Complex enzyme and method for using the same in manufacturing water-soluble fertilizer

Abstract

The application discloses a kind of composite enzyme, cellulase, xylosolase, papain are composed according to mass ratio 2-4:1-5:0.5-0.7.The use method of the composite enzyme in the manufacture of water-soluble fertilizer, comprising the following steps: (1) soybean is made into soybean meal powder, standby;(2) after mixing raw materials and stirring uniformly, fermentation enzymolysis is carried out, and fermentation enzymolysis liquid is prepared;(3) the fermentation enzymolysis liquid prepared in step (2) is filtered using filter tank and then placed in filter press for solid-liquid separation, and filtrate is prepared;(4) the filtrate prepared in step (3) is placed in inactivation device for inactivation treatment, and water-soluble fertilizer is prepared.The water-soluble fertilizer product produced by the application contains rich plant-derived amino acid small peptides, polypeptides, oligosaccharides and other active elements and various chelated trace elements, no waste residue is generated during preparation, no emissions, low energy consumption, simple and easy-to-control production process, easy to form scale production, which is beneficial to cost reduction and efficiency improvement.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of water-soluble fertilizer, and particularly relates to a compound enzyme and a use method of the compound enzyme in manufacturing water-soluble fertilizer. BACKGROUND

[0002] The water-soluble fertilizer refers to a compound fertilizer capable of completely dissolving in water and containing nitrogen, phosphorus, potassium, calcium, magnesium, trace elements, amino acids, humic acid, alginic acid and the like. The water-soluble fertilizer has strict standards for raw material quality and processing technology. Researches show that the use of the water-soluble fertilizer can improve the quality of fruits and vegetables.

[0003] The water-soluble fertilizer has a great boosting effect on the development of modern agriculture in China, but the existing water-soluble fertilizer still has some technical problems, such as a complex production process, a long production cycle, a large amount of by-products polluting the environment, a low content of organic matter and free amino acid, a low product activity, and a poor improvement effect on the current soil hardening, etc. Therefore, how to develop a water-soluble fertilizer with a short production cycle and high quality has become a new research direction. SUMMARY

[0004] The application provides a compound enzyme and a use method of the compound enzyme in manufacturing water-soluble fertilizer, so as to solve the problems of a long production cycle, a low product activity and a poor soil improvement effect of the water-soluble fertilizer prepared by the prior art.

[0005] To solve the above technical problems, the application adopts the following technical scheme:

[0006] The compound enzyme is composed of cellulase, xylosidase and papain at a mass ratio of 2-4:1-5:0.5-0.7.

[0007] Preferably, the compound enzyme is composed of cellulase, xylosidase and papain at a mass ratio of 3:4:0.6.

[0008] The application further provides a use method of the compound enzyme in manufacturing water-soluble fertilizer, which comprises the following steps:

[0009] (1) soybeans are prepared into soybean meal powder for standby;

[0010] (2) the soybean meal powder, algal extract, organic nitrogen, phosphorus and potassium source, mannitol, glucose, algal polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, compound enzyme, modifier and water are uniformly mixed and stirred to perform fermentation and enzymolysis, so as to prepare a fermentation and enzymolysis liquid;

[0011] (3) the fermentation and enzymolysis liquid prepared in the step (2) is filtered by using a filter tank and then is placed into a filter press to perform solid-liquid separation, so as to prepare a filtrate;

[0012] (4) the filtrate prepared in the step (3) is placed into an inactivation device to perform inactivation treatment, so as to prepare a water-soluble fertilizer.

[0013] Preferably, the raw materials in step (2) are mixed and stirred uniformly in the following weight parts: soybean meal powder 60-70 parts, seaweed extract 10-15 parts, organic nitrogen phosphorus potassium source 5-7 parts, mannitol 4-8 parts, glucose 2-5 parts, seaweed polysaccharide 4-8 parts, polyglutamic acid 2-4 parts, salicylic acid 0.04-0.08 parts, fermentation bacteria 0.3-0.5 parts, complex enzyme 0.1-0.2 parts, modifier 0.2-0.8 parts, and water 120-200 parts.

