Solid waste resource utilization method based on microorganism combined with full bionic simulation

Abstract

The present invention relates to a fluorescent Pseudomonas N1 (Pseudomonas fluorescens), and a method for utilizing solid waste resources in microorganism combined with full bionic simulation, belonging to the technical field of biological fermentation and soil remediation. The light, temperature, gas and heat conditions of organic carbon cyclic transformation in the soil are simulated, solid waste is mixed and piled in the soil or between hills, and basic mixing is performed according to the carbon-nitrogen ratio, acidity and alkalinity, and water content of the solid waste. According to the principle of anaerobic fermentation, the pile is not turned over. During the composting process, a bionic cover is used to introduce high-carbon, high-salt, alkaline or acidic waste liquid, and the anaerobic fermentation process is controlled to stop at the acid production stage, so that the generated organic acid is fully mixed with the solid waste, and the acidic substance is used to release the nutrients in the solid waste, thereby becoming the nutrient elements chelated by the acidic substance, and finally achieving the purpose of resource utilization of solid waste.

Description

Technical Field

[0001] The present invention relates to a fluorescent Pseudomonas N1 and its application in the resource utilization of solid waste combined with full bionic simulation, belonging to the technical field of biological fermentation and soil pollution remediation. This application is a divisional application, and the parent application number is CN202210116969.X. Background Art

[0002] Solid waste contains a large amount of nutrients, but due to silicate capping and alkaline conditions, most of them cannot be directly absorbed and utilized by crops. The traditional composting and fermentation process is costly, inefficient, and lacks large-scale consumption capacity, and cannot be widely promoted and used.

[0003] Various nutrients in the soil form a natural circulation system. Especially under specific environmental conditions, such as swamps and ocean tidal flats, a large number of carbon mines rich in nutrients that can be absorbed by plants will appear, such as peat mines and turf moss.

[0004] Solid waste from industry and agriculture has gradually become a global problem. Most world and national standards use landfill treatment, which may not only cause toxic substances to leak, but also leave hidden dangers such as landslides and soil and water conservation in the future. A method that can be used in large quantities is urgently needed for treatment. If these solid wastes can be matrixed, the large cycle of industry, mining and agriculture will be realized, and the toxic and harmful substances in them can be phytoremediated by using the growth characteristics of plants. Summary of the invention

[0005] In view of the above background, the present invention has developed a method for simulating bionic swamp covers and large-scale solid waste matrixing. The bionic covers are used to control the light, temperature, gas and heat conditions in the solid waste mixture composting process, and the organic carbon formation process in the bionic environment; the carbon-nitrogen ratio, pH change, salinity change and moisture content inside the mixture are measured by probes, and the acid production fermentation conditions of the mixture are adjusted by injecting waste liquid using the released covers; the acid production link is determined by silicate analysis, acid compound secretion, and soluble nutrient values ​​inside the mixture. After completion, it is taken out for cultivation of various crops.

[0006] Specifically, the present invention relates to a method for stripping the silicate surface layer in solid waste and activating various nutrients by utilizing bionic simulation of the natural generation process of organic carbon ore, combining biological fermentation acid production conditions, utilizing the carbon-nitrogen ratio, pH value and salinity in the waste liquid to regulate the acid production.

[0007] The present invention relates to a method for resource utilization of solid wastes by combining microorganisms with full bionic simulation, wherein the microorganism used is Pseudomonas fluorescens N1. The method comprises the following steps:

[0008] (1) Coal gangue with a particle size of 2-5 mm is piled at the bottom, with a thickness of 5-10 m;

[0009] (2) stacking a bionic swamp mixture on the first layer of coal gangue, specifically a mixture of sludge, kitchen waste and fly ash, adjusting the water content of the mixture so that the fermentation water meets the basic requirements of anaerobic fermentation; at the same time, controlling the water content of the mixture to 40%-60%, adjusting the carbon-nitrogen ratio to (25-35): 1, the pH value to 4.6-6.0, the salinity to 20%-30%, the stacking thickness to 2-5m, and performing anaerobic fermentation;

[0010] (3) alternately repeating steps (1)-(2), performing multi-layer stacking according to the stacking processing volume, thereby improving the stacking efficiency and the total processing volume;

[0011] (4) On the top of the stacking layer, a bionic swamp cover is laid, wherein the bionic swamp cover is composed of solid waste. During the anaerobic fermentation process, the bionic swamp cover is used to transport functional microbial eluent, carbon-nitrogen ratio eluent, pH adjustment eluent and high-salt eluent to the stacking layer below. At the same time, the bionic swamp cover is used to adjust the wet heat energy storage of the bottom stacking layer. A sunlight film is set on the bionic swamp to concentrate energy;

[0012] (5) A sensing probe is arranged at the bottom of the bionic swamp cover to timely feedback the temperature and humidity of the fermentation pile. When the temperature is too high or too low, the above-mentioned eluent at a certain temperature is delivered to control it.

