A bacteria liquid cleaning device and a cleaning method for bacterial or cell samples

Abstract

The application belongs to the technical field of bacterial liquid cleaning, and particularly relates to a bacterial liquid cleaning device and method for bacterial or cell samples. The device comprises a cleaning well plate, a liquid adding assembly, a liquid sucking assembly, and the bacterial liquid is negatively charged. The liquid adding assembly comprises a liquid adding upper valve block, the liquid adding upper valve block comprises a liquid storage assembly one, and the liquid storage assembly one is connected to a liquid adding assembly one through a liquid adding flow channel. The liquid sucking assembly comprises a liquid sucking upper valve block, the liquid sucking upper valve block is connected to a liquid sucking lower valve block, the liquid sucking lower valve block comprises a liquid storage assembly two, and the liquid storage assembly two is connected to a liquid adding assembly two through a liquid discharging flow channel. The cleaning method comprises the following steps: adding the bacterial liquid into the concave cavity body; adding the cleaning liquid through the liquid adding assembly; sucking the waste liquid through the liquid sucking assembly after the bacterial liquid is cleaned; repeating the cleaning and sucking to obtain the cleaned bacterial liquid; and performing the evaporation drying treatment on the cleaning well plate containing the cleaned bacterial liquid, so as to be transferred to a detection area for cell test detection. The bacterial liquid cleaning device has high efficiency and can improve the accuracy of the cell test.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of bacterial liquid cleaning, and particularly relates to a bacterial liquid cleaning device and method for bacterial or cell samples. BACKGROUND

[0002] In the field of biological medicine, in order to obtain information about molecular composition, molecular structure and molecular interaction in cell tissue, microorganisms such as bacteria, viruses and fungi are separated and purified from culture solution. As a pretreatment means for cell test, the cleaning efficiency, cleaning cleanliness and cell retention rate of bacterial liquid cleaning directly affect the test detection effect. A high-efficiency bacterial liquid cleaning device has important scientific research value and practical significance.

[0003] At present, the common bacterial liquid cleaning means in this research field is centrifugal cleaning. However, centrifugal cleaning needs a lot of manual operation time, and the cleaning process also needs to be based on the experience of manual operation, which can cause problems such as high proportion of cell fragments, low cell viability and low cell retention rate. In addition, repeated centrifugation can also cause uneven loss of cells, thereby affecting the accuracy of the test. SUMMARY

[0004] The first object of the present application is to provide a bacterial liquid cleaning device for bacterial or cell samples, which has high efficiency and can improve the accuracy of cell test.

[0005] A bacterial liquid cleaning device for bacterial or cell samples, the device comprises a cleaning well plate for containing bacterial liquid and cleaning liquid, a liquid adding assembly for adding cleaning liquid into the cleaning well plate, and a liquid suction assembly for sucking away waste liquid in the cleaning well plate, characterized in that the bacterial or cell sample is a cell to be tested, and the bacterial liquid is negatively charged.

[0006] The liquid adding assembly and the liquid suction assembly are symmetrically arranged at two ends of the cleaning well plate. The liquid adding assembly comprises a liquid adding upper valve block, the liquid adding upper valve block comprises a liquid storage assembly one, the liquid storage assembly one is connected with a liquid adding assembly one through a liquid adding flow channel, and the liquid adding assembly one penetrates the liquid adding upper valve block, a liquid adding lower valve block connected with the liquid adding upper valve block in sequence.

[0007] The liquid suction assembly comprises a liquid suction upper valve block, the liquid suction upper valve block is connected with a liquid suction lower valve block, the liquid suction lower valve block comprises a liquid storage assembly two, the liquid storage assembly two is connected with a liquid adding assembly two through a liquid discharging flow channel, and the liquid adding assembly two penetrates the liquid suction upper valve block and the liquid suction lower valve block in sequence.

[0008] Preferably, the liquid storage assembly one comprises a liquid inlet connector, one end of the liquid inlet connector is connected with an external first volume pump, the other end of the liquid inlet connector is connected with a first liquid storage pipe, the first liquid storage pipe is connected with a liquid adding port, and the liquid adding port is connected with the liquid adding flow channel.

[0009] Preferably, the filling assembly one comprises a first piston cavity, a liquid filling piston is arranged in the first piston cavity, and a handle one of the liquid filling piston is outside the first piston cavity;

[0010] The first piston cavity is connected with a liquid filling valve core upper base, and the liquid filling valve core upper base comprises a third flow channel for connecting the first piston cavity.

[0011] Preferably, the liquid filling valve core upper base is connected with a liquid filling valve core sleeve, a second liquid inlet is formed on the liquid filling valve core sleeve, a second through hole is formed on the inner wall of the liquid filling valve core sleeve, and the second liquid inlet is used for connecting the second through hole.

[0012] The upper end of the liquid filling valve core penetrates into the liquid filling valve core sleeve, the liquid filling valve core comprises a hollow liquid filling nozzle, a first liquid inlet is formed on the liquid filling nozzle, and a first sliding rod is connected to the upper part of the liquid filling nozzle.

[0013] Preferably, the filling assembly one further comprises a liquid filling valve core lower base, a first through hole is formed on the inner wall of the liquid filling valve core lower base, and a fourth flow channel is formed on the liquid filling valve core lower base, and the fourth flow channel further connects the liquid filling flow channel.

[0014] The bottom of the liquid filling valve core lower base is provided with a first boss, the first boss is a hollow cylinder with a sealed bottom and a diameter smaller than that of the liquid filling valve core lower base, and an opening cavity one facing the liquid filling valve core lower base is formed in the inside of the first boss, and the first boss is used for providing a lower limit value of the position of the liquid filling valve core when moving.

[0015] The first boss and the fourth flow channel are further provided with a second groove on the liquid filling valve core lower base.

[0016] Preferably, the filling assembly two comprises a second piston cavity, a liquid suction piston is arranged in the second piston cavity, and a handle two of the liquid suction piston is outside the second piston cavity.

[0017] The second piston cavity is connected with a liquid suction valve core upper base, and the liquid suction valve core upper base comprises a sixth flow channel for connecting the second piston cavity.

