Tip for digital PCR microdroplet generation

Abstract

The present application provides a pipette tip for digital PCR microdroplet generation, which includes a pipette tip body. The pipette tip body has a hollow tubular structure; a plurality of reinforcing ribs are annularly and arrayedly distributed on the outer peripheral surface of the pipette tip body, and each reinforcing rib extends from one end of the pipette tip body towards the other end of the pipette tip body. In the present application, a plurality of reinforcing ribs are annularly and arrayedly arranged on the outer peripheral surface of the pipette tip body, and the length direction of each reinforcing rib is consistent with the length direction of the pipette tip body, so that the rigidity of the pipette tip can be greatly improved. The pipette tip will not undergo soft deformation due to high-frequency vibration during the digital PCR detection experiment, which helps to improve the quality of microdroplet generation.

Description

Technical Field

[0001] This application belongs to the technical field of micro-droplet generation, and more specifically, relates to a pipette tip for digital PCR micro-droplet generation. Background Art

[0002] Digital PCR (Polymerase chain reaction) detection technology is a molecular biology technology used to amplify specific DNA (DeoxyriboNucleic Acid) fragments. It can be regarded as a special DNA replication outside the organism, which can greatly increase trace amounts of DNA. The micro-droplet generation technology is an important intermediate link in the digital PCR detection technology, and the quality of micro-droplet generation will directly affect the accuracy of the experiment.

[0003] However, current ordinary pipette tips are prone to soft deformation due to high-frequency vibration in digital PCR detection experiments, resulting in problems such as insufficient rigidity and pipette tip swaying, thus affecting the quality of micro-droplet generation. Summary of the Invention

[0004] The purpose of the embodiments of this application is to provide a pipette tip for digital PCR micro-droplet generation to solve the problems in the related art: ordinary pipette tips are prone to soft deformation due to high-frequency vibration in digital PCR detection experiments, resulting in problems such as insufficient rigidity and pipette tip swaying, thus affecting the quality of micro-droplet generation.

[0005] To achieve the above purpose, the technical solution adopted in the embodiments of this application is:

[0006] Provide a pipette tip for digital PCR micro-droplet generation, including:

[0007] A pipette tip body, the pipette tip body having a hollow tubular structure;

[0008] A plurality of reinforcing ribs, the plurality of reinforcing ribs being annularly and arrayedly distributed on the outer peripheral surface of the pipette tip body, and each of the reinforcing ribs extending from one end of the pipette tip body towards the other end of the pipette tip body.

[0009] With this structure, in this application, a plurality of reinforcing ribs are annularly and arrayedly arranged on the outer peripheral surface of the pipette tip body, and the length direction of each reinforcing rib is consistent with the length direction of the pipette tip body, thereby greatly improving the rigidity of the pipette tip. This pipette tip will not undergo soft deformation due to high-frequency vibration in digital PCR detection experiments, and thus helps to improve the quality of micro-droplet generation.

[0010] In one embodiment, one end of the tip body is a first liquid inlet, the other end of the tip body is a first liquid outlet, and the diameter of the tip body gradually decreases from the direction of the first liquid inlet towards the first liquid outlet.

[0011] With this structure, since the diameter of the first liquid inlet is larger than that of the first liquid outlet, the tip can stably generate PCR microdroplets, and can meet the high-throughput requirements as needed. The generated microdroplets are characterized by high efficiency and high quality.

[0012] In one embodiment, the tip for digital PCR microdroplet generation further includes a connection guide plate installed at one end of the tip body away from the first liquid outlet, and a through hole communicating with the first liquid inlet is provided on the connection guide plate.

[0013] With this structure, it is convenient to connect and fix the tip to external components through the connection guide plate, thus facilitating the fixation of the tip.

[0014] In one embodiment, the diameter of the through hole gradually decreases from the direction of the first liquid inlet towards the first liquid outlet.

[0015] With this structure, the diameters of the through hole, the first liquid inlet, and the first liquid outlet decrease step by step, which can ensure the stable generation of high-throughput microdroplets.

[0016] In another embodiment, the liquid outlet end of the tip body is elliptical.

[0017] With this structure, compared with the circular first liquid outlet, the shear force received by each point of the droplet at the elliptical first liquid outlet is more uniform, which is more conducive to the droplet being cut off at the first liquid outlet. Thus, it effectively improves the problem that it is difficult to cut off the PCR microdroplets when using an ordinary tip, resulting in uneven microdroplet sizes, and significantly improves the efficiency of microdroplet generation.

