Use of trehalose as a prebiotic for inhibiting pathogenic microorganisms

Abstract

The present invention relates to a new use of trehalose. More specifically, the present invention relates to a new use of trehalose for promoting scalp health. Accordingly, the present invention provides the use of trehalose for use as a prebiotic for inhibiting pathogenic microorganisms when applied on the external surface of the human body.

Description

Technical Field

[0001] This invention relates to novel uses of trehalose. More specifically, this invention relates to novel uses of trehalose as a prebiotic to promote scalp health. Background Technology

[0002] Prebiotic compositions are well known as both food compositions and topical compositions. Prebiotic compositions are believed to provide health benefits when utilized by host microorganisms (often known as good bugs). Therefore, prebiotic compositions utilize such microorganisms, which are present in the human body in any way, either on a local surface or in the gut.

[0003] Topical prebiotic compositions are a subject of interest for both industrial and academic research. Prebiotic compositions intended for topical application and utilization by microorganisms reside on local surfaces, such as the skin and scalp, thereby providing benefits associated with these surfaces.

[0004] There are prebiotic skin compositions and methods known in the art.

[0005] US 2013115610 (P&G, 2013) describes a method for identifying a test reagent that exhibits prebiotic activity against human skin symbiotic microorganisms, and a composition comprising such a reagent. The method includes providing a test reagent, human skin symbiotic microorganisms, and a test culture in a minimal carbon medium. This method provides a time-efficient and cost-effective approach to predicting the in vivo prebiotic activity of a test reagent against skin symbiotic microorganisms.

[0006] US 2007190004 (Henkel, 2007) describes a method for promoting prebiotic activity on the skin, the method comprising contacting the skin with an effective amount of a substance having prebiotic activity, the substance being selected from plant extracts, glyceryl monoalkyl ethers and fatty acid esters or a combination of plant extracts and glyceryl monoalkyl ethers, thereby promoting the growth of desired skin microorganisms and inhibiting the growth of unwanted skin microorganisms.

[0007] Numerous microorganisms reside on human skin or scalp. Some of them are harmless. However, a small number of them can cause various diseases or create undesirable conditions, such as Staphylococcus aureus, Malassezia restricta, and Acinetobacter.

[0008] One of these undesirable conditions is dandruff. This is a common scalp condition affecting many people, characterized by shedding, itching, and visible flakes on the scalp. It has been found that in cases of dandruff, the level of Malassezia restricta is significantly higher than in healthy scalps. Therefore, the inventors believe that reducing the level of Malassezia restricta is one of the effective methods for treating dandruff.

[0009] Therefore, there remains a need for compositions or materials to reduce such unwanted microorganisms. In particular, compositions or materials capable of significantly reducing levels of Malassezia are currently needed. Summary of the Invention

[0010] In a first aspect, the present invention provides the use of a disaccharide, which, when applied to the external surface of the human body, acts as a prebiotic that inhibits pathogenic microorganisms.

[0011] In a second aspect, the present invention provides the use of trehalose, which, when applied to the external surface of the human body, acts as a prebiotic that inhibits pathogenic microorganisms.

[0012] In a third aspect, the present invention provides the use of trehalose, which, when applied to the human scalp, inhibits restrictive Malassezia in the presence of Staphylococcus aureus.

[0013] In a fourth aspect, the present invention provides the use of trehalose as a prebiotic for suppressing dandruff.

[0014] In a fifth aspect, the present invention provides the use of a prebiotic topical composition comprising trehalose, which, when applied to the external surface of the human body, inhibits pathogenic microorganisms.

[0015] In a sixth aspect, the present invention provides a method for applying a composition comprising trehalose, wherein the composition is applied as a prebiotic for inhibiting pathogenic microorganisms to the outer surface of the human body.

[0016] These and all other aspects of the invention will become more apparent when considering the following detailed description and examples. Detailed Implementation

[0017] These and other aspects, features, and advantages will become apparent to those skilled in the art upon reading the following detailed description and appended claims. For the avoidance of doubt, any feature of one aspect of the invention may be used in any other aspect of the invention. The word “comprising” means “including” but not necessarily “consisting of” or “composed of”. In other words, the listed steps or options are not necessarily exhaustive. It should be noted that the embodiments given in the following description are intended to illustrate the invention and not to limit the invention to these embodiments alone. Similarly, unless otherwise stated, all percentages are weight / weight percentages. Unless in the examples and comparative examples, or where otherwise expressly indicated, all numerical values ​​representing the amount of material or the conditions of reaction, the physical properties of the material, and / or the use thereof in this specification and claims should be understood to be modified by “about”. Numerical ranges expressed in the form of “from x to y” should be understood to include both x and y. When multiple preferred ranges are described in the form of “from x to y” for a particular feature, it should be understood that all ranges combining different endpoints should also be considered.

