An apparatus and method for batch surface treatment and zonal functionalization of imaging chips

Abstract

The present invention discloses a device and method for batch surface treatment and zonal functionalization of imaging chips. The device includes a multi-channel bearing tube, a multi-functional upper cover, a variable-volume cavity, a chip sealing plate, a reaction tank sealing plate, and a number of reaction tanks. The multi-channel bearing tube is used to connect a number of the reaction tanks in series and perform the transmission and distribution of fluid samples; the reaction tank is used to batch load imaging chips and provide the driving force for chip rotation through its own turbine structure; the multi-functional upper cover is arranged above the variable-volume cavity and is used to realize the automatic loading and unloading of fluid samples; the variable-volume cavity is used to load the reaction tanks, and its volume adapts to different numbers of reaction tanks to reduce the usage amount of fluid samples; the chip sealing plate is arranged in the reaction tank and is used to realize self-zoning of batch imaging chips; the reaction tank sealing plate is arranged between every two reaction tanks and is used to seal adjacent reaction tanks, and cooperate with the multi-channel bearing tube to realize zonal functionalization of chips in different reaction tanks.

Description

Technical Field

[0001] This application relates to the technical field of chip processing devices, and particularly to a device and method for batch surface treatment and zone functionalization of imaging chips. Background Art

[0002] Most traditional biochemical and molecular analysis methods detect the average signals of biological populations, often ignoring the heterogeneity of single molecules in the entire microenvironment. Optical imaging has shown great prowess in the field of single molecule detection due to its high sensitivity and visualization characteristics, and is widely used in fields such as disease diagnosis, environmental monitoring, new drug research and development, and life sciences.

[0003] As the basis for the optical imaging effect, the surface treatment and functionalization of imaging chips are important links in the detection process. Existing imaging chip surface treatments require multiple reagents to remove surface impurities; parameters such as the types and concentrations of chip functionalization reagents often need to be explored one by one to find the optimal conditions. However, most chip surface treatments require manual single-chip operations, which are time-consuming and laborious, and are prone to introducing impurities or damage, making it difficult to ensure the uniformity between different chips and affecting the detection effect. There are also few automatic devices for batch processing of chip surface functionalization in different regions. Manual operations will inevitably introduce errors and are difficult to meet the requirements of high-efficiency imaging. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the purpose of the embodiments of this application is to provide a device and method for batch surface treatment and zone functionalization of imaging chips.

[0005] According to the first aspect of the embodiments of this application, a device for batch surface treatment and zone functionalization of imaging chips is provided, including a multi-channel bearing tube, a multi-functional upper cover, a variable-volume cavity, a chip sealing plate, a reaction tank sealing plate, and a number of reaction tanks.

[0006] The multi-channel bearing tube is used to connect a number of the reaction tanks in series and perform the transmission and distribution of fluid samples. The fluid samples are fluids that can be used for the treatment and functionalization of imaging chips, including chemical reagents, biological samples, and high-purity gases.

[0007] The reaction tanks are used to batch load imaging chips and provide the driving force for chip rotation through their own turbine structures. The imaging chips are glass slides, ITO slides, gold slides, and other chips used for optical imaging.

[0008] The multi-functional upper cover is arranged above the variable-volume cavity to achieve the automatic loading and unloading of the fluid samples.

[0009] The variable-volume cavity is used to load the reaction tanks, and its volume adapts to different numbers of reaction tanks to reduce the usage of fluid samples.

[0010] The chip sealing plate is arranged in the reaction tank to achieve self-partitioning of the batch imaging chips and realize surface treatment or functionalization of different regions;

[0011] The reaction tank sealing plate is arranged between every two reaction tanks to seal adjacent reaction tanks and cooperate with the multi-channel bearing tube to realize chip partitioning and functionalization of different reaction tanks.

[0012] Further, the multi-channel bearing tube includes an exhaust pipe and a chassis. The exhaust pipe is arranged on the chassis through a smooth bearing. A number of circumferential exhaust holes are arranged on the body of the exhaust pipe. The number of the circumferential exhaust holes is the same as the number of the reaction tanks and the circumferential exhaust holes are in one-to-one correspondence and communication with the reaction tanks. A partitioned sample inlet is arranged at the upper end of the exhaust pipe, and a number of pipelines are arranged on the partitioned sample inlet to respectively communicate with the circumferential exhaust holes.

[0013] Further, the reaction tank includes an upper reaction tank and a lower reaction tank. The upper reaction tank includes an upper chip slot, an upper bearing hole, an upper buckle, and an upper smooth bearing. The lower reaction tank includes a lower chip slot, a water drainage hole, a lower bearing hole, and a lower buckle. The upper reaction tank and the lower reaction tank are connected in series by using the multi-channel bearing tube through the upper bearing hole and the lower bearing hole. The upper chip slot and the lower chip slot are symmetric up and down. The upper smooth bearing is arranged at the top of the upper reaction tank to reduce the friction caused by the rotation of the reaction tank. The upper buckle and the lower buckle cooperate with each other to make the upper reaction tank and the lower reaction tank fit tightly. The water drainage hole is arranged at the bottom of the lower reaction tank to prevent the fluid sample from remaining.

