Anti-aging composition for targeting and activating cellular energy, its preparation method and application

Abstract

The present invention belongs to the technical field of daily chemical products, and relates to an anti-aging composition for targeting and activating cell energy, a preparation method thereof and an application. The composition includes β-nicotinamide mononucleotide, Paeonia lactiflora root extract, Bacillus fermentation product, and Caesalpinia spinosa gum. The composition product of the present invention aims at the energy shortage caused by skin mitochondrial damage, combs and improves mitochondrial dysfunction, activates mitophagy, increases the expression of silent information regulator 3 (SIRT3) in mitochondria, activates cellular oxygen consumption, protects mitochondria from oxidative stress, increases intracellular energy reserve (ATP), rationally combines various components, promotes cell metabolism in multiple dimensions, accelerates skin renewal, realizes targeted anti-aging of the skin, and with the sustained-release effect brought by Caesalpinia spinosa gum, ensures the immediate and long-term anti-aging activity of the composition, improves bioavailability, is mild and non-irritating to the skin, and can be widely applied in the cosmetics industry.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily chemical products, and in particular relates to an anti-aging composition for targeting and activating cell energy, its preparation method and applications. Background Art

[0002] The skin covers the whole body with a total area of 1.5 - 2 square meters. It is an organ with a very strong renewal and metabolism ability, and its efficient metabolism depends on the rapid proliferation of its mother cells. These metabolisms and proliferations of the skin are provided with energy requirements by mitochondrial respiration. From the perspective of cell biology, 90% of the energy generated by intracellular oxidative phosphorylation comes from mitochondria.

[0003] Existing studies believe that the degeneration, leakage and rupture of mitochondria are all important reasons for cell aging. When mitochondrial DNA damage occurs, the generated energy decreases, affecting the energy supply of cells, leading to the decline of cell, tissue and organ functions. There are also studies showing that the mitochondrial physical network in epidermal cells of young skin is highly connected, while the mitochondrial network of the elderly is significantly fragmented, showing a state of poor circulation and excessive phagocytosis of mitochondria.

[0004] Mitochondria are crucial for the normal growth and development of the skin. Mitochondrial damage can lead to skin dysfunction and even aging. Therefore, keeping mitochondria intact is to maintain cell vitality, and having healthy skin cells is to retain youth. How to delay the destruction process of mitochondria has become an urgent problem to be solved in this field.

[0005] At present, there have been a great deal of studies on the mechanism of skin aging, and more than thirty theories have emerged successively. Although mitochondria are very important for the normal growth of the skin, there are few relevant studies on anti-aging of mitochondrial cells. Moreover, there are even fewer skin care products targeting the improvement of mitochondria, and the mechanism is still unclear. For example, Patent CN112891241A discloses a nano-composition for targeted mitochondrial skin anti-aging, which includes β-nicotinamide mononucleotide NMN, other anti-aging active ingredients, and a nano-drug carrier; the NMN accounts for 0.1-10% of the total mass of the nano-composition; the other anti-aging active ingredients include at least one of antioxidants, protein synthesis promoters, anti-photoaging agents, and moisturizers; the raw materials of the nano-drug carrier include cell penetration promoters, emulsifiers, co-emulsifiers, liquid lipids, and water. Among them, the antioxidant includes one or more of vitamin antioxidants and non-vitamin antioxidants, and the protein synthesis promoter includes one or more of signal peptides and non-peptide protein synthesis promoters; the anti-photoaging agent includes one or more of matrix metalloproteinase inhibitors and cell repair agents. Chinese Patent CN113453690A discloses a triple therapy for anti-aging, which effectively eradicates senescent cells and cells carrying senescence-related markers by inhibiting mitochondrial biogenesis during induced mitochondrial oxidative stress without inhibiting normal cells. Embodiments may include a therapeutic agent that inhibits mitochondrial biogenesis and targets large mitochondrial ribosomes, a therapeutic agent that inhibits mitochondrial biogenesis and targets small mitochondrial ribosomes, and a therapeutic agent that acts as a pro-oxidant or induces mitochondrial oxidative stress.

[0006] However, although the prior art disclosed in the above two patents involves mitochondrial cell senescence, the targets are not comprehensive, and it is difficult for conventional antioxidants, anti-photoaging agents, etc. to exert continuous anti-aging effects, with low bioavailability, resulting in weakened effects.

[0007] Therefore, it is of great significance to design an anti-aging product that specializes in mitochondrial cell senescence, has a comprehensive and systematic target, and can continuously exert its effects. Summary of the Invention

[0008] The purpose of the present invention is to provide an anti-aging composition for targeted activation of cell energy, its preparation method and application, so as to overcome the problems of incomplete targets, low bioavailability, and poor persistence of anti-aging effects in the prior art anti-aging compositions.

[0009] The purpose of the present invention can be achieved by the following technical solutions:

[0010] The present invention provides an anti-aging composition for targeted activation of cell energy, which comprises:

[0011] 0 - 90 parts of β - nicotinamide mononucleotide, 0 - 90 parts of Paeonia albiflora root extract, 0 - 90 parts of Bacillus fermentation product, 0 - 20 parts of Caesalpinia spinosa gum;

[0012] And at least three of β - nicotinamide mononucleotide, Paeonia albiflora root extract, Bacillus fermentation product, and Caesalpinia spinosa gum are not 0 at the same time.