[0018] The disclosure of the invention found herein is intended to cover all embodiments found in the claims that reference each other multiple times, without regard to the fact that the claims may be found to be without multiple references or redundant.

[0019] In cases where features of a particular aspect of the invention (e.g., the compositions of the invention) are disclosed, such disclosure is also considered applicable to any other aspect of the invention (e.g., the methods of the invention) with necessary modifications.

[0020] As used herein, “personal care composition” refers to compositions intended for topical application to the skin of mammals, particularly humans. Such compositions are typically categorized as leave-on or rinse-off. These compositions are formulated into products specifically applied to the human body for aesthetic purposes, but also capable of providing cleansing, odor control, or general aesthetic effects. The compositions of the present invention can be in the form of liquids, lotions, creams, foams, scrubs, gels, or toners, or applied using an apparatus or through a mask or pad. Non-limiting examples of such compositions include leave-on skin lotions, creams, antiperspirants, deodorants, lipsticks, foundation creams, mascaras, sunscreens, and sunblock lotions. The compositions of the present invention are preferably leave-on compositions. As used herein, “skin” refers to the skin on the face and body (e.g., neck, chest, back, arms, armpits, hands, legs, buttocks, and scalp), particularly the sun-exposed portions.

[0021] For the purposes of this invention, the term "prebiotic" refers to an ingredient or composition used by other symbiotic microorganisms (beneficial insects) present on the skin, which provides skin benefits, including the inhibition of pathogenic microorganisms.

[0022] Many microorganisms reside on localized surfaces of the human body, such as the skin, scalp, or mouth. Not all of these microorganisms are harmless. Some exhibit adverse effects on the skin, scalp, or mouth.

[0023] This invention provides a novel use for disaccharides, which, when applied to the external surface of the human body, serve as prebiotics to inhibit pathogenic microorganisms.

[0024] Disaccharides are sugars formed by two monosaccharides linked by a glycosidic bond.

[0025] In the case of this invention, the preferred disaccharide is trehalose. Trehalose has the following structure:

[0026]

[0027] Trehalose is a disaccharide formed by a 1,1-glycosidic bond between two α-glucose units.

[0028] The present invention preferably discloses the use of trehalose, which, when applied to the external surface of the human body, acts as a prebiotic to inhibit pathogenic microorganisms.

[0029] The inventors have discovered that Staphylococcus hominis, present on the surface of the skin / scalp, uses trehalose to inhibit pathogenic microorganisms.

[0030] Therefore, the present invention also provides the use of trehalose, which, when applied to the external surface of the human body, inhibits pathogenic microorganisms in the presence of Staphylococcus aureus.

[0031] Preferably, the outer surface comprises keratinocytes. Most preferably, the outer surface is human skin and / or scalp.

[0032] For the purposes of this invention, the preferred pathogenic microorganisms are Malassezia restricta, Staphylococcus aureus, and / or Acinetobacter bacillus.

[0033] It has been found that the level of Malassezia restricta is significantly higher in dandruff-prone scalps compared to healthy scalps. We have found that the disaccharide, preferably trehalose, as a prebiotic is effective against Malassezia restricta, thereby inhibiting dandruff and promoting scalp health. Preferably, when applied to the human scalp, trehalose inhibits Malassezia restricta in the presence of Staphylococcus aureus. Therefore, the present invention also provides the use of trehalose for inhibiting dandruff.

[0034] We also found that other microorganisms that typically reside on human skin, such as Staphylococcus aureus and / or Acinetobacter, are associated with one or more undesirable effects (diseases).

[0035] Therefore, the present invention also provides the use of a prebiotic topical composition comprising trehalose, which, when applied to the external surface of the human body, acts as a prebiotic for inhibiting pathogenic microorganisms.

[0036] The composition preferably contains 0.001% to 10% trehalose by weight. More preferably, the composition contains 0.01% to 8% trehalose, and even more preferably 0.1% to 7% trehalose, and most preferably 0.1% to 5% trehalose by weight.

