Method for determining spectral parameters suitable for regulating sugar metabolism

Abstract

The application discloses a spectrum parameter determination method suitable for regulating sugar metabolism, and comprises the following steps: exposing each of a plurality of to-be-tested objects to a plurality of light stimulation modes respectively; for each of the light stimulation modes, performing a blood sugar tolerance test on the plurality of to-be-tested objects respectively after a preset exposure time, so as to obtain first blood sugar tolerance information of the plurality of to-be-tested objects; determining second blood sugar tolerance information corresponding to the plurality of light stimulation modes respectively according to the first blood sugar tolerance information of the plurality of to-be-tested objects; and determining a spectrum parameter corresponding to a light stimulation mode with the optimal blood sugar tolerance improvement capability as a target spectrum parameter according to the second blood sugar tolerance information of the plurality of light stimulation modes. The application is based on the spectrum sensitivity of self-photoreceptor ganglion cells, and the target spectrum parameter capable of improving sugar metabolism is obtained by using the blood sugar tolerance test, so that an implementable light-providing scheme can be provided for effectively improving sugar metabolism.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of spectroscopy, and in particular to a method for determining a spectral parameter suitable for regulating sugar metabolism. BACKGROUND

[0002] Glucose is the transport form of sugar in blood, and plays a major role in sugar metabolism in the body. Blood glucose metabolism refers to the delivery of glucose in blood to various tissues and cells in the body through blood circulation, and the consumption of the glucose through oxidation to provide energy for the body, or the storage of the glucose by converting it into other nutrients. In a normal body, there is fine regulation of blood glucose homeostasis, so as to maintain the blood glucose at a relatively constant level. Through a glucose tolerance test, we can understand the body's ability to regulate blood glucose concentration. The level of blood glucose concentration regulation ability directly reflects the health level of the body. Long-term exposure to an unfavorable blood glucose metabolism environment increases the risk of diseases, such as diabetes, obesity, emotional disorders, and cancer.

[0003] The regulation of sugar metabolism in the body is an extremely complex process, and the study of its mechanism involves multiple key sites in the central and peripheral nervous systems and the participation of hormones. A large number of studies have shown that multiple nuclei of the hypothalamus control the energy metabolism of the body: for example, the preoptic area, the lateral hypothalamic area, the dorsal medial hypothalamic nucleus, the ventromedial hypothalamic nucleus, the arcuate nucleus, and the paraventricular nucleus of the hypothalamus. In addition, the absence of melanocortin receptor-4 in neurons in the lateral hypothalamic area can impair glucose tolerance in mice. Relative to the central nervous system, peripheral tissues receive signals from the central nervous system to regulate blood glucose homeostasis, including brown adipose tissue, muscle tissue, liver tissue, and islet tissue. Similarly, hormones also play a key role in maintaining blood glucose homeostasis. For example, insulin signals can suppress glucose production in the liver and lower blood glucose, and insulin can also activate the agouti-related protein neurons in the central arcuate nucleus to suppress glucose production in the liver.

[0004] Although there is a large amount of basic research on the regulation of sugar metabolism, the current method for improving sugar metabolism that can be implemented is through long-term adjustment of dietary patterns and increase of physical activity exercise. Light is an important external factor that regulates various physiological functions of the body, such as mood, cognition, and rhythm. In addition, studies have shown that changes in central rhythm can lead to disorders in sugar and lipid metabolism and other physiological indicators. A large number of epidemiological studies have found that excessive light exposure increases the risk of diabetes and obesity. Therefore, exploring how light regulates sugar metabolism will provide a theoretical basis for the treatment of metabolism-related diseases, and develop effective and implementable solutions. SUMMARY

[0005] In view of the above problems, the main purpose of the present application is to provide a spectrum parameter determination method suitable for regulating sugar metabolism, based on the spectral sensitivity of the photosensitive ganglion cells, the blood glucose tolerance of the test object under different light stimulation modes in a short period is detected by blood glucose tolerance test, and then the spectrum parameter of the light stimulation mode capable of improving sugar metabolism is obtained, so as to provide an implementable light scheme for effectively improving sugar metabolism, and provide a theoretical basis and implementable strategy for treating sugar metabolism disorders.

