A DNA / RNA synthesizer

Abstract

A DNA / RNA synthesis device belongs to the field of DNA recombination technology, including the body, the cavity is arranged in the body, the cavity is provided with the partition plate group, the cavity is divided into ventilation chamber, synthesis chamber and reagent chamber according to its use;The synthesis chamber is built-in mounting plate, and the synthesis chamber is divided into synthesis cavity and electronic cavity, the synthesis cavity is equipped with CPG synthesis module for realizing DNA / RNA synthesis, and CPG liquid adding module for reagent liquid adding and quantitative liquid adding module connected with CPG liquid adding module, the quantitative liquid adding module is installed on the mounting plate, and the quantitative liquid adding module is accommodated in the electronic cavity;Through modularization, it is convenient to expand and maintain later, without considering the economic loss caused by replacing the whole device due to the damage of a component, in addition, through CPG synthesis module, CPG liquid adding module and quantitative liquid adding module connected with CPG liquid adding module, high-throughput synthesis efficiency can be realized, and the purpose of quantitative liquid adding can be realized.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of DNA recombination technology, and particularly relates to a DNA / RNA synthesis device. BACKGROUND

[0002] Synthetic biology is the third biological technology revolution after the discovery of DNA double helix leading to molecular biology and the implementation of human genome project leading to genomics. DNA synthesis technology is one of the core technologies of synthetic biology, which is widely used in protein modification and life science and other fields, such as nucleic acid drugs, enzyme engineering, gene therapy, etc. DNA synthesizer is an important instrument and equipment for biological research and development. The principle of DNA synthesis mainly includes chemical method and biological method. Among them, the chemical method (especially the solid-phase phosphoramidite triester synthesis method) is the most mature and widely used, which can synthesize various DNA / RNA oligonucleotide fragments (long-chain and super-long-chain primer fragment synthesis, fluorescently labeled and specially modified primer synthesis, milligram and gram-scale large-scale primer synthesis, siRNA and modified RNA synthesis, etc.), and can perform specific chemical modification and modification, introduce molecular probes and special modified bases, etc. With the development of more and more biological industries, the demand for DNA synthesis is also increasing. For example, in the development of COVID-19 vaccine, after the design of the vaccine sequence, samples need to be quickly obtained for experimental verification, and multiple vaccine sequence samples need to be designed for parallel experiments. The synthesis sample throughput of the vaccine reaches K level, and the yield is in fmol level. However, the synthesis of vaccine samples usually needs to wait for several days or weeks, which delays the subsequent experimental process. There is a lack of high-throughput, high-quality and low-cost DNA synthesis instrument in the market, especially medium and high-throughput DNA synthesis instruments, which cannot meet the demand of rapid DNA synthesis and need to be solved urgently. SUMMARY

[0003] The present application aims to provide a DNA / RNA synthesis device to solve the problems in the background art.

[0004] To achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0005] A DNA / RNA synthesis device, comprising a body, wherein the body is internally hollow to form a cavity, the cavity is internally provided with a partition plate group, and the cavity is divided into a ventilation chamber, a synthesis chamber and a reagent chamber according to their purposes; the synthesis chamber is internally provided with a first mounting plate, and the synthesis chamber is divided into a synthesis cavity and an electronic cavity; the synthesis cavity is internally provided with a CPG synthesis module for realizing DNA / RNA synthesis and a CPG liquid adding module for adding reagents; the electronic cavity is internally provided with a quantitative liquid adding module connected with the CPG liquid adding module in a pipeline manner; and the quantitative liquid adding module is mounted on the first mounting plate.

[0006] Compared with the prior art, the technical scheme has the following effects:

[0007] Firstly, the modularization facilitates the later expansion and maintenance without considering the economic loss caused by replacing the entire device due to damage of a component, in addition, the CPG synthesis module, the CPG liquid adding module and the quantitative liquid adding module connected to the CPG liquid adding module realize high-throughput synthesis efficiency while achieving the purpose of quantitative liquid adding.

[0008] As preferred, the device further comprises a gas circulation system for constructing internal circulation of the gas in the body.