[0014] Further, the planar shapes of the upper chip slot and the lower chip slot are trapezoids, and the outer side length is 1 / 2 - 2 / 3 of the inner side length.

[0015] Further, the multifunctional upper cover includes a timer, multi-channel pipelines, a partition board, a cover board, a sample inlet, a driving pump, and a power supply. The driving pump and the multi-channel pipelines are used to realize the inlet and outlet and switching of various fluid samples. The timer is used to record the processing time. The partition board separates the driving pump and the power supply for waterproofing the power supply. The cover board is used to close the multifunctional upper cover. The cover board is provided with the sample inlet to connect the multi-channel pipelines and the partitioned sample inlet on the multi-channel bearing tube.

[0016] Further, a sealed piston is arranged in the variable-volume cavity. The height of the sealed piston can be adjusted according to the number of reaction tanks to change the cavity volume, so as to reduce the usage amount of the fluid sample. A groove slope is arranged at the bottom of the variable-volume cavity to prevent the generation of fluid dead volume from affecting the processing effect. A sample outlet is arranged at the bottom of the side wall to realize the discharge of the fluid sample or to connect with the sample inlet to realize circular flow.

[0017] Further, the chip sealing plate includes a chip sealing card slot and a bearing tube sealing sleeve. The chip sealing card slot is used to seal the chip, and the bearing tube sealing sleeve is used to close the redundant surrounding exhaust holes.

[0018] According to the second aspect of the embodiments of the present application, a method for batch surface treatment and zone functionalization of imaging chips is provided. The method is applied to the device described in the first aspect and includes the following steps:

[0019] Step 1: Sequentially place the imaging chips into the reaction tank and clamp and fix them.

[0020] Step 2: Snap in the chip sealing plate according to the zoning requirements, pass the multi-channel bearing tube through several of the reaction tanks from bottom to top, and insert a reaction tank sealing plate between every two adjacent reaction tanks.

[0021] Step 3: Place the assembled reaction tank and the multi-channel bearing tube into the variable-volume cavity, and slowly rotate the sealed piston in the variable-volume cavity along the multi-channel bearing tube to the surface of the uppermost reaction tank.

[0022] Step 4: Cover the multi-functional upper cover, turn on the power supply, turn on the drive pump, and inject multiple fluid samples into different pipelines of the multi-channel bearing tube through the sample inlet respectively, so as to drive the imaging chips and the reaction tank to rotate through the fluid samples.

[0023] Step 5: After the fluid samples are processed, open the multi-functional upper cover, remove the variable-volume cavity, inject high-purity gas along the multi-channel bearing tube, and perform high-efficiency drying treatment using rotational centrifugal force.

[0024] Further, Steps 1 to 5 are specifically as follows:

[0025] Step 1: Sequentially place the imaging chips into the lower chip card slots of the lower reaction tank, and place and clamp and fix the imaging chips on the side close to the narrow side of the chip card slot.

[0026] Step 2: Snap the chips into the chip sealing plate one by one along the chip sealing card slot, twist and fasten the upper buckle of the upper reaction tank and the lower buckle of the lower reaction tank, pass the multi-channel bearing tube through the lower bearing hole in the lower reaction tank and the upper bearing hole in the upper reaction tank from bottom to top, and repeat the above operations as needed for the installation of the remaining reaction tanks. Insert a reaction tank sealing plate between every two reaction tanks to separate the reaction tanks.

[0027] Step 3: Place the assembled reaction tank into the variable-volume cavity, and slowly rotate the sealed piston along the multi-channel bearing tube to the surface of the uppermost reaction tank.

[0028] Step 4: Cover the multi-functional upper cover, turn on the power supply, turn on the drive pump, and inject multiple fluid samples into different pipelines of the multi-channel bearing pipe through the sampling port respectively to realize the processing of different fluid samples in different reaction tanks. When injecting the samples, the imaging chip rotates clockwise, and when changing the direction of the drive pump for sample suction, the chip rotates counterclockwise. The imaging chip drives the reaction tank to rotate;

[0029] Step 5: After the fluid sample processing is completed, open the multi-functional upper cover, remove the variable-volume cavity, inject high-purity gas along the multi-channel bearing pipe, and perform drying treatment using rotational centrifugal force.

[0030] The technical solutions provided by the embodiments of the present application may include the following beneficial effects:

[0031] (1) Each reaction tank of the imaging chip surface treatment device described in this invention patent has 20 - 50 chip slots, which can perform large-scale imaging chip surface treatment, improving the processing efficiency. According to the required number of reaction tanks, the variable-volume cavity can be used to reduce the usage of fluid samples and lower the cost;

[0032] (2) The imaging chip surface treatment device described in this invention patent can achieve the zonal functionalization of chips in different reaction tanks and the zonal functionalization of batch single chips, improving the efficiency of exploring functionalization parameters in experiments;

[0033] (3) The method of using the imaging chip surface treatment device described in this invention patent can use the drive pump to realize the low-speed clockwise and counterclockwise rotation switching of the imaging chip along with the flow of the fluid sample, playing a role in stirring and mixing, accelerating the reaction rate, and improving the efficiency of chip surface treatment and functionalization.