[0037] The present invention also provides a method for applying a composition comprising trehalose, wherein the composition is applied as a prebiotic for inhibiting pathogenic microorganisms to the outer surface of a human body. Preferably, the pathogenic microorganism is Malassezia restricta, and the outer surface is the human scalp.

[0038] Preferably, the topical composition is a cosmetic composition. Alternatively, the topical composition is a pharmaceutical or drug composition.

[0039] The compositions of the present invention may be in the form of wash-free or wash-off compositions.

[0040] A rinse-free composition preferably refers to a composition that does not require removal or rinsing from the body after application. When the composition is in the form of a rinse-free composition, it is preferably a hair lotion, hair serum, deodorant (stick, roller, or spray), hand sanitizer, body lotion, skin cream, or body spray. A rinse-free composition is distinguished from compositions that are applied to the skin and subsequently removed, either shortly after or during application, by washing, rinsing, or wiping. Typically, the surfactants used in the rinse-off composition have physicochemical properties that give them the ability to produce easily rinsed foam / lather during use; they can consist of mixtures of anionic, cationic, amphoteric, and nonionic surfactants.

[0041] The topical composition is preferably in the form of an emulsion, which can be an oil-in-water emulsion or an oil-in-water emulsion. In some embodiments, the skin care composition is an oil-in-water emulsion. Another form is a cream, including creams having a vanishing cream base. The vanishing cream base is a vanishing cream base containing 5% to 40% by weight of fatty acids and 0.1% to 20% by weight of soap. In some embodiments, in such creams, the fatty acids are essentially a mixture of stearic acid and palmitic acid, and the soap is a potassium salt of the fatty acid mixture, although other counterions and mixtures thereof may be used. Typically, the fatty acids in the vanishing cream base are prepared using hystric acid, which is essentially (typically 90% to 95%) a mixture of stearic acid and palmitic acid. A typical hystric acid contains 52% to 55% palmitic acid and 45% to 48% stearic acid based on the total palmitic acid-stearic acid mixture. Therefore, the contents of the hystric acid used to prepare the vanishing cream base and its soap are within the scope of this invention. In some embodiments, the skin care composition contains more than 7%, or more than 10%, or more than 12% fatty acids.

[0042] Alternatively, the topical composition can be formulated into a disposable personal care towel product.

[0043] In some embodiments, the composition is non-solid. As used herein, the term "non-solid" refers to the viscosity of the composition, for example, using a Brookfield DV-I+ viscometer (20 RPM, RV6, 30 seconds, 20...). S C) The measured viscosity. In some embodiments, at 20°C, the viscosity is 1 Pas to 500 Pas, alternatively 1 Pas to 200 Pas, alternatively 2 Pas to 100 Pas, alternatively 3 Pas to 50 Pas. In some embodiments, the anionic surfactant is present in an amount of up to 5% by weight of the composition, alternatively 0.01% by weight to 4% by weight, alternatively 0.01% by weight to 3% by weight, alternatively 0.01% by weight to 2% by weight, or alternatively substantially absent (less than 1%, or less than 0.1%, or less than 0.01%) in the leave-on skincare composition. In some embodiments, the total level of surfactant in the skincare composition does not exceed 10%, alternatively less than 8%, or alternatively up to 5%.

[0044] If the composition is in the form of a deodorant, it may preferably contain a conventional deodorant matrix as a cosmetically acceptable matrix. A deodorant is a product in the form of a stick, roller, or propellant medium, used for personal deodorization, such as application to the armpit or underarm area, and may or may not contain antiperspirant active ingredients. Typically, deodorant compositions can be in the form of a robust solid, a soft solid, a gel, a cream, or a liquid, and are dispensed using an applicator suitable for the physical properties of the composition. Typically, deodorant compositions delivered by roller application contain a liquid carrier. Such liquid carriers may be hydrophobic or contain a mixture of both hydrophilic and hydrophobic liquids. They may be in the form of emulsions or microemulsions. The liquid carrier or mixture of carriers typically comprises 30% to 95% by weight, and in many cases 40% to 80% by weight. Typically, hydrophobic liquid carriers may contain one or more materials selected from chemical categories having a melting point not exceeding 25°C and a boiling point of at least 100°C, such as siloxanes, hydrocarbons, branched aliphatic alcohols, esters, and ethers. Typically, the hydrophilic carrier liquid that may be used in the compositions herein comprises water and / or a monohydric alcohol or a polyhydric alcohol or a homologue miscible with water. Polyhydric alcohols typically include ethylene glycol or propylene glycol, or homologues such as diethylene glycol that may be used. In addition, other suitable carriers and components for the deodorant composition may be added.

