Germinated brown rice and preparation method thereof

By using a mixed enhancement solution of zinc sulfate and sodium selenite in the preparation process of germinated brown rice for circulating humidification, and combining magnetic field-induced germination and microwave drying technology, the problems of low germination efficiency and insufficient zinc-selenium content are solved, and efficient and economical preparation of zinc-rich selenium-rich germinated brown rice is achieved.

CN115918851BActive Publication Date: 2025-04-25HEFEI UNIV OF TECH
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Patent Information

Application Number
CN202310031291.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-01-09
Publication Date
2025-04-25
Estimated Expiration
2043-01-09

AI Technical Summary

Technical Problem

The existing germinated brown rice has low germination efficiency and insufficient zinc and selenium content, which cannot meet the normal needs of the human body, and there are problems of high cost and low efficiency in industrial production.

Method used

After the sodium hypochlorite disinfection treatment was used, the mixed enhancement solution of zinc sulfate and sodium selenite was circulated and humidified. Combined with magnetic field-induced germination and microwave drying technology, germinated brown rice rich in zinc, selenium and GABA was prepared.

Benefits of technology

It significantly improves the zinc and selenium content and germination efficiency of germinated brown rice, enhances the nutritional value and health care functions of brown rice, and reduces production costs and drying time.

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Abstract

The invention discloses a germinated brown rice and a preparation method thereof. The preparation method comprises: using sodium hypochlorite to disinfect brown rice to obtain disinfected brown rice; using a strengthening solution to perform a cyclic humidification treatment on the disinfected brown rice to obtain a cyclic humidification treated brown rice; wherein the strengthening solution comprises zinc sulfate and sodium selenite; and, the cyclic humidification treated brown rice is subjected to magnetic field induced germination and microwave drying treatment to obtain germinated brown rice. The preparation method provided by the present invention can significantly increase the content of γ-aminobutyric acid (GABA), a beneficial active ingredient in germinated brown rice, so that the prepared brown rice is rich in micronutrients zinc and selenium and rich in GABA; at the same time, the prepared germinated brown rice has a health care effect of preventing cardiovascular diseases.
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Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and particularly relates to germinated brown rice and a preparation method thereof. Background Art

[0002] Rice is the main dietary source of trace nutrients such as zinc and selenium in the human body. Brown rice refers to whole-grain rice that still contains the germ, endosperm and cortex after the husk is removed. Most of the dietary fiber, biotin, vitamins, minerals and other nutrients in rice are stored in the bran layer and germ. Therefore, brown rice retains all the nutrients of rice better than polished rice. However, the phytate, bran wax and cellulose contained in the bran layer of brown rice are the main reasons for its rough taste and poor cooking properties.

[0003] Zinc is one of the essential trace elements for the human body, and its content ranks second only to iron. Zinc can accelerate children's growth and development, promote wound healing, and maintain the normal functioning of immune cells and other immune organs. About one-third of the world's population is zinc deficient, which can cause symptoms such as hair loss, decreased intelligence, loss of appetite, and decreased immunity. In developing countries, zinc deficiency has become the fifth leading cause of disease and death. Zinc in food alone cannot meet the normal needs of the human body. In countries where rice is the staple food, staple food fortification has become an ideal way to supplement zinc.

[0004] Selenium is an essential trace element for the human body. It has the functions of protecting the structure and function of cell membranes, scavenging free radicals, improving human immunity and preventing the production of toxins in the body. Many countries and regions in the world are in a state of selenium deficiency or potential selenium deficiency. Selenium deficiency in the body can lead to pancreatic islet damage caused by abnormal B cell structure and function, a decrease in the number of phagocytes and lymphocytes, and suppression or decline of immune ability. Dietary selenium is the main way for the human body to absorb it, but the selenium content in food under natural conditions is very low.

[0005] After germination, the nutritional value and edible quality of brown rice are improved. Brown rice germination can activate and release a large number of enzymes (protease, amylase, cellulase, etc.) contained in brown rice, catalyze a series of biochemical reactions, and thus change the composition of brown rice. In particular, the content of the bioactive ingredient γ-aminobutyric acid (GABA) is significantly increased after germination. GABA is a natural amino acid composed of non-proteins, which participates in the inhibitory nerve conduction of the central nervous system and has multiple functions such as calming excitement, lowering blood pressure, relieving arteriosclerosis, and improving liver function. In addition, during the germination process, brown rice can effectively absorb zinc and selenium in the soaking solution, and brown rice itself has biological enrichment and conversion effects. Despite this, the industrial production of germinated brown rice still has some disadvantages, such as high cost and low germination efficiency.