[0034] It should be understood that the above general description and the following detailed description are only exemplary and explanatory, and cannot limit the present application. Description of the Drawings

[0035] The drawings here are incorporated into the specification and constitute a part of this specification, showing the embodiments consistent with the present application, and are used together with the specification to explain the principles of the present application.

[0036] Figure 1 It is a schematic assembly structure diagram of the reaction tank described in the embodiment of this invention patent;

[0037] Figure 2 It is an exploded schematic assembly structure diagram of the reaction tank described in the embodiment of this invention patent;

[0038] Figure 3 It is a rotating exploded schematic assembly structure diagram of the reaction tank described in the embodiment of this invention patent;

[0039] Figure 4Schematic diagram of the multi-channel bearing tube structure according to the embodiment of the present invention patent;

[0040] Figure 5 Top view schematic diagram of the multi-channel bearing tube according to the embodiment of the present invention patent;

[0041] Figure 6 Cross-sectional schematic diagram of the multi-channel bearing tube according to the embodiment of the present invention patent;

[0042] Figure 7 First perspective structure schematic diagram of the upper reaction tank according to the embodiment of the present invention patent;

[0043] Figure 8 Second perspective structure schematic diagram of the upper reaction tank according to the embodiment of the present invention patent;

[0044] Figure 9 Bottom view schematic diagram of the upper reaction tank according to the embodiment of the present invention patent;

[0045] Figure 10 First perspective structure schematic diagram of the lower reaction tank according to the embodiment of the present invention patent;

[0046] Figure 11 Second perspective structure schematic diagram of the lower reaction tank according to the embodiment of the present invention patent;

[0047] Figure 12 Top view schematic diagram of the multi-functional upper cover according to the embodiment of the present invention patent;

[0048] Figure 13 Bottom view schematic diagram of the multi-functional upper cover according to the embodiment of the present invention patent;

[0049] Figure 14 Top view schematic diagram of the volume-variable cavity according to the embodiment of the present invention patent;

[0050] Figure 15 Cross-sectional schematic diagram of the volume-variable cavity according to the embodiment of the present invention patent;

[0051] Figure 16 Top view schematic diagram of the chip sealing plate according to the embodiment of the present invention patent;

[0052] Figure 17 Exploded structure schematic diagram of the imaging chip batch surface treatment and partition functionalization device according to the embodiment of the present invention;

[0053] Figure 18 Schematic diagram of the imaging chip batch surface treatment and partition functionalization device according to the embodiment of the present invention.

[0054] Explanation of reference numerals:

[0055] 1. Multi-channel bearing tube; 101. Exhaust pipe; 102. Partitioned sampling port; 103. Upper circumferential exhaust hole; 104. Middle circumferential exhaust hole; 105. Lower circumferential exhaust hole; 106. Smooth bearing; 107. Drain hole; 108. Chassis; 2. Upper reaction tank; 201. Upper chip card slot; 202. Upper bearing hole; 203. Upper buckle; 204. Upper smooth bearing; 3. Lower reaction tank; 301. Lower chip card slot; 302. Drainage hole; 303. Lower bearing hole; 304. Lower buckle; 4. Imaging chip; 5. Multifunctional upper cover; 501. Timer; 502. Multi-channel pipeline; 503. Partition board; 504. Cover plate; 505. Sampling port; 506. Driving pump; 507. Power supply; 6. Variable-volume cavity; 601. Groove slope; 602. Sampling outlet; 603. Sealed piston; 7. Chip sealing plate; 701. Chip sealing card slot; 702. Bearing tube sealing sleeve; 8. Reaction tank sealing plate. Detailed implementation manners

[0056] Here, exemplary embodiments will be described in detail, and examples thereof are shown in the drawings. When the following description refers to the drawings, unless otherwise indicated, the same numbers in different drawings represent the same or similar elements. The implementation manners described in the following exemplary embodiments do not represent all implementation manners consistent with the present application.

[0057] The terms used in the present application are only for the purpose of describing specific embodiments and are not intended to limit the present application. The singular forms "a", "the", and "said" used in the present application and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. It should also be understood that the term "and / or" used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0058] It should be understood that although the terms first, second, third, etc. may be used in the present application to describe various information, such information should not be limited to these terms. These terms are only used to distinguish the same type of information from each other. For example, without departing from the scope of the present application, the first information may also be referred to as the second information, and similarly, the second information may also be referred to as the first information. Depending on the context, the word "if" as used herein may be interpreted as "when" or "while" or "in response to determining".

[0059] Such as Figure 1 , Figure 2 and Figure 3As shown in the figure, the present application provides a device for batch surface treatment and zonal functionalization of imaging chips 4 using fluid samples. The device may include a multi-channel bearing tube 1, a multi-functional upper cover 5, a variable-volume cavity 6, a chip sealing plate 7, a reaction tank sealing plate 8, and a number of reaction tanks. The multi-channel bearing tube 1 is used to connect a number of the reaction tanks in series and transmit and distribute fluid samples; the reaction tanks are used to batch-load imaging chips 4 and provide the driving force for chip rotation through their own turbine structures; the multi-functional upper cover 5 is arranged above the variable-volume cavity 6 to realize the automatic loading and unloading of the fluid samples; the variable-volume cavity 6 is used to load the reaction tanks, and its volume adapts to different numbers of reaction tanks to reduce the amount of fluid samples used; the chip sealing plate 7 is arranged in the reaction tanks to realize self-zoning of the batch imaging chips 4 and achieve surface treatment or functionalization of different regions; the reaction tank sealing plate 8 is arranged between every two reaction tanks to seal adjacent reaction tanks and cooperate with the multi-channel bearing tube 1 to realize zonal functionalization of chips in different reaction tanks.