[0006] In order to achieve the above-mentioned purpose, the technical scheme of the present application is as follows:

[0007] A spectrum parameter determination method suitable for regulating sugar metabolism, comprising the following steps: exposing each of a plurality of test objects to a plurality of light stimulation modes, wherein the plurality of light stimulation modes include blue light, red light and dark light or include white light and dark light; for each of the light stimulation modes, after a preset exposure time, the blood glucose tolerance test is performed on the plurality of test objects respectively, to obtain the first blood glucose tolerance information of the plurality of test objects, wherein the first blood glucose tolerance information is used to characterize the blood glucose tolerance of the test object; according to the first blood glucose tolerance information of the plurality of test objects, the second blood glucose tolerance information corresponding to the plurality of light stimulation modes is determined, wherein the second blood glucose tolerance information is used to characterize the blood glucose tolerance improvement ability of the light stimulation mode; according to the second blood glucose tolerance information of the plurality of light stimulation modes, the spectrum parameter corresponding to the light stimulation mode with the optimal blood glucose tolerance improvement ability is determined as the target spectrum parameter.

[0008] Based on the above technical scheme, the present application has at least one or part of the following beneficial effects:

[0009] The spectrum parameter determination method provided by the present application determines the target spectrum parameter capable of effectively increasing the utilization ability of the body to glucose in the blood by testing the regulation degree of different spectrum components on the blood glucose tolerance.

[0010] Based on the determined target spectrum parameter, a light spectrum strategy capable of improving the blood glucose metabolism ability of experimental animals and humans can be provided to help improve the metabolic health of experimental animals and humans. For example, when the body is challenged by energy, the spectrum parameter in the environment can be adjusted to improve the ability of the body to metabolize sugar

[0011] Unlike the traditional methods for improving the ability of sugar metabolism, such as changing the diet mode (time-restricted diet, intermittent diet) and increasing physical exercise, the target spectrum parameter determined based on the present application can provide a new strategy method for improving the ability of sugar metabolism. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1is a flow chart of the method for determining the spectrum parameters suitable for regulating sugar metabolism in the embodiments of the present application;

[0013] Figure 2 is a flow chart of the method for determining the spectrum parameters suitable for regulating sugar metabolism in the embodiments of the present application;

[0014] Figure 3 is a spectrum parameter diagram of multiple light stimulation modes in the embodiments of the present application;

[0015] Figure 4 is a result diagram of improving the blood sugar tolerance of mice and humans under white light stimulation and dark stimulation modes in the embodiments of the present application;

[0016] Figure 5 is a result diagram of improving the blood sugar tolerance of mice under different light stimulation modes in the embodiments of the present application;

[0017] Figure 6 is a result diagram of improving the blood sugar tolerance of humans under different light stimulation modes in the embodiments of the present application. DETAILED DESCRIPTION

[0018] In order to make the objectives, technical solutions and advantages of the present application clearer, further detailed description will be made to the present application in combination with specific embodiments.

[0019] Through literature research, it is found that the body's perception of light is mainly through three kinds of photoreceptor cells in the retina, rod cells, cone cells and ganglion cells (ipRGC) capable of self-photoreception through melanopsin. The rod and cone cells of the retina are mainly responsible for image recognition-imaging vision, and the self-photoreceptor ganglion cells show strong sensitivity to blue light and are mainly responsible for non-imaging visual functions such as rhythm, pupillary light reflex, body temperature and mood regulation. In animal studies, long-term changes in light patterns can disturb the central rhythm through self-photoreceptor ganglion cells, thereby affecting blood sugar metabolism. Due to the complexity of life and work in industrialized society, light is needed for illumination in various scenes, and excessive light exposure is inevitable for humans. Therefore, by studying the effects of different spectral components on sugar metabolism, different spectral components can be given to assist human work and life at specific times and environments, thereby improving sugar metabolism. Finally, the regulation of different spectral components on blood sugar metabolism will provide effective and implementable solutions for improving human health.