[0018] Preferably, the liquid suction valve core upper base is connected with a liquid suction valve core sleeve, a second liquid suction port is formed on the liquid suction valve core sleeve, a fourth through hole is formed on the inner wall of the liquid suction valve core sleeve, and the second liquid suction port is used for connecting the fourth through hole.

[0019] The upper end of the liquid suction valve core penetrates into the liquid suction valve core sleeve, the liquid suction valve core comprises a hollow liquid suction nozzle, a first liquid suction port is formed on the liquid suction nozzle, a fifth flow channel is formed on the inner wall of the liquid suction nozzle, and a second sliding rod is connected to the upper part of the liquid suction nozzle.

[0020] Preferably, the filling assembly two further comprises a cylindrical hollow liquid suction valve core lower base, the inner wall of the liquid suction valve core lower base is formed with a third through hole; a seventh flow channel is formed on the liquid suction valve core lower base,

[0021] The bottom of the liquid suction valve core lower base is provided with a second boss, the second boss is a hollow cylinder with a sealed bottom and a diameter smaller than that of the liquid suction valve core lower base, and an open cavity two towards the liquid suction valve core lower base is formed in the interior of the second boss, the second boss is used to provide a lower limit value of the position of the liquid suction valve core during movement,

[0022] The second boss and the seventh flow channel are further provided with a fourth groove on the liquid suction valve core lower base.

[0023] Preferably, the liquid storage assembly two comprises a liquid discharge connector, one end of the liquid discharge connector is connected with an external second volume pump, the second volume pump discharges waste liquid to an external waste liquid collection tank through the liquid discharge connector; the other end of the liquid discharge connector is connected with a second liquid storage pipe, the second liquid storage pipe is connected with the liquid discharge flow channel;

[0024] The washing hole plate comprises a washing seat, the washing seat has a concave cavity body, the concave cavity body comprises a second concave cavity, the second concave cavity is respectively provided with symmetrical first and third concave cavities at two ends, the first and third concave cavities have the same depth, and the depth of the first and third concave cavities is smaller than that of the second concave cavity;

[0025] The surface of the concave cavity body is further provided with a functional layer of a non-specific cell adhesion factor, the non-specific cell adhesion factor is polylysine, and the non-specific cell adhesion factor forms a coating on the concave cavity body by coating or chemical plating.

[0026] By using the above technical scheme, polylysine is a positively charged amino acid polymer, which can be combined with DNA, red blood cell membranes or any negatively charged protein, and can enhance the electrostatic interaction between the negative charge ions on the cell membrane surface and the solid matrix surface (such as a cell culture dish, a glass slide, etc.), so that the functional layer is positively charged, the positively charged functional layer and the negatively charged bacterial liquid can interact, and the suspended cells in the bacterial liquid can be accelerated to settle at the bottom of the concave cavity body.

[0027] Another object of the present application is to provide a washing method, the bacterial liquid containing bacterial or cell samples is washed by using the bacterial liquid washing device for bacterial or cell samples,

[0028] The washing method comprises the following steps:

[0029] S1. A certain amount of the bacterial liquid is added into the concave cavity body;

[0030] S2. The liquid adding assembly adds cleaning liquid into the concave cavity body;

[0031] S3. After the bacterial liquid is cleaned, the liquid sucking assembly sucks waste liquid from the concave cavity body;

[0032] S4. Steps S2 and S3 are repeated to clean the bacterial liquid multiple times to obtain cleaned bacterial liquid;

[0033] S5. The cleaned bacterial liquid in the cleaning well plate is subjected to evaporation drying treatment and is transferred to a detection area for cell test detection.

[0034] According to the technical scheme, the liquid adding nozzle adds cleaning liquid into the bacterial liquid to dilute the bacterial liquid, and the laminar flow cleaning liquid added is used to repeatedly blow the sediment in the concave cavity body to make the impurities at the bottom suspended again, so that the target cells are kept to continue to settle at the bottom of the concave cavity body. At this time, the liquid sucking nozzle is used to suck the suspended waste liquid in the concave cavity body to reduce the content of the impurities in the bacterial liquid. The adding and sucking processes are repeatedly performed to continuously reduce the impurities in the bacterial liquid until the target cells, i.e. the cleaned bacterial liquid, are obtained.

[0035] The beneficial effects of the present application are as follows:

[0036] Firstly, the device has high efficiency. Specifically, the surface of the concave cavity body is further provided with a functional layer of non-specific cell adhesion factor. The non-specific cell adhesion factor is polylysine. The non-specific cell adhesion factor forms a coating layer on the concave cavity body by coating or chemical plating. The polylysine is a positively charged amino acid polymer that can be combined with DNA, red blood cell membranes or any negatively charged protein, and can enhance the electrostatic interaction between the negative charge ions on the cell membrane surface and the solid matrix surface (such as cell culture dishes, glass slides, etc.). Therefore, the functional layer is positively charged due to the polylysine, and the positively charged functional layer and the negatively charged bacterial liquid can interact with each other to accelerate the sedimentation of the suspended cells in the bacterial liquid to the bottom of the concave cavity body. In addition, the liquid adding assembly, the liquid sucking assembly and the cleaning well plate of the device are separately arranged, which is convenient to operate and has high coordination. The cooperation of the first sliding rod, the first base hole, the first spring and the liquid adding valve core sleeve can provide an upper limit position for the upward sliding of the liquid adding valve core. The cooperation of the second sliding rod, the second base hole, the third spring and the liquid sucking valve core sleeve can provide an upper limit position for the upward sliding of the liquid sucking valve core. The cooperation of the second spring and the first boss can provide a lower limit position for the downward sliding of the liquid adding valve core. The cooperation of the fourth spring and the second boss can provide a lower limit position for the downward sliding of the liquid sucking valve core.