[0060] As can be seen from the above embodiments, the reaction tanks of the present application can batch-load imaging chips 4, solving the technical problem of low processing efficiency of imaging chips 4 and enabling batch surface treatment of a large number of imaging chips 4. According to the required number of reaction tanks, the variable-volume cavity 6 can be used to reduce the amount of fluid samples used, solving the technical problem of waste of fluid samples and reducing costs; zonal functionalization of chips in different reaction tanks and zonal functionalization of batch single chips can be realized, solving the technical problem of single functionalization of imaging chips 4 and improving the efficiency of exploration experiments on functionalization parameters.

[0061] Specifically, the imaging chip 4 is a glass slide, an ITO glass slide, a gold chip, and other chips for optical imaging.

[0062] Specifically, the fluid sample is a fluid that can be used for chip treatment and functionalization, including chemical reagents, biological samples, and high-purity gases.

[0063] Such as Figure 4 、 Figure 5 and Figure 6 As shown in

[0064] Such asFigure 7 , Figure 8 , Figure 9 , Figure 10 and Figure 11 As shown in Figure 10 and Figure 11 , specifically, the reaction tank includes an upper reaction tank 2 and a lower reaction tank 3. The upper reaction tank 2 includes an upper chip card slot 201, an upper bearing hole 202, an upper buckle 203, and an upper smooth bearing 204. The lower reaction tank 3 includes a lower chip card slot 301, a water drainage hole 302, a lower bearing hole 303, and a lower buckle 304. The upper reaction tank 2 and the lower reaction tank 3 are serially combined by using the multi-channel bearing tube 1 through the upper bearing hole 202 and the lower bearing hole 303. The upper chip card slot 201 and the lower chip card slot 301 are symmetrically arranged up and down. The upper smooth bearing 204 is arranged at the top of the upper reaction tank 2 to reduce the friction caused by the rotation of the reaction tank. The upper buckle 203 and the lower buckle 304 cooperate with each other to make the upper reaction tank 2 and the lower reaction tank 3 fit tightly, which is convenient for assembly and disassembly, and can prevent the imaging chip 4 from being broken during the rotation of the reaction tank. The water drainage hole 302 is arranged at the bottom of the lower reaction tank 3 to prevent the fluid sample from remaining.

[0065] Specifically, 20 - 50 pairs of upper chip card slots 201 and lower chip card slots 301 are arranged in a single reaction tank, and are distributed in a turbine dynamics around the exhaust pipe 101. The driving pump 506 drives the reaction tank to achieve a turbine rotation effect, and the reaction tank can switch the clockwise and counterclockwise rotation directions according to the flow direction of the driving pump 506 to accelerate the reaction rate or the cleaning rate.

[0066] In a specific implementation, the (longitudinal) depth of the upper chip card slot 201 and the lower chip card slot 301 is 2 - 5 mm, which meets the requirements for the fixation and surface treatment area of the imaging chip 4. The (lateral) depth from the outer edge of the chip card slot to the edge of the reaction tank is 2 - 5 mm, which improves the limiting effect and realizes the stable fixation of the imaging chip 4. Preferably, the chip card slot is subjected to a rounded and smooth treatment to prevent the chip from being scratched during rotation.

[0067] Specifically, the planar shapes of the upper chip card slot 201 and the lower chip card slot 301 are trapezoidal, and the outer side length is 1 / 2 - 2 / 3 of the inner side length. The outer short side can fix the chip by using the rotational centrifugal force, and the inner long side is convenient for transferring the chip with fixtures such as tweezers.

[0068] In a specific implementation, the chip card slot rotates 20 - 30 degrees clockwise along the plane axis direction, which can utilize the force difference generated by the inlet and outlet flow of the fluid sample to drive the reaction tank to rotate slowly clockwise or counterclockwise, playing a role in stirring and mixing, and accelerating the reaction or cleaning rate. At the same time, when ventilating, the liquid is centrifuged and dried under the action of inertial centrifugal force, accelerating the drying rate of the chip.

[0069] As Figure 12 andFigure 13 As shown, specifically, the multifunctional upper cover 5 includes a timer 501, a multi-channel pipeline 502, a partition 503, a cover plate 504, a sample inlet 505, a driving pump 506, and a power supply 507. The inlet and outlet and switching of multiple fluid samples are realized through the driving pump 506 and the multi-channel pipeline 502. The timer 501 is used to record the processing time. The partition 503 is used to separate the power supply 507 and the driving pump 506 for waterproofing the power supply 507 and its attached wire / circuit structure. The cover plate 504 is used to seal the multifunctional upper cover 5. The cover plate 504 is provided with the sample inlet 505 to connect the multi-channel pipeline 502 and the partitioned sample inlet 505 on the multi-channel bearing tube 1. In a specific implementation, the multi-channel pipeline 502 can be externally connected to 2-5 fluid samples.