[0013] Furthermore, the coal gangue in step (1) can be replaced by coal gangue and magnesium slag; and the fly ash in step (2) can be replaced by desulfurized ash.

[0014] Furthermore, the bionic swamp mixture of step (2) is kitchen waste: sludge: fly ash (or desulfurization ash) in a volume ratio of 10:5:3; the carbon-nitrogen ratio is adjusted to 35:1;.

[0015] Furthermore, the bionic swamp cover of step (4) is composed of straw, mushroom residue, and garden waste; preferably straw and mushroom residue.

[0016] Furthermore, the temperature of step (5) is maintained at 16° C. to 35° C.; and the humidity is maintained at 40% to 60%.

[0017] Furthermore, in the microbial washing solution of step (4), the microorganism is an acid-producing anaerobic fermentation microorganism, preferably Pseudomonas fluorescens N1, and the daily spraying water volume is 1.00 m 3 / m 3Soil, lasting 50-60 days; the carbon-nitrogen ratio eluent is a conventional solution with a carbon-nitrogen ratio of 35:1. The rich carbon-nitrogen ratio ensures that the anaerobic fermentation stays in the acid-producing stage; the pH eluent is added according to the feedback from the probe. When the pH is less than 4.6, alkaline waste liquid is added, and when the pH is greater than 6.0, acidic waste liquid is added.

[0018] The present invention also relates to a method for preparing a cultivation medium by modifying solid waste with microorganisms in cooperation with coal gangue, comprising the following steps:

[0019] (1) adding coal gangue to magnesium slag, mixing them thoroughly, sieving them, and adding them in an amount of 10-15% of the volume of the magnesium slag. The particle size of the mixed material is controlled at 2-5 mm, and the material is piled for 5-10 days;

[0020] (2) adding vinegar dregs in an amount of 20-30% of the volume of the magnesium slag, mixing well, and adjusting the pH value to 5.5-6.5 using wood vinegar;

[0021] (3) adding phosphate rock powder in an amount of 5-10% of the total volume of the above materials and mixing well;

[0022] (4) Spray phosphate-solubilizing bacteria + Xanthomonas on the surface of the material and adjust the carbon-nitrogen ratio to about 25:1. The daily spraying water volume is 0.50-1.00m 3 / m 3 Soil for 30-40 days, allowing the phosphate-solubilizing bacteria to continuously release organic acids and form a cultivation matrix.

[0023] Furthermore, the phosphate-solubilizing bacteria is preferably Pseudomonas fluorescens, and the Xanthomonas campestris is preferably Xanthomonas campestris.

[0024] Furthermore, the phosphate-solubilizing bacteria is preferably Pseudomonas fluorescens N1, which has a registration number of CGMCC No. 23192 in the General Microbiological Center of China National Microbiological Culture Collection Administration.

[0025] The present invention uses functional microorganisms to process and mix solid waste according to its acidity and alkalinity, specific nutrition, hydrophilicity, particle size, microecological adaptability and other characteristics, and places it in a seedling pot with an impermeable membrane at the bottom for cultivation, which adapts to the rooting and rapid growth of plants while being completely isolated from the surrounding soil until the solid waste matrix is ​​wrapped by the plant root system, and the content of various pollutants in the solid waste matrix is ​​detected to reach the national standard. The rooted plants are transplanted in seedling pots. After transplanting, the bottom is opened according to different greening requirements, so that the main root system penetrates into the corresponding plot, thereby fixing the plant and facilitating its upward extension; the fibrous root system wraps the solid waste matrix, absorbs and passivates the harmful substances in the matrix, and the plant with matrix formed in this way can be moved at will, and the survival rate is high, and it can also be used in the local plot to form a landscape. Various types of solid waste will return to the land and nature through plant growth, achieving large-scale reduction of solid waste and the purpose of using plants to repair various pollutants.

[0026] The present invention uses functional microorganisms as main tools, and solid waste raw materials are modified and treated. Based on the accurate grasp of functional microorganisms, and continuous testing, the modified matrix raw materials can be flexibly matched to meet different plant periods and different growth characteristics, similar to the principle of soil testing and formula fertilization. The matrix raw materials obtained in this way, the matrix of the configuration will greatly promote plant rooting and growth, with good sustainability, and provide a matrix platform for plant restoration, and good plant growth can bring better repair effects. At the same time, the plants produced by this technology are produced with nutrient pots, do not occupy cultivated land, do not carry out topsoil excavation, destroy the original ecology of the nursery, both can be carried out ecological restoration by filling untreated solid waste around the nutrient pots with row spacing, and the matrix wrapped by the root system can also be directly sold, becoming a higher added value, a high survival rate with soil ball plants, for road greening and mine ecological restoration.

