A sheath fluid for urine analysis and preparation method thereof

Abstract

The present application relates to the technical field of urine analysis, and specifically discloses a sheath fluid for urine analysis and a preparation method thereof. The sheath fluid for urine analysis includes water, 2-carbamoyl-3-nitrobenzoic acid, an osmotic pressure adjuster and 2-amino-1,3-propylene glycol hydrochloride. In the sheath fluid, 2-carbamoyl-3-nitrobenzoic acid contains carboxyl, carbamoyl and nitro groups adjacent to the benzene ring, the carboxyl group can dissociate hydrogen ions, the amino group and the nitro group can accept hydrogen ions, and 2-amino-1,3-propylene glycol hydrochloride has hydroxyl groups at both ends and an amino group in the middle and a group bonded to hydrochloric acid on the propane chain, which buffers pH together. The amino groups of 2-carbamoyl-3-nitrobenzoic acid and 2-amino-1,3-propylene glycol hydrochloride can also be attached to the surface of pathogens, preventing the pathogens from absorbing nutrients, thereby inhibiting the growth of pathogens. 2-Carbamoyl-3-nitrobenzoic acid can also chelate osmotic pressure regulators, etc., making it difficult to precipitate and adhere to the urine analyzer.

Description

Technical Field

[0001] The present application relates to the technical field of urine analysis, and more specifically, to a sheath fluid for urine analysis and a preparation method thereof. Background Art

[0002] Urinalysis, also known as urine analysis, is defined as the analysis of urine by visual inspection, physics, chemistry, microscopy and instruments for the purpose of preventing, diagnosing, observing therapeutic effects and judging the prognosis of urinary, circulatory, liver, gallbladder and endocrine diseases.

[0003] The fully automatic urine analyzer uses flow imaging technology to provide high-definition images of urine formed elements. It can automatically identify a variety of formed elements in urine, such as red blood cells, white blood cells, white blood cell clusters, squamous epithelial cells, non-squamous epithelial cells, hyaline casts, pathological casts, bacteria, yeast, unclassified crystals, sperm, mucus threads, etc., to analyze symptoms such as hematuria, crystalluria, and pyuria.

[0004] The fully automatic urine analyzer uses sheath liquid as the carrier fluid, and injects urine into the sheath liquid flow to form a core-shell liquid flow in which the sheath liquid wraps the urine. The liquid flow plane is then spread out through a flat liquid flow channel, so that the formed components in the urine can be clearly seen from the front to analyze the formed components in the urine. No centrifugation and staining are required, and the test results are highly reliable. However, the composition of some current sheath liquids used for urine testing is relatively complex, for example, a mixture of multiple components such as antibacterial agents, pH buffers, defoaming agents, chelating agents, etc., and the properties are unstable. Summary of the invention

[0005] In order to improve the problem that the urine components used in some current urine tests are relatively complex and unstable in nature, the present application proposes a sheath fluid for urine analysis and a preparation method thereof.

[0006] In a first aspect, the present application proposes a sheath fluid for urine analysis and adopts the following technical solution.

[0007] A sheath fluid for urine analysis comprises water, 2-carbamoyl-3-nitrobenzoic acid, an osmotic pressure adjuster and 2-amino-1,3-propanediol hydrochloride.

[0008] By adopting the above technical scheme, 2-aminoformyl-3-nitrobenzoic acid contains adjacent carboxyl, carbamoyl and nitro groups on the benzene ring, the carboxyl group can dissociate hydrogen ions, the amino group and the nitro group can accept hydrogen ions, and play the role of acid-base buffering, and 2-amino-1,3-propanediol hydrochloride has hydroxyl groups at both ends and amino groups in the middle and groups bonded to hydrochloric acid on the propane chain, hydrochloric acid can dissociate hydrogen ions, the amino group in the middle can accept hydrogen ions, and also play the role of acid-base buffering, 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride can also accept hydrogen ions from each other to balance pH together. The amino groups of 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride can also attach to the surface of pathogens to prevent the pathogens from absorbing nutrients, and the nitro groups on the benzene ring destroy the cell membrane, thereby inhibiting the growth of pathogens, so that the sheath fluid meets the requirements of pathogen control. By adjusting the concentration of 2-carbamoyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride, the absorbance of the sheath liquid can also be adjusted so that the sheath liquid meets the use requirements of the urine analyzer. The adjacent carboxyl, carbamoyl and nitro groups on 2-carbamoyl-3-nitrobenzoic acid can also chelate osmotic pressure adjusters, etc., so that it is not easy to precipitate and adhere to the urine analyzer. By adjusting the concentration of 2-carbamoyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride, the viscosity of the sheath liquid can also be adjusted so that the sheath liquid can wrap the urine during the test, so that the urine flows along the middle of the pipe, and will not dissolve with the urine too quickly, which is convenient for observing the tangible objects in the urine and improving the reliability of the test results. 2-carbamoyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride also have a certain defoaming effect, reducing the probability of bubbles in the sheath liquid.