[0070] As Figure 14 and Figure 15 As shown, specifically, the variable-volume cavity 6 includes a groove slope 601, a sample outlet 602, and a sealed piston 603. The variable-volume cavity 6 can adjust the height of the closing piston and determine the height (cavity volume) according to the number of reaction cells, so as to load the reaction cells and reduce the usage amount of fluid samples. The bottom of the variable-volume cavity 6 is provided with a groove slope 601 to avoid dead volume and prevent fluid sample residue.

[0071] During fluid injection, the power of the driving pump 506 can be adjusted in a timely manner to control the flow rate to avoid the rotation speed of the reaction cell being greater than 100 RPM. After fluid injection, sample suction can be performed again to invert the reaction cell to make the surface treatment or functionalization more complete. The driving pump 506 is used to realize the low-speed clockwise and counterclockwise rotation switching of the imaging chip 4 along with the flow of the fluid sample, playing a role in stirring and mixing, accelerating the reaction rate, solving the problem of low efficiency of traditional static processing, and improving the efficiency of chip surface treatment and functionalization. Among them, for relatively expensive samples, an external pipeline can be connected to the sample inlet and outlet 602 to realize circulating flow for sample injection and extraction.

[0072] As Figure 16 As shown, in a specific implementation, the chip sealing plate 7 is snapped into the chip. The chip sealing plate 7 includes a chip sealing card slot 701 and a bearing tube sealing sleeve 702. The chip sealing card slot 701 is used to seal the chip, and the bearing tube sealing sleeve 702 is used to seal the redundant surrounding exhaust holes, and batch single-chip partitioned surface treatment or functionalization can be realized. The reaction cell sealing plate 8 is used to separate the reaction cells, cooperate with the multi-channel bearing tube 1 to introduce different fluid samples, and realize the functionalization of different reaction cell chips in partitions.

[0073] In a specific implementation, sealing material coatings are provided at the edge assembly of the variable-volume cavity 6, the chip sealing plate 7, and the reaction cell sealing plate 8 to prevent liquid leakage.

[0074] In specific implementation, the whole device is made of materials such as stainless steel that do not react with fluid samples, and the surface of the whole device is treated with surfactant for hydrophobic treatment to prevent liquid residue.

[0075] The working principle of the device for batch surface treatment and zonal functionalization of the imaging chip 4 using fluid samples is as follows:

[0076] Select the corresponding number of upper reaction tanks 2 and lower reaction tanks 3 as needed. Place the imaging chip 4 into the lower chip slot 301 in the lower reaction tank 3, and snap it into the chip sealing slot 701 in the chip sealing plate 7 as needed for zonal functionalization of the chip. Snap the upper chip slot 201 in the upper reaction tank 2 onto the corresponding imaging chip 4. The upper snap 203 in the upper reaction tank 2 and the lower snap 304 in the lower reaction tank 3 are combined and fixed to prevent the imaging chip 4 from breaking during rotation. The upper smooth bearing 106 in the upper reaction tank 2 can reduce the rotational friction, and the drain hole 302 in the lower reaction tank 3 can prevent fluid sample residue.

[0077] Snap the assembled upper reaction tank 2 and lower reaction tank 3 into the exhaust pipe 101 of the multi-channel bearing pipe 1 along the upper bearing hole 202 and the lower bearing hole 303 respectively. The exhaust pipe 101 is provided with circumferential exhaust holes located in the upper-middle parts of the corresponding reaction tanks. Place a reaction tank sealing plate 8 between every two reaction tanks for zonal functionalization of different reaction tanks. The multi-channel bearing pipe 1 is provided with zonal sample inlets 505 for transporting fluid samples to different circumferential exhaust holes, and is provided with a chassis 108 for supporting the reaction tanks. The chassis 108 is provided with drain holes 107 to prevent fluid sample residue, and is provided with smooth bearings 106 to reduce rotational friction.

[0078] Place the assembled multi-channel bearing pipe 1 and several upper reaction tanks 2 and lower reaction tanks 3 into the variable-volume cavity 6. Adjust the height of the sealed piston 603 to reduce the amount of fluid sample used. Connect the required fluid sample to the multi-channel pipeline 502 on the multi-functional upper cover 5 (complete the overall assembly of the device, and the finally formed device is as shown in Figure 17 and Figure 18 ). The drive pump 506 in the multi-functional upper cover 5 injects the fluid samples into the corresponding reaction tanks through different zonal sample inlets 505 in the multi-channel bearing pipe 1 respectively. The bottom of the variable-volume cavity 6 is provided with a groove slope 601 to prevent the formation of dead volume at the bottom of the fluid sample, and is provided with a sample outlet 602 for sample discharge. The imaging chip 4 is distributed in a turbine dynamics manner in the reaction tank, and the injection of the fluid sample drives the imaging chip 4 to rotate, accelerating the reaction rate and making the reaction more complete.