[0037] Secondly, the device of the application reduces the proportion of cell fragments, improves cell viability and retention rate by using laminar flow cleaning method, thereby increasing the accuracy of cell test. Specifically, the liquid injection nozzle injects cleaning liquid into the bacterial liquid to dilute the bacterial liquid. At the same time, the laminar flow cleaning liquid repeatedly blows the sediment in the cavity body to make the impurities in the bottom suspended again, and the larger target cells are retained to continue to settle at the bottom of the cavity body. At this time, the liquid suction nozzle reduces the content of impurities in the bacterial liquid by sucking the suspended waste liquid in the cavity body. The injection and suction process is repeated to continuously reduce the impurities in the bacterial liquid until the target cells are obtained, that is, the cleaned bacterial liquid.

[0038] Thirdly, the device of the application is easy to control the speed. Specifically, the liquid injection piston is driven by a mechanical driving mechanism to make reciprocating motion to inject cleaning liquid into the cavity body of the cleaning hole plate. The non-linear motion of the liquid injection piston is controlled to control the intermittent change of the liquid injection speed to blow the bacterial liquid. After the bacterial liquid is cleaned, the liquid suction assembly sucks the waste liquid from the cavity body. The liquid suction piston is driven by a mechanical driving mechanism to make reciprocating motion to suck the waste liquid from the cavity body of the cleaning hole plate. The motion speed of the liquid suction piston is controlled to control the liquid suction speed to avoid the suction of cells.

[0039] Fourthly, the device of the application has good sealing performance. Specifically, the device of the application is provided with a plurality of sealing components, including a first rectangular sealing ring, a second rectangular sealing ring, a first O-shaped sealing ring, a second O-shaped sealing ring, a third O-shaped sealing ring, a fourth O-shaped sealing ring, a first screw, and a second screw.

[0040] In summary, the bacterial liquid cleaning device of the application has high efficiency and can improve the accuracy of cell test. BRIEF DESCRIPTION OF DRAWINGS

[0041] The accompanying drawings are included to provide a further understanding of the application, and constitute a part of the specification, illustrate the application together with the embodiments thereof, and explain the application without limiting the application.

[0042] In the drawings:

[0043] Figure 1 It is a schematic diagram of the three-dimensional structure of the device of the application;

[0044] Figure 2 It is a schematic diagram of the bottom three-dimensional structure of the device of the application;

[0045] Figure 3 It is a sectional view of the structure of the device of the application from the middle section;

[0046] Figure 4 It is an enlarged schematic diagram of the middle A part of the device of the application; Figure 3

[0047] ​Figure 5 Fig. 3 is a perspective view of the upper base 15 of the liquid adding valve core according to the present application;

[0048] Figure 6 Fig. 4 is a sectional view of the upper base 15 of the liquid adding valve core according to the present application, taken along the middle section thereof;

[0049] Figure 7 Fig. 5 is a sectional view of the upper base 25 of the liquid sucking valve core according to the present application, taken along the middle section thereof;

[0050] Figure 8 Fig. 6 is a perspective view of the lower base 16 of the liquid adding valve core according to the present application (with the first boss 165 removed);

[0051] Figure 9 Fig. 7 is a sectional view of the lower base 16 of the liquid adding valve core according to the present application, taken along the middle section thereof (with the first boss 165 removed);

[0052] Figure 10 Fig. 8 is a sectional view of the lower base 26 of the liquid sucking valve core according to the present application, taken along the middle section thereof (with the second boss 265 removed);

[0053] Figure 11 Fig. 9 is a perspective view of the sleeve 17 of the liquid adding valve core according to the present application;

[0054] Figure 12 Fig. 10 is a sectional view of the sleeve 17 of the liquid adding valve core according to the present application, taken along the middle section thereof;

[0055] Figure 13 Fig. 11 is a perspective view of the sleeve 27 of the liquid sucking valve core according to the present application;

[0056] Figure 14 Fig. 12 is a sectional view of the sleeve 27 of the liquid sucking valve core according to the present application, taken along the middle section thereof;

[0057] Figure 15 Fig. 13 is a perspective view of the liquid adding valve core 14 according to the present application;

[0058] Figure 16 Fig. 14 is a sectional view of the liquid adding valve core 14 according to the present application, taken along the middle section thereof;

[0059] Figure 17 Fig. 15 is a perspective view of the liquid sucking valve core 24 according to the present application;

[0060] Figure 18 Fig. 16 is a sectional view of the liquid sucking valve core 24 according to the present application, taken along the middle section thereof;

[0061] Figure 19 Fig. 17 is a flowchart of the cleaning method according to the present application;

[0062] Marked in the figure: 10, liquid adding assembly; 11, liquid adding piston; 12, upper liquid adding valve block; 121, liquid inlet joint; 122, first piston cavity; 123, first liquid storage pipe; 124, liquid adding port; 13, lower liquid adding valve block; 131, first screw; 132, liquid adding flow channel; 133, first rectangular sealing ring; 14, liquid adding valve core; 141, liquid adding nozzle; 142, first O-shaped sealing ring; 143, first flow channel; 144, first sliding rod; 145, first liquid inlet; 146, second flow channel; 147, first liquid adding sliding block; 148, second liquid adding sliding block; 149, first groove; 15, upper base of liquid adding valve core; 151, third flow channel; 152, first base end face; 153, first base hole; 16, lower base of liquid adding valve core; 161, first through hole; 162, second O-shaped sealing ring; 163, fourth flow channel; 164, second groove; 165, first boss; 17, sleeve of liquid adding valve core; 171, second liquid inlet; 172, second through hole; 18, first spring; 19, second spring;

[0063] 20, liquid suction assembly; 21, liquid suction piston; 22, upper liquid suction valve block; 221, second piston cavity; 23, lower liquid suction valve block; 231, second screw; 232, liquid outlet joint; 233, liquid outlet flow channel; 234, second liquid storage pipe; 235, second rectangular sealing ring; 24, liquid suction valve core; 241, liquid suction nozzle; 242, third O-shaped sealing ring; 243, fifth flow channel; 244, second sliding rod; 245, first liquid suction port; 246, liquid suction sliding block; 247, third groove; 25, upper base of liquid suction valve core; 251, sixth flow channel; 252, second base end face; 253, second base hole; 26, lower base of liquid suction valve core; 261, third through hole; 262, fourth O-shaped sealing ring; 263, seventh flow channel; 264, fourth groove; 265, second boss; 27, sleeve of liquid suction valve core; 271, second liquid suction port; 272, fourth through hole; 28, third spring; 29, fourth spring;

