Glass slide and method of using the same

Abstract

The present invention relates to the field of glass slide technology, and specifically discloses a glass slide and a method for using the same. The glass slide includes a substrate, a cover plate and an adhesive layer, wherein the cover plate is provided with a sample loading groove connecting the upper and lower sides of the cover plate; the groove wall of the sample loading groove is recessed inwardly in the horizontal direction to form a storage groove connecting the upper and lower sides of the cover plate; wherein the adhesive layer covers the lower surface of the cover plate, and the groove edge of the storage groove can press a part of the slide onto the substrate via the adhesive layer, and the storage groove can connect the other part of the slide with the outside world. In the present invention, the slide is first pasted on the cover plate through the adhesive layer, and then placed on the substrate together with the cover plate, which can improve the production efficiency of the slide with the slide fixed thereon, and reduce the time consumed when manually pasting each slide into the hole of the slide one by one; at the same time, the slide is directly pasted on the cover plate through the adhesive layer, which can avoid the glue running phenomenon when manually dripping glue, thereby improving the accuracy of sample detection.

Description

Technical Field

[0001] The present invention relates to the technical field of glass slides, and in particular to a glass slide and a use method thereof. Background Art

[0002] When testing samples based on cell substrates, it is necessary to place slides on the slide. This process is relatively cumbersome and requires manual pasting of each slide into the holes of the slide one by one, which affects the production efficiency of the slides with the slides fixed thereon. In addition, during the placement of the slides, the amount of glue added to the slide is not easy to control. When the slides are fixed on the slide by glue, glue is prone to run, which in turn affects the detection of the samples and causes detection errors. When clinicians combine the test results, it is easy to cause clinical misdiagnosis. Summary of the Invention

[0003] The object of the present invention is to provide a glass slide and a method for using the same, so as to solve the problem in the prior art that the production efficiency of the glass slide with the climbing sheet fixed thereon is low and the glue is easily run off when the climbing sheet is fixed on the glass slide by glue.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] In one aspect, the present invention provides a glass slide comprising a substrate and:

[0006] The cover plate is provided with a sample adding slot communicating with the upper and lower sides of the cover plate, the sample adding slot being used for adding samples; the slot wall of the sample adding slot is recessed inwardly along the horizontal direction to form a storage slot communicating with the upper and lower sides of the cover plate;

[0007] An adhesive layer is provided on the lower surface of the cover plate and is used for adhering the climbing sheet to the substrate. The groove edge of the storage groove can press a part of the climbing sheet against the substrate through the adhesive layer, and the storage groove can connect the other part of the climbing sheet to the outside world.

[0008] Furthermore, the above-mentioned storage grooves are provided in plurality, and the plurality of storage grooves are evenly arranged on the periphery of the above-mentioned sample loading groove.

[0009] Furthermore, the storage groove is a circular groove.

[0010] Furthermore, the diameter of the circular groove is smaller than the diameter of the climbing plate.

[0011] Furthermore, the cover plate is a polygonal structure, and a first chamfer is provided at one of the multiple vertex corners of the cover plate.

[0012] Furthermore, the substrate is a rectangular structure, and a holding portion is provided at one end of the substrate along the length direction.

[0013] Furthermore, the glass slide further includes a protective film, which is attached to the upper surface of the cover plate and is used to seal the sample loading slot and the object storage slot.

[0014] Furthermore, the protective film of the above-mentioned slide glass is provided with a film tearing portion.

[0015] In another aspect, the present invention provides a method for using a glass slide, wherein the method is performed using the glass slide as described above, and the method comprises the following steps:

[0016] S1. Fixing the slide: transferring the slide containing cells after recombinant vector transfection or drug treatment to the storage tank of the glass slide through a limiting mold;

[0017] S2. Incubate the sample: add a sufficient amount of the diluted sample to the sample loading tank so that the sample in the sample loading tank can flow into the storage tank, and then place the entire slide in a constant temperature and moisture incubator to incubate the sample;

[0018] S3. Rinse the sample: aspirate the sample in the sample tank, then add a sufficient amount of rinsing liquid to the sample tank for rinsing, and the number of rinsing times is set according to demand;

[0019] S4. Incubate with secondary antibody: remove the rinse solution in the sample loading tank, then add a sufficient amount of secondary antibody to the sample loading tank, and then place the entire slide in a constant temperature and moisture incubator for incubation with secondary antibody;

[0020] S5. Rinsing the secondary antibody: aspirate the secondary antibody in the sample loading tank, then add a sufficient amount of rinsing solution to the sample loading tank for rinsing, and the number of rinsing times is set according to needs, and finally the rinsing solution fills the storage tank in the slide;

[0021] S6. Observation results: The above-mentioned slide is moved horizontally onto a detection instrument, and the detection instrument is used for observation to obtain the observation results.

