Identification of InDel molecular markers for resistance to Fusarium spp. in maize and its application

Abstract

The present invention provides an InDel molecular marker for identifying resistance to Fusarium truncatum in corn species. The Fusarium truncatum resistance trait gene in corn species is located in the promoter region of the GRMZM2G083526 gene on corn chromosome 5, including two tightly linked InDel molecular markers, InDel-329 and InDel-395, respectively. The nucleotide sequence of InDel-329 is CATGTCC, and the nucleotide sequence of InDel-395 is CATCGAC, both of which show insertion / deletion polymorphisms. The present invention also provides an application of InDel molecular markers for use in a kit for detecting resistance to Fusarium truncatum in corn species, analysis of corn germplasm resources, or assisted genetic breeding. The present invention can effectively accelerate the corn seed breeding process.

Description

Technical Field

[0001] The invention belongs to the technical field of molecular genetics, and particularly relates to an InDel molecular marker for identifying resistance to Fusarium oxysporum f. verticillioides in corn seeds and an application thereof. Background Art

[0002] Maize is the largest and most productive grain crop in my country, playing a vital role in national food security. Internal infection of maize seeds by Fusarium spp. is widespread, leading to mold growth during storage, significantly shortening the shelf life. Furthermore, the accumulation of mycotoxins within the seeds poses a serious threat to human and animal health. If asymptomatically infected kernels are used as seed for production the following year, they become a new source of infection, leading to a new round of major diseases such as stalk rot and ear rot, resulting in severe yield losses. Intraspecific resistance to Fusarium spp. differs from conventional seed resistance, and breeders often develop resistant varieties with poor intraspecific resistance phenotypes. This is due to the difficulty in selecting resistant parent germplasm resources and a lack of understanding of the genetic mechanisms of resistance to intraspecific pathogenic fungi. The cumbersome identification of intraspecific resistance phenotypes in maize, coupled with a scarcity of high-quality genes and associated functional alleles, has resulted in a scarcity of high-quality germplasm resources, severely limiting the progress of molecular breeding efforts.

[0003] Insertion-deletion markers (InDels) refer to the insertion or deletion of nucleotide fragments at the same genomic site in different individuals of the same species, or in the case of maize, different strains within a naturally differentiated population. This creates gaps in some individuals during homologous alignment. In naturally differentiated maize populations, InDel variation is widespread and numerous. InDel polymorphic molecular markers are PCR-amplified markers designed based on the insertion or deletion site or the insertion or deletion fragment itself, capable of distinguishing the phenotype of the same trait between different individuals. They facilitate genotyping using electrophoresis platforms and predict the phenotype of associated traits, playing a vital role in molecular-assisted genetic breeding of maize. InDel molecular markers are highly accurate, stable, and easy to detect, making them widely used in molecular-assisted genetic breeding and genomic association analysis of crops. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide an InDel molecular marker for identifying resistance to Fusarium oxysporum in corn species and its application in view of the deficiencies of the above-mentioned prior art. The present invention uses whole-genome association analysis to accurately detect the GRMZM2G083526 gene site that is significantly associated with the resistance trait of Fusarium oxysporum in corn species. Further, through promoter sequencing, it is clarified that: in the naturally differentiated population, there are two completely linked InDel allelic variations on the promoter of the GRMZM2G083526 gene, which are related to the transcriptional expression of the GRMZM2G083526 gene, thereby controlling the difference in resistance of different corn strains to Fusarium oxysporum. The above-mentioned InDel variation site is used as a genetic marker, which can be applied to the screening of germplasm resources with excellent resistance to Fusarium oxysporum in species, accelerate the relevant breeding process, and has a high application value.

[0005] To solve the above technical problems, the technical solution adopted by the present invention is: an InDel molecular marker for identifying resistance to Fusarium truncatum in maize, characterized in that the gene for resistance to Fusarium truncatum in maize is located in the promoter region of the GRMZM2G083526 gene on maize chromosome 5, and includes two closely linked InDel molecular markers, InDel-329 and InDel-395, both of which have two homozygous genotypes of insertion and deletion;

[0006] The InDel-329 molecular marker consists of 7 nucleotides and is located at position 1531bp of Chr5_9034 in the B73 reference genome. The nucleotide sequence of the InDel-329 molecular marker is: CATGTCC. When the resistance to Fusarium spp. in corn is weak, it is an insertion genotype, and when the resistance to Fusarium spp. in corn is strong, it is a deletion genotype.

