A protease crude extract collection system and method

Abstract

The application discloses a kind of protease crude extract collection system and method, in its system: the bottom of circulation tank is communicated with the bottom of buffer tank, and the first inner cavity is communicated with the pump inlet of first pump body, the pump outlet of second pump body, the pump outlet of first pump body, the pump inlet of second pump body are communicated with the inlet end of first three-way valve, the first outlet end of first three-way valve is communicated with the bottom of fourth inner cavity of circulation tank, the second outlet end of first three-way valve is communicated with the second outlet end of second three-way valve, the inlet end of second three-way valve is communicated with the bottom of third inner cavity of circulation tank, and first circulation branch pipe and second circulation branch pipe communicated into the inner cavity of circulation tank are provided on circulation tank;Collecting tank is communicated with the bottom of second inner cavity of circulation tank, the first outlet end of second three-way valve, and its bottom is communicated with discharge end.The application can complete the automation collection of two kinds of protease crude extract synchronously, and can customize increase and decrease the kind of collected protease, and control processing capacity pertinently.

Description

Technical Field

[0001] This invention relates to the field of protease extraction equipment technology, and in particular to a protease crude extract collection system and method. Background Technology

[0002] Male urine contains proteases such as urokinase and ulinastatin. A common method for extracting proteases from urine is the adsorption method. Taking urokinase as an example, the specific process flow is as follows:

[0003] S1. Collect male urine through public toilets in schools, factories, etc. The freshness of the urine will affect the activity of urokinase, so it must be processed in time. Collect and process the urine from the toilet at regular intervals every day, adjust the pH value of the urine to 9, let it stand for 1 hour, siphon the supernatant and discard the grayish-white precipitate.

[0004] S2. Adsorption: Adjust the pH of the siphon supernatant to 5.8-6 with 5N hydrochloric acid (HCl), add 1% (WIV) resin, stir and adsorb for 1 hour, stop stirring, allow the resin to settle naturally, siphon off the residual urine, and collect the resin. Wash the collected resin three times with tap water, then wash it three more times with deionized water until the urine is colorless;

[0005] S3, Elution: First elution: Elute with 2% ammonium hydroxide and 1M sodium chloride solution at 35% volume for 1 hour; Second elution: Elute with the same volume of the same solution for 30 minutes, and start collecting urokinase.

[0006] S4. After the finished urokinase is sent to a testing unit to test its activity, it can be sold to crude product purchasers or biochemical pharmaceutical companies.

[0007] In existing processes for extracting proteases from urine, most steps are performed manually, especially urine collection and protease adsorption, resulting in low efficiency. Furthermore, in the adsorption processes of some existing extraction devices, the adsorbent material tends to settle at the bottom of the urine, limiting its adsorption capacity for proteases. In addition, current extraction equipment cannot simultaneously extract two types of proteases and does not consider adapting the processing volume to the actual urine collection volume. Summary of the Invention

[0008] The purpose of this invention is to provide a system and method for collecting crude protease extracts, which can simultaneously and automatically collect two types of crude protease extracts, and can customize the types of proteases collected and control the processing volume accordingly, in view of the existing technical status.

[0009] To achieve the above objectives, the present invention adopts the following technical solution:

[0010] A crude protease extract collection system includes a buffer tank, a circulation tank, a collection tank, a first pump body and a second pump body, a first three-way valve and a second three-way valve;

[0011] The buffer tank is used for urine storage. The buffer tank is connected to the discharge end through a first drain pipe, and a first electric valve is provided on the first drain pipe. A first liquid level sensor is provided on the buffer tank to monitor the liquid level inside the buffer tank.

[0012] The circulation chamber is used for protease adsorption. The inner cavity of the circulation chamber is provided with a first slot, a second slot, a third slot, a fourth slot, a fifth slot, a sixth slot, and a seventh slot from left to right. Specifically: partitions are inserted into the second slot, the fourth slot, and the sixth slot to divide the inner cavity of the circulation chamber into a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity in sequence; or partitions are inserted into the first slot, the third slot, the fourth slot, the fifth slot, and the seventh slot to divide the inner cavity of the circulation chamber into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, a fourth inner cavity, and a second empty cavity in sequence; or partitions are inserted into the first slot, the third slot, the fourth slot, and the fifth slot to divide the inner cavity of the circulation chamber into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity in sequence.

[0013] The circulation tank is lower than the buffer tank. The bottom of the first inner cavity of the circulation tank is connected to the bottom of the buffer tank through the first inlet pipe, and the first inlet pipe is equipped with a second electric valve. The bottom of the first inner cavity of the circulation tank is connected to the discharge end through the second drain pipe, and the second drain pipe is equipped with a third electric valve. The circulation tank is equipped with a first automatic feeding device and a second automatic feeding device, which are used to feed different adsorbent materials into the second inner cavity and the third inner cavity of the circulation tank, respectively. The circulation tank is equipped with a second liquid level sensor to monitor the liquid level in the circulation tank.

[0014] The first inner cavity of the circulation box is connected to the pump inlet of the first pump body and the pump outlet of the second pump body. The pump outlet of the first pump body and the pump inlet of the second pump body are connected to the inlet end of the first three-way valve. The first outlet end of the first three-way valve is connected to the bottom of the fourth inner cavity of the circulation box. The second outlet end of the first three-way valve is connected to the second outlet end of the second three-way valve. The inlet end of the second three-way valve is connected to the bottom of the third inner cavity of the circulation box. The circulation box is provided with a first circulation branch pipe connected to its second inner cavity. One end of the first circulation branch pipe located in the second inner cavity is provided with a spray head. The other end of the first circulation branch pipe is connected to the pump outlet of the second pump body and is provided with a fourth electric valve. The circulation box is provided with a second circulation branch pipe connected to its third inner cavity. One end of the second circulation branch pipe located in the third inner cavity is provided with a spray head. The other end of the second circulation branch pipe is connected to the first outlet end of the first three-way valve and is provided with a fifth electric valve.