[0018] In yet another embodiment, the tip body has a cylindrical configuration, the top of the tip body is a second liquid inlet, the bottom of the tip body is closed, and a second liquid outlet is provided on the tip body.

[0019] With this structure, the liquid entering from the second liquid inlet can be discharged from the second liquid outlet. The droplet at the second liquid outlet is cut off under the action of shear force. On the premise of ensuring strength and rigidity, PCR microdroplets can be stably generated, and can meet the high-throughput requirements as needed. The generated microdroplets are characterized by high efficiency and high quality.

[0020] In yet another embodiment, the second liquid outlet is provided on the outer side wall of the tip body;

[0021] Alternatively, the second liquid outlet is provided at the bottom of the tip body.

[0022] With this structure, the vibration direction of the gun head can be adjusted by the position of the second liquid outlet, thereby stably generating micro-droplets.

[0023] In yet another embodiment, the gun head body includes a first section in a cylindrical configuration and a second section connected to the first section. Both ends of the first section are open, and the top of the first section is a third liquid inlet; the second section is in a hemispherical configuration and protrudes away from the first section, and a third liquid outlet is provided on the gun head body.

[0024] With this structure, by setting the bottom of the gun head body to be semi-circular, the characteristics of high throughput and high quality of micro-droplet generation can be ensured.

[0025] In yet another embodiment, the third liquid outlet is provided on the outer side wall of the first section;

[0026] Alternatively, the third liquid outlet is provided on the second section.

[0027] With this structure, the vibration direction of the gun head can be adjusted by the position of the third liquid outlet, thereby stably generating micro-droplets.

[0028] In still another embodiment, the gun head body is in an inverted cone configuration, the top of the gun head body is a fourth liquid inlet, and a fourth liquid outlet is provided on the outer side wall of the gun head body.

[0029] With this structure, the liquid entering from the fourth liquid inlet can be discharged from the fourth liquid outlet, thereby stably generating micro-droplets. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for use in the embodiments or exemplary technical descriptions. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0031] Figure 1 It is a three-dimensional structure schematic diagram of the gun head provided in the first embodiment of the present application;

[0032] Figure 2 For Figure 1 the cross-sectional schematic diagram of;

[0033] Figure 3 It is a cross-sectional schematic diagram of a gun head provided in the third embodiment of the present application Figure 1 ;

[0034] Figure 4 It is a cross-sectional schematic diagram of a gun head provided in the third embodiment of the present applicationFigure 2 ;

[0035] Figure 5 Cross-sectional schematic of another type of tip provided in Embodiment 3 of the present application Figure 1 ;

[0036] Figure 6 Cross-sectional schematic of another type of tip provided in Embodiment 3 of the present application Figure 2 ;

[0037] Figure 7 Cross-sectional schematic of a tip provided in Embodiment 4 of the present application Figure 1 ;

[0038] Figure 8 Cross-sectional schematic of a tip provided in Embodiment 4 of the present application Figure 2 ;

[0039] Figure 9 Cross-sectional schematic of another type of tip provided in Embodiment 4 of the present application Figure 1 ;

[0040] Figure 10 Cross-sectional schematic of another type of tip provided in Embodiment 4 of the present application Figure 2 ;

[0041] Figure 11 Cross-sectional schematic of the tip provided in Embodiment 5 of the present application Figure 1 ;

[0042] Figure 12 Cross-sectional schematic of the tip provided in Embodiment 5 of the present application Figure 2 ;

[0043] Figure 13 Generation diagram of microdroplets obtained by applying a common tip to a PCR experiment;

[0044] Figure 14 Generation diagram of microdroplets obtained by applying the tip provided in any one of Embodiments 1 to 5 of the present application to a PCR experiment.

[0045] Among them, the main reference signs in each figure are as follows:

[0046] 1. Tip body; 11. First liquid inlet; 12. First liquid outlet; 13. Second liquid inlet; 14. Second liquid outlet; 15. First section; 150. Third liquid inlet; 16. Second section; 17. Third liquid outlet; 18. Fourth liquid inlet; 19. Fourth liquid outlet;

[0047] 2. Reinforcing rib position; 21. First cut; 22. Second cut;

[0048] 3. Connecting guide plate; 30. Through hole. Detailed implementation manners

[0049] In order to make the technical problems, technical solutions and beneficial effects to be solved by this application more clearly understood, the following further details this application in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain this application and are not used to limit this application.