[0009] As an improvement of the sheath fluid for urine analysis, the osmotic pressure adjuster is sodium chloride and / or potassium chloride, and the total concentration of the osmotic pressure adjuster is 4 to 9 g / L.

[0010] By adopting the above technical solution, the osmotic pressure of the sheath fluid is between 260 and 330 mOsm / (kg·H2O), which is close to the osmotic pressure of general plasma, thereby maintaining the osmotic pressure environment of tangible objects such as red blood cells in urine, reducing the probability of water loss or swelling of tangible objects such as red blood cells, and maintaining activity for easy observation and analysis.

[0011] As an improvement of the sheath fluid for urine analysis, the concentration of the 2-carbamoyl-3-nitrobenzoic acid is 3 to 10 g / L.

[0012] By adopting the above technical scheme, 2-carbamoyl-3-nitrobenzoic acid contains carboxyl, carbamoyl and nitro groups adjacent to each other on the benzene ring. The carboxyl group can dissociate hydrogen ions, and the amino and nitro groups can accept hydrogen ions. At a concentration of 3 to 10 g / L, it can comprehensively play the roles of acid-base buffering, antibacterial, chelating osmotic pressure regulator, defoaming, etc. It also has the role of regulating the viscosity and absorbance of the sheath fluid. If the concentration is too high or too low, the efficiency of these comprehensive effects will be reduced.

[0013] As an improvement of the sheath fluid for urine analysis, the concentration of the 2-amino-1,3-propanediol hydrochloride is 0.5 to 6 g / L.

[0014] By adopting the above technical scheme, 2-amino-1,3-propanediol hydrochloride has hydroxyl groups at both ends and an amino group in the middle and a group bonded to hydrochloric acid on the propane chain. Hydrochloric acid can dissociate hydrogen ions, and the amino group in the middle can accept hydrogen ions, which comprehensively play the role of buffering pH, inhibiting bacteria, and defoaming. It also has the function of complexing osmotic pressure regulators to prevent their precipitation and regulating the viscosity and absorbance of sheath fluid. If the concentration is too high or too low, the efficiency of these comprehensive effects will be reduced.

[0015] As an improvement of the sheath fluid for urine analysis, the concentration of the 2-carbamoyl-3-nitrobenzoic acid is 6 to 7 g / L, and the concentration of the 2-amino-1,3-propanediol hydrochloride is 2.5 to 4 g / L.

[0016] By adopting the above technical scheme, at the combined concentration, 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride cooperate with each other, so that the sheath fluid has better absorbance, pH buffering effect, antibacterial effect, chelating effect, surface activity and viscosity, and is suitable for encapsulating urine to perform abnormal analysis such as tangible objects in urine. If the concentration is too high or too low, the efficiency of these comprehensive effects will be reduced.

[0017] In a second aspect, the present application also proposes a method for preparing a sheath fluid for urine analysis, and adopts the following technical solution.

[0018] A method for preparing a sheath fluid for urine analysis as described above, by adjusting the concentrations of the 2-carbamoyl-3-nitrobenzoic acid and the 2-amino-1,3-propanediol hydrochloride, so that the viscosity of the sheath fluid is between 20 and 30 cps at 25°C, the pH is between 7.2 and 7.6, and the absorbance for 550nm light waves is between 0.8 and 1.2.

[0019] By adopting the above technical solution, the viscosity of the sheath liquid is between 20 and 30 cps at 25°C, and the viscosity of urine is generally 1 to 7 cps. The sheath liquid can wrap the urine during the test, so that the urine flows along the middle of the pipe, maintains a straight line flow, and will not dissolve with the urine too quickly, which is convenient for observing tangible objects in the urine and improving the reliability of the test results. The pH of the sheath liquid is between 7.2 and 7.6, forming an environment suitable for the survival of tangible objects in urine and prolonging the observation time. The absorbance of the sheath liquid for 550nm light waves is between 0.8 and 1.2, which is helpful for detecting tangible components in urine and is more in line with the use requirements of urine analyzers.