[0015] The collection box is used for collecting adsorbent material. The collection box is lower than the circulation box. The collection box is connected to the bottom of the second inner cavity of the circulation box through a first collection branch pipe, and a sixth electric valve is provided on the first collection branch pipe. The collection box is connected to the first outlet end of the second three-way valve through a second collection branch pipe, and a seventh electric valve is provided on the second collection branch pipe. The collection box is equipped with a first filter device that separates the urine discharged from the second inner cavity of the circulation box into the collection box from the corresponding adsorbent material, and a second filter device that separates the urine discharged from the third inner cavity of the circulation box into the collection box from the corresponding adsorbent material. The bottom of the collection box is connected to the discharge end.

[0016] When collecting two proteases using two adsorbent materials, the partitions inserted in the second, fourth, and sixth slots of the circulation chamber are all sieve plates, or the partitions inserted in the first and seventh slots are all solid plates, and the partitions inserted in the third, fourth, and fifth slots are all sieve plates. In addition, the inlet end of the first three-way valve is connected to the first outlet end, and the inlet end of the second three-way valve is connected to the first outlet end.

[0017] When collecting a protease using an adsorbent material, the partitions inserted in the second and fourth slots of the circulation chamber are screen plates, and the partition inserted in the sixth slot is a solid plate; or the partitions inserted in the first and fifth slots are solid plates, and the partitions inserted in the third and fourth slots are screen plates. The inlet end of the first three-way valve is connected to the second outlet end, and the inlet end of the second three-way valve is connected to the second outlet end.

[0018] Furthermore, it also includes a pre-buffer tank, which is connected to the collection area through a second inlet pipe and is equipped with an eighth electric valve. The pre-buffer tank is connected to the buffer tank through a third inlet pipe and is equipped with a third pump body. The pre-buffer tank is connected to the discharge end through a third drain pipe and is equipped with a ninth electric valve. The pre-buffer tank is equipped with a third liquid level sensor for monitoring the liquid level in the pre-buffer tank.

[0019] Furthermore, it also includes a cleaning main pipe connected to the water source. The cleaning main pipe is equipped with a tenth electric valve and a fourth pump body. It is connected to the four inner cavities of the pre-buffer tank, buffer tank, circulation tank and collection tank through cleaning branch pipes. Each cleaning branch pipe is equipped with an eleventh electric valve and a spray head is located at one end inside the container.

[0020] Furthermore, it also includes several cleaning tanks for storing different cleaning agents. Each cleaning tank is connected to the main cleaning pipe through a liquid injection branch pipe. A fifth pump body and a first check valve are sequentially installed on the liquid injection branch pipe from the cleaning tank to the main cleaning pipe. The first check valve is used to block the flow from the main cleaning pipe to the cleaning tank.

[0021] Furthermore, the first drain pipe, the second drain pipe, and the third drain pipe are all connected to the main cleaning pipe, and the connection point is located on the side of the outlet end of the tenth electric valve. They are then connected to the discharge end through the first discharge branch pipe, and the first discharge branch pipe is equipped with the twelfth electric valve.

[0022] Furthermore, the main cleaning pipe is connected to the collection area through an external cleaning branch pipe, and the external cleaning branch pipe is equipped with a thirteenth electric valve. The collection area is connected to the discharge end through a second discharge branch pipe, and the second discharge branch pipe is equipped with a fourteenth electric valve.

[0023] Furthermore, the first filtration device is a first filter bag fitted on the end of the first collection branch pipe that connects to the collection box, and the second filtration device is a second filter bag fitted on the end of the second collection branch pipe that connects to the collection box.

[0024] A method for collecting crude protease extract, using the above-mentioned system, includes the following steps:

[0025] When collecting two proteases using two adsorbent materials, the following steps are performed: Urine in the buffer tank is injected into the circulation tank through the first inlet pipe; then, one adsorbent material is added to the second inner cavity of the circulation tank through the first automatic feeding device, and another adsorbent material is added to the third inner cavity of the circulation tank through the second automatic feeding device. Simultaneously, the following two steps are performed alternately according to the first preset time: urine in the first inner cavity of the circulation tank is injected into its third and fourth inner cavities through the first pump, and urine in the fourth inner cavity of the circulation tank is injected into its first and second inner cavities through the second pump; finally, the mixture of urine in the second inner cavity of the circulation tank and the corresponding adsorbent material is injected into the collection tank through the first collection branch pipe, and the mixture of urine in the second inner cavity of the circulation tank and the corresponding adsorbent material is injected into the collection tank through the second collection branch pipe. The urine will flow directly into the discharge end, and the two adsorbent materials will remain in the first and second filtration devices, respectively.

[0026] When collecting a protease using an adsorbent material, the following steps are performed: urine in the buffer tank is injected into the circulation tank through the first inlet pipe; then, an adsorbent material is added into the second inner cavity of the circulation tank through the first automatic feeding device. At the same time, the following two steps are performed alternately according to the second preset time: urine in the first inner cavity of the circulation tank is injected into its third inner cavity through the first pump, and urine in the third inner cavity of the circulation tank is injected into its first and second inner cavities through the second pump; finally, the mixture of urine and corresponding adsorbent material in the second inner cavity of the circulation tank is injected into the collection tank through the first collection branch pipe. The urine will flow directly into the discharge end, and the adsorbent material will remain in the first filter device.

