A biological reagent processing system and method

Abstract

The present invention relates to the field of biological reagent technology, and more specifically, to a biological reagent processing system and method. A biological reagent processing system includes a box body, on which two slide grooves are symmetrically provided, and side frames are inserted in both slide grooves. A cylinder is fixedly connected between the two side frames, and a rubber filter plate is installed on the cylinder, and the rubber filter plate can be bent. A method for processing biological reagents in a biological reagent processing system, the method includes the following steps: a: injecting the biological reagent into the box body, driving the rubber filter plate to penetrate into a position below the liquid surface; b: after a layer of reaction film is precipitated on the liquid surface of the biological reagent, controlling the rubber filter plate to slide upward to separate the reaction film from the liquid surface; c: dripping the detection reagent on the reaction film; d: controlling the pressure roller to slide back and forth on the reaction film for rolling; e: determining the quality of the biological reagent by observing the reaction situation. The beneficial effect is that the dose of the detection reagent in contact with the biological agent reaction can be controlled.

Description

Technical Field

[0001] The present invention relates to the field of biological reagent technology, and more specifically to a biological reagent processing system and method. Background Art

[0002] There are many ways to use biological detection reagents. The most common method is to use a dropper to manually drip the biological detection reagent onto the surface of the biological tissue to be detected. However, this method makes it difficult to evenly cover the entire tissue surface with the reagent, and the amount of reagent cannot be accurately controlled, which easily causes reagent waste and has a certain impact on the test results. A biological reagent injection device with application number 201821177757.8, the utility model discloses a biological reagent injection device, including a test paper clamping table located above a rack workbench and moving back and forth along the Y-axis direction, and an injection mechanism located above the test paper clamping table and crossing the test paper clamping table and sliding back and forth along the X-axis direction. The injection mechanism includes an injection head that moves up and down relative to the test paper clamping table along the Z-axis direction and a micro pump located above the injection head and controlling the reagent delivery amount. A spray membrane is provided at the front end of the injection head, and a plurality of injection holes are provided on the injection membrane. The reagent is ejected from the injection membrane and atomized. The front end of the spray head of the utility model is provided with a spray film, which can evenly cover the biological reagent on the test paper to make a diagnostic test paper, and a micro pump can accurately and micro-control the delivery of the reagent to avoid waste. However, the device cannot control the dose of the test reagent and the biological agent, which will affect the test results of the biological reagent. Summary of the invention

[0003] In order to overcome the deficiencies of the prior art, the present invention provides a biological reagent processing system and method, which has the beneficial effect of being able to control the dose of the detection reagent and the biological agent reaction, so that the detection structure can be more accurate and the quality control of the biological reagent can be more accurate.

[0004] The technical solution adopted by the present invention to solve its technical problem is:

[0005] A biological reagent processing system comprises a box body, two slide grooves are symmetrically arranged on the box body, side frames are inserted in the two slide grooves, a cylinder is fixedly connected between the two side frames, a rubber filter plate is installed on the cylinder, and the rubber filter plate can be bent.

[0006] The lower end of the cylinder is fixedly connected to two vertical rods, and the two vertical rods are slidably connected to the hinge frame, and the hinge frame is hingedly connected to four hinge rods, and the four hinge rods are respectively hingedly connected to the four corners of the rubber filter plate.

[0007] A plurality of partitions are inserted on the rubber filter plate.

[0008] The plurality of partitions are all fixedly connected to a connecting frame, which is rotatably connected to two studs, and both ends of the connecting frame pass through a cylinder and a rubber filter plate and are respectively threadedly connected to the two studs.

[0009] A plurality of U-shaped frames are fixedly connected to the cylinder, a plurality of slots are arranged on the cylinder, the slots are communicated with the U-shaped frames, and the partitions are simultaneously inserted into the slots and the U-shaped frames that are communicated with each other.