[0027] Beneficial effects of this application:

[0028] 1. This application simulates the laws of natural carbon cycle, sets up a bionic swamp cover, and piles up solid waste; during the stacking process, sets up anaerobic fermentation light, temperature, gas and heat conditions, adjusts the fermentation direction by externally connecting solid waste liquid, stays in the acid production stage, and strictly controls the transition to the small molecule gas link of methane and carbon dioxide; acidic organic matter gradually penetrates into the solid waste, removes the blockage of silicates to nutrients, activates various nutrients, and converts solid waste into high-quality arable soil.

[0029] 2. We have studied for the first time the method of converting solid waste into plant cultivation matrix through biological modification. Specifically, magnesium slag and coal gangue are crushed into a certain particle size. After modification by phosphate-solubilizing microorganisms, their adsorption capacity is significantly improved. The modified coal gangue can adsorb phosphates in solid waste, and then release high concentrations of effective phosphorus that can be directly used by plants after biodegradation by phosphate-solubilizing microorganisms. Phosphorus-solubilizing microorganisms in modified coal gangue and its coordination with other solid wastes such as magnesium slag to repair contaminated soil and promote plant development is the first proposal of this application. We found that a large number of trace elements in magnesium slag that can be absorbed and utilized by plants are concentrated in insoluble silicates. By mixing with coal gangue and using microbial modification methods, the insoluble salts in magnesium slag can be adsorbed by the modified coal gangue, and then degraded into soluble ions (K+, Na+, effective nitrogen, phosphorus, potassium, etc.) by phosphate-solubilizing bacteria, and then directly absorbed and utilized by plants.

[0030] 3. The organic acids produced by phosphate-solubilizing bacteria during their growth and reproduction can not only reduce the pH value in the soil environment, but also directly release the phosphorus in fixed phosphates into the soil. We found that a large number of trace elements in magnesium slag that can be absorbed and utilized by plants are concentrated in insoluble silicates. By mixing with coal gangue and modifying it with microbial phosphate-solubilizing bacteria, the insoluble salts in magnesium slag can be adsorbed by the modified coal gangue, and then degraded into soluble ions (K+, Na+, effective nitrogen, phosphorus, potassium, etc.) by phosphate-solubilizing bacteria, and then directly absorbed and utilized by plants. Experiments have shown that phosphate-solubilizing bacteria can cooperate with coal gangue to modify solid waste, thereby releasing phosphorus in fixed phosphates into the soil for use by plants.

[0031] 4. Organic acids such as acetic acid, lactic acid, malic acid, oxalic acid, succinic acid, citric acid and gluconic acid produced by phosphate-solubilizing bacteria during growth and reproduction can not only reduce the pH value in the soil environment, directly release phosphorus in fixed phosphate into the soil, but also form chelates with iron and aluminum ions for absorption and utilization by crops. The organic acids produced by the metabolism of phosphate-solubilizing bacteria can block the adsorption sites of soil phosphorus, or improve the effectiveness of phosphorus by forming complexes with cations on the surface of soil minerals. Xanthomonas can produce a large amount of xanthan gum. The present invention first discovered that it can promote the release of effective phosphorus in solid wastes and produce organic acids when used in conjunction with phosphate-solubilizing bacteria. The effect of synergistically modifying magnesium slag solid waste with coal gangue is better, and the improved solid waste is made into plant growth matrix, which can promote plant growth.

[0032] 5. The content of different coal gangues affects the effective phosphorus content of the matrix and the production of organic acids. Compared with the control without adding coal gangue, the effective phosphorus content of the matrix of other experimental examples is higher. Among them, the effect is best when the coal gangue content is 10-15 parts by volume. The adsorption capacity of coal gangue after microbial modification is extremely strong, and the phosphorus in the fixed phosphate is directly released. Oxalic acid is the most representative type of organic acid and one of the important organic acids required for plant growth. Through the synergistic modification of solid waste magnesium slag and coal gangue by Fluoromonas and Xanthomonas, the organic acids in the solid waste are released, thereby promoting plant growth. Compared with other microorganisms, its effect of solubilizing phosphorus and releasing organic acids is not as good as the combined use of Fluoromonas + Xanthomonas of this application.

[0033] 6. This application is the first to ferment solid waste with full bionic simulation of swamp reaction, and obtain the optimized parameters that affect the acid production reaction of solid waste. By continuously optimizing the parameters, it is found that the water content, salinity, and carbon-nitrogen ratio in the full bionic simulation of swamp reaction are very important. The fermentation decomposition effect is the highest when the carbon-nitrogen ratio is adjusted to 35:1, the salinity is 20%, and the water content is 60%. This shows that the effect of dephosphorization and promoting organic acid production in the acidic solid waste matrix environment is good. The effective phosphorus content and acid production in the process are both high. At the same time, the parameters can maintain the acidic environment and promote the persistent decomposition of microorganisms.