[0020] As an improvement to the method for preparing the sheath fluid for urine analysis, the concentration of the 2-aminoformyl-3-nitrobenzoic acid is adjusted to 5-8 g / L, and the concentration of the 2-amino-1,3-propanediol hydrochloride is adjusted to 2-4.5 g / L, so that the viscosity of the sheath fluid is between 20 and 30 cps at 25°C.

[0021] By adopting the above technical solution, suitable concentrations of 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride are formulated so that the viscosity of the sheath fluid is between 20 and 30 cps at 25° C., and comprehensive analysis and selection are made with the concentrations required to achieve other effects.

[0022] As an improvement to the method for preparing the sheath fluid for urine analysis, the pH of the sheath fluid is adjusted to between 7.2 and 7.6 by adjusting the concentration of the 2-aminoformyl-3-nitrobenzoic acid to 6-8 and the concentration of the 2-amino-1,3-propanediol hydrochloride to 2.5-5.

[0023] By adopting the above technical scheme, the appropriate concentrations of 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride are formulated so that the pH of the sheath fluid is between 7.2 and 7.6, and a comprehensive analysis and selection are made with the concentrations required to achieve other effects.

[0024] As an improvement to the method for preparing the sheath fluid for urine analysis, the concentration of the 2-carbamoyl-3-nitrobenzoic acid is adjusted to 3 to 7, and the concentration of the 2-amino-1,3-propanediol hydrochloride is adjusted to 1 to 4, so that the absorbance of the sheath fluid for 550 nm light waves is between 0.8 and 1.2.

[0025] By adopting the above technical solution, the appropriate concentrations of 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride are formulated so that the absorbance of the sheath fluid for 550nm light waves is between 0.8 and 1.2, and a comprehensive analysis and selection are made with the concentrations required to achieve other effects.

[0026] As an improvement to the method for preparing the sheath fluid for urine analysis, the total concentration of the osmotic pressure regulator is adjusted to 5 to 8 g / L so that the osmotic pressure of the sheath fluid is between 280 and 310 mOsm / (kg·H2O).

[0027] By adopting the above technical solution, the osmotic pressure of the sheath fluid is closer to the osmotic pressure of plasma, thereby maintaining the osmotic pressure environment of tangible objects such as red blood cells in urine, reducing the probability of water loss or swelling of tangible objects such as red blood cells, and maintaining activity for easy observation and analysis.

[0028] In summary, the sheath fluid for urine analysis and its preparation method of the present application have the following beneficial effects: 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride are rich in carboxyl groups, amino groups and other groups on the propane chain, which can balance pH, prevent the growth of bacteria, chelate osmotic pressure regulators, etc., so that it is not easy to precipitate and adhere to the urine analyzer. By adjusting the concentration of 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride, the appropriate absorbance and viscosity are adjusted, so that the sheath fluid can wrap urine during testing, so that urine flows along the middle of the pipe, and will not dissolve with urine too quickly, which is convenient for observing tangible objects in urine and improving the reliability of test results. 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride also have a certain defoaming effect, reducing the probability of bubbles in the sheath fluid.

[0029] When in use, first introduce a large amount of sheath liquid flow into the urine analyzer, then inject a small amount of urine into the middle of the sheath liquid flow, forming a core-shell liquid flow in which the sheath liquid wraps the urine, and then spread the liquid flow plane through the flat liquid flow channel, so that the formed components in the urine can be clearly seen from the front, so as to analyze the formed components in the urine. Due to the protection of the sheath flow, the sample will pass through the counting tube or hole in the center of the sheath flow, so there will be no biased interference signal, and each tangible object can be guaranteed to be free. The detection process does not require centrifugation and staining, the detection results are highly credible, and the detection cost is reduced. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the present application will be described in a complete and clear manner below. It is obvious that the implementation cases described are only part of the embodiments of the present invention, not all of the embodiments.

[0031] Example 1

[0032] 2-Carbamoyl-3-nitrobenzoic acid, sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0033] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid is 3 g / L, the concentration of sodium chloride is 4 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 0.5 g / L.

[0034] Example 2

[0035] 2-Carbamoyl-3-nitrobenzoic acid, sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0036] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid is 10 g / L, the concentration of sodium chloride is 9 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 6 g / L.

[0037] Example 3

[0038] 2-Carbamoyl-3-nitrobenzoic acid, sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0039] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid is 5 g / L, the concentration of sodium chloride is 5 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 2 g / L.