[0007] The InDel-395 molecular marker consists of 7 nucleotides and is located at the Chr5_90341465bp position of the B73 reference genome; the nucleotide sequence of the InDel-395 molecular marker is: CATCGAC, which is an insertion genotype when the resistance to Fusarium spp. in corn is weak, and a deletion genotype when the resistance to Fusarium spp. in corn is strong;

[0008] The primer pair of the InDel molecular marker consists of a forward primer and a reverse primer. The primer pair is located at the flanking sequence within 500 bp of both ends of the InDel molecular marker, including a segment spanning the InDel molecular marker or a single-stranded DNA matching the InDel molecular marker.

[0009] The present invention also provides a method for detecting InDel molecular markers for identifying resistance to Fusarium spp. in corn, characterized in that the method comprises:

[0010] Extracting corn genomic DNA, and performing PCR amplification on the extracted corn genomic DNA using the primer pair labeled with the InDel molecule to obtain a PCR product;

[0011] The PCR amplification reaction system is: KOD FX Neo MIX 10 μL, forward primer (10 μmol / L) 1 μL, reverse primer (10 μmol / L) 1 μL, corn genomic DNA 1 μL, ddH2O 7 μL;

[0012] The PCR amplification reaction program was as follows: pre-denaturation at 98°C for 3 min; denaturation at 98°C for 10 s, annealing at 55°C for 5 s, and extension at 68°C for 10 s, for a total of 35 cycles; and extension at 68°C for another 10 min.

[0013] After PCR amplification, the amplified products were separated by agarose electrophoresis, the specific primers were recovered and purified, and first-generation DNA sequencing was performed, and sequence alignment was performed using DNAman; the genotype with a seven-base insertion of CATGTCC at the physical position Chr5_90341531bp on the GRMZM2G083526 promoter, and the seven-base insertion of CATCGAC at the physical position Chr5_90341465bp were identified as susceptible materials to intraspecific Fusarium spp.; the genotype with a seven-base deletion of CATGTCC at the physical position Chr5_90341531bp on the GRMZM2G083526 promoter, and the seven-base deletion of CATCGAC at the physical position Chr5_90341465bp were identified as resistant materials to intraspecific Fusarium spp.

[0014] The present invention also provides an application of an InDel molecular marker for identifying resistance to Fusarium oxysporum in corn species, characterized in that the InDel molecular marker for identifying resistance to Fusarium oxysporum in corn species can be applied to a kit for detecting resistance to Fusarium oxysporum in corn species, corn germplasm resource analysis, or corn assisted genetic breeding.

[0015] The corn intraspecific Fusarium spp. resistance InDel molecular markers of the present invention are accurate and stable, and do not require redundant steps such as enzyme cutting. The polymorphism of the InDel molecular markers is carried out at the genomic DNA level and can be detected in different developmental stages and different tissues and organs of corn, especially for identification at the seedling stage, which can effectively accelerate the corn breeding process.

[0016] The InDel molecular marker primer pair and kit of the present invention have stable amplification products, strong specificity and high sensitivity.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] 1. The present invention uses whole-genome association analysis to accurately detect the GRMZM2G083526 gene locus, which is significantly associated with the resistance trait to Fusarium wilt in maize. Further promoter sequencing confirmed that in naturally differentiated populations, there are two completely linked InDel allelic variants on the GRMZM2G083526 gene promoter, which are associated with the transcriptional expression of the GRMZM2G083526 gene, thereby controlling the differences in resistance to Fusarium wilt in different maize strains. Using the above-mentioned InDel variant sites as genetic markers, it can be applied to the identification of resistance traits to Fusarium wilt in maize species and the screening of germplasm resources with excellent resistance.

[0019] 2. The indel molecular markers associated with resistance to Fusarium spp. within corn species of the present invention are accurate and stable, and do not require tedious steps such as enzyme digestion. The polymorphism of the indel molecular markers described in the present invention is detected at the genomic DNA level and can be detected in different developmental stages and different tissues and organs of corn, especially at the seedling stage, which can effectively accelerate the corn breeding process.