[0050] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0051] In addition, the terms "first", "second", "third", "fourth" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first", "second", "third", "fourth" may explicitly or implicitly include one or more of such features. In the description of this application, "a plurality of" means two or more, unless otherwise specifically defined. "Several" means one or more, unless otherwise specifically defined.

[0052] In the description of this application, it should be understood that the orientation or positional relationship indicated by the terms "center", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be understood as a limitation to this application.

[0053] In the description of this application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0054] Reference to "one embodiment" or "an embodiment" throughout the specification means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present application. Thus, the phrases "in one embodiment" or "in some embodiments" appearing throughout the specification do not necessarily refer to the same embodiment. In addition, in one or more embodiments, the particular features, structures, or characteristics may be combined in any suitable manner.

[0055] Embodiment 1:

[0056] Please refer to Figure 1 and Figure 2 to describe the pipette tip for digital PCR microdroplet generation provided in Embodiment 1 of the present application. The pipette tip for digital PCR microdroplet generation includes a pipette tip body 1 having a hollow tubular structure and a plurality of reinforcing ribs 2 distributed in an annular array on the outer peripheral surface of the pipette tip body 1. Wherein, both ends of the pipette tip body 1 are open ends, and the liquid entering from the liquid inlet end of the pipette tip body 1 can be discharged from the liquid outlet end of the pipette tip body 1, and microdroplets are formed during the vibration of the pipette tip. Each reinforcing rib 2 extends from one end of the pipette tip body 1 towards the other end of the pipette tip body 1, that is, the length direction of each reinforcing rib 2 is consistent with the length direction of the pipette tip body 1, thereby greatly improving the rigidity of the pipette tip. The pipette tip will not be soft and deformed due to high-frequency vibration during the digital PCR detection experiment, which helps to improve the quality of microdroplet generation.

[0057] In one embodiment, the plurality of reinforcing ribs 2 and the pipette tip body 1 can be integrally formed, which can ensure the mechanical strength of the pipette tip, is also convenient for processing and manufacturing, and has high efficiency. Among them, the material of the pipette tip can be selected from fully transparent medical plastics or thin-walled stainless steel, which has certain hydrophobic and oleophobic properties.

[0058] In one embodiment, please refer to Figure 2 As a specific implementation manner of the pipette tip for digital PCR microdroplet generation provided in Embodiment 1 of the present application, one end of the pipette tip body 1 is a first liquid inlet 11, the other end of the pipette tip body 1 is a first liquid outlet 12, and the diameter of the pipette tip body 1 gradually decreases from the first liquid inlet 11 towards the first liquid outlet 12. With this structure, the liquid entering from the first liquid inlet 11 can be discharged from the first liquid outlet 12. Since the diameter of the first liquid inlet 11 is larger than the diameter of the first liquid outlet 12, the pipette tip can stably generate PCR microdroplets and can meet the high-throughput requirements according to the requirements. The generated microdroplets have the characteristics of high efficiency and high quality.

[0059] In one embodiment, the configurations of the first liquid inlet 11 and the first liquid outlet 12 can both be circular, and the first liquid inlet 11 and the second liquid inlet 13 are coaxially arranged, thereby ensuring the smoothness of liquid flow.

[0060] In one embodiment, when the first liquid outlet 12 is circular, the diameter range of the first liquid outlet 12 is 10 μm - 1000 μm. With this structure, the micro-droplets discharged from the first liquid outlet 12 have good uniformity and high efficiency, and can meet the requirements of high throughput.

[0061] In one embodiment, please refer to Figure 1 and Figure 2 , as a specific implementation of the tip for digital PCR micro-droplet generation provided in Embodiment 1 of the present application, the tip for digital PCR micro-droplet generation further includes a connection guide plate 3 installed at one end of the tip body 1 away from the first liquid outlet 12. A through hole 30 communicating with the first liquid inlet 11 is provided on the connection guide plate 3. With this structure, it is convenient to connect and fix the tip to an external component through the connection guide plate 3, thereby facilitating the fixation of the tip. The through hole 30 communicates with the first liquid inlet 11, facilitating the liquid supplied by the liquid supply device to smoothly enter the tip and realizing the generation of micro-droplets.

