A biostimulant composition for increasing the folic acid content of crops and a preparation and use method thereof

Abstract

The present invention provides a biostimulant composition for increasing the folate content of crops, including an aqueous solution of synthetic folic acid and L-potassium glutamate, wherein the molar concentration ratio of the synthetic folic acid to L-potassium glutamate is 1:3-5, and the solvent is an aqueous solution of potassium carbonate. The present invention also provides a preparation and use method of the biostimulant composition. Synthetic folic acid is used as the main body of plant biostimulants, and L-glutamic acid, one of the synthetic precursors of natural folic acid, is added to stimulate the main body of crops such as fruits and vegetables to increase the synthesis and storage of natural folic acid, thereby increasing the content of folic acid in crops and improving the quality of crops.

Description

Technical Field

[0001] The invention belongs to the technical field of plant production safety, and in particular relates to a biostimulant composition for increasing the folic acid content of crops and a preparation and use method thereof. Background Art

[0002] Folic acid is a type of water-soluble B vitamin, with a chemical name of pteroylglutamate. It is an essential micronutrient for the growth and development of plants and animals, and its deficiency increases the risk of many diseases. Plants and microorganisms can synthesize folic acid during growth to meet their own needs, but humans and animals cannot synthesize folic acid and can only obtain it through diet. Among edible plants, except for spinach, beans and cabbage, which have relatively high folic acid content, the folic acid content of other foods such as grain crops, vegetables and fruits is very low, far from meeting the human body's demand for folic acid. At present, people's insufficient intake of folic acid is mainly supplemented by eating industrially synthesized folic acid.

[0003] Folic acid is not easily soluble in water at room temperature, and its solubility in water at 25°C is only 0.0016g / L, but it is easily soluble in carbonate-alkali solution. Folic acid itself is inactive. After being reduced to tetrahydrofolate in the body, it becomes an important coenzyme for one-carbon unit transfer reactions such as methyl, methylene, formyl and hydroxymethyl, and plays a key role in the biosynthesis of purine, thymine, DNA, amino acids, proteins and chlorophyll, as well as in the methyl cycle. In addition, folic acid is also involved in the biosynthesis of chlorophyll and the antioxidant stress of fruits. For example, the folic acid coenzyme in photolyase and cryptochrome in plants can capture light energy and play an important role in the photosynthesis of plants. Natural folic acid, as a type of organic substance produced by the metabolism of plants themselves, has obvious physiological effects on plants at extremely low concentrations, and has the function of stimulating and strengthening the natural physiological processes in plants. When folic acid is deficient, the methyl cycle, the synthesis of DNA and proteins, and gene expression will be impaired, and photosynthesis will be inactive.

[0004] The parent compound of folic acid is composed of three parts: pteridine, para-aminobenzoic acid (PABA) and glutamate. In recent years, the method of increasing the folic acid content in plants is mainly achieved by overexpressing key enzymes in the folic acid synthesis pathway through transgenic technology. For example, by simultaneously overexpressing GTP cyclohydrolase (GTPCHI) in the pteridine synthesis process and aminodeoxychorismate synthase (ADCS) in the para-aminobenzoic acid (PABA) synthesis branch, the folic acid content in some plants has been significantly increased. However, due to the differences in gene coding in cells of different species, transgenic operations through overexpression of key enzymes are only achieved in some plants, which is not universal for a wide variety of crops, limiting the application of this type of technology in agricultural production.

[0005] The structure of industrially synthesized folic acid is pteroylmonoglutamic acid, while the structure of natural folic acid is pteroylpolyglutamic acid. The length of the glutamic acid chain varies with the cells or organelles where the folic acid is located, and can be up to 6 to 9 in length. In most eukaryotic cells, folic acid with a glutamic acid polymerization degree of 5 is the most common. Folic acid in plants is mainly transported and stored in the form of pteroylpolyglutamic acid. A large part of its physiological activity is regulated by the length of the glutamic acid chain. In the enzymatic reaction of C1 metabolism, folic acid with polyglutamic acid chains has a stronger ionicity and a more active reaction. Natural folic acid contained in green leafy vegetables is absorbed in the human intestine, while synthetic folic acid is absorbed in the liver. The amount of synthetic folic acid absorbed by the liver is limited, and the excess synthetic folic acid that is not absorbed will enter the blood, which may cause diseases such as leukemia and arthritis. At present, eating foods with high natural folate content is a safe choice to solve the problem of insufficient folate intake among the population. However, there has been no public report on the technical solution of how to convert chemically synthesized folate into natural folate through the plant folate synthesis pathway, thereby increasing the folate content in crops and regulating the growth of crops. Summary of the invention