[0009] As preferred, the gas circulation system comprises an adsorption box built in the ventilation chamber, the adsorption box is connected with an exhaust duct, and the adsorption box is located above the synthesis cavity.

[0010] As preferred, it further comprises a first axial flow fan and a drying tube built in the electronic cavity, one end of the drying tube extends to the outside of the body and is connected with the outside, the other end penetrates through the first mounting plate and is accommodated in the synthesis cavity, and the first axial flow fan is connected with the electronic cavity through a duct.

[0011] As preferred, it further comprises a second axial flow fan, the second axial flow fan is connected with a connecting pipe, and the connecting pipe is connected with the reagent chamber through a duct.

[0012] As preferred, the CPG synthesis module comprises a liquid discharge plate, the upper end surface of the liquid discharge plate is provided with a CPG module through bolts; the upper end surface of the liquid discharge plate is recessed inward to form a guide cavity for guiding the synthesis reagent, the guide cavity is connected with a liquid discharge hole, the liquid outlet end of the CPG module extends to the inside of the guide cavity, and the liquid discharge hole is connected with a negative pressure assembly through a duct.

[0013] As preferred, the CPG module comprises a CPG carrier plate, the lower end surface of the CPG carrier plate is provided with a CPG distribution plate, and the liquid outlet end of the CPG carrier plate is sintered with a CPG column, the liquid outlet end of the CPG column abuts against the upper surface of the CPG distribution plate, and the upper end surface of the CPG distribution plate is hot-pressed with a filter membrane.

[0014] As preferred, the CPG liquid adding module comprises a mounting plate, the upper end surface of the mounting plate is bolted with an assembly plate, a plurality of liquid adding needle tubes are arranged on the assembly plate in an array, and the lower ends of the liquid adding needle tubes extend to the lower end surface of the mounting plate; the lower end surface of the assembly plate is arranged with a plurality of empty grooves in an array, the empty grooves are built with elastic pressing plates for locking the liquid adding needle tubes, and the upper end wall of the empty grooves is provided with an unlocking hole.

[0015] As preferred, the quantitative liquid adding module comprises a valve seat, a first valve is arranged on the valve seat, the first valve is connected with a liquid inlet connector in pipeline, and the first valve is connected with a plurality of second valves in pipeline, the output end of the second valve is connected with a third valve in pipeline, and the output end of the third valve is connected with a liquid outlet connector in pipeline; wherein, when liquid is added, the first valve and the second valve are in an open state, and the third valve is in a closed state; when liquid is discharged, the first valve is closed, and the second valve and the corresponding third valve are opened. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is the first schematic diagram of the overall structure of the present application;

[0017] Figure 2 is the second schematic diagram of the overall structure of the present application;

[0018] Figure 3 is the schematic diagram of the local structure in the present application;

[0019] Figure 4 is the first schematic diagram of the overall structure of the quantitative liquid adding module in the present application;

[0020] Figure 5 is the second schematic diagram of the overall structure of the quantitative liquid adding module in the present application;

[0021] Figure 6 is the schematic diagram of the left view structure of the quantitative liquid adding module in the present application;

[0022] Figure 7 is the schematic diagram of the local structure at "B-B" in the present application; Figure 6

[0023] is the schematic diagram of the local structure at "C-C" in the present application; Figure 8 Figure 6

[0024] Figure 9 is the schematic diagram of the overall structure of the CPG synthesis module in the present application;

[0025] Figure 10 is the schematic diagram of the overall cross-sectional structure of the CPG synthesis module in the present application;

[0026] Figure 11 is the first schematic diagram of the exploded structure of the CPG synthesis module in the present application;

[0027] Figure 12 is the second schematic diagram of the exploded structure of the CPG synthesis module in the present application;

[0028] Figure 13 is the enlarged schematic diagram of the local structure at "A" in the present application; Figure 10

[0029] ​​​Figure 14 is the first schematic diagram of the partial explosion structure of the CPG synthesis module in the present application;

[0030] Figure 15 is the second schematic diagram of the partial explosion structure of the CPG synthesis module in the present application;

