A bait for preventing and controlling red fire ants and a preparation method thereof

Abstract

The embodiment of the present invention discloses a bait for preventing and treating red fire ants and a preparation method thereof. The bait for preventing and treating red fire ants is composed of a termite killer and an attractant; the termite killer is composed of rotenone and neem oil; wherein the rotenone accounts for 0.01%-0.2% of the total mass of the bait, and the neem oil accounts for 0.01%-0.2% of the total mass of the bait; the remainder is an attractant. The bait for preventing and treating red fire ants in the embodiment of the present invention is composed of only a termite killer and an attractant formed by rotenone and neem oil, and the raw materials are less and the preparation is simple, which can save production and use costs, and the drug effect is slow (25-40 days to show the effect), and the worker ants can provide the bait to the mother ants, so that all the mother ants of red fire ants in the nest can be killed well, and the effect of completely killing ants is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field related to pest control, and in particular to a bait for controlling red imported fire ants and a preparation method thereof. Background Art

[0002] The Chinese patent application with publication number CN 111011367 A discloses a plant-based bait for killing red fire ants and its preparation method, which can attract red fire ants and have a good chronic poisoning effect. However, its raw materials are relatively large and complex. Summary of the invention

[0003] In view of this, one aspect of the present invention discloses a bait for controlling red fire ants.

[0004] The bait for preventing and controlling red fire ants consists of an anticide and an attractant; the anticide consists of rotenone and neem oil; wherein the rotenone accounts for 0.01%-0.2% of the total weight of the bait, the neem oil accounts for 0.01%-0.2% of the total weight of the bait; the remainder is the attractant.

[0005] According to a preferred embodiment of the present invention, the attractant is a beehive.

[0006] According to a preferred embodiment of the present invention, the attractant is beef powder.

[0007] According to a preferred embodiment of the present invention, the attractant is sucrose.

[0008] Another aspect of the present invention discloses a method for preparing bait for controlling red fire ants.

[0009] The preparation method of the bait for preventing and controlling red fire ants comprises the following steps:

[0010] adding rotenone to neem oil and mixing them evenly, so that the rotenone is dissolved in the neem oil to form a mixed solution;

[0011] adding the mixed liquid to the attractant and mixing them evenly to form a mixture;

[0012] Extruding and granulating the mixture to form bait particles with a particle size of 1.2-1.5 mm;

[0013] The bait particles are dried to obtain the bait for preventing and controlling red fire ants.

[0014] According to a preferred embodiment of the present invention, the attractant is a honeycomb; the honeycomb is broken into pieces; the mixed liquid is added to the broken honeycomb and mixed evenly to form the mixture, and the bait particles are dried by low-temperature drying.

[0015] According to a preferred embodiment of the present invention, the attractant is beef powder; the mixed liquid is added to the beef powder and mixed evenly to form the mixture, and the bait particles are dried at 50°C.

[0016] According to a preferred embodiment of the present invention, the attractant is sucrose; the sucrose is made into powder; the mixed liquid is added to the sucrose powder and mixed evenly to form the mixture, and the bait particles are dried by low-temperature drying.

[0017] The above one or more technical solutions in the bait for controlling red fire ants and the preparation method thereof provided by the embodiments of the present invention have at least one of the following technical effects:

[0018] 1. The bait for preventing and controlling red fire ants in the embodiment of the present invention is composed only of ant killer and attractant formed by rotenone and neem oil, with less raw materials and simple preparation, which can save production and use costs, and at the same time can have a better killing effect on red fire ants.

[0019] 2. The bait for preventing and controlling red fire ants in the embodiment of the present invention has a slow efficacy (it takes 25-40 days to show the effect), and the worker ants can provide the bait to the mother ants, thereby effectively killing all the mother ants in the nest, achieving the effect of completely killing the ants.

[0020] Some additional features of the present invention may be described in the following description. Some additional features of the present invention will be apparent to those skilled in the art through understanding of the following description or the production or operation of the embodiments. The features disclosed in the present invention may be realized and achieved through the practice or use of various methods, means and combinations of the specific embodiments described below. DETAILED DESCRIPTION

[0021] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention are clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0022] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0023] The embodiment of the invention discloses a bait for preventing and controlling red imported fire ants.