[0027] Furthermore, when collecting two proteases using two adsorbent materials, the method further includes the following steps: injecting a mixture of urine and corresponding adsorbent materials from the second inner cavity of the circulation tank into the collection tank through the first collection branch pipe; and after injecting the mixture of urine and corresponding adsorbent materials from the second inner cavity of the circulation tank into the collection tank through the second collection branch pipe, pumping clean water through the fourth pump body to the corresponding nozzles via the cleaning main pipe and the cleaning branch pipes in the first, second, third, and fourth inner cavities of the circulation tank to rinse the adsorbent materials remaining on the screen plate.

[0028] When collecting a protease using an adsorbent material, the method further includes the following steps: after the urine mixture in the second inner cavity of the circulation tank and the corresponding adsorbent material are injected into the collection tank through the first collection branch pipe, clean water is pumped to the corresponding nozzle through the fourth pump body via the cleaning main pipe and the cleaning branch pipes in the first, second and third inner cavities of the circulation tank to rinse the adsorbent material remaining on the screen plate.

[0029] Furthermore, a second check valve is provided between the fourth electric valve and the spray head on the first circulation branch pipe. The second check valve is used to block the flow from the spray head to the fourth electric valve. A third check valve is provided between the fifth electric valve and the spray head on the second circulation branch pipe. The third check valve is used to block the flow from the spray head to the fifth electric valve.

[0030] The beneficial effects of this invention are as follows:

[0031] This invention can simultaneously and automatically collect crude extracts of two proteases, and perform system cleaning during and after production. Moreover, based on the variable multi-chamber structure and alternating circulation mode of the circulation box, it can customize the increase or decrease of the types of proteases collected and control the processing volume accordingly. At the same time, during the protease adsorption process, it can ensure that the adsorbent material is always suspended in the urine, thereby improving the adsorption rate. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the interconnected structure of the crude protease extract collection system of the present invention (with partitions inserted in the second slot, the fourth slot, and the sixth slot);

[0033] Figure 2 This is a schematic diagram of the interconnected structure of the crude protease extract collection system of the present invention (partitions are inserted in the first slot, third slot, fourth slot, fifth slot and seventh slot);

[0034] Figure 3 This is a schematic diagram of the interconnected structure of the crude protease extract collection system of the present invention (partitions are inserted into the first slot, third slot, fourth slot, and fifth slot);

[0035] Figure 4 This is a schematic diagram (top view) of the circulation tank of the present invention;

[0036] Figure 5 This is a schematic diagram of the interconnected structure of the pre-buffer tank of the present invention;

[0037] Figure 6 This is a schematic diagram of the interconnected structure of the buffer tank of the present invention;

[0038] Figure 7 This is a schematic diagram of the interconnected structure of the circulation box of the present invention;

[0039] Figure 8 This is a schematic diagram of the interconnected structure of the collection box of the present invention;

[0040] Figure 9 This is a schematic diagram of the interconnected structure of the cleaning tank of the present invention.

[0041] Labeling Explanation: 1. Pre-buffer tank; 2. Buffer tank; 3. Circulation tank; 3-1. First slot; 3-2. Second slot; 3-3. Third slot; 3-4. Fourth slot; 3-5. Fifth slot; 3-6. Sixth slot; 3-7. Seventh slot; 4. Collection tank; 5. First automatic feeding device; 6. Second automatic feeding device; 7. Cleaning main pipe; 8. Tenth electric valve; 9. Fourth pump body; 10. External cleaning branch pipe; 11. Thirteenth electric valve; 12. Second discharge branch pipe; 13. Fourteenth electric valve; 14. First discharge branch pipe; 15. Twelfth electric valve; 16. Second inlet pipe; 17. Eighth electric valve; 18. Ninth electric valve; 19. Third discharge pipe; 20. Cleaning branch pipe; 21. Eleventh electric valve; 22. 23. Overflow pipe, 24. Third inlet pipe, 25. Third pump body, 26. First drain pipe, 27. First inlet pipe, 28. Second electric valve, 29. Second drain pipe, 30. Third electric valve, 31. Second pump body, 32. First pump body, 33. First three-way valve, 34. Second three-way valve, 35. Second circulation branch pipe, 36. Fifth electric valve, 37. Third check valve, 38. First circulation branch pipe, 39. Fourth electric valve, 40. Second check valve, 41. First collection branch pipe, 42. Sixth electric valve, 43. Second collection branch pipe, 44. Seventh electric valve, 45. First filter bag, 46. Second filter bag, 47. Cleaning tank, 48. Injection branch pipe, 49. Fifth pump body, 50. First check valve. Detailed Implementation

[0042] The invention will now be further described with reference to the accompanying drawings.

[0043] Example 1:

[0044] Please see Figure 1-9 As shown, a crude protease extract collection system includes a buffer tank 2, a circulation tank 3, a collection tank 4, a first pump body 32 and a second pump body 31, a first three-way valve 33 and a second three-way valve 34.

[0045] Buffer tank 2 is used for urine storage, and the urine comes from the collection area. Buffer tank 2 is connected to the discharge end through a first drain pipe 25, and a first electric valve 26 is provided on the first drain pipe 25 to control the opening and closing of the first drain pipe 25. Buffer tank 2 is provided with a first liquid level sensor to monitor the liquid level in buffer tank 2.

[0046] The collection area refers to urinals or other devices that can collect urine, while the discharge end refers to sewage facilities such as sewers and septic tanks.

[0047] Because urine collection scenarios are diverse, it is sometimes impossible for this collection system to keep the buffer tank 2 below the level of each collection zone. Therefore, preferably, the collection system also includes a pre-buffer tank 1. The pre-buffer tank 1 is connected to the collection zone via a second inlet pipe 16, and the second inlet pipe 16 is equipped with an eighth electric valve 17 for controlling the opening and closing of the second inlet pipe 16. The pre-buffer tank 1 is connected to the buffer tank 2 via a third inlet pipe 23, and the third inlet pipe 23 is equipped with a third pump body 24. The pre-buffer tank 1 is connected to the discharge end via a third drain pipe 19, and the third drain pipe 19 is equipped with a ninth electric valve 18 for controlling the opening and closing of the third drain pipe 19. The pre-buffer tank 1 is equipped with a third liquid level sensor for monitoring the liquid level inside the pre-buffer tank 1.