[0031] Figure 16 is the schematic diagram of the partial assembly sectional structure of the CPG synthesis module in the present application;

[0032] Figure 17 is the schematic diagram of the overall structure of the CPG liquid adding module in the present application;

[0033] Figure 18 is the first schematic diagram of the overall structure explosion of the CPG liquid adding module in the present application;

[0034] Figure 19 is the second schematic diagram of the overall structure explosion of the CPG liquid adding module in the present application;

[0035] Figure 20 is the schematic diagram of the partial structure explosion amplification of the CPG liquid adding module in the present application;

[0036] Figure 21 is the schematic diagram of the overall structure of the elastic pressing plate in the CPG liquid adding module in the present application. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments of the present application.

[0038] Embodiment:

[0039] The meaning of CPG in the present embodiment is controlled pore glass, controllable microporous glass beads.

[0040] As shown in the DNA / RNA synthesis device shown in Figures 1-2 , comprising a body 1, the body 1 is surrounded by a plurality of side plates to form a cavity box, and the cavity inside is sequentially provided with a first partition plate 8 and a second partition plate 3 from top to bottom, which divides the cavity into three areas, which are ventilation chamber, synthesis chamber and reagent chamber according to the functions of the three areas;

[0041] Further, the synthesis chamber is provided with a first mounting plate, which divides the synthesis chamber into a synthesis cavity and an electronic cavity, the synthesis cavity is provided with related working components required for DNA recombination, the working components include a moving assembly 10 for realizing XY axial movement, a CPG liquid adding module 13, a CPG synthesis module 12 and a quantitative liquid adding module 14, and the specific structure is further described below, and the electronic cavity is used for mounting the quantitative liquid adding module 14.

[0042] It is worth noting that the body 1 is provided with a gas circulation system inside, which is used for guaranteeing the temperature and humidity requirements of the DNA synthesis environment, and effectively reducing the emission of toxic gas to meet the environmental protection requirements.

[0043] The gas circulation system includes an adsorption box 9 arranged in the ventilation cavity, and the adsorption box 9 is connected with an exhaust duct 2, and the exhaust duct 2 is arranged at the top of the body 1, in this embodiment, the adsorption box 9 includes an adsorption box body, the adsorption box body is hollow inside, and a plurality of air holes are formed in the surface of the adsorption box body, so as to realize the communication between the ventilation cavity and the outside, because the types of reagents used in the synthesis process are different, some will volatilize into the gas inside the body 1, therefore, direct emission is avoided, and the adsorption box body is filled with activated carbon for adsorbing toxic substances.

[0044] Secondly, the adsorption box 9 and the exhaust duct 2 are provided with an air extraction device (not shown in the figure), more specifically, the air extraction end of the air extraction device is connected with the adsorption box 9, and the air outlet end of the air extraction device is connected with the exhaust duct 2, at the same time, the adsorption box 9 is located above the synthesis cavity, when the air extraction device is started, the gas in the synthesis cavity will be adsorbed by the adsorption box 9 and discharged to the outside.

[0045] In addition, the electronic cavity is provided with a drying tube 4, one end (air inlet end) of which is communicated with the outside through the machine body 1, and the other end (air inlet end) is communicated with the synthetic cavity through the first mounting plate, so as to ensure the drying of the gas entering the machine body 1 and provide a stable synthetic environment. The drying tube 4 is provided with silica gel moisture absorbing particles. In actual application, part of the gas in the synthetic cavity will flow into the electronic cavity. Therefore, the upper portion of the electronic cavity is provided with a first axial flow fan 6, the suction end of which is connected to the electronic cavity, and the first axial flow fan 6 is accommodated in the ventilation chamber, so that when the first axial flow fan 6 is turned on, a negative pressure chamber is formed in the electronic cavity, and the gas is discharged into the ventilation chamber. At the same time, the ventilation chamber is also provided with a second axial flow fan 7, which is connected with a connecting pipe 5, and the connecting pipe 5 is connected to the reagent cavity. The first axial flow fan 6, the second axial flow fan 7, the adsorption box 9, the drying tube 4 and the exhaust pipe 2 constitute a complete internal gas circulation system of the machine body 1.

