Method, device, system and medium for processing red blood cell concentration changes

Abstract

The present invention relates to the technical field of red blood cell detection, and in particular to a method, device, system and medium for processing changes in red blood cell concentration; the application discloses a method for processing changes in red blood cell concentration, comprising the following steps: receiving an extraction instruction, executing an extraction action of a centrifugal cup through a peristaltic pump; acquiring collection values ​​in real time through an infrared cell sensor, and performing instantaneous drop jump identification on all acquired collection values ​​to obtain an identification result; when the identification result is an instantaneous drop jump, comparing the first jump collection value after the instantaneous drop jump with a first threshold; when the first jump collection value is less than or equal to the first threshold, determining that the red blood cells are being extracted; the processing method described in the application can automatically identify whether the red blood cells in the centrifugal cup have been emptied, without the need for manual visual inspection, thereby greatly saving cell separation time and labor costs.

Description

Technical Field

[0001] The present invention relates to the technical field of red blood cell detection, and in particular to a method, device, system and medium for processing red blood cell concentration changes. Background Art

[0002] Cell therapy has become an emerging treatment method, and cell separation methods play an important role in cell therapy. Cell separation methods often use centrifuge cups to separate mononuclear cells. During the cell separation process, a blood sample needs to be injected into the centrifuge cup to separate the red blood cell layer, and the red blood cell layer needs to be evacuated from the centrifuge cup. Currently, identifying whether the red blood cell layer has been evacuated requires manual visual inspection, and the operator must be present at all times, which has the disadvantage of low efficiency. In addition, when the operator leaves, the evacuation time point may be missed, which will delay the next operation and cause a waste of time. Summary of the Invention

[0003] In order to overcome the shortcomings of the existing technology, the present invention provides a method, device, system and medium for processing red blood cell concentration changes. The infrared cell sensor collects the difference in the AD value change of the centrifuge cup outlet pipeline, automatically identifies whether the red blood cells in the centrifuge cup have been emptied, and then proceeds to the next step of operation without manual visual inspection, greatly saving cell separation time and labor costs.

[0004] The technical solution adopted by the present invention to solve its technical problem is:

[0005] A method for processing changes in red blood cell concentration comprises the following steps:

[0006] Receive the extraction instruction and execute the extraction action of the centrifugal cup through the peristaltic pump;

[0007] The infrared cell sensor acquires the collected values ​​in real time, and performs instantaneous drop jump recognition on all the collected values ​​to obtain the recognition results;

[0008] When the recognition result is an instantaneous falling jump, comparing the first jump acquisition value after the instantaneous falling jump with a first threshold;

[0009] When the first jump acquisition value is less than or equal to the first threshold, determining that the state is red blood cell extraction;

[0010] After determining that the red blood cell extraction state is reached, calculating in real time a gradual change difference between the collected value and the first jump collected value, and determining whether the gradual change difference is greater than or equal to a second threshold;

[0011] When the gradient difference is greater than or equal to the second threshold, it is determined that the red blood cells are exhausted and the extraction action is stopped.

[0012] Furthermore, the instantaneous drop jump identification is performed on all acquired collected values, and after obtaining the identification result, the method includes:

[0013] When the recognition result is a non-instantaneous falling jump, the collected values ​​continue to be acquired in real time.

[0014] Furthermore, after comparing the first transition acquisition value after the instantaneous drop transition with the first threshold, the method further includes:

[0015] When the first jump acquisition value is greater than the first threshold, it is determined that interference noise is extracted, and the acquisition value continues to be acquired in real time.

[0016] Furthermore, after determining whether the gradient difference is greater than or equal to the second threshold, the method further includes:

[0017] When the gradual difference is less than the second threshold, the gradual difference between the collected value and the first jump collected value continues to be calculated in real time.