[0040] Example 4

[0041] 2-Carbamoyl-3-nitrobenzoic acid, sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0042] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid is 8 g / L, the concentration of sodium chloride is 8 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 4.5 g / L.

[0043] Example 5

[0044] 2-Carbamoyl-3-nitrobenzoic acid, sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0045] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid is 6 g / L, the concentration of sodium chloride is 6 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 2.5 g / L.

[0046] Example 6

[0047] 2-Carbamoyl-3-nitrobenzoic acid, sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0048] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid is 7 g / L, the concentration of sodium chloride is 7 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 4 g / L.

[0049] Comparative Example 1

[0050] Sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0051] In the prepared sheath liquid, the concentration of sodium chloride is 7 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 4 g / L.

[0052] Comparative Example 2

[0053] 2-Carbamoyl-3-nitrobenzoic acid and sodium chloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0054] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid was 7 g / L, and the concentration of sodium chloride was 7 g / L.

[0055] Comparative Example 3

[0056] 2-Carbamoyl-3-nitrobenzoic acid, sodium chloride and 2-amino-1,3-propanediol hydrochloride are added to water and mixed to obtain a sheath fluid for urine analysis.

[0057] In the prepared sheath liquid, the concentration of 2-carbamoyl-3-nitrobenzoic acid is 1 g / L, the concentration of sodium chloride is 1 g / L, and the concentration of 2-amino-1,3-propanediol hydrochloride is 10 g / L.

[0058] The formulas of the sheath liquids prepared in Examples 1-6 and Comparative Examples 1-3 are summarized in Table 1 below.

[0059] Table 1 Proportions of sheath fluids prepared in Examples 1-6 and Comparative Examples 1-3

[0060]

[0061] Test Example 1

[0062] The viscosity, pH, absorbance at 550 nm, osmotic pressure, etc. of the sheath liquids prepared in Examples 1-6 and Comparative Examples 1-3 are shown in Table 2 below.

[0063] Table 2 Properties of Sheath Liquids Prepared by Examples 1-6 and Comparative Examples 1-3

[0064]

[0065]

[0066] Based on the results in Table 1, since the viscosity of the sheath fluid is between 20 and 30 cps at 25°C, it can better wrap urine and is not easily miscible with urine when flowing. The pH of the sheath fluid is between 7.2 and 7.6, the osmotic pressure of the sheath fluid is between 280 and 310 mOsm / (kg·H2O), and the absorbance of the sheath fluid for 550nm light waves is between 0.8 and 1.2. It can better maintain the activity and morphology of red blood cells, proteins, etc. in urine, facilitate observation, and improve the reliability of the test results. Therefore, selecting the ratio of Examples 3-6 can achieve better comprehensive effects, especially the ratio of Examples 5-6 is better.

[0067] Test Example 2

[0068] 100 μl of the sheath fluid prepared in Examples 1-6 and Comparative Examples 1-3 were applied to the surface of the culture dish. Ten groups of 100 μl of the sheath fluid with a concentration of 1.2×10 6 cfu / ml of Staphylococcus aureus liquid were coated on the surface of the nine sheath liquid culture dishes and one sheath liquid-free culture dish, respectively, and cultured at 37°C for 6 hours. The colony counts on the surfaces of the ten culture dishes were compared, and the antibacterial rates of the sheath liquids prepared in Examples 1-6 were all above 99%, the antibacterial rate of the sheath liquid prepared in Comparative Example 1 was 62%, the antibacterial rate of the sheath liquid prepared in Comparative Example 2 was 75%, and the antibacterial rate of the sheath liquid prepared in Comparative Example 3 was 67%. The sheath liquids prepared in Examples 1-6 have good antibacterial effects, while the antibacterial rate of the sheath liquids prepared in Comparative Examples 1-3 is not ideal, because the amino groups of 2-carbamoyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride can also adhere to the surface of the bacteria, jointly preventing the bacteria from absorbing nutrients, and the nitro group can destroy the cell membrane, thereby inhibiting the growth of the bacteria, so that the sheath liquid meets the requirements of bacteria control.