[0024] The bait for preventing and controlling red fire ants is composed of an anticide and an attractant. The anticide is composed of rotenone and neem oil. The rotenone accounts for 0.01%-0.2% of the total weight of the bait, the neem oil accounts for 0.01%-0.2% of the total weight of the bait, and the remainder is the attractant. The attractant can be honeycomb, beef powder or sucrose (such as white sugar or brown sugar).

[0025] The preparation method of the bait for preventing and controlling red fire ants comprises the following steps:

[0026] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0027] Add the mixture to the attractant and mix well to form a mixture.

[0028] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm.

[0029] The bait particles are dried to obtain the bait for preventing and controlling red fire ants.

[0030] When the attractant is a honeycomb, the honeycomb is broken, the mixed liquid is added to the broken honeycomb and mixed evenly to form a mixture, and the bait particles are dried by low-temperature drying.

[0031] When the attractant is beef powder, the mixed liquid is added into the beef powder and mixed evenly to form a mixture, and the bait particles are dried at 50°C.

[0032] When the attractant is sucrose, the sucrose is made into powder, the mixed liquid is added into the sucrose powder and mixed evenly to form a mixture, and the bait particles are dried by low-temperature drying.

[0033] The bait for preventing and controlling red fire ants in the embodiment of the present invention uses rotenone and neem oil as anticides. Both rotenone and neem oil have insecticidal effects, which can enhance the insecticidal effect. At the same time, rotenone can be added to neem oil to dissolve rotenone in the neem oil, thereby reducing the use of solvents.

[0034] The bait for preventing and controlling red fire ants in the embodiment of the present invention uses honeycomb, beef powder or sucrose (such as white sugar or brown sugar) as an attractant, which can achieve a good attracting effect. Especially the honeycomb, which mainly contains beeswax, resin, grease, pigment, tannin, sugar, organic acid, fatty acid, glycoside, enzyme and insect hormone, has a good attracting effect on red fire ants. At the same time, rotenone belongs to biological preparations and is easily oxidized and decomposed in the air. The components such as honeycomb beeswax, resin, grease can wrap rotenone and isolate oxygen, so that it is not easy to decompose, keep the efficacy lasting, and have good prevention effect.

[0035] The present invention is described in detail below through specific embodiments.

[0036] Example 1

[0037] The bait for preventing and controlling red fire ants in this embodiment consists of 0.01% of rotenone, 0.01% of neem oil and 99.98% of honeycomb.

[0038] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0039] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0040] Crush the honeycomb. Add the mixture to the crushed honeycomb and mix well to form a mixture.

[0041] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried by low-temperature drying to obtain bait for preventing and controlling red fire ants.

[0042] Example 2

[0043] The bait for preventing and controlling red fire ants in this embodiment consists of rotenone accounting for 0.05% of the total mass of the bait, neem oil accounting for 0.2% of the total mass of the bait, and honeycomb accounting for 99.75% of the total mass of the bait.

[0044] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0045] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0046] Crush the honeycomb. Add the mixture to the crushed honeycomb and mix well to form a mixture.

[0047] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried by low-temperature drying to obtain bait for preventing and controlling red fire ants.

[0048] Example 3

[0049] The bait for preventing and controlling red fire ants in this embodiment consists of rotenone accounting for 0.1% of the total mass of the bait, neem oil accounting for 0.2% of the total mass of the bait, and honeycomb accounting for 99.7% of the total mass of the bait.

[0050] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0051] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0052] Crush the honeycomb. Add the mixture to the crushed honeycomb and mix well to form a mixture.

[0053] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried by low-temperature drying to obtain bait for preventing and controlling red fire ants.

[0054] Example 4

[0055] The bait for preventing and controlling red fire ants in this embodiment consists of 0.01% of rotenone, 0.01% of neem oil and 99.98% of beef powder.

[0056] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0057] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0058] Add the mixture to the ground beef and mix well to form a mixture.