[0034] 7. Simulate the light, temperature, gas and heat conditions of the circulation and transformation of organic carbon in the soil, mix the solid waste and pile it in the soil or between hills, and basically mix it according to the carbon-nitrogen ratio, acidity, alkalinity and water content of the solid waste. According to the principle of anaerobic fermentation, do not turn the pile over. During the composting process, use a bionic cover to introduce high-carbon, high-salt, alkaline or acidic waste liquid, regulate the amount of oxygen, and control the anaerobic fermentation process to stop at the acid production stage, so that the produced organic acid and solid waste are fully mixed, and the acidic substances are used to release the nutrients in the solid waste, which become the nutrients chelated by the acidic substances, ultimately achieving the purpose of resource utilization of solid waste.

[0035] Collection Instructions

[0036] Bacteria name: Pseudomonas fluorescens

[0037] Latin name: Pseudomonasfluorescens

[0038] Strain number: N1

[0039] Depository: China National Microbiological Culture Collection Administration General Microbiology Center

[0040] Abbreviation of depository institution: CGMCC

[0041] Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing

[0042] Date of deposit: August 25, 2021

[0043] CGMCC Registration Number: CGMCC No.23192 BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 On-site photo of the bionic simulated fermentation test field

[0045] Figure 2 Mixture diagram of bionic bog mix

[0046] Figure 3 Bionic simulation fermentation test layout diagram DETAILED DESCRIPTION

[0047] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] The technical principle of this application is: the pre-treated solid waste matrix raw materials are proportioned according to the modification results, following the basic nutrient principles, pH, salinity, hydrophilicity, carbon-nitrogen ratio, accessing functional bacteria, regulating the root growth microecology, using seedling pots to hold the matrix, planting plants, and densely stacking the seedling pots, appropriately adding untreated solid waste matrix for gap filling, constructing a micro-moisture conservation environment, and utilizing the maintenance of the plant root microenvironment. After new branches sprout, the plants are transplanted with seedling pots. According to the purpose of transplanting, the bottom of the seedling pot is treated according to the aperture. For conventional greening, the aperture is less than 5 cm to fix the plants; for soil and water conservation greening, the aperture is increased to more than 15 cm to release more roots. Most of the roots remain inside the solid waste matrix to adsorb and passivate various types of polluting toxic and harmful substances for plant restoration.

[0049] The Xanthomonas of the present invention is Xanthomonas campestris NRRLB-1459, but is not limited to this species, and all Xanthomonas can achieve the present invention; the Pseudomonas of the present invention is Pseudomonas fluorescens N1, but is not limited to this species, and all Pseudomonas can achieve the present invention. The above strains are adjusted to a bacterial solution concentration of about 109 cells per milliliter (OD600=1.0).

[0050] Example 1: A method for resource utilization of solid waste by combining microorganisms with full bionic simulation, comprising the following steps:

[0051] (1) Coal gangue with a particle size of 3 mm is piled at the bottom, with a thickness of 6 m;

[0052] (2) stacking a bionic swamp mixture on the first layer of coal gangue, specifically a mixture of sludge, kitchen waste and fly ash, adjusting the water content of the mixture to 60% so that the fermentation water meets the basic requirements of anaerobic fermentation; at the same time, adjusting the carbon-nitrogen ratio of the mixture to 35:1, the pH value to 5.5, the salinity to 20%, the stacking thickness to 3m, and anaerobic fermentation;

[0053] (3) alternately repeating steps (1)-(2), performing multi-layer stacking according to the stacking processing volume, thereby improving the stacking efficiency and the total processing volume;

[0054] (4) On the top of the stacking layer, a bionic swamp cover is laid. The bionic swamp cover is composed of crushed straw and mushroom residue mixed in a volume ratio of 3:2, and the crushed particle size of the two components is 3 cm; during the anaerobic fermentation process, the bionic swamp cover is used to transport functional microbial eluent, carbon-nitrogen ratio eluent, pH adjustment eluent and high-salt eluent to the stacking layer below, and at the same time, the bionic swamp cover is used to adjust the wet heat energy storage of the bottom stacking layer, and a sunlight film is set on the bionic swamp to concentrate energy; the microorganism in the microbial eluent is an acid-producing anaerobic fermentation microorganism, preferably Pseudomonas fluorescens N1 (Pseudomonasfluorescens); the daily spraying water volume is 1.00m 3 / m 3 soil, for 60 days;

[0055] (5) A sensing probe is arranged at the bottom of the bionic swamp cover to timely feedback the temperature and humidity of the fermentation pile. When the temperature is too high or too low, the above-mentioned eluent is delivered at a certain temperature to control the temperature; the temperature is maintained at 30°C; and the humidity is maintained at 60%.