[0018] Furthermore, the instantaneous drop jump identification is performed on all acquired collected values ​​to obtain the identification result, including:

[0019] Extracting the collected values ​​within a preset time period from the current moment from all collected values, and determining the extracted collected values ​​as the values ​​to be identified;

[0020] Identify the number of falling edges of all the values ​​to be identified to obtain a number identification result;

[0021] When the number recognition result is characterized as one, it is determined that the recognition result is an instantaneous falling jump.

[0022] Furthermore, the step of identifying the number of falling edges of all the values ​​to be identified to obtain a number identification result includes:

[0023] Calculating the difference between two adjacent values ​​to be identified to obtain a plurality of jump differences;

[0024] Determining whether each of the jump differences is greater than or equal to a preset jump threshold;

[0025] Count the number of the jump difference values ​​that are greater than or equal to the preset jump threshold, and record the number as the number identification result.

[0026] Furthermore, the real-time acquisition of collected values ​​by the infrared cell sensor includes:

[0027] When the volume of the cell suspension in the centrifugal cup is reduced to a preset value, the collection value is obtained in real time.

[0028] A device for processing red blood cell concentration changes, comprising:

[0029] The receiving module is used to receive the extraction instruction and execute the extraction action of the centrifugal cup through the peristaltic pump;

[0030] The recognition module is used to obtain the collected values ​​in real time through the infrared cell sensor, and perform instantaneous drop jump recognition on all the collected values ​​to obtain the recognition results;

[0031] a comparing module, configured to compare a first jump acquisition value after the instantaneous falling jump with a first threshold value when the recognition result is an instantaneous falling jump;

[0032] a determination module, configured to determine that the red blood cell extraction state is in progress when the first jump acquisition value is less than or equal to the first threshold;

[0033] a judgment module, configured to calculate, in real time, a gradual difference between the collected value and the first jump collected value after determining that the red blood cell extraction state is reached, and to judge whether the gradual difference is greater than or equal to a second threshold;

[0034] The stopping module is configured to determine that the red blood cells are exhausted and stop the extraction action when the gradient difference is greater than or equal to the second threshold.

[0035] A red blood cell concentration change processing system comprises a controller for executing the red blood cell concentration change processing method, a centrifugal cup and a peristaltic pump.

[0036] A computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the above-mentioned method for processing red blood cell concentration changes is implemented.

[0037] The beneficial effects of the present invention are:

[0038] (1) The present invention obtains the collected values ​​in real time through the infrared cell sensor, and identifies the instantaneous drop jump of all the collected values. When the first jump collected value is less than or equal to the first threshold value, it is determined to be the red blood cell extraction state; when the gradual difference is greater than or equal to the second threshold value, it is determined to be the red blood cell emptying state. Therefore, during the process from red blood cell extraction to emptying, the red blood cell sensor can be used for real-time monitoring and identification, and it can automatically identify whether the red blood cells in the centrifuge cup are empty, without the need for manual visual inspection, which greatly saves cell separation time and labor costs.

[0039] (2) The present invention identifies instantaneous falling jumps on all acquired acquisition values. When the first jump acquisition value is greater than a first threshold, it is determined to be the extraction of interference noise. The acquisition values ​​are continued to be acquired in real time until the first jump acquisition value is less than or equal to the first threshold, and the state of red blood cell extraction is determined. Therefore, before determining the state of red blood cell extraction, interference signals caused by subtle changes in the outside world or pipelines during the extraction process are eliminated, thereby improving the accuracy of extraction.

[0040] (3) The present invention can save the life and power consumption of the infrared cell sensor by obtaining the collection value in real time when the volume of the cell suspension in the centrifuge cup is reduced to a preset value after determining that the red blood cell extraction state has been reached. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] The present invention will be further described below with reference to the accompanying drawings and examples.