[0064] 30, cleaning hole plate; 31, first concave cavity; 32, second concave cavity; 33, cleaning seat; 34, third concave cavity. DETAILED DESCRIPTION

[0065] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0066] Embodiment 1

[0067] As Figures 1 to 18As shown, a bacteria liquid washing device for bacterial or cell sample,

[0068] The bacteria liquid washing device comprises a washing well plate 30, preferably the washing well plate 30 is a disposable consumable, the washing well plate 30 is used for containing a bacteria liquid and a washing liquid, the bacteria liquid is negatively charged; the washing well plate 30 comprises a washing seat 33, the washing seat 33 has a concave cavity body, the concave cavity body comprises a second concave cavity 32, the second concave cavity 32 is respectively provided with symmetrical first concave cavities 31 and third concave cavities 34 at two ends, the depths of the first concave cavities 31 and the third concave cavities 34 are the same, and the depths of the first concave cavities 31 and the third concave cavities 34 are smaller than the depth of the second concave cavity 32.

[0069] The surface of the concave cavity body is further provided with a functional layer of a non-specific cell adhesion factor, preferably the non-specific cell adhesion factor is poly-D-lysine or poly-L-lysine, the non-specific cell adhesion factor is coated or chemically plated on the concave cavity body to form a coating layer, the poly-D-lysine and the poly-L-lysine are both polylysine, which is a positively charged amino acid polymer, can be combined with DNA, red blood cell membranes or any negatively charged protein, and can enhance the electrostatic interaction between the negative charge ions on the surface of the cell membrane and the surface of the solid matrix (such as a cell culture dish, a glass slide, etc.), so that the functional layer is positively charged due to the poly-D-lysine or the poly-L-lysine, and the positively charged functional layer and the negatively charged bacteria liquid can interact with each other, so that the suspended cells in the bacteria liquid are accelerated to settle at the bottom of the concave cavity body.

[0070] The two ends of the washing well plate 30 are symmetrically provided with sealed liquid adding assemblies 10 and liquid suction assemblies 20, the liquid adding assembly 10 comprises a liquid adding upper valve block 12, the liquid adding upper valve block 12 comprises a liquid storage assembly one, the liquid storage assembly one comprises a liquid inlet connector 121, one end of the liquid inlet connector 121 is connected with an external first volume pump, the other end of the liquid inlet connector 121 is connected with a vertical first liquid storage pipe 123, and the first liquid storage pipe 123 is used for buffering the washing liquid.

[0071] The other end of the first liquid storage pipe 123 is connected with a vertical liquid adding port 124, and the liquid adding port 124 is connected with a liquid adding flow channel 132.

[0072] The liquid adding flow channel 132 is communicated with a liquid adding assembly one, the liquid adding assembly one penetrates the liquid adding upper valve block 12 and a liquid adding lower valve block 13 in sequence, the liquid adding lower valve block 13 is connected with the liquid adding upper valve block 12 through a first screw 131 installed at the bottom of the liquid adding lower valve block 13, preferably the number of the first screw 131 is six, there is a first rectangular sealing ring 133 between the liquid adding upper valve block 12 and the liquid adding lower valve block 13, and the first rectangular sealing ring 133 is used for sealing the liquid adding upper valve block 12 and the liquid adding lower valve block 13.

[0073] The filling assembly one includes a first piston cavity 122, a liquid filling piston 11 is arranged in the first piston cavity 122, a handle one of the liquid filling piston 11 is outside the first piston cavity 122, the first piston cavity 122 is connected with a liquid filling valve core upper base 15, the liquid filling valve core upper base 15 includes vertical third flow channels 151, preferably the number of the third flow channels 151 is four, the third flow channels 151 are used for connecting the first piston cavity 122, a cylindrical first base end face 152 is formed in the liquid filling valve core upper base 15, a hollow first base hole 153 is formed on the first base end face 152.

[0074] The liquid filling valve core upper base 15 is connected with a liquid filling valve core sleeve 17, a second liquid inlet 171 is formed on the liquid filling valve core sleeve 17, a second through hole 172 is formed on the inner wall of the liquid filling valve core sleeve 17, the second liquid inlet 171 is used for connecting the second through hole 172.

[0075] The liquid filling valve core 14 is penetrated into the liquid filling valve core sleeve 17, the liquid filling valve core 14 includes a vertical hollow liquid filling nozzle 141, the liquid filling nozzle 141 has vertical first flow channels 143, a first liquid inlet 145 is formed on the liquid filling nozzle 141, a hollow cylindrical first liquid filling slider 147 is arranged on the liquid filling nozzle 141 below the first liquid inlet 145, a second flow channel 146 is formed on the first liquid filling slider 147, preferably the number of the second flow channel 146 is four; a hollow cylindrical second liquid filling slider 148 is arranged on the liquid filling nozzle 141 below the first liquid filling slider 147, the first liquid filling slider 147 and the second liquid filling slider 148 have high coaxiality.

[0076] The second liquid filling slider 148 has a first recess 149, there is a cylindrical gap between the first liquid filling slider 147 and the second liquid filling slider 148, the gap is a passage one, the passage one is used for connecting the liquid filling flow channel 132 when needed.

[0077] The liquid filling nozzle 141 is connected with a first sliding rod 144, the first sliding rod 144 is an inverted T shape, the inverted T shape includes a horizontal rod one and a vertical rod one which are perpendicular to each other, the horizontal rod one can slide in the liquid filling valve core sleeve 17, the vertical rod one is penetrated into the first base hole 153 and can slide in the first base hole 153, a first spring 18 is arranged between the liquid filling valve core sleeve 17 and the first liquid filling slider 147, the cooperation of the first sliding rod 144, the first base hole 153, the first spring 18 and the liquid filling valve core sleeve 17 can provide an upper limit position for the upward sliding of the liquid filling valve core 14.