[0045] A wash-off composition preferably refers to a composition that, after application of, for example, a shampoo, handwashing composition, or facial cleanser, is intended / needs to be removed from the body by washing with a solvent, preferably water. In the case of a wash-off composition, the cosmetically acceptable base preferably additionally contains a surfactant.

[0046] For example, if the composition is in the form of a shampoo, in addition to water, the cosmetically acceptable matrix preferably contains anionic surfactants, such as alkyl sulfates and / or ethoxylated alkyl sulfate surfactants. Such anionic surfactants are preferably present at levels of 1% to 20% by weight, more preferably 2% to 16% by weight, and even more preferably 3% to 16% by weight. Preferred alkyl sulfates are C8-18 alkyl sulfates, more preferably C12-18 alkyl sulfates, and preferably in the form of salts having solubilizing cations such as sodium, potassium, ammonium, or substituted ammonium.

[0047] Preferably, the topical composition comprises a cosmetically acceptable matrix. Examples of ingredients that can be used as a cosmetically acceptable matrix include water, fatty acids, soaps (salts of fatty acids), alcohols, and mixtures thereof.

[0048] In some embodiments, the skin care composition of the present invention further includes a cosmetically acceptable carrier. In some embodiments, where the personal care composition is a skin care composition, the cosmetically acceptable carrier is a lipophilic carrier that is liquid at a temperature of 40°C.

[0049] According to some embodiments of the present invention, the amount of a cosmetically acceptable carrier in the skin care composition may vary. In some embodiments, the amount of a cosmetically acceptable carrier in the skin care composition may be from 1% by weight to 99.9% by weight of the composition. Alternatively, the amount of a cosmetically acceptable carrier in the skin care composition may be from 70% by weight to 95% by weight of the composition. Alternatively, the amount of a cosmetically acceptable carrier may be from 80% by weight to 90% by weight of the composition. Alternatively, the amount of a cosmetically acceptable carrier may be from 5% by weight to 99.9% by weight of the composition. Alternatively, the amount of a cosmetically acceptable carrier may be from 10% by weight to 99.9% by weight of the composition. Alternatively, the amount of a cosmetically acceptable carrier may be from 15% by weight to 99.9% by weight of the composition.

[0050] Cosmetic-acceptable carriers suitable for the composition include water, emollients, fatty acids, fatty alcohols, thickeners, and combinations thereof.

[0051] In some embodiments, the cosmetically acceptable carrier for the skincare composition is aqueous and comprises a W / O or O / W type water and oil emulsion or a W / O / W type multiple emulsion. When present in the composition, water may be present in an amount from 5% to 95% by weight of the skincare composition. Alternatively, water may be present in an amount from 20% to 70% by weight of the skincare composition.

[0052] In some embodiments, the cosmetically acceptable carrier is an emollient material. The emollient material may be in the form of silicone oil, natural or synthetic esters, hydrocarbons, alcohols, and fatty acids. The amount of the emollient material may range from 0.1% by weight to 95% by weight of the composition.

[0053] Silicone oils can be volatile or non-volatile. As used herein, the term "volatile" refers to those materials that have a measurable vapor pressure at ambient temperature.

[0054] According to some embodiments of the present invention, the volatile silicone oil suitable for skin care compositions may be a cyclic (cyclic methyl silicone) or linear polydimethylsiloxane containing 3 to 9 silicon atoms. In one embodiment, the linear polydimethylsiloxane contains 5 to 6 silicon atoms.

[0055] Suitable non-volatile silicone oils for use in compositions include polyalkylsiloxanes, polyalkylarylsiloxanes, and polyether siloxane copolymers. Non-volatile polyalkylsiloxanes available herein include, for example, those having a concentration of 5 × 10⁻⁶ at 25°C. 6 up to 0.1m 2 Polydimethylsiloxane with a viscosity of / s. In some embodiments, a non-volatile silicone oil suitable for use in skin care compositions is one with a viscosity of 1×10⁻⁶ at 25°C. 5 Up to 4×10 4 m 2 Polydimethylsiloxane with a viscosity of / s. Another class of non-volatile silicone oils suitable for use in skin care compositions are emulsified and non-emulsified polysiloxane elastomers, for example, as polydimethylsiloxane / vinyl dimethicone crosspolymers available as Dow Corning 9040, General Electric SFE 839 and Shin-Etsu KSG-18.