[0014] Preferably, the organic nitrogen phosphorus potassium source is brown sugar.

[0015] Preferably, the modifier is made of polyacrylamide, hydroxyapatite, and humic acid in a mass ratio of 0.5-1:2-5:2-3.

[0016] More preferably, the modifier is made of polyacrylamide, hydroxyapatite, and humic acid in a mass ratio of 0.8:4:2.7.

[0017] Preferably, the fermentation bacteria are composed of yeast, lactic acid bacteria, and bacillus licheniformis in a mass ratio of 4-8:0.3-0.6:1-2.

[0018] More preferably, the fermentation bacteria are composed of yeast, lactic acid bacteria, and bacillus licheniformis in a mass ratio of 6:0.5:1.3.

[0019] Preferably, the fermentation and enzymolysis in step (2) are carried out at a temperature of 25-32℃ for 5-8 days.

[0020] The present application has the following beneficial effects:

[0021] (1) The complex enzyme used in the present application is composed of cellulase, xylitolase, and papain, and the proportion is controlled, and the three enzymes cooperate with each other to effectively accelerate the enzymolysis of the raw materials, which can be completed in 5-8 days, greatly shortening the production cycle.

[0022] (2) The improver of the application can effectively improve soil structure, increase soil porosity and capillary porosity, increase the number of soil large aggregates, and reduce soil bulk density. It is beneficial to the formation of soil large aggregates, the improvement of soil structure, the promotion of soil water infiltration and the increase of water content, the increase of soil fertilizer utilization rate, the improvement of soil fertility and the increase of yield. It can effectively prevent soil erosion, and has no effect on microorganisms in the soil, ensuring the planting efficiency of the soil; hydroxyapatite contains a large amount of calcium ions, and its structure contains pores and a large specific surface area. The heavy metals in the soil combine with OH- to form hydroxyl compounds, providing more adsorption sites for heavy metal ions, which can adsorb heavy metal ions in the soil and adjust the pH of the soil; humic acid interacts with calcium ions in the soil to form flocculent precipitated gel. The soil is cemented together to form water-stable aggregate structure, which can maintain the permeability, absorbability and retention of water and air, thereby meeting the needs of plant growth for water, oxygen and air. It can also form complexes with iron and aluminum in the soil, thereby reducing the toxicity of these acidic oxides to crops and buffering the alkaline substances.

[0023] (3) The hydroxyapatite of the application can carry polyacrylamide due to its large specific surface area, increase the infiltration depth of polyacrylamide into the soil, and the presence of calcium ions can increase the viscosity of the polyacrylamide aqueous solution, improve the rate of polyacrylamide infiltration into the soil, and improve the aggregation effect of polyacrylamide on the soil; the polyacrylamide aqueous solution can fix heavy metals in the soil, which is beneficial to the exchange and adsorption of hydroxyapatite on heavy metal ions, increasing the removal of hydroxyapatite on heavy metal ions; humic acid can improve the porosity of the soil, which is beneficial to increasing the infiltration depth of hydroxyapatite carrying polyacrylamide into the soil, increasing the effect, and at the same time, increasing the water retention performance of the soil, which is beneficial to the viscosity of the polyacrylamide aqueous solution, further improving the infiltration rate of polyacrylamide.

[0024] (4) The water-soluble fertilizer product produced by the application contains rich active elements such as plant-derived amino acid small peptides, polypeptides and oligosaccharides, as well as various chelated trace elements. The preparation process does not produce waste residue, has no emissions, low energy consumption, simple and easy-to-control production process, easy to form large-scale production, and is conducive to cost reduction and efficiency improvement.

[0025] (5) The water-soluble fertilizer prepared by the application has excellent technical indicators, including organic matter content ≥200g / L; free amino acid content ≥80g / L; nitrogen, phosphorus and potassium ≥100g / L; aluminum, zinc, boron, iron, calcium and magnesium >30g / L; and water-insoluble substance <20g / L, which can meet the growth needs of crops.

DETAILED DESCRIPTION

[0026] For better understanding of the present application, the following examples are illustrated, which belong to the protection scope of the present application, but do not limit the protection scope of the present application.