[0042] Figure 1 This is a workflow diagram of a method for processing changes in red blood cell concentration according to the present invention;

[0043] Figure 2 It is a specific schematic diagram of the peristaltic pump performing the extraction action of the centrifugal cup in the method for processing the change of red blood cell concentration described in the present invention;

[0044] Figure 3 This is a principle block diagram of a device for processing red blood cell concentration changes according to the present invention;

[0045] Explanation of the accompanying symbols: 1. centrifuge cup; 2. peristaltic pump; 3. infrared cell sensor; 4. control valve; 5. packaging bag; 10. receiving module; 20. identification module; 30. comparison module; 40. determination module; 50. judgment module; 60. stop module. DETAILED DESCRIPTION

[0046] In order to facilitate understanding by those skilled in the art, the present invention will be further described below with reference to the embodiments. The contents mentioned in the embodiments are not intended to limit the present invention.

[0047] like Figure 1 A method for processing changes in red blood cell concentration is shown, comprising the following steps:

[0048] Receive the extraction instruction and execute the extraction action of the centrifugal cup through the peristaltic pump.

[0049] It can be understood that the extraction instruction is an instruction to start the peristaltic pump to extract red blood cells. The extraction instruction can be obtained by clicking on the human-computer interaction interface, or by automatically triggering when the action of extracting red blood cells is involved in the operation of the process step. The peristaltic pump is used for the directional flow of liquid in a non-contact peristaltic pipeline. The centrifuge cup is a container for high-speed centrifugal rotation of the blood sample, which can allow the blood sample to be stratified in the container, that is, blood centrifugation can divide the blood into three relatively obvious layers, including a plasma layer, a white blood cell and platelet layer, and a red blood cell layer. The extraction action is the action of extracting the red blood cell layer after the centrifuge cup is subjected to high-speed centrifugal rotation and stratification from the outlet flow channel in the centrifuge cup (the outlet flow channel is close to the wall of the centrifuge cup because the red blood cell layer will fall on the outermost layer and close to the wall of the centrifuge cup during centrifugation). After the red blood cell layer is extracted, the white blood cell and platelet layers will be extracted.

[0050] The collected values ​​are acquired in real time by the infrared cell sensor, and instantaneous drop jump recognition is performed on all the acquired collected values ​​to obtain the recognition results.

[0051] It can be understood that the infrared cell sensor is a non-contact sensor that detects the concentration of red blood cells in the pipe and converts the detected red blood cell concentration into a digital signal value (AD value). The digital signal can reflect the red blood cell concentration. Preferably, the infrared cell sensor 3 is a transmission type photoelectric infrared sensor that can be used to detect red blood cell concentration less than or equal to 2×10 9 / mL of red blood cells, the collected value is the AD value collected and converted by the infrared cell sensor into the red blood cell concentration, the process of identifying the instantaneous decrease jump is to collect the AD value within a preset time period from the current time point through the infrared cell sensor, continuously update the AD value within the preset time period from the current time point as time goes by, subtract each two adjacent AD values ​​from each other for all updated AD values, and when the absolute value of the subtraction difference is greater than the preset value, and the subsequent subtraction differences at the time point are similar and within the preset tolerance range, the identification result is determined to be the identification process of an instantaneous decrease jump at the time point, the process of identifying the instantaneous decrease jump can also be to extract the instantaneous decrease features of all collected AD values ​​through a trained neural network model, classify the extracted instantaneous decrease features, classify whether there is an instantaneous decrease jump, and thus identify the identification result of the instantaneous decrease jump, the instantaneous decrease feature is a trend feature of a sudden decrease in adjacent AD values, and the identification result reflects the result of whether there is an instantaneous decrease jump.

[0052] When the identification result is an instantaneous falling jump, the first jump acquisition value after the instantaneous falling jump is compared with the first threshold.

[0053] It is understandable that when the recognition result is that there is a transient drop jump, the first AD value collected after the transient drop jump is recorded as the first jump acquisition value, and the first jump acquisition value is compared with the first threshold value. The first threshold value is the AD value corresponding to the preset red blood cell concentration, which can be 2×10 9 / mL, and compare the first jump acquisition value with the first threshold value.

[0054] When the first jump acquisition value is less than or equal to the first threshold, it is determined to be in the red blood cell extraction state.