[0078] The filling assembly one further comprises a cylindrical hollow liquid filling valve core lower base 16, the inner wall of the liquid filling valve core lower base 16 is formed with a vertical first through hole 161, the liquid filling valve core lower base 16 is formed with a fourth flow channel 163, the fourth flow channel 163 is communicated with a liquid filling flow channel 132; the lower side of the fourth flow channel 163 is provided with a first boss 165 arranged at the bottom of the liquid filling valve core lower base 16, the first boss 165 is a hollow cylinder with a sealed bottom and a diameter smaller than that of the liquid filling valve core lower base 16, and an opening cavity one towards the liquid filling valve core lower base 16 is formed in the interior of the first boss 165, between the first boss 165 and the fourth flow channel 163, a second groove 164 is arranged on the liquid filling valve core lower base 16, a ring-shaped gap one for placing a second O-shaped sealing ring 162 is arranged in the middle of the second groove 164, and the second O-shaped sealing ring 162 is used for sealing the liquid filling valve core lower base 16 and the liquid filling lower valve block 13 to prevent liquid leakage.

[0079] The first liquid filling sliding block 147 and the second liquid filling sliding block 148 can slide in the first through hole 161, the liquid filling nozzle 141 penetrates into the first boss 165, a second spring 19 is arranged between the bottom of the second liquid filling sliding block 148 and the bottom of the first boss 165, the second spring 19 cooperates with the first boss 165 to provide a lower limit position for the downward sliding of the liquid filling valve core 14; in addition, the first O-shaped sealing ring 142 is arranged in the first groove 149 to seal the liquid filling valve core lower base 16 and the liquid filling valve core 14.

[0080] The liquid suction assembly 20 comprises a liquid suction lower valve block 23, the liquid suction lower valve block 23 comprises a second liquid storage assembly, the second liquid storage assembly comprises a liquid discharge connector 232 arranged at the bottom of the liquid suction lower valve block 23, the liquid discharge connector 232 is connected with a second liquid storage pipe 234 in the interior of the liquid suction lower valve block 23, the second liquid storage pipe 234 is connected with the filling assembly two through a liquid discharge flow channel 233, the filling assembly two penetrates into the liquid suction upper valve block 22 and the liquid suction lower valve block 23 in sequence, the liquid suction lower valve block 23 is connected with the liquid suction upper valve block 22 through a second screw 231 arranged at the bottom of the liquid suction lower valve block 23, preferably, the number of the second screw 231 is six, and there is a second rectangular sealing ring 235 between the liquid suction lower valve block 23 and the liquid suction upper valve block 22, the second rectangular sealing ring 235 is used for sealing the liquid suction lower valve block 23 and the liquid suction upper valve block 22.

[0081] The filling assembly two comprises a second piston cavity 221, the second piston cavity 221 is provided with a liquid suction piston 21, a handle two of the liquid suction piston 21 is arranged outside the second piston cavity 221, the second piston cavity 221 is connected with a liquid suction valve core upper base 25, the liquid suction valve core upper base 25 comprises vertical sixth flow channels 251, preferably, the number of the sixth flow channels 251 is six, and the sixth flow channels 251 are used for communicating the second piston cavity 221, the liquid suction valve core upper base 25 is formed with a cylindrical second base end face 252 in the interior, and the second base end face 252 is formed with a hollow second base hole 253.

[0082] The suction valve core upper base 25 is connected with a suction valve core sleeve 27, and the suction valve core sleeve 27 is formed with a second suction port 271, and the inner wall of the suction valve core sleeve 27 is formed with a vertical fourth through hole 272, and the second suction port 271 is used for communicating the fourth through hole 272.

[0083] The suction valve core 24 upper end penetrates into the suction valve core sleeve 27, and the suction valve core 24 includes a vertical hollow suction nozzle 241, and the suction nozzle 241 is internally provided with a vertical fifth flow channel 243, and the suction nozzle 241 is formed with a first suction port 245, and the first suction port 245 is provided with a hollow cylindrical suction sliding block 246 on the suction nozzle 241.

[0084] The suction nozzle 241 upper portion is connected with a second sliding rod 244, and the second sliding rod 244 is an inverted T-shaped structure, and the inverted T-shaped structure includes a horizontal rod two and a vertical rod two which are perpendicular to each other, the horizontal rod two can slide in the suction valve core sleeve 27, the vertical rod two penetrates into the second base hole 253 and can slide in the second base hole 253, and a third spring 28 is arranged between the suction valve core sleeve 27 and the suction sliding block 246, and the cooperation of the second sliding rod 244, the second base hole 253, the third spring 28 and the suction valve core sleeve 27 can provide an upper limit position for the upward sliding of the suction valve core 24.

[0085] The filling assembly two further includes a cylindrical hollow suction valve core lower base 26, and the inner wall of the suction valve core lower base 26 is formed with a vertical third through hole 261; the suction valve core lower base 26 is formed with a seventh flow channel 263, and the seventh flow channel 263 is provided with a second boss 265 arranged at the bottom of the suction valve core lower base 26, the second boss 265 is a hollow cylinder with a sealed bottom and a diameter smaller than that of the suction valve core lower base 26, and an opening cavity two is formed inside the second boss 265 and faces the suction valve core lower base 26, and a fourth groove 264 is arranged on the suction valve core lower base 26 between the second boss 265 and the seventh flow channel 263, and the fourth groove 264 is provided with a ring-shaped gap two in the middle for placing a fourth O-shaped sealing ring 262, and the fourth O-shaped sealing ring 262 is used for sealing the suction valve core lower base 26 and the suction lower valve block 23 to prevent liquid leakage.

[0086] The suction sliding block 246 can slide in the third through hole 261, the suction nozzle 241 penetrates into the second boss 265, and a fourth spring 29 is arranged between the bottom of the suction sliding block 246 and the bottom of the second boss 265, and the fourth spring 29 cooperates with the second boss 265 to provide a lower limit position for the downward sliding of the suction valve core 24, and the suction sliding block 246 is further formed with a third groove 247, and a third O-shaped sealing ring 242 is arranged in the third groove 247 and is used for sealing the suction valve core lower base 26 and the suction valve core 24.