[0022] In another aspect, the present invention provides a method for preparing a glass slide, comprising the following steps:

[0023] S11, placing the above-mentioned climbing sheet on the above-mentioned limiting mold, with the side of the above-mentioned climbing sheet containing cells facing outward;

[0024] S12, placing the cover plate covered with the adhesive layer on the limiting mold so that the curved edge of the climbing sheet is adhered to the adhesive layer;

[0025] S13, pasting the substrate onto the adhesive layer so that the portion of the climbing sheet not pasted by the adhesive layer can be located in the storage groove.

[0026] The beneficial effects of the present invention are:

[0027] The glass slide includes a substrate, a cover plate and an adhesive layer, and the substrate and the cover plate can be adhered to each other through the adhesive layer to form a glass slide. Among them, the adhesive layer can also be used to stick the climbing sheet, and the groove edge of the cover plate's storage groove can press a part of the climbing sheet against the substrate through the adhesive layer, thereby completing the fixation of the climbing sheet and preventing the climbing sheet from shifting and affecting the detection of the sample. The climbing sheet is first pasted on the cover plate through the adhesive layer, and then placed on the substrate together with the cover plate, which can improve the production efficiency of the glass slide with the climbing sheet fixed, and reduce the time consumed when manually pasting each climbing sheet one by one into the hole of the glass slide. At the same time, the climbing sheet is directly pasted on the cover plate through the adhesive layer, which can avoid the phenomenon of glue running when manually dripping glue, thereby improving the accuracy of sample detection.

[0028] The method for using the glass slide of the present invention is completed by using the above-mentioned glass slide, and the method for using the glass slide can accurately detect the cultivation status of the sample. At the same time, the method for preparing the glass slide is simple and convenient in operation, and the glass slide preparation efficiency is high, thereby improving production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 A schematic structural diagram of a glass slide provided in an embodiment of the present invention;

[0030] Figure 2 An exploded view of a glass slide provided in an embodiment of the present invention;

[0031] Figure 3 A structural schematic diagram of a glass slide provided in an embodiment of the present invention having a protective film attached thereto.

[0032] In the picture:

[0033] 1. Substrate; 11. Holding portion; 12. Second chamfer; 2. Cover plate; 21. Sample loading slot; 22. Storage slot; 23. First chamfer; 3. Adhesive layer; 4. Sheet climbing; 5. Protective film; 51. Film tearing portion. DETAILED DESCRIPTION

[0034] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0035] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and are not intended to indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions, and the first feature being "above", "above" and "above" the second feature includes the first feature being directly above and obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature includes the first feature being directly below and obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0036] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0037] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.

[0038] This embodiment provides a glass slide, which is used for sample detection.

[0039] like Figures 1-2As shown, the slide comprises a substrate 1, a cover plate 2 and an adhesive layer 3. The adhesive layer 3 covers the lower surface of the cover plate 2 and is used to adhere the climbing sheet 4 to the substrate 1, so that the substrate 1 and the cover plate 2 can be adhered to each other through the adhesive layer 3 to form a slide. Among them, the cover plate 2 is provided with a sample loading groove 21 connecting the upper and lower sides of the cover plate 2, and the sample loading groove 21 is used to add samples. The groove wall of the sample loading groove 21 is recessed inwardly in the horizontal direction to form a storage groove 22 connecting the upper and lower sides of the cover plate 2, and the sample added to the sample loading groove 21 can flow into the storage groove 22 from the sample loading groove 21, so that the climbing sheet 4 in the storage groove 22 can be covered with samples, so that the cells on the climbing sheet 4 can be further tested after being placed in the sample. At the same time, when adding samples separately to each climbing sheet 4, the impact force during the adding process prevents the cells on the climbing sheet 4 from falling off, resulting in failure of sample detection. Optionally, the surface of the sample storage tank 22 is treated with a hydrophilic coating to promote uniform flow of the sample from the sample loading tank 21 to each storage tank 22. Furthermore, optionally, multiple storage tanks 22 are provided, and the multiple storage tanks 22 are evenly arranged around the periphery of the sample loading tank 21 to facilitate uniform flow of the sample from the sample loading tank 21 to the storage tank 22, thereby meeting the high precision and consistency requirements of sample detection. The above process is simple to operate, and when adding samples, they only need to be added to the sample loading tank 21, thereby preventing the occurrence of missed or over-addition of samples, thereby improving the efficiency of sample detection.