[0056] Another type of non-volatile silicone oil suitable for use in skin care compositions is silicone wax, such as Silwax WS-L (polydimethylsiloxane copolyol laurate), which can also be useful.

[0057] Ester emollients may include alkyl esters of saturated fatty acids having 10 to 24 carbon atoms. Examples include, but are not limited to, docosyl neopentanoate, isononyl isononanoate, isopropyl myristate, and octyl stearate. In another example, ester emollients suitable for use in skin care compositions include ether-esters, such as fatty acid esters of ethoxylated saturated fatty alcohols.

[0058] In another example, suitable ester emollients include polyol esters. Examples include, but are not limited to, ethylene glycol mono- and di-fatty acid esters, diethylene glycol mono- and di-fatty acid esters, polyethylene glycol (200-6000) mono- and di-fatty acid esters, propylene glycol mono- and di-fatty acid esters, polypropylene glycol 2000 stearate, ethoxylated propylene glycol stearate, glycerol mono- and di-fatty acid esters, polyglycerol poly-fatty acid esters, ethoxylated glycerol stearate, 1,3-butanediol mono- and 1,3-butanediol distearate, polyoxyethylene polyol fatty acid esters, sorbitol fatty acid esters, and polyoxyethylene sorbitol fatty acid esters are satisfactory polyol esters. Particularly useful are neopentyl glycol esters of pentaerythritol, trimethylolpropane, and C1-C30 alcohols. In another example, according to some embodiments of the invention, ester emollients suitable for use in skin care compositions include wax esters, such as beeswax, cetylene, and tribehenin wax.

[0059] In some skin care compositions, natural ester emollients are based on monoglycerides, diglycerides, and triglycerides. Representative glycerides include, but are not limited to, sunflower seed oil, cottonseed oil, borage oil, borage seed oil, primrose oil, castor oil and hydrogenated castor oil, rice bran oil, soybean oil, olive oil, safflower oil, sheabutter, simondyl oil, and combinations thereof.

[0060] Some skin care compositions may contain suitable hydrocarbons, including but not limited to petrolatum, mineral oil, C11 to C13 isoalkanes, polybutene, and especially isohexadecane, commercially available from Presperse Inc. as Permethyl 101A. Fatty acids having 10 to 30 carbon atoms may also be suitable. Examples of such fatty acids are nonanoic acid, lauric acid, myristic acid, palmitic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, hydroxystearic acid, and behenic acid, and mixtures thereof.

[0061] In some embodiments of the skin care composition, a cosmetically acceptable carrier is a fatty alcohol having 10 to 30 carbon atoms. Suitable fatty alcohols include, but are not limited to, stearyl alcohol, lauryl alcohol, myristyl alcohol, oleyl alcohol, and cetyl alcohol, or mixtures thereof.

[0062] Thickeners can be used as a cosmetically acceptable carrier component in skincare compositions. Typical thickeners include cross-linked acrylates (e.g., Carbopol 982(R)), hydrophobically modified acrylates (e.g., Carbopol 1382(R)), polyacrylamides (e.g., Sepigel 305(R)), acryloylmethylpropane sulfonic acid / salt polymers and copolymers (e.g., Aristoflex HMB(R) and AVC(R)), cellulose derivatives, and natural gums. Available cellulose derivatives include sodium carboxymethyl cellulose, hydroxypropyl methocellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, ethyl cellulose, and hydroxymethyl cellulose. Natural gums suitable for use in skincare compositions according to the invention include, but are not limited to, guar gum, xanthan gum, sclerotium, carrageenan, pectin, and combinations of such gums. Inorganic substances can also be used as thickeners, particularly clays such as bentonite and lithium montmorillonite, fumed silica, talc, calcium carbonate, and silicates such as magnesium aluminum silicate (Veegum(R)).

[0063] The amount of thickener may be from 0.0001% to 10%, or alternatively from 0.001% to 5%.