[0048] Specifically, urine from the collection area enters the pre-buffer tank 1 through the second inlet pipe 16. When the liquid level in the pre-buffer tank 1 reaches a preset value, it is then pumped to the buffer tank 2 through the third pump body 24 and the third inlet pipe 23. Generally, the pre-buffer tank 1 is smaller in volume, while the buffer tank 2 is larger in volume.

[0049] The circulation chamber 3 is used for protease adsorption. From left to right, the inner cavity of the circulation chamber 3 is provided with the following slots: first slot 3-1, second slot 3-2, third slot 3-3, fourth slot 3-4, fifth slot 3-5, sixth slot 3-6, and seventh slot 3-7.

[0050] Partitions are inserted into the second slot 3-2, the fourth slot 3-4, and the sixth slot 3-6, sequentially dividing the inner cavity of the circulation box 3 into a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity, as follows: Figure 1 ;

[0051] Alternatively, partitions can be inserted into the first slot 3-1, the third slot 3-3, the fourth slot 3-4, the fifth slot 3-5, and the seventh slot 3-7 to sequentially divide the inner cavity of the circulation box 3 into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, a fourth inner cavity, and a second empty cavity, as shown below. Figure 2 ;

[0052] Alternatively, partitions can be inserted into the first slot 3-1, the third slot 3-3, the fourth slot 3-4, and the fifth slot 3-5 to sequentially divide the inner cavity of the circulation box 3 into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity, as shown below. Figure 3 .

[0053] In this embodiment, partitions are inserted into the second slot 3-2, the fourth slot 3-4, and the sixth slot 3-6, dividing the area inside the circulation box 3 into four equal cavities; partitions are inserted into the first slot 3-1, the third slot 3-3, the fourth slot 3-4, the fifth slot 3-5, and the seventh slot 3-7, dividing the area between the first slot 3-1 and the seventh slot 3-7 inside the circulation box 3 into four equal cavities; partitions are inserted into the first slot 3-1, the third slot 3-3, the fourth slot 3-4, and the fifth slot 3-5, dividing the area between the first slot 3-1 and the fifth slot 3-5 inside the circulation box 3 into three equal cavities.

[0054] The circulation tank 3 is lower than the buffer tank 2, allowing urine in the buffer tank 2 to flow into the circulation tank 3 by gravity. The bottom of the first inner cavity of the circulation tank 3 is connected to the bottom of the buffer tank 2 via a first inlet pipe 27, which is equipped with a second electric valve 28 to control its opening and closing. The bottom of the first inner cavity of the circulation tank 3 is connected to the discharge end via a second drain pipe 29, which is equipped with a third electric valve 30 to control its opening and closing. A first automatic feeding device 5 and a second automatic feeding device 6 are located above the circulation tank 3, used to add different adsorbent materials to the second and third inner cavities of the circulation tank 3, respectively. For example, when the target collected substances are urokinase and ulinastatin, the adsorbent materials are silica gel particles and chitosan, respectively. A second liquid level sensor is installed on the circulation tank 3 to monitor the liquid level within it.

[0055] The first inner cavity of the circulation tank 3 is connected to the pump inlet of the first pump body 32 and the pump outlet of the second pump body 31. The pump outlet of the first pump body 32 and the pump inlet of the second pump body 31 are connected to the inlet end of the first three-way valve 33. The first outlet end of the first three-way valve 33 is connected to the bottom of the fourth inner cavity of the circulation tank 3. The second outlet end of the first three-way valve 33 is connected to the second outlet end of the second three-way valve 34. The inlet end of the second three-way valve 34 is connected to the bottom of the third inner cavity of the circulation tank 3. The circulation tank 3 is provided with a first circulation branch pipe 38 connected to its second inner cavity. A first circulation branch pipe 38 is provided with a spray head at one end in the second inner cavity. The other end of the first circulation branch pipe 38 is connected to the pump outlet of the second pump body 31 and is provided with a fourth electric valve 39 for controlling the opening and closing of the first circulation branch pipe 38. The circulation box 3 is provided with a second circulation branch pipe 35 connected to its third inner cavity. The second circulation branch pipe 35 is provided with a spray head at one end in the third inner cavity. The other end of the second circulation branch pipe 35 is connected to the first outlet end of the first three-way valve 33 and is provided with a fifth electric valve 36 for controlling the opening and closing of the second circulation branch pipe 35.

[0056] Necessarily, filters are provided on both the pump inlet side of the first pump body 32 and the pump inlet side of the second pump body 31.

[0057] Collection box 4 is used for collecting adsorbent material. Collection box 4 is lower than circulation box 3, allowing urine in circulation box 3 to flow into collection box 4 by gravity. Collection box 4 is connected to the bottom of the second inner cavity of circulation box 3 through a first collection branch pipe 41, and a sixth electric valve 42 is provided on the first collection branch pipe 41 to control the opening and closing of the first collection branch pipe 41. Collection box 4 is connected to the first outlet end of the second three-way valve 34 through a second collection branch pipe 43, and a seventh electric valve 44 is provided on the second collection branch pipe 43 to control the opening and closing of the second collection branch pipe 43. Collection box 4 is equipped with a first filter device that separates the urine discharged from the second inner cavity of circulation box 3 into collection box 4 from the corresponding adsorbent material, and a second filter device that separates the urine discharged from the third inner cavity of circulation box 3 into collection box 4 from the corresponding adsorbent material. The bottom of collection box 4 is connected to the discharge end.