[0046] In addition, in the embodiment, modular concept is adopted to realize liquid adding, synthesis and waste liquid collection, etc., so as to solve the problem of inconvenient expansion of the existing equipment.

[0047] That is, in the embodiment, a moving assembly 10 for moving in XY directions is realized. This kind of setting in the prior art is described more, and will not be repeated here. However, it should be noted that the moving end of the moving assembly 10 for driving the synthesis module 12 to move in Y direction is provided with a tray 11 for mounting the synthesis module 12. The tray 11 is convenient for expanding the synthesis module 12 to provide synthesis flux, that is, the originally single synthesis module 12 is arrayed and copied.

[0048] The specific structure of the CPG synthesis module 12 is referred to Figures 9-16It can be known that the CPG synthesis module 12 comprises a CPG carrier plate 12020, a CPG distribution plate 121 and a drainage plate 122 arranged in sequence from top to bottom, and the CPG carrier plate 12020, the CPG distribution plate 121 and the drainage plate 122 are locked by bolts 126. Specifically, the CPG carrier plate 12020 comprises a carrier plate body, the upper end of the carrier plate body is recessed inward to form a recessed cavity 130, the lower end of the recessed cavity 130 is arrayed with a plurality of synthesis holes, and the edge of the upper end of the carrier plate body is provided with a plurality of threaded holes 128, the threaded holes 128 are internally provided with the bolts 126, the lower end of the bolt 126 is connected to the distribution plate 121, that is, the position of the distribution plate 121 for connecting the bolt 126 is provided with a connecting hole 12100. It should be noted that the threaded holes 128 and the connecting hole 12100 are not as literally meant, that is, the threaded holes 128 must be provided on the carrier plate body, and the connecting hole 12100 must be provided on the distribution plate 121, which is only for better distinction and exact expression.

[0049] Further, the edge of the upper end of the carrier plate body is provided with a waist-shaped groove 129, and the waist-shaped groove 129 and the threaded holes 128 are arranged alternately. The waist-shaped groove 129 has the following advantages: 1. The CPG carrier plate 12020 in the embodiment is prepared by injection molding process, which meets the technical requirements of uniform wall thickness of the injection molding process; 2. The groove wall of the waist-shaped groove 129 constitutes a rib plate of the carrier plate, thereby strengthening the structural strength of the carrier plate itself.

[0050] In addition, the CPG carrier plate 12020 is made of high-density PP material and is prepared by injection molding process, and a plurality of liquid inlet channels are arrayed on the CPG carrier plate 12020. The liquid inlet channels comprise, in sequence from top to bottom, a liquid inlet section 1200, a transition section 1201 and a CPG carrier section 1202, which are connected in sequence, that is, the lower end of the liquid inlet section 1200 is connected to the upper end 1201 of the transition section 1201, and the lower end of the transition section 1201 is connected to the upper end of the CPG carrier section 1202. In the embodiment, the liquid inlet section 1200, the transition section 1201 and the CPG carrier section 1202 are integrally formed. In an ideal state, the inner surfaces formed by the three sections should be arc surfaces, but based on the technical difficulty in the real scene, the following scheme is adopted: the inner diameter of the liquid inlet section 1200 is greater than the inner diameter of the CPG carrier section 1202, and the inner surface of the transition section 1201 is a bevel. The transition section 1201 has a bevel structure, which is convenient for injection molding and has stable dimensions. At the same time, the transition section 1201 connects the liquid inlet section 1200 and the CPG carrier section 1202 in communication to achieve a buffering effect. In addition, the smooth surface avoids dead angles and is conducive to the flow of reagents and the cleaning of reagents.