[0079] After the treatment is completed, introduce high-purity gas, and use centrifugal force for rapid drying when the imaging chip 4 rotates. The top of the multi-functional upper cover 5 is provided with a timer 501 to record the treatment time. In this way, batch surface treatment and zonal functionalization of the imaging chip 4 are realized.

[0080] The following is an exemplary application of this device, an experiment to explore the batch surface treatment and zonal functionalization parameters of a possible surface plasmon resonance imaging chip 4. The specific steps are as follows:

[0081] (1) Slide surface treatment. First, place the slide into the surface treatment device. Place the treatment device in an ultrasonic cleaner and turn on the maximum power. Use the drive pump 506 to continuously introduce acetone, absolute ethanol, and ultrapure water solution into the multi-way bearing tube 1 for 3 minutes each in sequence. Then, change the direction of the drive pump 506 and aspirate the liquid in the device for 3 minutes to reverse the reaction tank. The sample flows directly into the beaker through the sample outlet 602. After each liquid treatment of the chip, open the multi-functional upper cover 5, remove the variable-volume cavity 6, and introduce high-purity nitrogen gas through the injection port 505 for 2 minutes.

[0082] (2) Preparation of the imaging chip 4. Then, take out the slide with tweezers and perform controlled sputtering physical vapor deposition with parameters of 2 nm chromium and 48 nm gold to prepare the imaging chip 4. Then, quickly polish the imaging chip 4 in a hydrogen flame at 75 L / min for 5 seconds.

[0083] (3) Surface treatment of the imaging chip 4. Then, place the treatment device in an ultrasonic cleaner and turn on the maximum power. Use the drive pump 506 to continuously introduce acetone, absolute ethanol, and ultrapure water solution into the multi-way bearing tube 1 for 3 minutes. Then, change the direction of the drive pump 506 and aspirate the liquid in the device for 3 minutes to reverse the reaction tank. The sample flows directly into the beaker through the sample outlet 602. After each liquid treatment of the chip, open the multi-functional upper cover 5, remove the variable-volume cavity 6, and introduce high-purity nitrogen gas through the injection port 505 for 2 minutes. Take out the device and treat the imaging chip 4 in a plasma cleaner at a power of 80 W for 5 minutes.

[0084] (4) Zonal functionalization of the imaging chip 4. Reload the imaging chip 4. Insert the chip sealing plate 7 into the upper reaction tank. Do not functionalize the upper part of the upper imaging chip 4, which is used as a blank functionalization control. Insert the reaction tank sealing plate 8 into the middle of the reaction tank. Use the drive pump 506 to introduce 0.1 mM, 1 mM, and 10 mM SH-PEG-Biotin solutions into the three reaction tanks through the multi-way bearing tube 1 for 15 minutes respectively. Then, change the direction of the drive pump 506 and aspirate the liquid in the device for 10 minutes to reverse the reaction tank and complete the surface functionalization of the imaging chip 4. Since the cost of biological samples is relatively high, connect the sample outlet 602 to the injection port 505 for circulating flow in and out of the sample. After each liquid treatment of the imaging chip 4, open the multi-functional upper cover 5, remove the variable-volume cavity 6, and introduce high-purity nitrogen gas through the multi-way bearing tube 1 for 2 minutes. When injecting the fluid, adjust the power of the drive pump 506 in a timely manner to control the flow rate and avoid the rotation speed of the reaction tank being greater than 100 RPM. In this way, the experiment to explore different functionalization parameters is completed.

[0085] The present application also provides a method for batch surface treatment and zonal functionalization of an imaging chip 4 using a fluid sample, and the method may include the following steps:

[0086] Step 1: Sequentially place the imaging chip 4 into the reaction tank and clamp and fix it.

[0087] Step 2: Snap the chip sealing plate 7 according to the zonal requirements, pass the multi-way bearing tube 1 from bottom to top through a plurality of the reaction tanks, and insert a reaction tank sealing plate 8 between two adjacent reaction tanks.

[0088] Step 3: Place the assembled reaction tank and the multi-way bearing tube 1 into the variable-volume cavity 6, and slowly rotate the sealed piston 603 in the variable-volume cavity 6 along the multi-way bearing tube 1 to the surface of the uppermost reaction tank to reduce the usage amount of the fluid sample.

[0089] Step 4: Cover the multifunctional upper cover 5, turn on the power supply 507, turn on the driving pump 506, and inject a plurality of fluid samples into different pipelines of the multi-way bearing tube 1 through the sample inlet 505 respectively, so as to drive the imaging chip 4 and the reaction tank to rotate by means of the fluid sample.

[0090] Step 5: After the fluid sample treatment is completed, open the multifunctional upper cover 5, remove the variable-volume cavity 6, inject high-purity gas along the multi-way bearing tube 1, and perform high-efficiency drying treatment by using rotational centrifugal force.

[0091] Based on the above device for batch surface treatment and zonal functionalization of an imaging chip 4 using a fluid sample, steps 1 to 5 of the method may specifically be:

[0092] Step 1: Sequentially place the imaging chip 4 into the lower chip slot 301 of the lower reaction tank 3, and place and clamp and fix the imaging chip 4 close to the narrow side of the chip slot.