[0006] In order to solve the problems in the prior art, the present invention provides a biostimulant composition for increasing the folate content of crops, as well as a preparation and use method thereof. Synthetic folic acid is used as the main body of the plant biostimulant, and L-glutamic acid, one of the synthetic precursors of natural folic acid, is added to stimulate the main body of crops such as fruits and vegetables to increase the synthesis and storage of natural folic acid, thereby increasing the folate content in crops.

[0007] The present invention solves the technical problem by adopting the following technical solutions:

[0008] The first object of the present invention is to provide a biostimulant composition for increasing the folate content of crops, characterized in that it comprises an aqueous solution of synthetic folic acid and potassium L-glutamate, wherein the molar concentration ratio of the synthetic folic acid to potassium L-glutamate is 1:3-5, and the solvent is an aqueous solution of potassium carbonate.

[0009] Furthermore, the concentration of the synthetic folic acid is 200 g / L, and the concentration of potassium L-glutamate is 233-389 g / L.

[0010] The second object of the present invention is to provide a method for preparing a biostimulant composition with increased folic acid content, characterized in that it comprises the following steps:

[0011] Weigh synthetic folic acid according to a proportion and put it into an alkali dissolving tank, add purified water to mix and stir, add potassium carbonate solution, continue to stir and dissolve, and make the pH value of the solution between 7 and 7.5; add weighed potassium L-glutamate to the stirred solution, and continue to stir and dissolve; after dissolution, add purified water to make the volume to the target capacity to obtain the biostimulant composition.

[0012] Furthermore, the mass ratio of the added potassium carbonate to the synthetic folic acid is 3:8.

[0013] Furthermore, the concentration of the added potassium carbonate solution is 150 g / L.

[0014] Furthermore, based on the plant stimulant combination per liter, the concentration of synthetic folic acid in the biostimulant composition is 200 g / L, and the concentration of potassium L-glutamate is 233-389 g / L.

[0015] The third object of the present invention is to provide a method for using a biostimulant composition for increasing the folic acid content of crops, characterized in that the biostimulant composition obtained by the above preparation method is sprayed on the leaves of crops for 1 to 3 rounds during their growth.

[0016] Furthermore, when spraying on leaves, the biostimulant composition needs to be diluted to a folic acid concentration of 0.2-0.8 g / L, and the amount of the diluted biostimulant composition sprayed per mu is 25-35 L.

[0017] Furthermore, there should be at least a one-week interval between each round of foliar spraying.

[0018] Furthermore, foliar spraying is carried out during the budding stage, fruit setting stage, fruit expansion stage, or filling stage.

[0019] Furthermore, it can be used alone or mixed with medium element fertilizer, trace element fertilizer, and amino acid water-soluble fertilizer for foliar spraying.

[0020] It should be noted that the present invention is applicable to various crops, including tea, vegetables, fruits, staple food and other plants, such as leaves, rhizomes, fruits, beans and the like.

[0021] The plant biostimulant combination of the present invention creatively adds synthetic folic acid and potassium L-glutamate. After the plant biostimulant combination is absorbed by crops, a glutamic acid tail is added to monoglutamic acid folic acid through the catalytic action of polyglutamic acid folate synthase (FPGS) in the mitochondria, and the synthetic folic acid is converted into polyglutamic acid folic acid (natural plant folic acid). The mechanism that polyglutamic acid folic acid can be transported between subcellular organelles without obstacles is utilized to increase the transportation and storage of folic acid to the cytoplasm, vacuole and chloroplast, and exert a regulatory effect on plant growth. Because the vacuole does not contain enzymes related to folic acid synthesis, folic acid can be accumulated in large quantities therein without generating feedback inhibition, thereby greatly increasing the folic acid content in crops.