[0006] In the prior art, patent CN112715852A discloses a process for preparing germinated brown rice, which comprises the following steps: S1: weighing an appropriate amount of brown rice as raw material, screening it, screening out the damaged brown rice, and then picking out the moldy and deteriorated brown rice, so that high-quality brown rice can be left; S2: weighing an appropriate amount of citric acid solution, and then putting the high-quality brown rice in S1 into the citric acid solution and soaking it for a period of time; S3: draining the soaked brown rice in S2 and rinsing it with clean water. The germinated brown rice prepared by this method can improve the nutritional content of the germinated brown rice, but the zinc and selenium content in the brown rice is not improved. Summary of the invention

[0007] The main purpose of the present invention is to provide a germinated brown rice and a preparation method thereof, so as to overcome the deficiencies of the prior art.

[0008] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention includes:

[0009] An embodiment of the present invention provides a method for preparing germinated brown rice, comprising:

[0010] The brown rice is disinfected with sodium hypochlorite to obtain the disinfected brown rice;

[0011] The sterilized brown rice is subjected to a cyclic humidification treatment using a strengthening solution to obtain cyclic humidification treated brown rice; wherein the strengthening solution comprises zinc sulfate and sodium selenite;

[0012] And, the brown rice treated with the circulating humidification is subjected to magnetic field induced germination and microwave drying treatment to obtain germinated brown rice.

[0013] The embodiment of the present invention also provides germinated brown rice prepared by the aforementioned preparation method.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The present invention selects zinc sulfate solution and sodium selenite solution as strengthening solution materials. The selected strengthening solution (zinc sulfate and sodium selenite solution) is low in price and easy to obtain, suitable for large-scale industrial production, and the zinc sulfate solution has high solubility at neutral pH, which is conducive to the better absorption of zinc solution in the brown rice germination process;

[0016] (2) The present invention uses a mixed strengthening solution to carry out a cyclic humidification treatment on brown rice, and utilizes the bioaccumulation effect of brown rice itself to effectively absorb zinc sulfate and sodium selenite in the strengthening solution. Due to the biological transformation effect of the brown rice itself during the germination process, the inorganic zinc-selenium solution is converted into a biologically available form, so that the germinated brown rice not only has rich nutritional value, but also is rich in micronutrients such as zinc and selenium, and can be used as a dietary choice for supplementing zinc and selenium. At the same time, the cyclic humidification method can avoid the loss of water-soluble GABA when producing germinated brown rice by the traditional immersion method;

[0017] (3) After the brown rice is subjected to cyclic humidification and strengthening treatment, the present invention exposes the brown rice to a magnetic field, utilizes the stress effect of the magnetic field on germination, regulates the physiological changes in the germination process, thereby promoting the germination of the brown rice, improving the germination efficiency, and helping to save the cost of the germinated brown rice production process. In addition, the combined treatment of cyclic humidification and magnetic field not only realizes the uniform and effective zinc and selenium enrichment of the germinated brown rice during the cyclic humidification process, but also promotes germination through magnetic field stress, thereby significantly improving the nutrition and active ingredient content of the brown rice, especially the improvement of the GABA content;

[0018] (4) The present invention adopts microwave drying technology to replace traditional hot air drying technology, which avoids the problem that traditional hot air has high temperature and serious damage to the internal structure of the material and low efficiency, can significantly shorten the drying time of brown rice, improve drying efficiency, and has the advantages of environmental protection, controllability, energy saving and high efficiency. DETAILED DESCRIPTION

[0019] In order to solve the problems of poor edible quality and low nutritional value caused by the rough taste, low germination efficiency and low zinc and selenium content of brown rice, which cannot meet the normal needs of the human body, the present invention proposes a method for preparing germinated brown rice, which prepares germinated brown rice rich in zinc, selenium and GABA by spraying a mixed strengthening solution of zinc sulfate and sodium selenite during a circulating humidification process and utilizing the biological enrichment and conversion of germinated brown rice and the stress of a magnetic field.