[0013] Furthermore, the anti - aging composition for targeted activation of cellular energy contains: 10 - 90 parts of β - nicotinamide mononucleotide, 10 - 90 parts of Paeonia albiflora root extract, 10 - 90 parts of Bacillus fermentation product, and 5 - 20 parts of Caesalpinia spinosa gum.

[0014] Still further, the anti - aging composition for targeted activation of cellular energy contains: 20 - 50 parts of β - nicotinamide mononucleotide, 20 - 50 parts of Paeonia albiflora root extract, 20 - 50 parts of Bacillus fermentation product, and 5 - 20 parts of Caesalpinia spinosa gum.

[0015] Even further, the anti - aging composition for targeted activation of cellular energy contains: 30 parts of β - nicotinamide mononucleotide, 30 parts of Paeonia albiflora root extract, 30 parts of Bacillus fermentation product, and 10 parts of Caesalpinia spinosa gum.

[0016] In the anti - aging composition for targeted activation of cellular energy provided by the present invention, the β - nicotinamide mononucleotide is used to reverse mitochondrial dysfunction.

[0017] In the anti - aging composition for targeted activation of cellular energy provided by the present invention, the Paeonia albiflora root extract is used to activate mitophagy and remove damaged mitochondria.

[0018] In the anti - aging composition for targeted activation of cellular energy provided by the present invention, the Bacillus fermentation product is used to protect mitochondria from oxidative stress.

[0019] In the anti - aging composition for targeted activation of cellular energy provided by the present invention, the Caesalpinia spinosa gum is used to slow - release the efficacy, ensure the immediate and long - term skin - care activity of the composition, and improve the bioavailability.

[0020] The anti - aging composition for targeted activation of cellular energy provided by the present invention can up - regulate the gene expression of sirtuin 3, protect mitochondria from oxidative stress, and at the same time increase cellular energy.

[0021] The anti - aging composition for targeted activation of cellular energy provided by the present invention can targetedly activate cellular energy, further targetedly regulate the function of cellular mitochondria, realize energy supply for cells, and achieve the anti - aging effect.

[0022] The present invention also provides a method for preparing the anti-aging composition for targeted activation of cellular energy, which is specifically as follows:

[0023] The formulated amounts of β-nicotinamide mononucleotide, peony root extract, Bacillus fermentation product, and tsugae gum are fully mixed and stirred evenly to prepare the anti-aging composition for targeted activation of cell energy.

[0024] Furthermore, in the preparation method, the mixing temperature is 20-40° C., and the stirring speed is 200-400 r / min.

[0025] The present invention also provides an application of an anti-aging composition for targeted activation of cell energy, wherein the anti-aging composition for targeted activation of cell energy is used in cosmetics or skin care products.

[0026] Based on the total weight of the cosmetic or skin care product, the cosmetic or skin care product comprises 0.01-50% of an anti-aging composition that targets and activates cellular energy; and 50-99.99% of conventional excipients in the field of skin care products. Preferably, the cosmetic or skin care product comprises 0.01-30% of an anti-aging composition that targets and activates cellular energy; and 70-99.99% of conventional excipients in the field of skin care products. Further preferably, the cosmetic or skin care product comprises 5-20% of an anti-aging composition that targets and activates cellular energy; and 80-95% of conventional excipients in the field of skin care products.

[0027] Furthermore, the conventional auxiliary materials include one or a combination of surfactants, powder materials, thickeners, conditioners, moisturizers, preservatives, chelating agents, and deionized water.

[0028] Surfactants, thickeners, conditioners, moisturizers, preservatives, and chelating agents can be selected from conventional cosmetics. For example, surfactants can be selected from glyceryl stearate, PEG-100 stearate, cetearyl glucoside, etc.; thickeners can be selected from xanthan gum, carbomer, acrylates / C10-30 alkyl acrylate crosspolymer, etc.; conditioners can be selected from white meadowfoam seed oil, squalane, caprylic / capric triglyceride, hydrogenated polydecene, etc.; moisturizers can be selected from polyols, sodium hyaluronate, betaine, trehalose, etc., and sodium hyaluronate and glycerin are used together to enhance moisturizing effect and save costs; preservatives can be selected from phenoxyethanol, p-hydroxyacetophenone, polyols, etc., among which p-hydroxyacetophenone and hexylene glycol can enhance the preservative effect; chelating agents can be selected from EDTA salts, etc.

[0029] The present invention also provides a cosmetic or skin care product, which, based on the total weight of the cosmetic or skin care product, contains 0.01-50% of the anti-aging composition for targeting and activating cellular energy; and 50-99.99% of excipients for cosmetics or skin care products; preferably, the cosmetic or skin care product contains 0.01-30% of the anti-aging composition for targeting and activating cellular energy; and 70-99.99% of conventional excipients in the field of skin care products. More preferably, the cosmetic or skin care product contains 5-20% of the anti-aging composition for targeting and activating cellular energy; and 80-95% of conventional excipients in the field of skin care products.