[0056] Example 2: A method for resource utilization of solid waste by combining microorganisms with full bionic simulation, comprising the following steps:

[0057] (1) Coal gangue and magnesium slag with a particle size of 3 mm are piled at the bottom, with the amount of coal gangue added being 10% of the volume of the magnesium slag, and then vinegar dregs are added, with the amount added being 30% of the volume of the magnesium slag; they are mixed evenly and piled to a thickness of 6 m;

[0058] (2) stacking a mixture of sludge, kitchen waste and fly ash on the first layer, adjusting the water content of the mixture to 60% so that the fermentation water content meets the basic requirements of anaerobic fermentation; at the same time, adjusting the carbon-nitrogen ratio of the mixture to 35:1, the pH value to 5.5, the salinity to 20%, the stacking thickness to 3m, and anaerobic fermentation;

[0059] (3) alternately repeating steps (1)-(2), performing multi-layer stacking according to the stacking processing volume, thereby improving the stacking efficiency and the total processing volume;

[0060] (4) On the top of the stacking layer, a bionic swamp cover is laid. The bionic swamp cover is composed of straw and mushroom residue mixed in a volume ratio of 3:2, and the crushed particle size of the two components is 3 cm. During the anaerobic fermentation process, the bionic swamp cover is used to transport functional microbial eluent, carbon-nitrogen ratio eluent, pH adjustment eluent and high-salt eluent to the stacking layer below. At the same time, the bionic swamp cover is used to adjust the wet heat storage energy of the bottom stacking layer. A sunlight film is set on the bionic swamp to concentrate energy. In the microbial eluent, the microorganism is an acid-producing anaerobic fermentation microorganism, preferably a mixed bacteria of Pseudomonas fluorescens and Xanthomonas campestris (mixed in equal solution volumes), and the daily spraying water volume is 1.00m 3 / m 3 soil, for 50 days;

[0061] (5) A sensing probe is arranged at the bottom of the bionic swamp cover to timely feedback the temperature and humidity of the fermentation pile. When the temperature is too high or too low, the above-mentioned eluent is delivered at a certain temperature to control the temperature; the temperature is maintained at 30°C; and the humidity is maintained at 60%.

[0062] Example 3 A method for modifying solid waste by using microorganisms in collaboration with coal gangue materials comprises the following steps:

[0063] (1) Magnesium slag and coal gangue are added and mixed thoroughly, sieved, and the added amount is 10% of the volume of the magnesium slag. The particle size of the mixed material is controlled at 2 mm and stacked for 8 days;

[0064] (2) adding vinegar dregs in an amount of 30% of the volume of the magnesium slag, mixing well, and adjusting the pH value to 5.5 using wood vinegar;

[0065] (3) Spray Pseudomonas fluorescens N1 + Xanthomonas campestris (mixed by equal solution volumes) on the surface of the material, with a daily spraying volume of 1.00 m 3 / m 3 Soil, lasting 35 days.

[0066] Example 4: A method for preparing a cultivation medium by modifying solid waste with microorganisms and coal gangue, comprising the following steps:

[0067] (1) Magnesium slag and coal gangue are added and mixed thoroughly, sieved, and the added amount is 10% of the volume of the magnesium slag. The particle size of the mixed material is controlled at 3 mm and stacked for 5 days;

[0068] (2) adding vinegar dregs in an amount of 30% of the volume of the magnesium slag, mixing, and adjusting the pH value to 5.5 using wood vinegar; wood vinegar: pH value 3.3, containing 53.0% of the total amount of amino acids;

[0069] (3) adding phosphate rock powder in an amount of 8% of the total volume of the above materials and mixing well;

[0070] (4) Spray phosphate-solubilizing bacteria and Xanthomonas on the surface of the material, and adjust the carbon-nitrogen ratio to about 25:1. The daily spraying water volume is 1.00m 3 / m 3 Soil for 30 days, so that the phosphate-solubilizing bacteria can continuously release organic acids and form a cultivation medium;

[0071] Further options include:

[0072] (5) placing the above-mentioned substrate into seedling pots, transplanting the plants, and leaving no gaps between the seedling pots or filling them with soil to form a micro-moisture conservation environment to promote rapid root growth;

[0073] (6) When new buds sprout or new leaves unfold, sell the plants or restore the ecology in situ.

[0074] The phosphate-solubilizing bacteria is preferably Pseudomonas fluorescens,

[0075] The Xanthomonas campestris is preferably Xanthomonas campestris NRRLB-1459,

[0076] In the step (4), when adjusting the carbon-nitrogen ratio, ammonia water and nitrogen fertilizer are selected as the nitrogen source, and straw agricultural waste is selected as the carbon source.

[0077] The step (4) also includes calculating and adding corresponding nitrogen fertilizer (urea, KNO) to the substrate with reference to the main nutrient content of the cultivation substrate. 3 、NH4H2PO 4 etc.), phosphate fertilizer (superphosphate (solidified phosphorus) + potassium dihydrogen phosphate), potash fertilizer (KNO 3 、KH2PO 4 wait).

