A culture solution suitable for cultivating Guizhou local high-quality special rice red rice and / or red rice rice, and its application and rice cultivation method

Abstract

The present invention relates to the technical field of rice planting methods, and specifically to a culture solution suitable for cultivating Guizhou local high-quality special rice red rice and / or red rice rice, as well as an application and a rice cultivation method. In the process of studying the large-scale planting method of "red rice" and "red rice rice", the inventor tried to adjust the proportion of ammonia nitrogen and nitrate nitrogen in the nitrogen source of ordinary rice nutrient solution, add exogenous amino acids and exogenous GABA. It was found that for these two special rices, only adding a certain amount of glycine can achieve the most ideal effect of increasing their plant height, biomass, nitrogen content and chlorophyll content. In addition, for "red rice rice", if exogenous serine, threonine, glycine, proline, alanine and GABA can be added at the same time, the growth of rice seedling roots can be promoted to a greater extent. The use of this technical solution can effectively improve the problem of poor growth and development caused by its inability to adapt to ordinary rice nutrient solution, and is used to improve the environmental adaptability of rice in the field.

Description

Technical Field

[0001] The present invention relates to the technical field of rice planting methods, and in particular to a culture solution suitable for cultivating Guizhou local high-quality special rice red rice and / or red rice rice, as well as an application and a rice cultivation method. Background Art

[0002] Guizhou's local high-quality specialty rice "Xianghenuo" is a general term for glutinous grasses in the Dong ethnic group in Qiandongnan, Guizhou. It is mainly distributed in Liping, Congjiang and Rongjiang counties in southeast Guizhou. It has strong regional characteristics and is highly adaptable to the local natural ecology, such as shade tolerance and cold tolerance (Chen Huicha, Zhang Zaixing, Ruan Renchao, et al. Identification and evaluation of cold tolerance and drought resistance of Guizhou rice and grass germplasm resources [J]. Guizhou Agricultural Science, 1999, 27 (6): 38-40). Today, there are still rich and diverse varieties in the Dong ethnic group area in Qiandongnan, which is one of the valuable genetic resources for rice research in my country. It has excellent stickiness, high quality, and high nutritional value. It does not go stale even when it is cold. Because its rice is fragrant and delicious, it is known as "one family cooks rice, ten families smell it, and one village cooks rice, which smells fragrant for ten miles". It is deeply loved by the Dong people, so it is called "fragrant rice" (Yang Li, Zhou Dingsheng, Zheng Guiyun, et al. Original Ecological Farming Culture in Southeast Guizhou - Rice [J]. Guizhou Agricultural Science, 2008, 36 (4): 23-26). In addition, the trace elements necessary for the human body have many important physiological functions. Eating original ecological green high-quality organic agricultural products and taking in an appropriate amount of trace elements has become a green and environmentally friendly diet for people. Studies have shown that "Xianghe glutinous rice" is rich in trace elements (Tang Wenhua, Jiang Tianzhi, Yang Xiaoyan, et al. Research on the content of Zn and Cr in original Danzhai selenium rice and its physiological function [J]. Anhui Agricultural Science, 2009, 37(32): 15686-15687.), so eating "Xianghe glutinous rice" can not only adjust the taste and increase appetite, but also supplement the appropriate amount of trace elements.

[0003] Among them, "Honghe" and "Hongmihe" are high-quality red rice varieties in "Xianghe Glutinous Rice". They not only have excellent quality but are also rich in anthocyanins. Anthocyanins are a type of water-soluble natural pigment of the flavonoid class and are the main coloring pigments for plants to present red, purple and blue colors. Studies have shown that it has multiple physiological functions such as anti-oxidation, anti-mutation, anti-cancer, prevention of cardiovascular and cerebrovascular diseases, protection of the liver, inhibition of tumor cell occurrence, and has an auxiliary therapeutic effect on chronic degenerative diseases caused by oxidative free radicals. The development and utilization of "Xianghe Glutinous Rice" represented by "Honghe" and "Hongmihe" can meet people's pursuit of high-quality rice and healthy life, and can also protect local high-quality germplasm resources.

[0004] Although "Honghe" and "Hongmihe" have the above-mentioned excellent properties, nowadays a large number of rural laborers have been transferred to cities and towns, and the problem of aging rural population is serious, resulting in a serious shortage of labor. More and more fertile land is uncultivated, resulting in the planting area of ​​"Honghe" and "Hongmihe" and other "fragrant glutinous rice" not increasing but decreasing, and the current situation is quite serious. It is imperative to realize the factory-based seedling cultivation process of "Honghe" and "Hongmihe" to achieve large-scale and intensive planting to quickly expand the planting area. However, due to the strong regionality of "Honghe" and "Hongmihe" and the generally low yield, at the same time, it has been found through experiments that they grow poorly and have poor resistance under ordinary rice nutrient solution culture. These problems have greatly hindered the large-scale and intensive process of "Honghe" and "Hongmihe", making it difficult to expand the planting area. Therefore, exploring a nutrient solution formula and cultivation method suitable for cultivating Guizhou's local high-quality specialty rice "Honghe" and "Hongmihe" is an urgent problem to be solved in order to promote the factory-based seedling cultivation of "Honghe" and "Hongmihe", realize large-scale and intensive planting management and promote planting. Summary of the invention

[0005] The present invention aims to provide a culture solution suitable for cultivating Guizhou local high-quality specialty rice red rice and / or red rice rice, so as to solve the technical problem of lack of effective culture method for "red rice" and "red rice rice" in the prior art.

[0006] In order to achieve the above object, the present invention adopts the following technical scheme:

[0007] The invention discloses a culture solution suitable for culturing red rice and / or red rice grass, which is a common rice nutrient solution added with glycine.

[0008] The present invention also provides an application of a culture solution in culturing Guizhou local high-quality specialty rice red rice and / or red rice rice.

[0009] The present invention also provides a method for cultivating Guizhou local high-quality special rice red rice and / or red rice rice, comprising the following steps performed in sequence:

[0010] S1 rice seeds are soaked, cleaned, and cultured in water until the buds grow to 1-2 cm to obtain germinated seeds;

[0011] S2: culturing the germinated seeds in water until the first incomplete leaf appears; replacing the water with ordinary rice nutrient solution, culturing until the three-leaf stage, and obtaining three-leaf rice seedlings;

[0012] S3 uses the first culture solution or the second culture solution to culture rice seedlings from the three-leaf stage to the five-leaf stage; the first culture solution is a common rice nutrient solution added with glycine; the second culture solution is a common rice nutrient solution added with a neutral amino acid combination and GABA.

[0013] The principle and beneficial effects of this technical solution are:

[0014] Plants obtain nitrogen by absorbing ammonia, nitrate, amino acids, soluble peptides, etc. in the soil. The addition of amino acids provides a more direct material basis for the growth and development of rice than inorganic nitrogen sources. Amino acids are an important form of organic nitrogen in the soil and plants, affecting all processes of plant growth and development. The inventors found that "Honghe" and "Hongmihe" can significantly increase their plant height, biomass and nitrogen content after adjusting the proportion of ammonia nitrogen and nitrate nitrogen in the nitrogen source of ordinary rice nutrient solution and adding exogenous serine, threonine, glycine, proline, and alanine amino acids, thereby effectively improving the problem of poor growth and development caused by their inability to adapt to ordinary rice nutrient solution, and their use in the field may improve their adaptability. The inventors then adjusted the ratio of ammonia nitrogen and nitrate nitrogen in the nitrogen source of ordinary rice nutrient solution and added exogenous organic nitrogen, forming 13 treatments. At the same time, 5 kinds of amino acids were separately added to the ordinary rice nutrient solution to form 5 treatments, a total of 18 treatments, with the aim of screening out the optimal treatment conditions. Finally, it was found that adding exogenous glycine to the ordinary nutrient solution can promote the growth and development of "red grass" and "red rice grass" to the greatest extent. Utilizing the above-mentioned discovery, glycine was added to ordinary rice nutrient solution to cultivate "Honghe" and "Hongmihe", and a nutrient solution suitable for cultivating Guizhou's local high-quality specialty rice "Honghe" and "Hongmihe" was prepared. According to the growth characteristics of "Honghe" and "Hongmihe", a cultivation method based on the nutrient solution formula was proposed. This can solve the problem that "Honghe" and "Hongmihe" grow poorly in ordinary nutrient solution, which makes it difficult to cultivate seedlings in factories and thus cannot be managed and promoted on a large scale and intensively. To a certain extent, it enhances the adaptability of "Honghe" and "Hongmihe" and achieves a small increase in yield, which will be conducive to promoting the process of building green agriculture.