[0087] Example 2

[0088] As Figure 19As shown, a cleaning method is used to clean the bacterial or cell sample-containing bacterial liquid by using the bacterial liquid cleaning device for bacterial or cell sample of Example 1, the bacterial or cell sample is the cell to be tested, and the bacterial liquid is negatively charged.

[0089] The cleaning method comprises the following steps:

[0090] S1. Add the bacterial liquid to the concave cavity body of the cleaning hole plate 30 in a certain amount, and extend the liquid adding nozzle 141 of the liquid adding assembly 10 and the liquid sucking nozzle 241 of the liquid sucking assembly 20 into the corresponding parts of the concave cavity body, and the liquid adding nozzle 141 and the liquid sucking nozzle 241 are immersed in the bacterial liquid at a suitable position;

[0091] S2. The liquid adding assembly 10 adds the cleaning liquid to the concave cavity body:

[0092] S21. Pump the cleaning liquid into the first liquid storage pipe 123 through the external first volume pump, and keep the liquid pressure in the first liquid storage pipe 123 within a preset range;

[0093] S22. Drive the liquid adding piston 11 to reciprocate by the mechanical driving mechanism, so as to add the cleaning liquid to the concave cavity body of the cleaning hole plate 30; control the intermittent change of the liquid adding speed by controlling the nonlinear motion of the liquid adding piston 11, so as to blow the bacterial liquid;

[0094] S3. After the bacterial liquid is cleaned, the liquid sucking assembly 20 sucks the waste liquid from the concave cavity body: drive the liquid sucking piston 21 to reciprocate by the mechanical driving mechanism, so as to suck the waste liquid from the concave cavity body of the cleaning hole plate 30; control the liquid sucking speed by controlling the motion speed of the liquid sucking piston 21, so as to avoid the suction of the cells;

[0095] S4. Repeat steps S2 and S3 to clean the bacterial liquid multiple times to obtain the cleaned bacterial liquid;

[0096] S5. Perform the evaporation drying treatment on the cleaning hole plate 30 containing the cleaned bacterial liquid, so as to be transferred to the detection area for cell test detection.

[0097] In combination with Example 1 and Example 2, the bacterial liquid cleaning device for bacterial or cell sample proposed in the application, in combination with the cleaning method, the working process is as follows:

[0098] S1. Add the bacterial liquid to the concave cavity body (specifically the first concave cavity 31, the second concave cavity 32, and the third concave cavity 34) of the cleaning hole plate 30 in a certain amount, extend the liquid adding nozzle 141 of the liquid adding assembly 10 into the first concave cavity 31 and immerse it in the bacterial liquid at a suitable position, and extend the liquid sucking nozzle 241 of the liquid sucking assembly 20 into the third concave cavity 34 and immerse it in the bacterial liquid at a suitable position;

[0099] The bacterial solution carries a negative charge, while the functional layer of the concave cavity carries a positive charge. This accelerates the sedimentation process of the negatively charged target cells in the suspended bacterial solution, greatly reducing the bacterial solution washing time.

[0100] S2. The liquid filling assembly 10 adds cleaning fluid into the concave cavity body:

[0101] S21. The first external volumetric pump connected to the inlet connector 121 pumps cleaning fluid into the first storage tube 123 and keeps the hydraulic pressure in the first storage tube 123 within a preset range. The first storage tube 123 buffers the cleaning fluid. The cleaning fluid flows from the first storage tube 123 into the filling channel 132 through the filling port 124. The cleaning fluid enters the fourth channel 163 of the lower base 16 of the filling valve core through the filling channel 132.

[0102] S22. The initial position of the liquid filling piston 11 is at the bottom of the first piston chamber 122 and in contact with the base 15 on the liquid filling valve core. At this time, the second liquid filling slider 148 moves closer to the first boss 165, and the liquid filling valve core 14 is at the limit position of the downward stroke. The cleaning fluid flows into the second flow channel 146 from the fourth flow channel 163 and flows into the first through hole 161 and the third flow channel 151 in sequence from the second flow channel 146. At this time, the crossbar of the first slide rod 144 is in contact with the second liquid inlet 171, and the passage between the second liquid inlet 171 and the first liquid inlet 145 is closed.

[0103] When needed, the external mechanical drive mechanism (preferably motor) connected to the liquid adding piston 11 is activated. The motor drives the liquid adding piston 11 to move upward in the first piston chamber 122. Under the action of negative pressure, the liquid adding valve core 14 also moves upward. During the upward movement, the passages of the second flow channel 146 and the fourth flow channel 163 will be closed first, and the first liquid inlet 145 will contact the second liquid inlet 171. When they contact, the passages are opened, and a portion of the cleaning solution will first enter the first flow channel 143. The cleaning solution is then injected into the first concave cavity 31 of the cleaning orifice plate 30 through the liquid adding nozzle 141 via the first flow channel 143 to dilute and clean the bacterial solution.

[0104] As the filling piston 11 continues to move upward, the passage between the first inlet 145 and the second inlet 171 will be closed. When it moves to the top of the first piston chamber 122, the filling valve core 14 moves to the top, that is, the horizontal bar of the first slide rod 144 slides upward to the top of the filling valve core sleeve 17, and the vertical bar of the first slide rod 144 slides upward to the top of the first base hole 153. The cooperation of the first slide rod 144, the first base hole 153, the first spring 18, and the filling valve core sleeve 17 provides the upper limit of the sliding position of the filling valve core 14.