[0020] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Specifically, as one aspect of the technical solution of the present invention, a method for preparing germinated brown rice includes:

[0022] The brown rice is disinfected with sodium hypochlorite to obtain the disinfected brown rice;

[0023] The sterilized brown rice is subjected to a cyclic humidification treatment using a strengthening solution to obtain cyclic humidification treated brown rice; wherein the strengthening solution comprises zinc sulfate and sodium selenite;

[0024] And, the brown rice treated with the circulating humidification is subjected to magnetic field induced germination and microwave drying treatment to obtain germinated brown rice.

[0025] In some preferred embodiments, the preparation method specifically comprises: soaking the brown rice in a sodium hypochlorite solution for disinfection, and then removing impurities and floating particles to obtain the disinfected brown rice.

[0026] Furthermore, the concentration of the sodium hypochlorite solution is 0.5-1.5wt%.

[0027] Furthermore, the soaking time is 5 to 15 minutes.

[0028] In some preferred embodiments, the preparation method specifically comprises: spreading the sterilized brown rice on a perforated germination plate in a constant temperature and humidity culture device, and then performing a cyclic humidification treatment on the brown rice using a strengthening solution.

[0029] Furthermore, the cyclic humidification treatment includes: spraying the enhanced solution on the sterilized brown rice for 3 minutes every 20 minutes, and performing the cyclic treatment 3 times.

[0030] Furthermore, the temperature of the constant temperature and humidity culture device is 25-30° C., and the relative humidity is 90%.

[0031] In some preferred embodiments, the concentration of zinc sulfate in the fortified solution is 20-30 mg / L, and the concentration of sodium selenite is 10-30 mg / L.

[0032] In some preferred embodiments, the preparation method specifically comprises: exposing the brown rice treated with circulating humidification to a magnetic field environment for induction treatment, and then placing it in a constant temperature and humidity incubator for germination treatment.

[0033] Furthermore, the magnetic field intensity of the magnetic field environment is 10 mT, and the induction treatment time is 50-70 min;

[0034] And / or, the germination treatment is performed at a temperature of 32 to 36° C. for 22 to 30 hours.

[0035] In some preferred embodiments, the microwave drying power used in the microwave drying process is 400 W, and the drying time is 20 to 30 minutes.

[0036] In some more specific embodiments, the method for preparing germinated brown rice comprises:

[0037] (1) Disinfection of brown rice: Weigh an appropriate amount of brown rice, soak it in a 0.5%-1.5% sodium hypochlorite solution for disinfection, soak for 5-15 min, wash it three times until the solution is neutral and remove impurities and floating particles;

[0038] (2) Circular humidification and strengthening treatment: In a constant temperature and humidity incubator, the incubator temperature is adjusted to 25-30° C. and the relative humidity is maintained at 90%. The sterilized brown rice is spread on a perforated germination tray in the constant temperature and humidity incubator. Zinc sulfate and sodium selenite are configured into a mixed strengthening solution, and the brown rice is circulated and sprayed with the mixed strengthening solution; wherein the concentration of the zinc sulfate solution is 20-30 mg / L, and the concentration of the sodium selenite solution is 10-30 mg / L; the brown rice is sprayed with the mixed strengthening solution for 3 min every 20 min, and the excess mixed strengthening solution flows out from the aperture of the germination tray. The strengthening solution at the bottom of the germination tray is replaced every 4 hours, and the circulated treatment is repeated 3 times;

[0039] (3) Magnetic field induced germination treatment: The brown rice after cyclic humidification and strengthening treatment was washed three times, and then the brown rice was exposed to a magnetic field strength of 10 mT to induce germination, and the magnetic field treatment was performed for 50-70 min; then the brown rice was placed in a constant temperature and humidity incubator at a germination temperature of 32-36° C. for 22-30 h;

[0040] (4) Microwave drying treatment: The germinated brown rice is placed in a microwave drying oven and dried at a drying power of 400 W for 20-30 minutes to obtain zinc-selenium-rich germinated brown rice.

[0041] In the present invention, the magnetic field is a safe, mild, naturally occurring component of the environment. As a non-thermal processing technology, the magnetic field can be used to regulate and control the germination process of grains, effectively forcing a series of physiological changes in grain seeds, thereby improving the nutritional value, flavor quality and germination efficiency of grains. Compared with other drying methods, the internal and external temperature gradients and pressure gradients of microwave drying are consistent with the migration direction of water in the material. The drying efficiency is high and there is basically no damage to the internal structure of the food. It is also widely used in food drying due to its environmental protection, controllable, energy-saving and high efficiency. The research significance is great.