[0015] Among them, the "Xianghe Nuo" described in this scheme is a high-quality specialty rice in Guizhou, which has been reported in many existing technical documents. In addition to the documents mentioned in the background technology, there are: "Zhou Jiangbo, Wu Shuyi, Chen Mi, Lei Qiyi. Guizhou Dong Nationality Xianghe Nuo and its evolution [J]. China Rice, 2019, 25(04): 70-73.", "Yang Weibing. From glutinous rice to indica: a historical investigation on the cultivation of grain crops and ethnic habitat adaptation in Southeast Guizhou [J]. Chinese Agricultural History, 2004, 23(4): 88-96" and so on.

[0016] Furthermore, the concentration of glycine is 0.1 to 0.5 mM.

[0017] Furthermore, in the common rice nutrient solution, NH4 + and NO3 + The molar ratio is 1:1, and the sum of the concentrations is 2.5 mM.

[0018] Furthermore, the elements contained in the common rice nutrient solution also include phosphorus, potassium, calcium, magnesium, manganese, molybdenum, boron, zinc, copper and iron.

[0019] Furthermore, a culture medium suitable for cultivating Guizhou local high-quality specialty rice red rice and / or red rice rice also includes ordinary rice nutrient solution added with a neutral amino acid combination and GABA.

[0020] Furthermore, the neutral amino acid combination consists of serine, threonine, glycine, alanine and proline.

[0021] Furthermore, the final concentrations of serine, threonine, glycine, alanine and proline were 0.1 mM, 0.2 mM, 0.2 mM, 0.3 mM and 0.2 mM, respectively; and the final concentration of GABA was 0.5 mM.

[0022] Furthermore, the rice seeds are seeds of red grass or red rice grass;

[0023] The common rice nutrient solution contains 2.5 mM NH4 + and 2.5 mM NO3 + ; The final concentration of glycine is 0.1-0.5 mM; the neutral amino acid combination consists of serine with a final concentration of 0.1 mM, threonine with a final concentration of 0.2 mM, glycine with a final concentration of 0.2 mM, alanine with a final concentration of 0.3 mM and proline with a final concentration of 0.2 mM; the final concentration of GABA is 0.5 mM.

[0024] In summary, the beneficial effects of this technical solution are:

[0025] (1) The effect of glycine addition on plant height, fresh weight, root length and chlorophyll content of “Red Grass” and “Red Rice Grass”

[0026] "Honghe" and "Hongmihe" are red rice germplasm resources of "Xianghenuo", a local high-quality specialty rice in Guizhou. They have strong resistance and excellent nutritional quality. They are also rich in anthocyanins essential to the human body. Both their appearance and connotation are very consistent with people's pursuit of high-quality nutritious rice today. However, due to their strong regionality and generally low yield, they can only be planted in the southeastern Guizhou region, and many studies on them have been limited to the fields. Now, research has found that adding 0.1-0.5mM glycine to the ordinary nutrient solution of rice can improve the problem of poor growth and development of "Honghe" and "Hongmihe" in ordinary nutrient solution, which provides the possibility of large-scale, intensive planting and large-scale promotion of planting of "Honghe" and "Hongmihe", and also provides the possibility of bringing high-quality red rice to the public's table.

[0027] In the previous research, the inventor tried to apply a variety of nitrogen sources to "Honghe" and "Hongmihe". The inventor tried to combine the application of amino acids, the application of GABA, and the adjustment of the ratio of nitrate nitrogen and ammonium nitrogen, in order to find the best way to apply nitrogen sources. After a lot of attempts, it was found that the exogenous addition of 0.1-0.5mM glycine on the basis of ordinary rice nutrient solution has a very significant promoting effect on the plant height, fresh weight, root length and chlorophyll content of "Honghe" and "Hongmihe". In addition, in the early stage of the experiment, the inventor also tried the solution of adding complex amino acids (t5 of Example 1). The experimental results showed that the combination of 5 neutral amino acids can only play a small role in improving the fresh weight and plant height of the two types of rice plants ( Figure 1 However, when glycine was used alone, its effect on increasing fresh weight and plant height of both rice varieties was greatly enhanced ( Fig. 9 ). This shows that other types of amino acids, except glycine, inhibit the growth-promoting effect of glycine. The traditional view is that applying more types of amino acids should have a stronger promoting effect on plant growth, but the experimental results show that using glycine alone produces unexpected technical effects.

[0028] Moreover, the effect achieved by adding only one amino acid to the ordinary rice nutrient solution is significantly better than the treatment of adding multiple amino acids at the same time and adjusting the ratio of ammonia nitrogen to nitrate nitrogen. Therefore, a nutrient solution formula and cultivation method that are most suitable for the growth and development of "Honghe" and "Hongmihe" were obtained. This formula is simple and efficient in actual production operations, easier to prepare and can take effect more quickly. It will greatly promote the factory seedling cultivation process of these two red rice varieties in "Xianghe Nuo" and help my country's green ecological agriculture construction.

[0029] (2) The combined effects of neutral amino acids and GABA on promoting the growth of plant height, fresh weight and root length of "Red Rice Grass"

[0030] For "Hongmihe", when glycine is used alone, although it has a growth-promoting effect on roots, the intensity is not particularly high. If higher requirements are placed on root length during the planting process, the C2 treatment method can be used, namely: ordinary nutrient solution plus neutral 1 amino acid [Ser (0.1mM), Thr (0.2mM), Gly (0.2mM), Ala (0.3mM), Pro (0.2mM)] and 0.5mM GABA. The joint use of neutral 1 amino acid and GABA produces a synergistic effect in "Hongmihe". The t5 treatment and t9 treatment did not significantly increase the fresh weight and plant height of "Hongmihe" ( Figure 1), indicating that these two treatments had no promoting effect on the growth of "Red Rice Grass". However, after the two treatments were used together (C2), the fresh weight of "Red Rice Grass" increased by more than twice that of the control group C1, and the plant height and root length were promoted at the same time. Therefore, the combined use of the two treatments can form a synergistic effect and obtain unexpected technical effects.

[0031] Therefore, the present invention provides a nutrient solution formula and a cultivation method suitable for the growth and development of "Honghe" and "Hongmihe", which can hopefully solve the problem that "Honghe" and "Hongmihe" are difficult to plant in other places due to their strong regionality and cannot adapt to the hydroponic planting of ordinary rice nutrient solution. It will be beneficial to accelerate the factory-based seedling cultivation process of "Honghe" and "Hongmihe", and realize the large-scale and intensive planting management of "Honghe" and "Hongmihe" as well as large-scale promotion of planting. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The plant height and fresh weight statistics and phenotypic pictures of "Honghe" and "Hongmihe" under different treatment conditions in the organic nitrogen treatment group of Example 1 of the present invention are shown (Duncan test was used to perform a significance analysis, with lowercase letters marked above the data. In the same statistical graph, different lowercase letters indicate significant differences, p < 0.05; the same lowercase letters indicate consistent significance levels).

[0033] Figure 2 The plant height and fresh weight statistics and phenotypic pictures of "Red Grass" and "Red Rice Grass" under different treatment conditions in the inorganic nitrogen treatment group of Example 1 of the present invention (the same as the statistical significance analysis of the data Figure 1 ).

[0034] Figure 3 This is a bar graph comparing the differences in nitrogen content between the "red grass" and "red rice grass" treatments of Example 2 of the present invention (data statistical significance analysis is the same as Figure 1 ).

[0035] Figure 4 The plant height, fresh weight and root length statistics and phenotypic pictures of the "Red Grass" plants under different treatment conditions in the double treatment group of Example 3 of the present invention (the same as the statistical significance analysis of the data Figure 1 ).

[0036] Figure 5 The plant height, fresh weight and root length statistics and phenotypic pictures of the "Red Rice Grass" plant under different treatment conditions in the double treatment group of Example 3 of the present invention (the same as the statistical significance analysis of the data Figure 1 ).

[0037] Figure 6The plant height, fresh weight and root length statistics and phenotypic pictures of "Red Grass" under different treatment conditions in the multi-treatment groups of Example 3 of the present invention (the same as the statistical significance analysis of the data Figure 1 ).

[0038] Figure 7 The plant height, fresh weight and root length statistics and phenotypic pictures of "Red Rice Grass" under different treatment conditions in the multi-treatment group of Example 3 of the present invention (data statistical significance analysis is the same as Figure 1 ).