[0051] Secondly, the CPG carrier section 1202 is provided with a CPG column a, referring to Figure 3 and Figure 4 The CPG column a shown in the figure only represents the position relationship of being arranged in the synthesis hole 11 and the corresponding distribution plate 2, and the specific relationship will be described again below, so it is not expressed emphatically here, but it should be noted that the CPG column a used in this embodiment is not prepared in advance, but prepared by sintering process, and the specific preparation process is as follows:

[0052] (1) Preparation of raw materials: In this embodiment, 2000 pore CPG powder and ultra-high molecular weight polyethylene are selected as raw materials, wherein the CPG powder is non-silanized CPG powder (referred to as blank CPG powder);

[0053] (2) Preparation content: A part of the blank CPG powder in the CPG powder is silanized to obtain silanized CPG powder, then the blank CPG powder and the silanized CPG powder are premixed according to the selected CPG molar concentration, and then the premixed blank CPG powder and the silanized CPG powder are premixed with the ultra-high molecular weight polyethylene to obtain a mixed powder. The mixed powder is sieved into the CPG carrier section 1202 by a vibrating screen, and the CPG column a is prepared by a sintering process, so as to meet the requirement of the integrated structure of the CPG column a and the CPG carrier plate 1.

[0054] Due to the small pore size, if the CPG column a is prepared in advance and filled into the CPG carrier section 1202, the operation requirement will be more strict, and the installation precision of the CPG column a in each synthesis hole will be different due to external factors during installation, which will cause the difference of CPG gas resistance and finally affect the synthesis efficiency and quality of DNA chain.

[0055] Among them, the CPG carrier plate 12020 is prepared by injection molding process, and at the same time, in order to ensure the sintering of the subsequent CPG column a, the inner surface of the liquid inlet channel in this embodiment needs to achieve the effect of mirror high light finish, and then spray Teflon coating to reduce the problem of synthetic reagent infiltrating the hydrophobic surface and reducing the surface residue of synthetic reagent, solve the subsequent cleaning problem, such as: low cleaning efficiency or large amount of cleaning agent.

[0056] In this embodiment, in order to avoid residue of liquid discharge and improve the effect of liquid discharge, the upper end surface of the liquid discharge plate 122 is concave to form a guide cavity 1220, the guide cavity 1220 is provided with a liquid discharge hole 1221 in communication, and the liquid discharge plate 122 is provided with a tracheal connector 123, the tracheal connector 123 is connected to the liquid discharge hole 1221 in a pipeline manner, combined with Figure 10 and Figure 11It can be known that the liquid inlet end of the liquid outlet hole 1221 is arranged at the middle position of the bottom wall of the guide cavity 1220, and the inner wall of the guide cavity 1220 in this embodiment is a slope, the bottom of the slope points to the liquid outlet hole 1221, thereby achieving the purpose of drainage.

[0057] Further, the CPG distribution plate 121 is provided with a plurality of distribution channels 1212, and the liquid outlet ends of the plurality of distribution channels 1212 extend downward, and in the assembled state, the extension parts of the liquid outlet ends of the plurality of distribution channels 1212 are accommodated in the guide cavity 1220, so that the splashing problem during distribution can be effectively avoided.

[0058] At the same time, in order to further improve the sealing effect of the device, the lower end surface of the CPG distribution plate 121 is provided with a sealing ring 125, and the sealing ring 125 is embedded in the upper end surface of the liquid outlet plate 122 during assembly.

[0059] In addition, the device also includes a filter membrane 124 arranged between the CPG carrier plate 120 and the CPG distribution plate 121, and the lower end surface of the filter membrane 124 is pressed against the upper end surface of the CPG distribution plate 121, and in this state, the lower end of the CPG column a in the device abuts against the upper surface of the filter membrane 124.

[0060] In addition, since the synthetic module 12 contains a tracheal joint 123, after expansion, it is equivalent to containing a plurality of tracheal joints 123, and in this embodiment, the gas path of each synthetic module is controlled separately, that is, each tracheal joint 123 is connected with a valve, and the tracheal joint 123 is connected to the vacuum tank, which can be the same or each tracheal joint 123 is connected with one.