[0055] It is understandable that when the empty tube is extracted, there is no red blood cell liquid in the tube, and the AD value collected is the value when the tube is empty. The AD value is close to the value when it is empty, and the AD value is larger. When the red blood cells are extracted and pass through the infrared cell sensor, the AD value with red blood cell concentration is immediately collected. At this time, the infrared light will be absorbed by the red blood cell liquid in the tube, and its AD value becomes smaller. When the first jump collection value is less than or equal to the first threshold value, it is determined to be the red blood cell extraction state, which indicates that there is red blood cell liquid flowing through the infrared cell sensor.

[0056] After determining that the red blood cell extraction state is reached, a gradual difference between the collected value and the first jump collected value is calculated in real time to determine whether the gradual difference is greater than or equal to a second threshold.

[0057] It can be understood that after determining that the red blood cell extraction state is in progress, the AD value of the infrared cell sensor is collected in real time, and the difference between the collected value and the first jump collected value is calculated in real time, and the difference is recorded as the gradual difference. The second threshold is a preset value, which is the difference between the AD value of the red blood cells initially extracted based on historical statistics and the AD value when white blood cells or mononuclear cells are started to be extracted, or the difference between the AD value when the red blood cell concentration is very low.

[0058] When the gradient difference is greater than or equal to the second threshold, it is determined that the red blood cells are exhausted and the extraction action is stopped.

[0059] Specifically, the peristaltic pump performs the extraction action of the centrifugal cup, such as Figure 2As shown, the specific extraction process and corresponding settings are as follows: an infrared cell sensor 3 is installed on the liquid outlet pipeline of the centrifuge cup 1, and the red blood cell layer is extracted from the centrifuge cup 1 through the peristaltic pump 2. During the extraction process, the infrared cell sensor 3 is used to collect the red blood cell concentration in the pipeline in real time, and a control valve 4 is set (a control valve is set at the input end of the sub-packaging bag and the input end of the centrifuge cup) to control the red blood cell layer to flow into the sub-packaging bag 5. More specifically, the infrared cell sensor 3 is a transmission type photoelectric infrared sensor that can be used to detect when the red blood cell concentration is less than or equal to 2×10 9 / mL of red blood cells.

[0060] It is understandable that during the process of extracting red blood cells, the concentration of red blood cells in the pipeline will change from high to low, and the color will change from dark to light. The collected value obtained in real time by the infrared cell sensor will show a gradual attenuation process; the collected value is specifically the AD value.

[0061] Specifically, when the red blood cell layer begins to be extracted from the centrifuge cup, the infrared cell sensor is used to collect the AD value changes in the pipeline in real time. When the red blood cells in the red blood cell layer flow through the infrared cell sensor through the pipeline, the collected AD value will drop instantaneously when red blood cells flow from no to some. The collected values ​​are obtained in real time by the infrared cell sensor, and all the collected values ​​are identified as transient decrease jumps to obtain an identification result. When the identification result is a transient decrease jump, the first jump collected value after the transient decrease jump is compared with the first threshold value.

[0062] When the first jump acquisition value is less than or equal to the first threshold, it is determined to be in the red blood cell extraction state.

[0063] After determining that the red blood cell extraction state is in progress, the gradual difference between the collection value and the first jump collection value is calculated in real time to determine whether the gradual difference is greater than or equal to the second threshold value; in the process of extracting the red blood cells, the red blood cell concentration in the pipeline will change from high to low (the concentration is so low that there are almost no red blood cells), and the color will change from dark to light (almost no red color presented by red blood cells, almost colorless), and the AD value collected by the infrared cell sensor will show a gradual attenuation process.

[0064] When the gradient difference is greater than or equal to the second threshold, it is determined that the red blood cells are exhausted and the extraction action is stopped;

[0065] In one embodiment, the instantaneous drop jump identification of all acquired collected values ​​and obtaining the identification result includes:

[0066] When the recognition result is a non-instantaneous falling jump, the collected values ​​continue to be acquired in real time.