[0093] In a specific implementation, sequentially place the imaging chip 4 into the lower chip slot 301 of the lower reaction tank 3. When loading, pay attention to placing the imaging chip 4 close to the narrow side of the chip slot, ensure that the imaging chip 4 is vertical, so that the fluid flows down by gravity at the fastest speed to avoid stagnation, and at the same time facilitate the buckling of the upper reaction tank 2.

[0094] Step 2: Snap the imaging chip 4 into the chip sealing plate 7 one by one along the chip sealing slot 701, and gently twist and buckle the upper buckle 203 of the upper reaction tank 2 and the lower buckle 304 of the lower reaction tank 3. Pass the multi-way bearing tube 1 from bottom to top through the lower bearing hole 303 of the lower reaction tank 3 and the upper bearing hole 202 of the upper reaction tank 2. Repeat the above operations as needed for the installation of the remaining reaction tanks, and insert a reaction tank sealing plate 8 between two reaction tanks to separate the reaction tanks.

[0095] In a specific implementation, the chip sealing plate 7 is snapped into the imaging chip 4 one by one (if single-chip zoning is required). Then, the upper snap 203 on the upper reaction tank 2 is snapped onto the lower snap 304 on the lower reaction tank 3. After gently twisting and confirming the snap, the multi-channel bearing tube 1 is inserted from bottom to top through the lower bearing hole 303 in the lower reaction tank 3 and the upper bearing hole 202 in the upper reaction tank 2. Repeat the above operations for the installation of the upper reaction tank, and a reaction tank sealing plate 8 is inserted between the two reaction tanks to separate different reaction tanks.

[0096] Step 3: Place the assembled reaction tank into the variable-volume cavity 6, and slowly rotate the sealing piston 603 along the multi-channel bearing tube 1 to the surface of the uppermost reaction tank to reduce the amount of fluid sample used.

[0097] Step 4: Cover the multi-functional upper cover 5, turn on the power supply 507, and turn on the drive pump 506. Multiple fluid samples can be injected into different pipelines in the multi-channel bearing tube 1 through the sample inlet 505 respectively to achieve the processing of different fluid samples in different reaction tanks. When the sample is injected, the chip rotates clockwise. By changing the direction of the drive pump 506 for sample suction, the chip rotates counterclockwise. The chip can drive the reaction tank to rotate, playing a role in stirring and mixing, and can accelerate the surface treatment and functionalization rate.

[0098] Step 5: After the fluid sample is processed, open the multi-functional upper cover 5 and remove the variable-volume cavity 6. Inject high-purity gas (such as nitrogen) along the multi-channel bearing tube 1, and perform efficient drying treatment using rotational centrifugal force. When injecting the fluid, adjust the power of the drive pump 506 in a timely manner to control the flow rate to avoid the rotation speed of the reaction tank being greater than 100 RPM.

[0099] After considering the specification and the content disclosed herein, those skilled in the art will readily conceive of other embodiments of the present application. The present application is intended to cover any variations, uses, or adaptations of the present application, which follow the general principles of the present application and include the common general knowledge or conventional technical means in the technical field not disclosed in the present application.

[0100] It should be understood that the present application is not limited to the exact structure described above and shown in the drawings, and various modifications and changes can be made without departing from its scope.

Claims

1. An apparatus for batch surface treatment and zonal functionalization of an imaging chip using a fluid sample, characterized in that, It includes multiple bearing tubes, a multi-functional upper cover, a variable-volume cavity, a chip sealing plate, a reaction tank sealing plate, and several reaction tanks. The multiple bearing tubes are used to connect several of the reaction tanks in series and transfer and distribute fluid samples. The fluid samples are fluids that can be used for imaging chip processing and functionalization, including chemical reagents, biological samples, and high-purity gases. The reaction tanks are used to batch-load imaging chips and provide rotational power for the imaging chips through their turbine structures. The imaging chips are glass slides, ITO slides, gold slides, and other chips used for optical imaging. The multiple bearing tubes include an exhaust pipe and a chassis. The body of the exhaust pipe is provided with several circumferential exhaust holes. The number of the circumferential exhaust holes is the same as the number of the reaction tanks, and the circumferential exhaust holes are in one-to-one correspondence and communication with the reaction tanks. An upper chip slot and a lower chip slot are arranged in a single reaction tank, and are distributed in a turbine dynamics around the exhaust pipe, thus forming the turbine structure. The multi-functional upper cover is arranged above the variable-volume cavity and is used to realize the automatic sample injection and extraction of the fluid sample. The variable-volume cavity is used to load the reaction tanks, and its volume adapts to different numbers of reaction tanks to reduce the usage amount of the fluid sample. The chip sealing plate is arranged in the reaction tank and is used to realize the self-zoning of the batch imaging chips and achieve surface treatment or functionalization in different regions. The reaction tank sealing plate is arranged between every two reaction tanks and is used to seal the adjacent reaction tanks and cooperate with the multiple bearing tubes to realize the chip zoning functionalization of different reaction tanks.

2. The device according to claim 1, characterized in that The exhaust pipe is arranged on the chassis through a smooth bearing. A partitioned sample injection port is arranged at the upper end of the exhaust pipe, and several pipelines are arranged on the partitioned sample injection port to respectively communicate with the circumferential exhaust holes.