[0039] Figure 8 The statistical results of plant height, fresh weight and root length of "Red Grass" under different treatment conditions in the single treatment group of Example 3 of the present invention and the phenotypic pictures (the statistical significance analysis of the data is the same as Figure 1 ).

[0040] Fig. 9 The plant height, fresh weight and root length statistics and phenotypic pictures of the "Red Rice Grass" plant under different treatment conditions in the single treatment group of Example 3 of the present invention (the same as the statistical significance analysis of the data Figure 1 ).

[0041] Fig.10 These are the chlorophyll content test results of the best growth and development treatments of "Honghe" and "Hongmihe" according to Example 4 of the present invention and the control (**, p<0.01; *, p<0.05).

[0042] Fig.11 The plant height, fresh weight and root length statistics and phenotypic pictures of the "Zaibian Xiaoxianghe" of comparative example 1 of the present invention (the same as the statistical significance analysis of the data Figure 1 ). DETAILED DESCRIPTION

[0043] The present invention is described below by specific embodiments. Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. In addition, the embodiments should be understood as illustrative rather than limiting the scope of the present invention. The essence and protection scope of the present invention are mainly limited by the claims. For those skilled in the art, without departing from the essence and scope of the present invention, various changes or modifications of the material composition and dosage in these embodiments also belong to the protection scope of the present invention.

[0044] Example 1: Effects of different nitrogen treatments on the growth and development of "Red Grass" and "Red Rice Grass".

[0045] Rice varieties: The "Honghe" and "Hongmihe" involved in this scheme are high-quality red rice varieties in "Xianghe Nuo", which not only have excellent quality but are also rich in anthocyanins. The rice seeds used in the subsequent experiments are the seeds of the two red rice varieties "Honghe" and "Hongmihe" in Guizhou's local high-quality specialty rice "Xianghe Nuo" (the seeds were collected in Congjiang County, Qiandongnan Prefecture, Guizhou Province, and stored in the Plant Hormone and Nutrition Molecular Regulation Laboratory of the Rice Industry Technology Research Institute of Guizhou University). The growth period of Honghe is 159 days, and its seeds are similar to japonica rice, that is, the seeds are short and round, the shell is slightly reddish, and some seeds have short awns. The growth period of Hongmihe is 169 days. Its seeds are similar to indica rice, that is, the seeds are flat and long, and have long awns, with an average length of about 4 cm, and white lines on the seed shell. The seeds of the two red rice varieties "Honghe" and "Hongmihe" were collected in Congjiang County, Qiandongnan Prefecture, Guizhou Province, and stored in the Plant Hormone and Nutrition Molecular Regulation Laboratory of the Rice Industry Technology Research Institute of Guizhou University. The applicant undertakes to release the above two types of rice genetic resources to the public within twenty years from the date of application.

[0046] Rice seed soaking: Soak the rice seeds in 0.6% dilute nitric acid for 6 to 8 hours.

[0047] Cultivation of rice seeds: After soaking, rinse the seeds with deionized water for 3 to 5 times, divide them into culture dishes, add appropriate amount of purified water, culture them in an incubator at 37°C, and change the water 1 to 2 times a day.

[0048] Hydroponics: After the seeds germinate, wait until their buds grow to 1-2 cm, sow them in a 96-well plate and place them in a 4L culture box with clean water for culture until the first incomplete leaf appears. Then change to ordinary rice nutrient solution and culture to the 3-leaf stage, and then transplant them into a 15L culture pot. Add 12L of ordinary rice nutrient solution to the culture pots respectively, set up 19 culture pots, set 9 culture pots as organic nitrogen treatment group, 9 culture pots as inorganic nitrogen treatment group, and one pot as normal nitrogen control group without treatment.

[0049] The preparation of ordinary culture solution is a conventional technical means for those skilled in the art, and the reference method is prepared (Yoshida S, Fomo DA, Cock JH, et al. Routine procedure for growing rice plants in culture solution. In: Laboratory manual for physio-logical studies of rice [J]. International Rice Research Institute, 61-66.). The formula of ordinary rice nutrient solution is shown in Table 1 (nitrogen source is not shown, i.e. nitrogen-free ordinary rice nutrient solution). After a lot of research, the inventor found that the use of conventional nitrogen-free ordinary rice nutrient solution can meet the needs of rice growth. The ratio of Table 1 can meet the needs of rice growth, which is a specific presentation form of nitrogen-free ordinary rice nutrient solution. However, nitrogen-free ordinary rice nutrient solution is not limited to the situation shown in Table 1, and the element ratio can be adjusted to a certain extent according to the conventional means in the art. The focus of this technical solution is to study the influence of the form of nitrogen source on rice growth.

[0050] Normal nitrogen treatment group: only 12L of ordinary nutrient solution was added, and the nitrogen source was NH4NO3 mother solution (NH4 + :NO3 - 1:1), add 30mL, the final concentration is 2.5mM.

[0051] Organic nitrogen treatment group: After 3 days of hydroponics, the seedlings have basically adapted to the culture pots. In order to reduce the workload and facilitate the subsequent work, 20 amino acids, GABA, NH4NO3, NaNO3 and NH4Cl mother solutions were prepared, and the concentration of the mother solution was 1mM. The corresponding amount of mother solution was added to 12L of ordinary culture solution in 9 culture pots of the organic nitrogen treatment group to prepare the concentrations in Table 2. For example, 2.4 ml of Glu mother solution, 2.4 ml of Asp mother solution, 1.2 ml of Arg mother solution, 1.2 ml of His mother solution, 1.2 ml of Lys mother solution, 1.2 ml of Ser mother solution, 2.4 ml of Thr mother solution, 2.4 ml of Gly mother solution, 3.6 ml of Ala mother solution, 2.4 ml of Pro mother solution, 2.4 ml of Val mother solution, 1.2 ml of Cys mother solution, 1.2 ml of Tyr mother solution, 3.6 ml of Gln mother solution, 3.6 ml of Asn mother solution, 1.2 ml of Met mother solution, 3.6 ml of Phe mother solution, 3.6 ml of Iie mother solution, 2.4 ml of Leu mother solution and 1.2 ml of Trp mother solution are added to the t2 culture basin so that the concentrations of these twenty amino acids in the t2 culture basin correspond to those in the treatment table. Add the same amount of amino acid stock solution to the five culture basins t3, t4, t5, t6, and t7 as to the corresponding amino acid stock solution added to t2, and prepare each to the concentration corresponding to the treatment table. Add 1.2 ml, 6 ml, and 24 ml of GABA stock solution to the three culture basins t8, t9, and t10, respectively, so that the concentrations of GABA in the three culture basins are 0.1 mM, 0.5 mM, and 2 mM, respectively.

[0052] Inorganic nitrogen treatment group: The nitrogen source of the common nutrient solution in the four culture pots T2, T3, T5, and T6 was adjusted from a single NH4NO3 to a mixture of NH4Cl and NaNO3, NH4 + With NO3 - The sum of the concentrations remains unchanged. After adding 12L of nitrogen-free ordinary nutrient solution to the four culture pots T2, T3, T5, and T6, add 1mM NH4Cl and NaNO3 mother solution 40ml, 20ml, 48ml, 12ml, 20ml, 40ml, 12ml, and 48ml respectively, so that the NH4 + and NO3 - The ratios are 2:1, 4:1, 1:2, 1:4, NH4 + and NO3 -The sum of the concentrations remains unchanged. The nitrogen source of the ordinary nutrient solution in the two culture pots T4 and T7 was adjusted from NH4NO3 to NH4Cl and NaNO3, respectively. 12L of nitrogen-free ordinary nutrient solution was added to the two culture pots T4 and T7, and then 30ml of NH4Cl mother solution and NaNO3 mother solution were added, respectively, so that the concentrations of NH4Cl and NaNO3 in the two culture pots T4 and T7 were 2.5mM. 8L of ordinary nutrient solution was added to the T8 culture pot, and then 4L of water was added to dilute it, so that each component of the ordinary nutrient solution was diluted 1.5 times. 12L of nitrogen-free ordinary nutrient solution was added to the two culture pots T9 and T10, respectively, and then 1mM NH4NO3 21ml and 39ml were added to the two culture pots T9 and T10, respectively, so that the concentrations of NH4NO3 in the two culture pots T9 and T10 were 1.75mM and 3.25mM. The methods of organic nitrogen treatment, inorganic nitrogen treatment and normal nitrogen treatment are shown in Table 2.