[0030] The excipients for the cosmetic or skin care product include one or a combination of several of surfactants, powder materials, thickeners, conditioning agents, moisturizers, preservatives, chelating agents, deionized water.

[0031] Furthermore, the present invention also provides a method for preparing a skin care product or a cosmetic, comprising the following steps:

[0032] Pre-disperse an alcohol-based moisturizer, a chelating agent and a thickener evenly, and then add them to deionized water and stir until completely dissolved;

[0033] Add part of the non-alcohol-based moisturizer, stir at 100-200 r / min to disperse it evenly, then heat to 80-90 °C, add a preservative and stir evenly, and stop heating after complete dissolution;

[0034] Cool to 35-40 °C, add the anti-aging composition for targeting and activating cellular energy, and stir evenly;

[0035] Stir the remaining non-alcohol-based moisturizer and the alcohol-based preservative evenly, add them to the system, and stir evenly to obtain the skin care product or the cosmetic.

[0036] Furthermore, the present invention also provides a method for preparing a serum, comprising the following steps:

[0037] Pre-disperse 1,3-butanediol, EDTA-2Na and xanthan gum evenly, and then add them to deionized water and stir until completely dissolved;

[0038] Add trehalose and stir at 150 r / min to disperse it evenly, then heat to 85 °C, add p-hydroxyacetophenone and stir evenly, and stop heating after complete dissolution;

[0039] Cool to 38 °C, add the anti-aging composition for targeting and activating cellular energy, and stir evenly;

[0040] Stir glycerol and 1,2-hexanediol evenly, add them to the system, and stir evenly to obtain the serum.

[0041] Furthermore, the cosmetics or skin care products are made into various cosmetics such as anti-aging facial cleanser, anti-aging cream, anti-aging lotion, anti-aging toner, anti-aging mask, anti-aging eye cream or anti-aging gel.

[0042] Furthermore, the cosmetics or skin care products are particularly suitable for the skin of people such as those with staying-up-late type and aging type.

[0043] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0044] Aiming at the problem that the damage of skin cell mitochondria leads to insufficient cell energy, the present invention starts from improving mitochondrial dysfunction, activating mitochondrial autophagy, increasing the expression of silent information regulator 3 (SIRT3) in mitochondria, activating cell oxygen consumption, protecting mitochondria from oxidative stress, and increasing intracellular energy reserve (ATP), etc., and reasonably collocates various components to promote cell metabolism in multiple dimensions, accelerate skin renewal, and achieve targeted anti-aging of the skin.

[0045] The targeted anti-aging referred to in the present invention means to specifically improve mitochondrial dysfunction, activate mitochondrial autophagy, increase the expression of silent information regulator 3 (SIRT3) in mitochondria, activate cell oxygen consumption, protect mitochondria from oxidative stress, and increase intracellular energy reserve (ATP), so as to achieve anti-aging of the skin.

[0046] The anti-aging composition for targeted activation of cell energy of the present invention helps to up-regulate the expression of sirtuin 3 gene, can protect mitochondria from oxidative stress, and at the same time increases cell energy (ATP). The increase in ATP helps to up-regulate the expression of talin 1 gene, increases the cross-linking of actin-myosin, strengthens the adhesion of cells to the ECM, and produces the effect of lifting and tightening.

[0047] The anti-aging composition for targeted activation of cell energy of the present application contains tara gum, which can play a sustained-release effect, ensure the immediate and long-term anti-aging activity of the composition, improve the bioavailability, and the anti-aging composition of the present application is mild and non-irritating to the skin and can be widely applied in cosmetics or skin care products. It has important practical significance and broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 The data graph of the change in water content of the stratum corneum provided by the present invention.

[0049] Figure 2 The improvement diagram of the dermis layer of the skin provided by the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0050] For ease of understanding, exemplary descriptions of concepts related to this application are provided for reference. It can be understood that the descriptions of related concepts are also part of the embodiments of this application, as follows:

[0051] [1]ATP

[0052] Adenosine triphosphate (ATP), an unstable high-energy compound, consists of 1 molecule of adenine, 1 molecule of ribose, and 3 molecules of phosphate. When hydrolyzed, it releases a relatively large amount of energy and is the most direct energy source in living organisms.

[0053] [2]NAD+

[0054] Chinese name: Nicotinamide adenine dinucleotide, abbreviated as coenzyme I. It is an electron transfer agent and the coenzyme of many dehydrogenases in the body, connecting the tricarboxylic acid cycle and the respiratory chain. Its function is to transfer the hydrogen removed during metabolism to flavoprotein. NADH or more precisely NADH +, H+ is its reduced form.

[0055] NAD+ is an essential substance for activating acetylase and decreases with age.

[0056] [3]Mitophagy

[0057] Mitophagy is the process of self-degradation of damaged mitochondria. Some degradation fragments are recycled, and through the mitochondrial regeneration mechanism, new mitochondria are generated, which is like the recycling of cells.

[0058] [4]ROS

[0059] Abbreviation for Reactive oxygen species, reactive oxygen. Harmful compounds produced during the oxidation reaction of the body, with strong oxidizing properties, can damage the tissues and cells of the body, thereby causing chronic diseases and aging effects. [[ID={29]]

[0060] [5]ECM

[0061] Extracellular matrix, ECM, is a macromolecule secreted by cells into the extracellular matrix, forming a complex network structure that supports and connects tissue structures and regulates tissue development and cell physiological activities.