[0010] A method for processing a biological reagent by a biological reagent processing system, the method comprising the following steps:

[0011] a: Inject the biological reagent into the box body, control the two side frames to slide downward, and drive the rubber filter plate to a position below the liquid surface;

[0012] b: After a layer of reaction film is precipitated on the liquid surface of the biological reagent, the two side frames are controlled to drive the cylinder and the rubber filter plate to slide upward, and the rubber filter plate lifts the reaction film upward until the reaction film is separated from the liquid surface;

[0013] c: The reagent adhering to the reaction membrane is filtered out through the filter holes on the rubber filter plate, and the detection reagent is dropped on the reaction membrane;

[0014] d: Press the roller onto the reaction membrane, control the roller to slide back and forth on the reaction membrane, and roll the reaction membrane and the detection reagent;

[0015] e: Determine the quality of biological reagents by observing and recording the reaction between the reaction membrane and the detection reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0017] Figure 1 A flowchart of a biological reagent processing method;

[0018] Figure 2 It is a schematic diagram of the internal structure of a biological reagent processing system;

[0019] Figure 3 It is a structural schematic diagram of a rubber filter plate;

[0020] Figure 4 It is a structural schematic diagram of an articulated frame;

[0021] Figure 5 is a schematic diagram of the structure of the partition;

[0022] Figure 6 It is a schematic diagram of the structure of a cylinder;

[0023] Figure 7 It is a structural schematic diagram of a U-shaped frame;

[0024] Figure 8 It is a structural schematic diagram of a pressure roller;

[0025] Fig. 9 It is a structural schematic diagram of the box body;

[0026] Fig.10 A schematic diagram of the structure of a biological reagent processing system. DETAILED DESCRIPTION

[0027] like Figure 2 and Figures 9 to 10 As shown:

[0028] A biological reagent processing system, characterized in that: it comprises a box body 101, two vertical slide grooves 102 are symmetrically arranged on the box body 101, side frames 103 are inserted into the two slide grooves 102, a first electric push rod is fixedly connected between the box body 101 and the two side frames 103, a cylinder 104 is fixedly connected between the two side frames 103, a horizontal rubber filter plate 105 is installed on the cylinder 104, the rubber filter plate 105 is fitted with the cylinder 104, and the rubber filter plate 105 is in a horizontal state under normal conditions, and secondly, the rubber filter plate 105 can be bent into an arc after being subjected to force;

[0029] The configured biological reagent to be detected is injected into the box body 101, and the two first electric push rods are controlled to start and retract to drive the two side frames 103 to slide downward. The side frames 103 drive the rubber filter plate 105 in a horizontal state to slide downward through the cylinder 104 and penetrate into the position below the liquid surface of the biological reagent. After a layer of reaction film is precipitated on the liquid surface of the biological reagent, the movable rods on the two first electric push rods are controlled to extend upward, and the two side frames 103 drive the cylinder 104 and the rubber filter plate 105 to slide upward. The rubber filter plate 105 lifts the reaction membrane upward until the reaction membrane is separated from the liquid surface. The biological reagent attached to the reaction membrane is filtered out through the filter holes on the rubber filter plate 105, so that only a layer of reaction membrane is left on the rubber filter plate 105. The detection reagent is dripped on the reaction membrane, and the quality of the biological reagent is determined by observing and recording the reaction between the reaction membrane and the detection reagent.

[0030] When the rubber filter plate 105 is in a horizontal state, the supported reaction membrane is also in a horizontal state, and the detection reagent dripped on the reaction membrane will remain on the reaction membrane for reaction, so it is suitable for observing the reaction situation to determine the quality of the biological reagent when the reaction membrane of the biological reagent and the detection reagent have a long contact reaction time; a sealing layer can be applied on the rubber filter plate 105, so as to protect the rubber filter plate 105 and prevent the reaction after the detection reagent and the biological reagent come into contact with each other and cause damage to the rubber filter plate 105;

[0031] When both sides of the rubber filter plate 105 are pulled downward at the liquid surface, so that the rubber filter plate 105 presents a convex arc shape, when the arc-shaped rubber filter plate 105 moves upward, the lifted reaction membrane is attached to the arc-shaped rubber filter plate 105 and also presents an arc shape. At this time, after the detection reagent is dropped from the top of the rubber filter plate 105 on the arc-shaped reaction membrane, under the action of gravity, the detection reagent will slide down from the middle of the reaction membrane to both sides, so that only a thin layer of the detection reagent is left on the reaction membrane to contact and react with the biological agent reaction membrane, and then the reaction of the arc-shaped reaction membrane and the detection reagent can be judged by observing the instantaneous contact reaction between the arc-shaped reaction membrane and the detection reagent. The quality of biological reagents can be judged; therefore, the system is suitable for detecting biological reagents of different components. It can be used in situations where a long reaction waiting period is required after the biological reagent and the detection reagent come into contact, and it can also be used in situations where the biological reagent and the detection reagent react instantly after contact and do not require a reaction waiting period. In this case, by changing the shape of the rubber filter plate 105, the dosage of the detection reagent in contact with the biological reagent reaction membrane can be controlled, so that the biological reagent and the detection reagent can achieve an instantaneous contact, thereby making the detection structure more accurate and making the quality control of the biological reagent more accurate.