[0061] Reference Figures 4-8 It can be known that the quantitative microliter liquid adding module 14 in the device includes a valve seat 140, the front side of the right end surface of the valve seat 140 is provided with a liquid inlet joint 141, the liquid inlet joint 141 is connected with a first valve 143, the first valve 143 is arranged on the front side of the upper end surface of the valve seat 140, and a plurality of second valves 144 are arranged on the rear side of the first valve 143, and according to the front projection direction, the plurality of second valves 144 and the first valve 143 coincide, and a plurality of third valves 145 are arranged on the left end surface of the valve seat 140, in this embodiment, the valve is fixed on the valve seat 140 by bolts, which is convenient for replacement.

[0062] In addition, in this embodiment, the first valve 143, the second valve 144 and the third valve 145 are selected as electromagnetic valves, which can better realize the quantitative liquid adding effect.

[0063] Combining Figures 7-8 It can be known that the direction of the black arrow in the drawing is the flow direction of the reagent in the pipeline, and the valve seat 140 is internally provided with a pipeline system to realize multiple working modes of the liquid adding module 14. The pipeline system specifically includes a main pipeline 1400 which penetrates through the front and rear ends of the valve seat 140, and the main pipeline 1400 is provided with a plug 146 at the positions penetrating through the front and rear ends of the valve seat, so as to ensure the sealing property and avoid liquid leakage.

[0064] Secondly, the liquid inlet connector 141 is connected with a first pipeline 1402, the liquid outlet end of the first pipeline 1402 is connected with a first valve 143, the output end of the first valve 143 is connected with a second pipeline 1401, and the second pipeline 1401 is connected with the main pipeline 1400. It is worth noting that in the embodiment, the input ends of the second valves 144 are all connected with the main pipeline 1400.

[0065] The output end of the second valve 144 is connected with a third pipeline 1402, the third pipeline 1402 is connected with the input end of a third valve 145, the output end of the third valve 145 is connected with a fourth pipeline 1403, and the fourth pipeline 1403 is connected with the liquid outlet connector 142.

[0066] In the actual application scene, the first valve 143 and the second valve 144 are opened, and the third valve 145 is closed. At this time, the reagent is stored in the liquid adding module through the liquid inlet connector 141 and the corresponding pipelines in the pipeline system, and the purpose of quantitative liquid adding is realized through the internal volume of the valve itself and the incompressibility of the liquid.

[0067] It is worth mentioning that when the first valve 143, the second valve 144 and the third valve 145 are all opened, the internal cleaning work of the liquid adding module can be carried out, or the purpose of continuous liquid adding can be realized, but the precision of liquid adding is slightly insufficient.

[0068] Furthermore, in combination Figures 17-20 The device further includes a mounting plate 130, the upper end surface of the mounting plate 130 is bolted with an assembly plate 131, the assembly plate 131 is arrayed with liquid adding needle tubes 132, and the lower ends of the liquid adding needle tubes 132 penetrate through the lower end surface of the mounting plate 130. The mounting plate 130 is used for mounting on a moving assembly to realize the position adjustment of the liquid adding nozzle module.

[0069] It is worth mentioning that by changing the liquid adding needle tube 132 from the traditional integrated structure to the existing segmented structure, the problem of difficult processing of small size whole is solved, as follows: the liquid adding needle tube 132 comprises a first tube body 1320, a second tube body 1322 is inserted at the lower end of the first tube body 1320, and a clamping member 1321 is sleeved on the outside of the lower end of the first tube body 1320. In this embodiment, the clamping member 1321 is made of metal, and on one hand, the second tube body 1322 is fastened to the lower end of the first tube body 1320 through the clamping member 1321 to achieve the sealing effect; on the other hand, it realizes the purpose of limiting together with the mounting plate 130.

[0070] The specific structure of the mounting plate 130 and the assembly plate 131 is described as follows: the mounting plate 130 comprises a mounting plate body, the mounting plate body is provided with a first mounting hole 1300 for mounting the mounting plate body on the moving assembly, and two rows of second mounting holes 1302 are arranged on the mounting plate body in front of and behind each other, and a penetrating hole 1301 is arranged between the two rows of second mounting holes 1302, and the clamping member 1321 is accommodated in the penetrating hole 1301.