[0078] Experiment 1: Experiment on the modification of solid waste by microorganisms and coal gangue materials as substrate

[0079] Experimental method: The organic acid produced by microorganisms during their growth and reproduction can not only reduce the pH value in the soil environment, but also directly release phosphorus in fixed phosphates into the soil. This experiment mainly studies the process parameters of microorganisms cooperating with coal gangue to modify solid waste magnesium slag. The specific method is shown in Example 3. By comparing the proportions of magnesium slag, coal gangue, and vinegar dregs with different ratios, as well as the phosphate-solubilizing bacteria and Xanthomonas species used to modify solid waste, a matrix for plant growth and cultivation is formed. The moisture content (%) of the matrix, the aeration capacity (maximum volumetric oxygen content per square meter of the matrix, unit m 3 / m 2 ), available phosphorus content (mg / kg) and organic acid (oxalic acid) content (mg / kg). Each treatment was repeated three times and the average value was taken.

[0080] Table 1 Solid waste modification test table

[0081]

[0082]

[0083] Table 2 Effects of different modification conditions on solid waste modification matrix indicators

[0084]

[0085]

[0086] Test results:

[0087] The content of different gangues affects the effective phosphorus content of the matrix and the production of organic acids. Compared with the control 4 without adding gangue, the effective phosphorus content of the matrix of other experimental examples is higher. Among them, the effect is best when the gangue content is 10-15 parts by volume. The adsorption capacity of gangue after microbial modification is extremely strong, and phosphorus in fixed phosphate is directly released. The content of organic acid (oxalic acid) is also the highest in Experimental Example 8. Oxalic acid is the most representative type of organic acid and one of the important organic acids required for plant growth. Solid waste magnesium slag and gangue are modified by fluorescein and yellow monosporus to release organic acids in solid waste, thereby promoting plant growth. In addition, compared with other microorganisms, its effect of dephosphorylating and releasing organic acids is not as good as the combined use of fluorescein + yellow monosporus of the present application, and the comprehensive physical and chemical properties of the improved solid waste transformation matrix are better than the use of one of the microorganisms alone, such as controls 1 and 2.

[0088] Different pH values ​​have different effects on the phosphorus solubilization effect of the strain. After 30 days of modified solid waste, the effective phosphorus content is compared with the control 5-6. The phosphorus solubilization and organic acid production effects at pH 5.0 and 6.0 are weaker, and a higher amount of phosphorus solubilization can be produced at pH 5.5. The effective phosphorus content and organic acid content of Experimental Example 8 are the highest at pH 5.5. This shows that the effect of phosphorus solubilization and promoting organic acid production in the acidic solid waste matrix environment is good.

[0089] This application uses magnesium slag as a hydrophobic raw material and vinegar dregs as a hydrophilic raw material for the first time. Through the reasonable ratio of the two materials and the adjustment of particle size, the moisture content and ventilation of the substrate suitable for plant cultivation are achieved. Through comparative tests, it can be seen that when the volume ratio of magnesium slag to vinegar dregs is 100:30, the moisture content and ventilation of the substrate are optimal. Compared with magnesium slag solid waste without coal gangue, the ventilation of the substrate is significantly reduced, and compared with magnesium slag solid waste without vinegar dregs, the moisture content of the substrate is also significantly reduced.

[0090] Experiment 2: Orthogonal design analysis of factors affecting the repair capacity of solid waste transformation matrix

[0091] Experimental method: To find the factors and optimal combination that affect the ability of solid waste transformation matrix to repair heavy metal contaminated soil and the effect of phosphorus solubilization of available phosphorus, and to provide a theoretical basis for the optimal combination of restoration technology after solid waste transformation. The particle size A (2 mm for level 1, 3 mm for level 2, and 5 mm for level 3), the amount of coal gangue added B (percentage of magnesium slag volume) (10% for level 1, 13% for level 2, and 15% for level 3), the amount of phosphate solubilizing bacteria C (0.50 m3 for level 1) and the amount of phosphate solubilizing bacteria C (0.50 m3 for level 1) were used to determine the optimal combination of restoration technology after solid waste transformation. 3 / m 3 , level 2 is 0.80m 3 / m 3 , level 3 is 1.00m 3 / m 3 ) three conditions as variables, among which the phosphate-solubilizing bacteria was Pseudomonas fluorescens N1, and the removal rate of heavy metal lead and the effective phosphorus content were used as indicators to select the optimal combination, L9(3 3 ) orthogonal table. The lead removal rate is specifically calculated by simulating the preparation of lead-contaminated soil with a moisture content of 30%. 3 ) 2 The solution was added to the matrix soil, and the Pb content in the soil was controlled to 200 mg / L. After mixing evenly, it was placed in a constant temperature incubator for 90 days. The soil was taken out every 10 days, and deionized water was added to the contaminated soil. The relative moisture content of the soil was controlled to be about 30% by mass method. The materials at the above test levels were added to simulate remediation for 30 days, and the Pb content was determined. 2+ Concentration and effective phosphorus content. Removal rate of heavy metal lead = (C0-C1) / C0, where C0 is the lead concentration of the initial solution before the test (mg / L); C1 is the lead concentration of the residual solution after adsorption (mg / L). Each treatment was repeated three times and the average value was taken.