[0032] like Figures 3 to 4 As shown:

[0033] The lower end of the cylinder 104 is welded to two vertical rods 109, and the two vertical rods 109 are slidably connected to the hinge frame 107, and the hinge frame 107 is hinged to four hinge rods 106, and the four hinge rods 106 are respectively hinged to the four corners of the rubber filter plate 105;

[0034] When the hinged frame 107 slides downward on the two vertical rods 109, the hinged frame 107 pulls the four corners of the rubber filter plate 105 downward through the four hinged rods 106. Since the middle of the rubber filter plate 105 is in contact with the cylinder 104, the cylinder 104 provides a certain support to the middle of the rubber filter plate 105. Therefore, when the two ends of the rubber filter plate 105 are subjected to downward pulling force, the middle of the rubber filter plate 105 remains stationary, and the two ends of the rubber filter plate 105 bend downward to form a convex arc shape. Therefore, the supported reaction membrane can also present an arc shape, and the detection reagent dripped on the arc-shaped reaction membrane can slide downward instantly and will not remain on the reaction membrane, thereby achieving the purpose of instant contact with the reaction membrane for reaction;

[0035] By controlling the downward movement distance of the articulated frame 107, the curvature of the rubber filter plate 105 can be changed, and the speed at which the detection reagent dripped on the arc-shaped reaction membrane slides downward can be controlled, thereby controlling the contact reaction time between the detection reagent and the biological reagent, thereby being suitable for detecting biological reagents with different reaction speeds produced by the detection reagent.

[0036] like Figure 5 As shown:

[0037] A plurality of partitions 203 are inserted on the rubber filter plate 105. When the rubber filter plate 105 is below the liquid surface, when the biological reagent that has not formed a reaction film in the initial stage is detected, the plurality of partitions 203 are controlled to slide downward for a distance but still maintain contact with the rubber filter plate 105. At this time, a plurality of square grooves are formed between the upper ends of the plurality of partitions 203 and the top surface of the rubber filter plate 105. The rubber filter plate 105 and the partitions 203 are controlled to move upward synchronously and leave the liquid surface, and the biological reagent will enter the plurality of square grooves. Since the rubber filter plate 105 is provided with filter holes, no biological reagent will remain on the rubber filter plate 105. At this time, detection reagents of different components can be added to the biological reagents in the plurality of square grooves respectively, and a comparative experiment can be conducted by observing the reaction between the biological reagent in each square groove and the detection reagent, so as to obtain more accurate detection data of the biological reagent.

[0038] Secondly, when the rubber filter plate 105 is in a horizontal state and holds up the reaction membrane, the plurality of partitions 203 are controlled to extend upward from the upper end of the rubber filter plate 105, and the plurality of partitions 203 extending upward cut off the reaction membrane, and at the same time divide the rubber filter plate 105 into a plurality of areas. At this time, a plurality of detection reagents of different components can be respectively dropped on the reaction membranes in different areas, so as to facilitate comparative experiments by observing the response of a plurality of reaction membranes, without the need to take out the reaction membrane and cut it into a plurality of pieces for detection;

[0039] Furthermore, since the rubber filter plate 105 is made of a deformable rubber material, when the two ends of the rubber filter plate 105 are bent and deformed downward, the rubber filter plate 105 will be stretched at the contact point with the partition 203, but the partition 203 will not affect the bending deformation process of the rubber filter plate 105; at the same time, when the rubber filter plate 105 is bent into an arc, multiple partitions 203 slide upward, which will also separate the arc-shaped rubber filter plate 105 into multiple areas, facilitating comparative experiments.

[0040] like Figures 5 to 7 As shown:

[0041] The plurality of partitions 203 are all connected to the connecting frame 204 by screws, and the connecting frame 204 is rotatably connected to two studs 205, and both ends of the connecting frame 204 pass through the cylinder 104 and the rubber filter plate 105 and are respectively threadedly connected to the two studs 205; when the two studs 205 are rotated, the two studs 205 drive the connecting frame 204 to move up and down, and then drive the plurality of partitions 203 to move up and down through the connecting frame 204, so that the plurality of partitions 203 can separate the rubber filter plate 105 or form square grooves with the rubber filter plate 105;

[0042] Secondly, when the rubber filter plate 105 is below the liquid surface, the two studs 205 are both above the liquid surface, thereby facilitating manual rotation of the two studs 205 without contaminating the biological reagent.