[0064] In some embodiments, the skin care composition contains a surfactant. When present, the total concentration of the surfactant can be from 0.1% to 90%, or alternatively from 0.1% to 80%. The amount of surfactant depends on a variety of factors, including, but not limited to, the type of personal care product. Surfactants are selected from anionic, nonionic, cationic, and amphoteric active substances. In some embodiments, the nonionic surfactant is one of those having a C1 group condensed with 2 to 100 moles of ethylene oxide or propylene oxide per mole of hydrophobic material. 10 -C 20 Fatty alcohols or acid hydrophobic compounds; C2-C10 alkylphenols condensed with 2 to 20 moles of olefinic oxygen; mono- and di-fatty acid esters of ethylene glycol; mono- and di-fatty acid glycerides; dehydrated sorbitol mono- and di-C8 ...

[0065] According to some embodiments of the present invention, suitable amphoteric surfactants for use in skin care compositions include cocoaminopropyl betaine, C12-C20 trialkyl betaine, sodium lauroamphoacetate, and sodium laurodiamphoacetate. According to some embodiments of the present invention, suitable anionic surfactants for use in skin care compositions include soaps, alkyl ether sulfates and sulfonates, alkyl sulfates and sulfonates, alkylbenzene sulfonates, alkyl and dialkyl sulfosuccinates, and C8-C... 20 Acyl hydroxyethyl sulfonate, C8-C20 alkyl ether phosphate, C8-C20 sarcosine salt, C8-C20 acyl lactyl lactate, sulfoacetate, and combinations thereof. However, anionic surfactants are irritating to the skin, and the skin care compositions of the present invention preferably do not contain anionic surfactants, i.e., contain less than 1%, preferably less than 0.5%, of anionic surfactants.

[0066] In some embodiments, the skin care composition further comprises 0.01% to 2% of a rheology modifier. In some embodiments, the rheology modifier is selected from silica such as fumed silica or hydrophilic silica and clays such as magnesium aluminum silicate, bentonite, lithium montmorillonite, laponite, and mixtures thereof.

[0067] In some embodiments, cosmetic compositions, particularly skin care compositions, contain sunscreen agents. UV-B sunscreens may be selected from the categories of cinnamic acid, salicylic acid, diphenylacrylate, or derivatives thereof. UV-B sunscreens may include one or more of the following: octyl salicylate, 3,3,5-trimethylcyclohexyl-2-hydroxybenzoate, ethylhexyl salicylate, 2-ethylhexyl-2-cyano-3,3-diphenyl-2-acrylate, or 2-ethylhexyl-4-methoxycinnamate (also known as octyl methoxycinnamate or "OMC"). Such UV-B sunscreens are generally commercially available, for example, from Octisalate. TM (octyl salicylate), Homosalate TM (3,3,5-Trimethylcyclohexyl-2-hydroxybenzoate), NeoHeliopan TM (A range of organic UV filters, including OMC (Neo Heliopan AV) TM ) and ethylhexyl salicylate (Neo Heliopan OS TM Octocrylene TM and Milestab 3039 TM (2-Ethylhexyl-2-cyano-3,3-diphenyl-2-acrylate) or Parsol MCX TM (2-Ethylhexyl-4-methoxycinnamate). The amount of UV-B sunscreen oil in the personal care composition can be from about 0.1% by weight to about 20% by weight.

[0068] The personal care composition may further comprise about 0.1% to about 10% by weight of UV-A sunscreen oil. The personal care composition of the present invention, incorporating UV-A sunscreen oil, exhibits a significantly higher UVAPF compared to compositions lacking cyclic carboxylic acids. The UV-A sunscreen oil may comprise one or more of the following: 4-tert-butyl-4'-methoxybenzoylmethane (“avobenzone”), 2-methylbenzoylmethane, 4-methyl-dibenzoyl-ethane, 4-isopropylbenzoylmethane, 4-tert-butylbenzoylmethane, 2,4-dimethylbenzoylmethane, 2,5-dimethylbenzoylmethane, 4,4'-diisopropylbenzoylmethane, etc. The UV-A sunscreen oil in the personal care composition may preferably be from about 0.5% by weight to about 7% by weight, more preferably from about 1% by weight to about 5% by weight.

[0069] The compositions of any embodiment described herein preferably contain a skin-lightening ingredient. Illustrative skin-lightening ingredients include, but are not limited to, placental extracts, lactic acid, niacinamide, arbutin, kojic acid, ferulic acid, hydroquinone, resorcinol, resorcinol derivatives (including 4-substituted resorcinols, such as, in particular, 4-hexylresorcinol, 4-ethylresorcinol, 4-butylresorcinol and / or 4-isopropylresorcinol), dicarboxylic acids, 12-hydroxystearic acid (“12HSA”), and any combination of two or more thereof. The skin-lightening ingredient preferably includes a tyrosinase inhibitor to complement the melanin-inhibiting activity of a substituted monoamine, such as kojic acid, hydroquinone, and 4-substituted resorcinol. Dicarboxylic acid skin-lightening ingredients include, for example, azelaic acid, sebacic acid, and oxalic acid.