[0092] Table 3 Orthogonal design table affecting the repair capacity of solid waste transformation matrix

[0093]

[0094]

[0095] Test results: As can be seen from the table above, through research we have concluded that the factors affecting the repair capacity of solid waste transformation matrix are mainly material particle size A, coal gangue addition amount B, and phosphate solubilizing bacteria dosage C. The optimal solution affecting the repair capacity of solid waste transformation matrix for heavy metal lead pollution is A3B3C3, that is, the mixed matrix is ​​crushed to 5mm particle size, the coal gangue addition amount is 15%, and the phosphate solubilizing bacteria dosage is 1.00m 3 / m 3The optimal solution for the phosphorus solubilization effect of solid waste transformation matrix is ​​A1 B2C3, that is, the mixed matrix is ​​crushed to a particle size of 2 mm, the amount of coal gangue added is 13%, and the amount of phosphorus solubilizing bacteria is 1.00 m 3 / m 3 .

[0096] The order of influence of each factor is the ability to repair heavy metal lead pollution: B (coal gangue addition) > A (crushed particle size) > C (phosphorus solubilizing bacteria dosage). The phosphorus solubilizing effect of solid waste transformation matrix: A (crushed particle size) > C (phosphorus solubilizing bacteria dosage) > B (coal gangue addition).

[0097] Experiment 3: Optimization of solid waste combination based on full bionic simulation

[0098] Test method: The fully biomimetic simulation of the solid waste combination of the present invention, in conjunction with the production of organic acids by microorganisms during the growth and reproduction process, can not only reduce the pH value in the soil environment, but also directly release phosphorus in fixed phosphates into the soil. This test mainly studies the optimization of process parameter conditions for the fully biomimetic simulation of the solid waste combination. The specific method is shown in Example 1. By comparing different ratios of modified solid waste, a matrix for plant growth and cultivation is formed. The effective phosphorus content (mg / kg) and organic acid (oxalic acid) content (mg / kg) of the bottom solid waste layer are determined after 60 days of transformation. Each treatment is repeated three times to take the average value.

[0099] Table 4 Full bionic simulation of solid waste combination optimization test

[0100]

[0101]

[0102] Test results:

[0103] The parameter changes of different full-bionic simulations affect the effective phosphorus content of solid waste and the production of organic acids. Compared with the control 8 and 9 without adding sludge or fly ash (or desulfurization ash), the effective phosphorus content of the decomposition solid waste reaction of the full-bionic simulation formula of other experimental examples is relatively high. Among them, the best effect is achieved when the volume ratio of kitchen waste: sludge: fly ash (or desulfurization ash) is 100:50:30. The adsorption capacity of coal gangue after microbial modification is extremely strong, and phosphorus in fixed phosphate is directly released. The content of organic acid (oxalic acid) is also the highest in experimental example 10. Oxalic acid is the most representative type of organic acid and one of the important organic acids required for plant growth. Through the modification of solid waste coal gangue by microorganisms and full-bionic swamps, the organic acid in the solid waste is released, thereby promoting plant growth.

[0104] During the reaction process, we found that the control of water content, salinity, and carbon-nitrogen ratio in the full bionic simulated swamp reaction is very important, and it has different effects on the fermentation effect of solid waste. After 60 days of modified solid waste test field, it was found that the fermentation decomposition effect was the highest when the carbon-nitrogen ratio was adjusted to 35:1, the salinity was 20%, and the water content was 60%. It shows that the effect of phosphorus dissolution and promotion of organic acid production in the acidic solid waste matrix environment is good. The effective phosphorus content and acid production in this process are both high. At the same time, this parameter can maintain an acidic environment and promote the lasting decomposition of microorganisms. A high carbon-nitrogen ratio is not conducive to the acid production reaction of microorganisms. When it is 25-35:1, more organic acids can be produced, thereby promoting the decomposition of solid waste. Excessive and low salt concentrations also affect the acid production process. When the salt content is about 20%, the acid production effect is better. The water content of the bionic simulated swamp mixture is also an important factor affecting the microbial fermentation reaction. When the water content is 60%, its fermentation reaction to solid waste is better.

[0105] Experiment 4: Optimization scheme of full bionic headband combination

[0106] Test method: The full bionic cover combination of the present invention cooperates with microorganisms to produce organic acids during the growth and reproduction process, uses bionic simulation of the natural generation process of organic carbon mines, combines biological fermentation acid production conditions, and uses the carbon-nitrogen ratio, pH, and salinity in the waste liquid to regulate the acid production. This experiment mainly studies the combination of full bionic covers to optimize the process parameter conditions, specifically laying a bionic swamp cover on the top layer of the solid waste stacking layer, and adjusting the components of the bionic swamp cover and the environment to control the anaerobic fermentation environment of the lower bionic swamp mixture and solid waste, and provide the most suitable anaerobic fermentation conditions for it. The specific method is shown in Example 1, and the conditions suitable for solid waste fermentation are obtained by comparing the covers of different ratios in the top layer of the bionic swamp mixture. Each treatment was repeated three times and the average value was taken. After 60 days of fermentation, the ammonia nitrogen content (mg / L) of the mixture layer and the effective phosphorus content (mg / kg) of the bottom solid waste layer were measured.