[0069] Test Example 3

[0070] The sheath fluids prepared in Examples 1-6 and Comparative Examples 1-3 were respectively used in a urine analyzer for 5 days. No salt precipitation was observed in the urine analyzer in Examples 1-6 and Comparative Examples 2-3, while salt precipitation was observed in Comparative Example 1, indicating that 2-carbamoyl-3-nitrobenzoic acid has the effect of chelating and inhibiting salt precipitation. The main reason why the sheath fluids prepared in Examples 1-6 can inhibit salt precipitation is that the adjacent carboxyl, carbamoyl and nitro groups on the 2-carbamoyl-3-nitrobenzoic acid in the sheath fluid can also chelate the osmotic pressure regulator, etc., making it difficult to precipitate and adhere to the urine analyzer. During the application process, the sheath fluids of Examples 1-6 also did not have obvious bubbles, indicating that the sheath fluids prepared in Examples 1-6 have the effect of inhibiting salt precipitation and defoaming effect, while the sheath fluids prepared in Comparative Examples 1-3 have slight bubbles during use. The reason why the sheath fluid prepared in Example 1-6 has a defoaming effect is that 2-carbamoyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride also have a certain defoaming effect, reducing the probability of bubbles generated in the sheath fluid.

[0071] Through the above experiments, it can be seen that the sheath liquid for urine analysis of the present application has simple ingredients and stable properties. The combination of 2-aminoformyl-3-nitrobenzoic acid and 2-amino-1,3-propanediol hydrochloride enables the sheath liquid to have good antibacterial, defoaming, and salt precipitation prevention effects. When applied to urine detection, the sheath liquid can wrap urine so that the urine is in the middle of the pipe, does not dissolve with urine too quickly, maintains mutual independence during the flow process, and facilitates the detection of tangible objects in urine. When the concentration of the 2-aminoformyl-3-nitrobenzoic acid is 6-7g / L and the concentration of the 2-amino-1,3-propanediol hydrochloride is 2.5-4g / L, the sheath liquid has good antibacterial effect, urine wrapping and pushing effect, can inhibit salt precipitation, has an absorbance suitable for the urine analyzer, and is suitable for the pH of urine, so that the detection results of urine tangible objects are reliable and the detection cost is low.

[0072] The above is only a preferred embodiment of the present application, and the protection scope of the present application is not limited to the above embodiment. All technical solutions under the concept of the present application belong to the protection scope of the present application. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present application should also be regarded as falling within the protection scope of the present application.

Claims

1. A sheath fluid for urine analysis, characterized in that The sheath fluid includes water, 2-aminoformyl-3-nitrobenzoic acid, an osmotic pressure adjuster and 2-amino-1,3-propanediol hydrochloride; the concentration of the 2-aminoformyl-3-nitrobenzoic acid is 3-10 g / L; the concentration of the 2-amino-1,3-propanediol hydrochloride is 0.5-6 g / L.

2. The sheath fluid for urine analysis according to claim 1, characterized in that The osmotic pressure adjuster is sodium chloride and / or potassium chloride, and the total concentration of the osmotic pressure adjuster is 4-9 g / L.

3. A method for preparing a sheath fluid for urine analysis according to claim 1 or 2, characterized in that: By adjusting the concentrations of the 2-carbamoyl-3-nitrobenzoic acid and the 2-amino-1,3-propanediol hydrochloride, the viscosity of the sheath fluid is between 20 and 30 cps at 25° C., the pH is between 7.2 and 7.6, and the absorbance at 550 nm is between 0.8 and 1.

2.

4. The method for preparing a sheath fluid for urine analysis according to claim 3, characterized in that: The concentration of the 2-carbamoyl-3-nitrobenzoic acid is adjusted to 5-8 g / L, and the concentration of the 2-amino-1,3-propanediol hydrochloride is adjusted to 2-4.5 g / L, so that the viscosity of the sheath fluid is between 20-30 cps at 25° C.

5. The method for preparing a sheath fluid for urine analysis according to claim 3, characterized in that: The pH of the sheath fluid is adjusted to between 7.2 and 7.6 by adjusting the concentration of the 2-aminoformyl-3-nitrobenzoic acid to 6-8 g / L and the concentration of the 2-amino-1,3-propanediol hydrochloride to 2.5-5 g / L.

6. The method for preparing a sheath fluid for urine analysis according to claim 3, characterized in that: By adjusting the concentration of the 2-carbamoyl-3-nitrobenzoic acid to 3-7 g / L and the concentration of the 2-amino-1,3-propanediol hydrochloride to 1-4 g / L, the absorbance of the sheath fluid for 550 nm light waves is between 0.8 and 1.

2.

7. The method for preparing a sheath fluid for urine analysis according to claim 3, characterized in that: The total concentration of the osmotic pressure adjusting agent is adjusted to 5-8 g / L, so that the osmotic pressure of the sheath fluid is between 280-310 mOsm / (kg·H2O).

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