[0020] 3. The InDel molecular marker primer pair and kit described in the present invention have stable amplification products, strong specificity, and high sensitivity, and can be widely used in maize germplasm resource analysis and molecular-assisted genetic breeding, with high application value.

[0021] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a SNP variation site associated with the resistance phenotype of Fusarium truncatum identified by genome-wide association analysis.

[0023] Figure 2 This is a single gene association analysis between SNPs in the GRMZM2G083526 gene interval and intraspecific resistance to Fusarium oxysporum.

[0024] Figure 3 Intraspecific Fusarium spp. resistance haplotype analysis based on the significant SNP of the GRMZM2G083526 gene.

[0025] Figure 4 Single gene association analysis of intraspecific Fusarium truncatum resistance with the promoter InDel variation of the GRMZM2G083526 gene.

[0026] Figure 5 This is an intraspecific haplotype analysis of resistance to Fusarium truncatum based on the InDel variation in the GRMZM2G083526 gene promoter.

[0027] Figure 6It is a dual fluorescence reporter system to identify the functional variation of GRMZM2G083526 gene promoter InDel.

[0028] Figure 7 It is a sequencing comparison of genotyping of different maize families using the InDel molecular markers of the present invention. DETAILED DESCRIPTION Example 1

[0029] This example describes the location, cloning, and molecular marker development of a set of InDel genetic variants highly linked to resistance to Fusarium spp. in maize. The following is the specific process for cloning and developing the molecular markers:

[0030] 1. Targeting groups

[0031] 170 temperate, tropical and subtropical maize inbred lines with CIMMYT, Reid, P Group and TSPT bloodlines were collected to construct an associated population for positioning, which has rich genetic diversity.

[0032] 2. Field trial design

[0033] A mapping population of 170 maize inbred lines was planted in the field in 2019 in Maozhuang Village, Zhengzhou City (N34°87′, E113°61′), Xingyang City (N34°89′, E113°36′), Zhongmu County (N34°74′, E114°12′), and an experimental field at the Xuchang Campus of Henan Agricultural University (N34°02′, E113°50′). The field experiment used a randomized block design with two replicates per design. Each line and each replicate was planted in two rows, each 3 m long, with 12 plants per row and 50 cm spacing between rows. Field management followed local standards for fertilization, watering, and weeding. Planting materials were hand-pollinated, and 15 days after pollination, the ears were inoculated with Fusarium pseudo-verticillium to introduce the fungus into the maize seeds.

[0034] 3. Inoculation of Fusarium spp.

[0035] S1. Cultivation of Fusarium spp.: Inoculate Fusarium spp. retained in the laboratory onto potato dextrose agar (PDA) medium and culture in a constant temperature incubator at 28°C in the dark for 5-7 days. Once the entire plate is fully grown, store in a refrigerator at 4°C until ready for use.

[0036] S2. Propagation of Fusarium spp.: Before inoculation in the field, propagate Fusarium spp. on corn broth medium (CSM) to produce a large number of microspores. The method is as follows: in a sterile workbench, add two small 1 cm × 1 cm pieces of freshly cultured PDA culture medium filled with bacteria to the corn broth, shake thoroughly, and then place in a constant temperature incubator and incubate in the dark at 28°C for 7-10 days; absorb the spores with deionized water, filter the eluate through 8 layers of gauze, and adjust the concentration to 5 × 10 6 / mL, and then Tween-80 surfactant was added to a final concentration of 2 μL / mL to obtain a spore suspension for artificial inoculation;

[0037] S3. 15 days after artificial pollination of the maize population material, the spore suspension prepared in S2 was inoculated on the young ears, with 2 mL of spore suspension inoculated per ear; 45 days after artificial pollination, the artificially inoculated ears were harvested and naturally aired for identification of resistance to Fusarium oxysporum f.

[0038] The preparation method of the potato dextrose agar (PDA) medium is as follows:

[0039] (1) Take 200g of fresh potatoes without sprouts, peel them, wash them, and cut them into small pieces. Add 1L of distilled water, boil them for 20min, filter them twice with 8 layers of gauze, and place them in a 2000mL beaker.

