A siRNA delivery system and its preparation method and application
CN115869287BActive Publication Date: 2025-09-02苏州唯思尔康科技有限公司
Patent Information
- Application Number
- CN202211461825.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-11-22
- Publication Date
- 2025-09-02
- Estimated Expiration
- 2042-11-22
AI Technical Summary
Technical Problem
In the prior art, the exosomes have small amounts of siRNA loading and low loading efficiency, which makes it difficult to achieve functional delivery of siRNA to target cells and has low preparation efficiency.
Method used
A specific incubation method of liposome Lipo3000 with siRNA and exosomes was used to form a complex and fuse it with exosome vesicles. After incubation, purification was performed to prepare a siRNA delivery system loaded with multiple siRNAs in a single exosome.
Benefits of technology
The number of siRNAs in a single exosome and the proportion of effective exosomes are significantly improved, the functional delivery of siRNAs to target cells is achieved, and the preparation efficiency is improved.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN115869287B_ABST
Abstract
The present invention relates to a siRNA delivery system and its preparation method and application. The siRNA delivery system of the present invention includes exosomes as carriers and a large number of siRNAs loaded in the exosome vesicles. The first composition containing liposomes Lipo3000 and a first culture medium and the second composition containing siRNA and a second culture medium are mixed, and the exosomes are incubated with the exosomes to obtain exosomes loaded with siRNA. The siRNA delivery system of the present invention can be better endocytosed by cells and can achieve functional delivery of siRNA to target cells. The preparation method of the present invention can efficiently load siRNA into exosomes, and the proportion of effective exosomes is high. On the one hand, it ensures that the siRNA delivery system has a good cell-targeted delivery effect and achieves functional delivery of siRNA to target cells. On the other hand, the method has high preparation efficiency, controllable cost, and is suitable for promotion and application.
Need to check novelty before this filing date? Find Prior Art
Citation Information
Patent Citations
Brain glioma targeting system based on magnetic nanoparticle composite engineered exosome and application of brain glioma targeting system
CN114712526A
An in vitro method for obtaining a biohybrid vector
EP3292861A1