[0071] The assembly plate 131 comprises an assembly plate body, two rows of third mounting holes 1310 are arranged on the assembly plate body in front of and behind each other, and the two rows of third mounting holes 1310 and the two rows of second mounting holes 1302 on the mounting plate body correspond one by one, that is, from top to bottom, the third mounting hole 1310 and the second mounting hole 1302 coincide, and the assembly hole 1314 is arranged at the interval of the two rows of third mounting holes 1310, and the tube part of the liquid adding needle tube 132 is accommodated in the assembly hole 1314. In this embodiment, one side (i.e. the so-called right side) of the assembly hole 1314 is provided with an unlocking hole 1311, and the lower end surface of the assembly plate body is arranged with a hollow groove 1312, and the hollow groove 1312 is arranged with a partition plate 1313 inside, which divides the inside of the hollow groove 1312 into several areas, and each area of the several areas corresponds to a lock hole group (i.e. the combination of one assembly hole 1314 and one unlocking hole 1311), and the several areas are communicated with each other. More specifically, the width of the partition plate 1313 is smaller than the width of the hollow groove 1312, and the purpose of this setting is as follows: first, it is relatively easy to process, and it is more economical in manufacturing cost; second, it provides convenience for subsequent disassembly and replacement of single liquid adding needle tube 132.

[0072] Specifically how to provide convenience for disassembly and replacement of the single liquid adding needle tube 132, the related structure in the specific embodiment is combined to be described, in the embodiment, the elastic pressing plate 133 is built in the empty slot 1312, and the elastic pressing plate 133 realizes the purpose of fixing the part of the pipe body of the liquid adding needle tube 132 contained in the empty slot 1312;

[0073] In the embodiment, the elastic pressing plate 133 is built in the empty slot 1312, and the elastic pressing plate 133 realizes the purpose of fixing the part of the pipe body of the liquid adding needle tube 132 contained in the empty slot 1312; Figure 20 It can be known that the elastic pressing plate 133 includes a plate body 1330, the upper end of the plate body 1330 is provided with a mounting end, the mounting end is arrayed with a plurality of connecting pieces 1331, the plurality of connecting pieces 1331 are all fixedly connected with connecting rods 1332, the lower end of the connecting rod 1332 is transversely provided with a pressing block 1333, that is, the pressing block 1333 is contained in each region separated by the plurality of partition plates 1313, the elastic pressing plate 133 is more stably installed in the empty slot 1312, and meanwhile, the abutting end surface of the pressing block 1333 is provided with an arc surface 13330, the arc surface 13330 can better contact the outer wall of the pipe body of the liquid adding needle tube 132, so that the installation of the liquid adding needle tube 132 is more stable, of course, in order to further enhance the practicability of the device, a flexible layer can be arranged on the arc surface 13330, so as to avoid damage to the pipe body of the liquid adding needle tube 132 caused by the pressure generated by abutting, and it should be noted that the plate body 1330, the connecting piece 1331, the connecting rod 1332 and the pressing block 1333 are all made of metal and are integrated structures, in a specific application scenario, when a single liquid adding needle tube 132 is disassembled, a tool is used to press the connecting rod 1332 through the unlocking hole 1311, so that the arc surface 13330 on the pressing block 1333 is separated from the outer wall of the liquid adding needle tube 132, at this time, the user pulls the single liquid adding needle tube 132 upward, so that the single liquid adding needle tube 132 is replaced.

[0074] In the description of the application, it should be understood that the terms "center", "transverse", "upper", "lower", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the application. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more of the features. In the description of the application, unless otherwise stated, the meaning of "several" is two or more. In addition, the term "includes" and any variation thereof is intended to cover non-exclusive inclusion.

[0075] The application is illustrated by the examples, and the device can be modified and improved in several ways without departing from the principle thereof. It should be pointed out that any technical solution obtained by using equivalent substitution or equivalent transformation, etc. falls within the protection scope of the application.