[0040] (2) Add 20 g of anhydrous glucose and dissolve it fully while hot. After cooling, divide it equally into three 500 mL conical flasks, add 5 g of agar powder to each flask, and seal it with two layers of breathable sealing film;

[0041] (3) Sterilize with high pressure steam at 121°C for 30 minutes;

[0042] (4) Take out the sterilized and cooled PDA culture medium to 50-60℃ and pour it into 9cm culture dishes in a clean bench, 15-20mL per dish. After the culture medium in the dish is completely condensed, cover the dish with a lid and seal it with a sealing film. The entire operation is carried out in a sterile workbench. The poured culture medium is placed at room temperature for 48 hours. If there is no growth of bacteria, it can be used or stored at 4℃ for later use.

[0043] The preparation method of the corn culture medium (CSM) is as follows:

[0044] (1) Weigh 1000 g of corn hybrid seeds with full kernels, wash them 2-3 times with tap water and 1-2 times with distilled water; add 3 L of distilled water and boil for 1-2 hours;

[0045] (2) Drain the cooked corn kernels and place them into 500 mL conical flasks, filling each flask to the 200 mL mark;

[0046] (3) Seal with two layers of breathable sealing film and wrap it tightly with a rubber band;

[0047] (4) Sterilize at 121°C for 30 minutes. After sterilization, place at room temperature for 48 hours and keep the sample free of contamination from other bacteria.

[0048] 4. Identification of resistance traits within related populations

[0049] Ten disease-free seeds from each sun-dried seed cluster surrounding the inoculation site were collected for phenotypic identification. After surface disinfection with a 10% NaClO solution, the seeds were placed embryo-side down on absorbent paper and kept in the dark at 28°C for 5 days. Disease incidence was assessed and graded into five levels (1, 3, 5, 7, and 9). See Table 1 for specific indicators.

[0050] Table 1 Seed disease grading standards

[0051]

[0052] *Note: There are 10 seeds per dish. NDS represents the number of diseased seeds. IA represents the ratio of the diseased area of ​​a single seed to the total area of ​​a single seed.

[0053] 5. Genome-wide association study (GWAS)

[0054] The above statistical disease levels were converted to BLUE values ​​using QTL IciMapping software. Combined with the high-density single nucleotide polymorphism (SNP) molecular markers of the associated population, TASSEL 5.0 software was used to perform a genome-wide association analysis of the phenotype BLUE using the MLM model. The allele frequency threshold was set at 0.05, and the allele frequency was 0.05 when p < 10 -5 The significance of SNP-trait association was determined at the level of

[0055] The results showed that the significant SNPs included two SNPs at positions chr5_90344973 and chr5_90344987 ( Figure 1 ), which was significantly associated with the resistance to Fusarium spp. within the species at p = 1.09 × 10 -6 , among which the chr5_90344973 allele is T / G, and the chr5_90344987 allele is C / T. Both SNPs are located in the fourth intron of the GRMZM2G083526 gene, and these two SNP variants are closely linked in the test population ( Figure 2 ), there are two homozygous genotypes, CT / CT and TG / TG, in the inbred lines of the test population. Haplotype analysis showed that the variation at this site is closely linked to resistance to Fusarium spp. within the species ( Figure 3 ).

[0056] 6. Identification of functional variation of InDel in the promoter of GRMZM2G083526 gene

[0057] As mentioned above, the region where the GRMZM2G083526 gene is located is closely related to the species Fusarium oxysporum. To determine the functional variation of the GRMZM2G083526 gene, the GRMZM2G083526 promoter region of 112 population materials was cloned and aligned, and two InDel mutation sites (InDel-395 and InDel-329) were found within 500bp upstream of the ATG of the GRMZM2G083526 gene. Among them, InDel-329 is a "CATGTCC" seven-base insertion / deletion variation with a physical location of Chr5_90341531bp; InDel-395 is a "CATCGAC" seven-base insertion / deletion variation with a physical location of Chr5_90341465bp. Association analysis of the GRMZM2G083526 gene combined with a high-density SNP map found that the two InDel mutations were completely linked ( Figure 4 ), and also has a high degree of linkage with the SNP sites of chr5_90344973 and chr5_90344987 ( Figure 4 ), because these two InDel variants are located in the promoter region, and haplotype analysis shows that these two InDel variants are closely linked to the disease grade ( Figure 5 Sequence analysis of the InDel mutation sites in different inbred lines revealed that the disease grade of the inbred lines with insertion genotypes of InDel-329 and InDel-395 (susceptible materials) was significantly higher than that of the inbred lines with deletion genotypes (resistant materials) (Table 2), indicating that these two InDel mutations may be functional mutations of the GRMZM2G083526 gene involved in intraspecific resistance to Fusarium spp.