[0105] At this time, the passage between the fourth flow channel 163 and the second flow channel 146 is open, and the cleaning liquid flows from the fourth flow channel 163 into the second flow channel 146 through the passage, and then into the first through hole 161. The cleaning liquid in the first through hole 161 is pushed upward by the liquid adding valve core 14 into the first piston cavity 122;

[0106] During the upward movement of the liquid adding valve core 14, a part of the cleaning liquid is first pushed upward by the liquid adding valve core 14 into the first piston cavity 122 for buffering and storage;

[0107] Then, the motor one drives the liquid adding piston 11 to move downward in the first piston cavity 122. Under the action of positive pressure, the liquid adding valve core 14 also moves downward. During the downward movement, the passage between the fourth flow channel 163 and the second flow channel 146 is first closed, and the first liquid inlet 145 will contact the second liquid inlet 171. When the two are in contact, the passage is open, and a part of the cleaning liquid will enter the first flow channel 143. The cleaning liquid is added into the first recess 31 of the cleaning hole plate 30 through the first flow channel 143 and the liquid adding nozzle 141, and the bacterial liquid is diluted and cleaned;

[0108] When the liquid adding piston 11 continues to move downward, the passage between the first liquid inlet 145 and the second liquid inlet 171 is closed. When the liquid adding valve core 14 moves downward to the bottom end of the first piston cavity 122, i.e., returns to the initial position, i.e., the horizontal rod one of the first slide rod 144 slides downward to the lowermost end of the liquid adding valve core sleeve 17, and the vertical rod one of the first slide rod 144 slides downward to the lowermost end of the first base hole 153, the second spring 19 cooperates with the first boss 165 to provide the lower limit value of the sliding position of the liquid adding valve core 14;

[0109] The motor one (mechanical driving mechanism one) drives the liquid adding piston 11 to make nonlinear movement, so as to control the intermittent change of the liquid adding speed, thereby repeatedly blowing and washing the bacterial liquid.

[0110] S3. After the bacterial liquid is cleaned, the liquid suction assembly 20 sucks the waste liquid from the recess body:

[0111] The initial position of the liquid suction piston 21 is at the bottom of the second piston cavity 221, and is in contact with the upper base 25 of the liquid suction valve core. At this time, the liquid suction slide block 246 approaches the second boss 265, the liquid suction valve core 24 is at the limit position of the downward stroke, the first liquid suction port 245 is not in contact with the second liquid suction port 271, and the passage is in a closed state;

[0112] The second external mechanical driving mechanism (preferably the second motor) connected to the suction piston 21 is started to drive the suction piston 21 to move upward in the second piston cavity 221, and under the action of negative pressure, the suction valve core 24 also moves upward, and in the process of upward movement, the waste liquid in the third recessed cavity 34 is sucked into the suction nozzle 241, the fifth flow channel 243, and continues to move upward, when the suction piston 21 moves to the top end of the second piston cavity 221, the suction valve core 24 moves to the uppermost end, that is, the horizontal rod two of the second slide rod 244 slides upward to the top end of the suction valve core sleeve 27, and the vertical rod two of the second slide rod 244 slides upward to the top end of the second base hole 253, and the cooperation of the second slide rod 244, the second base hole 253, the third spring 28 and the suction valve core sleeve 27 provides an upper limit value of the sliding position of the suction valve core 24;

[0113] At this time, the third through hole 261 is not communicated with the seventh flow channel 263, the first suction port 245 is in contact with the second suction port 271, and the waste liquid is sequentially sucked from the fifth flow channel 243 into the first suction port 245 and the second suction port 271, and then sequentially enters the third through hole 261, the sixth flow channel 251 and the second piston cavity 221,

[0114] Then the second motor is used to drive the suction piston 21 to move downward in the second piston cavity 221, and under the action of positive pressure, the suction valve core 24 also moves downward, and in the process of downward movement, the first suction port 245 stops contacting with the second suction port 271, and the passage is closed,

[0115] When the suction piston 21 continues to move downward, the third through hole 261 is communicated with the seventh flow channel 263, the waste liquid in the third through hole 261 flows into the liquid discharge flow channel 233 from the seventh flow channel 263, and then sequentially flows into the second liquid storage pipe 234, and the second volume pump connected to the liquid discharge connector 232 discharges the waste liquid in the second liquid storage pipe 234 into the external waste liquid collection tank through the liquid discharge connector 232.

[0116] The second motor (the second mechanical driving mechanism) drives the suction piston 21 to make nonlinear motion to control the intermittent change of the liquid adding speed, so as to suck the waste liquid, and also to avoid the suction of cells by controlling the suction speed.

[0117] S4. Repeat steps S2 and S3 to wash the bacterial liquid multiple times to obtain the washed bacterial liquid: the liquid adding nozzle 141 adds the washing liquid to the bacterial liquid to dilute the bacterial liquid, and at the same time, the laminar flow washing liquid added repeatedly blows and washes the sediment in the cavity body to make the impurities at the bottom suspended again, and the larger target cells are retained to continue to settle at the bottom of the cavity body; at this time, the suction nozzle 241 reduces the content of impurities in the bacterial liquid by sucking the suspended waste liquid in the cavity body; the adding and sucking process is repeatedly performed to continuously reduce the impurities in the bacterial liquid, until the target cells are relatively clean, that is, the washed bacterial liquid.

[0118] S5. The washing well plate 30 containing the washed bacteria solution is evaporated to dryness, and is ready to be transferred to the detection area for cell test detection.