[0040] Specifically, the groove edge of the storage groove 22 can press a part of the climbing sheet 4 against the substrate 1 through the adhesive layer 3, thereby completing the fixation of the climbing sheet 4, preventing the climbing sheet 4 from shifting and affecting the detection of the sample, and ensuring that the position of each climbing sheet 4 is fixed, as well as the consistency of the horizontal height between the climbing sheets 4, providing a high-precision physical position for detection. The climbing sheet 4 is first pasted on the cover plate 2 through the adhesive layer 3, and then placed on the substrate 1 together with the cover plate 2, which can improve the production efficiency of the slide with the climbing sheet 4 fixed, and reduce the time consumed when manually pasting each climbing sheet 4 one by one into the hole of the slide. At the same time, the climbing sheet 4 is directly pasted on the cover plate 2 through the adhesive layer 3, which can avoid the glue running phenomenon when manually dripping glue, thereby improving the accuracy of sample detection. Among them, the storage groove 22 can connect the other part of the climbing sheet 4 with the outside world, so that the cells on the climbing sheet 4 can contact the sample in the storage groove 22 to complete the detection of the sample. The substrate 1 is a transparent glass sheet, and using the transparent glass sheet as a glass slide for the substrate 1 can facilitate the detection instrument to detect the sample in the glass slide.

[0041] Furthermore, the number of sample slots 22 and the number of slides 4 can be increased or decreased according to the requirements of the test project to adapt to various test situations. At the same time, multiple sample loading slots 21 can be opened on a glass slide, and the periphery of each sample loading slot 21 is provided with a sample slot 22 similar to the above-mentioned arrangement. Then, by adding samples to each sample loading slot 21, the sample processing speed can be greatly improved and the loss of samples during processing can be reduced.

[0042] Furthermore, the placement groove 22 is a circular groove. When the arc-shaped groove edge of the circular groove presses a part of the climbing sheet 4 against the substrate 1 through the adhesive layer 3, the area of ​​the upper surface of the part of the climbing sheet 4 that is not pressed can be large enough, so that there can be a sufficient number of cells on the climbing sheet 4, while avoiding the cells on the climbing sheet 4 being covered by the adhesive layer 3 and affecting the detection of the sample. Of course, the placement groove 22 can also be other shapes that can ensure that the climbing sheet 4 will not be affected after it is fixed, and this embodiment does not specifically limit it. Optionally, the diameter of the circular groove is smaller than the diameter of the climbing sheet 4, so that the arc-shaped groove edge of the circular groove can press the arc-shaped edge of the climbing sheet 4 that is long enough, and when it does not affect the cells on the climbing sheet 4, it can also ensure the stability of the climbing sheet 4 fixed in the slide, and avoid the sliding sheet 4 from falling off during the movement of the slide.

[0043] Optionally, the cover plate 2 is a polygonal structure, and a first chamfer 23 is provided at one of the multiple top corners of the cover plate 2. The first chamfer 23 can be used to locate the position of the storage slot 22, so that the staff can distinguish the position of each storage slot 22, and prevent the misplacement of the climbing slice 4 and the sample. Further optionally, the substrate 1 is a rectangular structure, and a holding portion 11 is provided at one end of the substrate 1 along the length direction. The holding portion 11 can facilitate the staff to take the slide. At the same time, a second chamfer 12 is provided at the top corner of the other end of the substrate 1 along the length direction. The second chamfer 12 on the substrate 1 and the first chamfer 23 on the cover plate 2 can overlap in the vertical direction, and then the slide can be positioned by the first chamfer 23 and the second chamfer 12 and placed on the reagent rack. Specifically, the holding portion 11 protrudes from the cover plate 2 at one end of the substrate 1 along the length direction, and then the staff can avoid contamination of the slide by taking the slide through the holding portion 11. In specific implementation, the size of the glass slide in this embodiment can be set to the same size as a conventional, universal glass slide, so there is no need to take out the slide 4 separately for observation, and the entire glass slide can be directly placed on the stage of the detection instrument.