[0070] The amount of the brightening ingredient can be from about 0.1% by weight to about 10% by weight, or both of these values ​​and any range between them.

[0071] Antifungal agents suitable for inclusion in personal care compositions are well known to those skilled in the art. Examples include, but are not limited to, climbazole, ketoconazole, fluconazole, clotrimazole, miconazole, econazole, etaconazole, terbinafine, any one or more salts of these (e.g., hydrochloride), zinc pyrithione, selenium disulfide, octopirox, and any combination of two or more of these. The amount of such substances may be from about 0.000001% by weight to about 10% by weight of the personal care composition.

[0072] Personal care compositions may further comprise from about 0.1% to about 8% by weight of a film-forming polymer. Such film-forming polymers include, but are not limited to, polyalkylene oxide-terminated polyamides (e.g., INCI names: polyamide-3, polyamide-4), polyether polyamides (e.g., INCI name: polyamide-6), mixed acid-terminated polyamides (e.g., INCI name: polyamide-7), and ester-terminated poly(ester-amide) (e.g., INCI name: polyamide-8). Such film-forming polymers may be synthetic or commercially available, such as Sylvaclear from Arizona Chemical Company, LLC. TM Products under this series and Croda International PLC's OleoCraft TM Products within this series. Film-forming polymers also include, but are not limited to, INCI-named polyester-5 (e.g., Eastman AQ). TM 38S Polymer), PPG-17 / IPDI / DMPA copolymer (e.g., Avalure) TM UR450 Polymer), acrylate copolymers (e.g., Avalure) TM AC 120 Polymer) and polysaccharides such as Xilogel (tamarin gum), lotus bean gum, tara gum, β-glucan, pullulan, carboxymethyl cellulose, hydroxypropyl cellulose, sodium alginate, potato starch, carrageenan.

[0073] Other ingredients available in the skin care compositions described herein may be selected from any and all of the following: skin conditioning agents, skin-feeling mildeners, suspending agents, auxiliary thickeners, viscosity control agents, dispersants, solubilizers / clarifiers, stabilizers, opacifiers / pearlizers, chelating agents / sequestering agents, water-soluble additives, bactericides / fungicides, antioxidants, pH control agents, buffers, colorants and fragrances / aromatics, water, and other optional ingredients (additives). The compositions of the present invention may also optionally be incorporated into a water-insoluble matrix for application to the skin, for example, in the form of a treated wipe. According to some embodiments of the invention, preservatives may be incorporated into the personal care compositions to prevent the growth of potentially harmful microorganisms. According to some embodiments of the invention, suitable preservatives for personal care compositions include, but are not limited to, alkyl esters of p-hydroxybenzoic acid. Other suitable preservatives include hydantoin derivatives, propionates, and various quaternary ammonium compounds. Other suitable preservatives include 1,2-alkanediol (e.g., 1,2-octanediol), phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea, sodium dehydroacetate, and benzyl alcohol.

[0074] The composition may contain colorants, opacifiers, or abrasives at concentrations ranging from 0.05% to 5% by weight, or alternatively from 0.1% to 3% by weight, based on the weight of the composition. In some embodiments, the personal care products of the present invention may also contain peptides, such as commercially available pentapeptide derivatives—Matrixyl. TM It is commercially available from Sederma, France. In another instance, in some embodiments, the personal care product of the present invention may also contain carnosine.

[0075] Preferably, the composition is formulated as a powder, flake, emulsion, cream, gel, or mousse. More preferably, the composition is formulated as a cream or emulsion, and most preferably as a cream. The composition is preferably a no-rinse type. The composition of the present invention can be packaged as a patch, bottle, tube, ball applicator, propellant-driven aerosol device, squeeze container, or can with a lid.

[0076] The invention will now be explained with the aid of non-limiting embodiments.