[0042] Brown rice retains the fiber part of the outer layer, resulting in a rough taste of the rice grains and poor edible quality. In addition, the trace nutrients (zinc and selenium) contained in brown rice itself are insufficient and cannot meet people's requirements for dietary zinc and selenium supplementation. The germinated brown rice prepared by the existing germination technology has a low germination efficiency and is not conducive to large-scale industrial production. Based on the above existing problems, the present invention utilizes the physiological change process of germination, adopts a mixed solution of zinc sulfate and sodium selenite to carry out cyclic humidification treatment on brown rice to achieve zinc and selenium strengthening of germinated brown rice, exposes the strengthened brown rice to a magnetic field for a period of time, utilizes the stress effect of the magnetic field on the brown rice, regulates and controls the germination process of the brown rice, and never improves the germination efficiency of the brown rice. At the same time, under the action of the magnetic field, a series of enzyme activities of the brown rice in the germination process are further improved, the nutritional value of the germinated brown rice is significantly improved, and the content of the important bioactive component (GABA) in the brown rice is further increased. Subsequently, the germinated brown rice is subjected to microwave drying treatment, which greatly shortens the drying time, thereby efficiently preparing a germinated brown rice rich in zinc, selenium and GABA at the same time. The prepared germinated brown rice has high nutritional value and certain health care functions, is a suitable zinc and selenium dietary supplement option, and helps to prevent the occurrence of cardiovascular diseases.

[0043] Another aspect of the embodiments of the present invention further provides germinated brown rice prepared by the aforementioned preparation method.

[0044] The zinc-selenium-rich germinated brown rice prepared by the present invention can effectively alleviate the zinc and selenium deficiency problems worldwide. In the process of inducing brown rice germination by magnetic field, the content of the beneficial active ingredient gamma-aminobutyric acid (GABA) is also increased, so that the prepared brown rice is rich in micronutrients zinc and selenium and GABA under the combined treatment of cyclic humidification and magnetic field stress. The drying rate of the germinated brown rice is effectively improved by using microwave drying technology. At the same time, the prepared germinated brown rice has the health care effect of preventing cardiovascular diseases.

[0045] The technical solution of the present invention is further described in detail below in conjunction with several preferred embodiments. This embodiment is implemented on the premise of the technical solution of the invention, and a detailed implementation method and specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.

[0046] Unless otherwise specified, the experimental materials used in the following examples can be purchased from conventional biochemical reagent companies.

[0047] Example 1

[0048] (1) Disinfection of brown rice

[0049] Weigh an appropriate amount of brown rice, soak it in 0.5% sodium hypochlorite solution for disinfection, soak for 5 minutes, wash three times until the solution is neutral and remove impurities and floating particles.

[0050] (2) Circulating humidification and enhanced treatment

[0051] In a constant temperature and humidity incubator, the incubator temperature is adjusted to 25°C and the relative humidity is maintained at 90%. The sterilized brown rice is spread on a perforated germination tray in the constant temperature and humidity incubator. Zinc sulfate and sodium selenite are configured into a mixed strengthening solution, and the brown rice is sprayed with the mixed strengthening solution in a cycle. The concentration of the zinc sulfate solution is 20 mg / L, and the concentration of the sodium selenite solution is 10 mg / L. The mixed strengthening solution is sprayed on the brown rice for 3 minutes every 20 minutes, and the excess mixed strengthening solution flows out from the aperture of the germination tray. The strengthening solution at the bottom of the germination tray is replaced every 4 hours, and the cycle treatment is repeated 3 times.

[0052] (3) Magnetic field induced germination treatment

[0053] The soaked brown rice was washed three times, and then exposed to a magnetic field of 10 mT to induce germination for 50 min. The brown rice was then placed in a constant temperature and humidity incubator at a germination temperature of 32° C. for 22 h.

[0054] (4) Microwave drying

[0055] The germinated brown rice was placed in a microwave drying oven and dried for 20 minutes at a drying power of 400 W to obtain zinc-selenium-rich germinated brown rice.

[0056] Example 2

[0057] (1) Disinfection of brown rice

[0058] Weigh an appropriate amount of brown rice, soak it in 0.8% sodium hypochlorite solution for disinfection, soak for 8 minutes, wash three times until the solution is neutral and remove impurities and floating particles.