[0022] In the present invention, potassium carbonate aqueous solution is used as an alkaline solvent, which greatly improves the solubility of synthetic folic acid in water at room temperature, laying a foundation for synthetic folic acid to be absorbed and converted by crops as one of the synthetic precursors of natural folic acid. At the same time, compared with synthetic folic acid, folate can pass through plant cell channels faster, increasing plant absorption. Potassium is an essential element for crop growth, participates in the activation of multiple enzymes, promotes multiple metabolic activities, and plays an important role in many functions of crops such as photosynthesis, enzyme activation and protein synthesis.

[0023]

[0024] The biostimulant combination of the present invention achieves the regulatory effect on the folate synthesis of crops in two aspects. First, the increase of precursor substances in the folate synthesis pathway in crops stimulates the synthesis and activity of related enzymes in the pathway, and the folate synthesis pathway becomes a high-speed channel for the photosynthesis synthesis of crops, exerting the plant biostimulant effect; second, the sufficient supply of folate in crops can promote the methyl cycle, DNA synthesis and gene expression, synthesis of folate-dependent amino acids, cell division and growth, chlorophyll synthesis and photosynthesis are all in an active state, thereby promoting the growth of crops and improving the quality of crops.

[0025] Compared with the prior art, the beneficial technical effects of the present invention are:

[0026] 1. The plant biostimulant combination of the present invention can significantly increase the folic acid content in crops, while stimulating the synthesis of folic acid-dependent amino acids, increasing the total amino acid and free amino acid content of harvested crop fruits, increasing the polysaccharide content of crops, improving the stress resistance of crops, and significantly improving the quality of crops.

[0027] 2. The plant biostimulant combination of the present invention has the characteristics of small dosage and high activity. After spraying, it can greatly increase the average single fruit weight and per mu yield of crops.

[0028] 3. The present invention can improve the absorption and utilization efficiency of nutrients by crops, promote plant growth, and enhance the resistance to abiotic stress and antioxidant properties of crops, and the efficacy is independent of its nutritional components.

[0029] 4. The raw materials used in the plant biostimulant combination of the present invention are moderately priced, easily available, and have a high output, which can improve the economic benefits of farmland at a lower investment cost.

[0030] 5. The plant biostimulant combination of the present invention can be stored and transported at room temperature, is simple to operate, and is easy to use and promote.

[0031] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention, it can be implemented according to the contents of the specification. In order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are listed below. DETAILED DESCRIPTION

[0032] The technical solution of the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only exemplary illustrations and explanations of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are included in the scope that the present invention is intended to protect.

[0033] In addition, unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0034] Example 1 Potato product application

[0035] The 1,200-mu potato functional agricultural experimental base was divided into 12 areas, each area was set with a 200-cm protection row, and 4 experimental groups 1, 4 experimental groups 2 and 4 control groups were set. After diluting the plant biostimulant composition for increasing the folate content of crops prepared by the preparation method of the present invention, the experimental group was sprayed with the diluent of the "plant biostimulant composition for increasing the folate content of crops", and the control group was sprayed with pure water. The fertilization, planting time, pesticides and daily field management of each area were the same.

[0036] In this embodiment, a method for preparing a biostimulant composition for increasing the folate content of crops comprises the following steps:

[0037] Weigh 20kg of synthetic folic acid, 7.5kg of potassium carbonate, and 23.3kg of potassium L-glutamate in sequence. First, add pure water to potassium carbonate to make a potassium carbonate solution, and the concentration of the prepared potassium carbonate solution is 150g / L. Put the weighed synthetic folic acid into a 100L alkali dissolving tank, add 10L of pure water to mix and stir, and slowly add the prepared potassium carbonate solution, continue to stir and dissolve, so that the pH value of the solution is between 7 and 7.5; add the weighed potassium L-glutamate to the stirred solution, and continue to stir and dissolve; after dissolution, add pure water to the target capacity of 100L to obtain a biostimulant composition.

[0038] Use of biostimulant composition:

[0039] During the fruit expansion period of potatoes, the prepared biostimulant composition was diluted 500 times and sprayed on the experimental group potatoes. The experimental group 1 was sprayed once, and the experimental group 2 was sprayed twice. The second round of foliar spraying was carried out one week after the first round of foliar spraying. When spraying on the leaves, the spraying amount per mu was 35L, and it was generally sprayed after 5 pm.

[0040] By observing the growth of potatoes in 12 zones, we found that: 1) the potatoes in the experimental group grew vigorously, with an average plant height 10 to 20 cm higher than that in the control group; 2) the leaves were large and flat, dark green in color, and thicker; 3) the plants had good disease resistance, enhanced resistance to abiotic stresses, slowed leaf aging, and extended the growth period by about 15 days.