[0027] A water-soluble fertilizer is made from raw materials including the following parts by weight: soybean meal powder 60-70 parts, seaweed extract 10-15 parts, organic nitrogen phosphorus potassium source 5-7 parts, mannitol 4-8 parts, glucose 2-5 parts, algal polysaccharide 4-8 parts, polyglutamic acid 2-4 parts, salicylic acid 0.04-0.08 parts, fermentation bacteria 0.3-0.5 parts, composite enzyme 0.1-0.2 parts, modifier 0.2-0.8 parts, and water 120-200 parts.

[0028] The organic nitrogen phosphorus potassium source is brown sugar.

[0029] The composite enzyme is composed of cellulase, xylitolase, and papain in a mass ratio of 2-4:1-5:0.5-0.7.

[0030] The modifier is made from polyacrylamide, hydroxyapatite, and humic acid in a mass ratio of 0.5-1:2-5:2-3.

[0031] The fermentation bacteria are composed of yeast, lactic acid bacteria, and bacillus licheniformis in a mass ratio of 4-8:0.3-0.6:1-2.

[0032] The use method of the composite enzyme in the manufacture of water-soluble fertilizer includes the following steps:

[0033] (1) soybeans are made into soybean meal powder for standby;

[0034] (2) the soybean meal powder, seaweed extract, organic nitrogen phosphorus potassium source, mannitol, glucose, algal polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, composite enzyme, modifier, and water are mixed and stirred uniformly, and then subjected to fermentation and enzymatic hydrolysis at a temperature of 25-32℃ for 5-8 days to obtain a fermentation and enzymatic hydrolysis liquid;

[0035] (3) the fermentation and enzymatic hydrolysis liquid obtained in step (2) is filtered by using a filter tank and then subjected to solid-liquid separation in a filter press to obtain a filtrate;

[0036] (4) the filtrate obtained in step (3) is subjected to inactivation treatment in an inactivation device to obtain a water-soluble fertilizer.

[0037] The following is illustrated by more specific examples.

[0038] Example 1

[0039] A water-soluble fertilizer is made from raw materials including soybean meal powder 70 parts, seaweed extract 10 parts, organic nitrogen, phosphorus and potassium source 6 parts, mannitol 8 parts, glucose 3 parts, seaweed polysaccharide 5 parts, polyglutamic acid 4 parts, salicylic acid 0.05 parts, fermentation bacteria 0.4 parts, composite enzyme 0.2 parts, modifier 0.6 parts, and water 180 parts.

[0040] The organic nitrogen, phosphorus and potassium source is brown sugar.

[0041] The composite enzyme is composed of cellulase, xylosidase and papain at a mass ratio of 4:2:0.5.

[0042] The modifier is made from polyacrylamide, hydroxyapatite and humic acid at a mass ratio of 0.8:3:2.

[0043] The fermentation bacteria are composed of yeast, lactic acid bacteria and bacillus licheniformis at a mass ratio of 5:0.3:1.

[0044] The use of the composite enzyme in the manufacture of water-soluble fertilizer includes the following steps:

[0045] (1) soybean is made into soybean meal powder for standby;

[0046] (2) the soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium source, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, composite enzyme, modifier and water are mixed and stirred uniformly, then fermented and enzymatically hydrolyzed at a temperature of 26℃ for 8 days to obtain a fermentation and enzymatic hydrolysis liquid;

[0047] (3) the fermentation and enzymatic hydrolysis liquid obtained in step (2) is filtered by a filter tank and then subjected to solid-liquid separation in a filter press to obtain a filtrate;

[0048] (4) the filtrate obtained in step (3) is subjected to inactivation treatment in an inactivation device to obtain a water-soluble fertilizer.

[0049] Example 2

[0050] A water-soluble fertilizer is made from raw materials including soybean meal powder 60 parts, seaweed extract 12 parts, organic nitrogen, phosphorus and potassium source 7 parts, mannitol 4 parts, glucose 2 parts, seaweed polysaccharide 4 parts, polyglutamic acid 2 parts, salicylic acid 0.04 parts, fermentation bacteria 0.5 parts, composite enzyme 0.1 parts, modifier 0.2 parts, and water 150 parts.