[0059] (2) Circulating humidification and enhanced treatment

[0060] In a constant temperature and humidity incubator, the incubator temperature is adjusted to 27°C and the relative humidity is maintained at 90%. The sterilized brown rice is spread on a perforated germination tray in the constant temperature and humidity incubator. Zinc sulfate and sodium selenite are configured into a mixed strengthening solution, and the brown rice is sprayed with the mixed strengthening solution in a cycle. The concentration of the zinc sulfate solution is 23 mg / L, and the concentration of the sodium selenite solution is 15 mg / L. The mixed strengthening solution is sprayed on the brown rice for 3 minutes every 20 minutes, and the excess mixed strengthening solution flows out from the aperture of the germination tray. The strengthening solution at the bottom of the germination tray is replaced every 4 hours, and the cycle treatment is repeated 3 times.

[0061] (3) Magnetic field induced germination treatment

[0062] The soaked brown rice was washed three times, and then exposed to a magnetic field of 10 mT to induce germination for 55 min. The brown rice was then placed in a constant temperature and humidity incubator at a germination temperature of 33° C. for 24 h.

[0063] (4) Microwave drying

[0064] The germinated brown rice was placed in a microwave drying oven and dried for 23 minutes at a drying power of 400 W to obtain zinc-selenium-rich germinated brown rice.

[0065] Example 3

[0066] (1) Disinfection of brown rice

[0067] Weigh an appropriate amount of brown rice, soak it in 1.0% sodium hypochlorite solution for disinfection, soak for 10 minutes, wash three times until the solution is neutral and remove impurities and floating particles.

[0068] (2) Circulating humidification and enhanced treatment

[0069] In a constant temperature and humidity incubator, the incubator temperature is adjusted to 28°C and the relative humidity is maintained at 90%. The sterilized brown rice is spread on a perforated germination tray in the constant temperature and humidity incubator. Zinc sulfate and sodium selenite are configured into a mixed strengthening solution, and the brown rice is sprayed with the mixed strengthening solution in a cycle. The concentration of the zinc sulfate solution is 25 mg / L, and the concentration of the sodium selenite solution is 20 mg / L. The mixed strengthening solution is sprayed on the brown rice for 3 minutes every 20 minutes, and the excess mixed strengthening solution flows out from the aperture of the germination tray. The strengthening solution at the bottom of the germination tray is replaced every 4 hours, and the cycle treatment is repeated 3 times.

[0070] (3) Magnetic field induced germination treatment

[0071] The soaked brown rice was washed three times, and then exposed to a magnetic field of 10 mT to induce germination for 60 min. The brown rice was then placed in a constant temperature and humidity incubator at a germination temperature of 34°C for 26 h.

[0072] (4) Microwave drying

[0073] The germinated brown rice was placed in a microwave drying oven and dried for 25 minutes at a drying power of 400 W to obtain zinc-selenium-rich germinated brown rice.

[0074] Example 4

[0075] (1) Disinfection of brown rice

[0076] Weigh an appropriate amount of brown rice, soak it in 1.3% sodium hypochlorite solution for disinfection, soak it for 13 minutes, wash it three times until the solution is neutral and remove impurities and floating particles.

[0077] (2) Circulating humidification and enhanced treatment

[0078] In a constant temperature and humidity incubator, the incubator temperature is adjusted to 29°C and the relative humidity is maintained at 90%. The sterilized brown rice is spread on a perforated germination tray in the constant temperature and humidity incubator. Zinc sulfate and sodium selenite are configured into a mixed strengthening solution, and the brown rice is sprayed with the mixed strengthening solution in a cycle. The concentration of the zinc sulfate solution is 28 mg / L, and the concentration of the sodium selenite solution is 25 mg / L. The mixed strengthening solution is sprayed on the brown rice for 3 minutes every 20 minutes, and the excess mixed strengthening solution flows out from the aperture of the germination tray. The strengthening solution at the bottom of the germination tray is replaced every 4 hours, and the cycle treatment is repeated 3 times.

[0079] (3) Magnetic field induced germination treatment

[0080] The soaked brown rice was washed three times, and then exposed to a magnetic field of 10 mT to induce germination for 65 min. The brown rice was then placed in a constant temperature and humidity incubator at a germination temperature of 35° C. for 28 h.

[0081] (4) Microwave drying

[0082] The germinated brown rice was placed in a microwave drying oven and dried for 28 minutes at a drying power of 400 W to obtain zinc-selenium-rich germinated brown rice.