[0058] Specifically, the first filtration device is a first filter bag 45 fitted onto the end of the first collecting branch pipe 41 that connects to the collecting box 4, and the second filtration device is a second filter bag 46 fitted onto the end of the second collecting branch pipe 43 that connects to the collecting box 4. After production is completed, the first filter bag 45 and / or the second filter bag 46 can be directly removed.

[0059] In the above technical solution, the pre-buffer tank 1, buffer tank 2, circulation tank 3, and collection tank 4 are all connected to the discharge end through overflow pipe 22 to prevent overflow. Specifically, the connection position of the overflow pipe 22 on the circulation tank 3 should be carefully considered to avoid changes in the baffle plate that would prevent it from functioning properly as an overflow pipe.

[0060] Based on different collection needs, the system is configured as follows:

[0061] When collecting two proteases using two adsorption materials, the partitions inserted in the second slot 3-2, the fourth slot 3-4, and the sixth slot 3-6 in the inner cavity of the circulation chamber 3 are all screen plates to accommodate a larger processing volume, or the partitions inserted in the first slot 3-1 and the seventh slot 3-7 are all solid plates, and the partitions inserted in the third slot 3-3, the fourth slot 3-4, and the fifth slot 3-5 are all screen plates to accommodate a smaller processing volume. In addition, the inlet end of the first three-way valve 33 is connected to the first outlet end, and the inlet end of the second three-way valve 34 is connected to the first outlet end.

[0062] When collecting a protease using an adsorbent material, in the inner cavity of the circulation chamber 3, the partitions inserted in the second slot 3-2 and the fourth slot 3-4 are all screen plates, and the partition inserted in the sixth slot 3-6 is a solid plate to accommodate a larger processing volume. Alternatively, the partitions inserted in the first slot 3-1 and the fifth slot 3-5 are all solid plates, and the partitions inserted in the third slot 3-3 and the fourth slot 3-4 are all screen plates to accommodate a smaller processing volume. The inlet end of the first three-way valve 33 is connected to the second outlet end, and the inlet end of the second three-way valve 34 is connected to the second outlet end.

[0063] Specifically, the method for collecting crude protease extract, using the above system, includes the following steps:

[0064] When collecting two proteases using two adsorbent materials, the following steps are performed: Urine in buffer tank 2 is injected into circulation tank 3 through first inlet pipe 27; then, one adsorbent material is added to the second inner cavity of circulation tank 3 through first automatic feeding device, and another adsorbent material is added to the third inner cavity of circulation tank 3 through second automatic feeding device. At the same time, the following two steps are performed alternately according to a first preset time: urine in the first inner cavity of circulation tank 3 is injected into its third and fourth inner cavities through first pump body 32, and urine in the fourth inner cavity of circulation tank 3 is injected into its first and second inner cavities through second pump body 31; finally, the mixture of urine in the second inner cavity of circulation tank 3 and the corresponding adsorbent material is injected into collection tank 4 through first collection branch pipe 41, and the mixture of urine in the third inner cavity of circulation tank 3 and the corresponding adsorbent material is injected into collection tank 4 through second collection branch pipe 43. The urine will flow directly into the discharge end, and the two adsorbent materials will remain in the first and second filtration devices respectively.

[0065] When collecting a protease using an adsorbent material, the following steps are performed: urine in buffer tank 2 is injected into circulation tank 3 through first inlet pipe 27; then, an adsorbent material is added into the second inner cavity of circulation tank 3 through first automatic feeding device. At the same time, the following two steps are performed alternately according to a second preset time: urine in the first inner cavity of circulation tank 3 is injected into its third inner cavity through first pump body 32, and urine in the third inner cavity of circulation tank 3 is injected into its first and second inner cavities through second pump body 31; finally, the mixture of urine in the second inner cavity of circulation tank 3 and the corresponding adsorbent material is injected into collection tank 4 through first collection branch pipe 41. The urine will flow directly into the discharge end, and the adsorbent material will remain in the first filter device.

[0066] To achieve impact on the bottom adsorbent material, the spray heads installed on the first circulation branch pipe 38 and the second circulation branch pipe 35 should both be located at the bottom of their respective inner cavities.

[0067] In the above-described method for collecting crude protease extract, urine circulates between the various cavities of the circulation tank 3. Since there is a delay in the opening and closing of the fourth electric valve 39 and the fifth electric valve 36, to eliminate the impact of this delay, it is preferable to have a second one-way valve 40 installed between the fourth electric valve 39 and the spray head on the first circulation branch pipe 38. The second one-way valve 40 is used to block the flow from the spray head to the fourth electric valve 39. Similarly, a third one-way valve 37 is installed between the fifth electric valve 36 and the spray head on the second circulation branch pipe 35. The third one-way valve 37 is also used to block the flow from the spray head to the fifth electric valve 36. According to this design, during the circulation of urine between the various cavities of the circulation tank, the fourth electric valve 39 and the fifth electric valve 36 can remain normally open.

[0068] Furthermore, since the first inlet pipe 27, the first outlet end of the first three-way valve 33, the inlet end of the second three-way valve 34, and the pump outlet of the second pump body 31 are all connected to the bottom of the corresponding inner cavity of the circulation tank 3, and the spray heads on the first circulation branch pipe 38 and the second circulation branch pipe 35 are all located at the bottom of their respective inner cavities, the number of air bubbles generated during the circulation of urine between the various inner cavities of the circulation tank 3 will be significantly reduced.

[0069] Example 2:

[0070] Please see Figure 1-9 As shown, based on embodiment 1, it also includes a cleaning main pipe 7 connected to a water source. The cleaning main pipe 7 is equipped with a tenth electric valve 8 and a fourth pump body 9. The tenth electric valve 8 is used to control the opening and closing of the cleaning main pipe 7, and it is connected to the four inner cavities of the pre-buffer tank 1, buffer tank 2, circulation tank 3 and collection tank 4 through cleaning branch pipes 20. Each cleaning branch pipe 20 is equipped with an eleventh electric valve 21, which is used to control the opening and closing of each cleaning branch pipe 20, and each has a spray head located at one end inside the container.