[0062] [6]SIRT3

[0063] The silent regulator factor SIRT3 is one of the 7 members of the "sirtuin family" in mammals. It is a NAD-dependent histone deacetylase and is mainly present in mitochondria. Through its enzymatic catalytic activity, SIRT3 can not only regulate life processes such as the body's energy metabolism, cell aging, and tumor formation, but also play a crucial regulatory role in apoptosis.

[0064] To make the objectives, technical solutions, and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other implementation manners obtained by those of ordinary skill in the art without creative efforts shall fall within the scope protected by the present invention.

[0065] The present invention discloses an anti-aging composition for targeting and activating cellular energy, which is composed of nicotinamide mononucleotide, peony root extract, bacillus fermentation product, and tara gum as raw materials and combined in a suitable weight ratio.

[0066] The following are the technical efficacy descriptions of the raw material components respectively.

[0067] Nicotinamide mononucleotide

[0068] Nicotinamide mononucleotide: Abbreviated as "NMN", it is a bioactive nucleotide composed of a phosphate group, a nicotinamide, and ribose, and is an inherent substance in the human body. NMN has two irregular forms of existence, α and β, and the β isomer is the active form of NMN.

[0069] In the human body, NMN is the most direct precursor of NAD+, and can participate in the biosynthesis of NAD+. As a key coenzyme, NAD+ / NADH serves as an enzyme cofactor for mediating electron transfer in the oxidative-reduction metabolic reactions in mitochondria, and mitochondria are the main sites of energy metabolism. By increasing the level of NAD+ in the body and supplementing the loss of NAD+, more ATP can be generated to maintain cellular energy, thereby delaying aging and improving functions. Nicotinamide mononucleotide is mainly used in the composition of this application to improve mitochondrial dysfunction.

[0070] Peony root extract

[0071] The said peony root extract is extracted from the roots of peony (PAEONIA ALBIFLORA) and is rich in the active ingredient paeoniflorin. In the present invention, the peony root extract is used to activate mitophagy and remove damaged mitochondria.

[0072] Mitochondrial aging is one of the main reasons for the decline of cell function. Paeonia root extract, through the mechanism of mitophagy, when the autophagosome completely wraps and isolates the damaged mitochondria, lysosomes will degrade them. Some fragments will be cleared, and others will be reused, thus optimizing the cell energy efficiency.

[0073] Bacillus fermentation product

[0074] The Bacillus fermentation product refers to the fermentation product obtained after Bacillus fermentation and centrifugal filtration. Generally, Bacillus collected at Reunion Island (depth 3400 meters, temperature 1.5 °C, oxygen content 3.8 μmol / L) is preferably used. After fermentation to the optimal concentration, the fermentation product is obtained by centrifugal filtration.

[0075] Tara gum

[0076] The tara gum refers to the gum extracted from a shrub in South America known as "Inca green gold", Caesalpinia spinosa, which is rich in galactomannan - a high - molecular - weight polysaccharide composed of linear mannose and galactose, with a mannose - to - galactose ratio of 3:1, forming a 3D colloid. The tara gum can be obtained through purchase channels.

[0077] Tara gum is an interpenetrating polysaccharide network with a porous 3D structure, which can slowly release the active molecules encapsulated therein, and the sustained release optimizes and prolongs the immediate and long - term effects of the active ingredients.

[0078] The anti - aging composition for targeted activation of cell energy in the present invention aims at the problem of insufficient cell energy caused by mitochondrial damage in skin cells. Starting from improving mitochondrial dysfunction, activating mitophagy, increasing the expression of sirtuin 3 (SIRT3) in mitochondria, activating cell oxygen consumption, protecting mitochondria from oxidative stress, and increasing intracellular energy reserve (ATP), etc., various components are rationally combined to promote cell metabolism in multiple dimensions, accelerate skin renewal, and achieve targeted anti - aging of the skin. With the sustained - release effect brought by tara gum, it ensures the immediate and long - term anti - aging activity of the composition, improves bioavailability, is gentle and non - irritating to the skin, and can be widely used in the cosmetics industry.

[0079] In the following examples, the unit of parts by weight is g. All raw material components of the present invention are commercially available products. β - Nicotinamide mononucleotide is purchased from Shenzhen Vicki Biotechnology Co., Ltd., Paeonia root extract and tara gum are both purchased from PROVITAL Company, and the Bacillus fermentation product is purchased from LIPOTRUE Company.

[0080] Example 1

[0081] This embodiment provides an anti-aging essence for targeting and activating cellular energy. The raw material components of the essence include:

[0082] 5% of an anti-aging composition for targeting and activating cellular energy, 0.2% of xanthan gum, 3% of 1,3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1,2-hexanediol, and the balance being deionized water.

[0083] Among them, based on the total weight of the anti-aging composition for targeting and activating cellular energy, the raw material components of the composition include:

[0084] 33.4 parts of β-nicotinamide mononucleotide, 33.3 parts of peony root extract, 33.3 parts of Bacillus fermentation product, and 0 parts of Caesalpinia spinosa gum.