[0067] It is understandable that the recognition result includes instantaneous falling jump and non-instantaneous falling jump. The non-instantaneous falling jump indicates that there is no instantaneous falling process in the collected value. If the recognition result is non-instantaneous falling jump, the collected value continues to be acquired in real time.

[0068] In one embodiment, the instantaneous drop jump identification is performed on all acquired collected values ​​to obtain the identification result, including:

[0069] The collected values ​​within a preset time period from the current moment are extracted from all collected values, and the extracted collected values ​​are determined as the values ​​to be identified.

[0070] The number of falling edges of all the values ​​to be identified is identified to obtain a number identification result.

[0071] It can be understood that the process of identifying the number of falling edges is to calculate the difference between the two adjacent values ​​to be identified, determine whether the difference is greater than or equal to the preset jump threshold, and summarize the number that is greater than or equal to the preset jump threshold. The number identification result reflects the result of the number of falling edges.

[0072] When the number recognition result is characterized as one, it is determined that the recognition result is an instantaneous falling jump.

[0073] It can be understood that when the number recognition result is not one, it means that the red blood cell extraction operation is not being performed, so the recognition result is determined to be a non-instantaneous falling jump. When the number recognition result is one, the recognition result is determined to be an instantaneous falling jump.

[0074] In one embodiment, the step of identifying the number of falling edges of all the values ​​to be identified to obtain a number identification result includes:

[0075] The differences between any two adjacent values ​​to be identified are calculated to obtain a plurality of jump differences.

[0076] Determine whether each of the jump differences is greater than or equal to a preset jump threshold.

[0077] It is understandable that the preset jump threshold can be set according to needs and determined according to the accuracy of the infrared cell sensor.

[0078] Count the number of the jump difference values ​​that are greater than or equal to the preset jump threshold, and record the number as the number identification result.

[0079] It can be understood that the number identification result reflects the number of the jump difference values ​​that are greater than or equal to the preset jump threshold.

[0080] The present invention achieves the following steps: obtaining a plurality of jump difference values ​​by calculating the difference between two adjacent values ​​to be identified; judging whether each jump difference value is greater than or equal to a preset jump threshold value; counting the number of jump difference values ​​that are greater than or equal to the preset jump threshold value, and recording the number as the number identification result. In this way, it is possible to automatically judge whether it is a jump number based on the difference value without manual judgment, and output the number identification result, thereby improving the efficiency of identification.

[0081] In one embodiment, when the recognition result is an instantaneous falling jump, comparing the first jump acquisition value after the instantaneous falling jump with a first threshold value includes:

[0082] When the first jump acquisition value is greater than the first threshold, it is determined that interference noise is extracted, and the acquisition value is continued in real time;

[0083] It can be understood that when the ΔD value calculation is performed and the difference ΔD between the AD values ​​corresponding to the current time point n and the previous acquisition time point n-1 is less than or equal to the threshold, the identification result is an instantaneous downward jump; when the first jump acquisition value is greater than the first threshold, it is determined to be extracted interference noise, and the acquisition value continues to be acquired in real time. The extracted interference noise is an interference signal caused by subtle changes in the outside world or pipeline during the extraction process.

[0084] In one embodiment, when the first jump acquisition value is less than or equal to the first threshold, it is determined to be in the red blood cell extraction state.

[0085] After determining that the red blood cell extraction state is reached, a gradual difference between the collected value and the first jump collected value is calculated in real time to determine whether the gradual difference is greater than or equal to a second threshold.

[0086] When the gradual difference is less than the second threshold, the gradual difference between the collected value and the first jump collected value continues to be calculated in real time.

[0087] It is understandable that when the gradient difference is greater than or equal to the second threshold, it is determined that the red blood cells are completely evacuated and the extraction operation is stopped. Specifically, the second threshold can be set to a range of 4 / 5 to 9 / 10 of the first threshold.

[0088] In one embodiment, the real-time acquisition of collected values ​​by an infrared cell sensor includes:

[0089] After the red blood cell extraction state is determined, when the volume of the cell suspension in the centrifugal cup is reduced to a preset value, the collection value is obtained in real time.