[0062] In one embodiment, the through hole 30 and the first liquid inlet 11 are coaxially arranged, so as to ensure the smoothness of liquid flow.

[0063] In one embodiment, the connection guide plate 3, the tip body 1 and the plurality of reinforcing ribs 2 can be integrally formed, so as to ensure the mechanical strength of the tip, and is also convenient for processing and manufacturing, with high efficiency.

[0064] In one embodiment, please refer to Figure 2 , as a specific implementation of the tip for digital PCR micro-droplet generation provided in Embodiment 1 of the present application, the diameter of the through hole 30 gradually decreases from the first liquid inlet 11 towards the first liquid outlet 12. Specifically, the diameter of the open end of the through hole 30 away from the first liquid inlet 11 is the largest, the diameter of the open end of the through hole 30 close to the first liquid inlet 11 is the smallest, and the diameter of the open end of the through hole 30 close to the first liquid inlet 11 is equal to the diameter of the first liquid inlet 11. With this structure, the diameters of the through hole 30, the first liquid inlet 11 and the first liquid outlet 12 decrease step by step, which can ensure the stable generation of high-throughput micro-droplets.

[0065] In one embodiment, please refer to Figure 1, each reinforcing rib 2 may include a first section 21 installed at one end of the outer peripheral surface of the gun head body 1 close to the connecting guide plate 3 and a second section 22 connected to the first section 21. One end of each first section 21 is connected to the connecting guide plate 3, the other end of each first section 21 is connected to one end of the corresponding second section 22, and the other end of each second section 22 extends towards the direction close to the first liquid outlet 12. Among them, the length of each first section 21 is less than the length of the corresponding second section 22, and the thickness of each first section 21 is greater than the thickness of the corresponding second section 22. Here, the above-mentioned length can be understood as the length in the axial direction of the gun head body 1, and the above-mentioned thickness can be understood as the thickness in the radial direction of the gun head body 1. With this structure, by setting each reinforcing rib 2 as the first section 21 and the second section 22, the strength of the gun head can be ensured to be consistent at each position, thereby improving the overall strength of the gun head and avoiding problems such as soft deformation and insufficient rigidity and swaying of the gun head during high-frequency vibration.

[0066] Embodiment Two:

[0067] Now, the gun head for digital PCR microdroplet generation provided in the second embodiment of the present application will be described. The gun head for digital PCR microdroplet generation provided in the second embodiment of the present application is an improvement based on the gun head for digital PCR microdroplet generation provided in the first embodiment. The difference between the gun head for digital PCR microdroplet generation provided in the second embodiment of the present application and the gun head for digital PCR microdroplet generation provided in the above-mentioned first embodiment is that: the liquid outlet end of the gun head body 1 is elliptical, that is, the first liquid outlet 12 is elliptical. With this structure, compared with the circular first liquid outlet 12, the shear force received by each point of the liquid droplets at the elliptical first liquid outlet 12 is more uniform, which is more conducive to the microdroplets being cut off at the first liquid outlet 12, thereby effectively improving the problem that it is difficult to cut off the PCR microdroplets when using ordinary gun heads, resulting in uneven microdroplet sizes, and significantly improving the efficiency of microdroplet generation.

[0068] The other structures of the gun head for digital PCR microdroplet generation provided in the second embodiment of the present application are the same as the corresponding structures of the gun head for digital PCR microdroplet generation provided in the above-mentioned first embodiment, and will not be elaborated here one by one.

[0069] Embodiment Three:

[0070] Please refer to Figure 3 and Figure 5, the pipette tip for digital PCR microdroplet generation provided in Embodiment III of the present application will be described. The pipette tip for digital PCR microdroplet generation provided in Embodiment III of the present application is an improvement based on the pipette tip for digital PCR microdroplet generation provided in Embodiment I. The difference between the pipette tip for digital PCR microdroplet generation provided in Embodiment III of the present application and the pipette tip for digital PCR microdroplet generation provided in the above Embodiment I is as follows: The pipette tip body 1 is in a cylindrical configuration. The top of the pipette tip body 1 is the second liquid inlet 13. The bottom of the pipette tip body 1 is closed, and a second liquid outlet 14 is provided on the pipette tip body 1. With this structure, the liquid entering from the second liquid inlet 13 can be discharged from the second liquid outlet 14. The droplets at the second liquid outlet 14 are sheared under the action of shear force. On the premise of ensuring strength and rigidity, PCR microdroplets can be stably generated, and the high-throughput requirements can be met according to the requirements. The generated microdroplets have the characteristics of high efficiency and high quality. The droplets generated by this pipette tip in digital PCR experiments are uniform and stable, which can effectively solve the problems of low microdroplet generation efficiency, uneven size, and easy generation of microdroplet fragments in the current PCR detection field.