[0020] In the implementation of the present application, it is found that the blood glucose tolerance can be regulated by light stimulation in a short period of time, and then the blood glucose tolerance of the test object under different light stimulation modes in a short period of time is detected by using the blood glucose tolerance test, and the target spectrum parameter of the light stimulation mode capable of improving sugar metabolism is obtained, thereby providing an effective and implementable light strategy method for improving sugar metabolism, and providing theoretical basis and strategy support for treating sugar metabolism disorders and other diseases.

[0021] Specifically, according to the embodiment of the present application, a spectrum parameter determination method suitable for regulating sugar metabolism is provided. Figure 1 is a flow chart of the spectrum parameter determination method suitable for regulating sugar metabolism in the embodiment of the present application; Figure 2 is a flow chart of the spectrum parameter determination method suitable for regulating sugar metabolism for different test objects in the embodiment of the present application. As shown in Figure 1 and Figure 2 The spectrum parameter determination method includes the following operations S110-S140.

[0022] In operation S110, each test object in a plurality of test objects is exposed to a plurality of light stimulation modes, wherein the plurality of light stimulation modes represent a plurality of light environments with different spectrum parameters, including blue light, red light and dark light stimulation modes, or including white light and dark light stimulation modes;

[0023] In operation S120, for each light stimulation mode, a blood glucose tolerance test is performed on the plurality of test objects after a preset exposure time to obtain first blood glucose tolerance information of the plurality of test objects, wherein the first blood glucose tolerance information is used to represent the blood glucose tolerance of the test object;

[0024] In operation S130, according to the first blood glucose tolerance information of the plurality of test objects, second blood glucose tolerance information corresponding to the plurality of light stimulation modes is determined, wherein the second blood glucose tolerance information is used to represent the blood glucose tolerance improvement ability of the light stimulation mode;

[0025] In operation S140, according to the second blood glucose tolerance information of the plurality of light stimulation modes, the spectrum parameter corresponding to the light stimulation mode with the optimal blood glucose tolerance improvement ability is determined as the target spectrum parameter.

[0026] According to the embodiment of the present application, in operation S110, as shown in Figure 2 The test object can be experimental animal mice or recruited healthy personnel, etc. It is found by experiment that mice and human body have similar spectrum parameter test results, indicating that the target spectrum parameter determined by the present application has certain reliability and universality when applied to regulate blood glucose metabolism.

[0027] According to an embodiment of the present application, in operation S110, the plurality of light stimulation modes include stimulation modes of blue light, red light and dark light or stimulation modes of white light and dark light, and the spectral parameters include spectral wavelength ranges and spectral intensities. It can be understood that, since the same object under test is involved in different light stimulation modes, the regulation of sugar metabolism between different light stimulation modes can be made comparable. In some embodiments, the regulation of blood glucose metabolism under different spectral intensities of light environment in the same spectral wavelength range can be studied, but the regulation of blood glucose metabolism under different spectral wavelength ranges of light environment is more concerned in the present application.

[0028] For example, Figure 3 is a schematic diagram of spectral parameters of the plurality of light stimulation modes in an embodiment of the present application; as Figure 3 shown, the blue light stimulation mode, the red light stimulation mode and the white light stimulation mode are provided by corresponding LEDs as light sources. In the A diagram, the spectral wavelength range of the blue light is 450-480 nm, in the B diagram, the spectral wavelength range of the red light is 610-630 nm, and in the C diagram, the white light is formed by mixing blue light, yellow light and red light with a spectral wavelength range of 450-480 nm. In the D diagram, the total number of photons per unit time per unit area of the three kinds of spectra is similar, i.e., the spectral intensities are basically the same. The dark light stimulation mode (hereinafter referred to as dark stimulation mode) corresponds to a dark light environment without light source.