[0043] like Figure 7 As shown:

[0044] A plurality of U-shaped frames 202 are welded and connected to the cylinder 104, and a plurality of slots 201 are provided on the cylinder 104, and the slots 201 correspond to and are connected with the U-shaped frames 202, and the partitions 203 are inserted into the slots 201 and the U-shaped frames 202 that are connected with each other at the same time; the plurality of partitions 203 divide the cylinder 104 into multiple ends, and the plurality of U-shaped frames 202 enable the multiple sections of the cylinder 104 to remain in a whole state, so that the partitions 203 can separate the intervals at the upper end of the rubber filter plate 105, and will not affect the support of the cylinder 104 for the middle part of the rubber filter plate 105, so that the middle part of the rubber filter plate 105 is immovable and then bent into an arc shape.

[0045] like Figure 4 As shown:

[0046] A slide 108 is welded to each end of the articulated frame 107, and both slides 108 pass through the cylinder 104 and are slidably connected to the two side frames 103. A second electric push rod is fixedly connected between the side frames 103 and the slide 108; the two second electric push rods are respectively located inside the two side frames 103. When the two second electric push rods are started, the two slides 108 are driven to move up and down, and then the articulated frame 107 is driven to move up and down. The articulated frame 107 drives the two ends of the rubber filter plate 105 to bend under force or restore the rubber filter plate 105 to a horizontal state through four articulated rods 106.

[0047] like Figure 8 As shown:

[0048] The cylinder 104 is symmetrically fixed with two fixing frames 301 by screws, and the two fixing frames 301 are matched with roller frames 303, and the roller frames 303 are rotatably connected with a pressure roller 304; when the detection reagent is dropped on the reaction membrane, the detection reagent on the reaction membrane is in the shape of a water droplet, and cannot fully contact and react with the reaction membrane well. After the pressure roller 304 is controlled to fit on the reaction membrane, the pressure roller 304 is made to slide back and forth between the two fixing frames 301, and under the action of the friction force of contact with the reaction membrane, the pressure roller 304 is driven to rotate, and the rotating pressure roller 304 can roll and expand the detection reagent, so that the detection reagent and the reaction membrane can fully contact and react, thereby avoiding the detection reagent gathering at one point and failing to fully play the detection role.

[0049] like Figure 8 As shown:

[0050] Two pillars 305 are welded and connected to the roller frame 303, and the two pillars 305 are inserted into the cross beam 302. Two springs are fixedly connected between the pillars 305 and the cross beam 302, and the two springs are respectively sleeved on the two pillars 305. The cross beam 302 is slidably connected to the two fixed frames 301; a lead screw is threadedly connected between the cross beam 302 and the fixed frame 301, and a stepper motor is fixedly connected to the fixed frame 301, and the output shaft of the stepper motor is connected to the lead screw through a coupling;

[0051] The stepper motor starts to drive the lead screw to rotate, and the lead screw drives the cross beam 302, the roller frame 303 and the pressure roller 304 to slide back and forth between the two fixed frames 301, thereby driving the pressure roller 304 to roll back and forth on the reaction membrane, so that the detection reagent is fully immersed in the reaction membrane, and a sufficient contact reaction is carried out, so that the detection reagent can play a maximum effect;

[0052] The two springs enable the pressure roller 304 to be elastically pressed against the reaction membrane. Therefore, when the rubber filter plate 105 is in an arc-shaped curved shape, the elastic force of the spring enables the pressure roller 304 to always adhere to the reaction membranes of different curvatures, thereby rolling the detection reagent on the arc-shaped reaction membrane, so that the detection reagent remaining on the arc-shaped surface can fully contact and react with the reaction membrane.

[0053] like Figure 8 As shown:

[0054] The crossbeam 302 is threadedly connected with locking screws corresponding to the column 305;

[0055] Before injecting the biological reagent into the box body 101, pull up the two pillars 305, tighten the locking screws and press them tightly against the pillars 305, and fix the position of the pressure roller 304. At this time, there is a certain distance between the pressure roller 304 and the rubber filter plate 105. When the rubber filter plate 105 moves below the liquid surface, the pressure roller 304 is above the liquid surface, so that the pressure roller 304 will not contact the biological reagent. When the rubber filter plate 105 holds up the reaction membrane and drips the detection reagent, the locking screws are loosened, and the pressure roller 304 is released and elastically pressed on the reaction membrane to roll the detection reagent.