[0085] The anti-aging essence for targeting and activating cellular energy is prepared by the following method:

[0086] Pre-disperse 1,3-butanediol, EDTA-2Na and xanthan gum evenly, then add them to deionized water and stir until completely dissolved; add trehalose and stir at 150 r / min to disperse it evenly, then heat to 85 °C and add p-hydroxyacetophenone and stir evenly. Stop heating after complete dissolution; cool to 38 °C, add the anti-aging composition for targeting and activating cellular energy, stir evenly, stir glycerol and 1,2-hexanediol evenly, add them to the system, stir evenly and then discharge and package to obtain the anti-aging essence for targeting and activating cellular energy.

[0087] Example 2

[0088] This embodiment provides an anti-aging essence for targeting and activating cellular energy. The raw material components of the essence include:

[0089] 5% of an anti-aging composition for targeting and activating cellular energy, 0.2% of xanthan gum, 3% of 1,3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1,2-hexanediol, and the balance being deionized water.

[0090] Among them, based on the total weight of the anti-aging composition for targeting and activating cellular energy, the raw material components of the composition include: 30 parts of β-nicotinamide mononucleotide, 30 parts of peony root extract, 30 parts of Bacillus fermentation product, and 10 parts of Caesalpinia spinosa gum.

[0091] The anti-aging essence for targeting and activating cellular energy is prepared by the following method:

[0092] Pre-disperse 1,3-butanediol, EDTA-2Na and xanthan gum evenly, then add them to deionized water and stir until completely dissolved; add trehalose and stir at 150 r / min to make it disperse evenly, then heat to 85 °C and add p-hydroxyacetophenone and stir evenly. Stop heating after complete dissolution; cool to 38 °C and add the anti-aging composition that targets and activates cellular energy, stir evenly, stir glycerol and 1,2-hexanediol evenly, add them to the system, stir evenly and then discharge and package to obtain the anti-aging essence that targets and activates cellular energy.

[0093] Example 3

[0094] This example provides an anti-aging essence that targets and activates cellular energy. The raw material components of this essence include:

[0095] 10% of the anti-aging composition that targets and activates cellular energy, 0.2% of xanthan gum, 3% of 1,3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1,2-hexanediol, and the balance is deionized water.

[0096] Among them, based on the total weight of the anti-aging composition that targets and activates cellular energy, the raw material components of the composition include: 30 parts of β-nicotinamide mononucleotide, 30 parts of peony root extract, 30 parts of Bacillus fermentation product, and 10 parts of tara gum.

[0097] The anti-aging essence that targets and activates cellular energy is prepared by the following method:

[0098] Pre-disperse 1,3-butanediol, EDTA-2Na and xanthan gum evenly, then add them to deionized water and stir until completely dissolved; add trehalose and stir at 150 r / min to make it disperse evenly, then heat to 85 °C and add p-hydroxyacetophenone and stir evenly. Stop heating after complete dissolution; cool to 38 °C and add the anti-aging composition that targets and activates cellular energy, stir evenly, stir glycerol and 1,2-hexanediol evenly, add them to the system, stir evenly and then discharge and package to obtain the anti-aging essence that targets and activates cellular energy.

[0099] Blank Example

[0100] The difference between this blank example and Example 1 is only that the raw material components of the anti-aging essence that targets and activates cellular energy are different. The essence in this blank example does not contain the anti-aging composition that targets and activates cellular energy, and other operations are the same as those in Example 1.

[0101] Effect Verification Test Example

[0102] I. In vitro test

[0103] In in vitro tests, anti-aging compositions with different components that target the activation of cellular energy were used as comparative examples for experimentation. The raw material components of the anti-aging compositions in Comparative Examples 1-8 were different, and the formulations are shown in Table 1.

[0104] Table 1 Formulations of the raw material components in Comparative Examples 1-8

[0105]

[0106] Materials and methods: Human normal skin (54 years old, with the consent of the patient) remaining after hospital surgery was taken, and isolation and culture were carried out using the tissue block method. Fibroblasts in a stable growth state were taken for the experiment.

[0107] Extraction of mitochondria: Homogenates were prepared from cells in a stable growth state, the cells were ruptured by mechanical methods, and excess residues and large organelles were removed by low-speed differential centrifugation. Mitochondria were obtained by high-speed differential centrifugation. To ensure mitochondrial activity, the operation was carried out in an ice bath.

[0108] Experimental grouping: Randomly divided into an experimental group and a control group, with 6 parallel groups set in each group, and the results were averaged. The experimental group was divided into 8 subgroups, and the compositions of Comparative Examples 1-8 diluted 1000 times were added respectively. The control group was a complete medium, and all experimental operations were the same.

[0109] Observation indicators:

[0110] (1) Detection of cellular ROS content: DCF method was used to measure ROS. The DCFH-DA fluorescent probe (purchased from Yeasen, product number: 50101ES01) was used to detect the cellular ROS content. The excitation wavelength was 480 nm and the emission wavelength was 525 nm. The detection was carried out specifically according to the probe instruction manual.