[0119] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art will appreciate that the technical solutions described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalent features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A bacterial or cell sample washing device for bacterial liquid, the device comprising a washing well plate (30) for containing bacterial liquid and washing liquid, a liquid adding assembly (10) for adding washing liquid into the washing well plate (30), a liquid suction assembly (20) for sucking away waste liquid in the washing well plate (30), characterized in that, The bacterial or cell sample is a cell to be tested, and the bacterial solution is negatively charged; The liquid adding assembly (10) and the liquid sucking assembly (20) are symmetrically arranged at two ends of the washing hole plate (30), the liquid adding assembly (10) comprises a liquid adding upper valve block (12), the liquid adding upper valve block (12) comprises a liquid storage assembly one, the liquid storage assembly one is communicated with a liquid adding filling assembly one through a liquid adding flow channel (132), and the liquid adding filling assembly one is sequentially inserted into the liquid adding upper valve block (12) and a liquid adding lower valve block (13) connected with the liquid adding upper valve block (12); The liquid sucking assembly (20) comprises a liquid sucking upper valve block (22), the liquid sucking upper valve block (22) is connected with a liquid sucking lower valve block (23), the liquid sucking lower valve block (23) comprises a liquid storage assembly two, the liquid storage assembly two is communicated with a liquid sucking filling assembly two through a liquid discharging flow channel (233), and the liquid sucking filling assembly two is sequentially inserted into the liquid sucking upper valve block (22) and the liquid sucking lower valve block (23), and the liquid sucking filling assembly two comprises a second piston cavity (221); The liquid adding filling assembly one comprises a first piston cavity (122), a liquid adding piston (11) is arranged in the first piston cavity (122), and a handle one of the liquid adding piston (11) is arranged outside the first piston cavity (122); The first piston cavity (122) is connected with a liquid adding valve core upper base (15), the liquid adding valve core upper base (15) comprises a third flow channel (151), and the third flow channel (151) is used for communicating the first piston cavity (122); The liquid adding valve core upper base (15) is connected with a liquid adding valve core sleeve (17), a second liquid inlet (171) is formed in the liquid adding valve core sleeve (17), a second through hole (172) is formed in an inner wall of the liquid adding valve core sleeve (17), and the second liquid inlet (171) is used for communicating the second through hole (172); A liquid adding nozzle (141) of the liquid adding valve core (14) is hollow, a first liquid inlet (145) is formed in the liquid adding nozzle (141), and a first sliding rod (144) is connected to an upper portion of the liquid adding nozzle (141); The liquid adding filling assembly one further comprises a liquid adding valve core lower base (16), a first through hole (161) is formed in an inner wall of the liquid adding valve core lower base (16), and a fourth flow channel (163) is formed in the liquid adding valve core lower base (16) and further communicates with the liquid adding flow channel (132); A first boss (165) is arranged at a bottom of the liquid adding valve core lower base (16), the first boss (165) is a hollow cylinder with a sealed bottom and a diameter smaller than that of the liquid adding valve core lower base (16), an opening cavity one is formed in an inner portion of the first boss (165) and faces the liquid adding valve core lower base (16), and the first boss (165) is used for providing a lower limit value of a position of the liquid adding valve core (14) during movement; A second groove (164) is further arranged on the liquid adding valve core lower base (16) between the first boss (165) and the fourth flow channel (163).

2. The bacterial or cell sample washing device of claim 1, wherein, The liquid storage assembly one includes a liquid inlet connector (121), one end of which is connected with an external first volume pump, and the other end of which is connected with a first liquid storage pipe (123), which is connected with a liquid filling port (124), and the liquid filling port (124) is connected with the liquid filling flow channel (132).

3. The bacterial or cell sample washing device of claim 1, wherein, The second piston cavity (221) is provided with a liquid suction piston (21), and a handle of the liquid suction piston (21) is outside the second piston cavity (221). The second piston cavity (221) is connected with a liquid suction valve core upper base (25), and the liquid suction valve core upper base (25) includes a sixth flow channel (251) for connecting the second piston cavity (221).

4. The bacterial or cell sample washing device of claim 3, wherein, The liquid suction valve core upper base (25) is connected with a liquid suction valve core sleeve (27), the liquid suction valve core sleeve (27) is formed with a second liquid suction port (271), and an inner wall of the liquid suction valve core sleeve (27) is formed with a fourth through hole (272), and the second liquid suction port (271) is used for connecting the fourth through hole (272). The liquid suction valve core (24) is inserted into the liquid suction valve core sleeve (27) at an upper end, and the liquid suction valve core (24) includes a hollow liquid suction nozzle (241), the liquid suction nozzle (241) is formed with a first liquid suction port (245), an inner wall of the liquid suction nozzle (241) is formed with a fifth flow channel (243), and the liquid suction nozzle (241) is connected with a second sliding rod (244) at an upper portion.

5. The bacterial or cell sample washing device of claim 4, wherein, The liquid filling assembly two further includes a cylindrical hollow liquid suction valve core lower base (26), and an inner wall of the liquid suction valve core lower base (26) is formed with a third through hole (261); the liquid suction valve core lower base (26) is formed with a seventh flow channel (263), The liquid suction valve core lower base (26) is provided with a second boss (265), which is a hollow cylinder with a sealed bottom and a diameter smaller than that of the liquid suction valve core lower base (26), and is internally formed with a second opening cavity facing the liquid suction valve core lower base (26), and the second boss (265) is used for providing a lower limit value of the position of the liquid suction valve core (24) during movement, The second boss (265) and the seventh flow channel (263) are further provided with a fourth groove (264) on the liquid suction valve core lower base (26).

6. The bacterial or cell sample washing device of claim 1, wherein, The liquid storage assembly two includes a liquid discharge connector (232), one end of which is connected with an external second volume pump, and the other end of which is connected with a second liquid storage pipe (234), and the second liquid storage pipe (234) is connected with the liquid discharge flow channel (233). The cleaning hole plate (30) comprises a cleaning seat (33) with a concave cavity body, the concave cavity body comprises a second concave cavity (32), the two ends of the second concave cavity (32) are respectively provided with symmetrical first concave cavities (31) and third concave cavities (34), the depths of the first concave cavities (31) and the third concave cavities (34) are the same, and are smaller than the depth of the second concave cavity (32); The surface of the concave cavity body is further provided with a functional layer of a non-specific cell adhesion factor, the non-specific cell adhesion factor is polylysine, and the non-specific cell adhesion factor forms a coating on the concave cavity body by coating or chemical plating.

7. A cleaning method characterized by, The bacteria liquid cleaning device for bacterial or cell samples in claim 6 is used to clean the bacteria liquid containing bacterial or cell samples, The cleaning method comprises the following steps: S1. adding the bacteria liquid into the concave cavity body in a certain amount; S2. the liquid adding assembly (10) adds cleaning liquid into the concave cavity body; S3. after the bacteria liquid is cleaned, the liquid sucking assembly (20) sucks waste liquid from the concave cavity body; S4. steps S2 and S3 are repeated to clean the bacteria liquid for multiple times, and the cleaned bacteria liquid is obtained; S5. the cleaning hole plate (30) containing the cleaned bacteria liquid is subjected to steam drying treatment, and is transferred to a detection area for cell test detection.

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