[0053] Table 1: Common rice nutrient solution formula (nitrogen-free)

[0054]

[0055] Table 2: Initial screening treatment (Note: The concentration in brackets in the table is in mM)

[0056]

[0057]

[0058] Sampling and biomass determination: After all treatments of the two varieties have grown to the 5-leaf stage, the plant height and fresh weight of the rice seedlings of various treatments are counted. One seedling with a plant height close to the average plant height is selected from each treatment of the normal nitrogen control group and the organic nitrogen treatment group and the inorganic nitrogen treatment group, and the seedlings are evenly spread out on a black cloth and connected in a row. The plant phenotype, fresh weight, and plant height statistics of "Honghe" under various treatment conditions in the normal nitrogen control group and the organic nitrogen treatment group are shown in the figure below. Figure 1 As shown in A, 1B, and 1C, the plant phenotype, fresh weight, and plant height of the normal nitrogen control group and the organic nitrogen treatment group of "Hongmihe" under each treatment condition are shown in Figure 1 D, 1E, and 1F; the plant phenotype, fresh weight, and plant height statistics of the normal nitrogen control group and the inorganic nitrogen treatment group of "Honghe" under each treatment condition are shown in Figure 2 As shown in A, 2B, and 2C, the plant phenotype, fresh weight, and plant height of the normal nitrogen control group and the inorganic nitrogen treatment group of "Hongmihe" under each treatment condition are shown in Figure 2 D, 2E, and 2F. Figure 1 middle, Figure 1A and 1D are the phenotypes of single rice seedlings of "Honghe" and "Hongmihe" under different treatment conditions of the organic nitrogen treatment group, respectively. From left to right in the figure, they are the control with ordinary nutrient solution, 20 kinds of amino acids added to the ordinary nutrient solution, acidic amino acids added to the ordinary nutrient solution, alkaline amino acids added to the ordinary nutrient solution, neutral 1 amino acid added to the ordinary nutrient solution, neutral 2 amino acids added to the ordinary nutrient solution, neutral 3 amino acids added to the ordinary nutrient solution, 0.1mM GABA added to the ordinary nutrient solution, 0.5mM GABA added to the ordinary nutrient solution, and rice seedlings after 2mM GABA added to the ordinary nutrient solution; Figure 1 B and 1E are bar graphs of fresh weight of single rice seedlings of "Honghe" and "Hongmihe" under various treatment conditions of the organic nitrogen treatment group, respectively. From left to right in the figure, they are the average fresh weight of rice seedlings after adding ordinary nutrient solution, adding 20 kinds of amino acids to the ordinary nutrient solution, adding acidic amino acids to the ordinary nutrient solution, adding alkaline amino acids to the ordinary nutrient solution, adding neutral 1 amino acid to the ordinary nutrient solution, adding neutral 2 amino acids to the ordinary nutrient solution, adding neutral 3 amino acids to the ordinary nutrient solution, adding 0.1mM GABA to the ordinary nutrient solution, adding 0.5mM GABA to the ordinary nutrient solution, and adding 2mM GABA to the ordinary nutrient solution (mean±SD, n=10); Figure 1 C and 1F are the bar graphs of plant height of single rice seedlings of "Honghe" and "Hongmihe" under various treatment conditions of the organic nitrogen treatment group, respectively. From left to right in the figure, they are the average plant height of rice seedlings after adding ordinary nutrient solution, adding 20 kinds of amino acids to the ordinary nutrient solution, adding acidic amino acids to the ordinary nutrient solution, adding alkaline amino acids to the ordinary nutrient solution, adding neutral 1 amino acid to the ordinary nutrient solution, adding neutral 2 amino acids to the ordinary nutrient solution, adding neutral 3 amino acids to the ordinary nutrient solution, adding 0.1mM GABA to the ordinary nutrient solution, adding 0.5mM GABA to the ordinary nutrient solution, and adding 2mM GABA to the ordinary nutrient solution (mean±SD, n=10); Figure 2 middle, Figure 2A and 2D are the phenotypes of single rice seedlings of "Honghe" and "Hongmihe" under different treatment conditions of the inorganic nitrogen treatment group, respectively. From left to right in the figure, they are the control with ordinary nutrient solution added, ordinary nutrient solution without nitrogen added and NH4Cl and NaNO3 mixed in a ratio of 2:1 as a nitrogen source, ordinary nutrient solution without nitrogen added and NH4Cl and NaNO3 mixed in a ratio of 4:1 as a nitrogen source, ordinary nutrient solution without nitrogen added and NH4Cl as a nitrogen source, ordinary nutrient solution without nitrogen added and NH4Cl and NaNO3 mixed in a ratio of 1:2 as a nitrogen source, ordinary nutrient solution without nitrogen added and NH4Cl and NaNO3 mixed in a ratio of 1:4 as a nitrogen source, ordinary nutrient solution without nitrogen added and NaNO3 as a nitrogen source, ordinary nutrient solution diluted 1.5 times, ordinary nutrient solution added and NH4NO3 concentration reduced (1.75mM), ordinary nutrient solution added and NH4NO3 concentration increased (3.25mM) after treatment of rice seedlings; Figure 2 B and Figure 2 E is a bar graph of the fresh weight of individual rice seedlings of "Honghe" and "Hongmihe" under different treatment conditions in the inorganic nitrogen treatment group. From left to right in the figure, they are the control with ordinary nutrient solution, the ordinary nutrient solution without nitrogen and NH4Cl and NaNO3 mixed in a ratio of 2:1 as the nitrogen source, the ordinary nutrient solution without nitrogen and NH4Cl and NaNO3 mixed in a ratio of 4:1 as the nitrogen source, the ordinary nutrient solution without nitrogen and NH4Cl as the nitrogen source, the ordinary nutrient solution without nitrogen and NH4Cl as the nitrogen source, Bar graph of fresh weight of single rice seedlings under the following treatments: mixing NH4Cl and NaNO3 in a ratio of 1:2 as nitrogen source, adding nitrogen-free ordinary nutrient solution and mixing NH4Cl and NaNO3 in a ratio of 1:4 as nitrogen source, adding nitrogen-free ordinary nutrient solution and using NaNO3 as nitrogen source, ordinary nutrient solution diluted 1.5 times, adding ordinary nutrient solution and reducing NH4NO3 concentration (1.75mM), adding ordinary nutrient solution and increasing NH4NO3 concentration (3.25mM) (mean±SD, n=10); Figure 2 C and Figure 2F is a bar graph of the plant height of individual rice seedlings of "Honghe" and "Hongmihe" under various treatment conditions of the inorganic nitrogen treatment group. From left to right in the figure, they are the control with ordinary nutrient solution, ordinary nutrient solution without nitrogen and a mixture of NH4Cl and NaNO3 in a ratio of 2:1 as a nitrogen source, ordinary nutrient solution without nitrogen and a mixture of NH4Cl and NaNO3 in a ratio of 4:1 as a nitrogen source, ordinary nutrient solution without nitrogen and NH4Cl as a nitrogen source, ordinary nutrient solution without nitrogen and The average plant height of rice seedlings (mean±SD, n=10) after treatments with NH4Cl and NaNO3 mixed in a ratio of 1:2 as the nitrogen source, adding nitrogen-free ordinary nutrient solution and using NH4Cl and NaNO3 mixed in a ratio of 1:4 as the nitrogen source, adding nitrogen-free ordinary nutrient solution and using NaNO3 as the nitrogen source, diluting ordinary nutrient solution 1.5 times, adding ordinary nutrient solution and reducing the NH4NO3 concentration (1.75mM), and adding ordinary nutrient solution and increasing the NH4NO3 concentration (3.25mM).

[0059] Based on the above results, it was found that in the organic nitrogen treatment group, the addition of neutral 1 amino acid to the ordinary nutrient solution had a more significant effect on promoting the growth and development of "Honghe" and "Hongmihe". The addition of different concentrations of GABA to the ordinary rice nutrient solution had different effects on the growth and development of the two varieties. Overall, the best effect was achieved by adding 0.5Mm GABA to the ordinary rice nutrient solution. In the six treatments with the addition of exogenous amino acids, whether adding twenty kinds of amino acids at the same time or dividing them into acidic amino acids, alkaline amino acids, neutral 2 and neutral 3 amino acids, the plant growth and development of "Honghe" and "Hongmihe" showed different degrees of inhibitory effects, especially the neutral 3 amino acid treatment showed a very obvious inhibitory effect on the growth and development of "Honghe" and "Hongmihe"; only the addition of neutral 1 amino acid showed a significant promoting effect on the growth and development of both "Honghe" and "Hongmihe" varieties; in the three treatments with the addition of GABA, the plant height of "Honghe" showed a low-high-low trend with the increase of GABA concentration, such as Figure 1 A. Figure 1 C; Weighing them accordingly revealed that in the organic nitrogen treatment group, the fresh weight of "Red Grass" showed a trend of first increasing and then decreasing with the increase of GABA concentration, such as Figure 1 B. The plant height of "Hongmihe" showed a trend of first increasing and then decreasing as the concentration of GABA increased. Figure 1 D. Figure 1 F; Weighing them accordingly revealed that in the organic nitrogen treatment group, the fresh weight of "Red Grass" also showed the same trend as the increase of GABA concentration, such as Figure 1 E.