[0029] According to an embodiment of the present application, in operation S110, the selection of the plurality of light stimulation modes is made in consideration of whether the plurality of light stimulation modes to which the object under test is exposed have significant sensitivity differences for the photosensitive ganglion cells, and in order to determine the influence of different spectral ranges on blood glucose metabolism and to avoid exposing the same object under test to the white light stimulation mode and the blue / red light stimulation mode at the same time. According to an embodiment of the present application, in operation S120, the preset exposure time is 2-3 hours, i.e., after the object under test such as a mouse or a test person is exposed to the light stimulation mode for 2-3 hours, a blood glucose tolerance test is performed. In order to test the regulation ability of the light stimulation mode on the blood glucose metabolism of the object under test, operation S120 specifically includes sub-operation S121-sub-operation S122:

[0030] In sub-operation S121, after detecting the blood glucose value of the object under test under the basic condition, the object under test is given glucose;

[0031] In sub-operation S122, within 2 hours from the time when the object under test is given glucose, the blood glucose value of the object under test is detected multiple times at preset detection times to obtain blood glucose values corresponding to the detection times;

[0032] Among them, the blood glucose value under the baseline conditions is used as the blood glucose value when the detection time is 0 minutes; the first blood glucose tolerance information includes the blood glucose value corresponding to the detection time.

[0033] According to an embodiment of the present invention, in sub-operation S121, the baseline condition can be a fasting state or no food intake for more than 2 hours prior to the glucose tolerance test. For example, when the test subject is a mouse, food can be removed 2 hours before the glucose tolerance test, or when the test subject is a human subject, the subject can be ensured to be fasting on the day of the test. By adjusting the test subject to the same baseline conditions, the accuracy of the glucose tolerance test can be ensured, making the results comparable to those corresponding to different light stimulation modes.

[0034] According to an embodiment of the present invention, in sub-operation S122, such as Figure 2 As shown in Figure A, taking mice as the test subject, the mice were exposed to the corresponding light stimulation mode starting at 13:00. Two hours after the preset exposure time (i.e., 15:00), glucose was administered via intraperitoneal injection (called ipGTT). 15:00 is the starting point for the detection time of 0 minutes. After that, whole blood from the tail tip was collected at 15 min, 30 min, 60 min, and 90 min and the blood glucose value was detected.

[0035] like Figure 2 As shown in Figure B, taking the test subject as an example, the subject was exposed to the corresponding light stimulation mode starting at 8:00. Two hours after the preset exposure time (i.e., 10:00), glucose was administered orally (called OGTT). 10:00 is the starting point for the detection time to be 0. Subsequently, whole blood was collected from the fingertip at 30 min, 60 min, 90 min, and 120 min and the blood glucose value was detected.

[0036] It is understandable that blood glucose testing is a common method, but it is not limited to this. In other embodiments, non-invasive testing methods such as far-infrared light measurement can be used.

[0037] According to embodiments of the present invention, such as Figure 2 As shown, in operation S120, multiple subjects are continuously exposed to the light stimulation mode until the end of the glucose tolerance test, thereby enabling the light stimulation mode to better regulate glucose metabolism.

[0038] According to an embodiment of the present invention, in order to compare the regulatory abilities of different light stimulation modes on glucose metabolism, operation S130 specifically includes sub-operations S131 to S132:

[0039] In the sub-operation S131, for each of the light stimulation modes, the blood glucose values of the plurality of test subjects at each detection time are averaged to obtain a blood glucose average value corresponding to the detection time.

[0040] In the sub-operation S132, a blood glucose metabolism curve corresponding to the light stimulation mode is plotted according to the plurality of detection times and the blood glucose average values corresponding to the detection times; and the second blood glucose tolerance information includes the blood glucose metabolism curve and an area under the blood glucose metabolism curve.

[0041] It can be understood that the manner of averaging the blood glucose values of the plurality of test subjects at each detection time may, for example, be an arithmetic mean, and the obtained arithmetic mean is the blood glucose average value.

[0042] According to an embodiment of the present application, the operation S140 specifically includes the sub-operation S141 and the sub-operation S142: in the sub-operation S141, a light stimulation mode with a lower amplitude and / or area of the blood glucose metabolism curve is determined as a target light stimulation mode from the plurality of light stimulation modes; and in the sub-operation S142, a spectral parameter corresponding to the target light stimulation mode is determined as a target spectral parameter.