3. The device according to claim 1, characterized in that, The reaction tank includes an upper reaction tank and a lower reaction tank. The upper reaction tank includes an upper chip slot, an upper bearing hole, an upper buckle, and an upper smooth bearing. The lower reaction tank includes a lower chip slot, a water drainage hole, a lower bearing hole, and a lower buckle. The upper reaction tank and the lower reaction tank are connected in series through the upper bearing hole and the lower bearing hole by using the multiple bearing tubes. The upper chip slot and the lower chip slot are symmetric up and down. The upper smooth bearing is arranged at the top of the upper reaction tank and is used to reduce the friction caused by the rotation of the reaction tank. The upper buckle and the lower buckle cooperate with each other to make the upper reaction tank and the lower reaction tank fit tightly. The water drainage hole is arranged at the bottom of the lower reaction tank and is used to prevent the residual of the fluid sample.

4. The device according to claim 3, characterized in that, The planar shapes of the upper chip slot and the lower chip slot are trapezoids, and the outer side length is 1 / 2 - 2 / 3 of the inner side length.

5. The device according to claim 1, characterized in that, The multi-functional upper cover includes a timer, multi-channel pipelines, a partition board, a cover board, a sample injection port, a driving pump, and a power supply. The driving pump and the multi-channel pipelines are used to realize the sample injection, extraction, and switching of multiple fluid samples. The timer is used to record the processing time. The partition board separates the driving pump and the power supply for waterproofing the power supply. The cover board is used to close the multi-functional upper cover. The sample injection port is arranged on the cover board to connect the multi-channel pipelines and the partitioned sample injection port on the multiple bearing tubes.

6. The device according to claim 1, characterized in that, A sealed piston is arranged inside the variable-volume cavity, and the height of the sealed piston can be adjusted according to the number of reaction cells to change the cavity volume, so as to reduce the usage amount of the fluid sample. A groove slope is arranged at the bottom of the variable-volume cavity to prevent the generation of fluid dead volume from affecting the treatment effect. A sample outlet is arranged at the bottom of the side wall to discharge the fluid sample or communicate with the injection port to realize circular flow.

7. The device according to claim 1, characterized in that The chip sealing plate includes a chip sealing card slot and a bearing tube sealing sleeve. The chip sealing card slot is used to seal the chip, and the bearing tube sealing sleeve is used to seal the redundant surrounding exhaust holes.

8. A method for batch surface treatment and zonal functionalization of an imaging chip using a fluid sample, characterized in that, When applied to the device according to any one of claims 1-7, it includes the following steps: Step 1: Sequentially place the imaging chip into the reaction cells and clamp and fix it. Step 2: Snap the chip sealing plate according to the zoning requirements, pass the multi-channel bearing tube through several reaction cells from bottom to top, and insert a reaction cell sealing plate between every two adjacent reaction cells. Step 3: Place the assembled reaction cells and multi-channel bearing tube into the variable-volume cavity, and slowly rotate the sealed piston in the variable-volume cavity along the multi-channel bearing tube to the surface of the uppermost reaction cell. Step 4: Cover the multi-functional upper cover, turn on the power supply, turn on the drive pump, and inject multiple fluid samples into different pipelines of the multi-channel bearing tube through the injection port respectively, so as to drive the imaging chip and reaction cells to rotate through the fluid samples. Step 5: After the fluid sample treatment is completed, open the multi-functional upper cover, remove the variable-volume cavity, inject high-purity gas along the multi-channel bearing tube, and perform high-efficiency drying treatment by using rotational centrifugal force.

9. The method according to claim 8, wherein Steps 1 to 5 are specifically as follows: Step 1: Sequentially place the imaging chip into the lower chip card slot of the lower reaction cell, and place the imaging chip close to the narrow side of the chip card slot and clamp and fix it. Step 2: Snap the imaging chip into the chip sealing plate along the chip sealing card slot one by one, twist and fasten the upper buckle of the upper reaction cell and the lower buckle of the lower reaction cell, pass the multi-channel bearing tube through the lower bearing hole of the lower reaction cell and the upper bearing hole of the upper reaction cell from bottom to top, and repeat the above operations as needed for the installation of the remaining reaction cells. Insert a reaction cell sealing plate between every two reaction cells to separate the reaction cells. Step 3: Place the assembled reaction cells into the variable-volume cavity, and slowly rotate the sealed piston along the multi-channel bearing tube to the surface of the uppermost reaction cell. Step 4: Cover the multi-functional upper cover, turn on the power supply, turn on the drive pump, and inject multiple fluid samples into different pipelines of the multi-channel bearing tube through the injection port respectively to realize the treatment of different fluid samples in different reaction cells. When the sample is injected, the imaging chip rotates clockwise, and when the driving pump direction is changed and the sample is sucked, the chip rotates counterclockwise, and the imaging chip drives the reaction cells to rotate. Step 5: After the fluid sample treatment is completed, open the multi-functional upper cover, remove the variable-volume cavity, inject high-purity gas along the multi-channel bearing tube, and perform drying treatment by using rotational centrifugal force.

Images