[0111] (2) Detection of mitochondrial SIRT3 activity: Fluorescence method was used for measurement. For mitochondrial experiments, it was carried out according to the instruction manual of the SIRT3 activity detection kit (Abcam, USA, product number: ab156067).

[0112] (3) Measurement of mitochondrial ATP production: After the samples and the control group were processed, a Seahorse XF 96 extracellular flux analyzer (Agilent, USA) was used for detection. The specific experimental steps were carried out according to the instruction manual of the cell mitochondrial stress detection kit (product number: 103015-100) of Seahorse Bioscience.

[0113] 1.1 Detection of cellular ROS content

[0114] Hypoxia causes mitochondria to produce excessive ROS, ultimately leading to cell dysfunction. Therefore, the clearance of ROS can produce important cell protection effects.

[0115] DCFH-DA itself has no fluorescence and can freely cross the cell membrane. After entering the cell, it can be hydrolyzed by intracellular esterases to generate DCFH, and DCFH cannot permeate the cell membrane. Therefore, the probe is easily accumulated in the cell. Intracellular reactive oxygen species can oxidize non-fluorescent DCFH to generate fluorescent DCF. The intensity of green fluorescence is proportional to the level of reactive oxygen species. According to the fluorescence signal intensity, the true level of reactive oxygen species can be analyzed.

[0116] The relative fluorescence intensity values of ROS of the compositions of Comparative Examples 1-8 are shown in Table 2.

[0117] Table 2 Relative fluorescence intensity of ROS after treatment with the compositions of Comparative Examples 1-8

[0118] Comparative Example ROS Relative Fluorescence Intensity Comparative Example 1 60.90％ Comparative Example 2 71.50％ Comparative Example 3 69.80％ Comparative Example 4 68.30％ Comparative Example 5 78.70％ Comparative Example 6 76.10％ Comparative Example 7 80.40％ Comparative Example 8 63.10％ Control Group 99.69％

[0119] Compared with the control group, the mitochondrial ROS fluorescence intensity decreased significantly after treatment with the compositions of Comparative Examples 1-8, indicating that the compositions of Comparative Examples 1-8 of the present invention all have the efficacy of scavenging ROS and protecting cells. At the same time, the relative fluorescence intensity of ROS in Comparative Example 1 < Comparative Example 8 < Comparative Examples 2, 3, 4 < Comparative Examples 5, 6, 7, proving that the anti-aging cosmetic composition for targeted activation of cell energy provided by the present invention simultaneously contains β-nicotinamide mononucleotide, Paeonia albiflora root extract, Bacillus fermentation product and Caesalpinia spinosa gum, and the combined application of these components has a synergistic effect.

[0120] 1.2 Mitochondria promote the expression of silent regulator factor SIRT3

[0121] The "longevity factor" Sirtuins are NAD+-dependent deacetylases that play a key role in cell metabolism and environmental stress. The up-regulation of SIRT3 gene expression can increase cell energy while protecting mitochondria from oxidative stress. Through its enzymatic catalytic activity, SIRT3 can not only regulate life processes such as body energy metabolism, cell aging and tumor formation, but also play a crucial regulatory role in cell apoptosis.

[0122] The improvement values of the promotion of silent regulator factor SIRT protein expression by the compositions of Comparative Examples 1-8 are shown in Table 3.

[0123] Taking Comparative Example 1 as an example, after treatment with the compositions of Comparative Example 1 and the control group, the average relative expression level of SIRT3 in the control group was 0.998, and the average relative expression level of SIRT3 in the Comparative Example 1 group was 1.186. The improvement rate of the SIRT3 protein expression level in Comparative Example 1 compared to the control group was +18.8%.

[0124] Table 3 Improvement rates of the compositions of Comparative Examples 1-8 on the SIRT3 protein expression level

[0125] Comparative Example Improvement Rate of SIRT3 Protein Expression Level Comparative Example 1 +18.8％ Comparative Example 2 +14.7％ Comparative Example 3 +16.4％ Comparative Example 4 +13.0％ Comparative Example 5 +9.2％ Comparative Example 6 +7.3％ Comparative Example 7 +11.8％ Comparative Example 8 +17.1％

[0126] Compared with the control group, after treatment with the compositions of Comparative Examples 1-8, the SIRT3 protein expression level increased significantly, indicating that the compositions of Comparative Examples 1-8 of the present invention all have the effect of activating the expression of the silent regulator SIRT3. At the same time, the SIRT3 protein expression level: Comparative Example 1 > Comparative Example 8 > Comparative Examples 2, 3, 4 > Comparative Examples 5, 6, 7, proving that the anti-aging cosmetic composition for targeting the activation of cellular energy provided by the present invention simultaneously contains β-nicotinamide mononucleotide, Paeonia albiflora root extract, Bacillus fermentation product, and Caesalpinia spinosa gum, and the combined application of these components has a synergistic effect.

[0127] 1.3 ATP production in mitochondria

[0128] Cells must have an energy supply to be active. Mitochondria are the organs in cells that produce energy ATP and are called the power plants of cells. ATP content determination can be used to detect the ATP level in cells and can reflect the activity of mitochondria.