[0051] The organic nitrogen, phosphorus and potassium source is brown sugar.

[0052] The composite enzyme is composed of cellulase, xylosidase and papain at a mass ratio of 2:2:0.7.

[0053] The modifier is made from polyacrylamide, hydroxyapatite and humic acid at a mass ratio of 0.5:5:3.

[0054] The fermentation bacteria are composed of yeast, lactic acid bacteria and bacillus licheniformis at a mass ratio of 4:0.5:2.

[0055] The method for using the compound enzyme in the preparation of water-soluble fertilizer comprises the following steps:

[0056] (1) soybeans are prepared into soybean meal powder for standby;

[0057] (2) the soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium source, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, compound enzyme, modifier and water are uniformly mixed and stirred, and then subjected to fermentation and enzymolysis at a temperature of 25 DEG C for 8 days to prepare fermentation and enzymolysis liquid;

[0058] (3) the fermentation and enzymolysis liquid prepared in step (2) is filtered by using a filter tank and then subjected to solid-liquid separation in a filter press to prepare filtrate;

[0059] (4) the filtrate prepared in step (3) is subjected to inactivation treatment in an inactivation device to prepare water-soluble fertilizer.

[0060] Example 3

[0061] A kind of water-soluble fertilizer, which is prepared from the following raw materials by weight: soybean meal powder 60 parts, seaweed extract 15 parts, organic nitrogen, phosphorus and potassium source 5 parts, mannitol 6 parts, glucose 5 parts, seaweed polysaccharide 6 parts, polyglutamic acid 2 parts, salicylic acid 0.08 parts, fermentation bacteria 0.5 parts, compound enzyme 0.1 parts, modifier 0.4 parts, and water 200 parts.

[0062] The organic nitrogen, phosphorus and potassium source is brown sugar.

[0063] The compound enzyme is composed of cellulase, xylitolase and papain at a mass ratio of 3:4:0.6.

[0064] The modifier is prepared from polyacrylamide, hydroxyapatite and humic acid at a mass ratio of 0.8:4:2.7.

[0065] The fermentation bacteria are composed of yeast, lactic acid bacteria and bacillus licheniformis at a mass ratio of 6:0.5:1.3.

[0066] The method for using the compound enzyme in the preparation of water-soluble fertilizer comprises the following steps:

[0067] (1) soybeans are prepared into soybean meal powder for standby;

[0068] (2) the soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium source, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, compound enzyme, modifier and water are uniformly mixed and stirred, and then subjected to fermentation and enzymolysis at a temperature of 32 DEG C for 5 days to prepare fermentation and enzymolysis liquid;

[0069] (3) using a filter tank to filter the fermentation enzymolysis solution prepared in step (2) and then placing it in a filter press to perform solid-liquid separation, to prepare a filtrate;

[0070] (4) placing the filtrate prepared in step (3) in an inactivation device to perform inactivation treatment, to prepare a water-soluble fertilizer.

[0071] Example 4

[0072] A water-soluble fertilizer is prepared from raw materials including the following components by weight: soybean meal powder 65 parts, seaweed extract 10 parts, organic nitrogen phosphorus potassium source 5 parts, mannitol 4 parts, glucose 4 parts, seaweed polysaccharide 4 parts, polyglutamic acid 4 parts, salicylic acid 0.06 parts, fermentation bacteria 0.3 parts, composite enzyme 0.2 parts, modifier 0.8 parts, and water 140 parts.

[0073] The organic nitrogen phosphorus potassium source is brown sugar.

[0074] The composite enzyme is composed of cellulase, xylitolase, and papain at a mass ratio of 3:4:0.7.

[0075] The modifier is prepared from polyacrylamide, hydroxyapatite, and humic acid at a mass ratio of 1:4:3.

[0076] The fermentation bacteria are composed of yeast, lactic acid bacteria, and bacillus licheniformis at a mass ratio of 8:0.6:2.