[0071] When the pre-buffer tank 1, buffer tank 2, circulation tank 3 and collection tank 4 need to be cleaned separately, clean water is pumped to the corresponding spray head through the fourth pump body 9 via the main cleaning pipe 7 and the corresponding cleaning branch pipe 20.

[0072] To achieve complete cleaning of the container, the spray heads installed on each cleaning branch pipe 20 should be located at the top of their respective inner cavities.

[0073] When the entire system requires cleaning, including the pre-buffer tank 1, buffer tank 2, circulation tank 3, collection tank 4, and the piping between the containers (including components on the piping), the following steps should be performed:

[0074] Clean water is pumped to the pre-buffer tank 1 through the fourth pump body 9, the main cleaning pipe 7, and the cleaning branch pipe 20 in the pre-buffer tank 1 to clean the pre-buffer tank 1 and the corresponding pipelines.

[0075] The cleaning water in the pre-buffer tank 1 is pumped to the buffer tank 2 through the third pump body 24 and the third inlet pipe 23 to clean the buffer tank 2 and the corresponding pipelines.

[0076] The cleaning water in the buffer tank 2 is injected into the circulation tank 3 through the first inlet pipe 27;

[0077] Inside the circulation tank 3, when collecting two proteases using two adsorbent materials, the following two steps are performed alternately according to a third preset time: the first pump 32 injects the cleaning water in the first inner cavity of the circulation tank 3 into its third and fourth inner cavities, and the second pump 31 injects the cleaning water in the fourth inner cavity of the circulation tank 3 into its first and second inner cavities, cleaning the circulation tank 3 and the corresponding pipelines. After the circulation tank 3 is cleaned, the cleaning water in the circulation tank 3 is injected into the collection tank 4 through the first collection branch pipe 41 and the second collection branch pipe 43, cleaning the collection tank 4 and the corresponding pipelines. The cleaning water in the collection tank 4 flows directly into the discharge end.

[0078] When collecting a protease using an adsorbent material, the following two steps are performed alternately at a fourth preset time: the first pump body 32 injects the cleaning water in the first inner cavity of the circulation tank 3 into its third inner cavity, and the second pump body 31 injects the cleaning water in the third inner cavity of the circulation tank 3 into its first and second inner cavities, cleaning the circulation tank 3 and the corresponding pipelines. After the circulation tank 3 is cleaned, the cleaning water in the circulation tank 3 is injected into the collection tank 4 through the first collection branch pipe 41, cleaning the collection tank 4 and the corresponding pipelines. The cleaning water in the collection tank 4 flows directly into the discharge end.

[0079] The collection area and corresponding pipelines also need to be cleaned. Therefore, the main cleaning pipe 7 is connected to the collection area through the external cleaning branch pipe 10, and the external cleaning branch pipe 10 is equipped with a thirteenth electric valve 11 to control the opening and closing of the external cleaning branch pipe 10. The collection area is connected to the discharge end through the second discharge branch pipe 12, and the second discharge branch pipe 12 is equipped with a fourteenth electric valve 13 to control the opening and closing of the second discharge branch pipe 12.

[0080] Specifically, when the collection area needs to be cleaned separately, clean water is pumped to the corresponding collection area through the fourth pump body 9 via the cleaning main pipe 7 and the external cleaning branch pipe 10. The cleaning water in the collection area flows into the discharge end through the second discharge branch pipe 12.

[0081] The above technical solution also includes several cleaning tanks 47 for storing different cleaning agents. Each cleaning tank 47 is connected to the main cleaning pipe 7 through a liquid injection branch pipe 48. A fifth pump body 49 and a first one-way valve 50 are sequentially provided on the liquid injection branch pipe 48 from the cleaning tank 47 to the main cleaning pipe 7. The first one-way valve 50 is used to block the flow from the main cleaning pipe 7 to the cleaning tank 47.

[0082] Specifically, when the system requires overall deep cleaning, cleaning agent is added to the clean water during the overall cleaning of the system; when the collection area requires separate deep cleaning, cleaning agent is added to the clean water during the separate cleaning of the collection area.

[0083] Necessary is that after the system undergoes a deep cleaning, it needs to be cleaned at least once more. Similarly, after the collection area undergoes a deep cleaning, it needs to be cleaned at least once separately. Generally, the deep cleaning is performed late at night.

[0084] The water source is tap water or a water tank, and the cleaning agent is selected according to the cleaning needs.

[0085] In one embodiment, the first drain pipe 25, the second drain pipe 29, and the third drain pipe 19 are all connected to the main cleaning pipe 7, and the connection position is located on the side of the outlet end of the tenth electric valve 8. They are then connected to the discharge end through the first discharge branch pipe 14, and the first discharge branch pipe 14 is equipped with a twelfth electric valve 15 for controlling the opening and closing of the first discharge branch pipe 14.

[0086] Specifically, according to the above design, when the pre-buffer tank 1 needs to be cleaned separately, clean water can be injected from the bottom to the top through the third drain pipe 19; when the buffer tank 2 needs to be cleaned separately, clean water can be injected from the bottom to the top through the first drain pipe 25; when the circulation tank 3 needs to be cleaned separately, clean water can be injected from the bottom to the top through the second drain pipe 29.

[0087] It should be noted that the above cleaning methods should be selected according to actual production needs.