[0044] like Figure 3As shown, the glass slide also includes a protective film 5, which is attached to the upper surface of the cover plate 2 and is used to block the sample loading slot 21 and the storage slot 22 to prevent contamination of the glass slide during movement or transportation. Optionally, a tear-off portion 51 is provided at one end of the protective film 5 along the length direction. Specifically, the tear-off portion 51 is provided at one end of the protective film 5 along the length direction, protruding from the cover plate 2, thereby making it convenient for personnel to tear off the protective film 5 through the tear-off portion 51.

[0045] This embodiment also provides a method for using a glass slide. The method for using the glass slide is completed using the glass slide as described above and is used for sample detection. The method for using the glass slide includes the following steps:

[0046] S1, fixing the slide 4: transferring the slide 4 containing cells after recombinant vector transfection or drug treatment to the storage tank 22 of the slide through a limiting mold;

[0047] S2. Incubate the sample: Add a sufficient amount of diluted sample to the sample adding tank 21 so that the sample in the sample adding tank 21 can flow into the storage tank 22, and then place the entire slide in a constant temperature and moisture incubator to incubate the sample;

[0048] S3, rinsing the sample: sucking the sample in the sample adding tank 21, and then adding a sufficient amount of rinsing liquid to the sample adding tank 21 for rinsing, and the number of rinsing times is set according to demand;

[0049] S4, incubating with secondary antibody: aspirate the rinse solution in the sample loading tank 21, then add a sufficient amount of secondary antibody to the sample loading tank 21, and then place the entire slide in a constant temperature and moisture incubator for incubation with secondary antibody;

[0050] S5. Rinsing the secondary antibody: The secondary antibody in the sample loading tank 21 is sucked away, and then a sufficient amount of rinsing solution is added to the sample loading tank 21 for rinsing. The number of rinsing times is set according to the needs, and finally the rinsing solution fills the storage tank 22 in the slide glass;

[0051] S6. Observation results: The slide is moved horizontally onto the detection instrument, and the detection instrument is used for observation to obtain the observation results.

[0052] The method for using the glass slide provided in this embodiment can accurately detect the cultivation status of the sample. The staff adds the sample in the sample adding slot 21 so that the sample can flow from the sample adding slot 21 into the storage slot 22, so that the climbing sheet 4 in the storage slot 22 can be covered with the sample, which is convenient for further testing. At the same time, the method for using the glass slide is simple and convenient in operation, and the detection efficiency of the sample is high. When adding the sample, it only needs to be added in the sample adding slot 21, so that there will be no situation of missing or adding too many samples, and the results after the test are highly referenceable. In steps S3 to S5, when the liquid in the sample adding slot 21 is sucked away by using a dropper, due to the fluidity of the liquid, the liquid in the storage slot 22 can flow from the storage slot 22 into the sample adding slot 21, so that the staff can also suck away the liquid in the storage slot 22.

[0053] The present invention also provides a method for preparing a glass slide, and the preparation steps of the glass slide include the following:

[0054] S11, placing the climbing sheet 4 on the limiting mold, with the side of the climbing sheet 4 containing cells facing outward;

[0055] S12, placing the cover plate 2 covered with the adhesive layer 3 on the limiting mold so that the curved edge of the climbing sheet 4 is adhered to the adhesive layer 3;

[0056] S13 , pasting the substrate 1 on the adhesive layer 3 so that the portion of the climbing sheet 4 not pasted by the adhesive layer 3 can be located in the storage groove 22 .