[0043] For example, as shown in FIGS. 1 and 2, in the case of the test subjects being mice, the amplitudes of the blood glucose metabolism curves of the mice under the red light stimulation mode and the dark stimulation mode are lower than those under other light stimulation modes, and the areas under the blood glucose metabolism curves of the mice under the red light stimulation mode and the dark stimulation mode are lower than those under other light stimulation modes, indicating that the red light stimulation mode and the dark stimulation mode are both target light stimulation modes, and the spectral parameters corresponding to the red light stimulation mode and the dark stimulation mode are target spectral parameters. Figure 4 Figure 5 Specifically, the target spectral parameter is a spectral wavelength range of 610-630 nm and a spectral intensity of 0-7*10 3 (photons / s*um 2 ); for example, 6.62*10 3 (photons / s*um 2 ).

[0044] According to an embodiment of the present application, similar test results are obtained for mice and test subjects, i.e., the red light stimulation mode and the dark stimulation mode are more conducive to the utilization of glucose in the blood by the body than the white light stimulation mode and the blue light stimulation mode, indicating that the target spectral parameter determined by the present application is universal for different test subjects and can be applied to improve the blood glucose metabolism capacity of the human body.

[0045] ​According to embodiments of the present application, these different light stimuli patterns were further tested on how they modulate glucose tolerance, specifically by pupil light reflex test on mice with only intrinsically photosensitive ganglion cells (ipRGCs) photosensitive, as shown in Figure 3 Figure A shows the blue light spectrum used in embodiments of the present application, and the pupil light reflex test on mice with only ipRGCs photosensitive shows that blue light can effectively activate ipRGCs to cause pupil constriction. Figure B shows the red light spectrum used in embodiments of the present application, and the pupil light reflex test on mice with only ipRGCs photosensitive shows that red light cannot effectively activate ipRGCs and does not cause pupil constriction. Figure C shows the white light spectrum used in embodiments of the present application, and the pupil light reflex test on mice with only ipRGCs photosensitive shows that white light can effectively activate ipRGCs to cause pupil constriction. Figure D shows the total photon intensity of the white, blue and red light spectra and the equivalent 480 nm (the spectrum that can most effectively activate ipRGCs) light intensity, showing that the total photon intensity of the three spectra are similar. The equivalent 480 nm photon intensity of red light is 2 to 3 orders of magnitude lower than that of blue or white light. The results show that red light stimulation and dark stimulation are consistent, and cannot effectively activate the intrinsically photosensitive ganglion cells of the retina, and can increase the body's use of glucose in the blood and thus improve the body's glucose metabolism.

[0046] The technical solutions of the present application are described in detail below by listing a plurality of specific embodiments. It should be noted that the specific embodiments below are only for example and do not limit the present application. If not specified, the specific experimental conditions or methods in the following examples are carried out according to the conventional conditions or methods in the art.

[0047] Example 1: Dark stimulation improves glucose tolerance in mice and human subjects

[0048] The test subjects were SPF male C57BL / 6J mice (Sibeifu (Beijing) Biotechnology Co., Ltd.), 10 weeks old, glucose (Sigma-Aldrich); human subjects aged 20-30 years old, BMI 19-25, 50% oral glucose solution (Jitianrui); and blood glucose meter (Roche).

[0049] Implementation of light stimulation patterns: The light source for the test environment was a white LED lamp, and the spectral characteristics are shown in Figure 3 This spectrum can effectively activate ipRGCs. Two hours before the glucose tolerance test, the experimental mice or subjects were exposed to the white light or dark environment for the test, and the exposure continued until the end of the glucose tolerance test (see Figure 2 ).

[0050] Experiment 1: As shown in Figure 1 and Figure 2 , the specific experimental process corresponding to the mouse test subjects included the following steps:

[0051] a) Before the blood glucose tolerance test, multiple mice need to be adapted to the experimental room for two weeks.