[0059] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried at a temperature of 50° C. to obtain bait for preventing and controlling red fire ants.

[0060] Example 5

[0061] The bait for preventing and controlling red fire ants in this embodiment consists of 0.05% of rotenone, 0.1% of neem oil and 99.85% of beef powder.

[0062] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0063] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0064] Add the mixture to the ground beef and mix well to form a mixture.

[0065] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried at a temperature of 50° C. to obtain bait for preventing and controlling red fire ants.

[0066] Example 6

[0067] The bait for preventing and controlling red fire ants in this embodiment consists of 0.1% of rotenone, 0.2% of neem oil and 99.7% of beef powder.

[0068] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0069] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0070] Add the mixture to the ground beef and mix well to form a mixture.

[0071] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried at a temperature of 50° C. to obtain bait for preventing and controlling red fire ants.

[0072] Example 7

[0073] The bait for preventing and controlling red fire ants in this embodiment consists of 0.05% of rotenone, 0.05% of neem oil and 99.9% of sucrose.

[0074] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0075] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0076] Powder the honey cane sugar. Add the mixture into the cane sugar powder and mix well to form a mixture.

[0077] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried by low-temperature drying to obtain bait for preventing and controlling red fire ants.

[0078] Example 8

[0079] The bait for preventing and controlling red fire ants in this embodiment consists of 0.1% of rotenone, 0.2% of neem oil and 99.7% of sucrose.

[0080] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0081] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0082] Powder the honey cane sugar. Add the mixture into the cane sugar powder and mix well to form a mixture.

[0083] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried by low-temperature drying to obtain bait for preventing and controlling red fire ants.

[0084] Example 9

[0085] The bait for preventing and controlling red fire ants in this embodiment consists of 0.2% of rotenone, 0.2% of neem oil and 99.6% of sucrose.

[0086] The preparation method of the bait for preventing and controlling red fire ants in this embodiment is as follows:

[0087] Add rotenone to neem oil and mix well to dissolve rotenone in neem oil to form a mixed solution.

[0088] Powder the honey cane sugar. Add the mixture into the cane sugar powder and mix well to form a mixture.

[0089] The mixture is extruded and granulated to form bait particles with a particle size of 1.2-1.5 mm. The bait particles are dried by low-temperature drying to obtain bait for preventing and controlling red fire ants.

[0090] The effect of the bait for controlling red fire ants according to the embodiment of the present invention will be described below using Example 2 as an example through experimental data.

[0091] 1 Test name

[0092] Field test on the efficacy of 0.05% rotenone bait in controlling red imported fire ants

[0093] 3 Test conditions

[0094] 3.1 Selection of test subjects, crops and varieties

[0095] Red Fire Ant (CSelenopsis invicta Buren)

[0096] Farmland ridges and abandoned land

[0097] 3.2 Environmental conditions

[0098] The experiment was conducted in Lishan Village, Dongge Town, Wenchang City, Hainan Province. The experimental site was abandoned land covered with weeds.

[0099] 4 Experimental design and arrangement

[0100] 4.1 Pharmacy

[0101] 4.1.1 Test Agents

[0102] 0.05% rotenone bait.

[0103] 4.1.2 Control Drugs

[0104] 0.045% indoxacarb (WP20160031), Guangdong Zhen Biotechnology Co., Ltd.

[0105] 4.1.3 Dosage and treatment number

[0106] Table 1 Experimental agents and experimental design

[0107]

[0108] 4.2 Test instruments / equipment

[0109] During the test weighing process, the electronic balance AUY200 No. 51S21 was used to directly weigh using conventional methods. For other information, please refer to "SOP-E0004 Use and Maintenance of Electronic Analytical Balance-V2.0".

[0110] 4.3 Community Arrangement

[0111] 4.3.1 Cell Arrangement

[0112] The plots were arranged in random blocks, and the experimental plots were arranged as follows:

[0113] I 1 2 5 4 3 II 2 1 3 5 4 III 3 4 2 1 5 IV 5 3 4 2 1

[0114] 4.3.2 Plot area and repetition

[0115] Plot area: Determined to be rectangular according to the conditions of the test site, each plot area is about 1200-1300m 2 , 8 to 11 ant nests per cell. A buffer zone greater than 3m to 5m is set between adjacent cells.