[0058] Table 2 Intraspecific resistance phenotypes and InDel-329 / InDel-395 genotypes of different maize inbred lines

[0059] ,

[0060] ,

[0061] ,

[0062] ,

[0063]

[0064] 7. Molecular marker development for GRMZM2G083526 promoter InDel mutation

[0065] Furthermore, to apply variations in the GRMZM2G083526 promoter for disease-resistant germplasm screening and genetic breeding, specific primers were designed for the promoter. These primers comprise a forward primer and a reverse primer pair for sequencing and typing. This primer pair spans two InDel molecular markers in the GRMZM2G083526 promoter and is used to clone the promoter sequence 500 bp upstream of the ATG start codon of GRMZM2G083526, enabling genotyping via first-generation DNA sequencing. Example 2

[0066] This example is to identify the effect of GRMZM2G083526 gene promoter InDel molecular marker on gene expression:

[0067] The GRMZM2G083526 promoter with an InDel deletion and an InDel insertion were cloned from the genomic DNA of the inbred line CML192, which has strong resistance to Fusarium spp., and the inbred line WIL901, which has weak resistance to Fusarium spp., respectively. The promoters were constructed into the pGreen0800II dual-luciferase reporter vector and transformed into maize mesophyll protoplasts. The promoter activity levels of different genotypes were reflected by measuring the RLUC / FLUC value. The results showed that the GRMZM2G083526 promoter sequence with an InDel deletion had stronger transcriptional activity ( Figure 6 ), indicating that these two InDel molecular markers are involved in the resistance to Fusarium oxysporum in maize by controlling the expression level of GRMZM2G083526. Example 3

[0068] This example is an application test of InDel molecular markers in identifying resistance traits to Fusarium spp. in corn:

[0069] Four intraspecific Fusarium spp.-resistant accessions (CML505, CML192, CNW025, and CNW053) and four susceptible accessions (J8606, WIL901, LH51, and CNW139) were selected from the linkage population. B73, an inbred line with deletion genotypes at both InDel markers, was used as a control. Specific primers flanking the regions containing these two markers were designed and PCR amplified using KOD FX Neo high-fidelity polymerase (Toyobo (Shanghai) Biotechnology Co., Ltd.) and maize genomic DNA as a template. Amplified products were separated by agarose gel electrophoresis, and specific bands were recovered and purified using a gel extraction kit (OmegaBio-Tek) before being subjected to next-generation sequencing.

[0070] The amplification system comprises: 10 μL of KOD FX Neo MIX, 1 μL of forward primer (10 μmol / L), 1 μL of reverse primer (10 μmol / L), 1 μL of corn genomic DNA template, and 7 μL of ddH2O.

[0071] The amplification program was as follows: pre-denaturation at 98°C for 3 min; denaturation at 98°C for 10 s, annealing at 55°C for 5 s, and extension at 68°C for 10 s, for a total of 35 cycles; and further extension at 68°C for 10 min.

[0072] The corn genomic DNA extraction process is as follows:

[0073] (1) Using maize inbred line seedlings at the three-leaf stage as the material, grind into powder with liquid nitrogen, dispense into 2 mL centrifuge tubes, add 800 μL of CTAB (containing 2% mercaptoethanol) solution preheated at 60-65°C, and incubate in a water bath at 60-65°C for 60 min. Gently shake up and down every 5-10 min to prevent the sample from agglomerating in the tube.

[0074] (2) After cooling to room temperature, add 800 μL of a mixed solution of chloroform and isoamyl alcohol (chloroform:isoamyl alcohol = 24:1) and mix gently up and down manually for 5–10 minutes until the solution is clearly separated into three layers. Centrifuge at 25°C and 12,000 × g / min for 8–10 minutes.

[0075] (3) Aspirate 600 μL of the supernatant into a new 2.0 mL centrifuge tube, add 1.5 μL of RNase (10 mg / mL), and incubate at 37°C for 1 h.