[0083] Example 5

[0084] (1) Disinfection of brown rice

[0085] Weigh an appropriate amount of brown rice, soak it in 1.5% sodium hypochlorite solution for disinfection, soak it for 15 minutes, wash it three times until the solution is neutral and remove impurities and floating particles.

[0086] (2) Circulating humidification and enhanced treatment

[0087] In a constant temperature and humidity incubator, the incubator temperature is adjusted to 30°C and the relative humidity is maintained at 90%. The sterilized brown rice is spread on a perforated germination tray in the constant temperature and humidity incubator. Zinc sulfate and sodium selenite are configured into a mixed strengthening solution, and the brown rice is sprayed with the mixed strengthening solution in a cycle. The concentration of the zinc sulfate solution is 30 mg / L, and the concentration of the sodium selenite solution is 30 mg / L. The mixed strengthening solution is sprayed on the brown rice for 3 minutes every 20 minutes, and the excess mixed strengthening solution flows out from the aperture of the germination tray. The strengthening solution at the bottom of the germination tray is replaced every 4 hours, and the cycle treatment is repeated 3 times.

[0088] (3) Magnetic field induced germination treatment

[0089] The soaked brown rice was washed three times, and then exposed to a magnetic field of 10 mT to induce germination for 70 min. The brown rice was then placed in a constant temperature and humidity incubator at a germination temperature of 36° C. for 30 h.

[0090] (4) Microwave drying

[0091] The germinated brown rice was placed in a microwave drying oven and dried for 30 minutes at a drying power of 400 W to obtain zinc-selenium-rich germinated brown rice.

[0092] Comparative Example 1

[0093] The preparation method of this comparative example is basically the same as that of Example 3, except that deionized water is used instead of the mixed enhanced solution of zinc sulfate and sodium selenite in step (2) for the circulating humidification treatment.

[0094] Comparative Example 2

[0095] The preparation method of this comparative example is basically the same as that of Example 3, except that in step (2), the mixed strengthening solution of zinc sulfate and sodium selenite is subjected to ordinary soaking treatment.

[0096] Comparative Example 3

[0097] The preparation method of this comparative example is basically the same as that of Example 3, except that the magnetic field stress-induced germination treatment in step (3) is not performed.

[0098] In order to further illustrate the technical effect of the present invention, relevant samples were obtained from Examples 1-5 and Comparative Examples 1-3 for measurement.

[0099] 1. Determination of germination rate of prepared germinated brown rice

[0100] After the brown rice germinated, 100 grains of brown rice were randomly selected to calculate the germination rate. The calculation formula of the germination rate (%) is as follows.

[0101] Germination rate (%) = (number of germinated brown rice / total number of tested brown rice) × 100

[0102] 2. Determination of zinc and selenium content in prepared germinated brown rice

[0103] Concentrated nitric acid was added to a polytetrafluoroethylene digestion tube containing 0.5 g of rice flour for pre-digestion. Next, the digestion solution was digested at 200°C until the digestion solution was completely clear and transparent. The digestion solution was cooled to room temperature and the zinc and selenium contents in the sample were determined using an inductively coupled plasma mass spectrometer (ICP-MS).

[0104] 3. Determination of GABA content in prepared germinated brown rice

[0105] Brown rice flour and ethanol were mixed in a ratio of 1:5 and shaken in a water bath at 60°C for 2 hours. After centrifugation, the supernatant was added with boric acid buffer, phenol and sodium hypochlorite solution in sequence. After mixing evenly, the reaction solution was soaked in boiling water for 10 minutes and ice bathed for 15 minutes until it turned blue-green. Then 2 mL of ethanol was added and the sample absorbance was measured at 630 nm.

[0106] Table 1 shows the germination rate of the prepared germinated brown rice.