[0071] In one embodiment, please refer to Figure 3 and Figure 5 , as a specific implementation manner of the pipette tip for digital PCR microdroplet generation provided in Embodiment III of the present application, the second liquid outlet 14 is provided on the outer side wall of the pipette tip body 1; or, the second liquid outlet 14 is provided at the bottom of the pipette tip body 1. With this structure, the vibration direction of the pipette tip can be adjusted through the position of the second liquid outlet 14, thereby stably generating microdroplets.

[0072] In one embodiment, please refer to Figure 4 and Figure 6 , the number of the second liquid outlets 14 can be multiple, and the multiple second liquid outlets 14 are symmetrically distributed with respect to the central axis of the pipette tip body 1. With this structure, the pipette tip can generate multiple microdroplets at one time, thereby meeting the high-throughput requirements and having high efficiency. Among them, the number of the second liquid outlets 14 can be two, four, etc., and there is no unique limitation here.

[0073] The other structures of the pipette tip for digital PCR microdroplet generation provided in Embodiment III of the present application are the same as the corresponding structures of the pipette tip for digital PCR microdroplet generation provided in the above Embodiment I, and will not be elaborated one by one here.

[0074] Embodiment IV:

[0075] Please refer to Figure 7 and Figure 9, the pipette tip for digital PCR microdroplet generation provided in the fourth embodiment of the present application will now be described. The pipette tip for digital PCR microdroplet generation provided in the fourth embodiment of the present application is an improvement based on the pipette tip for digital PCR microdroplet generation provided in the first embodiment. The difference between the pipette tip for digital PCR microdroplet generation provided in the fourth embodiment of the present application and the pipette tip for digital PCR microdroplet generation provided in the above-mentioned first embodiment is that: the pipette tip body 1 includes a first section 15 in a cylindrical configuration and a second section 16 connected to the first section 15. Both ends of the first section 15 are open, and the top of the first section 15 is a third liquid inlet 150; the second section 16 is in a hemispherical configuration and protrudes away from the first section 15, and a third liquid outlet 17 is provided on the pipette tip body 1. With this structure, by setting the bottom of the pipette tip body 1 to be semi-circular, the characteristics of high throughput and high quality of microdroplet generation can be ensured.

[0076] In one embodiment, please refer to Figure 7 and Figure 9 , as a specific implementation manner of the pipette tip for digital PCR microdroplet generation provided in the fourth embodiment of the present application, the third liquid outlet 17 is provided on the outer side wall of the first section 15; alternatively, the third liquid outlet 17 is provided on the second section 16. With this structure, the vibration direction of the pipette tip can be adjusted through the position of the third liquid outlet 17, thereby stably generating microdroplets.

[0077] In one embodiment, please refer to Figure 8 and Figure 10 , the number of the third liquid outlets 17 can be multiple, and the multiple third liquid outlets 17 are symmetrically distributed with respect to the central axis of the pipette tip body 1. With this structure, the pipette tip can generate multiple microdroplets at one time, thereby meeting the requirements of high throughput and having high efficiency. Among them, the number of the third liquid outlets 17 can be two, four, etc., and is not uniquely limited herein.

[0078] Other structures of the pipette tip for digital PCR microdroplet generation provided in the fourth embodiment of the present application are the same as the corresponding structures of the pipette tip for digital PCR microdroplet generation provided in the above-mentioned first embodiment, and will not be described in detail herein one by one.