[0076] (4) Add 600 μL of a mixture of chloroform and isoamyl alcohol (chloroform:isoamyl alcohol = 24:1), shake manually up and down for 5 min, and centrifuge at room temperature and 12,000 × g / min for 8 min;

[0077] (5) Aspirate 400 μL of supernatant into a 1.5 mL centrifuge tube, add 400 μL of isopropanol, mix thoroughly, let stand at -20°C for 30 min, and centrifuge at 12,000 × g / min for 8 min;

[0078] (6) Wash the precipitate twice with 75% ethanol, centrifuge at 12,000 × g / min for 5 min, and discard the supernatant;

[0079] (7) After the DNA precipitate is dried, add 100 μL of deionized water to dissolve it and store it at -20°C.

[0080] DNAman software was used to compare the sequencing results ( Figure 7), the results showed that this InDel molecular marker was able to successfully genotype eight maize seeds with different resistance to Fusarium spp. Among them, the inbred lines CML505, CML192, CNW025, and CNW053 all had deletion genotypes at markers InDel-329 and InDel-395, indicating strong resistance to Fusarium spp. within the species. The inbred lines J8606, WIL901, LH52, and CNW139 all had insertion genotypes at markers InDel-329 and InDel-395, indicating weak resistance to Fusarium spp. within the species.

[0081] The InDel molecular marker for identifying resistance to Fusarium tumefaciens in corn can be applied to identification of resistance traits to Fusarium tumefaciens in corn, a detection kit for resistance traits to Fusarium tumefaciens in corn, analysis of corn germplasm resources and corn assisted genetic breeding.

[0082] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent variation made to the above embodiment based on the essence of the invention technology shall still fall within the scope of protection of the technical solution of the present invention.

Claims

1. An InDel molecular marker for identifying resistance to Fusarium spp. in corn, characterized in that: The corn species-specific Fusarium spp. resistance gene is located in the promoter region of the GRMZM2G083526 gene on maize chromosome 5, including two closely linked InDel molecular markers, InDel-329 and InDel-395, which are physically located at Chr5_90341531bp and Chr5_90341465bp, respectively, and the reference genome is B73; The InDel-329 molecular marker consists of 7 nucleotides, and the nucleotide sequence is: CATGTCC; The InDel-395 molecular marker consists of 7 nucleotides, and the nucleotide sequence is: CATCGAC.

2. A method for identifying resistance to Fusarium spp. in corn using the InDel molecular marker according to claim 1, characterized in that: The method is: Extracting corn genomic DNA, performing PCR amplification on the extracted corn genomic DNA using primer pairs labeled with InDel molecules to obtain PCR products; The PCR amplification reaction system is: KOD FX Neo MIX 10 μL, 1 μL of 10 μmol / L forward primer, 1 μL of 10 μmol / L reverse primer, 1 μL of corn genomic DNA, and 7 μL of ddH2O; The PCR amplification reaction program was as follows: pre-denaturation at 98°C for 3 min; denaturation at 98°C for 10 s, annealing at 55°C for 5 s, and extension at 68°C for 10 s, for a total of 35 cycles; and extension at 68°C for another 10 min. After PCR amplification, the amplified products were separated by agarose electrophoresis, the specific primers were recovered and purified, and first-generation DNA sequencing was performed, and sequence alignment was performed using DNAman; the genotype with a seven-base insertion of CATGTCC at the physical position Chr5_90341531bp on the GRMZM2G083526 promoter, and the seven-base insertion of CATCGAC at the physical position Chr5_90341465bp were identified as susceptible materials to intraspecific Fusarium spp.; the genotype with a seven-base deletion of CATGTCC at the physical position Chr5_90341531bp on the GRMZM2G083526 promoter, and the seven-base deletion of CATCGAC at the physical position Chr5_90341465bp were identified as resistant materials to intraspecific Fusarium spp.

3. A use of the InDel molecular marker for identifying resistance to Fusarium spp. in corn as claimed in claim 1, characterized in that: The InDel molecular marker for identifying resistance to Fusarium spp. in corn is applied to a kit for detecting resistance to Fusarium spp. in corn.

4. A use of the InDel molecular marker for identifying resistance to Fusarium spp. in corn as claimed in claim 1, characterized in that: The InDel molecular marker is used for analysis of corn germplasm resources related to intraspecific Fusarium spp. resistance traits or corn assisted genetic breeding.

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