[0129] The improvement values of the compositions of Comparative Examples 1-8 in promoting cellular energy ATP production are shown in Table 4. Among them, the improvement value of the ATP synthesis rate of any comparative example = (the average ATP production of this comparative example - the average ATP production of the control group) / the average ATP production of the control group * 100%.

[0130] Taking Comparative Example 1 as an example, after treatment with the compositions of Comparative Example 1 and the control group, the average ATP production of the control group was 97.98 pmol / (min·μg), and the average ATP production of Comparative Example 1 was 160.88 pmol / (min·μg). The improvement rate of the ATP production in Comparative Example 1 compared to the control group was +64.2%.

[0131] Table 4 Improvement values of the compositions of Comparative Examples 1-8 on the ATP production in mitochondria

[0132] Comparative Example Improvement Value of ATP Synthesis Rate Comparative Example 1 +64.2％ Comparative Example 2 +52.9％ Comparative Example 3 +55.7％ Comparative Example 4 +58.3％ Comparative Example 5 +33.6％ Comparative Example 6 +28.4％ Comparative Example 7 +25.5％ Comparative Example 8 +62.9％

[0133] It can be seen from the experimental data in Table 4 that the improvement values of the synthesis rate of ATP in mitochondria are Comparative Example 1 > Comparative Example 8 > Comparative Examples 2, 3, 4 > Comparative Examples 5, 6, 7, which proves that the synergistic effect between the raw material components of the anti-aging composition for targeted activation of cellular energy has the effect of activating mitochondria to produce more energy than a simple composition of one or two ingredients.

[0134] 2. Human Testing

[0135] Test Method

[0136] 1. Testers: 80 healthy women (23 to 50 years old, average age 39.6 years old) were randomly divided into 4 groups, each with 20 people. They used Example 1, Example 2, Example 3, and the blank product respectively. The testers in the same group used the same formula product.

[0137] 2. Test area: face.

[0138] 3. Usage: Use in the morning and evening, twice a day, for 4 weeks, and avoid outdoor exposure.

[0139] 4. Test conditions: The subjects rest quietly for 30 minutes in an environment with a temperature of 21±1°C and a relative humidity of 50±5%.

[0140] 5. Test parameters:

[0141] The Finnish Delfin skin tissue moisture content tester measures the moisture content of the skin surface. The higher the moisture content, the better the skin.

[0142] German UItrascan UC22 skin ultrasound diagnostic instrument is used for facial image acquisition and dermis analysis.

[0143] Consumer self-assessment.

[0144] 6. Test cycle: before use (D0), 0.5-8 hours of use, 14 days of use (D14), 28 days of use (D28)

[0145] 1) Improved skin moisture content

[0146] The serums obtained in Example 1, Example 2 and Example 3 were tested for their effects on improving skin moisture content over 8 hours. The test area was the intersection of the outer corner of the eye and the lower part of the nose (random side). Figure 1 ( Figure 1The figure is drawn based on the average test results of 20 people in each group). The human body detection results show that, compared with the blank example, for Cosmetic Examples 1, 2, and 3 of the present invention, the skin moisture content is significantly increased 0.5 - 8 hours after use, indicating that the cosmetics containing the anti-aging composition of the present invention have good immediate and 8-hour long-lasting moisturizing effects. At the same time, compared with Example 1, Example 2 has better immediate moisturizing and long-lasting water-locking abilities, indicating that the addition of four components in the anti-aging composition of the present invention has a better long-lasting moisturizing effect than the addition of three components, proving the sustained-release effect brought by the combination of β-nicotinamide mononucleotide, Paeonia lactiflora root extract, Bacillus fermentation product and Caesalpinia spinosa gum in the composition of the present invention, ensuring the immediate and long-lasting anti-aging activity of the composition, improving the bioavailability, and having a good synergistic effect.

[0147] 2) Dermal layer skin quality analysis

[0148] The essence obtained from Example 1, Example 2, Example 3, and the blank example was used for skin dermal layer analysis test with a skin ultrasonic diagnostic instrument. The test area was the intersection of the outer corner of the eye and the corner of the mouth (random side). Table 5 shows the improvement results of the dermal layer thickness and density of the testers of Example 1, Example 2, Example 3, and the blank example at different time points. Figure 2 Figure shows the pictures of the dermal layer thickness and density of a tester in Example 3. Dermal thickness is a dimension for evaluating skin aging. As aging progresses, macromolecular substances in the dermis are lost, the supporting effect of the dermal layer decreases, and the dermal thickness becomes thinner. Dermal density is to measure the content degree of macromolecules in the dermis. Since ultrasonic waves are blocked by macromolecular substances and produce echo, which is called the acoustic impedance coefficient physically, the density measurement is used to evaluate whether the tested sample has an effect on the dermal layer. From Table 5 and Figure 2 it can be seen that both the thickness and density (fluorescent part) of the dermal layer are improved after using the sample of Example 3.

[0149] Compared with the blank example, the dermal thickness of Example 1, Example 2, and Example 3 becomes larger, and the dermal density value becomes larger, indicating that the skin thickness and skin density are improved, confirming that the composition of the present invention has anti-aging effects.