[0041] After the potatoes were harvested, each group was sent to Shanghai SGS for testing. The data in each group were averaged, and the comparison of folic acid content and nutritional components was shown in Table 1.

[0042] Table 1 Comparison of nutritional components of potato experimental group and control group

[0043]

[0044] As can be seen from Table 1, the use of the biostimulant composition of the present application has greatly increased the folic acid content and nutrients such as reducing sugars compared to no use, especially the folic acid content, which has a large increase. Compared with two rounds of foliar spraying of the biostimulant composition of the present application, the folic acid content of the spraying of one round increased by 47.8% compared to the control group, and the folic acid content of the spraying of two rounds increased by 144% compared to the control group.

[0045] Example 2 Apple product application

[0046] 200 mu of apple functional agriculture pilot farmland was divided into 10 areas, each area was set with a 400 cm protection row, and 8 experimental groups and 2 control groups were randomly set. After diluting the plant biostimulant composition for increasing the folate content of crops prepared by the preparation method of the present invention, the experimental group was sprayed with the dilution of the "plant biostimulant combination for increasing the folate content of crops", and the control group was sprayed with pure water. The fertilization, planting time, pesticides and daily field management of each area were the same.

[0047] A method for preparing a biostimulant composition for increasing the folic acid content of crops comprises the following steps:

[0048] Weigh 20kg of synthetic folic acid, 7.5kg of potassium carbonate, and 38.9kg of potassium L-glutamate in sequence. First, add pure water to potassium carbonate to make a potassium carbonate solution, and the concentration of the prepared potassium carbonate solution is 150g / L. Put the weighed synthetic folic acid into a 100L alkali dissolving tank, add 10L of pure water to mix and stir, and slowly add the prepared potassium carbonate solution, continue to stir and dissolve, so that the pH value of the solution is between 7 and 7.5; add the weighed potassium L-glutamate to the stirred solution, and continue to stir and dissolve; after dissolution, add pure water to the target capacity of 100L to obtain a biostimulant composition.

[0049] Use of biostimulant composition:

[0050] During the fruit-setting period of apples, the prepared biostimulant composition was diluted 500 times and sprayed on the leaves of the apple trees in the experimental group for the first round. The spraying amount per mu in the first round was 25L.

[0051] During the sugar conversion and coloring period of apples, the prepared biostimulant composition was diluted 250 times and sprayed on the apple trees in the experimental group for the second round of foliage spraying. The spraying amount per mu in the second round was 25L. When spraying in two rounds, try to choose after 5 pm on cloudy or sunny days.

[0052] By observing the growth of apples in 10 sub-areas, it can be found that spraying the biostimulant composition of the present invention has a significant regulating effect on the fruit diameter and weight of apples. The leaves of the apple trees in the experimental group are oily green and darker in color. The number of apples with a fruit diameter of more than 85 in the experimental group increased by 20% compared with the control group.

[0053] After the apples were harvested, each group was sent to Shanghai SGS for testing. The data within each group were averaged, and the comparison of folic acid content is shown in Table 2.

[0054] Table 2 Comparison of folic acid detection between apple experimental group and control group

[0055]

[0056] As can be seen from Table 2, compared with not using the biostimulant composition of the present application, the folic acid content of apples after spraying two rounds of the biostimulant composition of the present application increased by 501.9% compared with the control group, the antioxidant performance was greatly improved, and there was no oxidative browning four hours after cutting.

[0057] Example 3 Application of tea products

[0058] The 300-mu tea experimental base was divided into 6 areas, each area was set with a 200-cm protection row, and 5 experimental groups and 1 control group were randomly set. After diluting the plant biostimulant composition for increasing the folate content of crops prepared by the preparation method of the present invention, the experimental group was sprayed with the diluent of the "plant biostimulant composition for increasing the folate content of crops", and the control group was sprayed with pure water. The fertilization, planting time, pesticides and daily field management of each area were the same.

[0059] The preparation method of the biostimulant composition for increasing the folate content of crops is the same as that in Example 1.

[0060] Use of biostimulant composition:

[0061] During the budding period of tea leaves, the prepared biostimulant composition was diluted 1000 times and sprayed on the leaves of the experimental group of tea trees for the first round. The amount of the diluted biostimulant composition sprayed per mu in the first round was 25 L.