[0056] A method for processing a biological reagent by a biological reagent processing system, the method comprising the following steps:

[0057] a: inject the biological reagent into the box body 101, control the two side frames 103 to slide downward, and drive the rubber filter plate 105 to a position below the liquid surface;

[0058] b: After a layer of reaction film is precipitated on the liquid surface of the biological reagent, the two side frames 103 are controlled to drive the cylinder 104 and the rubber filter plate 105 to slide upward, and the rubber filter plate 105 lifts the reaction film upward until the reaction film is separated from the liquid surface;

[0059] c: The reagent adhered to the reaction membrane is filtered out through the filter holes on the rubber filter plate 105, and the detection reagent is dropped on the reaction membrane;

[0060] d: Press the pressing roller 304 onto the reaction membrane, control the pressing roller 304 to slide back and forth on the reaction membrane, and roll the reaction membrane and the detection reagent;

[0061] e: Determine the quality of biological reagents by observing and recording the reaction between the reaction membrane and the detection reagent.

Claims

1. A biological reagent processing method, characterized in that: The method is implemented by a biological reagent processing system, the system comprising a box body (101), two slide grooves (102) are symmetrically arranged on the box body (101), side frames (103) are inserted into the two slide grooves (102), a cylinder (104) is fixedly connected between the two side frames (103), a rubber filter plate (105) is installed on the cylinder (104), and the rubber filter plate (105) can be bent; The lower end of the cylinder (104) is fixedly connected to two vertical rods (109), and the two vertical rods (109) are slidably connected to a hinged frame (107). The hinged frame (107) is hingedly connected to four hinged rods (106), and the four hinged rods (106) are respectively hingedly connected to the four corners of the rubber filter plate (105); A plurality of partitions (203) are inserted on the rubber filter plate (105); The plurality of partitions (203) are all fixedly connected to a connecting frame (204), the connecting frame (204) is rotatably connected to two studs (205), and both ends of the connecting frame (204) pass through the cylinder (104) and the rubber filter plate (105) and are respectively threadedly connected to the two studs (205); A plurality of U-shaped frames (202) are fixedly connected to the cylinder (104), a plurality of slots (201) are provided on the cylinder (104), the slots (201) are connected to the U-shaped frames (202), and the partitions (203) are simultaneously inserted into the slots (201) and the U-shaped frames (202) that are connected to each other; The two ends of the articulated frame (107) are respectively fixedly connected to a slide frame (108), and the two slide frames (108) both pass through the cylinder (104) and are slidably connected to the two side frames (103); The cylinder (104) is symmetrically fixedly connected to two fixing frames (301), the two fixing frames (301) are cooperatively connected to roller frames (303), and the roller frames (303) are rotatably connected to a pressure roller (304); The roller frame (303) is fixedly connected to two poles (305), the two poles (305) are both inserted into the crossbeam (302), a spring is fixedly connected between the poles (305) and the crossbeam (302), and the crossbeam (302) is slidably connected to the two fixed frames (301); The method comprises the following steps: a: injecting the biological reagent into the box body (101), controlling the two side frames (103) to slide downward, and driving the rubber filter plate (105) to penetrate into a position below the liquid surface; b: After a layer of reaction film is precipitated on the liquid surface of the biological reagent, the two side frames (103) are controlled to drive the cylinder (104) and the rubber filter plate (105) to slide upward, and the rubber filter plate (105) lifts the reaction film upward until the reaction film is separated from the liquid surface; c: The biological reagent attached to the reaction membrane is filtered out through the filter holes on the rubber filter plate (105), and the detection reagent is dropped on the reaction membrane; d: pressing the pressing roller (304) onto the reaction membrane, controlling the pressing roller (304) to slide back and forth on the reaction membrane, and rolling the reaction membrane and the detection reagent; e: Determine the quality of biological reagents by observing and recording the reaction between the reaction membrane and the detection reagent.

2. A biological reagent processing method according to claim 1, characterized in that: The crossbeam (302) is threadedly connected with a locking screw corresponding to the column rod (305).

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