[0088] As another embodiment, the method for collecting crude protease extract, using the above system, further includes the following steps:

[0089] When collecting two proteases using two adsorbents, the urine mixture in the second inner cavity of the circulation tank 3 and the corresponding adsorbent mixture are injected into the collection tank 4 through the first collection branch pipe 41, and the urine mixture in the second inner cavity of the circulation tank 3 and the corresponding adsorbent mixture are injected into the collection tank 4 through the second collection branch pipe 43. Then, the fourth pump body 9 pumps clean water through the cleaning main pipe 7 and the cleaning branch pipes 20 in the first, second, third and fourth inner cavities of the circulation tank 3 to the corresponding nozzles to spray out, rinsing the adsorbents remaining on the screen plate. The clean water and the remaining adsorbents are then injected into the collection tank 4 through the first collection branch pipe 41 and the second collection branch pipe 43.

[0090] When collecting a protease using an adsorbent material, the urine mixture in the second inner cavity of the circulation tank 3 and the corresponding adsorbent material are injected into the collection tank 4 through the first collection branch pipe 41. Then, clean water is pumped to the corresponding nozzle through the fourth pump body 9 via the cleaning main pipe 7 and the cleaning branch pipes 20 in the first, second, and third inner cavities of the circulation tank 3 to rinse the adsorbent material remaining on the screen plate. The clean water and the remaining adsorbent material are then injected into the collection tank 4 through the first collection branch pipe 41.

[0091] In summary, this invention can simultaneously automate the collection of two crude protease extracts and perform system cleaning during and after production. Furthermore, based on the variable multi-chamber structure and alternating circulation mode of the circulation tank 3, it can customize the increase or decrease of the types of proteases collected and control the processing volume accordingly. At the same time, during the protease adsorption process, it can ensure that the adsorbent material remains suspended in the urine, thereby improving the adsorption rate.

[0092] Of course, the above are only preferred embodiments of the present invention and are not intended to limit the scope of application of the present invention. Therefore, any equivalent changes made to the principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A system for collecting crude protease extract, characterized in that: Includes a buffer tank, a circulation tank, a collection tank, a first pump body and a second pump body, a first three-way valve and a second three-way valve; The buffer tank is used for urine storage. The buffer tank is connected to the discharge end through a first drain pipe, and a first electric valve is provided on the first drain pipe. A first liquid level sensor is provided on the buffer tank to monitor the liquid level inside the buffer tank. The circulation chamber is used for protease adsorption. The inner cavity of the circulation chamber is provided with a first slot, a second slot, a third slot, a fourth slot, a fifth slot, a sixth slot, and a seventh slot from left to right. Specifically: partitions are inserted into the second slot, the fourth slot, and the sixth slot to divide the inner cavity of the circulation chamber into a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity in sequence; or partitions are inserted into the first slot, the third slot, the fourth slot, the fifth slot, and the seventh slot to divide the inner cavity of the circulation chamber into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, a fourth inner cavity, and a second empty cavity in sequence; or partitions are inserted into the first slot, the third slot, the fourth slot, and the fifth slot to divide the inner cavity of the circulation chamber into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity in sequence. The circulation tank is lower than the buffer tank. The bottom of the first inner cavity of the circulation tank is connected to the bottom of the buffer tank through the first inlet pipe, and the first inlet pipe is equipped with a second electric valve. The bottom of the first inner cavity of the circulation tank is connected to the discharge end through the second drain pipe, and the second drain pipe is equipped with a third electric valve. The circulation tank is equipped with a first automatic feeding device and a second automatic feeding device, which are used to feed different adsorbent materials into the second inner cavity and the third inner cavity of the circulation tank, respectively. The circulation tank is equipped with a second liquid level sensor to monitor the liquid level in the circulation tank. The first inner cavity of the circulation box is connected to the pump inlet of the first pump body and the pump outlet of the second pump body. The pump outlet of the first pump body and the pump inlet of the second pump body are connected to the inlet end of the first three-way valve. The first outlet end of the first three-way valve is connected to the bottom of the fourth inner cavity of the circulation box. The second outlet end of the first three-way valve is connected to the second outlet end of the second three-way valve. The inlet end of the second three-way valve is connected to the bottom of the third inner cavity of the circulation box. The circulation box is provided with a first circulation branch pipe connected to its second inner cavity. One end of the first circulation branch pipe located in the second inner cavity is provided with a spray head. The other end of the first circulation branch pipe is connected to the pump outlet of the second pump body and is provided with a fourth electric valve. The circulation box is provided with a second circulation branch pipe connected to its third inner cavity. One end of the second circulation branch pipe located in the third inner cavity is provided with a spray head. The other end of the second circulation branch pipe is connected to the first outlet end of the first three-way valve and is provided with a fifth electric valve. The collection box is used for collecting adsorbent material. The collection box is lower than the circulation box. The collection box is connected to the bottom of the second inner cavity of the circulation box through a first collection branch pipe, and a sixth electric valve is provided on the first collection branch pipe. The collection box is connected to the first outlet end of the second three-way valve through a second collection branch pipe, and a seventh electric valve is provided on the second collection branch pipe. The collection box is equipped with a first filter device that separates the urine discharged from the second inner cavity of the circulation box into the collection box from the corresponding adsorbent material, and a second filter device that separates the urine discharged from the third inner cavity of the circulation box into the collection box from the corresponding adsorbent material. The bottom of the collection box is connected to the discharge end. When collecting two proteases using two adsorbent materials, the partitions inserted in the second, fourth, and sixth slots of the circulation chamber are all sieve plates, or the partitions inserted in the first and seventh slots are all solid plates, and the partitions inserted in the third, fourth, and fifth slots are all sieve plates. In addition, the inlet end of the first three-way valve is connected to the first outlet end, and the inlet end of the second three-way valve is connected to the first outlet end. When collecting a protease using an adsorbent material, the partitions inserted in the second and fourth slots of the circulation chamber are screen plates, and the partition inserted in the sixth slot is a solid plate; or the partitions inserted in the first and fifth slots are solid plates, and the partitions inserted in the third and fourth slots are screen plates. The inlet end of the first three-way valve is connected to the second outlet end, and the inlet end of the second three-way valve is connected to the second outlet end.