[0077] Example

[0078] The role of trehalose in inhibiting microorganisms such as Staphylococcus aureus and Malassezia:

[0079] The experiment was conducted using the following procedure:

[0080] Experimental steps:

[0081] Step 1: Microbial culture and preparation

[0082] According to the culture conditions shown in Table 1, Staphylococcus hominis (ATCC 27844), Propionibacterium acnes (ATCC 6919), Staphylococcus aureus (ATCC 6538), and Malassezia restricta (ATCC 7877) from the cryopreservation were inoculated onto agar plates. Then, a loopful of microorganisms from the agar plates was inoculated into a liquid culture system according to the culture conditions shown in Table 1. Before use, Malassezia restricta was inoculated twice more onto MLNA plates. A microbial suspension was then prepared using the appropriate liquid broth as shown in Table 1. Based on the absorption at 600 nm, the cell density of the bacteria (Staphylococcus hominis, Propionibacterium acnes, and Staphylococcus aureus) was adjusted to 10-1. 8 A concentration of CFU / mL was determined for further use. Based on the absorbance at 610 nm, the cell density of *Malassezia repens* was adjusted to 10-1. 8 The concentration of cfu / ml is specified for further use. The detailed formula for the broth is listed in Table 2.

[0083] Table 1

[0084]

[0085] Table 2

[0086]

[0087] Step 2: Overlap Analysis

[0088] On day 0, it will contain (1) 2% trehalose and 10 6 cfu / ml human staphylococcus, (2) 10 6 15 ml of TSA containing (3) 2% MLNA / TSA alone (containing no trehalose) and (4) 2% trehalose alone (containing no MLNA / TSA) was solidified in a 90 cm culture dish and incubated at 37°C for 4 days. A parallel TSA agar containing neither MLNA / TSA nor trehalose was prepared as a control. At the end of day 4, 5 ml of MLNA / TSA was applied to the TSA agar, and after solidification, 2 μL of serially diluted target microorganisms (10 μL / ml) were added. 8 -10 1 CFU / mL of restricted Malassezia / Staphylococcus aureus was spotted onto an MLNA / TSA plate. The plate was then further cultured under the conditions shown in Table 1 to monitor the growth of the target microorganism.

[0089] Finally, the Log values ​​of the target microorganisms Malassezia and Staphylococcus aureus on plates (1), (2), and (3) were determined compared with the growth of their respective restriction Malassezia or Staphylococcus aureus on control plates. 10 Reduce value (kill).

[0090] Table 3 Log of Malassezia restricta 10 Reduce value (kill)

[0091] Example number microorganism <![CDATA[Log 10 Reduced value (killed)]]> 1 (2% trehalose + Staphylococcus aureus) Restrictive Malassezia 5 A (2% trehalose alone, Staphylococcus aureus-free) Restrictive Malassezia 0 B (Staphylococcus aureus alone, without trehalose) Restrictive Malassezia 1

[0092] Table 4. Log of Staphylococcus aureus 10 Reduce value (kill)

[0093] Example number microorganism <![CDATA[Log 10 Decrease value (kill) 2 (2% trehalose + Staphylococcus aureus) Staphylococcus aureus 5 C (2% trehalose alone, Staphylococcus aureus-free) Staphylococcus aureus 1 D (Staphylococcus aureus alone, without trehalose) Staphylococcus aureus 2

[0094] As can be clearly seen from Tables 3 and 4 above, trehalose is effective against pathogenic microorganisms such as Malassezia and Staphylococcus aureus in the presence of Staphylococcus aureus.

[0095] Therefore, it is clear from the above description that when applied to the external surface of the human body, trehalose can be effectively used as a prebiotic to inhibit pathogenic microorganisms.

Claims

1. Non-therapeutic use of trehalose, wherein the trehalose, when applied to the external surface of the human body, acts as a prebiotic to inhibit pathogenic microorganisms in the presence of Staphylococcus aureus, wherein the microorganisms are Malassezia restricta and / or Staphylococcus aureus.

2. The use according to claim 1, wherein, The microorganism is Malassezia, and the intended use is to suppress dandruff to promote scalp health.

3. The use according to claim 1 or 2, wherein the outer surface comprises keratinocytes.

4. The use according to claim 1, wherein the outer surface is human skin.

5. The use according to claim 1, wherein the outer surface is the scalp.

6. Non-therapeutic use of trehalose, wherein the trehalose, when applied to the human scalp, inhibits Malassezia restriction in the presence of Staphylococcus aureus.

7. Non-therapeutic use of trehalose, wherein the trehalose is used as a prebiotic in the presence of Staphylococcus aureus to suppress dandruff.

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