[0052] b) On the experimental day, one of the light stimulation modes of the multiple mice is selected from the dark stimulation mode and the white light stimulation mode, the spectral parameters of the white light stimulation mode are as shown in the table below, and after a certain interval, for example, three or four days, the other light stimulation mode is exposed to the multiple mice. The mice are exposed to the light environment characterized by the selected light stimulation mode, and the food is removed first. Figure 3

[0053] c) Two hours later, the blood glucose tolerance test is performed, and the multiple mice are continuously exposed to the selected light environment until the end of the blood glucose tolerance test. Specifically, the tail tip is cut to collect whole blood, and the blood glucose value is detected by a blood glucose rapid detection device (Roche blood glucose meter) as the blood glucose value under the basic condition. Then, intraperitoneal injection of glucose is given at a dose of 1 g / kg, and the tail tip whole blood is collected and the blood glucose value is detected at 15 min, 30 min, 60 min, and 90 min after intraperitoneal injection of glucose, respectively. The blood glucose values obtained by the mice at the detection times of 0 min, 15 min, 30 min, 60 min, and 90 min are taken as the first blood glucose tolerance information.

[0054] d) Based on the blood glucose values of the multiple mice at different detection times under the selected light stimulation mode, the average blood glucose values corresponding to the detection times are determined, respectively, and then the relative change of the blood glucose metabolism curve is plotted according to the relative values of the average blood glucose values and the basic blood glucose values at different detection times, and the amplitude and / or the area under the curve of the blood glucose metabolism curve are taken as the second blood glucose tolerance information to characterize the blood glucose tolerance improvement ability under the selected light stimulation mode.

[0055] e) After the multiple mice complete the exposure and blood glucose tolerance test under the white light stimulation mode and the dark stimulation mode, respectively, according to the second blood glucose tolerance information of the white light stimulation mode and the dark stimulation mode, the spectral parameters corresponding to the light stimulation mode with the optimal blood glucose tolerance improvement ability are determined as the target spectral parameters.

[0056] After the test mice are managed with glucose, their blood glucose tolerance values under white light and dark stimulation modes are analyzed. Figure 4 is a schematic diagram of the results of improving the blood glucose tolerance of mice and humans under white light stimulation and dark stimulation modes in the embodiments of the present application, as shown in Figure 4 A, the blood glucose metabolism curve of the mice, the amplitude of the blood glucose metabolism under the dark stimulation mode is lower than that under the white light stimulation mode, B, the area under the blood glucose metabolism curve of the mice, the area under the blood glucose metabolism curve under the dark stimulation mode is lower than that under the white light stimulation mode, C, the basic blood glucose level of the mice, the basic blood glucose levels under the dark stimulation mode and the white light stimulation mode are similar. ​

[0057] Experiment two: as Figure 1 and Figure 2 The specific experimental process of the corresponding to-be-measured object, the test subject, includes the following steps as shown in Figure B of

[0058] a) recruit a plurality of healthy test subjects for blood glucose tolerance experiments.

[0059] b) on the experimental day, the test subjects need to be on an empty stomach, and one of the dark stimulation mode and the white light stimulation mode is selected for a plurality of test subjects. The spectral parameters of the white light stimulation mode are as shown in Figure 3 and after a certain interval of time, for example, three to seven days, another light stimulation mode is exposed to the plurality of test subjects. The test subjects comfortably sit on a stool in the experimental room and are exposed to the light environment represented by the selected light stimulation mode, remaining quiet.

[0060] c) two hours later, the blood glucose tolerance test is performed, and the plurality of test subjects are continuously exposed to the selected light environment until the blood glucose tolerance test is completed. Specifically, fingertip whole blood is collected, and the blood glucose value at this time is detected using a blood glucose rapid detection device (Roche blood glucose meter) as the blood glucose value under the basic condition. Then, a glucose solution is orally administered, with a dose of 75g / person, and a 50% glucose original solution of 150ml is diluted to 300ml with warm water. Fingertip whole blood is collected and the blood glucose value is detected at 30min, 60min, 90min, and 120min after oral administration of glucose, respectively. The blood glucose values obtained by the test subjects at detection times of 0min, 30min, 60min, 90min, and 120min are taken as the first blood glucose tolerance information.

[0061] d) under the selected light stimulation mode, the blood glucose average values corresponding to different detection times are determined based on the blood glucose values of the plurality of test subjects at different detection times, and then the relative change in blood glucose metabolism curve is plotted according to the relative values of the blood glucose average values at different detection times and the base blood glucose value, and the amplitude of the blood glucose metabolism curve and / or the area under the curve are taken as the second blood glucose tolerance information to represent the blood glucose tolerance improvement ability under different light stimulation modes.