[0060] In the inorganic nitrogen treatment group, the treatment with a mixture of NH4Cl and NaNO3 in a ratio of 4:1 in a nitrogen-free ordinary nutrient solution as a nitrogen source had a more significant effect on promoting the growth and development of "Red Grass". The treatment with a mixture of NH4Cl and NaNO3 in a ratio of 1:4 in a nitrogen-free ordinary nutrient solution as a nitrogen source had a more significant effect on promoting the growth and development of "Red Rice Grass". In the eight treatments that only changed the ratio or concentration of ammonia nitrogen and nitrate nitrogen in the common nutrient solution, the treatment of mixing NH4Cl and NaNO3 in a ratio of 4:1 as a nitrogen source had a more obvious promoting effect on the growth and development of "Red Grass", and the other treatments showed different degrees of inhibitory effects on the growth and development of "Red Grass"; the treatment of mixing NH4Cl and NaNO3 in a ratio of 1:4 as a nitrogen source had a more obvious promoting effect on the growth and development of "Red Rice Grass", and the other treatments showed different degrees of inhibitory effects on the growth and development of "Red Rice Grass"; using a single NH4Cl or NaNO3 instead of NH4NO3 as a nitrogen source would have an inhibitory effect on the growth and development of "Red Grass" and "Red Rice Grass", and whether increasing or decreasing the concentration of NH4NO3 in the common nutrient solution would inhibit the growth and development of "Red Grass" and "Red Rice Grass". The overall dilution of the common nutrient solution by 1.5 times also showed an inhibitory effect on the growth and development of "Red Grass" and "Red Rice Grass". Figure 2 A, 2C, 2D, 2F; they were weighed accordingly and found that in the inorganic nitrogen treatment group, the treatments with NH4Cl and NaNO3 mixed in a ratio of 4:1 and 1:4 in the nitrogen-free ordinary nutrient solution had a significant promoting effect on the fresh weight increase of "Honghe" and "Hongmihe". Among the eight treatments that only changed the nitrogen source composition or concentration in the ordinary nutrient solution, the treatments with NH4Cl and NaNO3 in a ratio of 4:1 and 1:4 had a more obvious promoting effect on the fresh weight increase of "Honghe" and "Hongmihe", respectively. Changing this ratio would have an inhibitory effect on the growth and development of "Honghe" and "Hongmihe"; using a single NH4Cl or NaNO3 instead of NH4NO3 as a nitrogen source would have an inhibitory effect on the fresh weight increase of "Honghe" and "Hongmihe", and whether increasing or decreasing the concentration of NH4NO3 in the ordinary nutrient solution would inhibit the fresh weight increase of "Honghe" and "Hongmihe". The overall dilution of the ordinary nutrient solution by 1.5 times also showed an inhibitory effect on the fresh weight increase of "Honghe" and "Hongmihe". Figure 2 B. 2E.

[0061] In summary, the inventors discovered for the first time that after dividing fifteen neutral amino acids into three groups and treating "Honghe" and "Hongmihe", it was found that under the cultivation of one neutral amino acid (serine, threonine, glycine, proline, and alanine), the plant height and fresh weight of these two varieties were significantly higher than those of the control, and the effect of promoting growth and development was very significant; under the cultivation of three neutral amino acids (methionine, phenylalanine, isoleucine, leucine, and tryptophan), the plant height and fresh weight of these two varieties were significantly lower than those of the control, and the effect of inhibiting growth and development was very obvious.

[0062] The inventors first discovered that adjusting the ratio of ammonia nitrogen to nitrate nitrogen and applying exogenous GABA without changing the nitrogen concentration plays an important role in the growth and development of "red grass" and "red rice grass", mainly in terms of promoting plant height and fresh weight. Adjusting the ratio of ammonia nitrogen to nitrate nitrogen under the premise of keeping the nitrogen concentration in the ordinary nutrient solution unchanged can significantly increase the plant height and fresh weight of rice. Adding 0.1-0.5mM GABA to the ordinary nutrient solution can increase the plant height and fresh weight of rice and the total nitrogen content of the plant to a certain extent.

[0063] The above results show that in the organic nitrogen treatment group, exogenous addition of appropriate concentrations of neutral 1 amino acids or 0.5mM GABA on the basis of ordinary rice nutrient solution can promote the growth and development of "Honghe" and "Hongmihe", and improve their poor growth and development problems in ordinary nutrient solution; in the inorganic nitrogen treatment group, mixing NH4Cl and NaNO3 in a ratio of 4:1 as the nitrogen source in the nitrogen-free ordinary nutrient solution is more conducive to the growth and development of "Honghe" than the ordinary nutrient solution, and mixing NH4Cl and NaNO3 in a ratio of 1:4 as the nitrogen-free ordinary nutrient solution is more conducive to the growth and development of "Honghe". The treatment of nitrogen source in the nutrient solution is more conducive to the growth and development of "Red Rice Grass" than ordinary nutrient solution; both the organic nitrogen treatment group and the inorganic nitrogen treatment group have treatments that have a relatively obvious effect on promoting the growth and development of "Red Rice Grass" and "Red Rice Grass", and can be used to improve the problems of poor growth and development and poor resistance of "Red Rice Grass" and "Red Rice Grass" in ordinary nutrient solution. Applying these nutrient solution formulas to actual production can accelerate the process of factory-based seedling cultivation of "Red Rice Grass" and "Red Rice Grass", which is conducive to promoting the large-scale, intensive planting management and large-scale promotion of "Red Rice Grass" and "Red Rice Grass".

[0064] Example 2: Effects of different nitrogen treatments on the total nitrogen content of rice in "Honghe" and "Hongmihe"

[0065] The materials of rice in this example are all from the above-mentioned culture materials. Nitrogen is the mineral element with the largest demand by plants and is also called the "element of life". When nitrogen is sufficient, plants can synthesize more protein, promote cell division and enlargement, plant leaf area grows faster, and photosynthesis is stronger. When nitrogen is severely deficient, the synthesis of organic matter is blocked, the plants are short, the leaves turn yellow, and the old leaves are even yellower. Therefore, the nitrogen content in the plants can be used as a physiological indicator to judge the growth and development of the plants. The method for measuring the nitrogen content in rice plants is carried out with reference to the literature (Edited by Nanjing Agricultural College. Soil Agrochemical Analysis. Beijing: Agricultural Press, 1980.193-197).

[0066] Rice plant collection: From the above-mentioned organic nitrogen treatment group and inorganic nitrogen treatment group, the treatments that had more obvious promotion and inhibition on the growth and development of "Honghe" and "Hongmihe" were selected. In the organic nitrogen group, "Honghe" and "Hongmihe" both selected T5 treatment and T9 treatment with almost obvious promotion effects, and T7 treatment with obvious inhibition effect; in the inorganic nitrogen group, "Honghe" selected T3 treatment with the best promotion effect, and "Hongmihe" selected T6 treatment with the best promotion effect. Together with the control, there were five treatments in total. Three rice plants were taken from each treatment, placed in an 80℃ oven to dry overnight, and then the dried plants were taken out and divided into leaves and stems, and the samples were crushed into powder with a grinder for later use.

[0067] Determination of nitrogen content in plants:

[0068] 1. Digestion:

[0069] 1. Use a 1 / 10,000 balance to weigh 0.5 g of sample (dry sample) and place it in the digestive tube.

[0070] 2. Use a 1:1 balance to weigh 0.5 g of copper sulfate pentahydrate and 4.5 g of potassium sulfate and place them in the digestive tube.

[0071] 3. Use a pipette to accurately draw 8 ml of concentrated sulfuric acid into the digestive tube.

[0072] 4. Turn on the instrument and start digestion. First, digest at 280℃ for about 20 minutes, then change to 350℃ for about 20 minutes, and finally change to 450℃ for digestion. Wait until the liquid in the digestion tube turns into emerald green and digest for another 30-40 minutes. Digestion is complete.