Claims

1. A DNA / RNA synthesis device, comprising a body (1), a cavity is hollowed in the inside of the body (1), a group of partition plates is arranged in the inside of the cavity, and the cavity is divided into a ventilation chamber, a synthesis chamber and a reagent chamber according to its use; characterized in that: The synthesis chamber is internally provided with a first mounting plate, which separates the synthesis chamber into a synthesis cavity and an electronic cavity, the synthesis cavity is internally provided with a CPG synthesis module (12) for realizing DNA / RNA synthesis and a CPG liquid adding module (13) for adding reagents, and the electronic cavity is internally provided with a quantitative liquid adding module (14) which is pipeline-connected with the CPG liquid adding module (13), and the quantitative liquid adding module (14) is mounted on the first mounting plate. The CPG synthesis module (12) comprises a liquid discharging plate (122), the upper end face of the liquid discharging plate (122) is provided with a CPG module through a bolt (126); the upper end face of the liquid discharging plate (122) is inwardly recessed to form a guide cavity (1220) for guiding the flow of synthesis reagents, the guide cavity (1220) is communicatively provided with a liquid discharging hole (1221), and the liquid outlet end of the CPG module extends into the guide cavity (1220), and the liquid discharging hole (1221) is pipeline-connected with a negative pressure assembly. The CPG module comprises a CPG carrier plate (120), the lower end face of the CPG carrier plate (120) is provided with a CPG distribution plate (121), and the liquid outlet end of the CPG carrier plate (120) is sintered with a CPG column (a), the liquid outlet end of the CPG column (a) abuts against the upper surface of the CPG distribution plate (121), and the upper end face of the CPG distribution plate (121) is hot-pressed with a filter membrane (124). The CPG liquid adding module (13) comprises a mounting plate (130), the upper end face of the mounting plate (130) is bolt-connected with an assembly plate (131), a plurality of liquid adding needle tubes (132) are arrayed on the assembly plate (131), and the lower ends of the liquid adding needle tubes (132) extend to the lower end face of the mounting plate (130).

2. A DNA / RNA synthesizer as defined in claim 1, wherein: The device further comprises a gas circulation system for establishing internal circulation of construction gas in the body (1).

3. A DNA / RNA synthesizer as defined in claim 2, wherein: The gas circulation system comprises an adsorption box (9) internally provided in the ventilation chamber, the adsorption box (9) is pipeline-connected with an exhaust duct (2), and the adsorption box (9) is located above the synthesis cavity.

4. A DNA / RNA synthesizer as defined in claim 3, wherein: Further comprising a first axial flow fan (6) and a drying tube (4) internally provided in the electronic cavity, one end of the drying tube (4) extends to the outside of the body (1) and is connected with the outside, the other end of the drying tube (4) penetrates through the first mounting plate and is accommodated in the synthesis cavity, and the first axial flow fan (6) is pipeline-connected with the electronic cavity.

5. A DNA / RNA synthesizer as defined in claim 4, wherein: Further comprising a second axial flow fan (7), the second axial flow fan (7) is pipeline-connected with a connecting pipe (5), and the connecting pipe (5) is pipeline-connected with the reagent chamber.

6. The DNA / RNA synthesizer of claim 1, wherein: The lower end face of the assembly plate (131) is arrayed with a plurality of empty grooves (1312), the empty grooves (1312) are internally provided with elastic pressing plates (133) for locking the liquid adding needle tubes (132), and the upper end wall of the empty grooves (1312) is provided with unlocking holes (1311).

7. A DNA / RNA synthesizer as defined in claim 6, wherein: The quantitative liquid adding module (14) comprises a valve seat (140), a first valve (143) is arranged on the valve seat (140), the first valve (143) is connected with a liquid inlet connector (141) in a pipeline mode, and a plurality of second valves (144) are connected with the first valve (143) in a pipeline mode, the output ends of the second valves (144) are connected with a third valve (145) in a pipeline mode, and the output end of the third valve (145) is connected with a liquid outlet connector (142) in a pipeline mode; wherein, when liquid is added, the first valve (143) and the second valve (144) are in an open state, and the third valve (145) is in a closed state; when liquid is discharged, the first valve (143) is closed, and the second valve (144) and the corresponding third valve (145) are opened.

Images