one for each, and the bisexual attractant was hung around each test area, four for each. The number of B. dorsalis in the guava garden was investigated, that is, the number of B. dorsalis in the trap was investigated at 3d, 7d and 14d, respectively. Finally, the number of live insects in the treatment and control areas before and after treatment was calculated to calculate the control effect, and the results are shown in Table 1. The commercially available conventional male insect trap without repellent was used as a blank control (CK). An isolation belt was provided in the middle.

[0070]

[0071] Table 1 Field control effect of the control method of the application on B. dorsalis

[0072]

[0073] The results are shown in Table 1, and the control effects of the repellent and the dual sex attractant on B. dorsalis are 63.41%, 70.80% and 80.26% at 3d, 7d and 14d, respectively, which shows that a good control effect can be achieved without applying pesticides. In the blank control, the number of B. dorsalis increases slightly with time and is basically stable.

[0074] The above description is only the preferred embodiment of the present application, and any equivalent changes and modifications made within the scope of the present application should be included in the scope of the present application.

Claims

1. A method for controlling fruit fly pests by combining a repellent and a bisexual attractant, characterized in that: Specifically, the repellent is placed in a rubber-head slow-release carrier with a height of 2 cm, a diameter of 0.5 cm, and a depth of 1 cm, and the bisexual attractant is dripped into a PVC capillary tube with an inner diameter of 0.1 cm and a length of 5 cm. The repellent and bisexual attractant are then hung in the orchard in a dense outer layer and sparse inner layer. The specific hanging method is to hang the repellent in the middle of the fruit and vegetable garden, 10-20 cm above the protected crops; then hang the bisexual attractant 10-20 cm above the protected crops, 25-30 m away from the repellent, and arrange them symmetrically and in a straight line with the repellent hanging position as the center, and the distance between the two bisexual attractants is 50-60 m; The number of hung two-sex attractants is 4 times the number of hung repellents; Around the hanging bisexual attractants, multiple layers of repellents and bisexual attractants are hung alternately to achieve control over the entire fruit and vegetable garden. The repellent is prepared by mixing a 10 g / L schizonepeta essential oil solution and a 5 g / L castor essential oil solution in an acetone solvent at a mass ratio of 3:

2. The preparation method of the repellent comprises: adding 10 g / L schizonepeta essential oil and 5 g / L castor essential oil to acetone, mixing the mixture, and then mixing the two solutions at a mass ratio of 3:2 to prepare the repellent. The amphoteric attractant is prepared by using liquid paraffin as a solvent, preparing 100 μg / ml single-component solutions of N-(3-methylbutyl)formamide, butyl acetate, methyl butyrate, anisaldehyde, β-caryophyllene and decanal, and then mixing the solutions in equal amounts. The preparation method of the amphoteric attractant is as follows: (1) Preparation of single-component solution Preparation of N-(3-methylbutyl)formamide solution: Prepare a 10 μg / μl N-(3-methylbutyl)formamide standard solution in liquid paraffin as the solvent. Then, add 10 μl of the solution to 990 μl of liquid paraffin and mix well to obtain a 100 μg / ml N-(3-methylbutyl)formamide solution. Preparation of butyl acetate solution: Prepare a 10 μg / μl butyl acetate standard solution using liquid paraffin as solvent. Then, add 10 μl of the solution to 990 μl of liquid paraffin and mix well to obtain a 100 μg / ml butyl acetate solution. Preparation of methyl butyrate solution: Prepare a 10 μg / μl methyl butyrate standard solution using liquid paraffin as solvent. Then, add 10 μl of the solution to 990 μl of liquid paraffin and mix well to obtain a 100 μg / ml methyl butyrate solution. Preparation of anisaldehyde solution: Prepare a 10 μg / μl anisaldehyde standard solution using liquid paraffin as solvent. Then, add 10 μl of the solution to 990 μl of liquid paraffin and mix well to obtain a 100 μg / ml anisaldehyde solution. Preparation of β-caryophyllene solution: Prepare a 10 μg / μl β-caryophyllene standard solution using liquid paraffin as solvent. Then, add 10 μl of the solution to 990 μl of liquid paraffin and mix well to obtain a 100 μg / ml β-caryophyllene solution. Preparation of decanal solution: Prepare a 10 μg / μl decanal standard solution using liquid paraffin as solvent. Then, add 10 μl of the solution to 990 μl of liquid paraffin and mix well to obtain a 100 μg / ml decanal solution. (2) Preparation of fruit fly bisexual attractant: The obtained 100 μg / ml N-(3-methylbutyl)formamide solution, butyl acetate solution, methyl butyrate solution, anisaldehyde solution, β-caryophyllene solution and decanal solution were mixed in equal amounts to prepare a bisexual attractant; The fruit fly pest is the oriental fruit fly.

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