[0073] 5. Allow to cool at room temperature for later use.

[0074] 2. Distillation and titration: The sample solution digested above needs to be diluted to 100ml with distilled water, 5ml of the test solution is drawn into the long glass tube of the nitrogen analyzer, and 5ml of the boric acid indicator solution is drawn into a wide-mouthed triangular flask. After distillation for 3 minutes, remove it. Titrate with a semi-micro burette, and the titration changes from green to white to pink. The obtained data is used to calculate the nitrogen content in the plant according to the following formula.

[0075] Total nitrogen in the plant (%) = equivalent concentration of H2SO4 standard solution × (volume of H2SO4 standard solution consumed when titrating the sample - volume of H2SO4 standard solution in the blank test) × fractionation times × 0.014 × 100 / sample weight

[0076] In the formula: the multiple of taking according to the above method is 20, because 5 ml is taken out of 100 mL, and the sample weight is 0.5 g. Finally, the average nitrogen content of the plants measured by the three repeated experiments for each treatment is calculated.

[0077] The plant nitrogen content data measured for each treatment sample was statistically plotted, and the results were as follows: Figure 3 As shown in the figure, the above results once again show that in the organic nitrogen treatment group, the exogenous application of appropriate concentrations of neutral five amino acids or 0.5mM GABA on the basis of ordinary nutrient solution can promote the growth and development of "Honghe" and "Hongmihe", and improve their poor growth and development in ordinary nutrient solution; in the inorganic nitrogen treatment group, the two treatments of using NH4Cl and NaNO3 mixed in a ratio of 4:1 or 1:4 as nitrogen sources in nitrogen-free ordinary nutrient solution are more conducive to the growth and development of "Honghe" and "Hongmihe" than ordinary nutrient solution, and are more suitable for cultivating "Honghe" and "Hongmihe"; both the organic nitrogen treatment group and the inorganic nitrogen treatment group have treatments that have a relatively obvious effect on the growth and development of "Honghe" and "Hongmihe", and can be used to improve the problem that "Honghe" and "Hongmihe" cannot grow normally in ordinary nutrient solution due to their strong regionality, which is conducive to promoting the large-scale planting management and large-scale promotion of "Honghe" and "Hongmihe".

[0078] Example 3: Effects of superimposed treatment on the growth and development of "Red Grass" and "Red Rice Grass"

[0079] The process of soaking rice seeds, culturing rice seeds and hydroponics is as described in Example 1. In the hydroponics process of this example, 18 culture pots were set up, including 1 pot of control with only ordinary rice nutrient solution added, 7 pots of double treatment groups with two treatments superimposed, 7 pots of multi-treatment groups with three treatments superimposed (C2 only contains two treatments, but for the convenience of comparison and photography, it is classified in the three-treatment group), and 5 pots of single treatment groups with only one amino acid exogenously added on the basis of ordinary rice nutrient solution.

[0080] Normal nitrogen control group: add 12L of rice common nutrient solution, the nitrogen source is NH4NO3, of which NH4 + :NO3 - It is 1:1.

[0081] Double treatment group: Add the corresponding mother solution to the corresponding pots in the two treatment groups to make the corresponding solution concentrations in the above table. After adding 12L of nitrogen-free nutrient solution to the B2 culture pot, add 40ml and 20ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, and 2.4ml of Pro mother solution. After adding 12L of nitrogen-free nutrient solution to the B3 culture pot, add 48ml and 12ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, and 2.4ml of Pro mother solution. After adding 12L of nitrogen-free nutrient solution to the B4 culture pot, add 20ml and 40ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, and 2.4ml of Pro mother solution. After adding 12L of nitrogen-free nutrient solution to the B5 culture pot, add 12ml and 48ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, and 2.4ml of Pro mother solution. After adding 12L of nitrogen-free nutrient solution to the B6 culture pot, add 40ml and 20ml of NH4Cl and NaNO3 mother solutions, and 6ml of GABA mother solution. After adding 12L of nitrogen-free nutrient solution to the B7 culture pot, add 48ml and 12ml of NH4Cl and NaNO3 stock solutions respectively, and 6ml of GABA stock solution.

[0082] Multi-treatment group: Add the corresponding mother solution to the corresponding pots in the multi-treatment group to make the corresponding solution concentration in the above table. After adding 12L of ordinary nutrient solution to the C2 culture pot, add 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, 2.4ml of Pro mother solution, and 6ml of GABA mother solution. After adding 12L of nitrogen-free nutrient solution to the C3 culture pot, add 40ml and 20ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, 2.4ml of Pro mother solution, and 6ml of GABA mother solution. After adding 12L of nitrogen-free nutrient solution to the C4 culture pot, add 48ml and 12ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, 2.4ml of Pro mother solution, and 6ml of GABA mother solution. After adding 12L of nitrogen-free nutrient solution to the C5 culture pot, add 48ml and 12ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, 2.4ml of Pro mother solution, and 1.2ml of GABA mother solution. After adding 12L of nitrogen-free nutrient solution to the C6 culture pot, add 12ml and 48ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, 2.4ml of Pro mother solution, and 6ml of GABA mother solution. After adding 12L of nitrogen-free nutrient solution to the C7 culture pot, add 20ml and 40ml of NH4Cl and NaNO3 mother solutions, 1.2ml of Ser mother solution, 2.4ml of Thr mother solution, 2.4ml of Gly mother solution, 3.6ml of Ala mother solution, 2.4ml of Pro mother solution, and 1.2ml of GABA mother solution.

[0083] Single treatment group: Add the corresponding mother solution to the corresponding pots in the single treatment group to make the corresponding solution concentration in the above table. After adding 12L of ordinary nutrient solution to the D2 culture pot, add 1.2ml of Ser mother solution. After adding 12L of ordinary nutrient solution to the D3 culture pot, add 2.4ml of Thr mother solution. After adding 12L of ordinary nutrient solution to the D4 culture pot, add 2.4ml of Gly mother solution. After adding 12L of ordinary nutrient solution to the D5 culture pot, add 3.6ml of Ala mother solution. After adding 12L of ordinary nutrient solution to the D6 culture pot, add 2.4ml of Pro mother solution.

[0084] See Table 3 for specific grouping.

[0085] Table 3: Compound treatment (the concentration in brackets is in mM)

[0086]

[0087] Sampling and biomass determination: After all treatments have grown to the 5-leaf stage, the plant height, fresh weight and root length of rice seedlings under various treatments were counted. A seedling with a plant height near the average value was randomly selected from each treatment pot of the normal nitrogen control and double treatment groups, and placed on a black cloth and spread evenly in a row. The plant height, fresh weight and root length phenotypes of "Honghe" and "Hongmihe" seedlings under each treatment in the double treatment group were as follows: Figure 4 , 5 The phenotypic statistics of plant height, fresh weight and root length of "Honghe" and "Hongmihe" seedlings under each treatment in the multi-treatment group are shown in Figure 6 , 7 As shown in the figure, the phenotypic statistics of plant height, fresh weight and root length of "Honghe" and "Hongmihe" seedlings under each treatment in the single treatment group are as follows Figure 8 , 9 shown in Figure 4 and Figure 5 middle, Figure 4 A and 5A are the phenotypes of individual rice seedlings of “Honghe” and “Hongmihe” under various treatment conditions of the double treatment group, from left to right they are B1, B2, B3, B4, B5, B6, B7; Figure 4 B and 5B are the bar graphs of the plant height of individual rice seedlings of “Honghe” and “Hongmihe” under each treatment condition of the double treatment group (mean±SD, n=10); Figure 4 C and 5C are the bar graphs of fresh weight of individual rice seedlings of “Honghe” and “Hongmihe” under each treatment condition of the double treatment group (mean±SD, n=10); Figure 4 D and 5D are the bar graphs of root length of single rice seedlings of "Honghe" and "Hongmihe" under each treatment condition of the double treatment group (mean±SD, n=10). Figure 6 and Figure 7 middle, Figure 6 A and 7A are the phenotypes of individual rice seedlings of “Honghe” and “Hongmihe” under various treatment conditions of the multi-treatment group, from left to right they are C1, C2, C3, C4, C5, C6, C7; Figure 6 B and 7B are the bar graphs of plant height of individual rice seedlings of “Honghe” and “Hongmihe” under each treatment condition of the multi-treatment group (mean±SD, n=10); Figure 6 C and 7C are the bar graphs of fresh weight of individual rice seedlings of “Honghe” and “Hongmihe” under various treatment conditions of the multi-treatment groups (mean±SD, n=10); Figure 6 D and 7D are the bar graphs of root length of single rice seedlings of "Honghe" and "Hongmihe" under various treatment conditions in the multi-treatment groups (mean ± SD, n = 10). Figure 8 and Fig. 9 middle, Figure 8 A and 9A are the phenotypes of individual rice seedlings of "Honghe" and "Hongmihe" under various treatment conditions of the multi-treatment group, from left to right they are D1, D2, D3, D4, D5, and D6; Figure 8 B and 9B are the bar graphs of the plant height of individual rice seedlings of “Honghe” and “Hongmihe” under each treatment condition of the single treatment group (mean±SD, n=10); Figure 8 C and 9C are the bar graphs of fresh weight of individual rice seedlings of “Honghe” and “Hongmihe” under each treatment condition of the single treatment group (mean±SD, n=10); Figure 8 D and 9D are the bar graphs of root length of individual rice seedlings of “Honghe” and “Hongmihe” under various treatment conditions of the single treatment group (mean±SD, n=10).