[0090] It can be understood that the preset value can be set to the volume of the cell suspension in the centrifuge cup that is about to be drained, for example, the preset value is 1 / 3 of the volume of the cell suspension; after determining that the red blood cells are being extracted, when the volume of the cell suspension measured by the weighing sensor is reduced to the preset value, the AD value does not need to be collected in real time, which can save the life and power consumption of the infrared cell sensor.

[0091] In one embodiment, a device for processing changes in red blood cell concentration is provided, which corresponds one-to-one to the method for processing changes in red blood cell concentration in the above embodiment, such as Figure 3 As shown, the red blood cell concentration change processing device includes:

[0092] The receiving module 10 is used to receive an extraction instruction and execute an extraction action of the centrifugal cup through the peristaltic pump;

[0093] The recognition module 20 is used to obtain the collected values ​​in real time through the infrared cell sensor, and perform instantaneous drop jump recognition on all the collected values ​​to obtain the recognition results;

[0094] A comparison module 30 is configured to compare the first jump acquisition value after the instantaneous falling jump with a first threshold value when the recognition result is an instantaneous falling jump;

[0095] a determination module 40, configured to determine that the red blood cell extraction state is in progress when the first jump acquisition value is less than or equal to the first threshold;

[0096] A judgment module 50 is configured to calculate, in real time, a gradual difference between the collected value and the first jump collected value after determining that the red blood cell extraction state is reached, and to determine whether the gradual difference is greater than or equal to a second threshold;

[0097] The stopping module 60 is configured to determine that the red blood cells are exhausted and stop the extraction action when the gradient difference is greater than or equal to the second threshold.

[0098] The specific definition of the red blood cell concentration change processing device can be found in the definition of the red blood cell concentration change processing method above, and will not be repeated here. Each module in the above-mentioned red blood cell concentration change processing device can be implemented in whole or in part by software, hardware, or a combination thereof. Each of the above-mentioned modules can be embedded in or independent of the processor in the computer device in the form of hardware, or can be stored in the memory of the computer device in the form of software, so that the processor can call and execute the operations corresponding to each of the above modules.

[0099] In one embodiment, a system for processing red blood cell concentration changes includes a controller for executing the above-mentioned method for processing red blood cell concentration changes, a centrifugal cup, and a peristaltic pump.

[0100] Each module in the controller described above may be implemented in whole or in part through software, hardware, or a combination thereof. Each module may be embedded in or independent of a processor in a computer device in the form of hardware, or may be stored in a memory in the computer device in the form of software, so that the processor can call and execute the corresponding operations of each module.

[0101] In one embodiment, a computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the above-mentioned method for processing red blood cell concentration changes is implemented.

[0102] Those skilled in the art will understand that all or part of the processes in the above-mentioned embodiment methods can be implemented by instructing the relevant hardware through a computer program, and the computer program can be stored in a non-volatile computer-readable storage medium. When the computer program is executed, it can include the processes of the embodiments of the above-mentioned methods. Among them, any reference to memory, storage, database or other media used in the embodiments provided in this application may include non-volatile and / or volatile memory. Non-volatile memory may include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM) or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in many forms such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), Synchronous Link DRAM (SLDRAM), Rambus direct RAM (RDRAM), direct RAM bus dynamic RAM (DRDRAM), and RAM bus dynamic RAM (RDRAM), among others.

[0103] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the scope of protection of the present invention.