[0107] Group Germination rate (%) Example 1 90.33±0.77 Example 2 92.46±0.54 Example 3 97.67±0.94 Example 4 93.59±0.69 Example 5 94.50±0.73 Comparative Example 1 78.04±1.02 Comparative Example 2 75.43±0.81 Comparative Example 3 72.43±0.75

[0108] Table 2 shows the zinc content and selenium content of the prepared germinated brown rice

[0109] Group Zinc content (mg / kg) Selenium content (mg / kg) Example 1 46.38±0.16 0.59±0.07 Example 2 48.15±0.12 0.62±0.05 Example 3 50.26±0.15 0.63±0.10 Example 4 49.10±0.54 0.61±0.09 Example 5 47.94±0.27 0.62±0.08 Comparative Example 1 26.70±0.25 0.08±0.03 Comparative Example 2 42.07±0.31 0.43±0.08 Comparative Example 3 35.30±0.26 0.31±0.04

[0110] Table 3 shows the GABA content of the prepared germinated brown rice

[0111] Group GABA content (mg / 100g) Example 1 12.83±0.09 Example 2 13.10±0.12 Example 3 13.66±0.18 Example 4 13.52±0.10 Example 5 13.09±0.08 Comparative Example 1 10.79±0.26 Comparative Example 2 7.17±0.15 Comparative Example 3 8.56±0.12

[0112] As shown in Table 1, compared with Comparative Examples 1-3, the germination rate of the germinated brown rice prepared in Example 3 is the highest; as shown in Table 2, compared with Comparative Examples 1-3, the zinc and selenium contents in the germinated brown rice prepared in Example 3 are the highest; as shown in Table 3, compared with Comparative Examples 1-3, the GABA content in the germinated brown rice prepared in Example 3 is higher. It can be seen that the present invention effectively improves the zinc and selenium content of brown rice by using a mixed solution of zinc sulfate and sodium selenite to strengthen brown rice during the cyclic humidification process, further improves the germination rate of brown rice by using a magnetic field to force brown rice to germinate, and effectively shortens the drying time by using microwave drying technology, which helps to improve the industrial production efficiency of germinated brown rice and reduce costs. On this basis, the content of GABA, a representative bioactive ingredient in brown rice, is significantly improved, and the cyclic humidification treatment avoids the loss of GABA during the traditional soaking treatment process. The nutritional value of the germinated brown rice is further improved. The germinated brown rice prepared by the present invention has high nutritional value, high germination efficiency, is rich in trace nutrients zinc and selenium, and has good health care functions and economic benefits.

[0113] In addition, the inventors of this case also referred to the aforementioned embodiments and conducted experiments with other raw materials, process operations, and process conditions described in this specification, and obtained relatively ideal results.

[0114] It should be understood that the technical solution of the present invention is not limited to the above-mentioned specific implementation cases. Any technical deformation made according to the technical solution of the present invention without departing from the scope of protection of the purpose of the present invention and the claims shall fall within the protection scope of the present invention.

Claims

1. A method for preparing germinated brown rice, characterized in that include: The brown rice is disinfected with sodium hypochlorite to obtain the disinfected brown rice; The sterilized brown rice is subjected to a cyclic humidification treatment using a strengthening solution to obtain cyclic humidification treated brown rice; The strengthening solution comprises zinc sulfate and sodium selenite; wherein the cyclic humidification treatment comprises: spraying the strengthening solution on the sterilized brown rice for 3 minutes every 20 minutes, and cyclically treating the sterilized brown rice 3 times; the concentration of zinc sulfate in the strengthening solution is 20-30 mg / L, and the concentration of sodium selenite is 10-30 mg / L; And, exposing the brown rice treated with circulating humidification to a magnetic field environment for induction treatment, and then placing it in a constant temperature and humidity incubator for germination treatment and microwave drying treatment to obtain germinated brown rice; wherein the magnetic field intensity of the magnetic field environment is 10mT, the induction treatment time is 50-70min; the germination treatment temperature is 32-36°C, and the time is 22-30h.

2. The preparation method according to claim 1, characterized in that Specifically include: The brown rice is soaked in a sodium hypochlorite solution for disinfection, and then impurities and floating particles are removed to obtain the disinfected brown rice.

3. The preparation method according to claim 2, characterized in that: The concentration of the sodium hypochlorite solution is 0.5-1.5wt%; and / or the immersion time is 5-15min.

4. The preparation method according to claim 1, characterized in that Specifically include: The sterilized brown rice is spread on a perforated germination plate in a constant temperature and humidity culture device, and then the brown rice is subjected to a cyclic humidification treatment using a strengthening solution.

5. The preparation method according to claim 4, characterized in that: The temperature of the constant temperature and humidity culture device is 25-30° C., and the relative humidity is 90%.

6. The preparation method according to claim 1, characterized in that: The microwave drying power used in the microwave drying process is 400W, and the drying time is 20-30min.

Citation Information

Patent Citations

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