[0088] Based on the above results, the specific analysis is as follows:

[0089] Regarding "Hongmihe":

[0090] In the double treatment group, the most significant effect on the growth and development of "Hongmihe" was achieved in the B7 treatment. Only the plant height of "Hongmihe" cultivated under the B3 and B7 treatments was higher than that of the control. There was no significant difference between the plant height under the B4 treatment and the control. The plant height of "Hongmihe" cultivated under the other treatments was lower than that of the control. Figure 5 A, 5B, and weighed them accordingly. It was found that the fresh weight of "Hongmihe" cultivated in treatments B2, B3, B5, and B7 was greater than that of the control, while the other three pots had no significant difference from the control. Figure 5 C. The root length of "Hongmihe" in each treatment was measured and it was found that the root length of "Hongmihe" in all treatments was shorter than that of the control. Figure 5 D. Therefore, adding amino acids and adjusting the ratio of inorganic nitrogen sources to ordinary rice nutrient solution at the same time, or adding GABA and adjusting the ratio of inorganic nitrogen sources to ordinary rice nutrient solution at the same time, is not very ideal for promoting the growth of "Red Rice Grass". B3 and B7, although the treatment has an effect on improving plant height, the increase is limited; B2, B3, B5, B7 groups, although the treatment has an effect on improving plant fresh weight, the increase is also very limited. In Example 1, we observed that for "Red Rice Grass", applying neutral 1 amino acid [Ser (0.1), Thr (0.2), Gly (0.2), Ala (0.3), Pro (0.2)] (t5) alone; applying GABA (0.5) alone (t9); adjusting NH4 + :NO3 -The ratio of t5 to t6 in Example 1 was 1:4 (T6). The above conditions can significantly promote the growth of "Red Rice Grass". The inventor initially believed that the superposition of the above promotion conditions in pairs can further promote plant growth. However, the experimental results are not so. B5 is the superposition of t5 and T6 in Example 1, and B7 is the superposition of t9 and T6 in Example 1. The experimental results of B5 and B7 did not show that the superposition of the two treatments can produce a synergistic effect.

[0091] In the multi-treatment groups, the plant height and fresh weight of "Hongmihe" in C2 and C3 treatments were higher than those in the control, while the plant height and fresh weight of "Hongmihe" in the other treatments were lower than those in the control. Figure 7 A, 7B, 7C, measuring the root length of "Hongmihe" in each treatment, it was found that only the root length of "Hongmihe" in C2 treatment was longer than that of the control, while the root length of "Hongmihe" in the other treatments was shorter than that of the control. Figure 7 D. Only the C2 treatment group (neutral 1 amino acid + 0.5 mM GABA) showed significant advantages over the control group.

[0092] In the single treatment groups, only the plant height of "Hongmihe" in the D5 treatment was lower than that of the control, while the plant heights of "Hongmihe" in the other treatments were higher than that of the control. Fig. 9 A, 9B, and weighed them accordingly. It was found that the fresh weight of the "Hongmihe" in the three treatments D4, D5, and D6 was greater than that of the control. The fresh weight of the material in the D3 treatment had no significant difference from that of the control. The fresh weight of the "Hongmihe" in the D2 treatment was less than that of the control. Fig. 9 C. The root lengths of "Hongmihe" in each treatment were measured and it was found that only the root length of "Hongmihe" in treatment D4 was longer than that of the control, while the root lengths of "Hongmihe" in the other treatments were shorter than that of the control. Fig. 9 D.

[0093] The treatments of B2-B7 cannot effectively promote the root growth of rice, and the optimal group B7 has no obvious advantages over C2 and D4 in promoting plant height and fresh weight, so the treatments of B2-B7 should not be used. Compared with C2 and D4, there is no obvious difference between the two in promoting plant height and fresh weight, but the treatment method of D4 is simpler and easier to promote. When there is a need to promote plant height and fresh weight, the method of adding glycine (D4) can be considered. If there is a need to further increase root length, the treatment method of C2 can be considered. Compared with D4, C2 is better in promoting root growth. On the basis of promoting plant height and fresh weight, it has a further promoting effect on root length.

[0094] Regarding "Honghe":

[0095] In the double treatment group, the most significant effect on the growth and development of "Red Grass" was achieved in the B3 treatment. The plant height and fresh weight of "Red Grass" cultivated under each treatment in the double treatment group were better than those of the control, while only B3 and B4 were better than the control in terms of root length. Figure 4 A, 4B, 4C, 4D.

[0096] In the multi-treatment groups, the C4 treatment had the most significant effect on promoting the growth and development of various indicators of "Red Grass". In the various treatments of the multi-treatment group, the height of "Red Grass" cultivated in the four treatments of C2, C3, C4, and C5 was higher than that of the control, while the height of the remaining two treatments was lower than that of the control. Figure 6 A, 6B, and weighed them accordingly. It was found that only the fresh weight of "Honghe" in treatments C2, C3, and C4 was greater than that in the control, while the fresh weight of treatment C6 was no different from that in the control. The fresh weight of the other two treatments was less than that in the control. Figure 6 C. The root lengths of the above materials were measured and it was found that only the root length of "Red Grass" in the C2 treatment was longer than that of the control, while the others were shorter than the control. Figure 6 D. That is, using the treatment schemes in the multi-treatment group, no treatment method has been found that can simultaneously promote fresh weight, plant height and root length.

[0097] In the single treatment groups, the most significant effect on the growth and development of "Honghe" was in the D4 treatment, and the most significant effect on the growth and development of "Hongmihe" was in the D4 treatment. In the single treatment groups, the plant height and fresh weight of "Honghe" in the five treatments of D2, D3, D4, D5, and D6 were better than those in the control. Figure 8 A, 8B, 8C, measuring the root length of "Red Grass" in each treatment, it was found that only the root length of "Red Grass" in D2 treatment was shorter than that of the control, while the root length of "Red Grass" in the other treatments was longer than that of the control. Figure 8 D.

[0098] The treatment method of using glycine alone can improve the quality of "Honghe" in all aspects, with significant effects in plant height, fresh weight and root length. Although the treatment method of B3 has a similar effect, the increase is not obvious, and the treatment method is more complicated (it is necessary to adjust the ratio of ammonium nitrogen and nitrate nitrogen to 4:1, and add 5 neutral amino acids Ser, Thr, Gly, Ala, Pro). The comparison between D4 and B3 shows that the addition of other amino acids and the adjustment of the ratio of ammonium nitrogen and nitrate nitrogen will inhibit glycine to a certain extent. The treatment method of D4 (using only glycine) can achieve the purpose more easily and obtain unexpected technical effects.

[0099] In summary, the inventors discovered for the first time that the five neutral amino acids have different important roles in the growth and development of "red grass" and "red rice grass", which are mainly reflected in promoting plant height, fresh weight, root length, etc. Under ordinary nutrient solution culture conditions, the exogenous addition of 0.1mM serine treatment conditions can significantly increase the plant height and fresh weight of "red grass", and have no obvious promoting effect on the growth and development of "red rice grass". Under ordinary nutrient solution culture conditions, the exogenous addition of 0.2mM glycine treatment conditions can significantly increase the plant height, fresh weight, root length and chlorophyll content in the leaves of "red grass" and "red rice grass". Under ordinary nutrient solution culture conditions, the exogenous addition of 0.2mM proline treatment conditions can significantly increase the plant height, fresh weight and root length of "red grass" and "red rice grass", but the overall effect is slightly worse than glycine. Under ordinary nutrient solution culture conditions, the exogenous addition of 0.2mM threonine treatment conditions can significantly increase the plant height, fresh weight and root length of "red grass", and have no obvious promoting effect on the growth and development of red rice grass. Under normal nutrient solution conditions, exogenous addition of 0.3 mM alanine can significantly increase rice plant height and fresh weight, but has no significant promoting effect on the growth and development of red rice grass.