[0057] During steps S11 to S13, multiple climbing sheets 4 are first pasted on the cover plate 2 through the adhesive layer 3, and then placed on the substrate 1 together with the cover plate 2. This can improve the production efficiency of the glass slides fixed with the climbing sheets 4, and at the same time reduce the time consumed in manually pasting each climbing sheet 4 one by one into the hole of the glass slide, thereby facilitating the production of the glass slides fixed with the climbing sheets 4.

[0058] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description. It is not necessary and impossible to enumerate all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.

Claims

1. A slide glass, characterized in that: It comprises a substrate (1) and: A cover plate (2), the cover plate (2) is provided with a sample adding groove (21) communicating with the upper and lower sides of the cover plate (2), the sample adding groove (21) being used for adding samples; the groove wall of the sample adding groove (21) is recessed inwardly along the horizontal direction to form a storage groove (22) communicating with the upper and lower sides of the cover plate (2); An adhesive layer (3), the adhesive layer (3) covering the lower surface of the cover plate (2) and used for adhering the climbing sheet (4) to the base plate (1); the groove edge of the storage groove (22) can press a portion of the climbing sheet (4) against the base plate (1) via the adhesive layer (3), and the storage groove (22) can connect another portion of the climbing sheet (4) to the outside world; The climbing sheet (4) is directly adhered to the cover plate (2) via the adhesive layer (3); The storage groove (22) is a circular groove, and the arc-shaped groove edge of the circular groove presses a part of the climbing sheet (4) onto the base plate (1) via the adhesive layer (3); the diameter of the circular groove is smaller than the diameter of the climbing sheet (4).

2. The slide glass according to claim 1, wherein A plurality of the storage slots (22) are provided, and the plurality of storage slots (22) are evenly arranged on the periphery of the sample adding slot (21).

3. The slide glass according to claim 1, wherein The adhesive layer (3) is a polyparaxylene nano coating.

4. The slide glass according to claim 1, wherein The cover plate (2) is a polygonal structure, and a first chamfer (23) is provided at one of the multiple top corners of the cover plate (2).

5. The slide glass according to claim 1, wherein The substrate (1) is a rectangular structure, and a holding portion (11) is provided at one end of the substrate (1) along the length direction.

6. The slide glass according to claim 1, wherein The slide also includes a protective film (5), which is attached to the upper surface of the cover plate (2) and is used to seal the sample loading slot (21) and the object storage slot (22).

7. A method for using a slide, characterized in that: The method for using the glass slide is completed using the glass slide according to any one of claims 1 to 6, and the method for using the glass slide comprises the following steps: S1, fixing the crawling sheet (4): transferring the crawling sheet (4) containing cells after recombinant vector transfection or drug treatment to the storage groove (22) of the slide through a limiting mold; S2. Incubate the sample: add a sufficient amount of the diluted sample into the sample adding tank (21) so that the sample in the sample adding tank (21) can flow into the storage tank (22), and then place the entire slide in a constant temperature and moisture incubator to incubate the sample; S3, rinsing the sample: sucking away the sample in the sample adding tank (21), and then adding a sufficient amount of rinsing liquid to the sample adding tank (21) for rinsing, and the number of rinsing times is set according to demand; S4, incubating the secondary antibody: aspirating the rinse solution in the sample loading tank (21), then dripping a sufficient amount of the secondary antibody into the sample loading tank (21), and then placing the entire glass slide in a constant temperature and moisturizing incubator to incubate the secondary antibody; S5, rinsing the secondary antibody: sucking away the secondary antibody in the sample loading tank (21), then dripping a sufficient amount of rinsing liquid into the sample loading tank (21) for rinsing, and the number of rinsing times is set according to demand, and finally the rinsing liquid fills the storage tank (22) in the slide glass; S6. Observation results: The glass slide is translated onto a detection instrument, and the detection instrument is used for observation to obtain observation results.

8. The method for using a glass slide according to claim 7, wherein: In step S1, it includes: S11, placing the climbing sheet (4) on the limiting mold, with the side of the climbing sheet (4) containing cells facing outwards; S12, placing the cover plate (2) covered with the adhesive layer (3) on the limiting mold, so that the arc-shaped edge of the climbing sheet (4) is adhered to the adhesive layer (3); S13, pasting the substrate (1) onto the pasting layer (3) so that the portion of the climbing sheet (4) not pasted by the pasting layer (3) can be located in the storage groove (22).

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