[0150] Table 5 Improvement effects of dermal layer thickness and density after 28 days of use The data of the dermal layer thickness and dermal layer density in Table 5 can be directly read from the instrument.

[0151] 3) Results of consumer satisfaction survey

[0152] The consumer satisfaction survey was conducted by means of a questionnaire. In this test, the agreement degree was measured using a 5-point scale, namely: from 1 to 5 points, where 1 point indicates dissatisfaction, 2 points indicates relatively dissatisfied, 3 points indicates average, 4 points indicates relatively satisfied, and 5 points indicates satisfied. Satisfaction = (Number of subjects with a score ≥ 4 points) ÷ (Total number of valid subjects) × 100%. According to the literature guidance of the method source, the test result requires that the percentage of agreeing consumers be greater than 50% to support the corresponding claim.

[0153] As can be seen from Table 6, the anti-aging essence for targeted activation of cell energy obtained in Examples 1-3 of the present invention has good synergistic anti-aging effects, and the effect is better than that of the essence of the anti-aging composition without targeted activation of cell energy in the blank example. At the same time, the product is mild and non-irritating.

[0154] Table 6 Self-evaluation (Satisfaction) of subjects after 28 days of use

[0155]

[0156] In summary, the anti-aging composition for targeted activation of cell energy provided by the present invention is designed specifically for the energy deficiency caused by mitochondrial damage in skin cells. Starting from improving mitochondrial dysfunction, activating mitophagy, increasing the expression of silent information regulator 3 (SIRT3) in mitochondria, activating cellular oxygen consumption, protecting mitochondria from oxidative stress, and increasing intracellular energy reserve (ATP), various components are rationally combined to promote cell metabolism in multiple dimensions, accelerate skin renewal, and achieve targeted anti-aging of the skin. The anti-aging composition for targeted activation of cell energy in the present application contains tara gum, which can play a sustained-release effect, ensure the immediate and long-term anti-aging activity of the composition, improve bioavailability, and the anti-aging composition of the present application is mild and non-irritating to the skin and can be widely applied in cosmetics or skin care products.

[0157] The above description of the embodiments is to enable those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative efforts. Therefore, the present invention is not limited to the above embodiments, and all improvements and modifications made by those skilled in the art without departing from the scope of the present invention should be within the protection scope of the present invention.

Claims

1. An anti-aging composition that targets and activates cellular energy, characterized in that, The anti-aging composition for targetedly activating cell energy comprises: 10-90 parts of β-nicotinamide mononucleotide, 10-90 parts of peony root extract, 10-90 parts of Bacillus fermentation product, and 5-20 parts of Caesalpinia spinosa gum.

2. The anti-aging composition for targetedly activating cell energy according to claim 1, wherein, The anti-aging composition for targetedly activating cell energy comprises: 20-50 parts of β-nicotinamide mononucleotide, 20-50 parts of peony root extract, 20-50 parts of Bacillus fermentation product, and 5-20 parts of Caesalpinia spinosa gum.

3. The anti-aging composition for targeting and activating cellular energy according to claim 2, characterized in that, The anti-aging composition for targetedly activating cell energy comprises: 30 parts of β-nicotinamide mononucleotide, 30 parts of peony root extract, 30 parts of Bacillus fermentation product, and 10 parts of Caesalpinia spinosa gum.

4. A method for preparing an anti-aging composition that targets and activates cellular energy according to any one of claims 1 to 3, characterized in that, Mix the β-nicotinamide mononucleotide, peony root extract, Bacillus fermentation product, and Caesalpinia spinosa gum in the formula amounts sufficiently and stir evenly to obtain the anti-aging composition for targetedly activating cell energy.

5. The preparation method of the anti-aging composition for targeting and activating cellular energy according to claim 4, characterized in that, The temperature of the mixing is 20-40 °C, and the speed of the stirring is 200-400 r / min.

6. Use of the anti-aging composition for targetedly activating cell energy according to any one of claims 1-3 in the preparation of cosmetics, characterized in that Based on the total weight of the cosmetics, the cosmetics comprise 0.01-50% of the anti-aging composition for targetedly activating cell energy; and 50-99.99% of cosmetic adjuvants; The cosmetic adjuvants include one or a combination of several of surfactants, powder materials, thickeners, conditioning agents, humectants, preservatives, chelating agents, and deionized water.

7. A cosmetic, characterized in that, Based on the total weight of the cosmetics, the cosmetics comprise 0.01-50% of the anti-aging composition for targetedly activating cell energy according to any one of claims 1-3; and 50-99.99% of cosmetic adjuvants; The cosmetic adjuvants include one or a combination of several of surfactants, powder materials, thickeners, conditioning agents, humectants, preservatives, chelating agents, and deionized water.

8. A cosmetic according to claim 7, characterized in that, The cosmetics comprise 0.01-30% of the anti-aging composition for targetedly activating cell energy; and 70-99.99% of cosmetic adjuvants.

9. A cosmetic according to claim 8, characterized in that, The cosmetics comprise 5-20% of the anti-aging composition for targetedly activating cell energy; and 80-95% of cosmetic adjuvants.

10. A cosmetic according to claim 7, characterized in that, The cosmetics are selected from facial cleansers, creams, lotions, toners, masks, or eye creams.

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