[0100] The above results show that there is more than one nutrient solution formula that can promote the growth and development of red rice and red rice, but after comparison, it was found that the best formula is to add glycine exogenously on the basis of ordinary rice nutrient solution. The inventor further adjusted the concentration range of glycine to 0.1-0.5mM, and conducted tests, and found that within this concentration range, the formula can promote the growth and development of the two types of red rice, solve the problem of poor growth and development in ordinary nutrient solution, not only is it very efficient, but also very simple and easy to prepare, and can be put into use faster and better in actual production, and simply and efficiently improve the poor growth and development of the two red rices, and accelerate the process of factory-based seedling cultivation to expand their planting area.

[0101] Example 4 Effect of exogenous application of glycine on the chlorophyll content in leaves of red grass and red rice grass.

[0102] The rice materials in this example are all from the above-mentioned culture materials, and the chlorophyll content of rice leaves is related to the photosynthetic rate and crop growth rate. In scientific research, chlorophyll content is often measured to characterize the growth status of rice, and in production, leaf color changes are often used as an important indicator for seedling diagnosis and fertilizer and water measures. Therefore, this example mainly explores the effect of exogenous application of glycine on the chlorophyll content of "red grass" and "red rice grass". The method for measuring the chlorophyll content in rice leaves is carried out with reference to the literature (Nurkaimaier Murati, Yang Yajie, Parhat Abdukerimu, et al. Comparison of methods for determining chlorophyll content in wheat [J]. Jiangsu Agricultural Science, 2021, 49(09): 156-159.).

[0103] Rice leaf material: Select three rice seedlings from the D4 treatment of the above single treatment group (with D1 as the control), take the sword leaf part, cut the leaves except the veins into pieces, and mix them evenly. Weigh 0.2-0.3g of leaf fragments, put them in a 15ml centrifuge tube, mark the leaf weight, add 10ml of anhydrous ethanol, repeat the experiment for 3 groups, and take the average value. Wrap all centrifuge tubes containing rice leaf fragments and anhydrous ethanol with tin foil, store in the dark for 3 days, and shake them several times during the period.

[0104] Determination of chlorophyll content: After 3 days, obtain the chlorophyll extracts of all samples. Prepare 4 cuvettes, add 4 ml of anhydrous ethanol to one of them as a control, and add 3 sets of repeated chlorophyll extracts of each treated sample to the other three cuvettes. Determine the absorbance values ​​at 663nm and 645nm respectively. Calculate the chlorophyll content according to the following formula using the obtained data.

[0105] (1) Chlorophyll a content = (12.7D663 nm - 2.69D645 nm) × V / 1000 × m

[0106] (2) Chlorophyll b content = (22.9D645 nm-4.68D663 nm) × V / 1000 × m

[0107] (3) Total chlorophyll = (20.21D645 nm + 8.02D663 nm) × V / 1000 × m

[0108] Where: D663 nm and D645 nm are the absorbance at 663 and 645 nm respectively; V is the volume of the test solution (ml); m is the fresh sample weight of the leaf (g) or the leaf area (cm 2 ). Finally, the total amount of chlorophyll measured by the three repeated experiments for each treatment was averaged.

[0109] The measured chlorophyll content was statistically plotted, and the results were as follows: Fig.10As shown in the figure, after adding 0.2mM glycine to the ordinary nutrient solution of rice, the chlorophyll a and total chlorophyll contents in the leaves of "Honghe" were significantly higher than those in the control, while the chlorophyll b content was lower than that in the control; the chlorophyll a, chlorophyll b and total chlorophyll contents in the leaves of "Hongmihe" were significantly higher than those in the control. As an important indicator of plant nutritional status, the high total chlorophyll content in the leaves represents a stronger photosynthesis potential and can synthesize more nutrients required for plant growth and development. This once again confirms that changing the nutrient solution formula can greatly improve the growth and development of "Honghe" and "Hongmihe" in "Xianghenuo", solve the problem of difficulty in factory-based seedling cultivation due to poor growth and development in ordinary nutrient solution, and accelerate the large-scale and intensive planting and management of these two high-quality red rice varieties against the background of the gradual loss of rural labor.

[0110] Comparative Example 1: Planting experiment on "Zaibian Xiaoxianghe"

[0111] Refer to the method in Example 3 to process "Zaibian Xiaoxianghe", which is a white rice variety in "Xianghenu". The growth period of "Zaibian Xiaoxianghe" involved in this comparative example is 160 days, and the seed shape is similar to that of japonica rice, that is, short and round, with long awns and an average length of about 4 cm. The seeds of Zaibian Xiaoxianghe were collected in Congjiang County, Qiandongnan Prefecture, Guizhou Province, and are now stored in the Plant Hormone and Nutrition Molecular Regulation Laboratory of the Rice Industry Technology Research Institute of Guizhou University. The applicant promises to distribute the above-mentioned rice genetic resources to the public within twenty years from the date of application. The experimental results show that when "Zaibian Xiaoxianghe" was treated under the treatment conditions of D1-D6, it did not show similar Figure 8 and Fig. 9 The addition of glycine did not significantly promote the growth of "Zaibian Xiaoxianghe" (see Fig.11 , mean±SD, n=10). This comparative experiment shows that the effect of glycine is different for different rice varieties, and it does not have a fixed mode of action. For the two varieties of "Honghe" and "Hongmihe", adding glycine alone can obtain better technical effects than other treatments (D1-D3, D5, D6, C1-C7, B1-B7, t1-t10, T1-T10), which was not expected by the inventor before the experiment. Moreover, in the experiment on "Zaibian Xiaoxianghe", the inventor found that the comprehensive effect of D4 treatment (glycine alone) was worse than D6, B4, B3, etc., indicating that the phenomenon that adding glycine alone is better than other treatments is not inevitable. The beneficial effect of D4 treatment (glycine alone) is an unexpected phenomenon discovered in the treatment of "Honghe" and "Hongmihe".

[0112] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A use of a culture solution suitable for culturing red rice and / or red rice grass of Guizhou local high-quality specialty rice in culturing red rice and / or red rice grass, characterized in that: It is a common rice nutrient solution added with glycine, or a common rice nutrient solution added with a neutral amino acid combination and GABA; In the common rice nutrient solution, NH4 + and NO3 + The molar ratio is 1:1, and the sum of the concentrations is 5 mM; The elements contained in the common rice nutrient solution also include phosphorus, potassium, calcium, magnesium, manganese, molybdenum, boron, zinc, copper and iron; For the ordinary rice nutrient solution supplemented with glycine, the concentration of glycine was 0.2 mM; For ordinary rice nutrient solution added with a neutral amino acid combination and GABA, the neutral amino acid combination consists of serine, threonine, glycine, alanine and proline; the final concentrations of serine, threonine, glycine, alanine and proline are 0.1mM, 0.2mM, 0.2mM, 0.3mM and 0.2mM respectively; the final concentration of GABA is 0.5mM.

2. A method for cultivating Guizhou local high-quality specialty rice red rice and / or red rice rice, characterized in that: The method includes the following steps in sequence: S1 rice seeds are soaked, cleaned, and cultured in water until the buds grow to 1-2 cm to obtain germinated seeds; S2: culturing the germinated seeds in water until the first incomplete leaf appears; replacing the water with ordinary rice nutrient solution, culturing until the three-leaf stage, and obtaining three-leaf rice seedlings; S3: using the first culture solution or the second culture solution to culture rice seedlings from the three-leaf stage to the five-leaf stage; the first culture solution is a common rice nutrient solution supplemented with glycine; the second culture solution is a common rice nutrient solution supplemented with a neutral amino acid combination and GABA; The rice seeds are seeds of red rice or red rice; The common rice nutrient solution contains 2.5 mM NH4 + and 2.5 mM NO3 + ; The final concentration of glycine is 0.1-0.5 mM; the neutral amino acid combination consists of serine with a final concentration of 0.1 mM, threonine with a final concentration of 0.2 mM, glycine with a final concentration of 0.2 mM, alanine with a final concentration of 0.3 mM and proline with a final concentration of 0.2 mM; the final concentration of GABA is 0.5 mM.

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