[0079] Embodiment Five:

[0080] Please refer to Figure 11 and Figure 12, the pipette tip for digital PCR microdroplet generation provided in Embodiment 5 of the present application will be described. The pipette tip for digital PCR microdroplet generation provided in Embodiment 5 of the present application is an improvement based on the pipette tip for digital PCR microdroplet generation provided in Embodiment 1. The difference between the pipette tip for digital PCR microdroplet generation provided in Embodiment 5 of the present application and the pipette tip for digital PCR microdroplet generation provided in the above Embodiment 1 is that: the pipette tip body 1 is in an inverted conical configuration, the top of the pipette tip body 1 is the fourth liquid inlet 18, and a fourth liquid outlet 19 is provided on the outer side wall of the pipette tip body 1. With this structure, the liquid entering from the fourth liquid inlet 18 can be discharged from the fourth liquid outlet 19, thereby stably generating microdroplets.

[0081] In one embodiment, please refer to Figure 11 and Figure 12 , the number of the fourth liquid outlets 19 can be multiple, and the multiple fourth liquid outlets 19 are symmetrically distributed with respect to the central axis of the pipette tip body 1. With this structure, the pipette tip can generate multiple microdroplets at one time, thereby meeting the requirements of high throughput and having high efficiency. Among them, the number of the fourth liquid outlets 19 can be two, four, etc., and no unique limitation is made here.

[0082] The other structures of the pipette tip for digital PCR microdroplet generation provided in Embodiment 5 of the present application are the same as the corresponding structures of the pipette tip for digital PCR microdroplet generation provided in the above Embodiment 1, and will not be described in detail here.

[0083] Figure 13 is a diagram of the generation of microdroplets by a common pipette tip in a digital PCR experiment. It can be seen from the figure that: the sizes of the microdroplets are not uniform, and many small particle microdroplets are interspersed among the large particle microdroplets. Figure 14 is a diagram of the generation of microdroplets by the pipette tip provided in any one of Embodiments 1 to 5 of the present application in a digital PCR experiment. It can be seen from the figure that: the size uniformity of the microdroplets is good. Therefore, the pipette tip provided in the embodiments of the present application has better size uniformity and better quality of the microdroplets obtained in the digital PCR detection technology compared with the common pipette tip.

[0084] The above are only optional embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A pipette tip for digital PCR microdroplet generation, characterized in that, Comprising: A tip body, the tip body being a hollow tubular structure; the liquid outlet end of the tip body is oval. A plurality of reinforcing ribs, the plurality of reinforcing ribs being annularly and arrayedly distributed on the outer peripheral surface of the tip body, and each of the reinforcing ribs extending from one end of the tip body towards the other end of the tip body.

2. The pipette tip for digital PCR microdroplet generation according to claim 1, characterized in that: One end of the tip body is a first liquid inlet, the other end of the tip body is a first liquid outlet, and the diameter of the tip body gradually decreases from the first liquid inlet towards the first liquid outlet.

3. The pipette tip for digital PCR microdroplet generation according to claim 2, characterized in that: The tip for digital PCR microdroplet generation further includes a connection guide plate mounted at one end of the tip body away from the first liquid outlet, and a through hole communicating with the first liquid inlet is provided on the connection guide plate.

4. The pipette tip for digital PCR microdroplet generation according to claim 3, wherein: The diameter of the through hole gradually decreases from the first liquid inlet towards the first liquid outlet.

5. The pipette tip for digital PCR microdroplet generation according to claim 1, characterized in that: The tip body has a cylindrical configuration, the top of the tip body is a second liquid inlet, the bottom of the tip body is closed, and a second liquid outlet is provided on the tip body.

6. The pipette tip for digital PCR microdroplet generation according to claim 5, characterized in that: The second liquid outlet is provided on the outer side wall of the tip body; Or, the second liquid outlet is provided at the bottom of the tip body.

7. The pipette tip for digital PCR microdroplet generation according to claim 1, characterized in that: The tip body includes a first section having a cylindrical configuration and a second section connected to the first section. Both ends of the first section are open, and the top of the first section is a third liquid inlet; the second section has a hemispherical configuration and protrudes away from the first section, and a third liquid outlet is provided on the tip body.

8. The pipette tip for digital PCR microdroplet generation according to claim 7, wherein: The third liquid outlet is provided on the outer side wall of the first section; Or, the third liquid outlet is provided on the second section.

9. The pipette tip for digital PCR microdroplet generation according to claim 1, wherein: The tip body has an inverted cone configuration, the top of the tip body is a fourth liquid inlet, and a fourth liquid outlet is provided on the outer side wall of the tip body.

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