[0077] A method for using the composite enzyme in the manufacture of a water-soluble fertilizer, comprising the following steps:

[0078] (1) preparing soybean meal powder from soybeans, for standby use;

[0079] (2) mixing and stirring soybean meal powder, seaweed extract, organic nitrogen phosphorus potassium source, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, composite enzyme, modifier, and water uniformly, and then performing fermentation enzymolysis at a temperature of 28℃ for 6 days to prepare a fermentation enzymolysis solution;

[0080] (3) using a filter tank to filter the fermentation enzymolysis solution prepared in step (2) and then placing it in a filter press to perform solid-liquid separation, to prepare a filtrate;

[0081] (4) placing the filtrate prepared in step (3) in an inactivation device to perform inactivation treatment, to prepare a water-soluble fertilizer.

[0082] Example 5

[0083] A water-soluble fertilizer is made from raw materials including soybean meal powder 70 parts, seaweed extract 12 parts, organic nitrogen phosphorus potassium source 7 parts, mannitol 8 parts, glucose 2 parts, seaweed polysaccharide 8 parts, polyglutamic acid 4 parts, salicylic acid 0.05 parts, fermentation bacteria 0.3 parts, composite enzyme 0.2 parts, modifier 0.5 parts, and water 120 parts.

[0084] The organic nitrogen phosphorus potassium source is brown sugar.

[0085] The composite enzyme is composed of cellulase, xylitolase, and papain in a mass ratio of 2:5:0.6.

[0086] The modifier is made from polyacrylamide, hydroxyapatite, and humic acid in a mass ratio of 0.5:2:3.

[0087] The fermentation bacteria are composed of yeast, lactic acid bacteria, and bacillus licheniformis in a mass ratio of 4:0.4:1.

[0088] The use of the composite enzyme in the manufacture of water-soluble fertilizer includes the following steps:

[0089] (1) Soybeans are processed into soybean meal powder for use;

[0090] (2) The soybean meal powder, seaweed extract, organic nitrogen phosphorus potassium source, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, composite enzyme, modifier, and water are mixed and stirred uniformly, then subjected to fermentation and enzymatic hydrolysis at a temperature of 30°C for 6 days to produce a fermentation and enzymatic hydrolysis liquid;

[0091] (3) The fermentation and enzymatic hydrolysis liquid produced in step (2) is filtered using a filter tank and then subjected to solid-liquid separation in a filter press to produce a filtrate;

[0092] (4) The filtrate produced in step (3) is subjected to inactivation treatment in an inactivation device to produce a water-soluble fertilizer.

[0093] Comparative Example 1

[0094] It is basically the same as Example 3, except that the modifier is missing from the raw materials used to prepare the water-soluble fertilizer, and the difference is made up with water.

[0095] Comparative Example 2

[0096] It is basically the same as Example 3, except that polyacrylamide is missing from the raw materials used to prepare the water-soluble fertilizer, and the difference is made up with water.

[0097] Comparative Example 3

[0098] It is basically the same as Example 3, except that hydroxyapatite is missing from the raw materials used to prepare the water-soluble fertilizer, and the difference is made up with water.

[0099] Comparative Example 4

[0100] The same as example 3, except that the raw material for preparing the water-soluble fertilizer lacks humic acid, and the difference is made up with water.

[0101] Comparative example 5

[0102] The same as example 3, except that the raw material for preparing the water-soluble fertilizer lacks the composite enzyme, and the difference is made up with water.

[0103] Comparative example 6

[0104] The same as example 3, except that the raw material for preparing the water-soluble fertilizer lacks the xylitol enzyme, and the difference is made up with water.

[0105] Comparative example 7

[0106] The same as example 3, except that the raw material for preparing the water-soluble fertilizer lacks the cellulase, and the difference is made up with water.

[0107] Comparative example 8

[0108] The same as example 3, except that the raw material for preparing the water-soluble fertilizer lacks the papain, and the difference is made up with water.

[0109] (I) Application of the water-soluble fertilizer

[0110] An experimental base was selected in Suncun, Santang Town, Xingning District, Nanning City, 13 plots of land with basically consistent conditions were selected, each plot was 1 mu, and the same variety of tomatoes were planted. From the tomato seedling stage, the water-soluble fertilizers prepared by examples 1-5 and comparative examples 1-8 were sprayed, the use method was: 200 times dilution with water before use, the dosage per mu was 200 mL, and the fertilizer was applied once every 7 days, a total of three times, and the other planting process conditions were basically consistent. After the tomatoes were matured, the yield per mu was measured, and the results are shown in the following table.