[0116] Number of repetitions: 4 times.

[0117] 4.4 Test methods

[0118] 4.4.1 Application method

[0119] Spread the agent in a circular pattern on the surface of the ant nest and within 50-100 cm of the vicinity, and evenly distribute it in the entire area. The blank control area is not treated.

[0120] 4.4.2 Application time and frequency

[0121] The pesticide was applied once on October 31, 2020, for a total of 1 application.

[0122] 4.4.3 Usage capacity

[0123] According to the experimental plan, each nest was given 15 / 20 / 25g of medicine respectively.

[0124] 4.4.4 Information on drugs for controlling other diseases and insect pests

[0125] During the experiment, no other pesticides were used to control other diseases and insect pests.

[0126] 5. Investigation, recording and measurement methods

[0127] 5.1 Meteorological and soil data

[0128] 5.1.1 Meteorological data

[0129] It was cloudy and rainless on the day of spraying, with a temperature of 23.10C.

[0130] 5.1.2 Soil data

[0131] The experimental site is abandoned farmland, overgrown with weeds and scattered small shrubs.

[0132] 5.2 Survey methods, time and frequency

[0133] 5.2.1 Survey time and frequency

[0134] Investigations were conducted before drug application (October 31), on the 7th day after drug application (November 7), on the 14th day after drug application (November 14), on the 21st day after drug application (November 21), on the 28th day after drug application (November 28), on the 35th day after drug application (December 5), on the 42nd day after drug application (December 12), on the 49th day after drug application (December 19), and the nests were dug up 49 days after drug application (December 19), for a total of 8 investigations.

[0135] 5.2.2 Survey Methods

[0136] Field survey method: Use the field survey method to investigate the number of live ant nests in the plot. Carefully check and mark all anthills in the plot before applying pesticides, insert a mark next to the live ant nests, and exclude dead nests. New live ant nests formed after applying pesticides are marked separately to distinguish them. After treatment, all dead red fire ants are found in the ant nests, and the live ant nests should also be marked.

[0137] Trapping method: Use the trapping method to monitor the number of worker ants. Use fresh ham sausage as bait. Cut the ham sausage into slices of 1 cm thick and 2 cm in diameter, put them in a monitoring bottle, place the bottle mouth close to the ground and fix it on the ground to trap, and mark it. Generally, the bait is placed at 5m to 10m or 1 piece / 100m. 2 Set baits at a certain density. Set more than 10 baits in each plot, and try to cover the entire plot. If the plot is too large or too small, the number of baits can be adjusted according to the size of the plot. Use baits during the period when red fire ants are more active. After a fixed time (usually 30 minutes to 60 minutes), cover the monitoring bottle, seal it, take it back, and mark it. Place the monitoring bottle in soapy water, open the bottle cap, and record the number of red fire ants in each bottle after the red fire ants crawl out, float, and die. If other types of ants are trapped, they should be properly stored and identified. In each subsequent survey, the monitoring bottle should be placed in the place where the first survey was conducted.

[0138] Digging nest method: Use the digging nest method to observe the survival of ant colonies. After 5-10 days of liquid nest filling or powder, granule and other treatments, randomly select 5 live ant nests marked before the test in each plot, disturb them first and then check whether there are red fire ants crawling out, then dig up the ant nests to observe whether there are surviving ants, and record the number of each insect stage. When digging nests, dig to the bottom of the ant nest, generally the soil depth is 50cm to 100cm. When conducting an investigation 5-7 weeks after the bait treatment, dig up all live ant nests marked before the test and produced during the test in each plot, check the survival of the ants, and record the number of each insect stage.

[0139] 5.2.3 Calculation method of drug efficacy

[0140] The control effect of live ant nests is calculated according to the following formula:

[0141]

[0142] The control effect of worker ants is calculated according to the following formula:

[0143]

[0144] Ant colony control effect: The survival of ants in ant nests is graded according to the ant colony classification standard.