2. The protease crude extract collection system according to claim 1, characterized in that: It also includes a pre-buffer tank, which is connected to the collection area through a second inlet pipe and is equipped with an eighth electric valve. The pre-buffer tank is connected to the buffer tank through a third inlet pipe and is equipped with a third pump body. The pre-buffer tank is connected to the discharge end through a third drain pipe and is equipped with a ninth electric valve. The pre-buffer tank is equipped with a third liquid level sensor for monitoring the liquid level in the pre-buffer tank.

3. The protease crude extract collection system according to claim 1, characterized in that: It also includes a cleaning main pipe connected to the water source. The cleaning main pipe is equipped with a tenth electric valve and a fourth pump body. It is connected to the four inner cavities of the pre-buffer tank, buffer tank, circulation tank and collection tank through cleaning branch pipes. Each cleaning branch pipe is equipped with an eleventh electric valve and a spray head is located at one end inside the container.

4. The protease crude extract collection system according to claim 3, characterized in that: It also includes several cleaning tanks for storing different cleaning agents. Each cleaning tank is connected to the main cleaning pipe through a liquid injection branch pipe. A fifth pump body and a first check valve are installed sequentially from the cleaning tank to the main cleaning pipe on the liquid injection branch pipe. The first check valve is used to block the flow from the main cleaning pipe to the cleaning tank.

5. The protease crude extract collection system according to claim 3, characterized in that: The first drain pipe, the second drain pipe, and the third drain pipe are all connected to the main cleaning pipe, and the connection point is located on the side of the outlet end of the tenth electric valve. They are then connected to the discharge end through the first discharge branch pipe, and the first discharge branch pipe is equipped with the twelfth electric valve.

6. The protease crude extract collection system according to claim 3, characterized in that: The main cleaning pipe is connected to the collection area through an external cleaning branch pipe, and the external cleaning branch pipe is equipped with a thirteenth electric valve. The collection area is connected to the discharge end through a second discharge branch pipe, and the second discharge branch pipe is equipped with a fourteenth electric valve.

7. The protease crude extract collection system according to claim 1, characterized in that: The first filtration device is a first filter bag fitted on the end of the first collection branch pipe that connects to the collection box, and the second filtration device is a second filter bag fitted on the end of the second collection branch pipe that connects to the collection box.

8. A method for collecting crude protease extract, using the crude protease extract collection system according to any one of claims 1-7, characterized in that: Includes the following steps: When collecting two proteases using two adsorbent materials, the following steps are performed: Urine in the buffer tank is injected into the circulation tank through the first inlet pipe; then, one adsorbent material is added to the second inner cavity of the circulation tank through the first automatic feeding device, and another adsorbent material is added to the third inner cavity of the circulation tank through the second automatic feeding device. Simultaneously, the following two steps are performed alternately according to the first preset time: urine in the first inner cavity of the circulation tank is injected into its third and fourth inner cavities through the first pump, and urine in the fourth inner cavity of the circulation tank is injected into its first and second inner cavities through the second pump; finally, the mixture of urine in the second inner cavity of the circulation tank and the corresponding adsorbent material is injected into the collection tank through the first collection branch pipe, and the mixture of urine in the second inner cavity of the circulation tank and the corresponding adsorbent material is injected into the collection tank through the second collection branch pipe. The urine will flow directly into the discharge end, and the two adsorbent materials will remain in the first and second filtration devices, respectively. When collecting a protease using an adsorbent material, the following steps are performed: urine in the buffer tank is injected into the circulation tank through the first inlet pipe; then, an adsorbent material is added into the second inner cavity of the circulation tank through the first automatic feeding device. At the same time, the following two steps are performed alternately according to the second preset time: urine in the first inner cavity of the circulation tank is injected into its third inner cavity through the first pump, and urine in the third inner cavity of the circulation tank is injected into its first and second inner cavities through the second pump; finally, the mixture of urine and corresponding adsorbent material in the second inner cavity of the circulation tank is injected into the collection tank through the first collection branch pipe. The urine will flow directly into the discharge end, and the adsorbent material will remain in the first filter device.

9. A method for collecting crude protease extract according to claim 8, characterized in that: When collecting two proteases using two adsorbent materials, the process also includes the following steps: injecting a mixture of urine and corresponding adsorbent materials from the second inner chamber of the circulation tank into the collection tank through the first collection branch pipe; and after injecting the mixture of urine and corresponding adsorbent materials from the second inner chamber of the circulation tank into the collection tank through the second collection branch pipe, pumping clean water through the fourth pump body to the corresponding nozzles via the cleaning main pipe and the cleaning branch pipes in the first, second, third, and fourth inner chambers of the circulation tank to rinse the adsorbent materials remaining on the screen plate. When collecting a protease using an adsorbent material, the method further includes the following steps: after the urine mixture in the second inner cavity of the circulation tank and the corresponding adsorbent material are injected into the collection tank through the first collection branch pipe, clean water is pumped to the corresponding nozzle through the fourth pump body via the cleaning main pipe and the cleaning branch pipes in the first, second and third inner cavities of the circulation tank to rinse the adsorbent material remaining on the screen plate.

10. A method for collecting crude protease extract according to claim 8, characterized in that: A second check valve is provided between the fourth electric valve and the spray head on the first circulation branch pipe. The second check valve is used to block the flow from the spray head to the fourth electric valve. A third check valve is provided between the fifth electric valve and the spray head on the second circulation branch pipe. The third check valve is used to block the flow from the spray head to the fifth electric valve.

Images