[0111] Group Yield per mu (kg) Example 1 4790 Example 2 4843 Example 3 4864 Example 4 4793 Example 5 4826 Comparative Example 1 3675 Comparative Example 2 4712 Comparative Example 3 4567 Comparative Example 4 4421 Comparative Example 5 4071 Comparative Example 6 4608 Comparative Example 7 4552 Comparative Example 8 4734

[0112] As shown in the above table, using the water-soluble fertilizer of the present application, the yield per mu of tomatoes in examples 1-5 reached 4790 kg or more, and the yield per mu of tomatoes in example 3 reached 4864 kg, greatly improving the yield of crops and economic benefits.

[0113] As can be seen from comparative examples 1-4, the polyacrylamide, hydroxyapatite and humic acid in the improver of the present application produce a synergistic reaction, which can act together to improve the yield per mu of tomatoes and further improve the economic benefits.

[0114] As can be seen from comparative examples 5-8, the xylitol enzyme, cellulase and papain of the present application also produce a synergistic reaction, which further improves the yield per mu of tomatoes and promotes economic improvement.

Claims

1. A method for using a complex enzyme in the manufacture of a water-soluble fertilizer, characterized in that: The following steps are involved: (1) Prepare soybean meal powder from soybeans and set aside; (2) mixing soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium source, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, fermentation bacteria, complex enzyme, improver and water, and then performing fermentation and enzymolysis to obtain fermentation enzymolysis liquid; (3) filtering the fermentation enzymatic hydrolysate obtained in step (2) using a filter tank and placing the filtered liquid into a filter press for solid-liquid separation to obtain a filtrate; (4) placing the filtrate obtained in step (3) in an inactivation device for inactivation treatment to obtain a water-soluble fertilizer; The complex enzyme is composed of cellulase, xylitolase and papain in a mass ratio of 2-4:1-5:0.5-0.7; The step (2) comprises mixing and stirring the following raw materials in parts by weight: 60-70 parts of soybean meal powder, 10-15 parts of seaweed extract, 5-7 parts of organic nitrogen, phosphorus and potassium source, 4-8 parts of mannitol, 2-5 parts of glucose, 4-8 parts of seaweed polysaccharide, 2-4 parts of polyglutamic acid, 0.04-0.08 parts of salicylic acid, 0.3-0.5 parts of fermentation bacteria, 0.1-0.2 parts of complex enzyme, 0.2-0.8 parts of improver, and 120-200 parts of water; The improver is made of polyacrylamide, hydroxyapatite and humic acid in a mass ratio of 0.5-1:2-5:2-3; The fermentation bacteria are composed of yeast, lactic acid bacteria and Bacillus licheniformis in a mass ratio of 4-8:0.3-0.6:1-2.

2. The method for using the complex enzyme in the manufacture of water-soluble fertilizer according to claim 1, characterized in that: The complex enzyme consists of cellulase, xylitolase and papain in a mass ratio of 3:4:0.

6.

3. The method for using the complex enzyme in the manufacture of water-soluble fertilizer according to claim 1, characterized in that: The organic nitrogen, phosphorus and potassium source is brown sugar.

4. The method for using the complex enzyme in the manufacture of water-soluble fertilizer according to claim 1, characterized in that: The improver is prepared from polyacrylamide, hydroxyapatite and humic acid in a mass ratio of 0.8:4:2.

7.

5. The method for using the complex enzyme in manufacturing water-soluble fertilizer according to claim 1, characterized in that: The fermentation bacteria are composed of yeast, lactic acid bacteria and Bacillus licheniformis in a mass ratio of 6:0.5:1.

3.

6. The method for using the complex enzyme in manufacturing water-soluble fertilizer according to claim 1, characterized in that: The conditions for fermentation and enzymolysis in step (2) are as follows: fermentation and enzymolysis are carried out at a temperature of 25-32° C. for 5-8 days.