[0062] e) after the plurality of test subjects complete exposure and blood glucose tolerance tests under the white light stimulation mode and the dark stimulation mode, respectively, the second blood glucose tolerance information of the white light stimulation mode and the dark stimulation mode is used to determine the spectral parameters corresponding to the light stimulation mode with the optimal blood glucose tolerance improvement ability as the target spectral parameters.

[0063] After the test subjects are administered glucose, their blood glucose tolerance values under white light and dark stimulation modes are analyzed. As shown in Figure 4As shown in Figure D, the blood glucose metabolism curve of the subjects is shown. The amplitude of blood glucose metabolism under the dark stimulation mode is lower than that under the white light stimulation mode. Figure E shows the area under the blood glucose metabolism curve of the subjects. The area under the blood glucose metabolism curve under the dark stimulation mode is lower than that under the white light stimulation mode. Figure F shows the basal blood glucose level of the subjects. The basal blood glucose level is similar under the dark stimulation mode and the white light stimulation mode.

[0064] The results above show that dark stimulation can improve the glucose metabolism of subjects and mice.

[0065] Example 2: Red light stimulation improves glucose tolerance in mice.

[0066] The subjects were: SPF-grade male C57BL / 6J mice (Speford (Beijing) Biotechnology Co., Ltd.), 10 weeks old, glucose (Sigma-Aldrich), blood glucose meter (Roche); subjects aged 20-30 years, BMI 19-25, 50% oral glucose solution (Gilead), blood glucose meter (Roche).

[0067] Implementation of the photostimulation mode: The ambient light source used in the test was an LED lamp with spectral characteristics of blue light and red light. The blue light spectrum effectively activated ipRGCs, but the red light spectrum could not activate ipRGCs (see...). Figure 3 Two hours prior to the glucose tolerance test, the experimental mice or subjects were exposed to the test light environment, and this exposure continued until the end of the glucose tolerance test (see [link to test instructions]). Figure 2 ).

[0068] In this embodiment, the specific experimental process for the test object is similar to that in Embodiment 1, the only difference being that the multiple light stimulation modes are red light, blue light and dark stimulation modes.

[0069] After glucose management in test mice and humans, their glucose tolerance values ​​under different spectra were analyzed. Figure 5 This is a schematic diagram illustrating the results of improving glucose tolerance in mice under different light stimulation modes in embodiments of the present invention; as shown. Figure 5 As shown, Figures A to C represent the glucose metabolism curves of mice under dark stimulation, blue light stimulation, and red light stimulation, respectively. The amplitude of the glucose metabolism curve of mice under red light stimulation is lower than that under other light stimulation modes. Figure D shows the area under the glucose metabolism curve of mice. The area under the glucose metabolism curve of mice under red light and dark stimulation modes is lower than that under blue light conditions. Figure E shows that the basal blood glucose levels are similar under dark stimulation, red light stimulation, and blue light stimulation modes.

[0070] Figure 6 This is a schematic diagram illustrating the results of improving human glucose tolerance under different light stimulation modes in embodiments of the present invention. For example... Figure 6As shown, A to C are blood glucose metabolism curves of the subjects under dark stimulation mode, blue light stimulation mode and red light stimulation mode, respectively, the amplitudes of the blood glucose metabolism curves under red light stimulation mode and dark stimulation mode are lower than that under blue light stimulation mode, D is the area under the curve of the blood glucose metabolism of the subjects, the area under the curve of the blood glucose metabolism of the mice under red light and dark stimulation mode is lower than that under blue light condition, E shows that the basal blood glucose levels under dark stimulation mode, red light stimulation mode and blue light stimulation mode are similar.

[0071] The above results show that the red light stimulation mode and the dark stimulation mode which cannot activate ipRGCs can improve the blood glucose metabolism capacity of the mice and the subjects.