[0107] Table 5. Optimization test table of full bionic head covering simulation parameters

[0108]

[0109] Test results: The simulated bionic cover can moderately keep warm and moisturize. The anaerobic fermentation conditions can be further adjusted by controlling the temperature of the cover. Because a lot of heat will be generated during the anaerobic fermentation process, the simulated bionic cover can maintain a certain temperature, and its temperature should not be too high to affect the microbial fermentation. Humidity regulation is also a key factor in further maintaining the anaerobic fermentation of solid waste. Through the experiment, we found the appropriate temperature and humidity that affect the fermentation of the bionic swamp mixture, that is, the temperature is 30℃ and the humidity is 60%. The effect is better. This regulation is suitable for the composting fermentation of the lower bionic swamp mixture, and the ammonia nitrogen release is as high as 665.61mg / L, and the effective phosphorus release of solid waste is as high as 853.21mg / kg.

[0110] The simulated bionic lid of the present application is obtained through a large number of experimental comparisons. Its optimal component ratio is that the straw and mushroom residue are matched in a volume ratio of 3:2, and the fermentation effect is optimal when the crushed particle size is 3 cm. Referring to reference 21 and 22, compared with the simulated bionic lid composed of adding only one ingredient, the simulated bionic lid of the present application has the characteristics of better heat preservation, moisture retention and fermentation promotion effect after combination.

[0111] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. Application of Pseudomonas fluorescens N1 in combination with coal gangue materials in modifying solid waste, characterized in that: The steps include: (1) Magnesium slag and coal gangue are added and mixed thoroughly, and the mixture is sieved. The amount of coal gangue added is 10% of the volume of the magnesium slag. The particle size of the mixed material is controlled at 2 mm and the mixture is stacked for 8 days; (2) adding vinegar dregs in an amount of 30% of the volume of the magnesium slag, mixing well, and adjusting the pH value to 5.5 using wood vinegar; (3) Spray an equal volume of solution of Pseudomonas fluorescens N1 + Xanthomonas campestris NRRLB-1459 on the surface of the material, with a daily spraying volume of 1.00 m 3 / m 3 , lasting 35 days; The Pseudomonas fluorescens N1 has a registration number of CGMCC No. 23192 in the General Microbiological Center of China National Microbiological Culture Collection.

2. A method for resource utilization of solid wastes by combining Pseudomonas fluorescens N1 with full bionic simulation, characterized in that: The steps include: (1) Coal gangue with a particle size of 3 mm is piled at the bottom, with a thickness of 6 m; (2) stacking a bionic swamp mixture on the first layer of coal gangue, specifically a mixture of sludge, food waste and fly ash, adjusting the water content of the mixture so that the fermentation water meets the basic requirements of anaerobic fermentation; at the same time, the water content of the mixture is controlled at 60%, the carbon-nitrogen ratio is adjusted to 35:1, the pH value is 5.5, the salinity is 20%, the stacking thickness is 3m, and anaerobic fermentation is performed; wherein the volume ratio of food waste: sludge: fly ash is 10:5:5; (3) alternately repeating steps (1)-(2), performing multi-layer stacking according to the stacking processing volume, thereby improving the stacking efficiency and the total processing volume; (4) A bionic swamp cover is laid on the top layer of the stacking layer. The bionic swamp cover is composed of straw and mushroom residue in a volume ratio of 3:

2. During the anaerobic fermentation process, functional microbial eluent, carbon-nitrogen ratio eluent, pH adjustment eluent and high-salt eluent are transported to the stacking layer below. At the same time, the bionic swamp cover is used to adjust the wet heat storage energy of the bottom stacking layer. A sunlight film is set on the bionic swamp to concentrate energy. The microorganism in the microbial eluent is Pseudomonas fluorescens N1, and the daily spraying water volume is 1.00m 3 / m 3 , lasting for 60 days; the carbon-nitrogen ratio eluent is a conventional solution with a carbon-nitrogen ratio of 35:

1. The rich carbon-nitrogen ratio ensures that the anaerobic fermentation stays in the acid-producing stage; the pH eluent is added according to the probe feedback. When the pH is less than 4.6, alkaline waste liquid is added, and when the pH is greater than 6.0, acidic waste liquid is added; the salt content in the high-salt eluent is 20%; The Pseudomonas fluorescens N1 has a registration number of CGMCC No. 23192 in the General Microbiological Center of China National Microbiological Culture Collection.

3. The method according to claim 2, characterized in that The method also includes step (5), wherein a sensing probe is arranged at the bottom of the bionic swamp cover to timely feedback the temperature and humidity of the fermentation pile. When the temperature is too high or too low, the temperature is controlled by delivering the above-mentioned eluent at a certain temperature. Specifically, the temperature is maintained at 30°C and the humidity is maintained at 60%.

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