Claims

1. A method for processing changes in red blood cell concentration, characterized in that: The following steps are involved: Receiving the extraction instruction, executing the extraction action of the centrifuge cup through the peristaltic pump; the peristaltic pump extracts the red blood cell layer from the centrifuge cup; An infrared cell sensor is installed on the liquid outlet pipe of the centrifuge cup, and the collected values ​​are obtained in real time through the infrared cell sensor. All the collected values ​​are subjected to instantaneous drop jump recognition to obtain recognition results; When the recognition result is an instantaneous falling jump, comparing the first jump acquisition value after the instantaneous falling jump with a first threshold; When the first jump acquisition value is less than or equal to the first threshold, determining that the state is red blood cell extraction; After determining that the red blood cell extraction state is reached, calculating in real time a gradual change difference between the collected value and the first jump collected value, and determining whether the gradual change difference is greater than or equal to a second threshold; When the gradient difference is greater than or equal to the second threshold, it is determined that the red blood cells are exhausted and the extraction action is stopped; The instantaneous drop jump identification is performed on all acquired collected values ​​to obtain the identification result, including: Extracting the collected values ​​within a preset time period from the current moment from all collected values, and determining the extracted collected values ​​as the values ​​to be identified; Identify the number of falling edges of all the values ​​to be identified to obtain a number identification result; When the number recognition result is characterized as one, it is determined that the recognition result is an instantaneous falling jump.

2. The method for processing red blood cell concentration changes according to claim 1, characterized in that: After performing instantaneous drop jump identification on all acquired collected values ​​and obtaining the identification result, the method includes: When the recognition result is a non-instantaneous falling jump, the collected values ​​continue to be acquired in real time.

3. The method for processing red blood cell concentration changes according to claim 1, characterized in that: After comparing the first transition acquisition value after the instantaneous drop transition with the first threshold, the method further comprises: When the first jump acquisition value is greater than the first threshold, it is determined that interference noise is extracted, and the acquisition value continues to be acquired in real time.

4. The method for processing red blood cell concentration changes according to claim 1, wherein: After determining whether the gradient difference is greater than or equal to the second threshold, the method further includes: When the gradual difference is less than the second threshold, the gradual difference between the collected value and the first jump collected value continues to be calculated in real time.

5. The method for processing red blood cell concentration changes according to claim 1, wherein: The step of identifying the number of falling edges of all the values ​​to be identified to obtain a number identification result includes: Calculating the difference between two adjacent values ​​to be identified to obtain a plurality of jump differences; Determining whether each of the jump differences is greater than or equal to a preset jump threshold; Count the number of the jump difference values ​​that are greater than or equal to the preset jump threshold, and record the number as the number identification result.

6. A device for processing changes in red blood cell concentration, characterized in that: include: The receiving module is used to receive the extraction instruction and execute the extraction action of the centrifugal cup through the peristaltic pump; A peristaltic pump draws the red blood cell layer from the centrifuge cup; The identification module is used to install an infrared cell sensor on the liquid outlet pipe of the centrifuge cup, obtain the collected values ​​in real time through the infrared cell sensor, and perform instantaneous drop jump identification on all the collected values ​​to obtain the identification results; The instantaneous drop jump identification is performed on all acquired collected values ​​to obtain the identification result, including: Extracting the collected values ​​within a preset time period from the current moment from all collected values, and determining the extracted collected values ​​as the values ​​to be identified; Identify the number of falling edges of all the values ​​to be identified to obtain a number identification result; When the number recognition result is characterized as one, determining that the recognition result is an instantaneous falling jump; a comparing module, configured to compare a first jump acquisition value after the instantaneous falling jump with a first threshold value when the recognition result is an instantaneous falling jump; a determination module, configured to determine that the red blood cell extraction state is in progress when the first jump acquisition value is less than or equal to the first threshold; a judgment module, configured to calculate, in real time, a gradual difference between the collected value and the first jump collected value after determining that the red blood cell extraction state is reached, and to judge whether the gradual difference is greater than or equal to a second threshold; The stopping module is configured to determine that the red blood cells are exhausted and stop the extraction action when the gradient difference is greater than or equal to the second threshold.

7. A red blood cell concentration change processing system, characterized in that: The invention comprises a controller for executing the method for processing changes in red blood cell concentration according to any one of claims 1 to 5, a centrifugal cup and a peristaltic pump.

8. A computer-readable storage medium storing a computer program, characterized in that: When the computer program is executed by a processor, the method for processing red blood cell concentration changes according to any one of claims 1 to 5 is implemented.

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