[0072] In summary, the blood glucose regulation effect of light can be used as a non-invasive means for treating sugar metabolism related diseases, and has the advantages of safety, effectiveness and low side effects. When the body is responding to energy challenges (such as eating), by adjusting the wavelength and intensity of the light used for lighting in the environment, the self-photoreceptor ganglion cells in the retina are stimulated as little as possible, thereby promoting the body's use of glucose in the blood and improving the ability of sugar metabolism, and thus can be used for treating sugar metabolism related diseases and preventing the occurrence of related diseases. At present, the biological regulation of light is mainly used for the treatment of neonatal jaundice, skin diseases and mental diseases, and there is no treatment and prevention of sugar metabolism diseases. Therefore, the present application provides a new strategy for improving the blood glucose metabolism capacity, i.e. the red light stimulation mode increases the body's use of blood glucose when the body is responding to energy challenges, and improves the body's blood glucose metabolism capacity.

[0073] The above specific embodiments further illustrate the purpose, technical solutions and beneficial effects of the present application, and it should be understood that the above description is only for specific embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A method for determining spectral parameters suitable for regulating glucose metabolism based on the spectral sensitivity of self-sensing photoganglionic cells, comprising the following steps: Each of the multiple test subjects was exposed to multiple light stimulation modes, wherein the multiple light stimulation modes included a stimulation mode of blue light, red light and dark light or a stimulation mode of white light and dark light, and the test subjects were mice or subjects with photosensitive ganglion cells. For each of the light stimulation modes, after a preset exposure time, the multiple test subjects are subjected to a glucose tolerance test to obtain the first glucose tolerance information of the multiple test subjects. The first glucose tolerance information is used to characterize the glucose tolerance ability of the test subjects. The multiple test subjects are continuously exposed to the light stimulation mode until the glucose tolerance test ends. Based on the first glucose tolerance information of the plurality of subjects to be tested, second glucose tolerance information corresponding to the plurality of light stimulation modes is determined, wherein the second glucose tolerance information is used to characterize the glucose tolerance improvement ability of the light stimulation mode, and the second glucose tolerance information includes a glucose metabolism curve and the area under the glucose metabolism curve. Based on the second glucose tolerance information of the multiple light stimulation modes, the spectral parameters corresponding to the light stimulation mode with the best glucose tolerance improvement ability are determined as target spectral parameters, including: determining the light stimulation mode with a lower amplitude and / or area of ​​the glucose metabolism curve from the multiple light stimulation modes as the target light stimulation mode. And the spectral parameters corresponding to the target light stimulation mode are determined as the target spectral parameters.

2. The method for determining spectral parameters according to claim 1, characterized in that, The preset exposure time is 2 to 3 hours.

3. The method for determining spectral parameters according to claim 1, characterized in that, The spectral parameters include the spectral wavelength range and spectral intensity.

4. The method for determining spectral parameters according to claim 1, characterized in that, The step of performing a glucose tolerance test on the multiple test subjects after a preset exposure time to obtain the first glucose tolerance information of the multiple test subjects includes: After detecting the blood glucose level of the subject under basal conditions, glucose was administered to the subject. Within 2 hours from when glucose is administered to the subject, the blood glucose level of the subject is measured multiple times at multiple preset detection times to obtain the blood glucose value corresponding to the detection time. The blood glucose value under the aforementioned baseline conditions is used as the blood glucose value at a detection time of 0 minutes; the first blood glucose tolerance information includes the blood glucose value corresponding to the detection time.

5. The method for determining spectral parameters according to claim 4, characterized in that, The baseline condition is being in a fasting state or not eating for more than 2 hours before the glucose tolerance test.

6. The method for determining spectral parameters according to claim 4, characterized in that, The step of determining the second glucose tolerance information corresponding to each of the multiple light stimulation modes based on the first glucose tolerance information of the multiple test subjects includes: For each of the light stimulation modes, the blood glucose values ​​of the multiple subjects to be tested are averaged at each detection time to obtain the average blood glucose value corresponding to the detection time; Based on multiple detection times and the average blood glucose value corresponding to the detection time, a blood glucose metabolism curve corresponding to the light stimulation mode is plotted.

7. The method for determining spectral parameters according to claim 1, characterized in that, The target spectral parameters include a spectral wavelength range of 610~630 nm and a spectral intensity equivalent to a 480 nm spectrum of 0~7*10. 3 (Number of photons / s*um) 2 ).

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