[0062] One month before tea picking, the prepared biostimulant composition was diluted 500 times and sprayed on the tea trees in the experimental group for the second round of foliage spraying. The spraying volume per mu in the second round was 35L. The two rounds of spraying should be selected before 10 am or after 3 pm.

[0063] By observing the growth conditions of tea leaves in 6 zones, it can be found that after spraying the biostimulant composition of the present invention, the tea tree tops have obvious advantages, the branches grow vigorously, and the leaves are still bright green at the harvest time. Compared with the control group, the leaves are also darker and greener.

[0064] When the tea leaves are mature, they are harvested uniformly according to the one bud and two leaves method, and each group of tea leaves is sent to Shanghai SGS for testing. After taking the average value of the data in each group, the comparison of folic acid content and nutritional components is shown in Table 3.

[0065] Table 3 Comparison of folic acid and nutritional components between the tea experimental group and the control group

[0066]

[0067] As can be seen from Table 3, compared with not using the biostimulant composition of the present application, the folic acid content of the tea leaves after spraying two rounds of the biostimulant composition of the present application increased by 67.8%, the amino acid content increased by 10.5%, the tea polyphenol content increased by 26.1%, and the caffeine content increased by 20% compared with the control group. After spraying the biostimulant composition of the present invention, the beneficial components in the tea leaves, such as amino acids and tea polyphenols, were significantly improved, which not only increased the folic acid content, but also improved the quality of the tea leaves.

[0068] The serial numbers of the above embodiments of the present invention are only for description and do not represent the advantages or disadvantages of the embodiments.

[0069] The embodiments of the present invention are described above, but the present invention is not limited to the above-mentioned specific implementation modes. The above-mentioned specific implementation modes are merely illustrative and not restrictive. Under the guidance of the present invention, ordinary technicians in this field can also make many forms without departing from the scope of protection of the purpose of the present invention and the claims, which are all within the protection of the present invention.

Claims

1. A method for using a biostimulant composition for increasing the folic acid content of crops, characterized in that: The biostimulant composition is sprayed on the leaves for 1 to 3 rounds during the growth process. The biostimulant composition includes an aqueous solution of synthetic folic acid and potassium L-glutamate, the molar concentration ratio of the synthetic folic acid to potassium L-glutamate is 1:3-5, and the solvent is an aqueous solution of potassium carbonate. When sprayed on the leaves, the biostimulant composition needs to be diluted to a folic acid concentration of 0.2 to 0.8 g / L, and the amount of the diluted biostimulant composition sprayed per mu is 25 to 35 L.

2. The method for using the biostimulant composition for increasing the folate content of crops according to claim 1, characterized in that: The preparation method of the biostimulant composition comprises the following steps: Weigh synthetic folic acid according to a proportion and put it into an alkali dissolving tank, add purified water and mix and stir, add potassium carbonate solution, continue to stir and dissolve, and make the pH value of the solution between 7 and 7.5; add weighed potassium L-glutamate to the obtained solution, and continue to stir and dissolve; after dissolution, add purified water to make the volume to the target capacity to obtain the biostimulant composition.

3. The method for using the biostimulant composition for increasing the folic acid content of crops as claimed in claim 1, characterized in that: The concentration of the synthetic folic acid is 200 g / L, and the concentration of potassium L-glutamate is 233-389 g / L.

4. The method for using the biostimulant composition for increasing the folate content of crops as claimed in claim 1, characterized in that: The mass ratio of the added potassium carbonate to the synthetic folic acid is 3:

8.

5. The method for using the biostimulant composition for increasing the folate content of crops as claimed in claim 1, characterized in that: The concentration of the added potassium carbonate solution was 150 g / L.

6. The method for using the biostimulant composition for increasing the folic acid content of crops as claimed in claim 1, characterized in that: Based on per liter of the plant stimulant combination, the concentration of synthetic folic acid in the biostimulant composition is 200 g / L, and the concentration of potassium L-glutamate is 233-389 g / L.

7. The method for using the biostimulant composition for increasing the folate content of crops as claimed in claim 1, characterized in that: It can be used alone for foliar spraying, or mixed with medium element fertilizer, trace element fertilizer, and amino acid water-soluble fertilizer.

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