[0145] All the nests were live ant nests, so it was determined that all ant colonies were graded as Level 3 before the treatment. Ant colony classification standard

[0146] as follows:

[0147] Level 0—No insect stage survives;

[0148] Level l: Only worker ants or snails and larvae survive;

[0149] Level 2: Only winged ants and other insect-like individuals except the queen ant survive;

[0150] Level 3: The queen ant and other insect-like individuals survive.

[0151] According to the survey results, the effect of ant colony control is calculated as follows:

[0152]

[0153] Comprehensive control effect: The weights of the control effect of ant nests, the control effect of worker ants, and the control effect of ant colonies are assigned to 0.3, 0.2, and 0.5, and the comprehensive control effect is calculated according to the following formula:

[0154] Comprehensive control effect = 0.3 × live ant nest control effect + 0.2 × worker ant control effect + 0.5 × ant colony control effect

[0155] 5.3 Direct impact on crops

[0156] The experiment was conducted on abandoned farmland and no pesticide damage was observed.

[0157] 6 Results

[0158] The protective effect of 0.05% rotenone bait on red fire ant workers is shown in Table 2-1.

[0159] Table 2-1 The efficacy of 0.05% rotenone bait against red imported fire ants

[0160]

[0161] The effectiveness of ant nest prevention is shown in Table 2-2.

[0162] Table 2-2 The efficacy of 0.05% rotenone bait against active nests of red imported fire ants

[0163]

[0164] Note: The data in the table are the average values ​​of 4 repetitions.

[0165] Ant colonies and comprehensive control effects are shown in Table 2-3.

[0166] Table 2-3 The control effect and comprehensive control effect of 0.05% rotenone bait on red imported fire ants

[0167] Processing number Ant colony level control effect (%) Significance of difference Comprehensive control effect 1 91.67 b 95.24 2 100.00 a 100.00 3 100.00 a 100.00 4 100.00 a 100.00

[0168] As can be seen from the above table, the present invention has a significant control effect on red fire ants. In addition, the bait for controlling red fire ants in the embodiment of the present invention has a slow effect (25-40 days to show the effect). After use, the number of red fire ants will become less and less, and all red fire ants in the nest can be killed well. However, general chemical agents have a rapid effect (3-5 days), can only kill worker ants, cause the mother ants to split the nest, and cannot completely kill the red fire ants.

[0169] In addition, the bait for preventing and controlling red fire ants in the embodiment of the present invention is composed of only ant killer and attractant formed by rotenone and neem oil, with less raw materials and simple preparation, which can save production and use costs.

[0170] It should be noted that all features disclosed in this specification, or steps in all methods or processes disclosed, except for mutually exclusive features and / or steps, can be combined in any manner.

[0171] In addition, the above specific embodiments are exemplary, and those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also belong to the disclosure scope of the present invention and fall within the protection scope of the present invention. Those skilled in the art should understand that the description of the present invention is illustrative and does not constitute a limitation of the claims. The protection scope of the present invention is defined by the claims and their equivalents.

Claims

1. A bait for preventing and controlling red fire ants, characterized in that: The bait for preventing and controlling red fire ants is composed of a termite killer and an attractant; The ant killer consists of rotenone and neem oil, wherein the rotenone accounts for 0.05% of the total mass of the bait, and the neem oil accounts for 0.2% of the total mass of the bait; and the attractant is a honeycomb accounting for 99.75% of the total mass of the bait.

2. A method for preparing a bait for controlling red imported fire ants according to claim 1, characterized in that: The preparation method of the bait for preventing and controlling red fire ants comprises the following steps: adding rotenone to neem oil and mixing them evenly, so that the rotenone is dissolved in the neem oil to form a mixed solution; adding the mixed liquid to the attractant and mixing them evenly to form a mixture; Extruding and granulating the mixture to form bait particles with a particle size of 1.2-1.5 mm; Drying the bait particles to obtain the bait for preventing and controlling red fire ants; The attractant is a beehive; The honeycomb is broken; the mixed liquid is added into the broken honeycomb and mixed evenly to form the mixture, and the bait particles are dried by low-temperature drying.

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